ANALYST
·
2023
·Vol. 148
·期 9
·2100-2109
·
IF 4.2
Yanxue Xu,Guihua Chen,Yunting Qin,Dan Xiao
A glassy carbon electrode (GCE) was modified with graphite powder (G/GCE), and then treated with a solution of sodium peroxide for several minutes to prepare an oxidized G/GCE (OG/GCE). The OG/GCE has prominently improved responses toward dopamine (DA), rutin (RT) and acetamidophenol (APAP), of which the anodic peak current was increased by 2.4, 4.0 and 2.6 fold compared with that obtained using the G/GCE. The redox peaks of DA, RT and APAP on the OG/GCE could be separated sufficiently. The corresponding redox processes were confirmed as diffusion-controlled and parameters such as the charge transfer coefficients (α), saturating adsorption capacity (Γ*) and catalytic rate constant (kcat) were estimated. In individual detection, the linear ranges for DA, RT and APAP were 10 nM–10 μM, 1.00 nM–150 nM and 20 nM–30 μM, respectively, and the LODs for DA, RT and APAP were estimated as 6.23 nM, 0.36 nM and 13.1 nM with 3σ/S, respectively. The contents of RT and APAP in drugs were determined and agreed with the labeled contents. The recoveries of DA in serum and sweat were in the range of 91–107%, indicating that the determination results achieved by the OG/GCE are reliable. The practical use of the method was verified with a graphite-modified screen-printed carbon electrode (G/SPCE), which was also activated by Na2O2 to prepare OG/SPCE. The recovery of DA in sweat achieved using the OG/SPCE was 91.26%.
研究领域:
电化学
·
分析化学
·
材料科学
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DOI
PubMed
高影响因子
Chinese Herbal Medicines
·
2016
·
IF 0
Yu Guo,Li-xian Shen,Yan-feng Lu,Hai-yan Li,Ke Min,Lan-fang Li,Cui-yun Yu,Xing Zheng
Objective To prepare, characterize and evaluate the antioxidant activity of rutin-liposome (RL). Methods Liposomes of rutin were prepared by film dispersion method and the encapsulation efficiency (EE) was determined by RP-HPLC. Human umbilical vein endothelial cells (HUVECs) were injuried by H 2 O 2 and treated with either free aqueous rutin or the RL delivery systems. The viability of HUVECs was determined by MTT and ELISA. Results The drug delivery system showed uniform rutin loaded nanoparticles with average particle size of (147.20 ± 1.42) nm, polydispersity index of (0.191 ± 0.003) nm, Zeta potential of (−20.0 ± 1.0) mV, and the drug EE was closed to 90.0%. The antioxidant effect of the drug delivery system to H 2 O 2 -damaged HUVECs showed that RL could increase injury cells viability compared to free aqueous rutin, which was accompanied with an obvious decrease in malondialdehyde (MDA), lactate dehydrogenase (LDH) while increase the level of nitrogen oxide (NOS). Conclusion The nanostructured RL is improved on the antioxidant effect and may be treating the different diseases caused by free radicals.
研究领域:
药剂学
·
生物医学
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DOI
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2026
·
IF 4
Zigao Chen,Yuteng Fu,Xiuting Hu,Shunjing Luo,Jiangping Ye,Chengmei Liu
BACKGROUND Rutin, a flavonol polyphenol, inhibits α-glucosidase activity and reduces starch digestibility, yet its application is limited by poor aqueous solubility, poor thermal stability, and low bioaccessibility. Rutin–protein nanoparticles have been shown to improve rutin stability. This study aimed to investigate how rutin–protein nanoparticles modulate the digestibility of extruded recombinant rice through multi-scale structural characterization, in vitro digestibility analysis, and in vitro fermentation evaluation. RESULTS Multi-scale structural analysis revealed that rutin was successfully incorporated into the recombinant rice matrix, interacting with starch chains through hydrogen bonding. This interaction promoted the transformation of starch crystallinity from A-type to A- + V-type. Additionally, the short-range order and the content of single and double helices increased. In vitro digestion experiments demonstrated that the bioaccessibility of rutin in the recombinant rice reached 92.49% after co-extrusion with rutin nanoparticles. The resistant starch content was significantly increased, while C ∞ (final digestion extent) and eGI (estimated glycemic index) value were significantly reduced. Moreover, in vitro fermentation results indicated that the recombinant rice with rutin nanoparticles contributed to reducing gas production and increasing the yields of propionate and butyrate. CONCLUSION Rutin nanoparticles modulate starch digestibility through a dual mechanism involving the modification of starch structural domains and rutin bioaccessibility. This study provides a novel strategy and technical support for the development of low-glycemic-index functional staple foods. © 2026 Society of Chemical Industry.
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PubMed
高影响因子
Probiotics and Antimicrobial Proteins
·
2026
·
IF 5.3
Tang Xin,Zhang Shuling,Mao Bingyong,Zhang Qiuxiang,Zhao Jianxin,Chen Wei,Cui Shumao
Rutin shows potential for reducing uric acid (UA) but suffers from low bioavailability. Microbial biotransformation can convert rutin into metabolites with enhanced absorption, stability, and physiological activity. This study aims to investigate the biotransformation effects of probiotics containing α-L-rhamnose glycosidase and α-L-rhamnose glycosidase on rutin, as well as their therapeutic efficacy for hyperuricemia (HUA). In vitro, Lactobacillus gasseri CCFM1346 exhibited significantly higher conversion rates for transforming rutin into quercetin and isoquercetin compared to other strains. In HUA mice, both the CCFM1346-fermented rutin preparation (post-Rutin) and a synbiotic combination (Synbiotic) significantly lowered serum UA and creatinine levels ( p < 0.05). Moreover, the Synbiotics group achieved superior serum UA reduction (74.7%) compared to the post-Rutin group (60.1%), despite more moderate xanthine oxidase inhibition. These treatments effectively decreased hepatic xanthine oxidase and enhanced urate oxidase activity, as well as downregulated mRNA levels of UA reabsorption transporters ( URAT1 and GLUT9 ) while upregulating the efflux transporter ABCG2 . Moreover, the combination of L. gasseri CCFM1346 with rutin significantly inhibited the transcription of renal inflammasome components ( NLRP3 , ASC , Caspase1 ) and inflammatory cytokines ( IL-6 , IL-1β , TNF-α ). Futuremore, correlation analysis indicates that these indicators exhibit a significant positive correlation ( p < 0.001). In conclusion, L. gasseri CCFM1346-fermented rutin alleviates HUA through multi-mechanistic actions, with the synbiotic preparation demonstrating superior efficacy by concurrently targeting UA production, excretion, and inflammation. These findings provide a foundation for developing synbiotic functional foods for UA management.
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Zejun Zhang,Lisi Wang,Brij Mohan,Jiaqi Geng,Lang Li,Siyue Zhang,Wei Sun
Leaf-like ZIF-on-ZIF derived CNTs-reinforced Co–N-doped carbon heterostructure (CoCN-CNTs) was prepared. • CoCN-CNTs modified screen-printed carbon electrode was applied to sensitive rutin detection. • Linear detection range (0.1 to 120.0 μmol L −1 ) and the detection limit (0.033 μmol L −1 ) for rutin were realized. Rutin (RU) is an important dietary flavonoid with numerous health benefits, underscoring the need for rapid, reliable methods to quantify its content in food products. In this study, a portable electrochemical sensor for RU detection was developed using a two-dimensional zeolitic imidazolate framework (ZIF)-on-ZIF derived heterostructure. A leaf-like ZIF (ZIF-L) and cobalt-based ZIF (ZIF-67) precursor, denoted as ZIF-L@ZIF-67, was synthesized and pyrolyzed to form a carbon nanotubes (CNTs) reinforced Co–N-doped carbon heterostructure (CoCN-CNTs). The resulting material was immobilized on a screen-printed carbon electrode and applied to RU detection by differential pulse voltammetry. Owing to its high surface area and abundant Co−N active sites, CoCN-CNTs exhibited excellent electrochemical activity and sensitivity. The modified electrode showed a wide linear response range of 0.1–120.0 μmol L −1 and a low detection limit of 0.033 μmol L −1 for RU. The sensor was successfully applied to the analysis of various fruit samples, achieving satisfactory recoveries and demonstrating strong potential for practical nutritional analysis. This work offers an effective strategy for designing high-activity two-dimensional ZIF-on-ZIF-derived heterostructures for portable electrochemical sensing applications. Download: Download high-res image (116KB) Download: Download full-size image
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高影响因子
Nutrients
·
2025
·Vol. 17
·期 9
·1495
·
IF 5
Xiaofang He,Longfei Ma,Jiaqi Zhang,Binbin Zhou,Shun Chen,Minhang Tu,Gentan Cai,Tian Wang,Chao Wang
In animal production, nanoparticulate zinc oxide exhibits synergistic antibacterial efficacy coupled with growth-promoting effects, positioning itself as a novel antibiotic alternative with enhanced biosafety profiles. However, its dose-dependent toxicity poses challenges.Objective:The experimental design sought to quantify the protective effects of dietary rutin against zinc-overload-induced damage.Methods:A zinc-overload murine model was established by giving high-dose ZnO nanoparticles (HZn, 5000 mg/kg/day) for 21 days. Mice were then fed rutin at doses of 300, 600, or 1200 mg/kg. Body weight, relative organ indexes, zinc concentrations, serum enzyme activities, and tissue-level indicators of apoptosis, autophagy, mitochondrial function, and antioxidant capacity were measured.Results:The results showed that rutin could not reverse HZn-induced body weight decline but improved relative organ indexes in liver and kidney. It alleviated HZn-induced cell damage and enhanced antioxidant capacity in jejunum and serum throughNrf2activation, without inhibiting HZn-induced zinc elevation.Conclusions:Rutin, especially at 600 mg/kg, can partially restore hepatic function and organ index and mitigate HZn-induced hepatic and jejunal injuries.
研究领域:
毒理学
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营养学
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分子生物学
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PubMed
高影响因子
Biomedical Physics & Engineering Express
·
2025
·Vol. 11
·期 5
·055007
·
IF 1.6
Xuehai Dong,Feng Wan,Jingjing Zhang,Can Zhuo,Naxin Zhou
This study aimed to develop a multifunctional hydrogel for skin wound healing by encapsulating rutin-modified molybdenum disulfide (MoS2) within methacrylated hyaluronic acid (HAMA). Rutin, a natural flavonoid, was employed to stabilize MoS2 dispersion and enable sustained release, thereby promoting fibroblast proliferation. Scanning electron microscopy revealed improved dispersion of rutin-modified MoS2 within the HAMA hydrogel matrix. Under near-infrared (NIR) irradiation, MoS2 demonstrated robust photothermal effects, significantly reducing bacterial viability of both Escherichia coli and Staphylococcus aureus. The sustained release of rutin synergistically enhances photothermal antibacterial efficacy while accelerating fibroblast proliferation, thereby facilitating skin wound healing. This dual-functional mechanism resolves the critical trade-off between rapid bacterial eradication and long-term tissue regeneration, offering a translational therapeutic strategy for wound repair.
研究领域:
生物材料
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纳米医学
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伤口修复
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光热治疗
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抗菌治疗
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 36
·5274-5280
·
IF 1.9
Yurong Tang,Shuguang Yan,Hang Ye,Fenglan Zheng,Liu Feng,Feng Liu,Xulong Li
The design of quantum dots as a new sensing platform is very important for developing high-sensitivity fluorescent sensors. However, achieving a new strategy is still a great challenge. Here, a new strategy for fabricating water-dispersible thiol malic acid modified ZnSe quantum dots (TMA-ZnSe QDs) is presented in aqueous solution. The characteristics of the modified TMA-ZnSe QDs and the interactions between TMA-ZnSe QDs, Al3+ and rutin were investigated by atomic force microscopy, energy dispersive X-ray spectroscopy, fluorescence spectrometry, resonance Rayleigh scattering, UV-vis absorption spectroscopy and Fourier transform infrared spectroscopy. A new high-sensitivity fluorescent sensor based on tuning the aggregation/disaggregation behavior of TMA-ZnSe QDs by structure-switching for the detection of rutin was developed. The results confirmed that the detection sensitivity was greatly improved by the aggregation of TMA-ZnSe QDs with Al3+ because rutin effectively quenched the FL of TMA-ZnSe QDs–Al3+via the photoinduced electron transfer process. Under optimum conditions, the decrease in the FL intensity was linear to the rutin concentration from 0.02 μg mL−1 to 9 μg mL−1 and the detection limit for rutin was 0.02 μg mL−1. The proposed method offered a new possibility for rutin detection in real samples owing to its low cost, simplicity, high sensitivity and good stability. The present work provides a new strategy for improving sensitivity by tuning the aggregation/disaggregation behavior of TMA-ZnSe QDs.
研究领域:
分析化学
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纳米生物技术
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分子诊断
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药物分析
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荧光传感
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DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Deqing Wang,Pu Zheng,Pengcheng Chen,Dan Wu
Deep eutectic solvents (DESs), considered to be “green media”, have recently been employed in multiple applications including biotransformations. Here, a DES was used in the production of isoquercitrin and l-rhamnose from rutin by enzymatic transformation with recombinant α-l-rhamnosidase from Aspergillus niger. A solution synthesized from choline chloride and urea (ChCl-U) performed best in the conversion of rutin among 24 kinds of DESs. When 130 g/L rutin was used as the substrate, the conversion rate was 100% and the productivity of l-rhamnose and isoquercitrin was 208.68 mM/h under optimized conditions in ChCl-U solution, which was 1.12 times higher than that in buffer (186.92 mM/h). The recycling of ChCl-U solution was also investigated by aqueous two-phase system extraction of l-rhamnose, and ChCl-U/K2HPO4 was screened as the suitable extraction solvent. When the ChCl-U content was 34%, the extraction efficiency of l-rhamnose was 79.06% with a purity and primary crystallization yield of 96.28 and 66.49%, respectively. After five cycles of ChCl-U solution, the average enzymatic conversion rate of recycling was 95.63% with an average purity of 94.22% of the isoquercitrin prepared. After 10 cycles of reuse, the residual conversion rate was 61.14%. The results indicate that the DES has obvious advantages in biocatalytic processes involving flavonoid conversion.
研究领域:
生物化学
·
绿色化学
·
酶工程
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DOI
高影响因子
TALANTA
·
2016
·
IF 4.035
Shiqiang Cui,Li Li,Yaping Ding,Jiangjiang Zhang,Hua Yang,Yingzi Wang
The glassy carbon electrode (GCE) modified with mesoporous NiCo 2 O 4 -decorated reduced graphene oxide (NiCo 2 O 4 /rGO) was first applied for the electrochemical determination of rutin . The synthesized NiCo 2 O 4 and NiCo 2 O 4 /rGO were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Brunauer-Emmett-Teller (BET) method. The sensor not only showed a satisfactory linear range (0.1–150 μM) and detection limit (0.01 μM) but also exhibited the good anti-interference abilities, low price, high stability, as well as favorable precision and accuracy. The present work is meaningful to expand functionalized graphene composites to sensor fields and promote the development of rutin sensors.
研究领域:
材料科学
·
分析化学
·
药物分析
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DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 57
·32740-32746
·
IF 2.936
Ya-feng Jin,Chuang-ye Ge,Xiao-bo Li,Miao Zhang,Guang-ri Xu,Dong-hao Li
Herein, we fabricated a sensitive rutin electrochemical sensor via modifying glassy carbon electrode (GCE) with zeolitic imidazolate framework-8 (ZIF-8) and acetylene black (AB) in the presence of chitosan (CS). The electrochemical activity and experimental parameters of the ZIF-8-AB-CS/GCE sensor were investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimal conditions, the sensor presented a reasonable linear response in the range of 0.1–10 μM with a limit of detection (LOD) as low as 0.004 μM (S/N = 3). The sensor possessed good reproducibility and high stability, and was successfully applied to detect rutin tablet samples with satisfactory results, which was attributed to the synergistic effect between ZIF-8 and AB. Meanwhile, the sensor displayed a potential application for detection of other analytes in real samples. Furthermore, a probable interaction mechanism was proposed to account for the interaction between rutin and the nanocomposite electrode, which was not discussed in previous reports.
研究领域:
分析化学
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材料科学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Guangquan Mo,Qiuxia Li,Liu Zhu,Zhixiong Ruan,Songqing Mei
Herein, hollow and porous nano-dodecahedron embedded with cobalt oxide nanoparticles (HPND-CN) was prepared for the detection of rutin (Rut). HPND-CN was synthesized by the pyrolysis of zeolitic imidazolate frameworks (ZIFs). HPND-CN displayed a uniform rhombic dodecahedral morphology. For the main architecture, the original framework was carbonized after pyrolysis leading to a porous graphitized polyhedron with nitrogen doping. Notably, in the polyhedron, spherical cobalt oxide nanoparticles with more CoO and less Co 2 O 3 were evenly implanted. With embedded oxide nanoparticles, active doping species, polyhedral morphology, and porous graphitized structure, HPND-CN displayed a strong electrocatalysis towards the redox of Rut. A pair of well-defined symmetric peaks for Rut redox was clearly observed. Based on the electrocatalysis of HPND-CN, an efficient Rut sensor was developed with a low detection limit of 1.9 nM and broad detection range from 7.0 nM to 1.0 μM. HPND-CN prepared sensor also exhibited a superb selectivity showing no interference from common inorganic ions, carbohydrates, and amino acids. Besides, excellent repeatability, reproducibility and reliability were observed. Moreover, HPND-CN sensor was successfully applied to the measurement of Rut in health-care tablets with convinced results, indicating a substantial electrochemical sensing platform for the electrocatalysis and detection of macromolecules.
研究领域:
分析化学
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材料科学
·
药物分析
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DOI
高影响因子
危险品化学品经营许可证（带存储）