JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
·
IF 4
Yanbo Hu,Jianing Zhou,Xianjun Zou,Ruining Zhang,Yi Cao,Yuzhu Shen,Jun Zhao
Dietary polysaccharides, widely present in plants, fungi, and algae, have been utilized as functional foods because of their excellent bioactivity. In this study, four polysaccharides (BSPS) from black soybean hulls, designated as BSPS-N-a, BSPS-N-b, BSPS-A-c, and BSPS-A-d, were efficiently extracted and purified. Their fine structures were subsequently investigated through a combination of Carbon-13 Nuclear Magnetic Resonancea ( 13 C NMR), Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS), and enzymatic hydrolysis. Monosaccharide analysis, Fourier transform infrared spectroscopy (FT-IR) analysis, and 13 C NMR analysis indicated that BSPS-N-a was a galactomannan composed of β-1,4-D-Man p and β-1,4-D-Gal p , BSPS-N-b was a galactoglucomannan composed of β-1,4-D-Gal p , β-1,4-D-Glc p and β-1,4-D-Man p ; Both BSPS-A-c and BSPS-A-d were composed of homogalacturonan (HG) with minor rhamnogalacturonan-I (RG-I) domains composed of α-1,4-D-Gal p A and β-1,4-D-Gal p , the difference was that BSPS-A-c exhibited a higher degree of branching, mainly containing a large amount of α-1,2,4-L-Rha p . To verify the results of 13 C NMR, enzymatic hydrolysis combined with MALDI-TOF-MS analysis was determined. The results showed that BSPS-N-a and BSPS-N-b could be degraded into hexose (Hex) with a degree of polymerization ranging from 2 to 9 by mannanase, and BSPS-A-c and BSPS-A-d were degraded into galacturonic acid (GalA) with a degree of polymerization ranging from 2 to 3 by endo-polygalacturonase. These results were consistent with the above results from monosaccharide analysis, FT-IR analysis, and 13 C NMR analysis. This study will provide a basis for the structure-activity relationships of black soybean hull polysaccharides (BSPS) and the development of BSPS as new functional dietary polysaccharides, natural functional foods and pharmaceuticals.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2021
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IF 7.312
Nan Hu,Keke Zhang,Yanfei Li,Tianyu Hou,Zhijun Zhang,Huizhen Li
The utilization of agro-food waste into protein products is an alternative solution for global protein shortage and waste management. Perilla ( Perilla frutescens L.) seed meal (PSM) is abundant and protein-rich by-products from the oilseed industry. To achieve the industrial production of valuable PSM protein (PSMP), a two-stage foam separation with a low cost and a high efficiency was developed to fractionate the high-concentration PSMP (1.3 times its critical micelle concentration (CMC)) from its crude extract. In the first-stage, glycine betaine (GB), a naturally occurring osmolyte, was used as a green enhancer for the recovery of PSMP. The result of SEM, FTIR, UV–vis and fluorescence spectra demonstrated that GB engaged in cation-π interactions with aromatic residues of PSMP, which made the protein structure unfold and enabled its hydrophobic groups of PSMP to be more exposed. These factors, plus protein aggregation, were responsible for the enhanced interfacial adsorption of PSMP. Its recovery percentage with 0.3 g/L GB (pH 7.0) increased by 52.9% compared to that without GB. Furthermore, an internal component of regular-hexagon hollow prismoid (RHP) was also designed to intensify the foam drainage and raise PSMP enrichment to 7.1 ± 0.3. In the second-stage, PSMP could be further recovered and its total recovery percentage reached 94.5 ± 4.7%. After foam separation, the amino acid composition of PSMP was analyzed by SDS-PAGE and HPLC–UV and its functional properties was also evaluated. These results provide a new insight into enhancing the protein separation from agro-food waste.
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BIOCATALYSIS AND BIOTRANSFORMATION
·
2020
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IF 1.863
Chou Li,Qingzhi Ji,Tongyao He,Yingying Liu,Yunqing Ma
Deconjugation of bile salts in vivo not only promotes the cholesterol metabolism of the host, but also provides cellular carbon, nitrogen and sulphur for some probiotics. In this study, the gene of BSH from Bifidobacterium bifidum (ATCC 29521) was amplified and expressed using glutathione S-transferase (GST) Gene Fusion System. The enzyme was purified in a mild conditions without denaturation and renaturation, and the molecular mass was estimated to be 35 kDa. The optimum pH for the enzyme catalysis was 5.5. The BSH enzyme was stable from 37 to 57 °C. In addition, this enzyme was completely inhibited by Cu2+, while many other metal ions only gave moderate inhibition. Hydrolysis efficiency of BSH was quantified in two ways: ninhydrin colouration method and high-performance liquid chromatography coupled with evaporative light scattering detector (HPLC-ELSD). The hydrolysis process was clearly observed through real-time picture monitoring. The activity of BSH on glycine-conjugated bile salts was better than on taurine-conjugated bile salts. The proportion of hydrolysis for both sodium glycochenodeoxycholate (GCDCA) and sodium glycoursodeoxycholate (GUDCA) were more than 90% in 60 min. However, the proportion of hydrolysis for sodium taurochenodeoxycholate (TCDCA) and sodium tauroursodeoxycholate (TUDCA) were only 68% and 43% at the same condition. The study of this paper provides a better choice for hydrolysis of glycine-conjugated bile salts.HighlightsThe specificity of BSH was high for glycine-conjugated bile salts.Hydrolysis process of bile acid was obtained through real-time picture monitoring.Hydrolysis efficiency of BSH was quantified in two ways: ninhydrin colouration method and HPLC-ELSD.
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ACTA PHARMACOLOGICA SINICA
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2023
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IF 8.2
Wu Jun,Zhao Miao,Jin Yu-chen,Li Min,Yu Ke-xin,Yu Hai-bo
Epilepsy is a prevalent and severe neurological disorder and approximately 30% of patients are resistant to existing medications. It is of utmost importance to develop alternative therapies to treat epilepsy. Schisandrin B (SchB) is a major bioactive constituent of Schisandra chinensis (Turcz.) Baill and has multiple neuroprotective effects, sedative and hypnotic activities. In this study, we investigated the antiseizure effect of SchB in various mouse models of seizure and explored the underlying mechanisms. Pentylenetetrazole (PTZ), strychnine (STR), and pilocarpine-induced mouse seizure models were established. We showed that injection of SchB (10, 30, 60 mg/kg, i.p.) dose-dependently delayed the onset of generalized tonic-clonic seizures (GTCS), reduced the incidence of GTCS and mortality in PTZ and STR models. Meanwhile, injection of SchB (30 mg/kg, i.p.) exhibited therapeutic potential in pilocarpine-induced status epilepticus model, which was considered as a drug-resistant model. In whole-cell recording from CHO/HEK-239 cells stably expressing recombinant human GABA A receptors (GABA A Rs) and glycine receptors (GlyRs) and cultured hippocampal neurons, co-application of SchB dose-dependently enhanced GABA or glycine-induced current with EC 50 values at around 5 μM, and application of SchB (10 μM) alone did not activate the channels in the absence of GABA or glycine. Furthermore, SchB (10 μM) eliminated both PTZ-induced inhibition on GABA-induced current ( I GABA ) and strychnine (STR)-induced inhibition on glycine-induced current ( I glycine ). Moreover, SchB (10 μM) efficiently rescued the impaired GABA A Rs associated with genetic epilepsies. In addition, the homologous mutants in both GlyRs-α1(S267Q) and GABA A Rs-α1(S297Q)β2(N289S)γ2L receptors by site-directed mutagenesis tests abolished SchB-induced potentiation of I GABA and I glycine . In conclusion, we have identified SchB as a natural positive allosteric modulator of GABA A Rs and GlyRs, supporting its potential as alternative therapies for epilepsy.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2023
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IF 2.6
Xia Li,Weiping Liu,Yahui Yin,Yu Zhou,Hongfei Xiang,Xianzhen Xu
Vapor–liquid equilibrium (VLE) is essential for separation processes, such as distillation and absorption in chemical production. In this work, the VLE of glycine, l-histidine, and l-tryptophan aqueous solutions was measured at pressures ranging from 5.3 to 101.1 kPa. Xu’s model was used, and the model was based on the nonrandom two-liquid (NRTL) model, which has been successfully correlated with VLE data at temperatures between 298.15 and 373.85 K, and the model parameters were obtained. In addition, the absorption peaks of three amino acid aqueous solutions were analyzed by Fourier transform infrared (FTIR) spectroscopy from a microscopic perspective. The results showed that the higher the solubility of amino acids in water, the stronger the hydrogen-bonding interaction with water. Furthermore, the total mean interaction energy of water in the systems was studied by using a COSMOthermX 2021 tool. As inferred from the calculations, the total mean interaction energy was slightly affected by the concentration. The structure–activity relationship was established between the microscopic interactions and the macroscopic VLE.
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JOURNAL OF DISPERSION SCIENCE AND TECHNOLOGY
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2023
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IF 2.2
Wenxi Zhu,Xiuhua Zheng
The development of high-temperature and salt-resistant foaming agents is critical to the successful drilling of complex and low-pressure reservoirs. In this study, a novel alkyl glycine surfactant (AGS-8) was synthesized by the one-step method. The foam quality of AGS-8 at high temperatures (140–180 °C) and the salt solutions (5–36%wt) was evaluated by the Waring-Blender method to explore its applicability in harsh reservoirs. Results show that: (1) The successful synthesis and chemical structure of AGS-8 was determined by FTIR and 1H-NMR. (2) AGS-8 shows good thermal stability within 330 °C and has high surface activity. (3) AGS-8 generated stable foams after aging at 180 °C for 16 h. The outstanding high-temperature foam stability of AGS-8 is an important feature different from the conventional commercial foaming agents. (4) AGS-8 generated stable foams in saturated salt solution (36%wt NaCl) after 150 °C aged. A synergistic foam stabilization effect was found between high concentrations of NaCl (≥25%wt) and AGS-8. (5) The foam stabilization mechanism of AGS-8 was discussed and can be summarized as the electrostatic attraction effect of head groups and the enhancement of inter-molecular repulsive force. In conclusion, AGS-8 was found to be a promising foaming agent in the drilling of high-temperature salt-gypsum formations.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2019
·Vol. 411
·期 27
·7105-7113
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IF 3.286
Zhang Nana,Li Jian,Zhang Panpan,Yang Xiaodi,Sun Chong
A novel electrochemical approach for determination of arachidonic acid (ARA) was developed based on the linear arginine-glycine-aspartic-Au (RGD-Au) nanomaterial modified on glassy carbon electrode (GCE). The prepared material was characterized by transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The electrochemical signal was obtained from the reduction of 1,4-naphthoquinone and ARA served as a proton source. Under the optimum experimental conditions, the RGD-Au-based electrode was used to analyze ARA. Meanwhile, the electrochemical characteristics were also studied by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and linear sweep voltammetry (LSV). The sensor showed a wider linear range from 0.5 to 100 μM and the linear fitting equation was I p (μA) = 0.0721 c + 2.4583 ( R 2 = 0.9987) with a detection limit of 80 nM. The application of the sensor in real samples was tested and compared with that of LC-MS/MS. This sensor would be a promising platform for detection of ARA in blood plasma.
Graphical abstract
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JOURNAL OF CHEMICAL THERMODYNAMICS
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2016
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IF 2.196
Hujun Xie,Lijiang Zhao,Chengcheng Liu,Yifan Cao,Xiaoxing Lu,Qunfang Lei,Wenjun Fang
The densities of aqueous solutions of glycine, l -serine, l -alanine, l -proline with the ionic liquid (IL), 1-phenylpiperazinium tetrafluoroborate ([Phpi][BF 4 ]) at the IL concentrations of (0.025, 0.055 and 0.100) mol·kg −1 have been measured at the temperatures of (298.15, 303.15 and 308.15) K. On the basis of the experimental results, the apparent molar volume ( V Ф ), standard partial molar volume ( V Ф 0 ), transfer partial molar volume (Δ tr V Φ 0 ) and hydration number ( n H ) have been calculated. The hydrophilic–hydrophilic, hydrophobic–hydrophilic and hydrophobic–hydrophobic interactions are involved in the studied systems of {[Phpi][BF 4 ] + amino acids + H 2 O. These volumetric parameters can help to understand the mixing effects and other complex biological processes between amino acids and ionic liquid aqueous solution.
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ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Yongjing Li,Jiaxun Wan,Zihao Zhang,Jia Guo,Changchun Wang
The development of multifunctional ultrasound contrast agents has inspired considerable interest in the application of biomedical imaging and anticancer therapeutics. However, combining multiple components that can preferentially accumulate in tumors in a nanometer scale poses one of the major challenges in targeting drug delivery for theranostic application. Herein, reflux-precipitation polymerization, and N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide-meditated amidation reaction were introduced to effectively generate a new type of soft glycine/poly(ethylene glycol) (PEG)/RGD-modified poly(methacrylic acid) nanobubbles with a uniform morphology and desired particle size (less than 100 nm). Because of the enhanced biocompatibility resulting from the glycine modification, over 80% of the cells survived, even though the dosage of glycine-modified polymeric nanobubbles was up to 5 mg/mL. By loading doxorubicin as an anticancer drug and perfluorohexane as an ultrasound probe, the resulting glycine/PEG/RGD-modified nanobubbles showed remarkable cancer therapeutic efficacy and a high quality of ultrasonic imaging; thus, the ultrasonic signal exhibited a 1.47-fold enhancement at the tumor site after intravenous injection. By integrating diagnostic and therapeutic functions into a single nanobubble, the new type of theranostic nanobubbles offers a promising strategy to monitor the therapeutic effects, giving important insights into the ultrasound-traced and enhanced targeting drug delivery in biomedical applications.
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Desalination and Water Treatment
·
2025
·
IF 1
Gaole Yan,Hongtao Wu,Haining Ja,Xianghai Li,Gang Yang
Developing highly selective phosphate adsorbents with abundant binding sites presents a significant challenge. In this study, we synthesized an amine-functionalized hydrotalcite (TEA-Gly-LDH) with a high specific surface area using triethanolamine and glycine, which was employed as an adsorbent for capture phosphate from water. We report the phosphate adsorption properties, isothermal behavior, and adsorption kinetics. The results indicate that TEA-Gly-LDH exhibits a higher adsorption capacity and a faster adsorption rate, achieving equilibrium within 2 hours at 25 °C. The removal rate reached as high as 96 %, with an adsorption capacity of 59.43 mg/g, which is 11 % greater than that of unmodified hydrotalcite. Furthermore, it maintains a high phosphate adsorption capacity across a wide pH range of 3–11. Additionally, TEA-Gly-LDH demonstrates excellent selectivity for phosphate, with a distribution factor of 2.73 × 10 4 in a mixed-ion system, surpassing the 2.39 × 10 3 of unmodified hydrotalcite. The adsorption capacity is preserved even after five regeneration cycles. The adsorption data fit well to the Langmuir isotherm model, suggesting that phosphate adsorption is a monolayer chemisorption process. Kinetic studies reveal that the material's adsorption behavior is primarily governed by ion exchange, adsorption at surface active sites, and intraparticle diffusion.
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RSC Advances
·
2025
·Vol. 15
·期 5
·3331-3338
·
IF 4.6
Dinghua Yu,Jianqiang Wang,Guowei Wang
Silane coupling agents play an indispensable role in improving interfacial adhesion of composite materials, but their interaction mechanism is often unclear. This article combines experiments and theoretical calculations to reveal the importance of hydrogen bonds between silane coupling agents and the matrix polyamide 6 in improving the mechanical properties of composite materials. Firstly, glycine bridged silane (GBSilane) was synthesized and the structure was confirmed by FT-IR, 1H NMR and HRMS. Secondly, with glass fiber treated using GBSilane as a filler, the mechanical properties of glass fiber/PA6 composite materials were studied. Compared with untreated glass fiber/PA6 composites, under the optimal treatment concentration of 1.5%, the tensile strength of glass fiber/PA6 composites treated with 3-aminopropyl triethoxysilane (APTES) and GBSilane increased by 41% and 67%, respectively, and the notch impact strength increased by 55% and 96.5%, respectively. Lastly, density functional theory (DFT) calculations revealed that stronger hydrogen bonds have formed between GBSilane and PA6 than APTES, which have induced the stronger PA6–GBSilane binding energy of 58.20 kJ mol−1. By comparison, the binding energy of PA6–APTES is only 30.91 kJ mol−1. These results demonstrated that the as-synthesized GBSilane could improve the mechanical properties of PA6 composites through an enhanced hydrogen bonding mechanism.
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ENERGY
·
2024
·
IF 9
Yali Liu,Ming Li,Reda Hassanien Emam Hassanien,Yunfeng Wang,Runsheng Tang,Ying Zhang
A shape-stable glycine water-based phase-change material (GPCM) was fabricated herein via natural adsorption using modified expanded graphite (MEG) as a porous carrier. Cetyltrimethylammonium bromide (CTAB) was introduced as a modifier on the surface of expanded graphite (EG) to synthesise MEG, which improved compatibility between EG and GPCM. Furthermore, the adsorption capacities of EG and MEG on GPCM were compared and analysed. The thermophysical properties of the as-prepared MEG/GPCM composite were evaluated using differential scanning calorimetry. Results revealed that MEG modified with 0.1 g of CTAB (MEG-0.1) had the best compatibility with GPCM and its adsorption capacity increased from 73.96% to 84.05% compared with that of unmodified EG. When the MEG content was 14%, the MEG/GPCM composite had a suitable melting temperature of −5.06 °C and high latent heat of 243.80 J/g. Moreover, the thermal conductivity was 1.836 W/(m⋅K), 216.33% higher than that of GPCM. After 100 thermal cycles, and the melting temperature and latent heat of the MEG/GPCM composite only decreased by 1.38% and 6.15% to −5.13 °C and 228.79 J/g, respectively. Thus, the prepared composites exhibited excellent thermal properties and showed great potential in the application of low-temperature cold energy storage and cold-chain transportation.
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