Frontiers in Cellular and Infection Microbiology
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2022
·
IF 6.073
Cui Huan,Zhang Cheng,Zhang Chunmao,Cai Zhuming,Chen Ligong,Chen Zhaoliang,Zhao Kui,Qiao Sina,Wang Yingchun,Meng Lijia,Dong Shishan,Liu Juxiang,Guo Zhendong
Influenza virus is a serious threat to global human health and public health security. There is an urgent need to develop new anti-influenza drugs. Lentinan (LNT) has attracted increasing attention in recent years. As potential protective agent, LNT has been shown to have anti-tumor, anti-inflammatory, and antiviral properties. However, there has been no further research into the anti-influenza action of lentinan in vivo, and the mechanism is still not fully understood. In this study, the anti-influenza effect and mechanism of Lentinan were studied in the Institute of Cancer Research (ICR) mouse model. The results showed that Lentinan had a high degree of protection in mice against infection with influenza A virus, delayed the emergence of clinical manifestations, improved the survival rate of mice, significantly prolonged the middle survival days, attenuated the weight loss, and reduced the lung coefficient of mice. It alleviated the pathological damage of mice infected with the influenza virus and improved blood indices. Lentinan treatment considerably inhibited inflammatory cytokine (TNF-α, IL-1β, IL-4, IL-5, IL-6) levels in the serum and lung and improved IFN-γ cytokine levels, which reduced cytokine storms caused by influenza virus infection. The underlying mechanisms of action involved Lentinan inhibiting the inflammatory response by regulating the TLR4/MyD88 signaling pathway. This study provides a foundation for the clinical application of Lentinan, and provides new insight into the development of novel immunomodulators.
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Nanoscale
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2022
·Vol. 14
·期 17
·6465-6470
·
IF 8.307
Zhixiang Li,Xueyan Feng,Wenping Hu,Lele Li
Apoptosis plays an important role in the life cycle of multicellular organisms. The development of techniques for sensitive monitoring of apoptosis-related key molecules can be used to assess not only disease progression but also its therapeutic interventions. However, there is still a lack of an imaging probe amenable for simultaneously detecting multiple biomarkers during drug-induced apoptosis. Herein, a novel activatable DNA nanodevice was designed to image apoptosis-related dual proteins in real time. The turn-on and specific recognition properties of our probe allow the spatially selective detection of apoptotic-related marker cytochrome c and apurinic/apyrimidinic endonuclease 1 in living cells. We demonstrated that the DNA nanodevice has the ability to monitor apoptosis and evaluate the efficacy of apoptosis-related drugs, which potentially can be used as a tool to evaluate the molecular mechanism of apoptosis regulation or to screen apoptotic drugs.
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Advanced Optical Materials
·
2022
·Vol. 10
·期 14
·2102709
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IF 10.05
Yinghua Liu,Jianfeng Zeng,Qing Li,Minqian Miao,Zhuorun Song,Min Zhao,Qingqing Miao,Mingyuan Gao
Developing smart molecular probes for assisting surgeons to precisely detect cancerous tissues and to completely remove all of them during the surgery is urgently required. Conventional fluorescent probes allow for tumor detection but with limited signal-to-noise ratio. Herein, an aminopeptidase N/CD13 (APN)-activated chemiluminescent probe (APN-ACLP) is reported for sensitive imaging and precise resection of malignant tumors. The APN-ACLP probe is based on acryl-substituted phenoxy-dioxetane that is coupled with an APN-cleavable substrate via a self-immolative linker. Upon activation by APN through specific hydrolysis reaction, APN-ACLP generates spontaneously chemiluminescence signal, which offers a possibility to sensitively search tumors by simply spraying APN-ACLP on the suspicious sites. Animal imaging studies reveal that a sufficient signal contrast between the cancerous tissues and the normal counterparts can be achieved within 5 min, which can allow for rapid detection and surgical resection of tumors, particularly tiny metastatic lesions in mice model, under the guidance of chemiluminescence imaging, well manifesting its potential for image-guided surgery of malignant tumors.
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MOLECULES
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2022
·Vol. 27
·期 9
·2629
·
IF 4.927
Hang Zhang,Xiaoxiao Chen,Shengliang Li,Jianliang Shen,Zong-Wan Mao
As hypoxia is closely associated with tumor progression, proliferation, invasion, metastasis, and strong resistance to therapy, regulating and overcoming the hypoxia tumor microenvironment are two increasingly important aspects of tumor treatment. Herein, we report a phototherapeutic platform that uses the organic photosensitizer diketopyrrolopyrrole (DPP) derivative and inorganic iridium salts (IrCl3) with photothermal activity and the capacity to decompose H2O2efficiently. The characterization of their photophysical properties proved that DPP-Ir nanoparticles are capable of remarkable near-infrared (NIR) absorption, and compared to DPP nanoparticles, the photothermal conversion efficiency (PCE) increases from 42.1% in DPP nanoparticles to 67.0% in DPP-Ir nanoparticles. The hybrid nanoparticles utilize the catalytic decomposition of endogenous H2O2to produce oxygen for the downregulation of the hypoxia-inducible factor 1 subunit alpha (HIF-1α) protein, which could reverse the tumor hypoxic microenvironment. Benefiting from the excellent optical properties and good biocompatibility, the hybrid platform exhibits efficient photothermal therapeutic effects as well as good biological safety. In conclusion, such a hybrid platform could improve photothermal therapy against cancer.Keywords:hypoxia;diketopyrrolopyrrole;photothermal therapy;catalyze H2O2;hybrid platform
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Journal of Materials Chemistry B
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2022
·Vol. 10
·期 40
·8274-8281
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IF 7.571
Yahui He,Zheng Zhou,Yuting Huang,Wenxiang Zhu,Ning He,Xiaolong Zhu,Xiaoxiao Han,Hairong Liu
The limited availability of bioinks has hindered the application of 3D bioprinting to tissue engineering, and bacterial infection is a serious threat to these applications. Aiming to solve this problem, a novel ε-poly-L-lysine (EPL)-derived antibacterial bioink has been developed for 3D bioprinting and tissue-engineering applications. Three glycidyl methacrylate (GMA)-modified EPL products, EPLGMA-1, EPLGMA-2, and EPLGMA-3, were prepared by reacting 3, 4, and 5 mL GMA with 5 g EPL, respectively. EPLGMA-1, EPLGMA-2, and EPLGMA-3 were photocurable and their corresponding photo-crosslinked hydrogels, EPLGMA-1H, EPLGMA-2H, and EPLGMA-3H, all exhibited very high antibacterial rates, good biocompatibility, good degradability, and promising mechanical properties. After EPLGMA-1H, EPLGMA-2H, and EPLGMA-3H with encapsulated chondrocytes were incubated for 4 weeks, EPLGMA-3H was the best one among them for tissue-engineering applications due to its most efficient tissue regeneration. Carrying chondrocytes, the EPLGMA-3 solution was printed into hydrogel products with high-fidelity shapes and high cell viability using a projection-based 3D bioprinter. Following the implantation of chondrocyte-loaded EPLGMA-3H samples into nude mice for 4 weeks, cartilage-like tissue was regenerated, suggesting that EPLGMA-3 is a promising antibacterial bioink for 3D bioprinting and tissue-engineering applications.
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Nano-Micro Letters
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2022
·Vol. 14
·期 1
·1-14
·
IF 23.655
Wu Wanbao,Bo Yiyang,Li Deping,Liang Yihong,Zhang Jichuan,Cao Miaomiao,Guo Ruitian,Zhu Zhenye,Ci Lijie,Li Mingyu,Zhang Jiaheng
Highlights
A novel amide-based nonflammable electrolyte is proposed. The formation mechanism and solvation chemistry are investigated by molecular dynamics simulations and density functional theory.
An inorganic/organic-rich solid electrolyte interphase with an abundance of LiF, Li 3 N and Li–N–C is in situ formed, leading to spherical lithium deposition.
The amide-based electrolyte can enable stable cycling performance at room temperature and 60 ℃.
Abstract The formation of lithium dendrites and the safety hazards arising from flammable liquid electrolytes have seriously hindered the development of high-energy-density lithium metal batteries. Herein, an emerging amide-based electrolyte is proposed, containing LiTFSI and butyrolactam in different molar ratios. 1,1,2,2-Tetrafluoroethyl-2,2,3,3-tetrafluoropropylether and fluoroethylene carbonate are introduced into the amide-based electrolyte as counter solvent and additives. The well-designed amide-based electrolyte possesses nonflammability, high ionic conductivity, high thermal stability and electrochemical stability (> 4.7 V). Besides, an inorganic/organic-rich solid electrolyte interphase with an abundance of LiF, Li 3 N and Li–N–C is in situ formed, leading to spherical lithium deposition. The formation mechanism and solvation chemistry of amide-based electrolyte are further investigated by molecular dynamics simulations and density functional theory. When applied in Li metal batteries with LiFePO 4 and LiMn 2 O 4 cathode, the amide-based electrolyte can enable stable cycling performance at room temperature and 60 ℃. This study provides a new insight into the development of amide-based electrolytes for lithium metal batteries.
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Journal of Environmental Sciences
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2022
·
IF 6.796
Xiao Chen,Yong Wang,Jianyu Li,Zhongheng Hu,Ying Zhou,Huayan Liu,Hanfeng Lu
The preparation of highly active supported noble metal catalysts with a low noble metal loading has always been the ultimate goal of researchers working on catalysis. Hydrothermally treated Pt/Al 2 O 3 (Pt/Al 2 O 3 -H) exhibits better catalytic activity than that (Pt/Al 2 O 3 -C) treated via the conventional calcination approach. At the high space velocity of 100,000 mL/(g∙hr), the temperature that correspond to 50% toluene conversion ( T 50 ) of Pt/Al 2 O 3 -H is 115°C lower than that of Pt/Al 2 O 3 -C, and the turnover frequency (TOF) value can reach 0.0756 sec −1 . The mechanism by which the hydrothermal approach enhances Pt/Al 2 O 3 activity has been investigated. The structure associated with the high catalytic activity of Pt nanoparticles (NPs) can be retained via hydrothermal treatment. Furthermore, the support is transformed to AlO(OH) with numerous surface hydroxyl groups, which in turn can facilitate the adsorption of toluene. And the synergistic effects of Pt NPs and AlO(OH) increases the contents of Pt in oxidation state and active oxygen, which are beneficial for toluene oxidation.
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CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Jun Zhang,Tingyang Xing,Min Zhang,Yunlong Zhou
Surface-enhanced Raman scattering (SERS) techniques benefit from the development of various substrates ranging from noble metal to semiconductor materials. Semiconductor substrates have been assumed to construct more specific structures and designable surface chemistry than noble metal substrates. Therefore, the development of semiconductor substrates may lead to the selective recognition of target molecules, more stable SERS spectra, and even low-cost fabrication of SERS substrates. In this paper, we disclose semiconductor supernanoparticles formed by a self-limiting assembly process with stable properties, easy scale-up fabrication, and high SERS enhancement. The detected concentration of analytes reaches as low as 10 −8 M. The built-in hotspots and self-doping in suprananoparticles provide strong electromagnetic enhancement, and the molecular resonance and charge transfer with the target molecule contribute to chemical enhancement. Discrete dipole approximation simulation was used to further confirm the SERS enhancement mechanism. Cu 2-x S supernanoparticles paved a new way to construct novel semiconductors with SERS activity.
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Advanced Materials Technologies
·
2022
·Vol. 7
·期 6
·2101131
·
IF 8.856
Cong Huang,Zhuang Hao,Ziran Wang,Hao Wang,Xuezeng Zhao,Yunlu Pan
An ultraflexible and transparent graphene-based field-effect transistors (GFET) wearable nanosensor is presented for detection of body fluid-biomarkers. The nanosensor is functionalized with a receptor that can specifically bind with the biomarker, resulting in a detectable change in the carrier concentration of the graphene. After recovery from 100 cycles of deformations (bending at radii 175 µm, folding at 150 ° , and shrinking at 50%), no visible mechanical damage is observed, and electrical properties of the nanosensor are also highly consistent. L-cysteine, associated with various diseases, is chosen as the biomarker to demonstrate the detection capability of the sensor. The nanosensor is capable of consistently and reliably detecting L-cysteine with a limit of detection of 0.022 × 10 −6 m in undiluted human sweat and 0.043 × 10 −6 m in artificial tears. The sensor is fabricated from the 1 µm transparent polyethylene glycol terephthalate substrate and WO 3 /Au/WO 3 electrode (transparency of 81%), and it can be mounted on the eyeball in tear detection without visual influence. These results demonstrate that the ultraflexible and transparent GFET wearable nanosensor can potentially be helpful in medical detection applications.
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Journal of Cleaner Production
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2022
·
IF 11.072
Lei Zhang,Xuexiu Jia,Yusen Ai,Renliang Huang,Wei Qi,Zhimin He,Jiří Jaromír Klemeš,Rongxin Su
Cellulose nanocrystals (CNCs) are usually prepared by sulphuric acid hydrolysis in industrial production, resulting in a large amount of alkaline and water consumption. In this paper, three acid-CNCs separation methods, including gravity settling, centrifugation and microfiltration , are used to directly separate sulphuric acid from hydrolysate mixture to reduce alkaline and water consumption. Microfiltration was selected as the best method for acid-CNCs separation. Using microfiltration, the highest sulphuric acid recovery ratio was about 65.0%, with an acid concentration range of 8–10 wt% in the pilot-scale CNCs production. Continuous centrifugation was introduced to replace gravity settling to separate CNCs from the neutralised mixture for faster production and reducing water consumption. The consumption of alkaline and water decreased by 63.0% and 68.0%, and the cost was reduced by 26%. The result of life cycle assessment (LCA) showed that the process with acid-CNCs separation using microfiltration achieved a reduction of 33.4% in aquatic ecotoxicity, 38.1% in terrestrial ecotoxicity , 47.9% in aquatic acidification, 55.8% in aquatic eutrophication , 40.0% in global warming potential and 47.8% in non-renewable energy consumptions. This study provided a promising approach for separating sulphuric acid from hydrolysate mixture and reducing consumption of alkaline and water. Such clean and economic process has great potential in the industrial production of CNCs.
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ACS Applied Polymer Materials
·
2022
·
IF 4.855
Yingjun Yang,Xiaoqiang Hou,Shufang Ma,Siyuan Huang,Jingyi Chen,Zejian Fang,Guanjian Nie,Bingshe Xu,Christophe A. Serra,Shukai Ding
AIEgens have attracted intensive interest because of the unusual fluorescence feature. Although AIEgen-based materials have been developed for responding to the diverse ex-stimuli, such as mechano-, electro-, and thermal-stimuli, the practical application of AIEgen-based thermoluminescence is hampered for the following reasons: (1) high cost attributed to the complicated chemical modification, (2) low contrast and responsiveness resulting from the chromophoric property of the thermal functional group. In this contribution, a facile encapsulation technology-based strategy without any chemical modifications is developed to form the encapsulated TPE nanogels for the preparation of the AIEgen-based thermoluminescence. Thanks to the coexistence of the liquid and solid phases, the nanogel-based AIEgens can not only attain the transparent feature but also a reversible transformation between the single-molecule and aggregated state by ex-thermal stimuli. In the nanogel, the two aggregated states of TPE molecules are observed that result from being tangled by poly-TPGDA and from being self-assembled, respectively. Furthermore, the nanogels prepared at different temperatures demonstrate the controllable fluorescence intensity. The higher preparation temperature results in more aggregated TPE and higher fluorescence intensity. Finally, the nanogels are easily formed into the transparent film thermometers because of their flowability, which shows a switchable and stable fluorescence by ex-thermal stimuli. The transparent film thermometer demonstrates high potential in anticounterfeiting technology and biological imaging.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2022
·Vol. 23
·期 4
·2080
·
IF 6.208
Zhaoqian Fan,Yukun Qin,Song Liu,Ronge Xing,Huahua Yu,Kecheng Li,Pengcheng Li
The exploration of novel, environmentally friendly, and efficient nematicides is essential, and modifying natural biomacromolecules is one feasible approach. In this study, 6-O-(trifluorobutenyl-oxadiazol)-chitosan oligosaccharide derivative was synthesized and characterized by FTIR, NMR, and TG/DTG. Its bioactivity and action mode against root-knot nematodeM. incognitawere estimated. The results show that the derivative shows high nematicidal activity against J2s, and egg hatching inhibitory activity at 1 mg/mL. The derivative may affect nematode ROS metabolism and further damage intestinal tissue to kill nematode. Meanwhile, by synergism with improving crop resistance, the derivative performed a high control effect on the nematode with low phytotoxicity. These findings suggested that chitosan oligosaccharide derivatives bearing fluoroalkenyl groups are promising green nematicides.Keywords:chitosan oligosaccharide;nematicide;Meloidogyne incognita;trifluorobutenyl group
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危险品化学品经营许可证（带存储）