SENSORS AND ACTUATORS B-CHEMICAL
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2020
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IF 7.1
Ying Liu,Shuai Yue,Ya-Ning Wang,Yue Wang,Zhang-Run Xu
A multicolor and surface-enhanced Raman scattering (SERS) dual-mode pH sensing method based on microparticles was proposed, which combined the advantages of high sensitivity of SERS and convenience of colorimetric visualization. Polyethylene glycol diacrylate microgels were fabricated using a T-channel microfluidic chip, and the polyaniline-coated gold nanorods were encapsulated in the microgels. Based on the nano-in-micro particles, pH monitoring within the range of 1.0–12.0 was achieved through multicolor and SERS dual-mode sensing. The protonation and deprotonation of polyaniline led to changes in Raman signal intensity and color of microparticles. The pH changes during cell culture were successfully monitored by SERS quantification and microscopic images of the microparticles. And the microparticles exhibit excellent stability, reversibility, biocompatibility, anti-interference ability and signal enhancement ability. The pH sensing method has a wide pH detection range and is especially suitable for pH monitoring in microsystems and micro-environments.
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CARBOHYDRATE POLYMERS
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2020
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IF 7.182
Qun Liu,Yan Li,Xiaodeng Yang,Shu Xing,Congde Qiao,Shoujuan Wang,Chunlin Xu,Tianduo Li
Chitosan has attracted much attention in drug delivery, however, carboxymethyl chitosan (CMC)-based self-aggregated nanocarriers are seldom reported. In this paper, two kinds of CMC-based pH-responsive amphiphilic chitosan derivatives, N -2-hydroxylpropyl-3-butyl ether- O -carboxymethyl chitosan (HBCC) and N -2-hydroxylpropyl-3-(2-ethylhexyl glycidyl ether)- O -carboxymethyl chitosan (H2ECC), have been synthesized by the homogeneous reaction. The molecular structures were characterized by FTIR, 1 H NMR and 13 C NMR. The optimum reaction condition was obtained based on the data of 1 H NMR spectrum: reaction time of 4 h, reaction temperature of 80 °C and n e p o x y n - N H 2 of 3/1, respectively. The XRD patterns showed the crystallinity of HBCC and H2ECC decreased due to the introduction of hydrophobic segments. The thermostability of HBCC and H2ECC was improved for the formation of heat-resistant stereo-complexed structures. The intermolecular hydrophobic interaction hindered the intermolecular mobility by increasing glass transition temperature of ca. 10 °C. Both HBCC and H2ECC have very low critical aggregation concentrations (HBCC: 0.66–1.56 g/L, H2ECC: 0.57–1.07 g/L) and moderate aggregate particle size, which is advantageous for utilization as a drug carrier. The curcumin loaded HBCC and H2ECC aggregates showed nontoxicity, meanwhile, HBCC and H2ECC showed good antibacterial activity against Staphylococcus aureus and Escherichia coli . As a result of these two favorable properties, HBCC and H2ECC could be used as curcumin nanocarriers as well as antibacterial agents.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2020
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IF 7.489
Lihui Wan,Zhongyin Chen,Yan Deng,Tao Liao,Ying Kuang,Jia Liu,Junlin Duan,Ziqiang Xu,Bingbing Jiang,Cao Li
The integration of diagnostic and therapeutic functions in a nanoplatform has been a rapidly emerging method in the management of cancer. The application of imaging technology paves the way to track the pharmacokinetics of the nanoplatforms, to guide the treatment, and to monitor the therapeutic processes and outcomes. Herein, we reported a novel type of monodisperses mesoporous silica-coated superparamagnetic iron oxide-based multifunctional nanoplatform (DOX@MMSN-SS-PEI-cit) for the diagnosis and treatment of cancer. The fabrication process included the surface modification of monodisperses mesoporous silica nanoparticle (MMSN) with branched polyethyleneimine (PEI) via disulfide bonds and the further coupling of citraconic anhydride to PEI . Typically, the hydrolysis of amide bonds in the tumor microenvironment (TME) could lead to a negative-to-positive charge reversion, which can enhance the endosomal escape of the resulting nanoplatform. The rapid release of doxorubicin hydrochloride (DOX) directly killed the cancer cells. Due to the superparamagnetic iron oxide-based high-resolution T 2 -weighted MR imaging contrast agents, this novel multifunctional nanoplatform successfully realized MR imaging, targeted drug delivery and controlled release in one system, and achieved significant improvement in tumor diagnosis and therapy. In summary, the therapeutic nanoplatform is a promising option in precise cancer treatment.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2020
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IF 3.616
Dongqin Lei,Quan Wang,Yue Kong,Yuanwei Chen,Xianglin Luo
It is important to reduce side effects and to explore novel usage for hydrophobic broad-spectrum antibacterial agent triclosan (TCS). In this study, a new amphiphilic copolymer with tertiary amine groups, monomethyl ether poly(ethylene glycol)-b-poly{α-[4-(diethylamino)methyl-1,2,3-triazol]-caprolactone-co-caprolactone} (mPEG-PDCL) was designed and synthesized, and its micelles were applied as carries of TCS to enhance antimicrobial and bacteriostatic action. mPEG-PDCL and its contrastive copolymer mPEG-PCL could form uniform spherical micelles with sizes 50-110 nm . The zeta potential of mPEG-PDCL micelles was positive and changed from 7.00 ± 0.67 mV at pH 7.5 to 24.67 ± 1.23 mV at pH 5.5. Both TCS-loaded micelles displayed quite high drug loading content (approx. 15%) and drug loading efficiency (more than 85%). In comparison with pH 7.4, TCS released faster in acidic environment which was induced by bacteria metabolism. MIC values of both TCS-loaded micelles against S. aureus and E. coli were as low as free TCS. TCS-loaded micelles showed much better antibacterial activity than free TCS, especially, mPEG-PDCL/TCS micelles displayed long bacteriostatic efficacy in 60 h against S. aureus and in 54 h against E. coli . mPEG-PDCL micelles preferred targeting to both S. aureus and E. coli due to positive zeta potential. In in vivo experiment, the purulence of the infected wound almost disappeared for SD rats treated with mPEG-PDCL/TCS micelles. Therefore, mPEG-PDCL micelles may be used as good carriers for antimicrobial agents, and the TCS-loaded micelles possess long antimicrobial/bacteriostatic efficacy.
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CHEMICAL PHYSICS
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2020
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IF 1.771
Zhizhuo Lu,Lihong Wang,Hujun Xie,Qunfang Lei,Wenjun Fang,Xiaoxing Lu
Colloid particles consisting of biopolymers such as proteins and polysaccharides have been widely used as food or drug components. Here we investigate the interactions and structures in aqueous mixtures of ovalbumin (OVA) and κ-carrageenan (CRG) to unravel the complexation mechanism. The pH titration results for the OVA/CRG system (0.025%:0.025% by mass) indicate the structural transition of the OVA/CRG complexes happens during acidification. Significant accumulation of insoluble OVA/CRG complex coacervates with OVA packed in the hydrophobic structures formed by CRG occurs when pH is lower than the isoelectric point of OVA. The highest turbidity is reached at pH = 3.0 because of the strongest electrostatic attraction, and further deceasing pH leads to the dissociation of complex coacervates. Composition and ionic strength show significant effects on turbidity and aggregates formation by tuning electrostatic interactions between N–H + of –NH 3 + on OVA and S–O − of –SO 3 − on CRG.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
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IF 4.389
Huaxin Zhang,Qinghua Xia,Dan Zhou
Silica nanoparticles as drug delivery systems (DDS) have received huge attention in nanomedicine research. However, their drug release processes were usually discussed in the absence of proteins, which are abundant in real delivery media and likely to affect the release behaviors. In this work, novel pH-sensitive silica-based DDSs were constructed using the endogenous protein, human serum albumin (HSA), as the gatekeeper, and their release properties in protein-containing media were measured for the first time. As-synthesized mesoporous silica nanorod (MSNR) was modified with zwitterions to suppress the nonspecific adsorption of protein and to improve the dispersity. HSA was fixed onto MSNR through drug-protein interactions and covalent bonds, respectively. Benzoic-imine bonds were introduced into the designs to realize pH responsiveness. The fluorescence quenching effect of drugs on HSA was used to monitor the drug release in simulated body fluids containing proteins. The results indicated that protein gating could effectively reduce premature release in normal blood environment. The endogenous protein capping, high dispersity and stability, considerable loading content, low cytotoxicity and favorable responsiveness to acidic microenvironment enable the present structures to be promising carriers for chemotherapy drugs.
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DESALINATION
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2020
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IF 7.098
Kai Zhang,Kang Yang,Yingbo Chen,Yunxia Hu
Ionic and pH responsive thin film composite (TFC) membrane is prepared by interfacial polymerization of lysine and trimesoyl chloride on the out surface of poly ( m -phenylene isophthalamide) hollow fiber membrane . Hydrophilicity and strong negative charge of the TFC membrane are obtained by introducing a large amount of carboxylic groups in the cross-linked polyamide network, endorsing the ionic and pH response of the TFC membrane in terms of flux and rejection. The TFC membrane shows almost 100% rejection for Eriochrome black T, with a flux of 35.8 L/m 2 h (0.2 MPa) at low pH and salt concentration . However, the rejection decreases to 95.8%, and the flux increases more than three times at high pH and salt concentration due to the shift of the interaction in the polyamide network from hydrogen bonding to static-electric repulsion. The responsive TFC membrane also shows great performance in separation of dye-salt mixture and dye mixture.
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高影响因子
MOLECULES
·
2020
·Vol. 25
·期 17
·3871
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IF 3.267
Ziyuan Wang,Jie Liu,Jian Gao,Mengna Cao,Gerui Ren,Hunjun Xie,Mingfei Yao
Protein–polysaccharide complexes have received increasing attention as delivery systems to improve the stability and bioavailability of multiple bioactive compounds. However, deep and comprehensive understanding of the interactions between proteins and polysaccharides is still required for enhancing their loading efficiency and facilitating targeted delivery. In this study, we fabricated a type of protein–polysaccharide complexes using food-grade materials of β-lactoglobulin (β-Lg) and gum arabic (GA). The formation and characteristics of β-Lg–GA complexes were investigated by determining the influence of pH and other factors on their turbidity, zeta-potential, particle size and rheology. Results demonstrated that the β-Lg and GA suspension experienced four regimes including co-soluble polymers, soluble complexes, insoluble complexes and co-soluble polymers when the pH ranged from 1.2 to 7 and that β-Lg–GA complexes formed in large quantities at pH 4.2. An increased ratio of β-Lg in the mixtures was found to promote the formation of β-Lg and GA complexes, and the optimal β-Lg/GA ratio was found to be 2:1. The electrostatic interactions between the NH3+group in β-Lg and the COO−group in GA were confirmed to be the main driving forces for the formation of β-Lg/GA complexes. The formed structure also resulted in enhanced thermal stability and viscosity. These findings provide critical implications for the application of β-lactoglobulin and gum arabic complexes in food research and industry.Keywords:protein–polysaccharide complexes;β-lactoglobulin;gum arabic;turbidity;electrostatic interactions
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Theranostics
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2020
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IF 8.579
Yuwei Li,Shuzhen Yue,Jingyu Cao,Chengzhan Zhu,Yixiu Wang,Xin Hai,Weiling Song,Sai Bi
Chemo-gene therapy is an emerging synergetic modality for the treatment of cancers. Herein, we developed pH-responsive multifunctional DNA nanomicelles (DNMs) as delivery vehicles for controllable release of doxorubicin (Dox) and anaplastic lymphoma kinase (ALK)-specific siRNA for the chemo-gene synergetic therapy of anaplastic large cell lymphoma (ALCL). Methods: DNMs were synthesized by performing in situ rolling circle amplification (RCA) on the amphiphilic primer-polylactide (PLA) micelles, followed by functionalization of pH-responsive triplex DNA via complementary base pairing. The anticancer drug Dox and ALK-specific siRNA were co-loaded to construct Dox/siRNA/DNMs for chemo-gene synergetic cancer therapy. When exposed to the acidic microenvironment (pH below 5.0), C-G·C + triplex structures were formed, leading to the release of Dox and siRNA for gene silencing to enhance the chemosensitivity in ALCL K299 cells. The chemo-gene synergetic anticancer effect of Dox/siRNA/DNMs on ALCL was evaluated in vitro and in vivo . Results: The pH-responsive DNMs exhibited good monodispersity at different pH values, good biocompatibility, high drug loading capacity, and excellent stability even in the human serum. With the simultaneous release of anticancer drug Dox and ALK-specific siRNA in response to pH in the tumor microenvironment, the Dox/siRNA/DNMs demonstrated significantly higher treatment efficacy for ALCL compared with chemotherapy alone, because the silencing of ALK gene expression mediated by siRNA increased the chemosensitivity of ALCL cells. From the pathological analysis of tumor tissue, the Dox/siRNA/DNMs exhibited the superiority in inhibiting tumor growth, low toxic side effects and good biosafety. Conclusion: DNMs co-loaded with Dox and ALK-specific siRNA exhibited significantly enhanced apoptosis of ALCL K299 cells in vitro and effectively inhibited tumor growth in vivo without obvious toxicity, providing a potential strategy in the development of nanomedicines for synergetic cancer therapy.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2020
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IF 2.734
Miaomiao Long,Shenhuan Liu,Xiaotian Shan,Jing Mao,Fuwei Yang,Xiaoyu Wu,Lipeng Qiu,Jinghua Chen
Polymeric micelles have attracted much attention in the field of anticancer drug delivery system because of their special properties. However, the slow drug release rate of micelles after reaching tumor cells is still considered to be a major problem. Therefore, we constructed a new orthoester-based pH-sensitive polyethylene glycol–hydroxydodecanoic acid (PAE) polymer to form doxorubicin-loaded micelles (DOX/PAE). DOX/PAE micelles with uniform particle size showed pH-sensitive drug release behavior in vitro . And the in vitro cytotoxicity studies showed that blank PAE micelles were nontoxic to cells while DOX/PAE micelles could inhibit the growth of tumor cells in a concentration-dependent manner. Furthermore, cellular uptake studies showed that DOX/PAE micelles were uptaken by tumor cells and transported into the nucleus to achieve the effect of killing tumor cells. The results of the uptake mechanism showed that clathrin and macrophage mediated endocytic pathways played important roles in internalization of DOX/PAE micelles. Altogether, the above results show that pH-sensitive PAE micelles have potential in anticancer drug delivery.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
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IF 4.192
Hui He,Xiaoyu Shi,Wenbo Chen,Rimei Chen,Chao Zhao,Shuangfei Wang
Novel nanosized biomass-based pH- and temperature-responsive cellulose nanofibers (TOCNF-HPEI-IBAm) were designed and prepared by grafting hyperbranched polyethylenimine (HPEI) modified with isobutyramide (IBAm) groups (HPEI-IBAm) onto carboxylated cellulose nanofibers (TOCNFs). The as-prepared TOCNF-HPEI-IBAm possessed excellent biocompatibility and pH- and temperature-responsive properties. TOCNF-HPEI-IBAm showed a rapid wettability conversion from hydrophilic (WCA = 41.1°, WCA = 70.7°) to hydrophobic (WCA = 147.3°, WCA = 142.2°) in response to changes in pH and temperature from acidic conditions to alkaline conditions and from lower to higher temperatures. In addition, it possesses strong antibacterial activity against Escherichia coli and Listeria (Eb ≥ 97%). The amount of DOX loaded in TOCNF-HPEI-IBAm was 642.52 mg/g, and the maximum amount of DOX released was 39.30% at pH = 3.0 within 9 h. Furthermore, the dual interactions stimulus-responsive mechanism was revealed to be attributed to the expansion and collapse of the molecular chains of TOCNF-HPEI-IBAm in response to temperature and pH through mutual promotion and inhibition.
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CHEMBIOCHEM
·
2020
·Vol. 21
·期 18
·2620-2627
·
IF 2.576
Dandan Li,Qianqian Guo,Liming Ding,Wei Zhang,Lu Cheng,Yanqiu Wang,Zhuobin Xu,Huihui Wang,Lizeng Gao
Graphical Cleaning wounds : A bimetallic CuCo 2 S 4 nanozyme exhibits enhanced peroxidase-like activity at neutral pH, and can convert H 2 O 2 into highly toxic hydroxyl radicals for killing bacteria. The CuCo 2 S 4 nanozyme effectively facilitates the healing of burn wounds infected with drug-resistant bacteria. Peroxidase-mimicking nanozymes that can generate toxic hydroxyl radicals ( . OH) hold great promise as antibacterial alternatives. However, most of them display optimal performance under strongly acidic conditions (pH 3–4), and are thus not feasible for many medical uses, including burn infections with a wound pH close to neutral. Herein, we report a copper-based nanozyme (CuCo 2 S 4 ) that exhibits intrinsic peroxidase-like activity and can convert H 2 O 2 into . OH at neutral pH. In particular, bimetallic CuCo 2 S 4 nanoparticles (NPs) exhibited enhanced peroxidase-like activity and antibacterial capacity, superior to that of the corresponding monometallic CuS and CoS NPs. The CuCo 2 S 4 nanozymes possessed excellent ability to kill various bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Furthermore, this CuCo 2 S 4 nanozymes could effectively disrupt MRSA biofilms in vitro and accelerate MRSA-infected burn healing in vivo. This work provides a new peroxidase mimic to combat bacteria in neutral pH milieu and this CuCo 2 S 4 nanozyme could be a promising antibacterial agent for the treatment of burn infections.
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