TALANTA
·
2017
·
IF 4.162
Lan Luo,Fengzhou Xu,Hui Shi,Xiaoxiao He,Taiping Qing,Yanli Lei,Jinlu Tang,Dinggeng He,Kemin Wang
Deoxyribonuclease I (DNase I) is an important physiological indicator and diagnostic biomarker, but traditional methods for assessing its activity are time-consuming, laborious, and usually radioactive. Herein, by effectively combining the special functions of DNase I and terminal deoxynucleotidyl transferase (TdT), a simple, green, cost-effective, label-free and ultrasensitive assay for DNase I activity has been constructed based on superlong poly(thymine)-hosted copper nanoparticles (poly T-CuNPs). In this strategy, a 3′-phosphorylated DNA primer is designed to block TdT polymerization. After addition of DNase I, the primer could be digested to release 3′-hydroxylated fragments, which could further be tailed by TdT in dTTP pool with superlong poly T ssDNA for CuNPs formation. Fluorescence measurements and gel electrophoresis demonstrated its feasibility for DNase I analysis. The results indicated that with a size of 3–4 nm, the CuNPs templated by TdT-polymerized superlong poly T (>500 mer) had several advantages such as short synthetic time (<5 min), large Stokes shift (~275 nm) and intense red fluorescence emission . Under the optimal conditions, quantitative detection of DNase I was realized, showing a good linear correlation between 0.02 and 2.0 U/mL (R 2 =0.9928) and a detection limit of 0.02 U/mL. By selecting six other nucleases or proteins as controls, an excellent specificity was also verified. Then, the strategy was successfully applied to detect DNase I in diluted serum with a standard addition method, thus implying its reliability and practicability for biological samples. The proposed strategy might be promising as a sensing platform for related molecular biology and disease studies.
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Frontiers in Plant Science
·
2017
·
IF 4.291
Chen Guilin,Guo Mingquan
Topoisomerase I (Topo I) catalyzes topological interconversion of duplex DNA during DNA replication and transcription, and has been deemed as important antineoplastic targets. In this study, the fraction R.d-60 from ethyl acetate extracts of Rhamnus davurica showed higher inhibitory rates against SGC-7901 and HT-29 compared with the R.d-30 fraction in vitro. However, the specific active components of R.d-60 fraction remain elusive. To this end, a method based on bio-affinity ultrafiltration and high performance liquid chromatography/electrospray mass spectrometry (HPLC- ESI-MS/MS) was developed to rapidly screen and identify the Topo I inhibitors in this fraction. The enrichment factors (EFs) were calculated to evaluate the binding affinities between the bioactive constituents and Topo I. As a result, eight ligands were identified and six of which with higher EFs showed more potential antitumor activity. Furthermore, antiproliferative assays in vitro (IC50 values) with two representative candidates (apigenin, quercetin) against SGC-7901, HT-29 and Hep G2 cells were conducted and further validated. Finally, the structure-activity relationships revealed that flavones contain a C2-C3 double bond of C ring exhibited higher bio-affinities to Topo I than those without it. This integrated method combining Topo I ultrafiltration with HPLC-MS/MS proved to be very efficient in rapid screening and identification of potential Topo I inhibitors from the complex extracts of medicinal plants, and could be further explored as a valuable high-throughput screening platform in the early drug discovery stage.
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JOURNAL OF MATERIALS SCIENCE
·
2016
·Vol. 51
·期 23
·10574-10584
·
IF 2.302
Zhao Jie,Xie Yu,Fang Jing,Ling Yun,Gao Yunhua,Liu Xiaoming,Zhang Qiugen,Xu Qiuhua,Xiong Hui
A novel electromagnetic material has been synthesized through P -phenylenediamine-benzoquinone (PB) Schiff base Ag(I) complexes chemically bonded to few-layered graphene (FLG). The structure of the samples was characterized by Fourier transform infrared spectroscopy and X-ray diffraction. The shape and size were observed by scanning electron microscopy and transmission electron microscopy. The conductive properties were tested by a four-probe conductivity tester at room temperature, and the magnetic properties of the samples were tested with the vibrating sample magnetometer. These results show that the PB Schiff base Ag(I) complexes are chemically bonded onto the FLG layers. The conductivity, magnetic saturation, residual magnetization, and coercive force of the composite achieve 5.86 S cm −1 , 1.47 emu g −1 , 0.26 emu g −1 , and 208.3 Oe, respectively. Finally, based on the experimental results, the probable formation, electronic transmission, and magnetic mechanism have also been investigated.
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CHINESE JOURNAL OF CATALYSIS
·
2016
·
IF 2.628
Xinping Duan,Yan Yin,Xuelin Tian,Jinhuo Ke,Zhaojun Wen,Jianwei Zheng,Menglin Hu,Linmin Ye,Youzhu Yuan
Disintegration or redispersion of supported sintered gold nanoparticles (Au NPs) in the presence of alkyl halide can give catalyst regeneration or redispersion of sintered Au catalysts. The selectivity of alkyl halides, temperature and size distributions were investigated to elucidate the redispersion of Au NPs during halide-induced decomposition. This study proved that the alkyl halide induced the redispersion of sintered Au NPs which depended on the R–X (X = I, Br, Cl) bond dissociation energy (BDE) and thus provided a simple descriptor for the regeneration of inactive supported Au catalysts. A correlation between the BDE of R–X and dispersion efficiency was established. The tendency for disintegration and redispersion followed the R–X BDE of the alkyl halide. Compared to alkyl chlorides and bromides, iodides were more efficient for redispersing sintered Au NPs. As a descriptor, the BDE of R–I played a crucial role in particle redispersion. These findings provided insights into the mechanism of organic halide-induced Au NP disintegration and the effect of the halide type on the redispersion of sintered catalysts.
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JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Lu Liu,Yiwen Wu,Mengyu Guan,Zhigao Dai,Guogang Li
The luminescence properties of perovskite quantum dots are crucial for their applications in various optoelectronic devices. Enhancing the luminescence performance of CsPb(Br/I) 3 quantum dots is a critical challenge for their application in optoelectronic devices. This paper explores the use of noble metal nanostructures to improve the luminescence intensity of these quantum dots through localized surface plasmon resonance (LSPR). We conducted electric field simulations to optimize the LSPR effect and prepared Au@Ag@SiO 2 nanobipyramids with tailored LSPR peaks. The nanobipyramids were then connected to CsPbCl 2 Br, CsPbBr 3 , and CsPbBrI 2 quantum dots using amine-functionalized SiO 2 layers, forming a nanocomposite that showed a significant increase in luminescence. The study identifies the importance of the SiO 2 spacer layer in preventing luminescence quenching and demonstrates that the LSPR effect can be harnessed to achieve up to a 15-fold enhancement in luminescence intensity. This research provides a promising avenue for advancing the efficiency of solar energy harvesting devices, light-emitting devices, and optical detectors.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2024
·Vol. 39
·期 1
·e7959
·
IF 3.7
Zhuoqin Li,Minghui Ma,Zhiyin Xiao,Xiujuan Jiang,Xinlei Xu,Hailong Wang,Peter C. Ford,Xiaoming Liu
Five novel manganese (I) tricarbonyl complexes were synthesized by the reaction of Mn (CO) 5 Br with quinoline-2-carboxaldehyde and different anilines. The IR, UV, and myoglobin results indicate that these complexes can be used as photoCORMs (photo photoinduced carbon monoxide releasing molecules) activated by red light. The visible range spectra display strong charge transfer (CT) bands, the maxima of which are sensitive to the ligand substituents. Density functional theory (DFT) and time-dependent density functional theory (TDDFT) computations show that these CT transitions are admixtures of metal-to-ligand and aniline-to-quinoline charge transfers and that increasing the electron-donating ability on the aniline functionality can lead to shifts in the absorption bands of the corresponding compounds to longer wavelengths and to enhanced band intensities, thus enhancing their photoactivity. Photolysis of these complexes in aerobic solution leads to CO photodissociation followed by oxidation of the Mn(I) center to Mn(II).
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xuan He,Dan Liang,Jun Zhou,Kangjing Li,Beibei Xie,Chunyun Liang,Cong Liu,Zhiyong Chen,Xinxin Chen,Ao Long,Shufang Zhuo,Xiaoping Su,Ying Luo,Wenxia Chen,Fengfeng Zhao,Xinglu Jiang
The cell nucleus serves as the pivotal command center of living cells, and delivering therapeutic agents directly into the nucleus can result in highly efficient anti-tumor eradication of cancer cells. However, nucleus-targeting drug delivery is very difficult due to the presence of numerous biological barriers. Here, three antitumor drugs (DNase I, ICG: indocyanine green, and THP: pirarubicin) were sequentially triggered protein self-assembly to produce a nucleus-targeting and programmed responsive multi-drugs delivery system (DIT). DIT consisted of uniform spherical particles with a size of 282 ± 7.7 nm. The acidic microenvironment of tumors and near-infrared light could successively trigger DIT for the programmed release of three drugs, enabling targeted delivery to the tumor. THP served as a nucleus-guiding molecule and a chemotherapy drug. Through THP-guided DIT, DNase I was successfully delivered to the nucleus of tumor cells and killed them by degrading their DNA. Tumor acidic microenvironment had the ability to induce DIT, leading to the aggregation of sufficient ICG in the tumor tissues. This provided an opportunity for the photothermal therapy of ICG. Hence, three drugs were cleverly combined using a simple method to achieve multi-drugs targeted delivery and highly effective combined anticancer therapy.
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Advanced Healthcare Materials
·
2025
·
IF 9.6
Minggang Yang,Yufan Zhang,Zhenhao Hou,Jianbo Wu,Fuwei Liu,Jun Wu,Kelvin W.K. Yeung,Wenhao Qian,Xuanyong Liu,Liang Kong,Yunpeng Li,Jiajun Qiu,Guocheng Wang
Antibiotic therapy is a key strategy for treating infections associated with orthopedic implants, yet its limited effectiveness and potential to disrupt bone healing highlight the need for innovative approaches. Herein, a TiO 2 -Graphene (TiO 2 -G) metastructure is developed on Ti implant surface using a hydrothermal method coupled with plasma-enhanced chemical vapor deposition (PECVD), showing strong near-infrared light (NIR) absorption. The antibiotic doxycycline (DOX) is successfully loaded onto TiO 2 -G and exhibited enhanced NIR release. The promising antibacterial efficacy is proven by both in vitro and in vivo tests with NIR irradiation for 5 min, which is ascribed to the synergistic photocatalytic activity of TiO 2 -G and NIR-responsive release of DOX. Interestingly, after 5 min of NIR irradiation, the TiO 2 -G metasurface neutralized the immediate negative effects of photodynamics and even upregulated the expression of osteogenic genes (osteocalcin (OPN), osteopontin (BSP), and bone sialoprotein (OCN)), with particularly enhanced effects observed on day 14. Moreover, the sustained release function of TiO 2 -G significantly mitigated the cytotoxicity of free antibiotics with antibacterial capabilities comparable to those of TiO 2 -G/DOX under NIR irradiation for 5 min. Consequently, the in vivo studies proved that the TiO 2 -G metastructure enhanced the osteointegration of the implant even in the absence of infection when loaded with DOX.
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Foods
·
2024
·Vol. 13
·期 4
·625
·
IF 4.7
Ding-Tao Wu,Jing Lei,Jie Li,Mei-Mei Qu Mo,Wen-Bing Li,Yu-Jing Huang,Yi-Chen Hu,Ai-Li Wang,Liang Zou
Tartary buckwheat green leaves are considered to be among the most important by-products in the buckwheat industry. Although Tartary buckwheat green leaves are abundant in pectic polysaccharides, their potential applications in the food industry are quite scarce. Therefore, to promote their potential applications as functional or fortified food ingredients, both deep-eutectic-solvent-assisted extraction (DESE) and high-pressure-assisted deep eutectic solvent extraction (HPDEE) were used to efficiently and selectively extract pectic polysaccharides from Tartary buckwheat green leaves (TBP). The results revealed that both the DESE and HPDEE techniques not only improved the extraction efficiency of TBP but also regulated its structural properties and beneficial effects. The primary chemical structures of TBP extracted using different methods were stable overall, mainly consisting of homogalacturonan and rhamnogalacturonan-I (RG-I) pectic regions. However, both the DESE and HPDEE methods could selectively extract RG-I-enriched TBP, and the proportion of the RG-I pectic region in TBP obviously improved. Additionally, both the DESE and HPDEE methods could improve the antioxidant and anti-glycosylation effects of TBP by increasing its proportion of free uronic acids and content of bound polyphenolics and reducing its molecular weight. Moreover, both the DESE and HPDEE methods could partially intensify the immunostimulatory effect of TBP by increasing its proportion of the RG-I pectic region. These findings suggest that DES-based extraction techniques, especially the HPDEE method, can be promising techniques for the efficient and selective extraction of RG-I-enriched TBP.Keywords:Tartary buckwheat;deep eutectic solvent;high-pressure processing;rhamnogalacturonan-I-enriched pectic polysaccharide;biological activity
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JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Xinrong Li,Shuliang Chen,Xianzhi Hu,Futing Zi
Thiosulfate as a green and environmentally friendly lixiviant has received great attention in the field of gold extraction. However, the problem of gold ion recovery in thiosulfate gold leaching solution has hindered the large-scale application of this technology. Here, tri-n-butylphosphine (TBUP) was used as a functional monomer to prepare an adsorbent (CTS-TBUP) with abundant pores through the ion crosslinking reaction of chitosan, which was used to recover Au(I) from Au[(S 2 O 3 ) 2 ] 3− solution with high efficiency and large capacity. CTS-TBUP exhibited good potential for industrial applications with a gold loading capacity of 46.34 mg/g. CTS-TBUP exhibited a similar gold recovery performance for all pH ranges from 6 to 11, and the adsorption process of Au(I) on CTS-TBUP was well described by pseudo-second-order kinetics and Langmuir isothermal model. The mechanism by which CTS-TBUP adsorbs gold ions involves ligand exchange between TBUP and Au[(S 2 O 3 ) 2 ] 3− . In addition, Au(I) on CTS-TBUP will be reduced to elemental gold by TBUP in CTS-TBUP. The mechanism of Au(I) adsorption by CTS-TBUP was also confirmed by density functional theory calculations, and confirmed the rationality of forming a complex with a coordination ratio of 2:1 between TBUP and Au(I) at the theoretical level. CTS-TBUP has a good affinity for gold ions in thiosulfate solution, which provides a new idea for the preparation of gold adsorbent.
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DRUGS IN R&D
·
2024
·
IF 2.2
Jia Caiyun,Zhao Na,Song Haojing,Hu Yiting,Xu Yufang,Guo Caihui,Bai Wanjun,Dong Zhanjun
Background Ondansetron is a highly selective 5-HT3 receptor antagonist that alleviates nausea and vomiting. Bioequivalence evaluation ensures that the efficacy of generic drugs is consistent with that of the original drug. Objective The objective of this study was to evaluate the bioequivalence of ondansetron hydrochloride (HCl) tablets taken in single doses under fasting and postprandial conditions in healthy subjects. Methods In this randomized, open-label, two-cycle, crossover phase I study, liquid chromatography‒tandem mass spectrometry (LC‒MS/MS) was used to determine the ondansetron concentration in dipotassium-ethylenediaminetetraacetate (K 2 -EDTA) plasma after the subjects received a single 8 mg of ondansetron and reference formulation. Twenty-six healthy subjects received one tablet of ondansetron under fasting conditions and 28 subjects received one under postprandial conditions. Bioequivalence was established if the 90% confidence interval (CI) was 80.00–125.00%. The pharmacokinetic parameters were calculated via WinNonLin 8.1 software and the bioequivalence data were evaluated via Phoenix WinNonlin 8.1 statistics software. Results The geometric mean ratio (GMR) of the maximum observed concentration ( C max ), the area under the plasma concentration‒time curve (AUC) from time zero to the last sampling time (AUC 0– t ), and the AUC from time zero to infinity (AUC 0–∞ ) from the test/reference formulation under fasting conditions were 90.50, 90.43, and 90.25, respectively. The 90% CIs were 83.75–97.79, 82.64–98.95, and 82.25–99.03, respectively. The GMRs of C max , AUC 0– t , and AUC 0–∞ after a high-fat meal were 96.85, 93.57, and 93.77, respectively; the 90% CIs were 88.43–106.07, 87.35–100.24, and 87.35–100.68, respectively. Conclusion The test and reference formulations of ondansetron HCl have bioequivalence for healthy adult subjects under fasting and postprandial conditions.
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Analytical Methods
·
2024
·Vol. 16
·期 11
·1570-1578
·
IF 2.7
Meijuan Du,Xin Cheng,Qian Chen,Xueqin Xu
In this paper, a novel and sensitive electrochemical aptasensor for sulfadimethoxine (SDM) detection has been designed based on the triple helix structure/exonuclease I (Exo I)-assisted double signal amplification strategy. The aptamer probe (Apt) hybridizes with the signal transduction probe (STP) on the electrode to form a rigid double-stranded DNA (dsDNA) structure, so that the STP remains upright and methylene blue (MB) on the STP is far away from the electrode surface, resulting in a delicate current signal. In the presence of SDM, the SDM and Apt combine into a complex, leading to the transfer of the Apt and the exposure of the STP. Meanwhile, the added Exo I can digest the Apt to realize the cyclic amplification of SDM. After the addition of the signal probe (SP), a triple helix structure between the SP and STP is formed under acidic conditions, and MB on the STP and SP collide with the electrode surface to generate a strong electrochemical signal. The proposed aptasensor combines the features of the triple helix structure and Exo I to achieve double signal amplification for the sensitive detection of SDM with a wide linear range of 0.05–1000 ng mL−1 and a low detection limit of 0.02 ng mL−1. Furthermore, it has been successfully used to detect SDM in milk and lake water samples.
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