FASEB JOURNAL
·
2025
·Vol. 39
·期 7
·e70529
·
IF 4.2
Hai-Xiang Guo,Zhong-Hao Ji,Bing-Bing Wang,Yu Xiao,Jin-Ping Hu,Yi Zheng,Wei Gao,Bao Yuan
Ulcerative colitis (UC) is a recurrent, chronic disease whose main symptoms include weight loss, diarrhea, and blood in the stool. In recent years, the incidence of UC has been increasing year by year, which seriously affects the daily life of patients. Luteolin (Lut), as a flavonoid, is widely found in a variety of vegetables and fruits and has been shown to have a variety of pharmacological activities. This work investigated the effects of Lut on dextrose sodium sulfate (DSS)-induced ulcerative colitis (UC) in mice, with a special focus on the role of endoplasmic reticulum (ER) stress in this. The outcomes demonstrated that colitis symptoms, including disease phenotype, elevated inflammatory factor levels, intestinal barrier damage, and gut microbiota disruption, were considerably alleviated in UC model mice treated with luteolin. Also, Lut alleviated ER stress and apoptosis in UC mice. We then explored the effects of Lut on ER stress and apoptosis induced by thapsigargin (TG) and tunicamycin (TM) in HT29 cells in vitro. It was found that Lut treatment inhibited TM/TG-induced ER stress and apoptosis. However, these inhibitory effects of Lut were attenuated by SIRT1 silencing in TM-treated HT29 cells. In conclusion, our results suggest that Lut supplementation in a mouse model of colitis improves the symptoms of colitis in mice, which provides a theoretical basis for further application of Lut in the prevention of inflammation-related diseases in humans. Graphical Luteolin modulates endoplasmic reticulum stress by targeting SIRT1 to ameliorate ulcerative colitis in mice.
研究领域:
药理学
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炎症性疾病
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分子生物学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xiangrong Li,Wanqing Xu,Xianfei Liu,Zhizhi Song,Zheng Li,Zihan Jia
In this study, the interaction mechanism of papain and bromelain with apigenin and luteolin in binary and ternary systems was compared and analyzed by spectroscopic methods, molecular docking, and molecular dynamics simulation. Additionally, the effects of apigenin and/or luteolin on the conformational changes and enzymatic activity of papain and bromelain were investigated. The results show that apigenin and/or luteolin exhibit a moderate binding affinity with papain/bromelain, with the binding constants K a in the range of 10 4 –10 5 L mol −1 . The order of addition of apigenin and luteolin significantly influenced their binding affinities to the enzymes. The main non-covalent forces of the papain-luteolin (first)-apigenin ternary system are hydrogen bonds and Van der Waals forces. Hydrophobic interactions, electrostatic forces and hydrogen bonds are the main non-covalent forces of other binary and ternary systems. These systems differentially alter the microenvironment of Trp and Tyr residues, secondary and tertiary structures, and enzymatic activity. Molecular docking and dynamics simulations identified specific binding sites of apigenin and/or luteolin on papain/bromelain, corroborating experimental findings. Notably, the ternary system exhibits a stronger inhibitory effect on papain activity compared to binary systems.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
Materials Today Bio
·
2026
·
IF 10.2
Xiaowen Zheng,Wenqi Wang,Xiaolong Wei,Jianguo Niu,Wei Zhang,Zhenxun Lin,Wei Wei,Shipeng Ning,Min Wang,Xianwen Wang,Qingjun Wei
Precise spatiotemporal control of inflammation serves as an effective means to regulate the inflammatory microenvironment and promote wound healing. This study constructed strontium‒luteolin (Sr‒Lut) nanoparticles with a spherical morphology, which demonstrated outstanding radical scavenging capacity through synergistic metal‒ligand effects. Specifically, Sr-Lut efficiently scavenges reactive oxygen species, inhibits the TNF-α/NF-κB and JAK-STAT signaling pathways, promotes M2 polarization, and simultaneously reduces the proinflammatory factor IL-1β and increases the anti-inflammatory factor IL-10. Transcriptomic analysis revealed that Sr-Lut reprogrammed macrophages to downregulate Ccl4 and Retnlg while upregulating extracellular matrix remodeling-associated genes. In vivo experiments demonstrated that Sr-Lut accelerated wound closure in a dose-dependent manner, achieving a healing rate of 94.33% by day 11, which was significantly greater than the 58.54% reported in the control group, while also enhancing re-epithelialization and collagen deposition. Wound tissue RNA sequencing revealed that Sr-Lut inhibits the IL-17, Toll-like receptor and NF-κB signaling pathways while promoting the expression of genes associated with epidermal structural components. In summary, a dual-function nanocomposite with both anti-inflammatory and tissue-repair capabilities has been developed, offering a promising immunomodulatory strategy for wound treatment.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Xue Bai,Shucheng Liu,Jinxin Liu,Yue Ma,Wenli Zhang,Jianming Pan
Boronate affinity-based single-hole hollow imprinted polymers (BA-H-MIPs) were fabricated and sealed in dialysis bags for highly specific adsorption of cis -diol containing luteolin (LTL). This preparation strategy is implemented through the direct imprinted polymerization reaction occurring on the surface of carboxyl-capping polystyrene colloids (CPS), and the creating mechanism of single hole is the concomitant microphase separation and symmetrical volume shrinkage of imprinted shells. BA-H-MIPs possess uniform size (200 nm), single holes (50 nm) and hollow structure, and high-affinity sites density (0.27 mg m −2 ). In static adsorption, BA-H-MIPs show specific high-capacity uptake of target LTL at 318 K (46.96 mg g −1 ), fast capture kinetics (200 min), remarkable selectivity and regeneration performances. By a pH-responsive purification process, a commercially available 85% LTL could be simply concentrated to 95.88% by BA-H-MIPs, and the purified products have the similar antibacterial performance with standard substance. In addition, the special structure of hollow interior surfaces and single holes provide high site accessibility and abundant imprinted sites, which is the key factor for improving selective binding performance.
研究领域:
材料科学
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药物化学
·
生物技术
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Qiang Jia,Yue Ma,Yinxian Peng,Yanhong Liu,Wenli Zhang
Molecular imprinted hollow SnO 2 microspheres (Ho-SnO 2 @MIPs) integrated with boronate affinity ability were fabricated via atom transfer radical polymerization, and then they were adopted as ideal adsorbents for cis -diol containing luteolin (LTL) recognition and separation. Ho-SnO 2 @MIPs possessed uniform size (200 nm), porous and hollow structure, appropriate BET surface area (118.6 m 2 g −1 ), and high-density boronate affinity sites (0.22 mg m −2 ), endowing them highly specific uptake and fast separation properties. Due to the merit of pH responsive boronate affinity, the reversible interaction of boronic acid groups and LTL was regulated by the pH value facilely, which was benefit for the controlled recognition and release. In the batch mode experiments, the quick binding equilibrium (within 60 min) and a rather high specific binding capacity (46.81 mg g −1 ) at 298 K were observed, and Ho-SnO 2 @MIPs also displayed high rebinding selectivity to LTL. By a purification process, a commercially available LTL with 85% purity could be easily extracted and concentrated to 95.28% purity by Ho-SnO 2 @MIPs, and the purified products possessed the similar antibacterial performance with standard substance.
研究领域:
材料科学
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生物化学
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产品关联
DOI
高影响因子
Micro & Nano Letters
·
2020
·Vol. 15
·期 7
·425-429
·
IF 0.975
Yuqiong Shi,Beibei Qiu,Xiangrong Wu,Yuxuan Wang,Jinhua Zhu,Xiuhua Liu,Dongbao Zhao
To extend the wide application range of luteolin (LUT), a slow-release drug delivery system is urgently needed. In this work, Fe 3 O 4 @ZIF-67 was reported as the carrier of LUT. A facile synthesis method of Fe 3 O 4 @ZIF-67 was established with simply continuous stirring. The structure and function of the nanocomposites were characterised by SEM, TEM, XRD, FTIR, VEM and TGA. Under the optimal reaction conditions, the maximum drug loading capacity of the carrier was obtained to be 308.07 µg/mg. And LUT was released about 55% from Fe 3 O 4 @ZIF-67 after 72 h. The adsorption kinetics and release kinetics of LUT were also investigated. The results showed that the relationship between time and drug loading fitted well with the quasi-second-order kinetic equation ( R 2 = 0.99) and the release mechanism followed the Sigmoidal model ( R 2 = 0.98). In short, Fe 3 O 4 @ZIF-67 could be developed as a carrier of the LUT delivery system for slow release.
研究领域:
药剂学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2019
·
IF 0
Shaokang Wang,Lingmeng Fu,Yi Wu,Hongmei Xiao,Jing Wang,Guiju Sun
The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis. The cells were exposed to different concentrations of luteolin (0, 40, 80, 120, 160, 200, 240 μM) for 24, 48, and 72 h respectively. The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay. Eca109 cells were then treated with luteolin (0, 40, 160, 240 μM) for 24 h. The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry (FCM). Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively. The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin. Further, the cell cycle was arrested in the S phase following treatment with luteolin. Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups, which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration. The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion, then inhibition. Therefore, luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3, caspase9 mRNA and protein. This study provides theoretical basis for further study and clinical application of luteolin. The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied.
研究领域:
肿瘤学
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细胞生物学
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药理学
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产品关联
DOI
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Juntong Yao,Yue Ma,Jinxin Liu,Shucheng Liu,Jianming Pan
Janus-like magnetic molecularly imprinted nanobottles (MMINBs) are designed by the twice modification of the bottle-like particles with 3-chloropropyl groups capped on exterior surface and amino-groups capped on interior surface for specific adsorption and separation of luteolin (LTL) which containing cis -diol groups. Firstly, silica nanobottles (NBs) derived from anisotropic emulsion containing three species of silane coupling agents (3-aminopropyltriethoxysilane, 3-chloropropyltrimethoxysilane, and tetraethyl orthosilicate) are prepared to pave for the subsequent modification. Then magnetic nanoparticles by thermal decomposition of ferric triacetylacetonate are attached to carboxylic acid functionalized interior surface of nanobottles. Imprinted polymers using 4-vinylphenylboronic acid as functional monomer are introduced onto exterior surface of magnetic nanobottles (MNBs) by atom-transfer radical polymerization strategy using activators regenerated by electron transfer (ARGET ATRP) reaction. The capture performances of as-prepared MMINBs are illustrated by rebinding equilibrium, kinetics, selectivity, and regeneration. The maximum adsorption amount of MMINBs is 187. 2 μmol g −1 at 308 K. Moreover, MMINBs shows feasible capture kinetics (120 min), excellent selectivity, and remarkable recyclability after 7 regeneration cycles (only 4.55% loss in adsorption amounts).
研究领域:
材料科学
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分析化学
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DOI
高影响因子
PHARMACEUTICAL RESEARCH
·
2013
·Vol. 31
·期 4
·847-860
·
IF 4.742
Tang Lan,Li Ye,Chen Wei-Ying,Zeng Shan,Dong Ling-Na,Peng Xiao-Juan,Jiang Wen,Hu Ming,Liu Zhong-Qiu
Purpose UDP-glucuronosyltransferases (UGTs) are responsible for the formation of glucuronides of polyphenolic flavonoids. This study investigated the UGT1A9-mediated glucuronidation of luteolin and the kinetics of luteolin glucuronide efflux. Method HeLa cells overexpressing UGT1A9 (HeLa-UGT1A9) were used to determine the kinetics of breast cancer resistance protein (BCRP)-mediated transport of luteolin glucuronides. Human UGT isoforms were used to determine glucuronidation rates. Results UGT1A9 was found to catalyze the production of four luteolin glucuronides, including three known monoglucuronides and a novel 3′, 4′-diglucuronide. Ko143, a potent specific inhibitor of BCRP, significantly inhibited efflux of luteolin monoglucuronides from HeLa1A9 cells and increased their intracellular levels in a dose-dependent manner. The formation of luteolin diglucuronide was observed when intracellular concentration of total monoglucuronides went above 0.07 nM. Conclusions Intracellular accumulation of diglucuronide was detected at high monoglucuronide concentrations (>0.07 nM). Diglucuronide production is speculated to be a compensatory pathway for luteolin disposition.
研究领域:
药理学
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分子生物学
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DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 21
·7342
·
IF 4.927
Meihua Xi,Yujie Hou,Ruolin Wang,Minhui Ji,Yingying Cai,Jingfang Ao,Heyu Shen,Mei Li,Jun Wang,Anwei Luo
Antibacterial hand sanitizers could play a prominent role in slowing down the spread and infection of hand bacterial pathogens; luteolin (LUT) is potentially useful as an antibacterial component. Therefore, this study elucidated the antibacterial mechanism of LUT againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus) and developed an antibacterial hand sanitizer. The results showed that LUT had excellent antibacterial activity against bothE. coli(minimum inhibitory concentration (MIC) = 312.5 μg/mL, minimal bactericidal concentration (MBC) = 625 μg/mL), andS. aureus(MIC = 312.5 μg/mL, MBC = 625 μg/mL). Furthermore, LUT induced cell dysfunction inE. coliandS. aureus, changed membrane permeability, and promoted the leakage of cellular contents. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) analysis showed that LUT treatment affected cell structure and disrupted cell membrane integrity. The Fourier transform infrared analysis (FTIR) also confirmed that the LUT acted on the cell membranes of bothE. coliandS. aureus. Overall, the application of LUT in hand sanitizer had better inhibition effects. Therefore, this study could provide insight into expanding the application of LUT in the hand sanitizer markets.Keywords:luteolin;E. coli;S. aureus;antibacterial activity;antibacterial hand sanitizer
研究领域:
药理学
·
微生物学
·
化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wenli Shi,Ruimin Ran,Xin Guo,Linjing He,Guoying Li
Despite its important role in reducing food waste and global hunger, preservation films exhibit poor environmental sustainability, mechanical protection and freshness-preserving capabilities. Herein, we proposed a sustainable in-situ rapid gelation strategy for fruit preservation. The core strategy involves sequentially encapsulating the natural antimicrobial and antioxidant luteolin (Lut) within liposome (Lip) and chitosan (CS) outer shell to construct core-double-shell structured nanoparticles. The outer shell confers excellent interfacial compatibility between Lut@Lip (LUL) and the CS substrate. Finally, rapid deprotonation cross-linking (70 s) forms a dense, porous gel structure. Lut was stably incorporated into the phospholipid bilayer of Lip through hydrophobic and hydrogen bonding interactions. This complex retained Lut's biological activity and the carrier advantages of Lip, demonstrating excellent physical stability, controlled release, antioxidant capacity (DPPH scavenging rate: 92.5 %; ABTS scavenging rate: 96.2 %), and favorable antibacterial properties. Density functional theory studies revealed that Lut achieved highly efficient electron transfer through the lowest HOMO-LUMO gap (ΔE = 2.6136 eV), playing a key role in antioxidant activity. Benefiting from the symbiotic reinforcement of LUL and CS, the gel coating demonstrated excellent water vapor and UV barrier properties, antifungal activity ( Botrytis cinerea inhibition rate: 86.9 %), and mechanical protection (reducing impact damage to cherries by 48.3 %). Cherries preservation experiment further validated the effectiveness of this gel coating. Under room temperature conditions (26–28 °C), the gel coating extended cherry shelf life by two-fold through reducing weight loss, preventing nutrient depletion, and inhibiting microbial growth. Additionally, compared to PE film, it reduced spoilage rates, carbon emissions, and costs by 63.6 %, 63.9 %, and 63.1 %, respectively, and achieved soil degradation within 21 days. This low-cost in-situ gelation technology offers a consumer-safe and scalable solution to reduce food waste, advance sustainable packaging, and alleviate plastic pollution.
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高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 8
·087511
·
IF 4.386
Wensi Xu,Xiansen He,Xiaokun Li,Suxiang Feng
In this study, a novel electrochemical sensor was designed to detect luteolin (Lu) with the composite of gold nanoparticles and nickel phthalocyanine-based 2D conductive metal-organic frameworks (Au NPs@NiPc-Cu MOFs) for the first time. The NiPc-Cu MOFs exhibit excellent conductivity, large specific surface area, and porous structure, which can accelerate the mass transfer process of target molecules. To further improve the sensitivity of the sensing platform, Au NPs with outstanding conductivity were introduced to the surface of NiPc-Cu MOFs to prepare Au NPs@NiPc-Cu MOFs. The synergistic effect of NiPc-Cu MOFs and Au NPs endows the sensor with excellent electrocatalytic performance and outstanding sensitivity. Under optimal conditions, the electrochemical sensor has a wide linear range (0.1–40 μM). Moreover, the prepared sensor possesses good stability and anti-interference ability. This method does not require complicated sample pretreatment, simple operation, and short detection time, which can provide a new method for the rapid detection of Lu.
研究领域:
分析化学
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材料科学
·
药学
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DOI
高影响因子
危险品化学品经营许可证（带存储）