MOLECULAR IMMUNOLOGY
·
2018
·
IF 3.188
Gang Chi,Xin-Xia Feng,Ying-Xia Ru,Ting Xiong,Yuan Gao,Han Wang,Zhen-Long Luo,Ran Mo,Fang Guo,Yong-Pei He,Gui-Mei Zhang,De-An Tian,Zuo-Hua Feng
Autoimmune hepatitis (AIH), a serious autoimmune liver disease, can be a lifelong illness, leading to fibrosis, cirrhosis, and hepatocellular carcinoma (HCC). So far the mechanisms for disease initiation are largely unknown. Here we report that the amplified non-AIH liver inflammation could promote the initiation of AIH due to the sustained increase of IL-6, IL-12, IL-4, and IL-25 in the liver. The liver injury resulting from virus (adenovirus) or chemicals (CCl 4 ) could induce an amplified (stronger/long-lasting) hepatic inflammation by releasing the ligands for TLR2/TLR4. The amplified inflammation resulted in the increase of multiple cytokines and chemokines in the liver. Among them, the sustained increase of IL-6/IL-12 resulted in the activation of STAT3 and STAT4 in hepatic CD4 + CD25 + Treg cells, thus suppressing Foxp3 gene expression to reduce the suppressive function of Treg cells in the liver, but not those in the spleen. The increase of IL-12 and the impairment of Treg function promoted Th1 response in presence of self-mimicking antigen (human CYP2D6). Intriguingly, the amplified inflammation resulted in the increase of IL-4 and IL-25 in the liver. The moderate increase of IL-4 was sufficient for cooperating with IL-25 to initiate Th2 response, but inefficient in suppressing Th1 response, favoring the initiation of autoimmune response. Consequently, either adenovirus/CYP2D6 or CCl 4 /CYP2D6 could induce the autoimmune response and AIH in the mice, leading to hepatic fibrosis. The findings in this study suggest that the amplified non-AIH inflammation in the liver could be a driving force for the initiation of autoimmune response and AIH.
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高影响因子
CRYSTENGCOMM
·
2018
·Vol. 20
·期 31
·4383-4394
·
IF 3.304
Shuangyu Xin,Miao Gao,Chuang Wang,Xuejiao Wang,Ge Zhu,Faguang Zhou,Zhuowei Li,Yuhua Wang
To explore a new family of phosphate phosphors for application in ultraviolet-based light emitting diodes (LEDs), novel promising blue emission solid solution phosphors, Ca1−xSrxHf4(PO4)6:Eu2+, with efficient and controllable photoluminescence were synthesized and characterized in the full range of 0 ≤ x ≤ 1. To deeply understand the relationship between the structure and properties of the phosphors, the phase purities, crystal structures and linear structural evolutions of Ca1−xSrxHf4(PO4)6:Eu2+ solid solutions were investigated in detail by FT-IR spectroscopy and Rietveld structure refinement of their powder XRD patterns and verified by high transmission electron microscopy (HRTEM). The linear structural evolutions induced controllable blue emission with high quantum efficiency and excellent thermal stability in the Ca1−xSrxHf4(PO4)6:Eu2+ solid-solution phosphors. Moreover, the photoluminescence properties in Ca1−xSrxHf4(PO4)6:Eu2+ can be optimized by adjusting the components. The mechanism of the controllable luminescence properties was also investigated in detail. Excellent thermal stability was obtained in Ca1−xSrxHf4(PO4)6:Eu2+, and the thermal quenching mechanism was investigated. Finally, white LEDs were fabricated and their electroluminescence properties were investigated. This simple and effective method to optimize the photoluminescence properties of phosphors can be used for designing other novel solid-solution phosphors.
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RSC Advances
·
2018
·Vol. 8
·期 72
·41368-41375
·
IF 2.936
Liangliang He,Jinjin Xu,Qi Wang,Yezi Zhang,Zifei Qin,Yang Yu,Zhengming Qian,Zhihong Yao,Xinsheng Yao
Shogaols, mainly [6]-shogaol (6S), [8]-shogaol (8S) and [10]-shogaol (10S), the predominant and characteristic pungent phytochemicals in ginger, are responsible for most of its beneficial effects. However, poor oral bioavailability owing to extensive glucuronidation limits their application. The present study aimed to characterize the glucuronidation pathways of 6S, 8S and 10S by using pooled human liver microsomes (HLM), human intestine microsomes (HIM) and recombinant human UDP-glucosyltransferases (UGTs). The rates of glucuronidation were determined by incubating shogaols with uridine diphosphate glucuronic acid-supplemented microsomes. Kinetic parameters were derived by appropriate model fitting. Reaction phenotyping assays, activity correlation analyses and relative activity factors were performed to identify the main UGT isoforms. As a result, one mono-4′-O-glucuronide was detected after incubating each shogaol with HLM and HIM. Enzymes kinetic analysis demonstrated that glucuronidation of shogaols consistently displayed the substrate inhibition profile, and the liver showed higher metabolic activity for shogaols (CLint = 1.37–2.87 mL min−1 mg−1) than the intestine (CLint = 0.67–0.85 mL min−1 mg−1). Besides, reaction phenotyping assays revealed that UGT2B7 displayed the highest catalytic ability (CLint = 0.47–1.17 mL min−1 mg−1) among all tested UGTs. In addition, glucuronidation of shogaols was strongly correlated with AZT glucuronidation (r = 0.886, 0.803 and 0.871 for glucuronidation of 6S, 8S and 10S, respectively; p < 0.01) in a bank of individual HLMs (n = 9). Furthermore, UGT2B7 contributed to 40.8%, 34.2% and 36.0% for the glucuronidation of 6S, 8S and 10S in HLM, respectively. Taken altogether, shogaols were efficiently metabolized through the glucuronidation pathway, and UGT2B7 was the main contributor to their glucuronidation.
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Food Analytical Methods
·
2014
·Vol. 8
·期 2
·428-437
·
IF 1.802
Zeng Fang,Ou Jun,Huang Yufen,Li Qian,Xu Guizhi,Liu Zhongzhen,Yang Shaohai
The present paper reports the application of an optimized pre-column derivatization procedure with aminoquinolyl- N -hydroxysuccinimidyl carbamate (AQC) to the determination of 21 free amino acids in fruit juices. The method permitted the optimal separation of 21 amino acids and ammonium in 30 min. Excellent response linearity was obtained from 2 to 1,000 μM for all amino acids, except tryptophan, for which the linearity was 5–1,000 μM. The fluorescence detection limits ranged from 0.08 pmol (phenylalanine) to 0.69 pmol (cysteine), except for tryptophan (7.89 pmol), for a signal-to-noise ratio of 3. The relative standard deviations of peak areas ranged from 0.1 to 0.4 % for intraday analysis and from 2.1 to 5.0 % for interday analysis. The method was applied to analyze the free amino acids in six fruit juices, and the recoveries of the proposed method were 90.4 to 103.3 %. The total free amino acid content ranged from 56.97 mg L −1 (apple) to 469.45 mg L −1 (longan) in six fruit juices. Aspartic acid (Asp), glutamic acid (Glu), asparagine (Asn), serine (Ser), and glutamine (Gln) were present in higher levels in most samples, whereas cysteine (Cys) was below the detection limit in the selected samples. Notable advantages of this method include its speed, ease, low cost, good repeatability, high sensitivity, excellent precision and accuracy, and anti-interference in high-sugar matrices.
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BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Muhammad Waheed Iqbal,Xinrui Tang,Tahreem Riaz,Shahid Mahmood,Yufei Zhang,Mei Zhao,Junhua Yun,Jia Li,Xianghui Qi
6-Deoxy- l -sorbose (6-DLS) is an imperative rare sugar employed in food, agriculture, pharmaceutical and cosmetic industeries. However, it is a synthetic and very expensive rare sugars, previously synthesized by chemo-enzymatic methods through a long chain of chemical processes. Recently, enzymatic synthesis of rare sugars has attracted a lot of attention due to its advantages over synthetic methods. In this work, a promising approach for the synthesis of 6-DLS from an inexpensive sugar l -fucose was identified. The genes for l -fucose isomerase from Paenibacillus rhizosphaerae (Pr-LFI) and genes for d -tagatose-3-epimerase from Caballeronia fortuita (Cf-DTE) have been used for cloning and co-expression in Escherichia coli , developed a recombinant plasmid harboring pANY1-Pr-LFI/Cf-DTE vector. The recombinant co-expression system exhibited an optimum activity at 50 °C of temperature and pH 6.5 in the presence of Co 2+ metal ion which inflated the catalytic activity by 6.8 folds as compared to control group with no metal ions. The recombinant co-expressed system was stable up to more than 50 % relative activity after 12 h and revealed a melting temperature (T m ) of 63.38 °C exhibiting half-life of 13.17 h at 50 °C. The co-expression system exhibited, 4.93, 11.41 and 16.21 g/L of 6-DLS production from initial l -fucose concentration of 30, 70 and 100 g/L, which equates to conversion yield of 16.44 %, 16.30 % and 16.21 % respectively. Generally, this study offers a promising strategy for the biological production of 6-DLS from an inexpensive substrate l -fucose in slightly acidic conditions with the aid of co-expression system harboring Pr-LFI and CF-DTE genes.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 14
·7760
·
IF 4.9
Churan Li,Boxiao Wu,Weihua Wang,Xiaoqin Yang,Yun Liu,Guolei Zhu,Sida Xie,Qian Jiang,Yong Ding,Yingjun Zhang,Ping Zhao,Lihua Zou
Arbutin and 6′-O-caffeoylarbutin (CA) fromVaccinium dunalianumWight are known for their ability to inhibit melanin synthesis. To boost the production of arbutin and CA, precursor feeding with hydroquinone (HQ) was studied inV. dunalianumsuspension cells. The effect of HQ on the biosynthesis of arbutin and CA in the suspension cells was investigated using high-performance liquid chromatography (HPLC), and possible molecular mechanisms were analyzed using metabolomics and transcriptomics analyses. HPLC analysis only showed that the addition of HQ significantly enhanced arbutin synthesis in cells, peaking at 15.52 ± 0.28 mg·g−1after 0.5 mmol·L−1HQ treatment for 12 h. Subsequently, metabolomics identified 78 differential expression metabolites (DEMs), of which arbutin and CA were significantly up-regulated metabolites. Moreover, transcriptomics found a total of 10,628 differential expression genes (DEGs). The integrated transcriptomics and metabolomics revealed that HQ significantly enhanced the expression of two arbutin synthase (AS) genes (Unigene0063512 and Unigene0063513), boosting arbutin synthesis. Additionally, it is speculated that CA was generated from arbutin and 3,4,5-tricaffeoylquinic acid catalyzed by caffeoyl transferase, with Unigene0044545, Unigene0043539, and Unigene0017356 as potentially associated genes with CA synthesis. These findings indicate that the precursor feeding strategy offers a promising approach for the mass production of arbutin and CA inV. dunalianumsuspension cells and provides new insights for CA biosynthesis inV. dunalianum.
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CHEMISTRY OF MATERIALS
·
2024
·
IF 7.2
Zhongyuan Li,Mengdi Xu,Wuqi Liu,Xuesong Wang,Ye Li,Xianju Zhou,Zhen Fang,Lixin Ning
Broadband near-infrared (NIR) luminescent materials hold the key to next-generation, smart, NIR light sources. The emerging halide perovskite materials are regarded as promising candidates for optoelectronics because of their superior optical properties and easy solution processability. However, it remains a major challenge to realize efficient and thermally stable broadband NIR luminescence in these materials. Herein, we report a novel strategy to achieve broadband NIR luminescence by utilizing the transitions from the lowest 1T2 level of Mo4+ to the components of its 3T1 ground state in the soft Cs2Zr(Cl,Br)6 lattice. The multiple transitions, each occurring within the (4d2) t22 crystal-field configuration and containing zero-phonon and vibronic contributions, superimpose on each other to generate the broadband NIR emission. The emission spectrum encompasses the long wavelength region from 800 to 1400 nm centered at 970 nm with a full width at half-maximum of 170 nm. More importantly, the title NIR phosphor shows an exceptional quantum efficiency (66.9%) and thermal stability (67%@150 °C). The as-fabricated NIR phosphor-converted light-emitting diode shows great potential as an NIR lighting source. This research opens a new horizon for realizing high-performance broadband NIR luminescence by exploiting multiple electronic transitions of activators in the soft lattice.
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Heliyon
·
2024
·
IF 3.4
Yu Yang,Xingyan Bao,Jiangyue Ning,Ruiyan Huang,Yuan Liang,Zelong Yan,Haotian Chen,Li Ding,Chang Shu
The investigation of peptide drugs has become essential in the development of innovative medications for hypertension. In this study, a sensitive high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method was developed to determine the plasma concentration and stability of the antihypertensive peptide FR-6 in rats. An isotopically labeled peptide (with an unchanged sequence) was utilized as an internal standard (IS) for validation purposes. Subsequently, this assay was employed to examine the pharmacokinetics of different administration methods (tail vein and gavage) in Sprague Dawley (SD) rats. Extracted plasma samples underwent sample preparation through methanol protein precipitation, followed by elution of FR-6 on Wondasil C18 Superb column (4.6 × 150 mm, 5 μm), using a mobile phase consisting of formic acid (0.1%) in water (A) and formic acid (0.125%)-ammonium formate (2 mM) in methanol (B). Ion pairs corresponding to FR-6 and IS were monitored via multiple reaction monitoring (MRM) under positive ion mode: m / z 400.7 → 285.1 for FR-6 and m / z 406.1 → 295.1 for IS detection respectively. The method exhibited excellent linearity with respect to FR-6 concentrations. In addition, the inter-day and intra-day precision were 0.61–6.85% and 1.76–11.75%; the inter-day and intra-day accuracy were −7.28–0.13% and −7.20–2.28%, respectively. In conclusion, the matrix effect, extraction recovery, and stability data were validated according to FDA recommended acceptance criteria for bioanalytical methods. This validated method serves as a reliable tool for determining the concentration of antihypertensive peptide FR-6, and has been successfully applied in pharmacokinetic studies involving rats.
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FEBS Open Bio
·
2024
·
IF 2.8
Linqian Chen,Meifeng Chen,Yan Xie,Yuyan Zhang,Shutian Mo,Yongfei He,Tianyi Liang,Yuan Liao,Renbin Huang,Guodong Huang,Chuangye Han,Thi Thai Hoa Pham
Several studies have suggested a potential antitumor effect of 2-dodecyl-6-methoxycyclohexa-2,5-diene-1,4-dione (DMDD). To further understand the mechanism of action of this compound, we investigated its effect on the phosphatidylinositol-3-kinase (PI3K)/serine–threonine kinase (Akt)/mammalian target of rapamycin (mTOR) signaling pathway. We show that DMDD application significantly inhibited the proliferation of breast cancer cell lines MDA-MB-231 and ER-α positive MCF-7. Furthermore, DMDD application resulted in increased intracellular reactive oxygen species (ROS) levels, apoptosis and autophagy, whereas it downregulated the expression of PI3K, Akt and mTOR mRNA and proteins, and increased the expression of LC3II/I and p62 proteins. In a mouse breast cancer xenograft model, DMDD inhibited tumor growth. Expression analyses suggest that ROS levels were higher in DMDD treated tumor tissues, whereas immunohistochemical analyses suggest that apoptotic cells were more prevalent in the DMDD treated group compared to the control group. Taken together, our results suggest that the molecular mechanism of action of DMDD may involve the enhancement of breast cancer autophagy through the PI3K/Akt/mTOR signaling pathway by mediating ROS expression.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2024
·
IF 2.6
Zhu Jie,Zhang Hua-nan,Wu Jia-kai,Li Fei-fei,Liu Yu-qing,Ning Er-juan,Yu Li-qin,Liang Shen
Camelina sativa oil (CSO) and Semen Ziziphi Spinosae oil (SZSO) are functional oils that have beneficial effects on brain health. This study evaluated the sedative and hypnotic effects of vegetable oils with various n − 6/n − 3 polyunsaturated fatty acids (PUFA) ratios and ɑ-linolenic acid (ALA) contents to mice. The n − 6/n − 3 PUFA ratios of CSO (CSO:SZSO = 1:0, 1.8 g/kg), SZSO (CSO:SZSO = 0:1, 1.8 g/kg), CSO-SZSO-L (CSO:SZSO = 1:1, 1.8 g/kg), and CSO-SZSO-H (CSO:SZSO = 1:1, 3.6 g/kg) were 0.51, 140, 1.69, and 1.69, respectively. The doses of ALA administered to mice with p-chlorophenylalanine-induced insomnia were approximately 0.64, 50 × 10 –4 , 0.32, and 0.64 g/kg, respectively. The mice were administered CSO, SZSO, and a low-dose combination of CSO and SZSO for seven days with no obvious hypnotic effects. However, the administration of a high-dose combination of CSO and SZSO significantly prolonged sleep duration in mice with induced insomnia and inhibited the serum levels of corticotropin-releasing hormone, adrenocorticotropic hormone, and cortisol. Interestingly, there were no significant effects on the structure and function of the hippocampal tissue. The results indicated that the anti-insomnia effects of these vegetable oils were positively correlated with a low n − 6/n − 3 PUFA ratio and the absolute amount of ALA.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Xin Ran,Qing Qu,Xingcan Qian,Wei Xie,Shunling Li,Lei Li,Long Yang
By combining the merits of water-soluble pillar[6]arenes (WP6s) and nitrogen-doped carbon quantum dots (N-CQDs), a sensitive electrochemical sensing platform was constructed by using the WP6-N-CQD nanocomposites as an electrode material for the detection of trinitrotoluene (TNT). N-CQDs were prepared via microwave route by using urea and citric acid as raw materials. Two kinds of macrocyclic hosts, β-CD and WP6, were incorporated on to the N-CQDs via hydrogen bonding and π − π interactions, respectively. The FTIR, TGA, and XPS characterizitions demonstrated that the β-CD-N-CQD and WP6-N-CQD composites were successfully prepared. The WP6-N-CQD-modified glass carbon electrode (GCE) indicated an enhanced electrochemical signal than the β-CD-N-CQDs/GCE in the reduction of 2,4,6-trinitrotoluene (TNT), which was attributed to the higher supramolecular recognition capability of the WP6 host than that of β-CD towards TNT. The linear ranges of 0.001 μM–1.0 μM and 1.0 μM–20.0 μM were obtained by using this electrochemical sensing platform. The detection limit was 0.95 nM (S/N = 3). The developed electrochemical sensing technique was successfully applied for the detection of TNT in lake water samples. Therefore, WP6-N-CQD nanocomposites are promising for the construction of high performance electrochemical sensors for the detection of explosives.
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POLYMER DEGRADATION AND STABILITY
·
2025
·
IF 7.4
Yanlu Sun,Shuo Fan,Xiulin Li,Xubin Wang,Dequn Wu,Xueli Wang,Jianyong Yu,Ruchao Yuan,Faxue Li
The limited carbon layer formation during combustion causes severe melt droplet formation and excessive heat release in polyamide 6 (PA6) filaments, hindering their application in textiles. In this work, phosphorus-containing transition metal complexes PAPH-M (M = Co, Ni, Zn) were synthesized by combining 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) with high oxidation state phosphorus and transition metal elements to enhance the carbonization effect of flame retardants in the PA6 matrix during combustion. By incorporating 10 wt% PAPH-Co into the PA6 matrix (PA6/PAPH-Co), a UL94 V-0 rating was achieved with no melt droplet. The peak of heat release rate (p-HRR) and total heat release (THR) of PA6/PAPH-Co decreased to 623 kW·m -2 and 56.8 MJ·m -2 , exhibiting reductions of 41.5% and 36.0%, respectively, compared to pure PA6. The exceptional flame retardancy, heat suppression and anti-dripping behaviors of PA6/PAPH-M composites were attributed to the quenching effect and barrier effect. Additionally, PA6/PAPH-M composites demonstrated exceptional UV-blocking capabilities, with ultraviolet protection factor (UPF) values of around 1900 compared to 3 for pure PA6. Melt-spun PA6/PAPH-Co filaments self-extinguished within 2 s in vertical burning tests and maintained sufficient mechanical strength for textile processing, confirming their sustained comprehensive performance post-spinning.
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危险品化学品经营许可证（带存储）