Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Fangze Guo,Changqing Yuan,Hailin Huang,Xuyang Deng,Zirui Bian,Danyang Wang,Keke Dou,Li Mei,Qihui Zhou
Nano-hydroxyapatite (nHA) has been widely applied as a tissue-engineering biomaterial and interacted with osteoblasts/stem cells to repair bone defects. In addition, T cells that coexist with osteoblasts/stem cells in the bone modulate the regulation of osteoimmunology by cytokine formation. However, the effects of nHA on T cells and the following regulatory interplay on osteogenic differentiation have been rarely examined. In this work, the physicochemical properties of needle-like nHA are characterized by field emission scanning electron microscopy, zeta potential, Fourier transform-infrared and X-ray diffraction. It is found that as the concentration of nHA increases, the proliferation of T cells gradually increases, and the proportion of apoptotic T cells decreases. The percentage of CD4 + T cells is higher than that of CD8 + T cells under the regulation of needle-like nHA. Furthermore, the supernatant of T cells co-cultured with nHA significantly inhibits the osteogenic differentiation of MC3T3-E1 by downregulating the formation of alkaline phosphatase and calcium nodule compared with the supernatant of nHA. Thus, our findings provide new insight into the nHA-mediated T cell and osteoblast interactions.
研究领域:
生物材料
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免疫学
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组织工程
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骨生物学
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DOI
PubMed
高影响因子
Nature Biomedical Engineering
·
2019
·Vol. 3
·期 4
·306-317
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IF 17.135
Yao Han,Lan Jiang,Li Chushu,Shi Hubing,Brosseau Jean-Philippe,Wang Huanbin,Lu Haojie,Fang Caiyun,Zhang Yao,Liang Lunxi,Zhou Xiaolin,Wang Chaojun,Xue Yu,Cui Yun,Xu Jie
Checkpoint blockade therapy targeting the programmed-death ligand 1 (PD-L1) and its receptor programmed cell death 1 promotes T-cell-mediated immunosurveillance against tumours, and has been associated with marked clinical benefit in cancer patients. Antibodies against PD-L1 function by blocking PD-L1 on the cell surface, but intracellular storage of PD-L1 and its active redistribution to the cell membrane can minimize the therapeutic benefits, which highlights the importance of targeting PD-L1 throughout the whole cell. Here, we show that PD-L1 is palmitoylated in its cytoplasmic domain, and that this lipid modification stabilizes PD-L1 by blocking its ubiquitination, consequently suppressing PD-L1 degradation by lysosomes. We identified palmitoyltransferase ZDHHC3 (DHHC3) as the main acyltransferase required for the palmitoylation of PD-L1, and show that the inhibition of PD-L1 palmitoylation via 2-bromopalmitate, or the silencing of DHHC3, activates antitumour immunity in vitro and in mice bearing MC38 tumour cells. We also designed a competitive inhibitor of PD-L1 palmitoylation that decreases PD-L1 expression in tumour cells to enhance T-cell immunity against the tumours. These findings suggest new strategies for overcoming PD-L1-mediated immune evasion in cancer.
研究领域:
癌症免疫学
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分子生物学
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免疫疗法
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
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IF 3.131
Xia Li,Xiao-Juan Nie,Yu-Nuo Zhu,Wen-Chao Ye,Yu-Lin Jiang,Shi-Long Su,Bi-Ting Yan
In this paper, a new β-cyclodextrin polymer (β-CDBEP) was successfully prepared by derivatizing with 1H-benzotriazole followed by cross-linking with epichlorohydrin . The adsorption behaviour of β-CDBEP for anionic Eriochrome Black T (EBT) was studied by batch experiments. The results showed that the highest adsorption capacity was obtained in acidic medium (pH = 5), and the pseudo-second-order model fitted well with the experimental data indicating the rate-determining step mainly determined by chemisorption . The adsorption equilibrium data followed better the Freundlich isotherm model with multi-layered adsorption compared to the Langmuir isotherm model. Thermodynamic parameters indicated that the adsorption of EBT onto β-CDBEP was an endothermic and spontaneous process, and a favorable adsorption happened at a relatively higher temperature. The combined electrostatic interactions , inclusion complexation, π-π interactions and intra-particle diffusion contributed to the adsorption of EBT onto β-CDBEP.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
Foods
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2025
·Vol. 14
·期 24
·4323
·
IF 5.1
Yazhen Liu,Jiaqi Huang,Ji Yu,Luxin Fu,Ronglong Huang,Jing Liu,Bailin Deng,You-Bao Zhong,Duanyong Liu,Haimei Zhao
(1) Background: Ulcerative colitis (UC) is a persistent inflammatory condition of the intestine, characterized by dysregulated T cell-mediated immune responses. Curcumin (CUR), a common food additive and health supplement, is noted for possessing anti-inflammatory and immunomodulatory properties. Nevertheless, the molecular mechanisms underlying its therapeutic effects remain incompletely elucidated. This research aims to investigate the therapeutic mechanisms of CUR in UC, focusing on its role in restoring T cell homeostasis by modulating TIGIT and Neuropilin-1 (NRP1). (2) Methods: We employed a DSS-induced murine colitis model, combined with network pharmacology, molecular docking, protein–protein interaction docking, molecular dynamics simulations, andinvitroassays with Jurkat T cells. (3) Results: CUR markedly ameliorated clinical manifestations and histopathology in DSS-treated mice, restoring the balance of T cell and memory T cell subsets. Computational predictions and experimental validation showed that CUR downregulated TIGIT and NRP1 expression in inflamed colonic tissue and directly inhibited their expression in activated T cellsinvitro. (4) Conclusions: This study reveals a novel immunoregulatory mechanism of this natural compound. These findings suggest CUR modulates TIGIT/NRP1 to inhibit excessive T cell activation and restore immune homeostasis in UC.
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DOI
PubMed
高影响因子
Scientific Reports
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2023
·Vol. 13
·期 1
·1-13
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IF 4.6
Grayson Jason M.,Short Samantha M.,Lee C. Jiah,Park Nuri,Marsac Caitlyn,Sette Alessandro,Lindestam Arlehamn Cecillia S.,Leng Xiaoyan I.,Lockhart Samuel N.,Craft Suzanne
Studies over the last 100 years have suggested a link between inflammation, infectious disease, and Alzheimer’s Disease (AD). Understanding how the immune system changes during the development of AD may facilitate new treatments. Here, we studied an aging cohort who had been assessed for AD pathology with amyloid positron emission tomography and cognitive testing, and conducted high dimensional flow cytometry on peripheral blood mononuclear and cerebrospinal fluid cells. Participants were assigned a classification of being amyloid negative cognitively normal, amyloid positive cognitively normal (APCN), or amyloid positive mild cognitive impairment (APMCI), an early stage of AD. We observed major alterations in the peripheral innate immune system including increased myeloid and plasmacytoid dendritic cells in the blood of APMCI participants. When the adaptive immune system was examined, amyloid positive participants, regardless of cognitive status, had increased CD3 + T cells. Further analyses of CD4 + and CD8 + T cells revealed that APMCI participants had an increase in more differentiated phenotype T cells, such as effector memory and effector memory CD45RA expressing (TEMRA), compared to those with normal cognition. When T cell function was measured, we observed that T cells from APCN participants had increased IFNγ + GzB - producing cells compared to the other participants. In contrast, we demonstrate that APMCI participants had a major increase in T cells that lacked cytokine production following restimulation and expressed increased levels of PD-1 and Tox, suggesting these are exhausted cells. Rejuvenation of these cells may provide a potential treatment for AD.
研究领域:
神经生物学
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免疫学
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老年医学
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DOI
高影响因子
BIOMACROMOLECULES
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2025
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IF 5.4
Wenqian Chen,Zi Ye,Lixiong Gao,Yuhang Jiang,Yue Wei,Xuejun Chen,Liqin Li,Tengfei Mao,Zhaohui Li
The corneal epithelium, the outermost barrier of the eye, is particularly susceptible to UV-induced photodamage. Owing to the limited protection provided by physical defense strategies, there is a critical need for effective UV-protective ocular medications. In this study, novel fluorescent copolymers were synthesized and evaluated for their anti-UV properties in human corneal epithelial (HCE-T) cells. These copolymers provided substantial protection to HCE-T cells against damage from various types of UV radiation. The protective effects were attributed to their ability to attenuate oxidative damage caused by UVA radiation and mitigate direct DNA damage induced by UVB and UVC radiation. Mechanistic investigations revealed that their antioxidant and DNA repair activities are mediated through the regulation of the PERK and NER signaling pathways. These findings underscore the potential of these functional polymers as promising ophthalmic agents against broad-spectrum UV-damage.
研究领域:
生物医学
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材料科学
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眼科学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2018
·Vol. 6
·期 15
·6675-6684
·
IF 9.931
Guofeng Gao,Da Tie,Hao Ma,Haijun Yu,Shanshan Shi,Bo Wang,Shengming Xu,Linlin Wang,Yufeng Zhao
In this work, we report a novel design of mixed P2 + T phase NaxCo0.1Mn0.9O2 (0.44 ≤ x ≤ 0.7) with interface rich characteristics for high performance sodium storage, whereby the tunneled T phase offers fast Na ion diffusivity and excellent structural stability, the layered P2 phase contributes to high specific capacity, and the P2 + T phase interface offers additional channels and active sites for charge transfer and excess charge storage. Consequently, the as-prepared NaxCo0.1Mn0.9O2 (0.44 ≤ x ≤ 0.7) demonstrates an excellent discharge capacity of 219 mA h g−1 at a current rate of 0.1C (1C = 176 mA g−1), and retains 117 mA h g−1 even at a high rate of 5C. This outstanding performance is significantly superior to that of pure T-type Na0.44Co0.1Mn0.9O2 and P2-type Na0.7Co0.1Mn0.9O2, and also outperforms state-of-the-art manganese-based cathodes. The kinetic analysis indicates the drastically improved Na+ diffusion coefficient of the P2 + T phase, which is 6 and 200 times that of the pure T and P phases, respectively. The good reversibility and structural stability is evidenced through an ex situ XRD study.
研究领域:
材料科学
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电化学
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DOI
高影响因子
International Journal of Electrochemical Science
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2023
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IF 1.5
Yue Huang,Xiaojing Si,Mei Han,Chen Bai,Sijie Liu
In this paper, a novel electrochemical sensor based on carboxylated multi-walled carbon nanotubes (C-MWCNTs) and chromium black T (EBT) composites modified electrodes (EBT/C-MWCNTs/GCE) were proposed for the sensitive determination of ethyl maltol (EMA). Cyclic voltammetry and differential pulse voltammetry techniques were used to investigate the performance of EMA on EBT/C-MWCNTs/GCE. The results showed that the peak current of EMA was significantly enhanced by the EBT/C-MWCNTs/GCE compared with the bare glassy carbon electrode. The peak current showed a good linear relationship with the mass concentration of EMA in the range of 10 ∼ 1000 μg/L and the detection limit is 3.3 μg/L. Moreover, the sensor is easy to prepare and shows long term stability, which has great potential application for the determination of EMA in edible oil.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
CRYOBIOLOGY
·
2018
·
IF 2.05
Hao Xu,Wenyue Cao,Liang Huang,Min Xiao,Yang Cao,Lei Zhao,Na Wang,Jianfeng Zhou
Chimeric antigen receptor T (CART) cell therapy has emerged as a potentially curative “drug” for cancer treatment. Cryopreservation of CART cells is necessary for their clinical application. Systematic studies on the effects of cryopreservation on the antitumor function of CART cells are lacking. Therefore, we compared the phenotypes and functions of CART cells that were cryopreserved during ex vivo expansion with those of freshly isolated populations. T cells expressing an anti-B-cell-maturation-antigen (BCMA) chimeric antigen receptor (CAR) were expanded in vitro for 10 days and then cryopreserved. After one month, the cells were resuscitated, and their transduction rates, apoptosis rates and cell subsets were examined via flow cytometry. The results indicated no significant changes in transduction rates or cell subsets, and the survival rate of the resuscitated cells was approximately 90% Furthermore, similar tumoricidal effects and degranulation functions of the resuscitated cells compared with normally cultured cells were verified by calcein release and CD107a assays. A NOD/SCID mouse model was used to estimate the differences in the in vivo antitumor effects of the cryopreserved and normally cultured T cells, but no significant differences were observed. Following co-culture with several target cell types, the cytokines released by the cryopreserved and normally cultured T cells were measured via enzyme-linked immunosorbent assays (ELISAs). The results revealed that the release of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) was significantly decreased. These data demonstrated that with the exception of a decrease in cytokine release , the cryopreserved CART cells retained their antitumor functions.
研究领域:
肿瘤学
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免疫治疗
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细胞生物学
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DOI
PubMed
高影响因子
Journal of Inflammation Research
·
2025
·
IF 4.1
Meng-Ke Song,Meng-Qi Wang,Yu-Qing Ruan,Can Cui,Wen-Gang Chen,Opeyemi Joshua Olatunji,Yan Li,Jian Zuo
Introduction: Qing-Luo-Yin (QLY) is an anti-rheumatic herbal formula potentially activating PPARγ. The study investigated if and how this property contributes to its anti-angiogenesis effects.Methods: Adjuvant-induced arthritis (AIA) rats were orally treated by QLY or rosiglitazone (a PPARγ agonist), and their monocytes and lymphocytes were co-cultured reciprocally in vitro with different sera. Healthy littermates received blood transfusion from QLY-treated or AIA model rats. Two days ahead of sacrifice, a matrigel plug was implanted in the recipients. AIA serum-incubated THP-1 monocytes and Jurkat T cells were treated by a mixture comprised sinomenine, berberine and palmatine. Jurkat T cells-related media and T0070907 were used to stimulate human umbilical vein endothelial cells (HUVECs).Results: QLY and rosiglitazone similarly alleviated joint injuries, synovial angiogenesis and metabolic disorders in AIA rats. Although QLY impaired inflammatory phenotype of AIA rat monocytes in vivo, it cannot be achieved or sustained in vitro. Lymphocytes of QLY-treated AIA rats had a weak inflammatory phenotype and failed to induce inflammatory polarization of monocytes. AIA blood-induced angiogenesis in the matrigel plug, a phenomenon invisible in QLY group. QLY therapy inhibited pathogenic functions of AIA rats’ lymphocytes, shown by changes of cytokines network in the recipients’ joints, where these cells accumulated. The related compounds affected secretion of Jurkat T cells cultured in AIA serum, which lost the potential in activating HUVECs. This effect disappeared in presence of T0070907, a PPARγ inhibitor.Conclusion: Angiogenesis amelioration during QLY therapy is an indirect result from PPARγ activation-caused functional changes of T cells.
研究领域:
药理学
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免疫学
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风湿病学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-13
·
IF 9.429
Luo Zhenyu,Luo Lihua,Lu Yichao,Zhu Chunqi,Qin Bing,Jiang Mengshi,Li Xiang,Shi Yingying,Zhang Junlei,Liu Yu,Shan Xinyu,Yin Hang,Guan Guannan,Du Yongzhong,Cheng Ningtao,You Jian
Adoptive cell therapy (ACT) was one of the most promising anti-tumor modalities that has been confirmed to be especially effective in treating hematological malignancies. However, the clinical efficacy of ACT on solid tumor was greatly hindered by the insufficient tumor-infiltration of cytotoxic CD8 + T cells. Herein, we constructed a nanoplatform termed dual-binding magnetic nanoparticles (DBMN) that comprised PEG-maleimide (Mal), hyaluronic acid (HA) and Fe3O4 for adoptive T cell-modification and ACT-sensitization. After a simple co-incubation, DBMN was anchored onto the cell membrane (Primary linking) via Michael addition reaction between the Mal and the sulfhydryl groups on the surface of T cells, generating magnetized T cells (DBMN-T). Directed by external magnetic field and in-structure Fe3O4, DBMN-T was recruited to solid tumor where HA bond with the highly expressed CD44 on tumor cells (Secondary Linking), facilitating the recognition and effector-killing of tumor cells. Bridging adoptive T cells with host tumor cells, our DBMN effectively boosted the anti-solid tumor efficacy of ACT in a mouse model and simultaneously reduced toxic side effects.
研究领域:
癌症研究
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纳米技术
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免疫疗法
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Zhenguo Zhang,Hongshuai Wei,Yunqing Zhi,Congcong Zhang,Min Jia,Lixia Lu,Kaijing Wang,Jun Zhou,Xin Du
Mucosal-associated invariant T (MAIT) cells exhibit significant potential in the assessment of tumor development and immunotherapy. However, there is currently no convenient and efficient method to analyze the quantitative changes of MAIT cells during cancer development and treatment, which has not been extensively studied. Here, we report an electrochemical biosensor designed to efficiently monitor MAIT cells in peripheral blood by simultaneously recognizing Vα7.2 and CD161 on MAIT cells. Natural red blood cell membrane, tetrahedral DNA nanostructure, and modified nanometal framework are selected as antifouling coating, antibody scaffold, and electrochemical probe, respectively. Owing to the synergistic effects of these materials, the biosensor achieves robust antifouling ability while maintaining excellent detection performance using rapid differential pulse voltammetry. We show a decrease in the number of MAIT cells in peripheral blood associated with aging and the development of mucosa-associated tumors. Our research has prospects in assessing the degree of malignancy of tumors, distinguishing immunotherapy responses in patients, reducing costs, and promoting the transformation of electrochemical sensing technology into clinical settings.
研究领域:
医学
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生物技术
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肿瘤学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）