JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2012
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IF 2.888
Li Jiang,Jundong Dai,Zhenlin Huang,Qinghua Du,Jiahao Lin,Yurong Wang
Gastrodin (Gas) and puerarin (Pur) are bioactive substances derived from traditional Chinese medicine Gastrodia elata and Radix Puerariae , respectively, which were often used together in Chinese clinical prescriptions. Their injections were used in combined way for treatment of some cardiocerebrovascular diseases in clinic, especially for vertigo due to vertebrobasilar ischemia. In this paper, interaction of gastrodin and puerarin in rat plasma pharmacokinetics via intragastic ( i.g. )/intravenous ( i.v. ) administration was investigated. A reliable HPLC method was developed for simultaneous determination of Gas and Pur in rat plasma with a linear range of 0.101–101 μg/mL for Gas and 0.0500–5.98 μg/mL for Pur ( r 2 > 0.993). The LLOQ, LOD of Gas and Pur were determined to be 0.101, 0.0486 μg/mL, and 0.05, 0.0245 μg/mL, respectively. The intra-day and inter-day precision were all less than 12.0%, whilst the accuracy were all within 96.4 ± 6.00%. The proposed method has been successfully applied to the pharmacokinetic study of the analytes in rats after i.g. / i.v. administration of Gas and Pur alone or combined with each other ( i.g. : 40 mg/kg Gas, 400 mg/kg Pur; i.v. : 20 mg/kg Gas, 20 mg/kg Pur). Blood samples were collected from retinal vein plexus of rats at predetermined time points and plasma containing the internal standard tyrosol (IS) were precipitated by methanol and chromatography was carried out on a C 18 column with a gradient mobile phase of ACN–H 2 O with 0.05% phosphoric acid as a modifier. The pharmacokinetic profiles of combined administration were found to be distinct from those of given alone. The C max , T max , T 1/2 , MRT of Gas administrated alone or combined with Pur via i.g. were 21.7 μg/mL, 0.250 h, 2.81 h, 0.830 h and 18.4 μg/mL, 0.550 h, 0.970 h, 1.37 h, respectively, of Pur administrated alone or combined with Gas via i.g. were 0.490 μg/mL, 1.95 h, 1.33 h, 2.10 h and 2.01 μg/mL, 0.570 h, 4.00 h, 5.10 h, respectively. The relative oral bioavailability of Pur in combined administration was 10.7 times as much as that of single administration, whilst 1.52 folds in Gas. These results indicate that co-administration of Gas and Pur is a promising combination to gain higher bioavailability and it is suggested that doctors pay more attention to the dosages of the two when simultaneously using both of them.
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高影响因子
PHYTOTHERAPY RESEARCH
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2026
·
IF 8.1
Long Cheng,Minghong Gan,Huiyang Wang,Yanru Song,Yang Bai,Dongfang Zhang
Type 2 diabetes (T2D) is a chronic disease that seriously threatens human health. Puerarin, the primary bioactive component of Pueraria lobata , alleviates T2D through multiple mechanisms, including protection of pancreatic β-cells, enhancement of insulin sensitivity, and antioxidant effects. However, its specific mechanisms of action against T2D remain incompletely understood. This study aimed to investigate the curative effects of puerarin in ameliorating glucose and lipid metabolism disorders in T2D mice by preserving gut microbiota homeostasis and promoting adipose tissue thermogenesis. To achieve this, 16S rRNA gene sequencing and metabolomics were employed to assess its effects on gut microbiota and fecal metabolites, while qRT-PCR and western blot were conducted to evaluate its impact on the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/peroxisome proliferator-activated receptor γ (PPARγ) signaling pathway. The results demonstrated that puerarin promoted gut microbiota homeostasis and elevated butyrate levels. Specifically, it increased the abundance of beneficial bacteria (Lactobacillaceae, Muribaculaceae, and Akkermansiaceae) and reduced the abundance of harmful bacteria (Desulfovibrionaceae, Marinifilaceae, and Helicobacteraceae). Moreover, puerarin enhanced brown adipose tissue (BAT) activity via the PI3K/AKT pathway and induced a browning phenotype in white adipose tissue (WAT) through the PPARγ pathway. These effects promoted mitochondrial biosynthesis, lipolysis, glucose utilization, and energy expenditure. Puerarin also increased glucagon-like peptide-1 (GLP-1) secretion, thereby further improving insulin sensitivity. In conclusion, this study confirms that puerarin improves glucose and lipid metabolism disorders in T2D mice by regulating gut microbiota homeostasis and promoting adipose tissue thermogenesis, which holds considerable potential for the treatment of T2D.
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2026
·Vol. 70
·期 18
·e70603
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IF 4.5
Xiang Zhou,Hao Chen,Changjian Xue,Jiayao Sun,Yukun Ma,Xiang Ma,Chenxi Wu,Jing Shi,Wei Chen,Hongliang Qian,Dechun Huang
Medicinal and edible plants are promising antidiabetic candidates due to their combined nutritional and pharmacological properties. However, the disconnection between bioactive substance extraction and efficacy validation hampers the development of integrated functional interventions. Here, a green and efficient subcritical ethanol-water extraction (SEWE) system was developed to obtain puerarin-enriched extracts (PEE) from Pueraria lobata . By introducing ethanol as a green polarity-modifying co-solvent, the SEWE system enabled polarity-tunable extraction under relatively mild subcritical conditions, thereby balancing extraction efficiency and compound stability. Compared with conventional extraction methods, SEWE significantly enhanced puerarin enrichment and overall extract quality, and the resulting extracts (SEWE-P) further exhibited enhanced bioactivities, including antioxidant, enzyme inhibitory, and anti-inflammatory effects, together with intracellular ROS-scavenging capacity, while maintaining good biocompatibility in vitro. In streptozotocin-induced diabetic mice, oral administration of SEWE-P significantly improved glycemic control, enhanced insulin sensitivity, and promoted pancreatic β-cell recovery. Mechanistically, SEWE-P activated the PI3K/AKT signaling pathway to improve glucose metabolism and suppressed NF-κB-mediated inflammation and oxidative stress. Overall, this study establishes an integrated green extraction–function evaluation strategy, providing a scalable approach for the development of multifunctional plant-based antidiabetic interventions.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2023
·
IF 5
Shuang Zhou,Yuhua Li,Yi Hong,Zhitao Zhong,Min Zhao
Puerarin (Pue), an isoflavone compound extracted from Pueraria, has been shown to inhibit inflammation and reduce cerebral edema. The neuroprotective effect of puerarin has attracted much attention in recent years. Sepsis-associated encephalopathy (SAE) is a serious complication of sepsis that causes damage to the nervous system. This study aimed to investigate the effect of puerarin on SAE and elucidate the potential underlying mechanisms. A rat model of SAE was established by cecal ligation and puncture, and puerarin was injected intraperitoneally immediately after the operation. Puerarin was found to improve the survival rate and neurobehavioral score of SAE rats, alleviate symptoms, inhibit the level of brain injury markers NSE and S100β, and improve the pathological changes in rat brain tissue. Puerarin was also found to inhibit the level of factors related to the classical pathway of pyroptosis, such as NLRP3, Caspase-1, GSDMD, ASC, IL-1β, and IL-18. Puerarin also reduced the brain water content and penetration of Evan’s Blue dye in SAE rats, and reduced the expression of MMP-9. In the in vitro experiments, we further confirmed the inhibitory effect of puerarin on neuronal pyroptosis by establishing a pyroptosis model in HT22 cells. Our findings suggest that puerarin may improve SAE by inhibiting the classical pathway of NLRP3/Caspase-1/GSDMD-mediated pyroptosis and reducing blood-brain barrier damage, thus playing a role in brain protection. Our study may provide a novel therapeutic strategy for SAE.
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NUTRITIONAL NEUROSCIENCE
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2022
·
IF 4.062
Yumin Liu,Ziqi Hu,Jing Wang,Yanjun Liao,Luan Shu
Objective Depression is one of the most common complications in patients with diabetes. Our previous study demonstrated puerarin, a dietary isoflavone, improved glucose homeostasis and β-cell regeneration in high-fat diet (HFD)-induced diabetic mice. Here, we aim to evaluate the potential effect of puerarin on diabetes-induced depression.Methods The co-occurrence of diabetes and depression with related biochemical alterations were confirmed in HFD mice and db/db mice, respectively using behavioral analysis, ELISA and western blotting assay. Furthermore, impacts of puerarin on depression-related symptoms and pathological changes were investigated in HFD mice.Results The results showed that puerarin effectively alleviated the depression-like behaviors of HFD mice, down-regulated serum levels of corticosterone and IL-1β, while up-regulated the content of 5-hydroxytryptamine. Simultaneously, puerarin increased the number of hippocampal neurons in HFD mice, and suppressed the apoptosis of neurons to protect the hippocampal neuroplasticity. GLP-1R expression in hippocampus of HFD mice was enhanced by puerarin, which subsequently activated AMPK, CREB and BDNF/TrkB signaling to improve neuroplasticity. Importantly, our data indicated that puerarin had an advantage over fluoxetine or metformin in treating diabetes-induced depression.Conclusion Taken together, puerarin exerts anti-depressant-like effects on HFD diabetic mice, specifically by improving hippocampal neuroplasticity via GLP-1R/BDNF/TrkB signaling. Puerarin as a dietary supplement might be a potential candidate in intervention of diabetes with comorbid depression.
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Food Bioscience
·
2026
·
IF 6.2
Shirui Zhang,Zihao Tian,Jingcan Wen,Xiaohui Ren,Bing Zhou,Mingjin Song,Wei Yu,Jiage Ma
Combined L. plantarum and PUE attenuated HFrD-induced HUA and cognitive impairment. • Combined intervention lowered serum UA, hepatic XOD, and renal urate transporters. • Combined intervention increased PSD95/BDNF and ERK/CREB phosphorylation in brain. • The combination attenuated cecal microbiota alterations and colonic injury. Hyperuricemia (HUA) is a common metabolic disorder, which poses a threat to human health. This study examined the impact of Lactiplantibacillus plantarum KLDS 1.0328 (LP) in combination with puerarin (PUE) on high-fructose diet (HFrD)-induced HUA and associated cognitive impairment. Following the combined intervention, the levels of serum creatinine (CRE), blood urea nitrogen (BUN) and uric acid (UA) were decreased, while pathological damage, inflammatory responses, and oxidative stress in the kidney were alleviated. Moreover, the combined intervention reduced hepatic xanthine oxidase (XOD) activity and renal glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) protein expression. Notably, cognitive function was ameliorated in behavioral assessments. Furthermore, this intervention attenuated neuroinflammation and oxidative stress in brain tissues, while Synapsin I and postsynaptic density protein 95 (PSD95) expression, extracellular signal-regulated kinase (ERK) and cAMP response element-binding protein (CREB) phosphorylation, and brain-derived neurotrophic factor (BDNF) expression were increased in the LP + PUE-4 group. Additionally, the HFrD-induced gut microbiota dysbiosis was partially alleviated, particularly by shifting the Firmicutes/Bacteroidota ratio and modulating specific bacterial genera. Collectively, these findings indicated that the combined administration of L. plantarum KLDS 1.0328 and PUE attenuated HFrD-induced HUA and associated cognitive impairment when initiated concurrently with HFrD feeding. Download: Download high-res image (339KB) Download: Download full-size image
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Small
·
2024
·
IF 13
Liguo Jin,Hanpeng Liu,Chaofeng Wang,Xiangmei Liu,Congyang Mao,Yu Zhang,Zhaoyang Li,Shengli Zhu,Hui Jiang,Zhenduo Cui,Yufeng Zheng,Shuilin Wu
Staphylococcus aureus ( S. aureus )-infected osteomyelitis is a deep tissue infection that cannot be effectively treated with antibiotics. Microwave (MW) thermal therapy (MTT) and MW dynamic therapy (MDT) based on MW-responsive materials are promising for the therapy of bacteria-infected osteomyelitis occurring in deep tissues that cannot be effectively treated with antibiotics. In this work, the MW-responsive system of carbon nanotubes (CNTs)/Prussian blue (PB)/puerarin (Pue) with stable network-like structures is constructed. The PB is grown in situ on the CNTs, and its introduction not only reduces the aggregation of the network-like structures of the CNTs, but the large specific surface area and mesoporous structure can also provide many active sites for the adsorption of oxygen and polar molecules. Pue is a natural anti-inflammatory material that reduces inflammation at the infection site. The composite of the CNTs and PB avoids the skin effect and thus can improve dielectric and reflection losses. The MW thermal response of CNTs/PB/Pue is mainly due to the occurrence of reflection loss, dielectric loss, multiple reflections and scattering, interface polarization, and dipole polarization. In addition, under MW irradiation, the CNTs/PB/Pue can produce reactive oxygen species (ROS), such as singlet oxygen ( 1 O 2 ), hydroxyl radical (·OH).
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Nutrients
·
2024
·Vol. 16
·期 9
·1273
·
IF 4.8
Yu Mu,Yalin Yang,Shuang Jiang,Chilu Liu,Yanxing Han,Jiandong Jiang,Yuhong Wang
Metabolic syndrome (MetS) is a cluster of risk factors for cardiovascular diseases (CVDs) that has become a global public health problem. Puerarin (PUE), the principal active compound of Pueraria lobata, has the effects of regulating glucose and lipid metabolism and protecting against cardiovascular damage. This study aimed to investigate whether dietary supplementation with PUE could ameliorate MetS and its associated cardiovascular damage. Rats were randomly divided into three groups: the normal diet group (NC), the high-fat/high-sucrose diet group (HFHS), and the HFHS plus PUE diet group (HFHS-PUE). The results showed that PUE-supplemented rats exhibited enhanced glucose tolerance, improved lipid parameters, and reduced blood pressure compared to those on the HFHS diet alone. Additionally, PUE reversed the HFHS-induced elevations in the atherogenic index (AI) and the activities of serum lactate dehydrogenase (LDH) and creatine kinase (CK). Ultrasonic evaluations indicated that PUE significantly ameliorated cardiac dysfunction and arterial stiffness. Histopathological assessments further confirmed that PUE significantly mitigated cardiac remodeling, arterial remodeling, and neuronal damage in the brain. Moreover, PUE lowered systemic inflammatory indices including C-reactive protein (CRP), neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), and systemic immune-inflammation index (SII). In conclusion, dietary supplementation with PUE effectively moderated metabolic disorders, attenuated systemic inflammation, and minimized cardiovascular damage in rats with MetS induced by an HFHS diet. These results provide novel insights into the potential benefits of dietary PUE supplementation for the prevention and management of MetS and its related CVDs.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chenran Xin,Pinhui Jia,Yan Zhao,Zhiqiang Cheng,Wencong Liu,Peng Di,Wei Li,Hongyan Zhu
The healing of wounds in aging skin is a challenging issue that has not been thoroughly studied. Composite hydrogels made from natural polysaccharides have shown potential as dressings for various types of wounds. In this study, we prepared a polysaccharide-based composite hydrogel to provide a new strategy for treating aging skin wounds. First, chitosan (CS) was modified and Gastrodin (GAS) was grafted onto its main chain structure to obtain GAS/CS. Then GAS/CS was mixed with oxidized Gastrodia elata polysaccharides (GEP) to form a hydrogel using the principle of adsorbent reaction, and Puerarin (PUE), a natural plant ingredient, was embedded by gelatin microspheres and then loaded into the hydrogel. As a result, the composite hydrogel effectively reduced oxidative stress in tissue cells at the wound site and inhibited bacterial growth. It also reduced inflammation, promoted angiogenesis, and enhanced collagen deposition, which facilitated the repair of all aspects of the wound healing process in aging mouse skin. In short, this study explored the anti-aging effects of polysaccharides from Gastrodia elata , which might serve as both a treatment and an auxiliary for aging skin wounds. Additionally, the Gastrodia elata polysaccharide hydrogel was expected to be a promising drug carrier for skin repair.
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Journal of Orthopaedic Translation
·
2022
·
IF 4.889
Huijuan Cao,Lingli Li,Ling Li,Xiangbo Meng,Yanzhi Liu,Wenxiang Cheng,Peng Zhang,Yongbo Gao,Ling Qin,Xinluan Wang
Objectives Large bone defect repair is a challenging clinical problem due to limited self-repair ability. A well-designed bone filling product should possess the ability to induce tissue in-growth and facilitate neovascularization and new bone formation. Puerarin has been used in clinics for a long time, and recently it was found to be able to promote osteogenesis. This study aimed to investigate a puerarin-based drug/delivery combination implant for promoting large bone defect repair. Methods Puerarin was incorporated into the poly (lactic-co-glycolic acid)/β-calcium phosphate (PLGA/TCP, PT) to form a porous PLGA/TCP/Puerarin (PTP) composite scaffold by low-temperature rapid prototyping technology. Its structural and degradation were analyzed in vitro . Then we employed a rat calvarial critical size defect model to assess the potency of the PTP scaffold. MC3T3-E1 cells and EA. hy 926 cells were used to investigate the underlying mechanism. Results PTP scaffold inherited all advantages of PT scaffold in structural, mechanical, and biodegradation , meanwhile puerarin stably and continuously released from PTP scaffold and lasted for 5 months in vitro . At 8 weeks after implantation, the PTP scaffold triggered new bone formation in the macro-pores of the scaffold and inside the scaffold accompanied by the degrading materials. The underlying mechanism revealed that the PTP scaffold induced vascular infiltration and recruit repair cells through stimulating vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2) expressions to promote angiogenesis and osteogenesis. Conclusion Puerarin-enriched porous PTP scaffold was a promising local delivery system with sustained release of puerarin for facilitating defect repair through getting synergistic angiogenic and osteogenic effects. The Translational Potential of this Article The PTP scaffold presents a potential drug/device combination medical implant for large bone defect repair, which also provides a new and innovative application for the “old drug” puerarin.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Wei Liu,Zhaoliang Wu,Yanji Wang,Huijie Zheng,Hao Yin
At present, the large scale application of batch foam fractionation is still in confrontation with many problems and difficulties, especially for the enrichment of non-surface-active materials. It is difficult to select a suitable surfactant as the collector when a non-surface-active material can only form a stable association with the expensive surfactants or has not any interactions with the commercially available surfactants. The objective of this work was to investigate the feasibility of batch foam fractionation for recovering such a non-surface-active material, puerarin. Yam mucilage, an abundant byproduct from the manufacture of yam starch, was used to generate a stable foam phase based on its good foamability. According to the properties of puerarin and yam mucilage, β -cyclodextrin ( β -CD) was selected as a basic material to establish an association between them. The results of fluorescence sensitization experiments indicated that puerarin could form an equimolar inclusion complex with β -CD and the inclusion constant was 405.8. Subsequently, β -CD was modified by introducing carbon disulfide and copper (Cu) ion. Then, puerarin could be adsorbed on the gas-liquid interface in the form of ternary complex with modified β -CD-Cu ion complex and glycoprotein molecules (see Fig. 7). Batch foam fractionation was carried out by using the crude extracting solution as the initial feeding solution, in which the concentration of puerarin was as high as 4.6 g/L. Under the suitable conditions of temperature 50 °C, pH 6.0, loading volume of feed solution 400 mL, the pore diameter of gas distributor 0.450 mm, additive volume of yam mucilage 30 mL and volumetric air flow rate 100 mL/min, the enrichment ratio and the percent recovery of puerarin were 4.3 and 82.3%, respectively. This strategy could be generalized to the separation of various non-surface-active materials due to the low cost and the diversiform cavity dimension of cyclodextrins.
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 4
·1230
·
IF 4.927
Li Jiang,Yanling Xiong,Yu Tu,Wentong Zhang,Qiyun Zhang,Peng Nie,Xiaojun Yan,Hongning Liu,Ronghua Liu,Guoliang Xu
Puerarin (PUR) and gastrodin (GAS) are often used in combined way for treating diseases caused by microcirculation disorders. The current study aimed to investigate the absorption and transportation mechanism of PUR and GAS and their interaction via Caco-2 monolayer cell model. In this work, the concentration in Caco-2 cell of PUR and GAS was determined by HPLC method. The bidirectional transport of PUR and GAS and the inhibition of drug efflux including verapamil and cyclosporine on the transport of these two components were studied. The mutual influence between PUR and GAS, especially the effect of the latter on the former of the bidirectional transport were also investigated. The transport of 50 μg·mL−1PUR in Caco-2 cells has no obvious directionality. While the transport of 100 and 200 μg·mL−1PUR presents a strong directionality, and this directionality can be inhibited by verapamil and cyclosporine. When PUR and GAS were used in combination, GAS could increase the absorption of PUR while PUR had no obvious influence on GAS. Therefore, the compatibility of PUR and GAS is reasonable, and GAS can promote the transmembrane transport of PUR, the effect of which is similar to that of verapamil.Keywords:transport mechanism;puerarin;gastrodin;absorption;Caco-2 cell monolayer
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危险品化学品经营许可证（带存储）