ACS Applied Nano Materials
·
2025
·
IF 5.5
Meiqi Liu,Haonian Guo,Weiqiang Wang,Juanjuan Hou,Tingting Guo,Jinli Wang,Jianglong Mi,Quanlin Ma,Ziqiang Lei
The present study aimed to synthesize a green and nontoxic polymer emulsion for use as soil water erosion prevention materials. The P(nFe2O3-co-AA) polymer emulsion was then prepared by functionalizing iron oxide nanoparticles, preparing functional monomers, and copolymerizing these with acrylic acid. The water erosion resistance, compressive strength, and water retention of the emulsion-treated loess/red clay were investigated and germination tests were conducted. The results showed that the loess containing 0.50 wt % material resisted erosion for more than 416 h, which was 6250 times longer than for pure loess. During this time, the rate of water erosion was 0.03 ± 0.03%/h. Furthermore, the compressive strength of loess with the addition of 0.50 wt % material was 6.4 ± 0.2 MPa, which were 9.1 times higher than that of pure soil. The material demonstrated good water retention and showed no adverse effects on plant germination and growth, holding great promise for soil remediation applications.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 30
·7431-7440
·
IF 4.157
Yang Shirui,Zhang Wenxiang,Liu Zheyi,Zhai Ziyang,Hou Xudong,Wang Ping,Ge Guangbo,Wang Fangjun
Human serum albumin (HSA) is one of the most important serum carrier proteins that deliver small-molecule drugs to their specific targets. Clarifying the molecular mechanism of the interaction between natural HSA and drugs in an aqueous solution has been a hot topic in pharmaceutical chemistry, clinical medicine, and biochemistry in recent years, but it is still challenging. In this paper, the details of molecular interactions of HSA with a variety of therapeutic drugs including ibuprofen, indomethacin, phenylbutazone, and warfarin are systematically investigated using a mass spectrometry (MS)-based lysine reactivity profiling (LRP) strategy. The results reaffirm that the major ligand binding sites (including Sites I and II) of HSA are located in subdomains IIA and IIIA, while several potential drug-binding areas at subdomain IIIB and α helix IIB-IIIA are newly characterized. The MS-LRP strategy may have important application prospects in pharmacodynamics, pharmacokinetics, and safety evaluation of small-molecule drugs. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Chunling Nie,Ting Shen,Weicheng Hu,Qin Ma,Jiahui Zhang,Shuqian Hu,Huafeng Tian,Hao Wu,Xiaogang Luo,Jianguo Wang
In recent years, harmful microorganisms in water pose great harm to ecological environment and human health. To solve this problem, epsilon-poly- l -lysine (EPL) grafted cellulose beads were prepared via 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO) mediated oxidation and carbodiimide mediated cross-linking reaction. Hydroxyl groups on C6 of cellulose were oxidized to carboxyl groups by TEMPO and grafting reaction was achieved between newly formed carboxyl groups of cellulose and amino of EPL. The beads were characterized by FTIR, SEM, XRD and TGA. The crystalline form of cellulose transformed from cellulose I to cellulose II after being dissolved and regenerated. The grafted cellulose beads showed good antibacterial activities against Gram-negative Escherichia coli , Gram-positive Staphylococcus aureus and Alicyclobacillus acidoterrestris with 10 h. The beads could be biodegraded in soil after 28 days. It is expected that the bio-based composite beads could have potential applications in water purification and food treatment fields.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Yanli Wang,Wangchong Ji,Yongjing Xu,Liming Zou,Hongwei Lu,Chenkai Sun
Hyperbranched poly(phenylalanine-lysine) (HBP(P-L)) was synthesized by lysine, phenylalanine and N, N-dimethylbisacrylamide (MBA) via Michael addition reaction and characterized by Fourier transform infrared (FTIR) spectra, 13 CNMR and 1 HNMR. Furthermore, fluorescence spectroscopy indicates the fluorescence intensity of HBP(P-L) aqueous solutions increases with the concentration of HBP(P-L) increasing; Multiwalled carbon nanotubes (MWCNTs) were modified using HBP(P-L) for the purpose of preparing MWCNTs-HBP(P-L), which was characterized by FTIR, Raman analysis and thermogravimetric analysis (TGA). Visual inspection method, UV–vis absorption spectra and Zeta potential are adopted to evaluate the dispersion properties of MWCNT-HBP(P-L) aqueous solutions, and the results illustrate that HBP(P-L) does improve the dispersibility of MWCNT. Moreover, MWCNT-HBP(P-L) owns the best dispersibility in aqueous solution when the mass ratio is 1:60. Additionally, it presents an excellent linear relationship between the fluorescence intensity of MWCNTs-HBP(P-L) dispersion and the mass ratio of MWCNT-HBP(P-L). MWCNTs-HBP(P-L) exhibits unique dispersion properties as well as excellent fluorescence properties, which make the MWCNT-HBP(P-L) be potentially used in the field of optical materials.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Guoguang Wei,Yan Chen,Ning Zhou,Qiuhao Lu,Sheng Xu,Alei Zhang,Kequan Chen,Pingkai Ouyang
Enzyme cofactors are essential in biocatalysis for many enzymes. However, their high commercial cost limits the industrial application of cofactor-dependent enzymes. We overcame this challenge by constructing a self-sufficient biocatalyst that co-immobilised pyridoxal 5′-phosphate (PLP) and L-lysine decarboxylase (LDC) using natural polymer chitin as the functional material. First, degree of deacetylation (DDA) of chitin was regulated to enhance PLP adsorption and immobilisation. Then, the enzyme was immobilised via fusion of a chitin-binding domain. Chitin with 36% DDA efficiently co-immobilised LDC and PLP. Laser scanning confocal microscope and scanning electron microscope proved that the LDC and PLP were immobilised on the chitin. The self-sufficient biocatalyst retained over 55% of its original activity after five cycles. The proposed strategy provides a new way to achieve co-immobilisation of PLP-dependent enzymes.
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2018
·Vol. 37
·期 1
·36-42
·
IF 2.016
Pan Wei,Yin Dong-Xiao,Jing Hai-Rong,Chang Hao-Jing,Wen Hao,Liang De-Hai
The structure and kinetics of the complex formed by hyaluronic acid (HA) and poly(L-lysine) (PLL) were studied by time-resolved laser light scattering, TEM, and AFM. Because HA has a hydrophilic backbone, the complexes formed by HA and PLL are different from those formed by oppositely charged polyelectrolytes both having hydrophobic backbones. Instead of forming strong aggregates and even precipitates, the complex in the presence of excess HA is stable in the studied time period. More importantly, the complex spontaneously forms core-corona structure by the rearrangement of HA chains. The core is composed of complex rich of PLL and the corona is mainly HA. Further analysis shows that the hydrogen bond formed by HA creates a barrier hindering the further relaxation of HA chains. The automatic formation of core-corona structure by PLL/HA is helpful not only to understand the relaxation of polyelectrolyte in complex, but also to develop drug carriers with desirable properties.
研究领域:
生物化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Kai Wang,Weiwei Li,Jiaming Chen,Ying Peng,Jiang Zheng
Many furan-containing compounds are known to be toxic and/or carcinogenic . Metabolic activation of toxic furans to cis -enediones ( cis -enedials or γ -ketoenals) is generally considered as the initial step towards the processes of their toxicities. Sequential modification of key proteins by the electrophilic reactive intermediates is suggested to be an important mechanism of the toxic actions. In the present study, we developed a novel and simple analytical platform to detect protein modification resulting from metabolic activation of model compound 2,5-dimethylfuran (DMF). 4-Bromobenzylamine and 4-bromobenzylmercaptan were employed to trap protein adductions at cysteine and lysine residues, respectively. The resulting protein samples were proteolytically digested by chymotrypsin and Pronase E, followed by LC–MS/MS analysis. Modifications of cysteine and lysine residues of proteins were observed in microsomal incubations and animals after exposure to DMF. In conclusion, the approach established has been proven highly selective and reliable. This advance allows us not only to detect the protein adductions but also to define the structural identities of amino acid residues modified. This technique provides a unique platform to assess protein modifications arising from metabolic activation of potentially harmful furan-containing compounds. Hepatic protein adductions were found to be proportional to the hepatotoxicity of DMF.
研究领域:
毒理学
·
蛋白质组学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2017
·Vol. 142
·期 24
·4773-4781
·
IF 3.885
Haijiao Zheng,Naizhong Song,Xiqian Li,Qiong Jia
Plasminogen (Plg) is a kind of glycoprotein which plays an important role in cell migration. The determination of Plg content can directly reflect the abnormal manifestation of fibrinolytic system dysfunctions. In the present work, lysine (Lys)-based adsorbents were prepared for the specific capture of Plg through the covalent binding of Lys with a polymer monolithic substrate. Lys was modified with β-cyclodextrin (β-CD) via a click reaction and anchored to the substrate with biotin by host–guest interaction. The biotin-Lys-CD based monolithic material was employed for the specific capture of Plg. Combining with mass spectrometry (MS) determinations, the method exhibited a low detection limit of 1.0 fmol with relative standard deviations below 10.0% for Plg. Considering the advantages of simplicity, sensitivity, and high specificity, the developed approach was successfully applied to the determination of Plg in human plasma samples and opened a gallery for testing Plg as a biomarker for the diagnosis of fibrinolytic system dysfunctions.
研究领域:
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 18
·e202400521
·
IF 2.8
Yufeng Shen,Rui Jin,Feifang Zhang,Bingcheng Yang
The high hydrophobicity and chemical inertness of poly(styrene-divinylbenzene) (PS-DVB) microspheres make their surface hydrophilic modification difficult. Here we describe a facile way to convert PS-DVB microspheres to hydrophilic, then can be used as polar stationary phase for hydrophilic interaction chromatography. This approach utilizes the grafting of an acrylamide-terminated lysine zwitterionic monomer onto PS-DVB microspheres via free radical polymerization. The obtained stationary phase shows good hydrophilicity and a typical retention mechanism of hydrophilic interaction chromatography toward several model polar analytes. It also exhibits obvious zwitterionic properties and is capable of separating cationic and anionic analytes simultaneously. The column shows negligible bleeding level, much superior to silica-based ones.
研究领域:
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2013
·Vol. 32
·期 1
·44-50
·
IF 0.917
Haichao Xu,Yafei Luan,Zhaoqiang Wu,Xinming Li,Yuling Yuan,Xiaoli Liu,Lin Yuan,Dan Li,Hong Chen
A novel biomaterial based on polyurethane (PU) was prepared through physical incorporation of lysine-containing copolymer to improve its hemocompatibility and surface recognition of plasminogen. The lysine-containing copolymer was synthesized via the copolymerization of 2-ethylhexyl methacrylate (EHMA), oligo (ethylene glycol) methyl ether methacrylate (OEGMA) and 6- tert -butoxycarbonyl amino-2-(2-methyl-acryloylamino)-hexanoic acid tert -butyl ester (Lys(P)MA), followed by the deprotection of COOH and ε -NH 2 groups on lysine residues in the copolymer. The composition of the copolymer can be adjusted by varying the monomer feed ratio. The three components contribute to improving the compatibility with PU, resistance to nonspecific protein adsorption and specific binding of plasminogen, respectively. The binding capacity towards plasminogen increased with the lysine content in the copolymer. This approach illustrates a simple way for the generation of novel biomaterials with improved hemocompatibility and surface recognition of specific biomolecules.
研究领域:
生物材料
·
医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Sun Ping,Zhou Yue,Qiu Tong,Peng Jian
Laccase is an enzyme known for its eco-friendly uses in environmental cleanup and biotechnology. However, it has limitations such as low stability, high cost, and complex recycling. So, there is a need for laccase mimics that can effectively imitate its properties. Herein, we created copper formate–lysine nanoparticles (Cuf-Lys) that mimic laccase’s activity. The developed Cuf-Lys demonstrated remarkable polyphenol oxidase-like activity, stability, and recyclability, making them suitable for the fabrication of efficient colorimetric sensors for the detection of epinephrine. These sensors had a specific response and could accurately measure epinephrine concentrations ranging from 2.5 to 50 μM, with a detection limit as low as 1 μM. Furthermore, the biosensor demonstrated high sensitivity and selectivity when applied to the detection of rutin. The limit of detection for rutin was determined to be 0.16 μM while in the linear concentration range of 0.25 to 150.0 μM. We believe that Cuf-Lys provide a new route for the design of laccase mimics, showing potential applications for biomedical diagnosis and environmental monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Polymeric Materials and Polymeric Biomaterials
·
2022
·
IF 3.221
Zhao Wang,Wenli Song,Ruilong Sheng,Xinyu Guo,Lingyun Hao,Xiaojuan Zhang
A new series of cholesterol-bearing poly(methacrylate)s with pendant cationic (l-)-lysine (Chol-PHML) was synthesized via reversible addition–fragmentation chain transfer (RAFT) polymerization, followed by Boc-deprotection. Their structures were characterized by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Then, the average particle sizes and zeta potentials of the Chol-PHML/pDNA polyplexes were examined using dynamic light scattering (DLS), and their plasmid DNA (pDNA)-binding ability was analyzed using agarose gel retardation assay. In vitro cytotoxicity of the polycations was determined using a cell counting kit-8 (CCK-8) assay and lactate dehydrogenase (LDH) kits in HeLa cells. The polyplex’s gene transfection effectiveness was investigated using a luciferase assay. The results revealed that the Chol-PHML series could efficiently bind/load pDNA with a high binding affinity (N/P < 3.0) and subsequently form nanoparticles with the sizes of 90–200 nm and zeta potentials of ∼+40 mV in an aqueous solution. The in vitro cytotoxicity and gene transfection efficiency considerably depended on the Chol-PHML vector’s molecular weight. The optimal Chol-PHML27 exhibited a substantially higher gene transfection efficiency and lower cytotoxicity than those of the linear structural PLL (15 K–30K) and PHML26 control. This study presents a simple method for synthesizing biocompatible and controllable cationic gene vectors by introducing hydrophobic cholesterol moieties, which will function as a promising foundation for gene delivery.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）