INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Huiyun Zhang,Xinling Li,Weishi Shan,Jingkun Guo,Xuyang Hu,Huaibin Kang
This study aimed to investigate the impact of substituting pork back fat with Pickering emulsion (PE) stabilized by soybean protein isolate-chitosan (SPI-CH) nanoparticles on the quality of pork batters. Cooking loss, rheological properties, water distribution, microstructures, texture, color, and oxidative stability of pork batters were evaluated. The results revealed that replacing fat with PE led to a reduction in fat content and an increase in moisture content, as well as higher L * and b * values ( P < 0.05). Furthermore, the substitution resulted in improved gel performance by enhancing rheological properties and texture, reducing water mobility and cooking loss, as well as effectively inhibiting lipid and protein oxidation in pork batters. However, no significant differences were observed in sensory acceptability. The findings suggest that using SPI-CH nanoparticles as a stabilizer for PE presents significant potential as a nutritionally improved fat alternative in meat products, offering enhanced functional characteristics.
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高影响因子
VETERINARY RESEARCH
·
2025
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IF 3.5
Su Kai,Ren Jing,Zhang Yanan,Yuan Chen,Wang Yawen,Yang Liu,Fu Lizhi,Fan Tingli,Song Qinye
Porcine epidemic diarrhea (PED), a highly contagious intestinal disease caused by PEDV, threatens newborn piglets and causes enormous losses in the swine industry. Intestinal mucosal immunity is vital for defense against PEDV. Chitosan (CS), a mucin-adhesive biodegradable polysaccharide, has emerged as a promising mucosal vaccine delivery vector and antigen adjuvant. To develop a PEDV mucosal vaccine, we prepared S1-CS nanoparticles (NPs) loaded with the S1 domain of the PEDV spike (S) protein. We evaluated their ability to induce mucosal immune responses in mice via different inoculation routes and to confer immune protection in piglets after oral inoculation. The results showed that S1-CS NPs exhibited no cytotoxicity and remained stable in simulated gastric fluid in vitro. Compared with oral administration, intramuscular inoculation of S1-CS NPs induced higher levels of PEDV-specific serum IgG in mice. However, PEDV-specific IgA was detected only in the serum and intestinal lavage fluid of orally vaccinated mice. Oral administration also elicited higher IFN-γ levels than intramuscular injection, suggesting stronger activation of cellular immunity. In piglets, oral S1-CS NPs induced serum neutralizing antibodies and IgA responses in both serum and intestinal mucosa, increased the number of IgA-secreting cells in the intestine, reduced viremia and virus shedding, and improved intestinal villus morphology. These findings indicate that the S1-CS NPs developed in this study represent promising candidates for an oral mucosal PEDV vaccine.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2025
·Vol. 113
·期 12
·e70006
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IF 3.9
Jing Dai,Xuechun Wang,Siyu Li,Shaoqing Chen,Xinye Ni,Min Lin
Severe acute pancreatitis (SAP) is a prevalent gastrointestinal disease with no effective treatment to control inflammation currently. Macrophages, particularly peritoneal macrophages (PMOs), play a pivotal role in SAP inflammation by polarizing into M1 or M2 phenotypes, which exhibit distinct functional properties and cytokine expression profiles. Galectin-9 (Gal-9) modulates macrophage polarization, but its specific effect on PMOs in SAP remains unclear. In this study, hyaluronic acid-chitosan nanoparticles encapsulating Gal-9 (HA-CS-Gal-9 NPs) were developed for delivery. In vitro, HA-CS-Gal-9 NPs enhanced M2 marker expression and suppressed M1 markers in both naive (M0) and LPS-induced M1 macrophages. In vivo, HA-CS-Gal-9 NPs effectively delivered Gal-9, showing effective uptake by PMOs without notable toxicity, resulting in reduced IL-6, and increased IL-10 expression in PMOs. Treatment with these nanoparticles (NPs) decreased systemic pro-inflammatory cytokines, thereby alleviating pancreatitis severity. These findings demonstrate that Gal-9-loaded NPs robustly promoted M2 macrophage polarization, highlighting a promising therapeutic strategy for SAP.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2014
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IF 3.096
Liu Yang,Shiya Gao,Sajid Asghar,Guihua Liu,Jue Song,Xuan Wang,Qineng Ping,Can Zhang,Yanyu Xiao
The aim of this work was to evaluate the potential of polyelectrolyte complex nanoparticles (PENPs) based on hyaluronic acid/chitosan (HA/CS) as carriers for water-insoluble curcuminoid (CUR) and explore in vitro performance against brain glioma cells. PENPs were observed to be affected by the order of addition, mass ratios and initial concentrations of the HA/CS, pH and ionic strength . PENPs remained stable over a temperature range of 5–-55 (C. CUR was successfully encapsulated into the PENPs. CUR-PENPs showed spherical shape with a mean diameter of 207 nm and positive charge of 25.37 mV. High encapsulation efficiency (89.9%) and drug loading (6.5%) was achieved. Drug release studies revealed initial burst release of drug from the PENPs up to 4 h followed by sustained release pattern. DSC thermograms and XRD patterns showed that CUR was encapsulated inside the PENPs in a molecular or amorphous state. Compared with CUR-solution, CUR-PENPs showed stronger dose dependent cytotoxicity against C6 glioma cells and higher performance in uptake efficiency in C6 cells. Cellular uptake of CUR-PENPs was found to be governed by multi-mechanism in C6 cells, involving active endocytosis , macropinocytosis , clathrin-, caveolae-, and CD44-mediated endocytosis . In conclusion, CUR-PENPs might be a promising carrier for therapy of brain gliomas.
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ACS Omega
·
2022
·
IF 4.132
Zhiwei Liu,Na Li,Ping Liu,Zhihui Qin,Tifeng Jiao
Detection of iron ions in aqueous solutions is of significant importance because of their important role in the environment and the human body. Herein, a fluorescent rhodamine B-functionalized chitosan nanoparticles probe is reported for the efficient detection of iron ions. The chitosan nanospheres-rhodamine B (CREN) was prepared by grafting rhodamine B onto the surface of chitosan nanospheres through an amidation reaction. The as-prepared CREN fluorescent probes exhibit high fluorescence intensity under ultraviolet light. When iron ions are added to the CREN solution, they can be coordinated with weak-field ligands such as N and O on the surface of chitosan nanoparticles (CSNP) by a high-spin method. The self-assembly of Fe3+ on the surface of the CREN led to the generation of single electrons and the presence of high paramagnetism, resulting in fluorescence quenching. The quenching effect of Fe3+ on the CREN fluorescent probe can achieve the efficient detection of Fe3+, and the detection limit reaches 10–5 mol/mL. Moreover, this fluorescence quenching effect of Fe3+ on the CREN fluorescent probe is specific, which could not be disturbed by other metal ions and counteranions.
研究领域:
分析化学
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环境科学
·
生物医学
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CARBOHYDRATE POLYMERS
·
2026
·
IF 12.5
Junhao Ye,Ruiquan Hou,Kang Xie,Guangmin Ou,Zhixiang Zhang,Dongmei Cheng
The plant-derived photoactivated fungicide α-Terthienyl (α-T) exerts antimicrobial effects through light-induced reactive oxygen species generation. However, its agricultural application has been constrained by poor targeting capability and low photostability. To address these challenges, we developed a dual-responsive nanoparticle delivery system (α-T NPs) based on a pectin-chitosan polymer network that specifically recognizes the acidic and pectinase-rich microenvironment created by Botrytis cinerea hyphae secretions. Controlled release studies demonstrated that both acidic conditions (pH 5.0) and pectinase exposure substantially promoted α-T release, achieving cumulative release rates of 71.73% and 74.49% respectively over 60 h. The nanoparticle system showed no adverse effects on healthy tomato leaf growth while forming a protective barrier that effectively suppressed fungal infection. Targeted delivery significantly enhanced fungicidal efficacy, with α-T NPs exhibiting an EC₅₀ of 0.419 mg/L compared to 1.943 mg/L for α-T (technical material, TC). Confocal microscopy and quantitative analysis confirmed the enhanced targeting mechanism, revealing substantially stronger fluorescence intensity and higher α-T accumulation specifically on treated fungal hyphae. Additionally, α-T NPs significantly improved photostability, extending the half-life by approximately 1.93-fold under continuous illumination compared to α-T (TC). This work establishes an innovative “pathogen-activated” delivery platform with promising applications in sustainable crop protection.
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PARASITOLOGY RESEARCH
·
2022
·Vol. 121
·期 2
·675-689
·
IF 2.383
Haseeb Muhammad,Huang Jianmei,Lakho Shakeel Ahmed,Yang Zhang,Hasan Muhammad Waqqas,Ehsan Muhammad,Aleem Muhammad Tahir,Memon Muhammad Ali,Ali Haider,Song Xiaokai,Yan Ruofeng,Xu Lixin,Li Xiangrui
Eimeria maxima (E. maxima) are an intracellular apicomplexan protozoan that causes intestinal coccidiosis in chickens. The purpose of this research was to develop a novel delivery approach for recombinant E. maxima (rEm) 14-3-3 antigen to elicit enhanced immunogenic protection using poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NPs) against E. maxima challenge. The morphologies of prepared antigen-loaded NPs (PLGA/CS-rEm14-3-3 NPs) were visualized by a scanning electron microscope. The rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs–immunized chicken-induced changes of serum cytokines, IgY-antibody level, and T-lymphocyte subsets and protective efficacies against E. maxima challenge were evaluated. The results revealed that encapsulated rEm14-3-3 in PLGA and CS NPs presented spherical morphology with a smooth surface. The chickens immunized with only rEm14-3-3 and PLGA/CS-rEm14-3-3 NPs elicited a significant ( p <0.05) higher level of IFN-γ cytokine, stimulated the proportions of CD4 + /CD3 + , CD8 + /CD3 + T-cells, and provoked sera IgY-antibody immune response compared to control groups (PBS, pET-32a, PLGA, and CS). Whereas, PLGA-rEm14-3-3 NP–immunized chicken provoked a higher level of IFN- γ production and IgY-antibody response rather than CS-rEm14-3-3 and bare antigen, relatively. The animal experiment results ratified that PLGA-rEm14-3-3 NP–immunized chicken significantly alleviated the relative body weight gain (%), decreased lesion score, and enhanced oocyst decrease ratio compared to CS-rEm14-3-3 NPs and only rEm14-3-3. The anti-coccidial index of the chicken vaccinated with the PLGA-rEm14-3-3 NPs was (180.1) higher than that of the Cs-rEm14-3-3 NPs (167.4) and bare antigen (165.9). Collectively, our statistics approved that PLGA NPs might be an efficient antigen carrier system (Em14-3-3) to act as a nanosubunit vaccine that can improve protective efficacies in chicken against E. maxima challenge.
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Marine Drugs
·
2022
·Vol. 20
·期 5
·278
·
IF 6.085
Yingqi Mi,Jingjing Zhang,Wenqiang Tan,Qin Miao,Qing Li,Zhanyong Guo
In this study, chitosan nanoparticles (HF-CD NPs) were synthesized by an ionic gelation method using negatively charged carboxymethyl-β-cyclodextrin and positively charged 2-hydroxypropyltrimethyl ammonium chloride chitosan bearing folic acid. The surface morphology of HF-CD NPs was spherical or oval, and they possessed relatively small particle size (192 ± 8 nm) and positive zeta potential (+20 ± 2 mV). Meanwhile, doxorubicin (Dox) was selected as model drug to investigate the prepared nanoparticles’ potential to serve as a drug delivery carrier. The drug loading efficiency of drug-loaded nanoparticles (HF-Dox-CD NPs) was 31.25%. In vitro release profiles showed that Dox release of nanoparticles represented a pH-sensitive sustained and controlled release characteristic. At the same time, the antioxidant activity of nanoparticles was measured, and chitosan nanoparticles possessed good antioxidant activity and could inhibit the lipid peroxidation inside the cell and avoid material infection. Notably, CCK-8 assay testified that the nanoparticles were safe drug carriers and significantly enhanced the antitumor activity of Dox. The nanoparticles possessed good antioxidant activity, pH-sensitive sustained controlled release, enhanced antitumor activity, and could be expected to serve as a drug carrier in future with broad application prospects.Keywords:chitosan nanoparticles;cyclodextrin;antioxidant activity;antitumor activity;drug release
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Ziming Yang,Puwang Li,Yu Chen,Qiang Gan,Zhipan Feng,Yiguang Jin,Chuang Zhou,Zuyu He,Chao Wang,Yunhao Liu,Changgen Feng
A novel pH/glutathione (GSH) multi-responsive chitosan nanoparticles (NPs) material has been successfully designed and prepared by a self-assembly/self-crosslinking method for photodynamic therapy (PDT), which overcomes the shortcomings of traditional photosensitizer carriers, such as poor chemical stability, low loading efficiency and single-responsive photosensitizer release. Amphiphilic sulfhydryl chitosan (SA-CS-NAC) is first prepared by modifying chitosan (CS) with stearic acid (SA) and N -acetyl-L-cysteine (NAC), and then subject to self-assembly and self-crosslinking in the presence of photosensitizer, indocyanine green (ICG), to form the ICG-loaded amphiphilic sulfhydryl chitosan nanoparticles (SA-CS-NAC@ICG NPs). The ICG entrapment efficiency and loading efficiency of the NPs are found to be 95.2% and 27.6%, respectively. The multi-responsive ICG release of the NPs to the low pH and high GSH content of the microenvironment in tumor cells is successfully achieved. Under the laser irradiation, the SA-CS-NAC@ICG NPs produce the amount of reactive oxygen species (ROS) twice of that generated by free ICG under the same conditions. The in vitro cell experiment confirmed the strong cellular uptake ability, low biotoxicity and good tumor inhibition of the NPs. Our work has provided a new strategy for the targeted photosensitizer delivery for PDT.
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ACS Applied Polymer Materials
·
2023
·
IF 5
Jingchen Gao,Xiyuran Wang,Xiangyi Kong,Xujin Yao,Jinyang Ren,Shujun Chen,Na Shen,Zhuo Li,Yitian Wang,Ye Wei,Oleg Glebov,Fengyuan Che,Qi Wan
Proline, a nonessential amino acid, has been demonstrated to possess neuroprotective capabilities in the central nervous system (CNS). However, the mechanisms through which it impacts cerebral ischemia–reperfusion (I/R) injuries remain unclear. Our study observed a decrease in proline concentrations within the ischemic brain tissues of I/R mice. Supplementing proline protected against ischemic neuronal death, both in vivo and in vitro, suggesting its potential as a neuroprotectant. However, our data indicated that proline has a low permeability to the blood–brain barrier (BBB) in mice. Therefore, it is clinically infeasible to develop proline as a stroke therapy. To develop an approach to deliver proline into the injured brain, we engineered a method to transport proline to the injured brain. We prepared chitosan nanoparticles (NPs) loaded with proline utilizing the ion cross-linking method. Our findings demonstrated that the proline-loaded chitosan NPs efficiently passed through the BBB and delivered proline into the injured brain, thereby conferring neuroprotection. Further exploration revealed that proline upregulated the level of annexin A6 (ANX6)/β1 integrin, which, in turn, increased the phosphorylation of cell-survival-promoting kinase Akt. This sequence of events consequently promotes neuronal survival following I/R. In conclusion, our study has developed a neuroprotection approach by which low-BBB-permeable proline is delivered by chitosan NPs into the brain. Furthermore, this study suggests that the neuroprotective role of proline in I/R is mediated by the ANX6/β1 integrin/Akt signaling pathway.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-14
·
IF 4.6
Alishiri Mahsa,Gonbadi Maryam,Narimani Mehdi,Abdollahi Seyyed Amirreza,Shahsavaripour Negin
The contamination of the aquatic environment with antibiotics is among the major and developing problems worldwide. The present study investigates the potential of adsorbent magnetite-chitosan nanoparticles (Fe 3 O 4 /CS NPs) for removing trimethoprim (TMP) and sulfamethoxazole (SMX). For this purpose, Fe 3 O 4 /CS NPs were synthesized by the co-precipitation method, and the adsorbent characteristics were investigated using XRD, SEM, TEM, pH zpc , FTIR, and VSM. The effect of independent variables (pH, sonication time, adsorbent amount, and analyte concentration) on removal performance was modeled and evaluated by Box–Behnken design (BBD). The SEM image of the Fe 3 O 4 /CS adsorbent showed that the adsorbent had a rough and irregular surface. The size of Fe 3 O 4 /CS crystals was about 70 nm. XRD analysis confirmed the purity and absence of impurities in the adsorbent. TEM image analysis showed that the adsorbent had a porous structure, and the particle size was in the range of nanometers. In VSM, the saturation magnetization of Fe 3 O 4 /CS adsorbent was 25 emu g −1 and the magnet could easily separate the adsorbent from the solution. The results revealed that the optimum condition was achieved at a concentration of 22 mg L −1 , a sonication time of 15 min, an adsorbent amount of 0.13 g/100 mL, and a pH of 6. Among different solvents (i.e., ethanol, acetone, nitric acid, and acetonitrile), significant desorption of TMP and SMX was achieved using ethanol. Also, results confirmed that Fe 3 O 4 /CS NPs can be used for up to six adsorption/desorption cycles. In addition, applying the Fe 3 O 4 /CS NPs on real water samples revealed that Fe 3 O 4 /CS NPs could remove TMP and SMX in the 91.23–95.95% range with RSD (n = 3) < 4. Overall, the Fe 3 O 4 /CS NPs exhibit great potential for removing TMP and SMX antibiotics from real water samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yao Wang,Liang Yang,Xiao Zhou,Ye Wang,Yijia Liang,Binshao Luo,Yuhao Dai,Zhouling Wei,Shili Li,Rong He,Wei Ding
The exploration of biopolymer-based materials to avoid hazardous chemicals in agriculture has gained enormous importance for sustainable crop protection. Due to its good biocompatibility and water solubility, carboxymethyl chitosan (CMCS) has been widely applied as a pesticide carrier biomaterial. However, the mechanism by which carboxymethyl chitosan-grafted natural product nanoparticles induce tobacco systemic resistance against bacterial wilt remains largely unknown. In this study, water-soluble CMCS-grafted daphnetin (DA) nanoparticles ( [email protected] ) were successfully synthesized, characterized, and assessed for the first time. The grafting rate of DA in CMCS was 10.05 %, and the water solubility was increased. In addition, [email protected] significantly increased the activities of CAT, PPO and SOD defense enzymes, activated the expression of PR1 and NPR1 , and suppressed the expression of JAZ3 . [email protected] could induce immune responses against R. solanacearum in tobacco, including increases in defense enzymes and overexpression of pathogenesis-related (PR) proteins. The application of [email protected] effectively suppressed the development of tobacco bacterial wilt in pot experiments, and the control efficiency was as high as 74.23 %, 67.80 %, 61.67 % at 8, 10, and 12 days after inoculation. Additionally, [email protected] has excellent biosafety. Therefore, this study highlighted the application of [email protected] in manipulating tobacco to generate defense responses against R. solanacearum , which can be attributed to systemic resistance.
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