FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Hedi Wen,Deju Zhang,Zhenzhen Ning,Zihao Li,Yan Zhang,Jingbo Liu,Ting Zhang
In order to explore the effect of polyphenol structure on the foaming properties of protein, the ovalbumin-polyphenol (OVA-polyphenol) complexes were developed by combining OVA with gallic acid (GA), 3,4-dihydroxybenzoic acid (DA), P-hydroxybenzoic acid (PA), 4-coumaric acid (CA), trans -3-hydroxycinnamic acid (T3A) and trans -2-hydroxycinnamic acid (T2A), respectively, and then the foaming properties, binding interactions, physicochemical properties and protein structure of complexes were determined. Results showed that the foaming ability (FA) and surface hydrophobicity were in the order of OVA-PA > OVA-T2A > OVA-DA > OVA-CA > OVA-T3A > OVA-GA > OVA, whereas the α-helix, fluorescence quenching ability and ζ-potential of OVA-polyphenol complexes followed an opposite trend, suggesting that fewer hydroxyl groups and ortho-aromatic structures were positively associated with higher foaming properties of the complexes. Physicochemical properties revealed that OVA-polyphenol complexes obtained higher ζ-potential and surface hydrophobicity, revealing that the addition of polyphenol promoted the adsorption and rearrangement of OVA in liquid-gas interface and increased its foaming properties. Molecular interactions demonstrated that PA, due to their fewer hydroxyl binding sites, and T2A, due to the existence of competitive binding, led to the instability of OVA-polyphenol complexes, promoting the movement of OVA and the exposure of its hydrophobic groups, thus contributing to the conversion of α-helix to the random coil conformations and ultimately promoting the FA of the complexes. In conclusion, polyphenols could increase the foaming properties of OVA, and PA retained the highest priority due to its excellent overall performance. These findings will be of interest for developing foods with improved functional properties.
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JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2023
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IF 10.9
Yike Hou,Zhe Tang,Jabeen Farheen,Madiha Saeed,Lijia Luo,Wenzi Ren,Dandan Luo,Asim Mushtaq,Ruibo Zhao,Jian Ge,Zhangsen Yu,Yao Li,M. Zubair Iqbal,Xiangdong Kong
Vaccine-based cancer immunotherapy has demonstrated a significant potential for cancer treatment in clinics. Although the efficiencies of vaccines are limited, they can be enhanced by a well-designed antigen delivery system that promotes sufficient antigen presentation of dendritic cells (DCs) for initiating high T cell immunity. Herein, antigen-loaded manganese oxide (Mn 3 O 4 ) triangular-shaped ultrasmall nanoparticles were prepared to stimulate DC-based immunotherapy under the guidance of T 1 magnetic resonance imaging. The FDA-approved triblock copolymer Pluronic® F-68 was used not only to transfer the phase from hydrophobic to hydrophilic but also to enrich antigen loading and improve the biocompatibility of the prepared nanoparticles. Ovalbumin (OVA), a model antigen, was adsorbed on the surface of polymer-coated nanoparticles through electrostatic interaction to form Mn 3 O 4 @PF68-OVA nanoparticle-antigen complexes to stimulate DC-based immunization and antigen-specific T cell immunity. The Mn 3 O 4 @PF68-OVA nanovaccine (NV) induces negligible toxicity effects against 4T1 and bone marrow-derived dendritic cells (BMDCs) by conventional methods supports the proliferation of intestine organoids, which are an innovative three-dimensional cytotoxicity evaluation system, thereby indicating their potential safety for in vivo cancer therapies. The designed paramagnetic nanovaccine possessed excellent OVA delivery to dendritic-regulated antigen-specific T cells in vitro by stimulating the maturation level of BMDCs. In addition, Mn 3 O 4 @PF68-OVA NVs enhance immunity in vivo by increasing the T-cells and M1 macrophages, which suggests improved immunity. Excitingly, vaccination with Mn 3 O 4 @PF68-OVA offer complete protection in the prophylactic group and significant tumor inhibition in the therapeutic group against B16-OVA tumor. In addition, the designed nanovaccine demonstrated high T 1 -MR imaging in the tumor, further justifying enhanced tumor accumulation and capability to real-time monitor the treatment procedure. This study presents a promising nanosystem to design an effective nanovaccine for T 1 -MR imaging-guided tumor immunotherapy.
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PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2023
·
IF 2.7
Yike Hou,Shuqi Huang,Zhe Tang,Zhangsen Yu,Muhammad Zubair Iqbal,Xiangdong Kong
Photo-responsive combinational therapeutics attained significant attention in cancer theranostic owing to the versatile features of nanomaterials. The simplest nano-design with unique properties to carry out multifunctionalties is a challenging assignment. Herein, a facile solvothermal method is employed to fabricate homogeneous MnO 2 sea urchin-shaped nanoparticles (MnO 2 -OVA NPs) with a high loading capacity (86.15%) of ovalbumin (OVA) antigen. The prepared MnO 2 -OVA NPs demonstrated excellent biocompatibility against different cell lines and significant cellular uptake, resulting in mature dendritic cells to enhance anti-tumor immunity. The synthesized sea urchin-shaped MnO 2 -OVA NPs further exhibited self-generated catalytic properties that efficiently relieve tumor hypoxia and improve cancer therapies. More excitingly, MnO 2 -OVA NPs endorsed reactive oxygen species (ROS) in response of minute UV irradiation (mW) for photodynamic therapy. Further, the prepared photo-responsive nanoparticles showed promising T 1 -MR imaging and relaxivity properties for diagnosis and real-time drug delivery monitoring. These synergetic therapeutic strategies produced by MnO 2 -OVA NPs showed excellency as a potential theranostic agent.
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FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Hanyu Li,Minghui Zhao,Sijie Zhou,Huajiang Zhang,Jing Wang,Ning Xia,Yujia Liu,Shihui Hua,Guixin Tan
Curcumin is a hydrophobic polyphenol with various health benefits, but its poor water solubility and stability limit its application in the food and pharmaceutical industries. In this study, a pH-driven method was developed to prepare ovalbumin (OVA)-curcumin nanocomplexes without organic solvents, reducing the environmental impact. The results showed that curcumin was encapsulated in OVA with a high efficiency of over 95% and remained in an amorphous state within the protein molecules. Molecular docking and spectroscopic analyses revealed that curcumin was mainly bound and stabilized by hydrogen bonds and hydrophobic interactions in the hydrophobic core of OVA. The binding strength of the intermediate stage of the pH-driven method was stronger than that of the final stage, due to the exposure of OVA molecular binding sites and the involvement of electrostatic attraction. Furthermore, the potential of polysaccharide blending to enhance the stability of the nanocomplexes was investigated. The results showed that sodium alginate effectively enhanced the photostability and thermal stability of the OVA-curcumin nanocomplexes by co-solubilization and electrostatic complex formation. In addition, simulated in vitro digestion also demonstrated that the pH-driven method combined with polysaccharide blending increased the bioaccessibility of curcumin in nanocomplexes from 17.79% to 52.54%. Therefore, the pH-driven method coupled with polysaccharide blending ensures the encapsulation and stabilization of hydrophobic bioactive substances for use in their delivery systems to improve bioaccessibility.
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FOOD CHEMISTRY
·
2022
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IF 9.231
Huimin Hao,Jian Zhu,Bin Yang,Li Peng,Shuyan Lou
Pesticide carbaryl can cause serious environmental pollution and its sensitive detection is of increasing interest. Gold nanoparticles (AuNPs) are classically colorimetric probes for detection of many analytes, but the instability in complex media limits their application. Here, Au@Ova NPs have been developed as a stable, effective, sensitive, and selective sensing system for colorimetric detection of carbaryl. Au@Ova NPs present unique and proper colloidal stability in various medias containing salt, small molecules, organic solvent (DMSO), and seawater, which are distinct from previous ones including citrate (or rhodamine B) capped AuNPs. Compared with Au@BSA NPs, Au@Ova NPs showed efficient responses to carbaryl by inhibiting acetylcholinesterase, with a linear concentration range between 0 and 25 μg/L and a detection limit of 0.25 μg/L. In addition, this nanoprobe also has good selectivity and can be applied in different real samples analysis, including fruit juice (tomato and apple) and real water samples (artificial urine and seawater).
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Food Science and Human Wellness
·
2022
·
IF 8.022
Yu Ma,Ming Liu,Donghui Li,Jie Li,Zixin Guo,Yunjun Liu,Shengnan Wan,Yixiang Liu
The numerous health benefits of olive oil are widely known, however, it also provides anti-allergic properties that have not yet been fully defined. In this study, the anti-allergic activity of olive oil was evaluated by analyzing the clinical symptoms and immune-related factors in BALB/c mice that had ingested 600 mg/(kg∙day) olive oil for two weeks prior to the evaluation. An allergy model was subsequently constructed for analysis, the results of which showed that the olive oil reduced the scores of allergic symptoms in the mice, and up-regulated the hypothermia and the decline in the immune organ index. Moreover, fewer allergy-related cytokines and reduced intestinal inflammation was discovered in the olive oil-treated group. In addition, analysis of intestinal mucosal immune-related factors revealed that the olive oil promoted the expression of intestinal tight junction proteins (Claudin-1, Occludin, and ZO-1) and IL-22, and helped maintain the integrity of the intestinal epithelial physical barrier. Increased levels of mucin 2 and β -defensin were also found in the intestinal mucus of the olive oil-treated mice. These findings suggest that the oral administration of olive oil effectively attenuated the ovalbumin-induced allergic immune response in the mice, and had a positive effect on intestinal epithelial mucosal immunity.
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CARBOHYDRATE POLYMERS
·
2022
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IF 10.723
Lihua Li,Xinyu Sun,Hui Zhang,Mengna Dong,Jiao Wang,Shuang Zhao,Minghui Shang,Xin Wang,Hui Zhangsun,Li Wang
Nano-delivery systems play an important role in the development of nutritional supplements due to their efficient encapsulation and delivery properties for nutrients. Herein, we prepared protein-polysaccharide nanoparticles as a novel amphiphilic nano-delivery system based on gallic acid modified chitosan (GCS) and ovalbumin (OVA) by pH-driven and calcium ion crosslinking. The nanoparticles loaded with hydrophilic riboflavin (Rib) and hydrophobic quercetin (Que) as nutrient models were abbreviated as GCS-OVA-Rib NPs and GCS-OVA-Que NPs, respectively. Their encapsulation efficiencies for Rib and Que. were 66.36 % and 96.61 %, respectively. In addition, GCS-OVA-Rib NPs and GCS-OVA-Que NPs showed antioxidant activity as well as good stability and delivery capacity for Rib and Que. in simulated digestion with release ratios of 78.38 % and 84.15 %, respectively. More importantly, GCS-OVA-Rib/Que. NPs performed good biocompatibility for further applications. Overall, this work provides some useful insights for the design of novel amphiphilic nano-delivery systems based on polysaccharides and proteins.
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Innovative Food Science & Emerging Technologies
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2022
·
IF 7.104
Yue Chen,Ning Qiu,Bin Ma,Gan Hu,Deog-Hwan Oh,Xing Fu
Hydrogels synthesized from natural proteins have better biocompatibility than polymer hydrogels. In this paper, the hydrogels (referred to as tOVA/ [email protected] ) was developed using coordinative crosslinking of thiolated ovalbumin (tOVA) and thiolated gelatin (tGel) with silver nitrate . After thiolation with Traut's reagent, the free sulfhydryl group content of ovalbumin increased from 1.5 μmol/g to 68.6 μmol/g, and the molecular weight (MW) increased from 44.5 kDa to around 50 kDa. Then, the physicochemical and rheological properties of hydrogels with different tOVA/tGel ratios (10:0, 7:3, 5:5, 3:7, and 0:10) were analyzed. With the decrease in the proportion of tOVA/tGel, the scanning electron microscope (SEM) showed that the network structure of hydrogel became more uniform and denser. Meanwhile, microrheological analysis displayed that the gel properties had been improved, which coincidently confirmed a better water-holding performance. During the antibacterial experiment, the diameter of the bacteriostatic ring of tOVA/ [email protected] hydrogels against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ) could reach 10.8 mm and 10.3 mm respectively, indicating their significant antibacterial properties . In summary, hydrogel prepared with thiolated proteins exhibited a good three-dimensional (3D) network structure with the help of silver ions, which was expected to play an important role in the field of food and medicine as antibacterial materials.
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MICROCHEMICAL JOURNAL
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2022
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IF 5.304
Sheng Bi,Xue Yang,Baoyue Zhang,Yukui Tong,Feng Zhang,Miaomiao Tian
In this study, ZIF-based metal oxide molecularly imprinted polymers (ZIF@PEI@TiO 2 -MIPs) were prepared for efficient and selective separation and enrichment of ovalbumin (OVA) in egg white samples. The effects of pH, OVA concentration, ionic strength, and elution type on the adsorption process were investigated. ZIF@PEI@TiO 2 -MIPs have rich imprinted sites and large specific surface area. PEI provides a large amount of amino to bind TiO 2 . As a new type of solid phase extraction adsorbent, ZIF@PEI@TiO 2 -MIPs can rapidly adsorb OVA and have a large adsorption capacity. The adsorption time is 35 min, and the maximum adsorption capacity is 417.6 mg g −1 . The imprinted sites of adsorbent can distinguish the template protein (OVA) from interference proteins, indicating that ZIF@PEI@TiO 2 -MIPs have good selectivity and adsorption specificity, and the adsorption model conforms to Langmuir adsorption model and pseudo-second-order kinetics. The adsorbent still has good adsorption performance after six adsorption and elution cycles with 0.5% cetyltrimethylammonium bromide (CTAB) as the eluent, indicating its good stability. In conclusion, ZIF@PEI@TiO 2 -MIPs can specifically identify OVA in complex samples, providing a large possibility for the application of glycoprotein in activities such as biology and clinical practice.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Zhenguang Liu,Haiyu Ni,Lin Yu,Shuwen Xu,Ruonan Bo,Tianxin Qiu,Pengfei Gu,TianYu Zhu,Jin He,Adelijiang Wusiman,Shaowu Zhu,Jiaguo Liu,Yuanliang Hu,Deyun Wang
Recently, the cubosomes have been widely studied as drug carriers. It has been described that cubosomes could further stimulate the immune response after carrying the immune enhancer. Polygonatum sibiricum polysaccharide (PSP), one of the most important biologically active ingredients of Polygonatum sibiricum , has been reported as an immunostimulant to improve immune responses. This study was aimed to observe the immunomodulation effects of ovalbumin (OVA) absorbed cetyltrimethylammonium bromide-modified Polygonatum sibiricum polysaccharide cubosomes (CTAB-modified PSP-Cubs/OVA). Firstly, the antigen uptake of CTAB-modified PSP-Cubs/OVA by macrophages was determined in vitro. After that, mice were immunized with CTAB-modified PSP-Cubs/OVA. The activation of dendritic cells in lymph nodes, activation of lymphocyte, ratios of CD4 + to CD8 + , the concentrations of OVA-specific IgG in serum and the cytokines concentrations were analyzed. As the results showed, CTAB-modified PSP-Cubs/OVA could promote the production of OVA-specific IgG in serum. The ratio of CD4 + to CD8 + in CTAB-modified PSP-Cubs/OVA group was significantly increased compared with other groups. CTAB-modified PSP-Cubs/OVA could significantly activate dendritic cells and promote lymphocyte proliferation. The results indicated that CTAB-modified PSP-Cubs/OVA could promote the secretion of related cytokines and the proliferation of lymphocytes, stimulate the cellular immune response and increase the level of humoral immunity. Above all, CTAB-modified PSP-Cubs had good adjuvant activity.
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Pharmaceutics
·
2021
·Vol. 13
·期 9
·1384
·
IF 6.321
Haibo Feng,Jie Yang,Hui Zhi,Xin Hu,Yan Yang,Linzi Zhang,Qianqian Liu,Yangyang Feng,Daiyan Wu,Hangyu Li
In this investigation, to maximize the desired immunoenhancement effects of PsEUL and stimulate an efficient humoral and cellular immune response against an antigen, PsEUL and the model antigen ovalbumin (OVA) were coupled using the N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) reaction to yield a novel delivery system (PsEUL-OVA). The physicochemical characteristics and immune regulation effects of this new system were investigated. We found the yield of this EDC method to be 46.25%. In vitro, PsEUL-OVA (200 μg mL−1) could enhance macrophage proliferation and increase their phagocytic efficiency. In vivo, PsEUL-OVA could significantly increase the levels of OVA-specific antibody (IgG, IgG1, IgG2a, and IgG2b) titers and cytokine (IL-2, IL-4, IL-6, IFN-γ) levels. Additionally, it could activate T lymphocytes and facilitate the maturation of dendritic cells (DCs). These findings collectively suggested that PsEUL-OVA induced humoral and cellular immune responses by promoting the phagocytic activity of macrophages and DCs. Taken together, these results revealed that PsEUL-OVA had the potential to improve immune responses and provide a promising theoretical basis for the design of a novel delivery system.Keywords:polysaccharides fromEucommia ulmoides;adjuvant;delivery system;macrophage;dendritic cell
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2019
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IF 0.931
Zheng CHE,Shi-Yu YUE,Nan WU,Jia-Wei XU,Qing CHEN
Tungstotellurate(VI)-coated magnetic nanoparticles (MNP) were prepared by immobilization of tungstotellurate(VI) salt onto the surface of branched polyethyleneimine (PEI)-modified magnetic nanoparticles via electrostatic interaction. The structure and composition of TeW@MNP were confirmed by characterization with FT-IR, EDS and SEM. The obtained TeW@MNP composite had proven to be a promising adsorbent for the adsorption of ovalbumin in egg whites. Approximately 0.5 mg of TeW@MNP composite gave rise to a maximum adsorption efficiency of 91.6% for 100 μg mL –1 ovalbumin in 200 mL of sample solution at pH 4.0 (BR buffer). The retained ovalbumin was readily recovered by using 0.1% SDS as a stripping reagent, providing a recovery of 98.1%. The adsorption behavior of ovalbumin fitted Langmuir model, corresponding to a theoretical adsorption capacity of 373 mg g –1 . The TeW@MNP composite was practically applied to the selective isolation of ovalbumin from egg whites, and SDS-PAGE assay results clearly indicated that the ovalbumin with high purity was obtained. The above experimental results illustrated the great application potentials of polyoxometalates for protein isolation and purification and extended the application scope of polyoxometalates in life science field.
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