JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-14
·
IF 10.2
Zhao Yitong,Bo Tao,Wang Chenfei,Yao Dingjin,Pan Chaolan,Xu Weiyi,Zhou Hao,Li Ming,Zhang Si
Extensive efforts have been dedicated to enhancing the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in cancer cells for the development of effective cancer treatments. However, highly safe and efficient delivery of TRAIL gene remains a significant challenge, especially using cationic polymers. Here, a series of highly branched-linear poly(β-amino ester)s (H-LPAEs) are developed through a unique oligomer branching strategy. H-LPAEs exhibit a more uniform distribution of linear segments and branching units, leading to excellent DNA condensation and favorable physicochemical properties of H-LPAE/DNA polyplexes. In SW1353 and BMSC cells, the optimized H-LPAEs, H-LPAE B4−S5−TMPTA , achieves superior gene transfection efficiency of 58.0% and 33.4%, which were 2.5-fold and 2.0-fold higher than that of the leading commercial gene transfection reagent, Lipofectamine 3000. Excitingly, H-LPAE B4−S5−TMPTA mediated 56.7% and 28.1% cell apoptosis in HepG2 cells and HeLa cells highlighting its potential application in cancer gene therapy. In addition, locally administered H-LPAE B4−S5−TMPTA delivered TRAIL DNA to HepG2 xenograft tumors and inhibited tumor growth in vivo. This study not only proposes a novel strategy for synthesizing poly(β-amino ester)s with a unique branched-linear topology but also identifies a promising candidate for highly efficient TRAIL gene transfection.
研究领域:
癌症研究
·
基因治疗
·
聚合物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Small Methods
·
2025
·Vol. 9
·期 12
·e01317
·
IF 9.1
De'en Guo,Yi Yang,Qingrui Cai,Bingchen He,Xingyu Gao,Heng Peng,Junhao Xue,Conghua Zhou
Carbon- electrode (CE) based, hole-conductor-free mesoscopic perovskite solar cells (meso-CPSCs) offer the merits of high stability and low cost, but they contain a large number of nanosized perovskite (PVSK) crystallites in the CE region. These crystallites contribute little to light absorption, but bring risks of charge recombination. Herein, a three-step holistic treatment is introduced to address this issue, such as treatment-1 (T1): partial CE peeling; treatment-2 (T2): octylammonium iodide modification; and treatment-3 (T3): coating of a secondary layer of CE. Low-temperature meso-CPSCs are chosen as the model platform to perform the strategy. It is observed that the strategy removes the redundant PVSK crystallites, induces the formation of tilt-stacked two-dimensional (2D) PVSK, and strengthens interfacial contact of PVSK/CE, which reduces the recombination risks and facilitates interfacial charge-extraction, and finally elevates the power conversion efficiency (PCE) of the low-temperature meso-CPSCs from 14.25(±0.69)% to 16.05(±0.40)%(optimized to 17.28%). The efficiency is further upgraded to 18.07% after additional optimization on internal resistance and substrate transparency. Such performance achieves an increment of ≈50% when comparing to the efficiency of ≈12% that was firstly reported in 2020. In more, the T 80 lifetime of ≈525 h has been obtained during the quasi-maximum power point tracking test in open air (device unsealed).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Di Wang,Wenqi Wang,Bijun Zhao,Jiayun Liang,Lingzhu Song,Yixuan Chen,Wushuang Bai
A novel electrochemical–visual colorimetric AFB1 biosensing method was proposed. • The sensing method achieves spatial separation of AFB1 recognition and detection. • The electrochemical mode achieves low LOD of 4.2 × 10 −7 ng/mL and wide linear range. • The colorimetric modal achieves low LOD of 3.4 × 10 −7 ng/mL and wide linear range. • This work proposes a general dual-mode trace-level targets biosensing strategy. Aflatoxin B1 (AFB1) can be present in moldy grains, nuts, oil crops, and their processed by-products, posing a threat to food safety and human health. Therefore, sensitive and efficient detection of trace AFB1 is of great significance for early intervention in protection of food safety. In this research, an aptamer was used as the recognition agent for AFB1, a novel magnetically controlled electrochemical-visual colorimetric dual-mode AFB1 biosensing strategy was developed. H-shaped device consisting of a left cell and a right cell was designed, with the anodic aluminum oxide (AAO) membrane nanochannel in between. Under magnetic control, specific recognition of AFB1 by the aptamer in the left cell triggers DNA hybridization chain reaction (HCR) in the right cell, forming a glucose oxidase and peroxidase-like DNA G-quadruplex cascade catalytic structure, enabling the determination of AFB1 via glucose catalysis. This method incorporates dual signal amplification technology (HCR and enzyme cascade catalytic) and achieves spatial separation between AFB1 recognition and detection, thereby effectively reducing background interference and improving sensitivity. Using the electrochemical mode (Differential pulse voltammetry), the method achieved low limit of detection (LOD) of 4.2 × 10 −7 ng/mL, wide linear range of 10 −6 -10 2 ng/mL, excellent selectivity and anti-interference performance. Using the visual colorimetric mode, the LOD was as low as 3.4 × 10 −7 ng/mL and the linear range was 10 −6 -10 4 ng/mL. This work proposes a general dual-mode biosensing strategy and provides a promising approach for highly sensitive and efficient detection of trace-level targets in complex samples.
✓ 已验证
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2022
·Vol. 20
·期 1
·1-16
·
IF 9.429
Liu Jie,Hu Xiaochun,Feng Lei,Lin Yun,Liang Shujing,Zhu Zhounan,Shi Shuo,Dong Chunyan
Background Non-redundant properties such as hypoxia and acidosis promote tumor metabolic adaptation and limit anti-cancer therapies. The key to the adaptation of tumor cells to hypoxia is the transcriptional and stable expression of hypoxia-inducible factor-1 alpha (HIF-1α). The phosphorylation-activated tumorigenic signal PI3K/AKT/mTOR advances the production of downstream HIF-1α to adapt to tumor hypoxia. Studies have elucidated that acid favors inhibition of mTOR signal. Nonetheless, carbonic anhydrase IX (CAIX), overexpressed on membranes of hypoxia tumor cells with pH-regulatory effects, attenuates intracellular acidity, which is unfavorable for mTOR inhibition. Herein, a drug delivery nanoplatform equipped with dual PI3K/mTOR inhibitor Dactolisib (NVP-BEZ235, BEZ235) and CAIX inhibitor 4‐(2‐aminoethyl) benzene sulfonamide (ABS) was designed to mitigate hypoxic adaptation and improve breast cancer treatment. Results ABS and PEG-NH 2 were successfully modified on the surface of hollow polydopamine (HPDA), while BEZ235 and Chlorin e6 (Ce6) were effectively loaded with the interior of HPDA to form HPDA-ABS/PEG-BEZ235/Ce6 (H-APBC) nanoparticles. The release of BEZ235 from H-APBC in acid microenvironment could mitigate PI3K/mTOR signal and resist HIF-1α-dependent tumor hypoxia adaptation. More importantly, ABS modified on the surface of H-APBC could augment intracellular acids and enhances the mTOR inhibition. The nanoplatform combined with phototherapy inhibited orthotopic breast cancer growth while reducing spontaneous lung metastasis, angiogenesis, based on altering the microenvironment adapted to hypoxia and extracellular acidosis. Conclusion Taken together, compared with free BEZ235 and ABS, the nanoplatform exhibited remarkable anti-tumor efficiency, reduced hypoxia adaptation, mitigated off-tumor toxicity of BEZ235 and solved the limited bioavailability of BEZ235 caused by weak solubility. Graphical Abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 34
·5463-5474
·
IF 5.342
Wuhou Fan,Yong Jin,Liangjie Shi
Achieving the room-temperature self-healing of polymer materials with mechanically robust and tough features is a challenging project due to their conflicting attributes. Herein, a novel design concept of dynamic but mechanically robust and tough polymer networks was proposed derived from dynamic diselenide bonds and dual H-bonds, in which flexible fluorinated siloxane units and H-bonded urethane and urea groups are embedded simultaneously. Through adjustment of the mass fraction of fluorinated siloxane units incorporated, waterborne polyurethanes with good mechanical performances (tensile strength: 16.31 MPa; toughness: 69.8 MJ m−3) could be constructed. Meanwhile, this optimized sample could also be healed at room-temperature within 2 h under visible light. Therefore, the fast room-temperature self-healing of our elaborated polymers with mechanically robust and tough features is achieved, which has never been achieved for the contemporary polymer materials. The reconstruction of dual H-bonding interactions of urethane and urea moieties is responsible for the prophase healing after reconnecting the fracture surfaces, and macromolecular chains containing fluorinated siloxane units with excellent flexibility could promote the visible-light-triggered diselenide metathesis in a highly efficient manner subsequently. Moreover, much improved water resistance properties could also be achieved. The elaborated strategy will provide inspiration for developing mechanically robust and tough water-dispersed polymer materials with fast room-temperature self-repairing performance and desirable water resistance properties, which are of vital importance to the next generation of smart materials.
✓ 已验证
DOI
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2017
·
IF 4.075
Zheng Su,Hua Wang,Xianzhu Ye,Konghu Tian,Weiqi Huang,Yulan Guo,Jing He,Xingyou Tian
Thermally conductive poly (2-ethylhexyl acrylate) (P2EHA)/functionalized graphene/h-boron nitride composites materials were fabricated by colloid blending and self-assembly approach. Here, self-assembly technology has been used to achieve the facile surface modification of hexagonal boron nitride (h-BN) microplatelets by forming a functionalized graphene (f-G) sheets on its surface via π-π interaction. The polar functionality on the graphene surface allowed the permeation of the polymer matrix through the secondary interaction between them, increasing the content of this hybridized filler in composite. The effective and successful fabrication of f-G@h-BN microplates have been confirmed by SEM, Raman spectroscopy , XRD , and TGA investigations. The self-alignment 2D stacked fillers result in higher in-plane thermal conductivities and excellent electrical insulation of the composites. In addition to the good adhesive properties , the procedure is environment-friendly, easy operation, and potential for the practical application in large scale.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2024
·
IF 10.7
Miao Wang,Yijin He,Xiaoyu Yang,Xuhui Hou,Wenxuan Li,Shaobo Tan,Zhicheng Zhang
With the increasing demand for dielectric materials in high-frequency microwave applications, fluoropolymer-based dielectric materials have been widely utilized due to their excellent dielectric and insulation properties. However, the low thermal conductivity limits their ability to meet the thermal dissipation requirements during high-frequency and high-speed signal transmission. The present study introduces a pioneering interface engineering strategy wherein C–F bonds on the surface of ethylene-tetrafluoroethylene copolymer (ETFE) are photo-catalytically activated, enabling successful grafting of poly(glycidyl methacrylate) (PGMA) chain segments. Subsequently, the resulting graft copolymers are blended with thermally conductive h-BN filler to prepare composite dielectric materials. The results demonstrate that the incorporation of PGMA segments significantly enhances the interfacial compatibility between the ETFE matrix and h-BN filler, resulting in composites with a dense microstructure and outstanding dielectric and thermal properties. At a filler content of 30 vol%, the ETFE-g-PGMA@BN composite achieves an in-plane thermal conductivity of 4.2 W (m K)−1 and a through-plane thermal conductivity of 0.77 W (m K)−1. Moreover, at 1 GHz, the composite exhibits a dielectric constant of 2.2 and a dielectric loss of 0.004. This work presents a novel interface design strategy for surface modification of PTFE-based fluoropolymers, expanding their potential applications in printed circuit boards (PCBs) and providing a new direction for the design of polymer-based dielectric materials.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Cong Wan,Jianwen Zhang,Honglei Zhang,Min Liu,Yiming Li,Zijun Yan,Chengheng Pang,Tao Wu
The catalytic reduction of CO₂ into valuable chemicals and fuels is a promising strategy for mitigating global carbon emissions. This study explores the synergistic co-reduction of CO₂ and H₂O using nanoscale zero-valent iron (NZVI) as a dual-function catalyst. Under light irradiation at 300 °C, the CO₂ reduction rate increased by more than 10-fold compared to dark conditions, demonstrating significant photothermal synergy. The study reveals that NZVI undergoes a phase transformation, where Fe₃O₄ functions as a photocatalyst while Fe 0 serves as an electron donor, enabling a unique dual-functionality that facilitates efficient electron transfer. The addition of 10 vol% H₂ stabilizes the reaction, leading to a higher and more consistent CO yield. When investigating the simultaneous reduction of CO₂ and H₂O, we discovered competitive adsorption and reaction pathways that significantly influence product distribution. In-situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) and density functional theory (DFT) calculations revealed three distinct mechanistic pathways for CO₂ conversion: direct C O bond cleavage, a carboxylate-mediated pathway and bicarbonate-mediated pathway, with the carboxylate and bicarbonate pathways being uniquely promoted by H and OH from H₂O dissociation. The addition of H₂O into the CO₂ and H₂ reaction significantly enhanced CH₄ generation due to the extra H source from H₂O dissociation. These findings offer valuable insights into the competitive mechanistic pathways of CO₂-H₂O co-reduction and contribute to the design of more efficient iron-based catalysts for sustainable CO₂ conversion and water splitting in energy and environmental applications.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuzhuang Sun,Bocheng Yu,Yu Liu,Bin Lu,Yanmei Shen,Yanmin Zhang,Hongman Sun,Hui Zhou,Hengshan Qiu
Integrated carbon capture and utilization via dry reforming of methane (ICCU-DRM) paves a sustainable way for directly converting CO 2 from flue gas into CO. However, the process suffers from severe carbon depositions and low CH 4 utilization. Herein, we demonstrate that the NiMnxCa dual functional materials undergo a spontaneous structural self-optimization during cyclic ICCU-DRM process. The MnO x can segregate from (MnO) α (CaO) 1-α solid solution during CO 2 capture. Consequently, the Ni-MnO x interaction, with a MnO x -coated Ni surface, can be intensified with the material reconstruction, which effectively suppresses the hydrogen recombination probability and prevent carbon deposition. Moreover, the *H from CH 4 activation can react with MnO, enabling highly efficient conversion of CO 2 via in situ chemical looping pathway. As an example, the NiMn5Ca achieved a ~ 100 % H utilization (~ 0 H 2 production), 95 % CO 2 conversion and < 1 carbon deposition/per mol of converted CO 2 at 675 °C in 25 cycles. The work illustrates a typical scenario of an intelligent structure optimization under reaction environment and proposes a strategy to enhance CH 4 utilization efficiency by directing *H to a fast surface reaction rather than desorbing as H 2 .
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2015
·Vol. 140
·期 13
·4566-4575
·
IF 4.107
Jacky F. C. Loo,S. S. Wang,F. Peng,J. A. He,L. He,Y. C. Guo,D. Y. Gu,H. C. Kwok,S. Y. Wu,H. P. Ho,W. D. Xie,Y. H. Shao,S. K. Kong
As in all RNA viruses, influenza viruses change and mutate constantly because their RNA polymerase has no proofreading ability. This poses a serious threat to public health nowadays. In addition, traditional pathogen-based detection methods may not be able to report an infection from an unknown type or a subtype of virus if its nucleotide sequence is not known. Because of these factors, targeting host microRNA signatures may be an alternative to classify infections and distinguish types of pathogens as microRNAs are produced in humans shortly after infection. Although this approach is in its infant stage, there is an urgent need to develop a rapid reporter assay for microRNA for disease control and prevention. As a proof of concept, we report herein for the first time a non-PCR MARS (MicroRNA-RNase-SPR) assay to detect the microRNA miR-29a-3p from human subjects infected with influenza virus H1N1 by surface plasmon resonance (SPR). In our MARS assay, RNase H is employed to specifically hydrolyze the RNA probes immobilized on the gold surface where they hybridize with its cognate target cDNAs miR-29a-3p, where it was formed from reverse transcription with mature miR-29a-3p specific stem-looped primers. After the digestion of the RNA probe by RNase H, the intact cDNA was released from the RNA–DNA hybrid and bound to a new RNA probe for another enzymatic reaction cycle to amplify signals. With assay optimization, the detection limit of our MARS assay for miR-29a-3p was found to be 1 nM, and this new assay could be completed within 1 hour without thermal cycling. This non-PCR assay with high selectivity for mature microRNA provides a new platform for rapid disease diagnosis, quarantine and disease control.
✓ 已验证
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2021
·
IF 2.545
Wenting Chen,Hua Liang,Xin Wen,Zongchen Li,Hui Xiong,Qiang Tian,Minhao Yan,Yuanzhi Tan,Guy Royal
To explore the effect of sensor structure on anions detection, three new hydrazones P-CN , P-CF 3 and P-NO 2 having different electron-withdraw groups are synthesized. Their anions recognition properties are investigated compared with those of P-OMe , P-H and acylhydrazone B-NO 2 . It shows all non-nitro hydrazones exhibit increasing sensitivity specifically toward CN − in the order of P-OMe < P-H < P-CN < P-CF 3 , indicated by color change from colorless to different shades of blue. Whereas P-NO 2 responds to CN − , F − and H 2 PO 4 − concurrently and its sensitivity to CN − is more than 4 times higher compared with that of the other hydrazones. CN − can be discriminated from F − by P-NO 2 in acetonitrile/water. On the other hand, B-NO 2 has only discernible response to CN − and F − with less sensitivity than P-NO 2 does. Detection details of P-NO 2 to CN − are investigated by titration, anti-interference tests, Job’s plot, HRMS, pH effect assays. The 1 H NMR, IR and reversibility tests demonstrate the deprotonation mechanism. The detection mechanisms of P-NO 2 and B-NO 2 to CN − are compared and demonstrate the reason of their recognition difference. The detection limits of P-NO 2 to CN − , F − and H 2 PO 4 − in acetonitrile are 0.0477 μM, 0.0876 μM and 1.46 μM, respectively. Hence, this work indicates that strong electron-withdraw group in related probes can improve their anion detection ability, meanwhile conjugation effect should be also considered in structure designing.
研究领域:
分析化学
·
材料科学
·
化学传感器
✓ 已验证
DOI
高影响因子
Science Advances
·
2022
·Vol. 8
·期 23
·
IF 14.957
Peixin Zhang,Yao Zhong,Yan Zhang,Zhenliang Zhu,Yuan Liu,Yun Su,Jingwen Chen,Shixia Chen,Zheling Zeng,Huabin Xing,Shuguang Deng,Jun Wang
One-step separation of C 2 H 4 from ternary C 2 H 2 /C 2 H 4 /C 2 H 6 hydrocarbon mixtures is of great significance in the industry but is challenging due to the similar sizes and physical properties of C 2 H 2 , C 2 H 4 , and C 2 H 6 . Here, we report an anion-pillared hybrid ultramicroporous material, CuTiF 6 -TPPY, that has the ability of selective recognition of C 2 H 4 over C 2 H 2 and C 2 H 6 . The 4,6-connected fsc framework of CuTiF 6 -TPPY exhibits semi–cage-like one-dimensional channels sustained by porphyrin rings and TiF 6 2− pillars, which demonstrates the noticeably enhanced adsorption of C 2 H 2 and C 2 H 6 over C 2 H 4 . Dynamic breakthrough experiments confirm the direct and facile high-purity C 2 H 4 (>99.9%) production from a ternary gas mixture of C 2 H 2 /C 2 H 6 /C 2 H 4 (1/9/90, v/v/v) under ambient conditions. Computational studies and in situ infrared reveal that the porphyrin moieties with large π-surfaces form multiple van der Waals interactions with C 2 H 6 ; meanwhile, the polar TiF 6 2− pillars form C–H•••F hydrogen bonding with C 2 H 2 . In contrast, the recognition sites for C 2 H 4 in the framework are less marked.
研究领域:
材料科学
·
化学工程
·
物理化学
·
吸附分离
·
多孔材料
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）