International Journal of Electrochemical Science
·
2023
·
IF 1.5
Qiu-feng Lan,Wen-chang Wang,Xiao-hui Chen,Zhi-dong Chen
The poly(3-amino-4-hydroxybenzenesulfonic acid)/Ru(bpy) 3 2+ modified glassy carbon electrode (PAHBS/Ru(bpy) 3 2+ /GCE) is a novel solid-state electrochemiluminescence (ECL) sensor. It was prepared by electropolymerization of 3-amino-4-hydroxybenzenesulfonicacid (AHBS) followed by soaking into Ru(bpy) 3 2+ solution. Ru(bpy) 3 2+ was entrapped on the electrode via the electrostatic interactions between sulfonic acid groups and Ru(bpy) 3 2+ cation. The morphology and composition of the modified electrodes were analysed using scanning electron microscope and energy-dispersed spectrum. Cyclic voltammetry and electrochemical impedance spectroscopy were used to compare the electrochemical behavior of the electrodes before and after modification. Under the optimal conditions, the ECL intensity of the quenching value (ΔECL) versus the logarithm of the concentration of malachite green (MG) was linear over a concentration range from 5.0 × 10 -8 to 1.0 × 10 -5 mol L -1 ( r = 0.9931) and the limit of detection (LOD) was 2.5 × 10 -8 mol L -1 . This method has been successfully applied to the determination of MG in pond water.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Haifeng Yao,Yahui Zhang,Yongping Dong,Mingfu Ye
Graphdiyne (GDY) is considered as another promising carbon material in biosensing field. However, the application of GDY in ECL sensing is in its infancy stage, and different roles of GDY are urgent to be discovered. In this work, strong anodic Ru(bpy) 3 2+ electrochemiluminescence (ECL) was obtained at a cucurbit[8]uril (Q[8]) modified electrode. Although GDY exhibited electrocatalytic effect on the oxidation of Ru(bpy) 3 2+ , an apparent inhibiting effect of GDY on the ECL signal was observed due to the energy transfer. An “on-off-on” mode ECL sensor was fabricated to detect pancuronium (PMD) based on the competitive host–guest interaction between ferrocene (Fc), PMD and Q[8]. Fc modified single-stranded DNA was immobilized onto the surface of GDY, which could be attached onto the electrode through the interaction between Fc and Q[8]. The quenching effect of GDY could apparently decrease ECL signal. PMD exhibited stronger combining ability with Q[8] than Fc, and could replace Fc from the cavity of Q[8], leading to the recovery of ECL signal. The variation of ECL intensity changed linearly with the PMD concentration in the range of 10 nM to 50 μM with a detection limit of 5 nM (3σ). The proposed sensor has high sensitivity, outstanding specificity and accuracy, indicating that GDY has promising potential application in ECL sensing field based on its inhibiting effect.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Ziying Zhong,Xiaoshuo Zhang,Rui Dai,Chunfang Wang,Faqiong Zhao,Baizhao Zeng
In this work, layered Co-Ni bimetallic hydroxide was synthesized and combined with polyethyleneimine hydrogel (PEI@LDH) as substrate material, then the metal-organic framework MIL-125 loaded Ru(bpy) 3 2+ (MIL@Ru) was added as single luminophore, followed by electrochemical deposition of tetracycline (TC) imprinted copolymer of hydroquinone and o-phenylenediamine to construct an electrochemiluminescent (ECL) sensor for TC detection. The stable and sensitive luminophore MIL@Ru associating with co-reactant K 2 S 2 O 8 in the solution could cause a cathode ECL signal sensing TC, it could also associate with the immobilized co-reactant PEI hydrogel to produce a stable anode ECL signal acting as a reference. The LDH had catalysis and could efficiently amplify both anode and cathode ECL signals. Therefore, the constructed single luminophore based ratiometric ECL sensor displayed high selectivity, sensitivity and reproducibility. Under the selected testing conditions, the linear response range was 0.01 μmol L −1 to 1 mmol L −1 and the detection limit reached 8 nmol L −1 . Its good practicability was validated by determining TC in different actual samples. Hence, the ratiometric MIP-ECL sensor was quite promising.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yuye Li,Dong Liu,Shuyun Meng,Jiayi Zhang,Libo Li,Tianyan You
Ferrocene (Fc) is a common quencher of Ru(bpy)32+ luminescence. However, interactions between Fc and Ru(bpy)32+ can be extremely complicated. In this work, we reported the first use of Fc to regulate the electrochemiluminescence (ECL) of Ru(bpy)32+ by tuning the length of the DNA sequence between Fc and the luminophore of nitrogen-doped graphene quantum dots-Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru-NGQDs). The ECL of SiO2@Ru-NGQDs was depressed when the distance between Ru(bpy)32+ and Fc was less than 8 nm; a stronger ECL was observed when the distance was more than 12 nm. The switching of the ECL of Ru(bpy)32+ by Fc was attributed to the electron transfer mechanism, in which Fc participated in the redox of Ru(bpy)32+ for “signal-off” ECL; this favored electron transfer at the electrode fabricated with an Fc-labeled aptamer (Fc-apt) and SiO2@Ru-NGQDs for “signal-on” ECL depending on the length of the DNA sequence. Here, a dual-signal readout aptasensor for aflatoxin B1 (AFB1) detection was developed via the enhanced ECL of SiO2@Ru-NGQDs by Fc-apt. The redox currents of Fc and the ECL of Ru(bpy)32+ were simultaneously collected as yardsticks, and both decreased with higher concentrations of AFB1. The aptasensor allowed linear ranges of 3 × 10–5 to 1 × 102 ng mL–1 for ECL mode and 1 × 10–3 to 3 × 103 ng mL–1 for electrochemical mode. Our work provides insight into the interactions between Fc and Ru(bpy)32+. The dual-signal readout strategy is a potential platform for the versatile design of aptasensors.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Muhammad Saqib,Shahida Bashir,Sajjad Ali,Rui Hao
Developing highly specific, rapid, and cost-effective electrochemiluminescence (ECL) methods capable of detecting multiple analytes remains a compelling research goal but it is still a substantial challenge due to narrow choice of ECL systems. In this study, efficient ECL detections were achieved by using anodic Ru(bpy) 3 2+ -acridine orange (AO) ECL system. In present system, dimethylamino substituent on acridine ring acts as active sites for strong ECL generation, while heterocyclic nitrogen atom (–N–) contributes to specific complex formation with metal ion. Accordingly, this anodic ECL method was exploited for highly sensitive detection of both mercury (II) (Hg 2+ ) and dopamine (DA), with detection limits as low as 0.36 nM (linear range 0.01–50 μM) and 1.0 nM (linear range 0.01–10 μM), respectively. The mechanism of Hg 2+ detection was supported by density functional theory (DFT) calculations. Notably, the ECL method exhibit superior selectivity over numerous competing interfering agents. Moreover, the method achieved the detection of both targets in practical media with excellent recoveries (96.0–103.8%). Importantly, the method demonstrates potential advantages in terms of selectivity, sensitivity, rapidity, simplicity, and low cost. This work realizes a successful attempt to further expand the analytical applications of ECL systems and may initiate new thoughts in utilizing acridines in diverse ECL-based sensing and imaging applications.
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Baohan Wen,Haoyi Ren,Wenjing Lai,Min Wang,Mingzhe Jiang,Yingying Cheng,Yige Li,Chenglin Hong,Yu Qi
Early cancer diagnosis is crucial due to a significant increase in cancer incidence over the last three decades. A highly sensitive electrochemiluminescence technique was developed for carcinoembryonic antigen (CEA) detection. This method uses Ru(bpy)32+ as the luminescent substance, and the silica and polyacrylamide gel composite as the carrier. Ru(bpy)32+ is loaded into it to prevent its leakage. At the same time, biological molecules such as antibodies or antigens are immobilized, thereby improving the stability and reaction activity of the system. Additionally, Au-Fc-MOF was synthesized to act as a quencher and secondary antibody label, absorbing the emitted energy from the ECL emitter to improve detection accuracy across various CEA concentrations. Potassium persulfate was employed as a co-reactant to generate SO4•- radicals, intensifying the luminescence output of the system. The findings revealed that the developed immunosensor exhibited strong linearity within the concentration range of 10-5 ng/mL to 10 ng/mL, with a remarkably low detection limit of 5.03 fg/mL. This electrochemical luminescence immunosensor, characterized by its simple preparation, cost-effectiveness, and superior performance, demonstrates significant potential in advancing early CEA detection methodologies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Liangrui Lv,QingQing Chen,Chunyang Jing,Xiaoying Wang
To sensitively monitor trace-level of toxic patulin (PAT), an ultrasensitive PAT ratiometric aptasensor based on the dual-potential electrochemiluminescence (ECL) of Ru(bpy) 3 2+ was first proposed. Noteworthily, Ru(bpy) 3 2+ -doped trimetallic nanocube ( [email protected] ) innovatively integrated the luminophore and cathode coreaction accelerator (CCA), which could generate strong cathodic ECL in the existence of low concentration of K 2 S 2 O 8 . Simultaneously, anthocyanin-derived carbon quantum dots (anth-CQDs) prepared from purple potato skins was first served as a green anodic coreactant. And SiO 2 -coated anth-CQDs ( [email protected] 2 ) exhibited excellent performance for enhancing anodic ECL of [email protected] Based on this, a novel ternary ECL system was established. In the presence of PAT, the ECL intensity ratio of anode to cathode ( I ECL-A / I ECL-C ) was significantly increased, and a low detection limit of 0.05 pg mL −1 was obtained. Moreover, when proposed method and high performance liquid chromatography (HPLC) were simultaneously applied to series of fruit products, the obtained results were completely consistent, reflecting its practicability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiu-hua Wei,Xing Qiao,Jie Fan,Yuan-qiang Hao,Yin-tang Zhang,Yan-li Zhou,Mao-tian Xu
Herein, a label-free electrochemiluminescence (ECL) biosensor for sensitive determination of carcinoembryonic antigen (CEA) was fabricated based on CdS quantum dots loaded onto metal organic framework (CdS QDs@MOF) and triethanolamine modified on gold nanoparticles (TEOA@AuNPs) as bi-coreactants of Ru(bpy) 3 2+ ECL system. The TEOA@AuNPs served as not only coreactant of Ru(bpy) 3 2+ but also nanocarrier for CEA aptamer which was bonded to the TEOA@AuNPs through Au–S bond. After incubated in CEA solution, the ECL signals decreased due to the poor conductivity of CEA and there was a linear correlation between the ECL intensity and log(C CEA ). Based on this result, a label-free ECL biosensor has been developed successfully and used to detect CEA with a detection limit of 8.5 × 10 −5 ng/mL. The proposed ECL aptasensor had good analytical performance in the detection of CEA in human serum samples.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2016
·
IF 5.886
Yong-Ping Dong,Ying Zhou,Jiao Wang,Jun-Jie Zhu
In the present work, strong anodic electrogenerated chemiluminescence (ECL) of Ru(bpy)32+ was observed at a graphene oxide modified glassy carbon electrode (GO/GCE) in the absence of coreactants. The electrocatalytical effect of GO on the oxidation of Ru(bpy)32+ suggested that GO itself can act as the coreactant of Ru(bpy)32+ ECL, which can be used to fabricate the ECL biosensor. Thiol group terminated adenosine triphosphate (ATP) aptamer was immobilized on the GO film via DNA hybridization. When gold nanoparticles/graphene oxide (AuNPs/GO) nanocomposites were modified on the aptamer through the S–Au bond to form a sandwich-like structure, the ECL resonance energy transfer (ECL-RET) could occur between Ru(bpy)32+ and AuNPs/GO nanocomposites, resulting in an apparent decrease of ECL signal. After the ECL sensor was incubated in ATP solution, the AuNPs/GO nanocomposites were released from the electrode due to the specific interaction between aptamer and ATP, leading to the increased ECL signal. On the basis of these results, an ECL aptasensor was fabricated and could be used in the sensitive and selective detection of ATP in the range of 0.02–200 pM with a detection limit of 6.7 fM (S/N = 3). The proposed ECL aptasensor can be applied in the detection of ATP in real samples with satisfactory results.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Xiaoshuo Zhang,Rui Dai,Faqiong Zhao,Baizhao Zeng
Background Epithelial cell adhesion molecules (EpCAM) protein is one of the most frequently and intensely studied tumor-associated antigens because of its overexpression in most cancer cells. Thus, it is regarded as a tumor biomarker associated with a wide variety of carcinomas and reflects the existence and growth of tumors in the human body. It is highly required to detect low concentrations of EpCAM in early stage for ensuring human health. Results In this study, a novel sandwich electrochemiluminescence (ECL) aptasensor was developed for EpCAM detection. Wherein oxalate-based Zn-MOF supported tris(2,2′-bipyridyl)ruthenium (II) (Ru(bpy) 3 2+ @Zn-MOF) was used as luminophore and gold nanoparticle modified o-phenylenediamine (PoPD@Au NPs) as quencher. The 3D Ru(bpy) 3 2+ @Zn-MOF generated self-enhanced ECL without additional co-reactants. Furthermore, it remained stable and had short electron transfer distance, ensuring high and stable ECL signal. The ECL signal could be effectively quenched by PoPD@Au NPs through resonance energy transfer (RET) and electron transfer (ET), due to its absorption spectrum overlapping with the ECL emission spectrum of Ru(bpy) 3 2+ @Zn-MOF. In the presence of EpCAM, the aptamer acted as a linker between the luminophore and quencher, forming a sandwich structure that had reduced ECL intensity. Under optimal conditions, EpCAM could be sensitively detected over the range of 1 pg mL -1 -100 ng mL -1 , with a detection limit of 0.34 pg mL -1 . Significance This work developed a ECL aptasensor with self-enhanced Ru(bpy) 3 2+ @Zn-MOF luminophore for EpCAM detection. The ECL system was simple, but ultrasensitive for EpCAM monitoring, thanks to the ECL-RET and ET between Ru(bpy) 3 2+ @Zn-MOF and PoPD@Au NPs. It also could be applied to detect other biomolecules by replacing the aptamer, offering significant potential in disease diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2325-2331
·
IF 4.58
Huang Xinan,Jiang Hong,Li Yongxin,Sang Lijia,Zhou Huipeng,Shahzad Sohail Anjum,Ibupoto Zafar Hussain,Yu Cong
A sensitive and selective luminescent nanoprobe (referred to as DEPN) is designed for the determination of Cu(II) ions. DEPN shows two emission peaks, one at 602 nm and caused by [Ru(bpy) 3 ] 2+ , the other (peaking at 500 nm) caused by silver nanoclusters (AgNCs). The luminescence of the ruthenium complex is inert and acts as the internal reference signal, while that of the AgNCs is quenched by Cu(II) ions. Under 350 nm photoexcitation, a linear relationship is found between the ratio of emission intensity (I 500 /I 602 ) and the concentration of Cu(II) ions in the 0.1–16 μM range. The detection limit of Cu(II) ions is estimated to be 50 nM. The DEPN was applied to the determination of Cu(II) ions in (spiked) water samples, and to intracellular imaging of Cu(II) in HeLa cells using confocal fluorescence microscopy.
Schematic presentation of a dual-emission photoluminescence nanoprobe for selective Cu 2+ sensing. The emission of the Ag nanoclusters (at 500 nm) is quenched by Cu 2+ , while that of the Ru(bpy) 3
2+ -doped silica nanoparticles (at 602 nm) remains unchanged and serves as a reference.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 4
·1-10
·
IF 5.3
Shan Xiangyu,Lu Juan,Li Chao,Wu Qian,Li Huiling,Yang Shuning,Guo Yanjia,Song Yujia,Li Ruidan,Tian Li
An efficient and innovative electrochemiluminescence (ECL) sensor was developed for trace detection of cyfluthrin. The sensor utilized materials such as lotus root shaped carbon fiber (Co CNFs), cadmium selenide quantum dots (CdSe QDs), and Fe 3 O 4 to amplify Ru(bpy) 3 2+ signals. Co CNFs, with its large specific surface area and porosity, served the purpose of not only enhancing the stability of the sensor by fixing CdSe QDs and Ru(bpy) 3 2+ on the Co CNFs/GCE, but also facilitating electron transfer. CdSe QDs was involved in the luminescence reaction and collaborated with Ru(bpy) 3 2+ to enhance the sensor's sensitivity, while Fe 3 O 4 promoted electron transfer in the system due to its large surface area. The solid-state ECL sensor achieved satisfactory signal under the synergistic action of these components. The ECL signal of the sensor was quenched by cyfluthrin, and a favorable linear relationship was observed between the sensor and cyfluthrin in the concentration range 1 × 10 –12 to 1 × 10 –6 M. The detection limit of the sensor was 3.3 × 10 –13 M (S/N = 3). The utilization of lotus root shaped carbon fiber, CdSe QDs, and Fe 3 O 4 in the Ru(bpy) 3 2+ system demonstrated a synergistic effect for cyfluthrin detection, presenting a new approach for the rapid determination analysis of pesticide residues in foods. Graphical
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）