Analytical Cellular Pathology
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2021
·
IF 2.916
Teng Zhang,Xiaomin Chen,Yueqing Qu,Yanbing Ding
Oxidative stress-induced neuronal damage is a main cause of ischemia/reperfusion injury. Curcumin (Cur), the principal constituent extracted from dried rhizomes of Curcuma longa L. (turmeric), exhibits excellent antioxidant effects. Previous studies have indicated that miR-1287-5p was downregulated in patients with ischemic stroke. Additionally, we predicted that Lon Peptidase 2, Peroxisomal (LONP2), which is involved in oxidative stress regulation, is targeted by miR-1287-5p. The aim of the current study is to investigate the effect of Cur on ischemia/reperfusion damage and its underlying mechanism. To mimic ischemia/reperfusion damage environment, SH-SY5Y cells were subjected to oxygen-glucose-deprivation/reperfusion (OGD/R). OGD/R treatment downregulated miR-1287-5p and upregulated LONP2 in SH-SY5Y cells, but Cur alleviated OGD/R-induced oxidative damage and reversed the effect of OGD/R on the expression of miR-1287-5p and LONP2. Furthermore, we confirmed the interactive relationship between miR-1287-5p and LONP2 (negative regulation). We revealed that miR-1287-5p overexpression alleviated OGD/R-induced oxidative damage alleviation, similar to the effect of Cur. MiR-1287-5p inhibition accentuated OGD/R-induced oxidative damage in SH-SY5Y cells, which was reversed by Cur. The expression of LONP2 in OGD/R-treated SH-SY5Y cells was decreased by miR-1287-5p overexpression and increased by miR-1287-5p inhibition, and Cur counteracted the increase in LONP2 expression induced by miR-1287-5p inhibition. In conclusion, we suggest that Cur alleviates OGD/R-induced oxidative damage in SH-SY5Y cells by regulating the miR-1287-5p/LONP2 axis. The findings provide a theoretical basis for the clinical application of curcumin.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Yan Hu,Shangwen Zhang,Zhijie Wen,Hudie Fu,Jie Hu,Xuexin Ye,Li Kang,Xiaojun Li,Xinzhou Yang
Anti-inflammatory drugs for ulcerative colitis (UC) treatment should specifically penetrate and accumulate in the colon tissue. Herein, a multi-bioresponsive anti-inflammatory drug (curcumin, CUR)-loaded heterogeneous double-membrane microgels ( [email protected] ) for oral administration was fabricated in this study, in which the inner core was derived from polyvinyl alcohol (PVA) and guar gum (GG) and the outer gel was decoration with alginate and chitosan by polyelectrolyte interactions. The structure and morphology of microgels were characterized. In vitro, the formulation exhibited good bio-responses at different pH conditions and sustained-release properties in simulated colon fluid with a drug-release rate of 84.6 % over 34 h. With the assistance of the outlayer gels, the microgels effectively delayed the premature drug release of CUR in the upper gastrointestinal tract. In vivo studies revealed that [email protected] specifically accumulated in the colon tissue for 24 h, which suggest that the interlayer gels were apt to reach colon lesion. As expected, the oral administration of microgels remarkably alleviated the symptoms of UC and protected the colon tissue in DSS-induced UC mice. The above results indicated that these facilely fabricated microgels which exhibited excellent biocompatibility and multi-bioresponsive drug release, had an apparent effect on the treatment of UC, which represents a promising drug delivery strategy for CUR in a clinical application.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Yuelong Xu,Yu Zhang,Jingcheng Ma,Ting Wang,Chong Zhang
This study elucidates the mechanism of curcumin encapsulation in soy protein isolate (SPI) modified via enzymatic deamidation and pH-shifting. A novel Chryseobacterium sediminis -derived glutaminase (CS-PG) demonstrated superior thermal stability, catalytic efficiency, and specificity over commercial CP-PG. Controlled deamidation by both enzymes enhanced SPI functionality (solubility/emulsification/foaming) with minimal hydrolysis, where CS-PG induced hydrophobic self-assembly and CP-PG promoted hydrophilic domain formation. These distinct conformations dictated binding modes: CS-PG-SPI achieved 92.97 % higher encapsulation efficiency versus SPI through hydrophobic encapsulation, while attained an 85.00 % increase via surface-layer stabilization. pH-shifting further altered SPI conformation by inducing reversible unfolding and refolding, enabling formation of amorphous, stable protein–curcumin complexes. Dual modification significantly enhanced bioaccessibility versus free curcumin—1034 % for CS-SPI–Cur–pH and 1058 % for CP-SPI–Cur–pH. The work establishes enzymatic deamidation as a precision tool for tailoring SPI self-assembly and bioactive delivery in functional foods.
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MATERIALS & DESIGN
·
2025
·
IF 7.9
Peng Luo,Jiaming Fu,Xiaowei Yang,Fengbo Mo,Yanlong Zhong,Qi Lai,Shaorong Huang,Xiaoyong Zhang,Bin Zhang,Yen Wei
Osteoporosis is one of the most common chronic metabolic diseases that mainly caused by an imbalance in bone homeostasis. Considered its severe concurrent disorders, osteoporosis has become a global health issue, however, the anti-osteoporosis medications available in clinical practice are confronted with challenges such as high costs, adverse side effects, and limited efficacy. Herein, we reported construction of pH-responsive and bone-targeting nanomedicine (termed HPGCA) via click conjugation of alkyne functionalized hyperbranched polyglycerol (HPG) with curcumin (Cur) and alendronate (ALN) for the first time. We demonstrated that HPGCA exhibit superior water dispersibility, small particle size, and pH-dependent drug release behavior. Results from biological assays indicated that HPGCA displayed desirable bone-targeting capability, strong anti-osteoporosis efficacy, good hemocompatibility, and negligible adverse effects. More importantly, HPGCA showed better anti-osteoporosis efficacy than both free Cur and ALN according results from micro-CT and TRAP staining. The synergistic osteoporosis therapeutic efficacy would be explained by targeting delivering HPGCA to bone tissues and in-situ releasing cargos under acidic environment. Therefore, considered the above aspects, we believe that the innovative nanomedicine is promising for osteoporosis therapy in clinic and will provide new insights for full realization anti-osteoporotic properties of natural compounds
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2022
·
IF 5.895
Ya-Long Zheng,Zhi-Shan Tu,Hong-Mei Cui,Shuai Yan,De-Chen Duan,Wei Tang,Fang Dai,Bo Zhou
Reprograming of energy metabolism is a major hallmark of cancer, but its effective intervention is still a challenging task due to metabolic heterogeneity and plasticity of cancer cells. Herein, we report a general redox-based strategy for meeting the challenge. The strategy was exemplified by a dietary curcumin analogue (MitoCur-1) that was designed to target mitochondria (MitoCur-1). By virtue of its electrophilic and mitochondrial-targeting properties, MitoCur-1 generated reactive oxygen species (ROS) more effectively and selectively in HepG2 cells than in L02 cells via the inhibition of mitochondrial antioxidative thioredoxin reductase 2 (TrxR2). The ROS generation preferentially mediated the energy crisis of HepG2 cells in a dual-inhibition fashion against both mitochondrial and glycolytic metabolisms, which could hit the metabolic plasticity of HepG2 cells. The ROS-dependent energy crisis also allowed its preferential killing of HepG2 cells (IC50 = 1.4 μM) over L02 cells (IC50 = 9.1 μM), via induction of cell-cycle arrest, apoptosis and autophagic death, and its high antitumor efficacy in vivo, in nude mice bearing HepG2 tumors (15 mg/kg). These results highlight that inhibiting mitochondrial TrxR2 to produce ROS by electrophiles is a promising redox-based strategy for the effective intervention of cancer cell energy metabolic reprograming.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Zijun Wang,Huan Li,Shikai Zhang,Ke Ding,Ying Xie,Rongrong Wang,Liang Zhao,Wenjiang He,Yang Shan,Shenghua Ding
Plant proteins are increasingly used to encapsulate hydrophobic nutrients to formulate functional foods. A major challenge for plant protein-based nanocarriers is to improve the solubility and stability, thereby enhancing the absorption efficiency and bioavailability of hydrophobic nutrients within them. To address this issue, a free radical grafting strategy of folic acid (FA) modified pea protein isolate (PPI) was developed. The FA loading capacity of PPI-FA conjugates was 2.1-fold higher than that of PPI-FA mixtures. Conjugation of FA improved the solubility, thermal stability and storage stability of PPI. The photostability of FA was also enhanced (retention rate increased from 75.76 % to 94.92 %). Complexation with PPI-FA conjugate elevated the solubility, anti-cancer activity and cellular uptake of curcumin (Cur). The bioavailability of PPI-FA-Cur was 63.27 %, far exceeding those of free Cur (6.95 %) and PPI-Cur (24.47 %). These findings expand PPI's application and provide new reference for the development of advanced food delivery systems.
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Food Bioscience
·
2026
·
IF 6.2
Limai Tang,Xiaoqun Zeng,Yangying Sun,Hui Yu,Yangyang Hu,Daodong Pan
PPI-curcumin (Cur) interfacial complexation enables oil-based oleogel performance. • Interfacial complexation via Cur enhances oxidative stability in sausages. • Oil type dictates curcumin's efficacy in balancing sausage's nutrition and flavor. • Novel interfacial strategy for designing healthy fat replacer with improved quality. Developing plant protein-based oleogels with improved oxidative stability while maintaining desirable flavor remains a major challenge for healthy animal fat replacement. Herein, curcumin (Cur)-mediated interfacial complexation was employed to construct peanut protein isolate (PPI) oleogels using sunflower, flaxseed and corn oils. Curcumin strengthened the PPI interfacial film to form denser oleogel networks, improving emulsion stability and fat-replacement performance. Rheological measurements revealed that curcumin modulated the storage modulus and gel strength of oleogels contingent on oil matrix types. Microstructural observations further verified distinct aggregate and network-packing differences among three oil-based systems. Oleogels with curcumin complexation significantly reduced TBARS (by 69% in flaxseed oil and 31% in sunflower oil), while increasing immobilized water content (T 21 to 90.48% and 90.55% in sunflower and corn oils) and favorably modifying the relative fatty acid profile. Notably, elevated protein carbonyl contents were detected in flaxseed and corn oil based oleogel systems. Flax + Cur possessed highest relative PUFA proportion, whereas Sun + Cur and Corn + Cur exhibited preferable volatile profiles and higher sensory acceptability. Collectively, curcumin mediated interfacial complexation effectively regulated the structure-function relationship of PPI oleogels in an oil dependent manner, providing a promising interfacial engineering strategy for designing nutritionally enhanced animal fat replacers with improved flavor and oxidative stability. Download: Download high-res image (499KB) Download: Download full-size image
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CARBOHYDRATE POLYMERS
·
2026
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IF 13.2
Jing-jing Fu,Si-yi Mei,Yong-qiang Chen,Xiang-yu Fu,Yong-jie Long,Jun-xin Wu,Wen-qiang Cai,Yue-wen Chen
Polysaccharide-based hydrogel microspheres have demonstrated considerable potential for functional factors intestinal-targeted delivery and ulcerative colitis (UC) treatment. However, single-polysaccharide systems often struggle to achieve both stable loading and controlled release of functional factors. In this study, protein-polysaccharide composite microspheres were developed using a dual-crosslinking strategy. Initially, curcumin (Cur) was encapsulated within Andrias davidianus collagen (ADC) or its xylose glycated form (ADCXy) to form ADC/ADCXy-Cur complexes. These complexes were then crosslinked with sodium alginate (SA) and chelated with Ca 2+ ions to construct a primary hydrogel network (ADC/ADCXy-Cur-SA). Finally, the cationic polysaccharide chitosan (CS) with colon enzymolysis targeting, mucosal adhesion and pH responsive controlled release characteristics was incorporated with ADC/ADCXy-Cur-SA to form a double cross-linked multifunctional hydrogel microsphere (ADC/ADCXy-Cur-SA-CS). Compared with ADC-Cur-SA-CS, the ADCXy-Cur-SA-CS exhibited enhanced cellular antioxidant and anti-inflammatory activity, maintained high stability in simulated gastric conditions, and enabled controlled Cur release in colonic pH environment. In vivo experiments have shown that ADCXy-Cur-SA-CS alleviated UC based on the “one stone, four birds” strategy of antioxidant, anti-inflammatory, repairing the colon mucosal barrier, and regulating the gut microbiota. This study provides an important technical and theoretical foundation for the development of multifunctional polysaccharide-based hydrogel delivery systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
·
IF 8.7
Xi Wang,Yuying Wang,Ziqi Li,Bin Zou,Li Lin,Zhifeng Xue,Ruzhi Zhang,Weimin Wan
Hepatocellular carcinoma (HCC) remains a leading cause of cancer-related mortality worldwide due to late diagnosis, high metastasis, and poor therapeutic outcomes. Curcumin (Cur), a natural polyphenol with potent antitumor activity, is severely limited in clinical application by poor water solubility, low bioavailability, and insufficient tumor-targeting capability. Herein, a dual-targeted pH-responsive nanocarrier based on cellulose nanocrystals (CNCs) co-functionalized with folic acid (FA) and 3-carboxyphenylboronic acid (CA) was fabricated for enhanced Cur delivery to HCC cells. CNCs were aminated via silane coupling, followed by covalent co-grafting of FA and CA to obtain ACNCs@FA- CA. FA enabled targeting of folate receptors overexpressed on HCC cells, while CA played a dual role by reversibly binding Cur through boronate ester formation and recognizing sialic acid-rich tumor cell membranes. Cur was loaded with an efficiency of 13.90 ± 2.00% and exhibited pH-responsive release, reaching 81.61 ± 1.60% at pH 5.4 and 26.29 ± 1.47% at pH 7.2. In vitro studies demonstrated that Cur-loaded ACNCs@FA-CA significantly enhanced intracellular drug accumulation and cytotoxicity in HepG2 cells, with improved growth inhibition in a 3D hepatoma spheroid model. Collectively, this dual-targeted pH-responsive nanoplatform offers a promising strategy to improve the anti-HCC efficacy of Cur.
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Antioxidants
·
2026
·Vol. 15
·期 10
·1291
·
IF 8.2
Songlin Luo,Xudong Yang,Shunqiang Wei,Chushun Yi,Fansheng Zeng,Fali Zhang,Wei Zhang,Xiuli Chen,Yongzhen Zhao,Yinghui Wang,Aiguo Huang
White Spot Syndrome Virus (WSSV) remains a major factor limiting the sustainable development of crustacean aquaculture, creating an urgent need to develop comprehensive antiviral strategies with multiple protective functions. Natural bioactive compounds with antiviral, immunomodulatory, and antioxidant activities hold great potential for application; however, their poor stability and low bioavailability limit their practical use. In this study, self-assembled nanosystem (CE SA) composed of epigallocatechin gallate (EGCG) and curcumin (Cur) was constructed based on noncovalent interactions, and its protective effects against WSSV-infectedProcambarus clarkiiwere systematically evaluated. The results indicate that CE SA exhibits good stability, sustained-release properties, and biosafety. Compared with Cur, EGCG, and their physical mixture (CE), CE SA significantly improved the survival rate of infected shrimp and reduced viral copy numbers as well as the expression levels of key viral genes such asIE1,DNApol, andVP28. At the same time, CE SA significantly promoted the expression of relevant genes such asDorsal,Relish,SOD, andCAT, increased SOD and CAT enzyme activities and hematopoietic cell viability, and effectively alleviated damage to the hepatopancreas and intestinal tissues. Furthermore, metabolomic analysis indicated that CE SA regulates key metabolic processes such as amino acid metabolism, inflammation-related metabolism, nucleotide metabolism, and redox homeostasis, further elucidating its potential mechanism for enhancing the host’s antiviral capacity. In summary, by enhancing the stability and utilization efficiency of natural bioactive components, CE SA achieves superior anti-WSSV efficacy compared to individual components. Its protective effects are closely related to the inhibition of viral replication, enhancement of the immune response, improvement of antioxidant defenses, and regulation of host metabolic homeostasis. This study provides a promising, self-assembling nanostructure strategy for the green prevention and control of viral diseases in aquatic animals.
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FOOD HYDROCOLLOIDS
·
2026
·
IF 13.8
Fengqiong Jiang,Chaoran Fan,Mingfei Dang,Fuxiang Wei,Lu Liang,Lihua Fu,Chuanhui Xu,Baofeng Lin
A stable food-grade amphiphilic Lys/Alg nanoparticle emulsifier was prepared by double crosslinking. • An Edible, all-biomass-derived multifunctional Pickering emulsion was successfully developed via simple emulsification. • The Lys/Alg served as effective Pickering stabilizer enhanced the stability and bioavailability of curcumin. • The developed emulsion exhibited high antioxidant properties (99.02%) and broad-spectrum antibacterial activity. • The shelf life of various fruits was effectively extended by the emulsion coating. The integration of nano-encapsulation with edible coatings holds great potential for active fruit preservation. However, conventional emulsions often rely on chemical surfactants, posing safety risks and limited functionality. Here, we developed an edible, all-biomass-derived Pickering emulsion (CLAPE) coating stabilized by amphiphilic lysozyme/alginate composite nanoparticles (Lys/Alg). The Lys/Alg were fabricated through electrostatic self-assembly followed by Ca 2+ cross-linking, forming a robust interfacial layer that effectively encapsulates curcumin (Cur). Molecular docking simulations revealed a strong binding affinity between Lys and Cur (binding energy: −87.2 kcal/mol), which significantly enhances the aqueous dispersibility and stability of Cur. The resulting Pickering emulsion enables sustained release of curcumin over 60 h, with a peak cumulative release of 94.0%, approximately twice that of the unencapsulated control. This release profile follows Fickian diffusion, extending the bioactive duration and increasing the total released amount, thereby enhancing curcumin's bioavailability. Moreover, CLAPE exhibits excellent thermal stability, maintaining high stability indices under varied accelerated storage conditions. It also exhibited high antioxidant (99.02%) and broad-spectrum antibacterial activity. Additionally, the application of CLAPE on strawberries, bananas and grapes has led to a doubling of their shelf life while maintaining the freshness and nutrient content of the fruits. The edible active preservative material, based entirely on biomass, not only forms a multifunctional protective coating that prevents spoilage but can also be easily washed off. This study presents an innovative strategy for the designs of active preservation materials utilizing all-natural plant-derived components. Download: Download high-res image (495KB) Download: Download full-size image
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Dongdong Guo,Wenlida Mu,Chang Liu,He Qian
Three colloidal systems (LP-CP, SC-CP, CYC-CP) were systematically compared for encapsulating curcumin- Brassica rapa L. polysaccharide mixture (CP). • SC-CP exhibited the highest curcumin loading capacity, superior curcumin thermal stability, and peak colonic accumulation of curcumin in vivo . • SC-CP significantly promoted the production of acetate, propionate, and butyrate during in vitro colonic fermentation, outperforming LP-CP and CYC-CP. • In a mouse model, SC-CP enriched beneficial genera, including Lachnospiraceae_NK4A136_group and Akkermansia and increased cecal butyrate levels. • The choice of colloidal carrier fundamentally governs the colonic delivery efficiency and gut microbiota remodeling capacity of the curcumin-polysaccharide mixture. The therapeutic potential of curcumin is severely constrained by its poor physicochemical stability and low oral bioavailability. Co-formulation with Brassica rapa L. polysaccharide offers a promising strategy to enhance its functionality; however, the impact of different colloidal delivery systems on the encapsulation performance and subsequent biological fate of this mixture remains unclear. In this study, we systematically compared three spray-dried delivery platforms, including liposomes (LP-CP), sodium caseinate nanoparticles (SC-CP), and β-cyclodextrin inclusion complexes (CYC-CP), for encapsulating CP. Our results demonstrated that the carrier system critically determined encapsulation performance, with SC-CP exhibiting superior curcumin loading capacity (7.24%), curcumin thermal stability (82.87% retention at 95 °C), and favorable hygroscopicity profiles. Notably, SC-CP facilitated enhanced colonic accumulation in vivo , achieving a peak accumulation of 53.45% at 8 h post-gavage, representing a 15-fold increase compared to curcumin from unencapsulated CP. By integrating in vitro fermentation models with in vivo animal experiments and employing 16S rRNA sequencing alongside short-chain fatty acid (SCFA) analysis, we systematically elucidated the carrier-specific modulatory effects on the gut microbiota. In the in vitro fermentation system, SC-CP significantly promoted the production of acetate, propionate, and butyrate, while enriching butyrate-producing genera such as Lachnospiraceae_NK4A136_group . In the in vivo animal model, SC-CP intervention resulted in a 1.6-fold increase in cecal butyrate levels and a marked increase in the abundance of beneficial genera, including Akkermansia , demonstrating superior modulation of microbial community structure and metabolic function. Collectively, these findings establish that the selection of a colloidal carrier not only dictates the physicochemical stability of the curcumin-polysaccharide mixture but also governs its spatiotemporal distribution within the gastrointestinal tract and its prebiotic capacity to remodel the gut microecology. This study provides a theoretical foundation for the rational selection of delivery systems to maximize the functional efficacy of bioactive ingredients in functional food applications. Download: Download high-res image (195KB) Download: Download full-size image
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