ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Yang Wang,Yingying Wei,Pengcheng Gao,Si Sun,Qian Du,Zhifei Wang,Yong Jiang
Magnetic material is considered to as a major concern material for the enrichment of histidine-rich proteins (His-proteins) via metal-ion affinity. In this work, magnetic polymer microspheres with core–shell structure (Fe3O4@PMAA@Ni) were successfully prepared via reflux-precipitation polymerization followed by in situ reduction and growth of Ni2+. The obtained Ni nanofoams with flower-like structure and uniform pore size (3.34 nm) provided numerous binding sites for His-proteins. The adsorption performance of Fe3O4@PMAA@Ni microspheres for His-proteins was estimated via selectively separating bovine hemoglobin (BHb) and bovine serum albumin (BSA) from a matrix composed of BHb, BSA, and lysozyme (LYZ). The results indicated that Fe3O4@PMAA@Ni microspheres could efficiently and selectively separate His-proteins from the matrix, with a maximum adsorption capacity of ∼2660 mg/g for BHb. Moreover, Fe3O4@PMAA@Ni microspheres exhibited good stability and recyclability for BHb separation over seven cycles. Therefore, this work reported a novel and facile strategy to prepare core–shell Fe3O4@PMAA@Ni microspheres, which was promising for practical applications of His-protein separation and purification in proteomics.
研究领域:
生物化学
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蛋白质组学
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材料科学
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DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yixun Liang,Jiawei Liu,Lili Wang,Yi Wan,Jiwei Shen,Quan Bai
A metal affinity-carboxymethyl cellulose functionalized magnetic graphene, namely MGCI-Cu composite, was prepared by successive modifications of graphene oxide nanosheets with magnetic nanoparticles , carboxymethyl cellulose (CMC), iminodiacetic acid (IDA) and then chelated with copper ions . The successful modifications of the graphene surface were demonstrated by various characterizations, and a high density of 6.17 μmol m −2 for metal affinity groups was obtained. The composite exhibited high adsorption selectivity toward histidine-rich proteins. The adsorption was governed by strong metal affinity binding force between hisitidine residues of proteins and immobilized Cu 2+ ions of MGCI-Cu composite. In particular, highly selective isolation of hemoglobin (Hb) was achieved in 0.2 mol L −1 phosphate buffer at pH 8. The adsorption capacity of Hb significantly increased to 769 mg g −1 in comparison to that of 435 mg g −1 on metal affinity modified magnetic graphene composite (MGI-Cu) without CMC modification. The adsorbed Hb molecules were recovered with a carbonate buffer (0.2 mol L −1 pH 10) containing 0.5 mol L −1 imidazole . MGCI-Cu composite displayed favorable reusability for at least four times after regeneration of the composite by edetic acid (EDTA) and Cu 2+ solution. The practical applications demonstrated that MGCI-Cu composite could highly selectively isolate Hb from human whole blood and polyhistidine-tagged recombinant protein from Escherichia coli ( E. coli ) lysate.
研究领域:
材料科学
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生物技术
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分析化学
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蛋白质工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Shaobo Wang,Shuncheng Yao,Quanhong Hu,Zhuo Wang,Yunchao Zhao,Linlin Li
Photothermal therapy (PTT) relying on photothermal conversion agents (PCAs) to generate local hyperthermia and eliminate tumors is considered as a high-efficiency localized treatment method for cancer. However, PTT inadvertently exacerbates tumor immune evasion and recurrence, and leads to tumor metastasis through producing excessive free radicals and inflammatory responses at the tumor microenvironment (TME). Facing this challenge, here, we designed metal-quercetin-histidine co-coordinated nanoparticles (FQH NPs) as a multifunctional PCA with capacity of reversing immunosuppressive TME and activating the “cold” tumor to “hot”. Through co-coordination guided band-ligand-to-metal charge transfer (LMCT), the self-assembled FQH NPs exhibit robust photothermal conversion capabilities, efficiently scavenge various ROS generated during PTT, and decrease excretion of pro-inflammatory factor interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Furthermore, FQH NPs remarkably decrease the expression of programmed death ligand 1 (PD-L1) on cancer cells, thereby increasing activation of T cells in tumors. Combining with the immunogenic cell death (ICD) effect during PTT, this immune-checkpoint inhibition effect conquers the “cold” orthotopic breast cancer, prevents liver/lung metastasis, and also inhibits the growth of distal tumors through immune memory effect. It offers perspectives and potential avenues for improving photothermal cancer therapy.
研究领域:
肿瘤学
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纳米医学
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免疫学
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DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 15
·6817-6825
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IF 3.591
Jiawei Liu,Yingying Liu,Yixun Liang,Fen Ma,Quan Bai
In this work, we prepared poly-L-lysine (PLL) functionalized magnetic graphene through a simple covalent bonding process. The composite was further conjugated with aspartic acid (Asp) and chelated copper ions to obtain a metal affinity PLL-functionalized magnetic graphene composite (MGPLA-Cu). Various characterizations via FT-IR, XRD, TGA, VSM and SEM/TEM confirm its physicochemical properties. Owing to the strong metal affinity forces between metal affinity groups and the residual histidine in proteins, the MGPLA-Cu composite exhibits highly selective adsorption performance toward histidine-rich proteins, i.e., hemoglobin (Hb) in this case. A high adsorption efficiency of ca. 100% was achieved for 200 mg L−1 Hb in a phosphate buffer at pH 8. The equilibrium adsorption isotherm of Hb on the MGPLA-Cu composite well fitted to the Langmuir theoretical model (R2 = 0.9969), along with the maximum adsorption capacity (qmax) at 334 mg g−1. The adsorption kinetics was favorably described by the pseudo-second-order kinetic model. The retained Hb could be effectively recovered with a 0.2 mol L−1 carbonate buffer containing 0.05 mol L−1 imidazole at pH 10, giving rise to a recovery of ca. 97%. The MGPLA-Cu composite showed favorable reusability, and a high adsorption efficiency (>80%) was maintained after five adsorption–desorption operation cycles. The practical application demonstrates that the MGPLA-Cu composite can isolate Hb from human whole blood with high selectivity and high purity.
研究领域:
材料科学
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生物化学
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蛋白质工程
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Changqing Guo,Guanghui Chen,Ning Wang,Shougui Wang,Yuwen Gao,Jipeng Dong,Qing Lu,Fei Gao
Several novel multifunctional histidine-based hypercrosslinked ionic polymers (HIPs) were constructed via a one-pot copolymerization strategy. The resultant HIPs were characterized by various technologies and investigated for the CO 2 adsorption and conversion. Plentiful hydrogen bond donors, nucleophilic ionic sites, and Lewis bases were successfully integrated into the HIPs skeletons. As-prepared histidine-based HIPs possess excellent hierarchical pore structure. The optimal HIP-Br-His exhibits a high CO 2 capture capacity of 2.90 mmol/g at 273 K and 1 bar, much higher than that of corresponding hypercrosslinked polymer HCP-Br without histidine units. Meanwhile, the HIP-Br-His achieves an excellent catalytic activity of 95 % product yield with 99 % product selectivity in CO 2 cycloaddition to propylene oxide under metal-, cocatalyst- and solvent-free and mild conditions (70 °C and 1.0 MPa). Particularly, HIP-Br-His can efficiently capture and convert dilute CO 2 into cyclic carbonates with a satisfactory catalytic performance. The excellent catalytic activity associating with the confirmed good stability, recyclability, and substrate compatibility make the developed histidine-based HIPs a promising heterogeneous catalyst for efficient CO 2 capture and conversion.
研究领域:
材料科学
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环境科学
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化学工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Shen Zhang,Meiqi Bai,Jing Qian,Yuyu Guo
In terms of the vital functions of barbaloin, it is necessary to develop a selective and sensitive detection means in barbaloin sensing to replace the previous defective analytical methods. Herein, the water-soluble luminescent gold nanoclusters (Au NCs) with good emission character were firstly established for barbaloin detection with high efficiency. The small size and stable probe was synthesized through one-pot way by protecting and reducing of histidine (His). The characterization results revealed that the quenching mechanism was mainly attributed to the inner filter effect (IFE). The His-Au NCs displayed high selectivity to barbaloin against interferes and a good linear relationship existed in the range of 0.5–150 μM. More importantly, the probe was successfully employed to detect barbaloin in bovine serum samples with satisfactory recoveries and the excellent performance was also obtained from temperature sensing. This novel detection platform expanded the analytical methods for barbaloin based on fluorescence properties.
研究领域:
分析化学
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纳米生物技术
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药物分析
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DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 66
·53835-53845
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IF 3.84
Weihua Jiang,Lei Yang,Lipeng Qiu,Jingwen Xu,Xiuchun Yang,Ju Wang,Hui Zhou,Dongkai Wang
Multifunctional hybrid nanoparticles (NPs) based on sodium carboxymethylcellulose-graft-histidine (CMH) and TPGS were designed for effective delivery and overcoming multidrug resistance (MDR) of docetaxel (DTX). An amphiphilic CMH copolymer with excellent biocompatibility, pH-sensitivity, and solubilizing capacity for poorly soluble drugs was firstly synthesized and characterized. TPGS incorporated into CMH copolymer facilitated lower critical aggregation concentration (CAC) (0.0074 mg mL−1), smaller particle size (147.8 ± 3.14 nm), higher drug encapsulation (88.3 ± 3.33%), and better controlled release rate. The NPs based on CMH copolymer showed a pH-dependent drug release manner. The DTX-loaded CHT-1.5 NPs could significantly enhance cytotoxicity against both MCF-7 and MCF-7/ADR cells when compared to DTX-loaded CMH NPs and free DTX. Furthermore, cellular uptake and P-gp inhibition assays confirmed that the enhanced MDR reversal effect of DTX-loaded CHT-1.5 NPs was caused by the combination of increased drug accumulation and inhibition of P-glycoprotein mediated drug efflux in drug-resistant MCF-7/ADR cells. Consequently, CHT-1.5 NPs can act as an effective nanocarrier for delivering antitumor drugs and overcoming P-gp overexpressing tumor cells.
研究领域:
药学
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纳米技术
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癌症研究
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药物递送系统
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DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 15
·6133-6138
·
IF 1.821
Li-Qiang Yan,Yun Ma,Mei-Fang Cui,Zheng-Jian Qi
A coumarin-based “Turn-on” fluorescence chemosensor 1 containing L-histidine for Al3+ was readily synthesized and investigated, which showed high selectivity and remarkable sensitivity to aluminium(III) ions based on a photo-induced electron-transfer (PET) mechanism in aqueous solution (Tris–HCl, 0.1 mM, pH 7.2). The sensor responded rapidly to Al3+ in aqueous solutions with a 1 : 1 stoichiometry. Meanwhile, it showed excellent adaptability and also responsiveness.
研究领域:
分析化学
·
生物化学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2014
·Vol. 2
·期 37
·6207-6214
·
IF 0
Jiangnan Zheng,Zian Lin,Wei Liu,Ling Wang,Sen Zhao,Huanghao Yang,Lan Zhang
This work reports a facile ligand-free method for the rapid and highly specific separation of histidine (His)-rich proteins using CuFe2O4 magnetic nanocrystal clusters (MNCs). Monodispersed CuFe2O4 MNCs were synthesized via a simple and economical one-pot hydrothermal process. The resulting MNCs were characterized in detail. The measurements indicated that the MNCs exhibited good dispersion, high crystallinity, and superparamagnetic properties. Moreover, the obtained MNCs had a high saturation magnetization (45.1 emu g−1), which was sufficient to accomplish fast and efficient separation with an external magnetic field. The selectivity and binding capacity of CuFe2O4 MNCs were evaluated using a His-rich protein (bovine haemoglobin) and other proteins (bovine serum albumin, human serum albumin, myoglobin, lysozyme, cytochrome c and horseradish peroxidase) containing fewer surface-exposed His residues as model samples. The most distinct feature of the CuFe2O4 MNCs is the high haemoglobin binding capacity (4475 mg g−1) due to the coordination between copper(II) ions and surface-exposed histidine resides of haemoglobin. In addition, the CuFe2O4 MNCs can be successfully employed to selectively bind and remove abundant haemoglobin from human blood samples. The good results demonstrate the potential of CuFe2O4 MNCs in the separation of His-rich proteins.
研究领域:
生物化学
·
材料科学
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医学
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产品关联
DOI
PubMed
RSC Advances
·
2015
·Vol. 5
·期 19
·14138-14146
·
IF 3.84
Jiashan Zhou,Yan Li,Haiqing Dong,Hua Yuan,Tianbin Ren,Yongyong Li
Polymeric catiomers, which can mimic viruses for gene packing and delivery, have received considerable attention as nonviral vectors for gene therapy. Inspired by the critically important role of the sequence of structural units in various biomolecules, including DNA and protein, in this study, we intend to investigate how the monomer sequence of a polymeric catiomer affects its gene packing capacity and delivery efficiency. The well-documented poly(histidine-co-lysine) was chosen as the scaffold for gene carrier. Four reducible polycations (RPCs) based on sequence-regulated peptides monomers were synthesized. Chemical parameters (namely, composition and molecular weight) of four RPCs were controlled at comparable level except for the monomer sequence. All of the RPCs exhibited low cytotoxicity and effective DNA binding ability. However, these RPCs displayed distinct diversity from each other, especially in their ability of binding to DNA, buffering capacity and transfection efficiency. Using 293T cell as the mode, we found that the regulation of the monomer sequence of polycations could significantly affect their properties for gene delivery, with differences of 100 fold. The sequence effect might be correlated with different chain folding as well as physiochemical properties of RPCs/pDNA complexes, providing new insight for designing gene vector with promising prospects in gene therapy.
研究领域:
生物医学工程
·
分子生物学
·
基因治疗
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产品关联
DOI
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 5
·2947-2958
·
IF 4.036
Jing Wang,Chengbao Liu,Bei Qian
A novel L-histidine based ionic liquid (LHIL) was developed and successfully synthesized. Its structure was confirmed by Fourier-transform infrared spectroscopy, UV-vis spectroscopy, X-ray photoelectron spectroscopy, 1H-NMR and high-resolution mass spectrometry. The outstanding corrosion inhibition effect of the LHIL on mild steel in 1 M hydrochloric acid was thoroughly evaluated by Tafel plots, electrochemical impedance spectroscopy, and localized electrochemical strategies. The results revealed that the corrosion of mild steel was effectively suppressed by the adsorption of LHIL on its surface, and the best inhibition efficiency reached 98.8%. The adsorption behavior of LHIL on steel obeyed the Langmuir adsorption isotherm, which involved both chemisorption and physisorption. Theoretical calculations indicated the strong chemisorption of LHIL on steel, as proved by the low energy gap (ΔE = 0.0522 eV) and high binding energy (Ebinding = 303.47 kcal mol−1), which clearly confirmed the effectiveness of LHIL for steel protection.
研究领域:
材料科学
·
腐蚀科学
·
电化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Sisi Tang,Xiaodan Wu,Pengfei Zhao,Kangling Tang,Yu Chen,Jinli Fu,Shu Zhou,Zhaoxia Yang,Zhaohui Zhang
This work firstly developed a near-infrared fluorescence capillary imprinted sensor with high selectivity and sensitivity for the chiral recognition of l -histidine. The near-infrared fluorescence imprinted polymer prepared by sol-gel method using CdTe quantum dots as the near-infrared fluorescence source was self-sucked into activated capillary to form the fluorescence imprinted capillary. The fluorescence imprinted capillary sensor had higher fluorescence response efficiency. With the superior stability, reproducibility and reusability, the fluorescence capillary imprinted sensor displayed higher selectivity toward l -histidine. Under the optimal conditions, the fluorescence intensity (λex = 370 nm, λem = 695 nm) of the fluorescence capillary imprinted sensor was enhanced in proportion to l -histidine concentration ranged from 0.1 pM to 1.8 pM with a limit of detection of 0.08 pM. Spiking experiment showed that the fluorescence capillary imprinted sensor was successfully used for determination of l -histidine in human urine and serum with the recoveries of 99.6–102.7%. The near-infrared fluorescence capillary imprinted sensor possessed significantly potential for high sensitive detection of l -histidine, which realized trace-level analysis for micro-volume sample and provided promising utility for green chemical.
研究领域:
分析化学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）