FOOD HYDROCOLLOIDS
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2024
·
IF 11
Jun Li,Fu-Yu Liu,Bao-Lin He,Rui Cui,Hong Wu
Carvacrol has great antibacterial activity against Bacillus cereus , and its action mechanism was comprehensively studied in this work. It could not only control the growth of B. cereus by affecting the integrality of cellular structure and physiological metabolism, but also inhibit the swarming motility, biofilm formation, and toxins production. To increase the stability of carvacrol during the application, the sodium casein (SC)/hydroxypropyl- β -cyclodextrin (HPCD)/carvacrol nanoemulsion was fabricated. The mean particle size and polydispersity index of the nanoemulsions were 113.8 nm and 0.23, respectively, at SC and HPCD concentrations of 2% and 1%. FTIR analysis demonstrated that carvacrol was successfully encapsulated into the nanoemulsions. The MIC and MBC of nanoemulsions against B. cereus were 0.15 and 0.30 mg/mL, respectively, which were lower than those of free carvacrol (0.19 and 0.38 mg/mL). Carvacrol in nanoemulsions showed a slow and sustained release behavior. Furthermore, the nanoemulsions had good stability (particle size <200 nm) after storage at 4 °C and 25 °C for 15 days. Notably, the nanoemulsions were effective in controlling the growth of B. cereus in whole, low-fat, and skim milk and had better antimicrobial activity than free carvacrol. The results indicated that carvacrol nanoemulsions have potential in the antibacterial application of the food industry.
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高影响因子
Food Bioscience
·
2024
·
IF 4.8
Haoyue Ning,Lixin Lu,Yuemei Zhang
To achieve long-term effective protection of food by continuously controlling the release of active substances in an active film, IRMOF-3 was post-synthetic modified with benzoic acid (BA) to obtain BA-IRMOF-3. This modification enhanced the loading capacity of carvacrol (CA) and slowed down its release rate. Research on IRMOF-3 synthesis has found that increasing the proportion of metal nodes can improve its crystallinity. The post-synthesis modification of IRMOF-3 through amide reaction successfully enhanced the binding energy with CA from −5.1 kcal/mol to −6 kcal/mol, resulting in an improved CA loading rate of 28.62% ± 0.15% and delayed release. Furthermore, the BA-IRMOF-3/CA assembly was added to the sodium alginate matrix to prepare the active film BMC@SA, which improved the mechanical and physical properties of the film. The release and antibacterial/antioxidant properties were investigated. The results demonstrated that the active film BMC@SA exhibited significant sustained-release properties and antibacterial/antioxidant activities, reducing the growth of E. coli and S. aureus by 61.95% and 49.33% respectively. In refrigerated pork experiments, the film BMC@SA slowed down the color, weight, pH changes and inhibited microbial growth of fresh pork effectively, and extended the shelf life of pork from 6 d to 12 d, which demonstrated the potential application of active film BMC@SA in food preservation.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yuemei Zhang,Haoyue Ning,Jing Xu,Lixin Lu
This study focused on the encapsulation of modified halloysite nanotubes (AHNT) using sodium alginate (SA) and chitosan (CS) through a layer-by-layer assembly technique. The objective was to develop new composites (CPs) that could control the release of carvacrol (Car) and enhance its stability. The influence of various conditions on the microstructure, chemical properties, and slow-release effects of the CPs was examined by adjusting the pH and concentration of the CS solution. The results indicated that the CPs (CS4.5–1), created with a CS solution concentration of 1 mg/mL at a pH of 4.5, in conjunction with a 1 mg/mL SA solution, demonstrated a superior encapsulation structure and a loading efficiency of 26.33 %. In vitro release experiments confirmed that the CPs exhibited effective slow-release properties for Car. Furthermore, the CS4.5–1 composite provided a physical barrier that resulted in the retention of 94.90 % and 83.61 % of Car after 6 h of exposure to UV light and heat, respectively. Antimicrobial and antioxidant assays indicated that CS4.5–1/Car possessed significant antioxidant properties and effectively inhibited the growth of E. coli and S. aureus . Consequently, the prepared CPs have the potential to enhance the bioactivity of active compounds and may be beneficial for food preservation and other applications.
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高影响因子
Coatings
·
2019
·Vol. 9
·期 9
·583
·
IF 2.33
Qiannan Wang,Hongliang Yu,Bo Tian,Bin Jiang,Jing Xu,Dongmei Li,Zhibiao Feng,Chunhong Liu
The composition and properties of edible coatings (ECs) will significantly influence their effects of food preservation. For the first time, whey protein isolates nanofibers (WPNFs), as a novel material with high hydrophobicity and antioxidant activity, combined with carvacrol (CA) as an antimicrobial agent and glycerol (Gly) as a plasticizer, was used to prepare edible coating (WPNFs-CA/Gly) for preserving fresh-cut Cheddar cheese. The prepared WPNFs and ECs emulsions have been investigated with transmission electron microscopy. Furthermore, the antioxidant activity of ECs emulsions, antimicrobial activity of edible films, and the physical properties of edible films, such as micromorphology, thickness, transparency, and moisture content, have also been evaluated. The weight losses and physical characteristics of both coated and uncoated fresh-cut Cheddar cheese samples have been assessed during storage. The DPPH free radical scavenging rate of WPNFs-CA/Gly emulsion was up to 67.89% and the reducing power was 0.821, which was higher than that of WPI-CA/Gly emulsions. The antimicrobial activity of WPNFs-CA/Gly films was nearly 2.0-fold higher than that of WPNFs/Gly films for the presence of CA. The WPNFs-CA/Gly films had smooth and continuous surfaces, and the transparency reached 49.7% and the moisture content was 26.0%, which was better than that of WPI-CA/Gly films. Furthermore, Cheddar cheese with WPNFs-CA/Gly coatings has shown lower weight losses (15.23%) and better textural properties than those uncoated samples. This in-depth study has provided a valuable and noteworthy approach about the novel edible coating material.Keywords:edible coating;whey protein isolate nanofibrils;antimicrobial activity;antioxidant activity;fresh-cut Cheddar cheese
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Jiyao Zhang,Alkassoumi Hassane Hamadou,Chao Chen,Bin Xu
The application of carvacrol (CAR) in the food industry is hindered by its poor water solubility and instability to environmental stresses. Therefore, the possibility of CAR encapsulation in zein-trehalolipid nanoparticles (Z/TL) via phytic acid-adjusted or HCl-adjusted pH-driven method (PAPD or HAPD) and antisolvent co-precipitation (ASCP) was explored (mass proportion of Z-to-TL = 1:1). The efficacy of loading approaches was also compared based on the physicochemical properties of corresponding encapsulation systems. Regardless the variation of CAR mass, obtained PAPD-Z/TL-CAR always displayed smaller size (91.9–151.8 nm), higher ζ-potential (−45.99∼-28.66 mV), and better encapsulation efficiency (93.69–98.84%). At the optimal mass ratio of Z-to-CAR (5:3), PD-Z/TL entrapped CAR in a more hydrophobic microenvironment than their ASCP counterparts. The self-assembly in spherical Z/TL-CAR including mainly hydrophobic interaction, hydrogen bonding, and electrostatic interactions, were more intense in PD-ones, especially using PA as the acidifier. These structural features are responsible behind the stronger resistance to thermal degradation, lower instability index after centrifugation sedimentation, wider pH range of colloid stabilization, and higher retention of encapsulated carvacrol mass after 21 days storage observed with PAPD-Z/TL-CAR. Moreover, the three encapsulation systems all demonstrated the acceptable aqueous re-dispersibility and controlled-release over a period of 168 h. Overall, PAPD was more appropriate to produce prolamin-amphiphilic emulsifier colloidal particles as prominent carriers for CAR and its analogues.
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Food Packaging and Shelf Life
·
2025
·
IF 10.6
Linna Wang,Hesheng Wang,Limin Guo,Qiyuan Feng,Miaole Zhang,Youwei Yu,Shaoying Zhang
The development of green, edible functional coating materials is crucial for addressing food safety issues. Traditional petroleum-based packaging materials, however, pose significant environmental pollution and resource waste due to their poor biodegradability. In this study, carvacrol (CA) encapsulated in β-cyclodextrin (β-CD) to form microcapsules (CMP) was integrated with a polysaccharide base (CTS/PUL) to create CP-CMP edible coatings. The physicochemical properties and microstructure of these films were evaluated using scanning electron microscopy, and fourier transform infrared spectroscopy. Successful incorporation of CMP into the CP coating via hydrogen bonding was indicated by changes in functional group vibrations, enhancing the barrier and thermal stability of the CP-CMP coating. The antioxidant capabilities of the coating, demonstrated by high DPPH (80.49 %) and ABTS (89.40 %) radical scavenging activities, were remarkable. Furthermore, it significantly inhibited Staphylococcus aureus and Escherichia coli . Applying the developed CP-CMP coating to fresh-cut green peppers extended shelf life from 8 days (control) to 12 days through dual actions: membrane-disrupting antimicrobial activity and integrated antioxidant functions that preserved color and retarded VC and chlorophyll degradation. Thus, the CP-CMP coating exhibits potent antioxidant and antibacterial properties, emerging as a promising alternative to traditional packaging materials. This innovative approach holds significant potential for reducing the environmental impact of white pollution.
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KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
·
2024
·
IF 1.6
Yan Sun,Hai Qu,Xiaohong Niu,Ting Li,Lijuan Wang,Hairui Peng
Type 2 diabetes mellitus (T2DM) is characterized by hyperglycemia and dyslipidemia. Carvacrol (CAR) has demonstrated the potential to mitigate dyslipidemia. This study aims to investigate whether CAR can modulate blood glucose and lipid levels in a T2DM rat model by regulating short-chain fatty acids (SCFAs) and the GPR41/43 pathway. The T2DM rat model was induced by a high-fat diet combined with low-dose streptozocin injection and treated with oral CAR and/or mixed antibiotics. Fasting blood glucose, oral glucose tolerance, and insulin tolerance tests were assessed. Serum lipid parameters, hepatic and renal function indicators, tissue morphology, and SCFAs were measured. In vitro , high glucose (HG)-induced IEC-6 cells were treated with CAR, and optimal CAR concentration was determined. HG-induced IEC-6 cells were treated with SCFAs or/and GPR41/43 agonists. CAR significantly reduced blood lipid and glucose levels, improved tissue damage, and increased SCFA levels in feces and GPR41/43 expression in colonic tissues of T2DM rats. CAR also attenuated HG-induced apoptosis of IEC-6 cells and enhanced GPR41/43 expression. Overall, these findings suggest that CAR alleviates blood lipid and glucose abnormalities in T2DM rats by modulating SCFAs and the GPR41/43 pathway.
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PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 18
·3292
·
IF 3.06
Shiyuan Fang,Qianqian Zhou,Yan Hu,Feng Liu,Jun Mei,Jing Xie
The objective of this research was to explore the antimicrobial activity and mechanism of carvacrol againstVibrio Parahemolyticus,Shewanella putrefaciens,Staphylococcus aureusandPseudomonas fluorescensand evaluate the effect of the addition of carvacrol/β-cyclodextrin emulsions to flaxseed gum (FSG)-sodium alginate (SA) edible films on the preservation of Chinese sea bass (Lateolabrax maculatus) fillets during refrigerated storage. The minimum inhibitory concentration (MIC) of carvacrol againstV. parahemolyticus,S. putrefaciens,S. aureusandP. fluorescenswere 0.5, 0.5, 0.125, and 0.5 mg/mL, respectively. Alkaline phosphatase activity assay, nucleotide and protein leakage, and scanning electron microscope demonstrated that carvacrol damaged the external structure of the tested bacterial cells causing leakage of cytoplasmic components. At the same time, when FSG-SA films containing carvacrol used as coating agents for Chinese sea bass fillets cold storage, FSG-SA films containing 1.0 or 2.0 mg/mL carvacrol could significantly reduce TVB-N content, K-value, the degree of microbial deterioration and maintain quality of sea bass fillets according to organoleptic evaluation results.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Rao Yang,Bingzhen Liu,Fuyou Yu,Hui Li,Yongliang Zhuang
Cellulose paper packaging materials have gained considerable attention as substitutes for petroleum-based plastics owing to their biodegradability , renewability, flexibility, and good mechanical strength. However, high hydrophilicity and the absence of essential antibacterial activity limit their application in food packaging. In this study, a facile and energy-saving method was developed to improve the hydrophobicity of cellulose paper and endow it with a long-acting antibacterial effect by integrating cellulose paper substrate with metal–organic frameworks (MOFs). A dense and homogenous coating of regular hexagonal ZnMOF-74 nanorods was in-situ formed on a paper surface by layer-by-layer assembly followed by low-surface-energy polydimethylsiloxane (PDMS) modification to prepare a superhydrophobic PDMS@(ZnMOF-74) 5 @paper. Excellent anti-fouling, self-cleaning, and antibacterial adhesion performances were obtained for this superhydrophobic paper. In addition, active carvacrol was loaded into the pores of ZnMOF-74 nanorods on PDMS@(ZnMOF-74) 5 @paper to combine antibacterial adhesion together with bactericidal ability, ultimately resulting in a completely “bacteria-free” surface and sustained antibacterial performance. The resultant superhydrophobic papers not only showed overall migration values within the limit of 10 mg/dm 2 but also good stability against various harsh mechanical, environmental, and chemical treatments. This work gave insights into the potential of in-situ -developed MOFs-dopped coating as a functionally modified platform for preparing active superhydrophobic paper-based packaging.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2026
·
IF 8.8
Liuzi Du,Xiaodong Zhai,Xinyang Han,Ke Zhang,Junjun Zhang,Xiaobo Zou,Zhihua Li,Zhikun Yang,Quancai Sun,Ye Peng,Xiaowei Huang
A bilayer TPU film integrating volatile-amine-responsive colorimetric sensing and sustained antibacterial release. • Bromocresol purple/fluorescein enabled dual colorimetric and fluorescent responses over a broad pH range. • Carvacrol/tea tree oil-loaded L/HKUST-1 inhibited both Gram-positive and Gram-negative bacteria. • The film showed reversible responses to NH 3 , DMA, and TMA, while ΔE correlated well with beef TVB-N ( R 2 = 0.9417). • The bilayer film delayed refrigerated beef deterioration by about 4 days and enabled freshness monitoring. A bilayer TPU-based intelligent packaging film integrating colorimetric sensing and antibacterial preservation was developed. The film consisted of a dense cast TPU indicator layer containing a bromocresol purple/fluorescein indicator and an electrospun TPU antibacterial layer loaded with lignin-modified HKUST-1 loaded with carvacrol and tea tree oil. The indicator showed reversible responses to ammonia, dimethylamine, and trimethylamine, with detection limits of 0.37, 0.15, and 0.18 μ M, respectively. Lignin modification improved the aqueous structural retention of HKUST-1, and the electrospun layer exhibited sustained, pH-dependent release of the antibacterial components. Water vapor permeability was further evaluated under both 25 and 4 °C conditions. The film showed antibacterial activity against the four tested bacterial strains ( Escherichia coli , Staphylococcus aureus , Pseudomonas aeruginosa , and Bacillus subtilis ), while its extracts caused no detectable cytotoxicity under the tested conditions. In refrigerated beef, the selected TBF3-T-LH@CT formulation delayed quality deterioration by approximately 4 days and provided visible freshness indication from yellow to orange-red. The color variation correlated well with beef spoilage indicators, supporting visual freshness monitoring. These results demonstrate the potential of the bilayer film for intelligent active food packaging. Download: Download high-res image (212KB) Download: Download full-size image
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yongshi Li,Peng Shan,Fuyou Yu,Hui Li,Lincai Peng
An environmental-friendly composite films containing waste fish scale-derived gelatin (FSG), sodium alginate (SA) and carvacrol loaded ZIF-8 (CV@ZIF-8) nanoparticles were designed and fabricated to develop active food packaging materials capable of sustained antibacterial activity. The microstructure and physicochemical properties of the FSG/SA/CV@ZIF-8 composite films were investigated. The incorporation of CV@ZIF-8 into FSG/SA matrix significantly enhanced the UV-light blocking and the elongation at break, improved water resistance and reduced water vapor permeability, and improved the thermal stability of composite film. The FSG/SA/CV@ZIF-8 film not only exhibited strong antioxidant activity with DPPH radical scavenging rate of 92.35 %, but also showed the satisfactory and long-acting antibacterial ability against E. coli and S. aureus due to slow release of CV from composite film. Strawberry preservation experiment revealed that FSG/SA/CV@ZIF-8 film decelerated the texture deterioration and retarded the growth of spoilage microorganism, resulting in the prolonged shelf-life of 8 days under ambient condition, indicating its promising application prospect in food preservation packaging.
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高影响因子
Food Packaging and Shelf Life
·
2021
·
IF 6.429
Lijing Lu,Yingjie Su,Jing Xu,Haoyue Ning,Xueyu cheng,Lixin Lu
In order to inhibit the spoilage of the packaged food , a gas phase controlled-release low-density polyethylene (LDPE) film containing carvacrol loaded with halloysite nanotubes (HNTs) was prepared and studied in this work. HNTs were etched by 4 mol/L hydrochloric acid to obtain HNT-4M, which increased the carvacrol loading capacity. Then, it was characterized before and after loading with carvacrol by transmission electron microscopy , nitrogen adsorption isotherms, and thermogravimetric analysis . A new LDPE film containing carvacrol adsorbed on HNT-4M was prepared by extrusion, and the physical properties were studied. In addition, the release rate of carvacrol in the film was measured, and the antibacterial and antioxidant properties of the film were evaluated. The results showed that the carvacrol load of HNT increased from 6.7% to 24.2% after acid etching. The diffusion coefficient of carvacrol from the active film containing HNT-4 M/carvacrol was 9.546 × 10 -12 cm 2 ·s -1 , which was decreased by 57.3% compared with the films directly adding carvacrol. Moreover, according to the DPPH radical scavenging assay and micro-atmosphere diffusion antibacterial test, it was verified that carvacrol retained its antioxidant activity and antibacterial activity in the new film.
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