CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Jun Wang,Leyna G. Liu,Wen-Qiao Jiao,Hua Yang,Jing Liu,Dahai Liu
In order to achieve high loading and efficient delivery of curcumin, phenylboronic acid-conjugated chitosan nanoparticles were prepared by a simple desolvation method. These nanoparticles exhibited a regular spherical shape with the average size about 200−230 nm and narrow size distribution, which were kinetically stable under physiological condition. Due to boronate ester formation between curcumin and phenylboronic acid groups in the nanoparticles, and the hydrogen bonding interactions between curcumin and nanocarriers, curcumin was successfully loaded into the nanoparticles with high drug loading content. These curcumin-loaded nanoparticles showed pH and reactive oxygen species (ROS)-triggered drug release behavior. In vitro cell experiments revealed that the blank nanoparticles were completely nontoxic to cultured cells, and the curcumin-loaded nanoparticles exhibited efficient antitumor efficiency against cancer cells. Moreover, the drug-loaded nanoparticles performed an enhanced growth inhibition in three-dimensional multicellular tumor spheroids. Thus, these nanocarriers would be a promising candidate for curcumin delivery in tumor treatment.
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CARBOHYDRATE POLYMERS
·
2020
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IF 7.182
Xiao Ma,Guomin Wu,Fangfang Dai,Dan Li,Hao Li,Li Zhang,Hongbing Deng
Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility. However, the practical applications of pure SF biomaterials confront bacterial infection problems. In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility. The surface morphology and composition analysis confirmed the successful deposition. After depositing 15 bilayers , the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa. A trend towards better hydrophilicity performance was also recorded with more bilayers coating on the mats. Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus . In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells. Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
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IONICS
·
2020
·Vol. 26
·期 12
·6371-6378
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IF 2.394
Xu Haoyan,Han Chuanlong,Liu Sicheng,Hao Xin,Rao Yu,Gong Zexin,Sun Zhuangzhi
Due to the efficiency of ion movement is greatly affected by moisture environment, this article explores the effects of the changing the moisture content of the self-degraded ionic hydrogel-based electrolyte layer made of sodium alginate and chitosan on the improvement of the electromechanical properties of the soft ionic actuator. Specifically, sodium alginate-chitosan ionic hydrogel-based soft ionic actuator is fabricated and analyzed by voltammetry analysis, AC impedance, charge and discharge, electrical conductivity, displacement, and output force. Furthermore, the further application of this soft ionic actuator on bionic flytrap is developed and discussed. The results show that soft ionic actuator with the moisture content of 78.64% has the best electromechanical properties. Furthermore, soft ionic actuators with different moisture contents show different degrees of deflection due to the shift of the anions to the cathode under charged conditions. Therefore, it can be found that moisture content is beneficial to improve the electromechanical properties of ionic hydrogel-based soft artificial muscle.
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CARBOHYDRATE POLYMERS
·
2020
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IF 7.182
Xiaoyu Zhang,Huabing Yang,Junping Zheng,Nan Jiang,Guangjun Sun,Xinkun Bao,Aizhen Lin,Hongtao Liu
This study was designed to explore the improvement of chitosan oligosaccharides (COS) on constipation through regulation of gut microbiota. Here, we proved that COS treatment profoundly boosted intestinal motility, restrained inflammatory responses, improved water-electrolyte metabolism and prevented gut barrier damage in constipated mice induced by loperamide . By 16S rDNA gene sequencing, the disbalanced gut microbiota was observed in constipated mice, while COS treatment statistically reversed the abundance changes of several intestinal bacteria at either phylum, family and genus levels, which partly led to the balance in production of intestinal metabolites including bile acids, short-chain fatty acids and tryptophan catabolites. In addition, COS failed to relieve the constipation in mice with intestinal flora depletion, confirming the essentiality of gut microbiota in COS-initiated prevention against constipation. In summary, COS can ameliorate the development of loperamide-induced constipation in mice by remodeling the structure of gut microbial community.
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Materials Science & Engineering C-Materials for Biological Applications
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2021
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IF 7.328
Yang Yang,Alastair Campbell Ritchie,Nicola M. Everitt
Animal-derived collagen may contain viruses, and its impurity can cause immunological reactions. Chitosan, always required a neutralization step in fabricating it into the biocompatible tissue engineering scaffolds. To avoid these risks and simplify the production process, a series of recombinant human collagen/carboxylated chitosan (RHC-CHI) based soft hydrogel scaffolds were prepared by crosslinking-induced gelation and then investigated their feasibilities for use as soft tissue engineering scaffolds. The gelation time was optimized by modulating the biopolymer concentration or reaction temperature. The hydrogel swelling, degradation rate, and mechanical properties were also investigated. The results showed that these parameters could be tuned by adjusting either the RHC-to-chitosan ratio or the total polymer concentration. The mechanical properties of the hydrogels were improved by adding chitosan, but excess chitosan reduced the hydrogel mechanical strength and accelerated the degradation speed. Cytotoxicity tests showed that all fabricated soft hydrogels were biocompatible and displayed no cytotoxicity. Cytocompatibility tests and qRT-PCR studies indicated that the hydrogel system promoted the adhesion and proliferation of NIH-3T3 cells, and cellular activities were directly up-regulated by RHC. Finally, our in vivo study proved these hydrogels were able to accelerate the cell infiltration and wound closure. These results show that the soft RHC-CHI hydrogels show promise in soft-tissue engineering.
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Polymers
·
2020
·Vol. 12
·期 4
·916
·
IF 3.426
Mengjie Wang,Yonggang Shangguan,Qiang Zheng
In this work, the transformation of chitosan-grafted-polyacrylamide (GPAM) aggregates in aqueous solution upon heating was explored by cryo-electron microscope (cryo-TEM) and dynamic light scattering (DLS), and larger aggregates were formed in GPAM aqueous solution upon heating, which were responsible for the thermo-thickening behavior of GPAM aqueous solution during the heating process. The heating initiates a transformation from H-bonding aggregates to a large-sized cluster formed by self-assembled hydrophobic chitosan backbones. The acetic acid (HAc) concentration has a significant effect on the thermo-thickening behavior of GPAM aqueous solution; there is a critical value of the concentration (>0.005 M) for the thermo-thickening of 10 mg/mL GPAM solution. The concentration of HAc will affect the protonation degree of GPAM, and affect the strength of the electrostatic repulsion between GPAM molecular segments, which will have a significant effect on the state of the aggregates in solution. Other factors that have an influence on the thermo-thickening behavior of GPAM aqueous solution upon heating were investigated and discussed in detail, including the heating rate and shear rate.Keywords:chitosan-grafted-polyacrylamide;thermo-thickening;rheological;dynamic light scattering;cryo-electron microscope
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Theranostics
·
2020
·
IF 8.579
Yuping Hong,Yaguang Han,Jun Wu,Xinxin Zhao,Jin Cheng,Guo Gao,Qirong Qian,Xiuying Wang,Weidong Cai,Hala Zreiqat,Dagan Feng,Jianrong Xu,Daxiang Cui
Chondral and osteochondral defects caused by trauma or pathological changes, commonly progress into total joint degradation, even resulting in disability. The cartilage restoration is a great challenge because of its avascularity and limited proliferative ability. Additionally, precise diagnosis using non-invasive detection techniques is challenging, which increases problems associated with chondral disease treatment. Methods: To achieve a theranostic goal, we used an integrated strategy that relies on exploiting a multifunctional nanoprobe based on chitosan-modified Fe3O4 nanoparticles, which spontaneously self-assemble with the oppositely charged small molecule growth factor, kartogenin (KGN). This nanoprobe was used to obtain distinctively brighter T 2 -weighted magnetic resonance (MR) imaging, allowing its use as a positive contrast agent, and could be applied to obtain accurate diagnosis and osteochondral regeneration therapy. Results: This nanoprobe was first investigated using adipose tissue-derived stem cells (ADSCs), and was found to be a novel positive contrast agent that also plays a significant role in stimulating ADSCs differentiation into chondrocytes. This self-assembled probe was not only biocompatible both in vitro and in vivo , contributing to cellular internalization, but was also used to successfully make distinction of normal/damaged tissue in T 2 -weighted MR imaging. This novel combination was systematically shown to be biosafe via the decrement of apparent MR signals and elimination of ferroferric oxide over a 12-week regeneration period. Conclusion: Here, we established a novel method for osteochondral disease diagnosis and reconstruction. Using the Fe 3 O 4 -CS/KGN nanoprobe, it is easy to distinguish the defect position, and it could act as a tool for dynamic observation as well as a stem cell-based therapy for directionally chondral differentiation.
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CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Fengxia Wang,Xu Ding,Xiaobo Niu,Xianyi Liu,Wei Wang,Ji Zhang
Although core-shell structure is favored by many applications, preparing it with green way is rarely been reported. Herein, a core-shell structured Cu@Pd-CS nanocomposite is greenly fabricated utilizing a natural chitosan and applied to glucose detection. As-obtained Cu@Pd-CS nanoparticles were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and powder X-ray diffraction (XRD). When applied to glucose detection, the Cu@Pd-CS exhibits good stability, sensitivity and anti-interference. Moreover, it has a good linear relationship in glucose concentrations range of 0.1−1 mM with the sensitivity of 1.53 μA mM −1 cm −2 and 1−10 mM with the sensitivity of 23.00 μA mM −1 cm −2 . This work proves the practicability of building metal-based core-shell structure nanoparticles with green resources and glucose detection application.
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高影响因子
Nanoscale Research Letters
·
2020
·Vol. 15
·期 1
·1-13
·
IF 3.578
Cai Defu,Han Cuiyan,Liu Chang,Ma Xiaoxing,Qian Jiayi,Zhou Jianwen,Li Yue,Sun Yiming,Zhang Changting,Zhu Wenquan
An enzyme-responsive colon-specific delivery system was developed based on hollow mesoporous silica spheres (HMSS) to which biodegradable chitosan (CS) was attached via cleavable azo bonds (HMSS–N=N–CS). Doxorubicin (DOX) was encapsulated in a noncrystalline state in the hollow cavity and mesopores of HMSS with the high loading amount of 35.2%. In vitro drug release proved that HMSS–N=N–CS/DOX performed enzyme-responsive drug release. The grafted CS could increase the biocompatibility and stability and reduce the protein adsorption on HMSS. Gastrointestinal mucosa irritation and cell cytotoxicity results indicated the good biocompatibility of HMSS and HMSS–N=N–CS. Cellular uptake results indicated that the uptake of DOX was obviously increased after HMSS–N=N–CS/DOX was preincubated with a colonic enzyme mixture. HMSS–N=N–CS/DOX incubated with colon enzymes showed increased cytotoxicity, and its IC 50 value was three times lower than that of HMSS–N=N–CS/DOX group without colon enzymes. The present work lays the foundation for subsequent research on mesoporous carriers for oral colon-specific drug delivery.
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CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Rui Chen,Zijin Luo,XueJun Yu,Hao Tang,Yuan Zhou,Hong Zhou
A novel flame retardant CCD was synthesized by the condensation between cinnamalde and chitosan, followed by the addition reaction with 9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide(DOPO), and CCD was named from the initials of the three raw materials. The intermediate product CC and the target product CCD was then characterized by infrared spectrometry and elemental analysis. The flame retardancy of EP thermosets modified by CCD was dramatically improved. Epoxy resin (EP) with 10wt% CCD passed vertical burning (UL-94) V-0 rating and possessed limited oxygen index (LOI) value of 31.6 %. Cone calorimeter test exhibited the introduction of 3.5g CCD into 35g EP decreased the total heat release by 38.8 % and decreased the total smoke product by 72.0 %. XPS, FTIR, SEM and Raman tests were proceeded to determine the char residue for EP/10 % CCD thermoset, and the results showed that char residue for EP/10 % CCD thermoset possessed dense and compact structure which played a positive effect in blocking the exchange of heat and gas.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Piao Cheng,Hongyi Gao,Xiao Chen,Yuanyuan Chen,Mengyi Han,Liwen Xing,Panpan Liu,Ge Wang
Currently, most reported composite phase change materials (PCMs) are powdery shape, which require secondary processing for practical applications. Although some monolithic composite PCMs have been developed, their flexibility usually undergoes a remarkable reduction or even complete disappearance when supporting material s are infiltrated with PCMs. To solve this problem, we fabricated a flexible supporting material with a folded layer-bridge network structure by dispersing carbon nanotubes (CNTs) in acetic acid solution of chitosan (CS) with poly(vinyl alcohol) (PVA) using a directional freezing method. Then CS/PVA/CNTs (CPC) scaffold was infiltrated with polyethylene glycol (PEG) to prepare PEG@CPC composite PCM. The resulting flexible composite PCM displays excellent mechanical properties, such as high tensile strength of 2.42 MPa and bending resistance of >100 cycles. Moreover, it displays outstanding thermal properties, such as high crystallinity of close to 100% and encapsulation ratio of 92.6 wt%. This work provides a simple method for preparation of flexible monolithic composite PCMs for many potential applications, such as wearable fitting-skin temperature-controlled materials.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Jincan He,Yuyuan Su,Zijun Sun,Runkun Zhang,Fuhai Wu,Yan Bai
In this paper, a fluorometric method based on chitosan-mediated “turn-on” strategy was proposed for rapid detection of perfluorooctane sulfonate (PFOS). The method can be performed by two steps of simple physical mix. The whole procedure takes no more than 25 min. In pH 5.50 Britton-Robinson buffer solution, a green fluorescent dye (trisodium-8-hydroxypyrene-1,3,6-trisulfonate, HPTS) reacted with protonated chitosan to form complex by electrostatic attraction, leading in the strong quenching of HPTS. In the presence of PFOS, the fluorescence of HPTS recovered resulting from the electrostatic and hydrophobic interactions between PFOS and chitosan. The spectral characteristics, the reaction mechanisms of fluorescence quenching and fluorescence recovery, and the analytical conditions were investigated. Under the optimum conditions, a fluorometric method based on the chitosan-mediated “turn-on” strategy was developed for detection of PFOS. The value of fluorescence restoration (Δ F ) was proportional to the concentration of PFOS in range of 0.0050–2.0 μmol/L with correlation coefficient of 0.9949. The limit of detection (LOD) was calculated to be 1.0 nmol/L. The method was applied to the determination of PFOS in environment water with recoveries of 90.8–102% and RSD of 2.8–5.9%. The fluorometric method based on chitosan-mediated “turn-on” strategy is simple, rapid, sensitive and economical, showing potentiality for detection of PFOS in environmental water samples.
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