LUMINESCENCE
·
2025
·Vol. 40
·期 7
·e70257
·
IF 3
Yingling Wu,Longxiao Liu,Xutao Xu,Yongmin Duan,Haojie Fu,Wenyu Wang,Zhijun Chen,Jinquan Liu
Dopamine (DA) is a critical neurotransmitter linked to various neuropsychiatric disorders, emphasizing the need for precise detection in clinical settings. Herein, a novel colorimetric method for detecting DA was developed using gold nanocluster@covalent organic frameworks (AuNCs@COF) with peroxidase-like activity. The composite was created by integrating ultrasmall, uniformly distributed gold nanoclusters (AuNCs) into a COF matrix, retaining COF' advantages while enhancing catalytic activity and stability. AuNCs@COF catalyzed the oxidation of the colorless substrate TMB in the presence of H 2 O 2 , producing a blue product (oxTMB) with an absorbance peak at 652 nm. DA inhibited this catalytic activity, enabling visual and quantitative detection. The method demonstrated sensitivity (detection limit: 14 μmol/L) and a wide dynamic range (50–500 μmol/L), with excellent selectivity, reproducibility, and successful application in detecting DA in human urine samples (recovery rates: 93.7%–111.5%, RSD < 2.5%). This study underscores the potential of AuNCs@COF as a robust nanozyme for rapid, cost-effective, and user-friendly DA detection, holding promise for clinical diagnostics and point-of-care applications.
研究领域:
纳米技术
·
生物分析化学
·
酶模拟物
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生物传感器
·
神经化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ziqi Ye,Changshuai Shang,Yang Wang,Zhifan Liu,Chaihong Gong,Xiaoxiao Ge,Weiying Zhang,Jing Li,Zhenzhong Guo
Utilizing the peroxidase (POD) activity of nanozymes to construct the colorimetric sensors is a popular strategy for assessing the antioxidant capacity of biological molecules, but the detection sensitivity is often compromised by their low enzymatic activity. Pt-based nanozymes, favored by their regulated nanostructures, well provide numerous binding sites for H 2 O 2 , and exhibit enhanced POD performance. Here, we utilized a simple hydrothermal method to synthesize PtTeNi nanomaterials featured with the ultrathin sheets for detecting biological antioxidant (ascorbic acid (AA) as the model). With the TMB-H 2 O 2 system as the colorimetric substrate, PtTeNi nanomaterial exhibited superior POD-like activity with a higher Vmax (5.02 × 10 −4 mM s −1 ) and lower Km (3.14 mM)) in contrast to natural HRP. AA significantly inhibited the colorimetric reaction of PtTeNi-TMB-H 2 O 2 system via competitively binding OH generated by PtTeNi-mediated H 2 O 2 catalysis and further blocking oxTMB formation. Inspired by this fact, the PtTeNi-TMB-H 2 O 2 colorimetric sensor successfully realized highly sensitive and selective detection of AA with a good linear range from 5 μM to 500 μM and the detection limit of 2.94 μM. For analyzing the total antioxidant capability and AA contents in actual samples (commercial drinks and vitamin C tablets), the PtTeNi-based colormetric method displayed comparable results with the commercial total antioxidant capacity assay kit, with the advantages of easy operation, convenient storage and high stability. This work fully affirms the excellent enzyme-like performance of Pt-based nanomaterial and confirms the feasibility of nanozymes-based colorimetric sensing strategy in detecting antioxidant capability of biological molecules.
研究领域:
纳米技术
·
分析化学
·
生物传感器
·
材料科学
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Jiahui Huang,Siyi Wei,Maopu Tu,Weiqi Deng,Le Zhong,Fan Jiang,Zhixin Li,Ray P.S. Han,Xiaoyu Deng,Shaohua Xu,Yingzhou Tao
The measurement of total antioxidant capacity (TAC) is vital for evaluating the nutrient content of foods and beverages. The development of novel mimetic enzyme materials with high catalytic activity has led to the creation of highly sensitive methods for TAC assessment. In this study, we introduce a simple and efficient approach for preparing uniformly distributed Cu 2 O/Cu nanoparticles supported on graphite-phase carbon (g-C@Cu 2 O/Cu NPs). These g-C@Cu 2 O/Cu NPs demonstrate excellent peroxidase-like (POD-like) enzymatic activity and exhibit a strong binding affinity for TMB, with a K m value of 0.24 mM. A colorimetric analytical platform for TAC assessment was developed, utilizing g-C@Cu 2 O/Cu NPs, TMB, and H 2 O 2 as the chromogenic system. The method achieved a limit of detection for ascorbic acid as low as 0.15 μM, with a linear range from 0.5 to 100 μM. Furthermore, this method was successfully applied to evaluate TAC in real samples, highlighting its significant potential for food nutritional analysis.
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Chen Sun,Yutong Ye,Xueting Luo,Wentao Xiang,Cao Li,Bingbing Jiang,Xiaoying Wang,Ziqiang Xu
Total antioxidant capacity (TAC) is a significant indicator for evaluating nutritional value of fruit and beverage industries, in the aspects of their reactive oxygen species (ROS) scavenging activity. The sensitivity and selectivity of the TAC assay based on the peroxidase (POD) assay is restricted by their poor catalytic activity. Herein, a simple and sensitive strategy for assessing TAC in fruits based on light (808 nm) boosted POD like activity of Au 25 nanoclusters (NCs) have been reported. The absorption bands of mercaptohexanoic acid (MHA)-protected Au 25 NCs could be extended into the near-infrared region (∼770 nm) by introducing neutrally charged ligands or 2,2’(ethylene dioxy) diethyl thiol (EDT)) as bi-thiol ligands, which may contribute to more obvious light enhanced POD activity. The most intense absorption at 770 nm can be obtained by the ratio of MHA/EDT=1.7:0.3 (denoted as MEA-17). Since we have confirmed that ROS is involved in the light enhanced POD like activity, the concentration of various antioxidants AA, GSH and L-Cys, can be analyzed by their inhibitory effect to the oxidation of TMB. More importantly, light enhanced POD like activity of MEA-17 have been successfully utilized to analysis TAC value in various fresh fruits. This work not only provide novel direction for the rational development of near-infrared light-enhanced POD-like nanozyme, but also further expands the prospect of the application of Au 25 NCs protected with thiols for TAC analysis.
研究领域:
纳米技术
·
分析化学
·
生物技术
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-7
·
IF 6.232
Huang Qingwei,Zhang Jinli,Li Wei,Fu Yan
Heparin was employed as the stabilizing agent in the synthesis of peroxidase-mimicking Pd nanoparticles. The heparin-capped Pd nanozyme can act as both the signal amplifier and the selective binder of protamine. The most efficient nanozyme with the mean size of 3.5 nm consists of 70.8% metallic Pd 0 and 29.2% Pd 2+ species. Enzyme kinetic studies show that the K m values are 0.036 mM for 3,3′,5,5′-tetramethylbenzidine and 78 mM for H 2 O 2 . Protamine shows strong affinity to the heparin-capped Pd nanozyme, and induces an apparent aggregation of the nanoparticles. This results in a significant inhibition of the peroxidase-mimicking activities. Hence, the oxidation of TMB by H 2 O 2 to a blue product with a maximum absorption at 652 nm is suppressed. Based on this finding, a photometric assay is developed for the determination of protamine. The linear response is in the concentration range 0.02 ~ 0.8 μg mL −1 , and the limit of detection is 0.014 μg mL −1 . This assay presents high selectivity toward other biological substances.
Highly active and selective Pd nanozyme was synthesized through adopting heparin as the capping agent for quantitative determination of protamine.
研究领域:
分析化学
·
纳米技术
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 42
·6858-6864
·
IF 4.776
Wenying Li,Jiayan Wang,Jiacheng Zhu,Yue-Qing Zheng
Nanomaterial-based artificial enzymes (nanozymes), as an emerging generation of artificial enzymes, have received extensive attention in recent years owing to their striking merits. In this study, the obtained Co3O4 nanocrystals exhibited catalase-like activity and could catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2, and a clear absorption peak at 652 nm can be observed. It was verified that the catalytic activity for the oxidation of TMB originated from the oxygen, which is generated by Co3O4 catalyzed H2O2 decomposition. Upon addition of glutathione (GSH), the catalytic ability of the Co3O4 nanocrystals was inhibited. And, the changes of absorbance at 652 nm could be utilized to quantify the concentration of GSH. Under the optimized conditions, the proposed assay showed good linear relationships and a low detection limit towards GSH. Moreover, the Co3O4 nanocrystals had excellent stability and can maintain the catalytic activity for a long time. Thus, a simple, sensitive and selective nanozyme-based biosensor was developed for the colorimetric detection of GSH.
研究领域:
纳米技术
·
分析化学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 7
·1-10
·
IF 6.408
Li Haolin,Sun Huiyuan,Ding Jianwei,Wu Ting,Cai Shuangfei,Wang Chen,Yang Rong
The successful synthesis of ruthenium oxide/reduced graphene oxide (RuO 2 /rGO) heterostructures by one-pot hydrothermal method using graphene oxides and RuCl 3 as precursors is reported. The heterostructures had high peroxidase-like (POD-like) activities, which catalyzes the oxidation of classical peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 to create a blue colored reaction product. The catalytic activity was significantly enhanced by the synergistic effect between RuO 2 nanoparticles and rGO. RuO 2 /rGO had a low K m of 0.068 mM and a high v max of 1.228 × 10 −7 M·s −1 towards TMB in the TMB-H 2 O 2 catalytic oxidation system. In addition, the POD-like activity originating from the electron transfer mechanism was confirmed by cytochrome C (Cyt C) oxidation experiment. A colorimetric method based on RuO 2 /rGO heterostructures was developed with good sensitivity and selectivity for glucose detection with a limit of detection of 3.34 μM and a linear range of 0–1500 μM. The RuO 2 /rGO heterostructures have potential applications in the biomedical areas, such as biosensor and diagnostics. Graphical abstract
研究领域:
生物传感器
·
分析化学
·
材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 12
·期 1
·595-601
·
IF 3.361
Lijuan Chen,Xiang Li,Zezhi Li,Kejian Liu,Jianping Xie
Peroxidases are present widely in microorganisms and plants, and catalyze many reactions. However, the activity of natural peroxidases is susceptible to external conditions. We prepared carbon nanoparticles (CNPs) using an environmentally friendly and simple method. These CNPs were demonstrated to possess intrinsic peroxidase-like activity. CNPs could catalyze the reaction of a peroxidase substrate, 3,3,5,5-tetramethylbenzidine (TMB), in the presence of H2O2 to produce a blue solution at 652 nm. CNPs exhibited higher peroxidase activity than that of other carbon-based nanomaterials. Moreover, CNPs retained their high peroxidase activity after being reused several times. Glutathione (GSH) can change the blue color of oxidized TMB into a colorless hue at 652 nm. Based on this fact, qualitative and quantitative approaches were employed to detect GSH using a colorimetric method. This method showed a broad detection range (2.5–50 μM) with a limit of detection of 0.26 μM. This method was shown to be accurate for GSH detection in a cell culture medium compared with that using a commercial assay kit. Our findings could facilitate application of CNPs in biomedical areas.
研究领域:
纳米技术
·
生物化学
·
分析化学
·
生物医学工程
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Liubing Kong,Ying Gan,Tao Liang,Longjie Zhong,Yuxiang Pan,Dmitry Kirsanov,Andrey Legin,Hao Wan,Ping Wang
As a powerful tool for medical diagnosis and bioanalysis , conventional optical spectrometers are generally expensive, bulky and always require an accompanying data processing device. In this work, we developed a novel smartphone-based CD-spectrometer (SCDS) for high sensitive and ultra-portable colorimetric analysis , with the advantage of cost-effective and simplicity. The distance between the light source and slit, the structure of SCDS and the parameters of camera in the smartphone were all optimized to ensure the best analytical performance. Besides, the SCDS employed HSV color model and utilized the overall intensity calculated by summing V-value of adjacent position for the absorbance measurement. In this way the errors caused by the low resolution of CD-grating can effectively be eliminated to promote the sensitivity of the SCDS. The performance of the SCDS was first validated for colorimetric detection of BSA with a detection limit of 0.0073 mg/mL, which is superior compared to that of the microtiter plate reader (MTPR). Moreover, by combining with 3,3′,5,5′-tetramethylbenzidine-manganese dioxide (TMB-MnO 2 ) nanosheets reaction, a high sensitive and specific system for ascorbic acid detection was established. The SCDS gives a detection range from 0.6250 μM to 40 μM with a detection limit of 0.4946 μM for AA detection. Compared to other studies, the SCDS features wide detection range and very low detection limit with low cost instrument. Therefore, the SCDS will be an ideal and promising colorimetric system for point-of-care (POC) application in food security, disease diagnosis and environmental monitoring.
研究领域:
医学诊断
·
生物分析
·
分析化学
·
即时检测
·
环境监测
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2013
·Vol. 3
·期 1
·1-7
·
IF 2.927
Lu Xiao-feng,Bian Xiu-jie,Li Zhi-cheng,Chao Dan-ming,Wang Ce
Here, we demonstrated a novel method to decorate Cu 9 S 5 nanocrystals on polyaniline (PANI) nanowires using the dopant of mercaptoacetic acid (MAA) in the PANI matrix as the sulfur source under a hydrothermal reaction. TEM images showed that Cu 9 S 5 nanocrystals with a size in the range of 5–20 nm were uniformly formed on the surface of PANI nanowires. Significantly, the as-prepared PANI/Cu 9 S 5 composite nanowires have been proven to be novel peroxidase mimics toward the oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H 2 O 2 . Due to the synergetic effects between polyaniline nanowires and Cu 9 S 5 nanocrystals, the obtained PANI/Cu 9 S 5 composite nanowires exhibit superior catalytic activity over the independent components. This work not only presents a simple and versatile method to decorate semiconductor nanocrystals on the surface of conducting polymer nanostructures, but also provides fundamental guidelines for further investigations into the synergetic effect between conducting polymers and other materials.
研究领域:
材料科学
·
纳米技术
·
催化
·
分析化学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 35
·5121-5126
·
IF 1.9
Li-Juan Wang,Li-Ping Jia,Rong-Na Ma,Wen-Li Jia,Huai-Sheng Wang
A simple colorimetric sensing method for the detection of Hg2+ was developed by combining enzymatic catalysis with DNA-based hybridization chain reactions (HCRs). Firstly, thymine (T)-rich capture DNA (cDNA) was immobilized on a gold electrode via Au–S bonding. In the presence of Hg2+, thymine (T)-rich probe DNA (pDNA) hybridized with cDNA via T–Hg2+–T base pairs. Then the HCRs were realized using pDNA as an initiator and two biotin-labeled hairpin DNAs (H1 and H2) as fuel strands. Finally, numerous avidin-labeled horseradish peroxidase (HRP) enzymes were immobilized on long nicked ds-DNA strands, which can catalyze the H2O2-mediated oxidation of 3,3,5,5-tetramethylbenzidine dihydrochloride hydrate (TMB) to cause a dramatic color change. Under optimal conditions, the absorbance of TMB was linear with the logarithm of Hg2+ concentrations in the range of 1 fM to 1 pM, with a detection limit of 0.33 fM. This strategy exhibited good selectivity and high sensitivity, which might be a potential tool for the practical detection of Hg2+ in environmental monitoring.
研究领域:
分析化学
·
环境科学
·
生物传感器
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2017
·Vol. 5
·期 15
·3757-3764
·
IF 5.256
Jiang-Shan Shen,Jia-Jia Wu,Xiang-Ying Sun,Zhen-Long Wu,Ping Gao,Bin Liu
We found that β-HgS nanocrystals could catalyze the reactions of oxidase substrates such as 3,3′,5,5′-tetramethylbenzidine (TMB) and O-phenylenediamine (OPD) to form coloured and fluorescent products, respectively, but α-HgS nanocrystals could not catalyze these reactions. Further investigation revealed that the predominantly exposed crystal planes may play an important role in determining the catalytic activity. On the basis of these findings, taken together with the reaction of forming a Schiff base between the –NH2 group of OPD and the –CHO group of formaldehyde, a novel, simple, and highly selective photoluminescence (PL) sensing platform for detecting formaldehyde could thereby be successfully developed.
研究领域:
纳米技术
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）