MOLECULES
·
2017
·Vol. 22
·期 11
·1814
·
IF 2.861
Wenrui Wang,Lingyu Zhang,Tiantian Chen,Wen Guo,Xunxia Bao,Dandan Wang,Baihui Ren,Haifeng Wang,Yu Li,Yueyue Wang,Sulian Chen,Baoding Tang,Qingling Yang,Changjie Chen
In this study, resveratrol-loaded solid lipid nanoparticles (Res-SLNs) were successfully designed to treat MDA-MB-231 cells. The Res-SLNs were prepared using emulsification and low-temperature solidification method. The Res-SLNs were spherical, with small size, negative charge, and narrow size distribution. Compared with free resveratrol, the Res-SLNs displayed a superior ability in inhibiting the proliferation of MDA-MB-231 cells. In addition, Res-SLNs exhibited much stronger inhibitory effects on the invasion and migration of MDA-MB-231 cells. Western blot analysis revealed that Res-SLNs could promote the ratio of Bax/Bcl-2 but decreased the expression of cyclinD1 and c-Myc. These results indicate that the Res-SLN may have great potential for breast cancer treatment.Keywords:solid lipid nanoparticle;resveratrol;breast cancer;cyclinD1
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Dongliang Zhang,Yanfei Cao,Chengye Ma,Shanfeng Chen,Hongjun Li
There is a paradox when incorporating enzyme into an edible chitosan film that chitosan is dissolved in acid solution and enzyme activity is maintained under mild conditions. A method for maintaining the pH of the chitosan solution at 4–6 to prepare a chitosan film containing β-cyclodextrin, resveratrol−β-cyclodextrin inclusion (RCI), was developed, using glucamylase and acetic acid. A considerable amount of resveratrol was released by the glucamylase-incorporated film within 15 days, and the maximum amount released was 46% of the total resveratrol content. The highest resveratrol release ratio (released resveratrol/total resveratrol) was obtained in the film with 6 mL of RCI. Scratches and spores were generated on the surface of the glucamylase-added film immersed in water (GAFW) for 7 days because of β-cyclodextrin hydrolysis during film drying and water immersion. RCI and β-cyclodextrin were extruded from the film surface and formed teardrops, which were erased by water on the GAFW surface but appeared on the glucamylase-added film without water immersion (GAF). The bubbles generated by the reaction of acetic acid and residual sodium bicarbonate were observed in both glucamylase-free films immersed in water (GFFW) for 7 days and without water immersion (GFF). The FT-IR spectra illustrated that the covalent bond was not generated during water immersion and β-cyclodextrin hydrolysis. The crystal structure of chitosan was destroyed by water immersion and β-cyclodextrin hydrolysis, resulting in the lowest chitosan crystallization peak at 22°. The increasing of water holding capacity determined by EDX presented the following order: GAF, GFFW, GFF, and GAFW.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 8
·1246
·
IF 4.7
Wen Zhang,Lingli Meng,Xinyi Lv,Limin Wang,Pei Zhao,Jinrong Wang,Xinping Zhang,Jinyu Chen,Zijian Wu
A reliable strategy for improving the stability and shelf life of protein-stabilized systems is by covalently attaching the protein onto a polysaccharide. In this study, ovalbumin (OVA) was modified with dextran (DEX) of different molecular weights by the Maillard reaction, and was used to enhance the stability of emulsions loaded with resveratrol. The surface hydrophobicity, thermal stability, and FT-IR spectroscopy of the OVA–DEX conjugates were evaluated. The results showed that the surface hydrophobicity of OVA decreased, while the thermal stability of OVA was significantly improved after DEX covalent modification. The OVA–DEX1k-stabilized emulsion exhibited high encapsulation efficiency of resveratrol, with the value of 89.0%. In addition, OVA–DEX was considerably more effective in droplet stabilization against different environmental stresses (heat, pH, and ionic strength). After 28 days of storage at 25 °C, the OVA-stabilized emulsion showed faster decomposition of resveratrol, whereas the OVA–DEX-conjugate-stabilized emulsion had approximately 73% retention of resveratrol. Moreover, the antioxidant activity of resveratrol-loaded emulsions stabilized by OVA–DEX was higher during storage under different temperatures. These results proved that the OVA–DEX conjugates had the potential to form stable, food-grade emulsion-based delivery systems against environmental stresses, which strongly supports their potential in the field of food and biomedical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2024
·
IF 3.8
Jingwen Xu,Guangwen Luo,Yue Zhang,Kaiqiang Lv,Sijuan Wang,Zuheng Shan,Xiang Gao,Guoliang Li
To improve the limited bioavailability of resveratrol (RSV) due to its poor water solubility, stability and bioaccessibility, there is an urgent need for the development of food-grade delivery systems. Herein, RSV was loaded into Spirulina platensis ( SP ) through passive diffusion, electrostatic and hydrogen bond interactions to create a Spirulina platensis ( SP )-based edible vehicle called SP @RSV, with a maximum loading content of 45.36 ± 1.08 % and loading efficiency of 86.72 ± 4.41 %. RSV was endowed with ultraviolet resistance after loading. SP @RSV showed both a broad-spectrum scavenging capacity against free radicals after digestion of gastrointestinal fluids, and a high bioaccessibility. Furthermore, SP @RSV could significantly prolong the life span of Caenorhabditis elegans by enhancing the activity of antioxidases and without causing acute toxicity. Besides, SP @RSV-contained jelly was prepared and sensory evaluated to showcase its potential utilization in the nutraceuticals industry. These findings confirm the feasibility of using SP as a vehicle to enhance the stability, intestinal targeting and bioaccessibility of RSV.
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2024
·
IF 4.1
Xiang Tao,Mengjie Wei,Xianfei Hu,Yong Tang,Wei Wei,Jingcheng Liu,Xiaojie Li
Hyperbranched epoxy resins are efficient modifiers for epoxy resins but still require simultaneous improvements in terms of thermomechanical/mechanical properties and thermal stability. Herein, a novel resveratrol-based hyperbranched polyether epoxy resin (HBPEER) was designed for all-purpose epoxy resin modification. To this end, hyperbranched polyether with terminal phenolic hydroxyl groups was first synthesized from commercially available 1,6-dibromohexane (A 2 -type monomer) and resveratrol (B'B 2 -type monomer). Surface modification with epichlorohydrin was afterward implemented to produce hyperbranched polyether with terminal epoxy groups (HBPEER). Next, various HBPEER-modified epoxy thermosets were prepared and characterized. The results suggested that compared to the neat diglycidyl ether of bisphenol A (DGEBA) thermoset, the 6 wt% HBPEER-modified epoxy thermoset showed an increase in impact strength, elongation at break, critical stress intensity factor (K IC ), critical crack propagation energy release rate (G IC ), flexural strength, tensile strength, storage modulus, glass transition temperature (T g ), and initial thermal decomposition temperature (T d5% ) by 69.6 %, 87.0 %, 32.0 %, 68.2 %, 29.3 %, 37.0 %, 8.1 %, 3.5 %, and 1.6 % respectively. Moreover, the dielectric constant and loss of 6 wt% HBPEER-modified epoxy thermoset decreased by 16.2 % and 16.5 %, respectively. Such comprehensive performance modifications were caused by the combined effects of crosslink density improvement, efficient intramolecular cavity, rigid stilbene units, and flexible aliphatic backbone of HBPEER.
✓ 已验证
产品关联
DOI
高影响因子
Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology
·
2018
·Vol. 301
·期 7
·1179-1188
·
IF 1.373
Wei Cao,Junqiang Zhang,Gaoyuan Wang,Jinsen Lu,Taorong Wang,Xiaoyu Chen
In rheumatoid arthritis patients, the fibroblast-like synovial cells (FLS) growth is not controlled normally, but is similar to the tumor cells proliferation in histology. Our previous studies have shown that resveratrol inhibits the proliferation of FLS and promotes FLS apoptosis. However, the molecular mechanisms involved in resveratrol-induced FLS apoptosis have not been determined yet. Here, we showed that the FLS cell viability (following pretreatment with 5 µM H 2 O 2 for 24 hr) exhibited better proliferation performance than at other concentrations via the CCK-8 assay. The cell apoptotic rate increased with the increasing concentration of resveratrol (0, 40, 80, 160, 320 μM), as detected by TdT-mediated dUTP nick-end labeling (TUNEL) staining and western blotting. Furthermore, the expression level of autophagy-related proteins (LC3A/B, ATG-5) decreased with the increased concentration of resveratrol, as determined by immunofluorescence and western blot analysis. We also showed that resveratrol induced FLS mitochondrial morphology change. Moreover, mitochondrial function detection showed that the mitochondrial membrane potential was lost with the increased concentration of resveratrol as examined by the JC-1 assay. The production of ATP in cells was positively and negatively correlated with the resveratrol concentration. Simultaneously, the intracellular calcium release and calcium influx decreased gradually with the increase in resveratrol concentration. Therefore, we proposed that resveratrol can reduce the level of autophagy in FLS. The decrease in the autophagy level can lead to the accumulation of reactive oxygen species, which may result in mitochondrial dysfunction and promotion of FLS apoptosis. Anat Rec, 301:1179–1188, 2018. © 2018 Wiley Periodicals, Inc.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 13
·2078
·
IF 4.7
Jianan Liu,Qian Zhang,Hongshun Hao,Jingran Bi,Hongman Hou,Gongliang Zhang
The aim of our study was to investigate whether the combination of benzyl isothiocyanate (BITC) and resveratrol (RES) has a synergistic effect on the inhibition of inflammation in colitis. The results revealed that the BITC and RES combination (BITC_RES) was more effective than either substance alone at significantly alleviating the symptoms of dextran sodium sulfate (DSS)-induced colitis in mice, including the prevention of colon shortening and loss of body weight, a reduction in the disease activity index, and prevention of colon damage. Similarly, compared with the DSS group, BITC_RES reduced myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) levels in the mouse colon by 1.4–3.0-fold and 1.4-fold, respectively. In addition, the combination of BITC and RES upregulated the inflammatory factor IL-10 by 1.3- and 107.4-fold, respectively, compared to the individual BITC and RES groups, whereas the proinflammatory factors, including TNF-α, IL-1β, and IL-6, were downregulated by 1.1–7.4-, 0.7–3.6-, and 0.6–2.6-fold, respectively, in the BITC_RES group compared with the individual groups. Gut microbiome analysis indicated that BITC_RES remodeled the structure of gut bacteria at the phylum, family, and genus levels, upregulating the abundance of the phylum Bacteroidetes and the familyMuribaculaceaeand the genus norank_f_Muribaculaceaeand downregulating the abundance of the phylum Firmicutes. Significant correlations between the relative levels of these proinflammatory cytokines and changes in the gut microbiota were found using Pearson’s correlation analysis. BITC and RES exhibited synergistic effects by reshaping the gut microbiota and modulating the level of serum cellular inflammatory factors, thus exerting a protective effect against colitis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Fengjiao Zhang,Jinguang Liu,Victoria Anthony Uyanga,Caiyun Tang,Yanan Qu,Xu Qin,Yilun Chen,Yuqian Liu
BACKGROUND As the major protein (approximately 36%) in rice bran, globulin exhibits excellent foaming and emulsifying properties, endowing its useful application as a foaming and emulsifying agent in the food industry. However, the low water solubility restricts its commercial potential in industrial applications. The present study aimed to improve this protein's processing and functional properties. RESULTS A novel covalent complex was fabricated by a combination of the Maillard reaction and alkaline oxidation using rice bran globulin (RBG), chitooligosaccharide (C), quercetin (Que) and resveratrol (Res). The Maillard reaction improved the solubility, emulsifying and foaming properties of RBG. The resultant glycosylated protein was covalently bonded with quercetin and resveratrol to form a (RBG-C)-Que-Res complex. (RBG-C)-Que-Res exhibited higher thermal stability and antioxidant ability than the native protein, binary globulin-chitooligosaccharide or ternary globulin-chitooligosaccharide-polyphenol (only containing quercetin or resveratrol) conjugates. (RBG-C)-Que-Res exerted better cytoprotection against the generation of malondialdehyde and reactive oxygen species in HepG2 cells, which was associated with increased activities of antioxidative enzymes superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) through upregulated genes SOD1, CAT, GPX1 (i.e. gene for glutathione peroxidase-1), GCLM (i.e. gene for glutamate cysteine ligase modifier subunit), SLC1A11 (i.e. gene for solute carrier family 7, member 11) and SRXN1 (i.e. gene for sulfiredoxin-1). The anti-apoptotic effect of (RBG-C)-Que-Res was confirmed by the downregulation of caspase-3 and p53 and the upregulation of B-cell lymphoma-2 gene expression. CONCLUSION The present study highlights the potential of (RBG-C)-Que-Res conjugates as functional ingredients in healthy foods. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS LETTERS
·
2024
·
IF 2.7
Shide Li,Hongsheng Luo,Ronghua Qiu,Kaibin Huang,Ziying Chen,Xiangjun Fu
In order to improve the aqueous solubility and enhance stability of resveratrol (RES), an inclusion complex (RES-ECH-βCD) of RES with epichlorohydrin-modified β-cyclodextrin (ECH-βCD) was prepared using freeze-drying. The effects of RES-ECH-βCD on the solubility and stability of RES were investigated. Phase solubility studies indicated that ECH-βCD significantly enhanced the solubility of RES compared to β-cyclodextrin (β-CD). Thermal stability tests showed that the inclusion complex exhibited superior thermal stability compared to free RES. Furthermore, the application of RES-ECH-βCD in sunscreen formulations was validated. The formulation exhibited sun protection properties, with RES-ECH-βCD working synergistically with α-glucosyl rutin (Rutin-G) to significantly enhance both SPF and PFA. These findings suggest that RES-ECH-βCD not only improves the solubility and stability of RES but also has promising potential for use in cosmetic and sunscreen products.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2025
·
IF 9
Qian Tan,Wentian Zhang,Cunxian Lai,Xinyu Zhi,Lichao Xia,Fangang Meng,Shanshan Zhao
Nanofiltration (NF) is a pivotal technology in advancing water purification, achieving a harmonious balance between high selectivity and energy efficiency. However, current thin-film composite (TFC) NF membranes often encounter challenges in simultaneously achieving high permeability and selectivity, while exhibiting robust antifouling performance. Herein, we introduce an innovative fabrication approach that incorporates resveratrol (Res) to form a functional interlayer to regulate interfacial polymerization (IP) process for developing a polyamide (PA) NF membrane with enhanced permeability and antifouling property. Ascribing to the hydrogen bonding and electrostatic interactions between the Res and piperazine (PIP), the aqueous phase diffusion rate of PIP molecules was effectively moderated, thus resulting in a finely controlled formation of an ultrathin (31.3 nm) and low cross-linked PA selective layer. The optimized Res-regulated TFC membrane exhibited a water permeance of 20.0 ± 1.0 L m -2 h -1 bar -1 , which is more than four times higher than that of control membrane. In addition, it presented exceptional ion selectivity for NaCl/ Na 2 SO 4 , with a value of 72.2, which is superior to the performance of most state-of-the-art PA NF membranes. Furthermore, it exhibited excellent antifouling properties during simulated and actual wastewater treatment. This environmentally friendly and cost-effective IP-regulation strategy shows great potential for design and engineering high-performance NF membranes for a wide range of water treatment applications.
✓ 已验证
产品关联
DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2025
·
IF 3.4
Ling Chen,Xiao-Qin Ma,Hai-Li Wu,Shun Zhang,Jie-Feng Chen,Ning Lin,Qing Chen
Glioblastoma is a highly aggressive brain tumor with a poor prognosis and limited treatment options. Resveratrol (RES) and temozolomide (TMZ) both showed significant antitumor activity in GBM therapy. However, they are limited by poor solubility and stability, respectively. This study reports the first successful development of a resveratrol–temozolomide (RES–TMZ) cocrystal. The cocrystal significantly improved the solubility of RES and stability of TMZ, while it exhibited enhanced oral bioavailability in rats. In vitro cytotoxicity assays revealed that the RES–TMZ cocrystal showed superior inhibitory effects on U87 glioma cell proliferation and induced a higher apoptosis rate compared to that of monotherapy or physical mixtures. Mechanistic studies further demonstrated that the cocrystal exerts its effects through the mitochondrial-dependent apoptotic pathway, characterized by the upregulation of the proapoptotic protein Bax, downregulation of the antiapoptotic protein Bcl-2, activation of the caspase-3 pathway, and collapse of mitochondrial membrane potential coupled with a burst of intracellular reactive oxygen species. This work establishes pharmaceutical cocrystallization as a viable strategy to develop improved combination therapies for glioblastoma by overcoming drug limitations while potentiating therapeutic effects.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Ping Shao,Pei Wang,Ben Niu,Ji Kang
Based on the hydrogen bonding ability of polysaccharides and phospholipids and steric hindrance, nanoliposomes (LPs), pectin-coated resveratrol liposomes (LMP-LPs and HMP-LPs) with different esterification degrees (DE = 30%, 70%) were prepared. The entrapment efficiency was 78 ± 5%. The addition of pectin with different degree of esterification (DE = 0%, 30%, 70%) increased the particle size of liposomes from 102 nm to 122 nm and 140 nm and negative charge change from −13.63 mV to −20.96 mV and −30.11 mV respectively. This is mainly due to the adsorption of pectin on the liposome surface. Some environmental factors such as pH, ionic strength and temperature have a significant effect on the appearance, particle size and leakage rate of liposomes. Experiments have shown that the leakage rate of low-methoxy pectin-coated liposomes resveratrol is lower, and this work provides useful information on the use of low-methoxy pectin for controlling food release.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）