FOOD CHEMISTRY
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2023
·
IF 8.8
Junmin Ji,Yaxin Zhang,Dan Wang,Yan Wang
The coconut shell-based activated charcoal was decorated by three different electronegativities of surfactants (CTAB, SDS, and Triton X-100) through the impregnation method, and the decorated activated charcoal adsorbents were used for the removal of PAHs from peanut oil, respectively. The influence of surfactant decoration on the adsorption and detoxification effect of coconut shell-based activated charcoal was discussed. The thermodynamic and kinetic behaviors of PAHs adsorption on the surfactant-modified activated charcoal were investigated, and the adsorption mechanism was analyzed in-depth. Notably, the prepared modified coconut shell activated charcoal could not only remove more than 90% of PAHs from the peanut oil but also keep the cytotoxicity of the treated peanut oil low. Meanwhile, the detoxification procedure has little effect on the nutritional quality and flavor of the peanut oil. The results of this fundamental study demonstrate that the low-cost surfactant-modified coconut shell-based activated charcoal was effective and feasible.
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高影响因子
ANALYTICAL LETTERS
·
2022
·
IF 2.267
Zhengjie Hu,Zhiying Chen,Xuwei Chen
Adsorbents for protein enrichment underline the most important foundations in proteomic application. The construction of tunable metal-organic framework (MOF) through rational selection of metal nodes and organic linkers with designed properties has attracted special interest in the field of protein enrichment. In this work, a nickel-based MOF (NiBPDC) composed of biphenyl-based organic ligand and Ni clusters was prepared which exhibits attractive adsorption efficiency and selectivity for hemoglobin (Hb) by taking advantage of the strong metal affinity and π stacking interaction between Hb and MOF. The adsorption maximum capacity of NiBPDC for Hb is 400.2 mg g−1, and the adsorbed Hb is easily recovered by 0.5% (m/v) sodium dodecyl sulfate achieving a recovery of 98.2%. An enrichment factor up to 79.8 for Hb from an extremely 300:1 mass ratio of a human serum albumin/Hb mixture was achieved. Circular dichroism (CD) spectra reveal that the recovered Hb maintained its original structure following the enrichment. The practicability of NiBPDC is confirmed by selective enrichment of Hb from complex biological samples followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.
研究领域:
材料科学
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蛋白质组学
·
分析化学
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DOI
高影响因子
TALANTA
·
2018
·
IF 4.244
Jundong Wang,Siyuan Tan,Qionglin Liang,Shaoai Sun,Qiang Han,Mingyu Ding
The isolation and depletion of highly-abundant proteins and peptides is of vital importance for the screening and monitoring of biomarkers which are low stoichiometry in proteomics and protein-based biotechnologies. Here we have designed and synthesized a series of magnetic microspheres MnFe 2 O 4 @SiO 2 @ Lanthanide Oxides (La= Ce, Sm, Er, Tm, Yb) whose surface were functionalized by lanthanide oxides that could successfully isolate bovine hemoglobin (BHb), and MnFe 2 O 4 @SiO 2 @Er 2 O 3 microspheres were found to be most efficient in separating BHb with excellent selectivity and a binding capacity as high as 238.2 mg/g. The resulting microspheres demonstrated the favorable performance for isolation of BHb from bovine serum as confirmed by SDS-PAGE, which would open a promising strategy in the pretreatment of proteomic analysis.
研究领域:
材料科学
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生物化学
·
蛋白质组学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Yanan Wang,Xuezhi Tian,Zehua Zhang,Meiling Tian,Fusheng Zhang
The aim of this study is to enhance the efficiency of enzymes, reduce operational costs, and increase production output. Experimental research shows that ultrasound positively impacted the activity of α-amylase and glucoamylase, with the peak α-amylase activity observed at 30 W for 5 min and the highest glucoamylase activity at 60 W for 3 min. Ultrasonication extended the optimal temperature of both enzymes, but had no effect on the optimal pH. According to the kinetic experiments, ultrasonic treatment could improve the catalytic efficiency of α-amylase and glucoamylase. Furthermore, the SDS-PAGE results revealed that ultrasound treatment did not alter the molecular weights of the enzymes and their primary structures; The circular dichroism and fluorescence spectroscopy analysis indicated the secondary structural components, especially the α-sheet and the tryptophan fluorescence intensity, were notably affected by ultrasound. The changes in the secondary and tertiary structure of α-amylase and glucoamylase suggest that ultrasonic treatment may enhance their activities. Our findings provide a theoretical basis for the efficient utilization of α-amylase and glucoamylase through understanding the enzyme structure changes after ultrasound.
研究领域:
生物化学
·
酶学
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超声技术
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食品科学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Shouning Yang,Yanmin Zhang,Sijia Lu,Lihong Liu,Lin Yang,Yuming Guo,Shaoning Yu,Huayan Yang
One of the main diseases contributing to human death are malignant tumors. Phototherapy is a promising approach for cancer therapy, and functional nanoparticles with targeting ligands are commonly used to improve the therapeutic efficiency. However, recent studies have shown that nanoparticles in contact with a biological fluid can rapidly form a “protein corona” on their surface, which will remarkably decrease the targeting ability. Here, we describe the preparation of hybrid nanomaterials with Bi 2 S 3 nanorods as the core, and fluorescein-isothiocyanate and folic acid-modified human serum albumin (HSA-FITC-FA) as the shell. By using fluorescent binding label (FITC) and imaging techniques, we discovered the image of the cell lysosomes, indicating that the photothermal therapy agent was predominantly targeted to and accumulated in lysosomes. Combined with photothermal therapy agent (Bi 2 S 3 nanorods) and targeting ligand (FA), the obtained product shows enhanced photothermal therapy under near-infrared region laser irradiation. Additionally, SDS-PAGE shows that the modified HSA shell could remarkably reduce the reabsorption of protein corona from blood serum, minimized the adverse effect of protein corona on targetability. Taken together, the results indicate that our strategy has the potential for preparing efficient photothermal nanomaterials with image-guided subcellular organelle-targeting cancer cell ablation ability.
研究领域:
纳米技术
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癌症治疗
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生物成像
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材料科学
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生物医学工程
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 11
·2893-2901
·
IF 4.157
Zhang Mengmei,Wang Hao,Bhandari Ramesh,Pan Qiuhui,Sun Fenyong
Selective separation and enrichment of phosphoproteins possess the distinct clinical and biological importance in the diagnosis, treatment, and management of several fatal human diseases. In this study, a facile synthesis of titanium(IV) ion–immobilized arsenate-modified poly(glycidyl methacrylate) microparticles (denoted as Ti 4+ -arsenate-PGMA-MPs) was developed for the efficient enrichment of intact phosphoproteins found in biologically complex protein samples. By virtue of the strong interaction between the titanium ions immobilized on the surface of Ti 4+ -arsenate-PGMA-MPs and phosphate groups of phosphoproteins, Ti 4+ -arsenate-PGMA-MPs had a high saturated adsorption capacity for phosphoproteins (901 mg/g for β-casein), which was much higher than that of non-phosphoproteins (73.5 mg/g for BSA). Ti 4+ -arsenate-PGMA-MPs were characterized by SEM, TEM, and FT-IR, and the average particle diameter was about 2.5 μm with good dispersibility. Besides, the application of Ti 4+ -arsenate-PGMA-MPs in real biological samples was investigated by SDS-PAGE analysis, and the results showed that Ti 4+ -arsenate-PGMA-MPs were able to enrich phosphoproteins efficiently. Graphical abstract
研究领域:
生物化学
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蛋白质组学
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材料科学
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PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 7
·1656
·
IF 4.2
Qian Zhang,Linlin Hu,Jianyu Yang,Pengfei Guo,Jinhong Wang,Weifen Zhang
Immobilized metal ion affinity chromatography (IMAC) adsorbents generally have excellent affinity for histidine-rich proteins. However, the leaching of metal ions from the adsorbent usually affects its adsorption performance, which greatly affects the reusable performance of the adsorbent, resulting in many limitations in practical applications. Herein, a novel IMAC adsorbent, i.e., Cu(II)-loaded polydopamine-coated urchin-like titanate microspheres (Cu-PDA-UTMS), was prepared via metal coordination to make Cu ions uniformly decorate polydopamine-coated titanate microspheres. The as-synthesized microspheres exhibit an urchin-like structure, providing more binding sites for hemoglobin. Cu-PDA-UTMS exhibit favorable selectivity for hemoglobin adsorption and have a desirable adsorption capacity towards hemoglobin up to 2704.6 mg g−1. Using 0.1% CTAB as eluent, the adsorbed hemoglobin was easily eluted with a recovery rate of 86.8%. In addition, Cu-PDA-UTMS shows good reusability up to six cycles. In the end, the adsorption properties by Cu-PDA-UTMS towards hemoglobin from human blood samples were analyzed by SDS-PAGE. The results showed that Cu-PDA-UTMS are a high-performance IMAC adsorbent for hemoglobin separation, which provides a new method for the effective separation and purification of hemoglobin from complex biological samples.
研究领域:
生物化学
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材料科学
·
生物技术
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Feilong Wei,Jianyin Miao,Huiyou Tan,Runqing Feng,Qianwang Zheng,Yong Cao,Yaqi Lan
In this study, the influence of crystal morphology and cooling temperature on the oxidative stability of oleogel-structured perilla oil emulsion was explored. The formation and distribution of the internal crystals were confirmed by differential scanning calorimetry and polarized light microscopy. All beeswax (BW) crystals existed in the internal phase while stearic acid (SA) crystals appeared in the aqueous phase as well. As the crystal grew, the HPMC/SDS emulsified layers were pierced, leading to release of the SA crystals into the aqueous phase with partial droplet flocculation. As a result, the physical and oxidative stability of the BW-structured emulsion was significantly better than that of SA-structured emulsion. The crystallinity of the emulsion increased with higher gelator concentration. The denser crystal network were more effective preventing the penetration of pro-oxidants into the internal phase, resulting in the stronger the oxidation stability. Processing methods with different cooling temperature affected more of the properties of bulk oleogels rather than the oleogel-structured emulsion for long-term storage. Overall, choosing a gelling agent that forms relatively small crystals and preparing an oleogel-structured emulsion with appropriate crystallinity can effectively improve the oxidative stability of structured emulsion with longer shelf life.
研究领域:
食品科学
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化学工程
·
材料科学
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高影响因子
JOURNAL OF FOOD ENGINEERING
·
2018
·
IF 3.197
Tiantian Wu,Yuan Li,Ni Shen,Chunhong Yuan,Yaqin Hu
The objective of this study was to study the effect of calcium ions on the binding of lysozyme to chitosan (CS)/sodium alginate (SA) complexes (CS-SA-Ps), in order to develop a complex system for lysozyme delivery which might be of practical interest in the field of food preservatives . Result showed that with the increase of calcium ions (Ca 2+ ) concentration, the value of Z-Ave decreased while the value of ζ-potential and LE increased, where the addition of 5.0 mM Ca 2+ contributed to uniform distribution of the complexes. Sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that there was no significant difference of molecular integrity when lysozyme was released from CS-SA-Ps. The exothermic interaction between lysozyme and SA were proved by thermodynamic measurements, in which the binding stoichiometry showed a slight decrease in the presence of calcium chloride. Moreover, the release rate of lysozyme from CS-SA-Ps could be slowed by the increase of Ca 2+ addition concentration.
研究领域:
食品科学
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材料科学
·
生物化学
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高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2024
·
IF 10.8
Qiong Li,Shuaishuai Ma,Qiuyue Ge,Xinyuan Wu,Yu Liu,Xiang Tu,Liwu Zhang,Hongbo Fu
Significant discrepancies persist between field observations and model simulations regarding the strength of marine-derived HONO sources, underscoring the urgency to resolve unidentified HONO sources. In this study, sodium dodecyl sulfate (SDS) was chosen as a proxy for marine surfactants to investigate its impact on aqueous nitrate photolysis for the first time. Remarkable increases in HONO and NO2 production rates by factors of 3.3 and 5.6, respectively, along with a 1.9-fold rise in NO2– concentration, were observed at a very low SDS concentration of 0.01 mM, strongly illustrating the promoting effect on nitrate photolysis. Furthermore, at an SDS concentration of 2 mM, intriguingly aligned with the critical micelle concentration, there was an additional 41.7% increase in HONO production rates. Vertically resolved Raman measurements indicated that SDS anions at the aqueous-air interface attracted NO3– closer to the aqueous surfaces, increasing the amount of incompletely solvated surface nitrate. Importantly, the anionic surfactant exhibited a greater promoting effect on HONO production compared to other typical nitrate photochemistry systems with the addition of a marine dissolved organic matter proxy, halogen, photosensitizer, or OH scavenger. These findings offer new insights into marine-derived HONO sources and should be considered in model simulations concerning the budgets of NOx, OH, and O3.
研究领域:
大气化学
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环境科学
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海洋化学
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PubMed
高影响因子
NANOTECHNOLOGY
·
2024
·Vol. 36
·期 6
·065101
·
IF 2.9
Xu Chen,Jian Yue,Xiongjun Xu,Jiajun Chen,Xuechan Huang,Yukai Huang,Yang Yang,Feng Li,Tianwang Li
Selenium-based nanoparticles exhibit antiviral activity by directly modulating immune function. Despite recent promising developments in utilizing selenium nanoparticles (Se NPs) against viral infections, the impact of surface ligand charge on the conformation and interaction with viral proteins, as well as the effectiveness of Se NPs in anti-Herpes simplex virus 1 (HSV-1) infection remains unexplored. In this study, three types of selenium nanoparticles (CTAB-Se, PVP-Se, SDS-Se) with distinct surface charges were synthesized by modifying the surface ligands. We found that apart from differences in surface charge, the size, morphology, and crystal structure of the three types of Se NPs were similar. Notably, although the lipophilicity and cellular uptake of SDS-Se with a negative charge were lower compared to positively charged CTAB-Se and neutrally charged PVP-Se, SDS-Se exhibited the strongest protein binding force during interaction with HSV-1. Consequently, SDS-Se demonstrated the most potent anti-HSV-1 activity and safeguarded normal cells from damage. The mechanistic investigation further revealed that SDS-Se NPs effectively inhibited the proliferation and assembly of HSV-1 by powerfully suppressing the key genes and proteins of HSV-1 at various stages of viral development. Hence, this study highlights the significant role of surface ligand engineering in the antiviral activity of Se NPs, presenting a viable approach for synthesizing Se NPs with tailored antiviral properties by modulating surface charge. This method holds promise for advancing research on the antiviral capabilities of Se NPs.
研究领域:
纳米技术
·
病毒学
·
材料科学
·
免疫学
·
药学
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Min Qiao,Meng-Meng Wang,Ming-Li Chen,Jian-Hua Wang
Porous polymeric microspheres , poly(styrene-divinyl benzene, PSDVB)-poly(ethylene glycol monoallyl ether, PEGMAE), termed as PSDVB-PEGMAE, are prepared via double emulsion interfacial polymerization strategy. PSDVB-PEGMAE microspheres exhibit a mean diameter of 2.98 μm, and possess heterogeneous porous structure with a pore volume of 0.354 cm 3 g −1 and a pore size of 34.3 nm. PEGMAE moiety is identified on the external surface of the microspheres, while both PSDVB and PEGMAE moieties are found in the interior pores. The PSDVB-PEGMAE microspheres possess favorable selectivity towards the adsorption of conalbumin (ConA) through hydrogen-bonding and hydrophobic interactions, via surface and inter-pore adsorption. At pH 6, an adsorption capacity of 171.9 mg g −1 is achieved for ConA. The captured ConA may be readily recovered by stripping with a cetane trimethyl ammonium bromide (CTAB) solution (0.1%, m/v). The microspheres are further used for the isolation of ConA from egg white, deriving high purity ConA as demonstrated by SDS-PAGE assay.
研究领域:
材料科学
·
化学工程
·
生物化学
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PubMed
高影响因子
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