JOURNAL OF PHARMACOLOGICAL SCIENCES
·
2014
·
IF 2.114
Jing Lu,Duanping Sun,Si Gao,Ying Gao,Jiantao Ye,Peiqing Liu
Autophagy is a highly regulated and multi-step biological process that serves to remove damaged cytoplasmic components and organelles. It has been suggested that the activation of autophagy may be a promising therapeutic strategy for cancer treatment by triggering cell death. In this study, we reported that cyclovirobuxine D (CVB-D), an alkaloid component in a traditional Chinese herb, could induce autophagy in the MCF-7 human breast cancer cell line. CVB-D inhibited the viability of MCF-7 cells in a concentration- and time-dependent manner. Activation of autophagy was characterized by transmission electron microscopy, monodansylcadaverine staining, and expression of autophagy marker microtubule-associated protein 1 light chain 3 (LC3). After CVB-D treatment, a clear accumulation of autophagosomes was observed accompanied with elevated LC3 fluorescent puncta. Western blot analysis revealed that CVB-D significantly promoted the conversion from LC3-I to LC3-II and the expression of autophagy-related protein 5 (ATG5), which are both essential for autophagosome formation. On the other hand, CVB-D–induced autophagy and decrease in cell viability could be blocked by 3-methyladenine, a well-established autophagy inhibitor. Moreover, CVB-D attenuated the phosphorylation of Akt and mTOR, two pivotal suppressors in autophagy pathways. These findings shed new light on the pharmacological actions and mechanism of CVB-D and may support the potential utility of autophagy inducers in cancer treatment.
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL NEUROANATOMY
·
2020
·
IF 2.353
Hongqing Liu,Shiqi Li,Cuizhu Yang,Hui Jia,Ziting Gu,Xing Tu,Sumin Tian,Jing Liu,Guoying Li,Yuxin Ma
The senile plaque formed by β-amyloid (Aβ) deposition in the brain is one of the main pathological features of Alzheimer’s disease (AD), and the c-Jun N-terminal kinase (JNK) signaling pathway plays an important role in the pathogenesis of AD. This study aimed to investigate that D-serine may ameliorate motor and cognitive impairment in Aβ injected mice by inhibiting JNK signaling pathway. Firstly, Kunming mice were injected intrahippocampally with Aβ 1-42 to build AD model. The mice were injected intraperitoneally with saline, D-serine, D-amino acid oxidase (DAAO), and Sodium benzoate (BE) for 10 consecutive days, respectively. Subsequently, the motor and cognitive functions of mice were detected by behavioral tests. The silver staining and immunohistochemical methods were used to detect the distributions of Aβ in the hippocampus of mice. 18 F-2-Fluro-D-deoxy-glucose positron emission tomography/computed tomography ( 18 F-FDG PET/CT) scans were performed to detected glucose metabolism of Aβ 1-42 induced lesions. The expressions of relative JNK factors were detected by immunohistochemistry and Western blot methods. These results showed that Aβ severely impaired the motor and memory abilities of mice. The expressions of glial fibrillary acidic protein (GFAP), tumor necrosis factor (TNF-α), N-methyl-D-aspartate receptor 1 (NMDAR1), phospho-JNK (p-JNK), p-c-Jun and activating transcription factor 2 (ATF2) increased significantly. After D-serine treatment, the abilities of movement and memory of mice were improved, and the clearance rate of Aβ was accelerated. The expressions of GFAP, TNF-α, NMDAR1, p-JNK, p-c-Jun and ATF2 decreased significantly. DAAO and BE were administered to further validate these results. Therefore, this study showed that D-serine could alleviate the cognitive impairment of Aβ 1-42 injected mice by inhibiting JNK signaling pathway. These results provide more evidences for the effect of D-serine on AD and relevant mechanism to treat AD.
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DOI
PubMed
高影响因子
CARBON
·
2018
·
IF 7.082
Dongqi Li,Bin Zhang,Chunxuan Zhu,Xiangyu Tian,Zhizheng Zhao,Yu Chen
As the cornerstone of the modern information technology, the development of memory devices with high speed, large capacity, long life time, low power consumption and ease of operation characteristics is beneficial for the fast revolution of the digital world. Using soluble 4-bromobenzene functionalized reduced graphene oxide (RGBr) as two dimensional template, in-situ growing of a novel donor-accept type conjugated polymer “poly[(1,4-diethynyl- benzene)- alt -(4,4′-(quinoxaline-2,3-diyl)bis( N , N -diphenylaniline))](PDQ)” from the RGO surface has been successfully realized via the palladium-catalyzed Sonogashira-Hagihara polymerization. The resultant material called as RGO-PDQ is highly soluble in some common organic solvents . The nonvolatile ternary rewritable memory performance has been observed in an electronic device with the RGO-PDQ film sandwiched between the Al and ITO electrodes. The switching threshold voltages on the ON-1 and ON-2 states are −0.74 and −1.44 V, respectively. The achieved current ratio of OFF: ON-1: ON-2 is about 1: 10: 10 4 . The memory effect of the Al/RGO-PDQ/ITO device on the ON-1state can be assigned to the contribution of the PDQ moieties in the structure of RGO-PDQ. By using conductive atomic force microscopy (C-AFM) technique, the conductive nature of the RGO-PDQ film-based device was in-situ observed.
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DOI
高影响因子
Nano Research
·
2016
·Vol. 9
·期 12
·3683-3694
·
IF 8.893
Deng Jun,Zheng Honghao,Zheng Xiaowen,Yao Mengyun,Li Zheng,Gao Changyou
Chirality is one of the most distinctive biochemical signatures of life, and plays crucial roles in maintaining normal functions of living cells or organisms. Pioneering work from another group has demonstrated the dependency of cell differentiation on the chirality of nano-coated substrates, but the effect of the chiral surface of nanoparticles on stem cell fates has not been investigated. In this study, the influence of molecular chiral poly(acryloyl-L(D)-valine) (L(D)- PAV)-anchored gold nanoparticles (L(D)-PAV-AuNPs) on the differentiation of mesenchymal stem cells (MSCs) was investigated. Though osteogenic differentiation of MSCs was not affected by D-PAV-AuNPs, it was significantly promoted by L-PAV-AuNPs in terms of calcium deposition, alkaline phosphatase (ALP) activity, and expression of collagen type I and osteocalcin (OCN) at both mRNA and protein levels. L-PAV-AuNPs could activate the P38 mitogen-activated protein kinase (MAPK) pathway, and may exert mechanical stress on MSCs because of high amounts of internalization. These results provide new insights on surface chirality at the nanoscale as a direct regulator to guide the differentiation of MSCs, and the use of these nanomaterials for strategic regenerative medicine.
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2022
·
IF 9.965
Xinyi Li,Jingjing Liu,Qinghai Cai,Ziwang Kan,Song Liu,Jingxiang Zhao
Modulating the microenvironment of single-metal active sites holds excellent promises for developing highly efficiently oxygen electrocatalysts. Herein, by combining theoretical predictions and experiments, we reported a general strategy to engineer the electronic properties of iron-nitrogen-carbon (FeN 4 /C) catalysts via the incorporation of the boron (B) atom for achieving improved catalytic activity in oxygen electrocatalysis. Our theoretical results revealed that B modulation effectively tunes the d-band center of the iron (Fe) active site to optimize its adsorption strength with oxygenated species, greatly enhancing oxygen reduction reaction (ORR) and oxygen evolution reactions (OER) activity. Our experimental measurements then confirmed the above theoretical predictions: the as-synthesized B-doped FeN 4 /C (Fe-N 4 -B) material can perform as an eligible bifunctional catalyst for ORR and OER in alkaline media, and its catalytic activity even outperforms the commercial noble metal benchmarks. The present findings provide a promising strategy to further design the advanced catalysts for a wide range of electrochemical applications.
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Xin Tao,Jingyu Ren,Danjun Wang,Hua Huang,Yani Li,Di Guo,Baoqin Shan,Yu Liu,Jian Wang,Yanzhong Zhen,Zhirui Niu
The low activity of photocatalysts is mainly due to inefficient charge separation and low production of the active species. To overcome this challenge, we constructed an S-scheme CdIn 2 S 4 /MnZnFe 2 O 4 (CIS/MZFO) heterostructure, where the strong interface of the heterostructure leads to an upshift of the d-band center. CIS/MZFO shows higher charge transfer efficiency and oxygen adsorption/activation performance compared to CIS and MZFO separately, thereby increasing the production of ·O 2 − and h + active species. As a result, it demonstrats efficient photocatalytic degradation of tetracycline hydrochloride. The optimized CIS/MZFO heterostructure also shows good tolerance to various factors, such as inorganic ions, organic acids, pH and real water environments. The heterostructure also exhibits excellent magnetic recyclability and stability. Additionally, we propose a photocatalytic degradation pathway for tetracycline hydrochloride and evaluate the ecological toxicity that may arise from intermediates. This study provides an effective approach for the design of advanced photocatalysts.
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Zhixiang Cai,Hongbin Zhang
The chain conformational change in curdlan during carboxymethylation was investigated using nuclear magnetic resonance (NMR), circular dichroism (CD) spectroscopy, and atomic force microscopy (AFM). The distributions of carboxymethyl substituents within anhydroglucose unit (AGU) of CMCD were found to follow the order of OH (6) > OH (4) > OH (2) for CMCD with a low DS and OH (6) > OH (2) > OH (4) for CMCD with relatively high DS. The increased carboxymethylation level induced the chain conformation transition of curdlan from triple helix to random coil in water. The DS of 0.25 was the critical value of chain conformation transition, below which CMCD chains were triple helices. For DS larger than 0.25, CMCD existed in the state of random coils. The intermolecular hydrogen bonding between C2 hydroxyls in AGU sustained the triple helical conformation and stiffness of the polymer chain, which weakened with the increase in DS.
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DOI
PubMed
高影响因子
ENERGY & FUELS
·
2020
·
IF 3.421
Chunmei Deng,Mingyuan Zhu
In this research, we have prepared a new type of multistage aperture nitrogen-doped carbon materials [CN-x, (x = 600, 700, 800, 900, 1000)] by one-step carbonization at different calcination temperatures. The structure of the CN-x is characterized by X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller, Scanning electron microscopy, and Raman spectroscopy. The effects of pore structure, nitrogen morphology, and defects on the adsorption properties are analyzed. We have also studied the desulfurization activity of CN-x for dibenzothiophene (DBT). Experimental data show that CN-900 has very good adsorption desulfurization activity, exhibiting a theoretical Langmuir adsorption capacity of up to 37.28 mg/g. When the DBT concentration is 100 ppm, CN-900 reaches a DBT removal rate of over 99%. The effects of calcination temperature and nitrogen content on the adsorption properties of the CN-x are examined. The experimental results show that the nitrogen content is positively correlated with the adsorption performance. The cheap and readily available d-(+)-glucosamine hydrochloride is used as the precursor of the CN-x, and the adsorbent prepared by a simple one-step carbonization method has good application prospects in the desulfurization process.
研究领域:
材料科学
·
环境工程
·
化学工程
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DOI
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2015
·
IF 2.871
Xia Yi,Peng Zhang,Jiaoe Sun,Yi Tu,Qiuqiang Gao,Jian Zhang,Jie Bao
Pediococcus acidilactici TY112 producing l -lactic acid and P. acidilactici ZP26 producing d -lactic acid, were engineered from the wild-type P. acidilactici DQ2 by ldhD or ldh gene disruption , and the robustness of the wild-type strain to the inhibitors derived from lignocellulose pretreatment was maintained well. In simultaneous saccharification and fermentation (SSF), 77.66 g L −1 of l -lactic acid and 76.76 g L −1 of d -lactic acid were obtained at 25% (w/w) solids content of dry dilute acid pretreated and biodetoxified corn stover feedstock. l - and d -Lactic acid yield and productivity were highly dependent on the inhibitor removal extent due to the significant down-regulation on the expressions of ldh and ldhD encoding lactate dehydrogenase by inhibitor, especially syringaldehyde and vanillin at the low concentrations. This study provided a prototype of industrial process for high titer l - and d -lactic acid production from lignocellulose feedstock.
研究领域:
微生物工程
·
生物化学工程
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DOI
高影响因子
FUEL
·
2024
·
IF 6.7
Fengrui Li,Xiao Dai,Linfeng Zhang,Huadong Wu,Jianding Li,Jia Guo,Qun Yi
Conjugated microporous polymers (CMPs) with a donor–acceptor (D-A) molecular structure usually express the properties of well light-induced electron/hole separation efficacy and high photocatalytic activity. The triphenylamine (TPA) with a special three-dimensional propeller structure was chose as the electron donor, and the dibenzothiophene sulfone (BTDO) groups as the chain scaffold and electron acceptor to construct and prepare a unique “macrocyclic structure” D-A type CMPs photocatalyst (TPA-BTDO) for photocatalytic hydrogen production (PHP). In order to further improve the conjugation degree of the CMPs and enhance the electron transfer capacity and light absorption, the thiophene units was introduced as “π-bridge” to form a macrocyclic D-π-A type CMPs photocatalyst (TPA-T-BTDO). The results show that the addition of thiophene greatly improves the coplanarity between the donor–acceptor units, and the prepared TPA-T-BTDO-1 exhibited an amazing PHP efficiency of 65.41 mmol h −1 g −1 in the visible light region. More importantly, the PHP efficiency also reaches up to 39.63 mmol h −1 g −1 under the natural light conditions. Both the characterizations and DFT simulation results prove that the twisted structure of the TPA donor decreases the dihedral angle between the benzene rings of BTDO and TPA, which improves the coplanarity of polymer skeleton and then prevents the charges transfer along the polymer chain for recombination. This study highlights the significant influence of the geometry of the electron donor on the photocatalytic activity, as well as the strategy of the structure adjusting by adding suitable bridges between the donor–acceptor units.
研究领域:
材料科学
·
光催化
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DOI
高影响因子
POLYMER SCIENCE SERIES A
·
2021
·Vol. 63
·期 3
·267-274
·
IF 1.206
Jun Shao,Tang Juan,Pu Shouzhi,Hou Haoqing
Abstract Blending of poly( L -lactic acid)/poly( D -lactic acid) (PLLA/PDLA) leads to the formation of poly(lactide) stereocomplexation (PLA SC), which exhibits higher melting temperature and faster crystallization rate. The crystallization behavior of PLA homochiral crystallization (HC) under the existence of PLA SC was also widely investigated. During the variable processing, the SC with different morphologies would produce, and these SC could exert different influences on the subsequent formation of HC. However, the effects of SC developed in various conditions on the formation of HC are not compared yet and are also not clear until now, and which is important for the application of PLLA/PDLA blends. In this paper, the PDLAs with different molecular weights were blended with PLLA at a weight ratio of PLLA/PDLA 90/10, and the crystallization behaviors of PLLA HC from different conditions were investigated by differential scanning calorimetry (DSC). Results indicated that, incorporating a small amount of PDLA accelerated the formation of PLLA HC significantly regardless of the SC developed from the solution evaporation or the melt. The higher crystallization rate of HC was received in the PLLA/PDLA specimens with lower molecular weight of PDLA. For the specimens crystallized from the partially melting (most of SC developed during solution evaporation), the crystallization rate was higher than that corresponding specimens crystallized from the completely melting state (the SC were produced from the melt). This discrepancy would be due to variable SC morphologies produced during diverse processing.
研究领域:
高分子材料学
·
材料科学
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DOI
高影响因子
CRYSTENGCOMM
·
2021
·Vol. 23
·期 22
·3989-3994
·
IF 3.545
Anqi Li,Xiaofan Ma,Jie Ma,Qingyi Yuan,Jun Zhang,Xiaopeng Xuan
Herein we reported a simple and economical method for fabricating a portable separation device based on exfoliated 2D-metal–organic framework (2D-MOF) nanosheets. This device is composed of a layered Cu-MOF as a filter and a syringe filter as the holder. Benefiting from the structural characteristic of the 2D-MOF nanosheets, the separation experiment reveals that this strategy can exhibit high efficiency and selectivity for dyes with different sizes. This device can be used disposably for a special purpose due to the low cost, simple manufacturing process in an emergency.
研究领域:
材料科学
·
环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）