BIOORGANIC CHEMISTRY
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2026
·
IF 5.1
Gaiqian Song,Ayixia Abudula,Moyisa Simayi,Hongning Liu,Hao Lin
Taxol remains a cornerstone therapy for non-small cell lung cancer, however, the development of acquired resistance continues to pose a significant clinical hurdle. Glutathione S-transferase P1 (GSTP1) and P-glycoprotein (P-gp) have been identified as key mediators of this multi-drug resistance phenotype. Consequently, the dual-target inhibition of these proteins represents a promising therapeutic strategy to circumvent chemoresistance. Here, pentagalloylglucose has been identified as a potential dual GSTP1/P-gp inhibitor through virtual screening. Molecular dynamics simulations validated the stable binding conformations of pentagalloylglucose with both target proteins. Furthermore, microscale thermophoresis and cellular thermal shift assays confirmed the direct binding interaction between pentagalloylglucose and GSTP1, while enzymatic assays demonstrated its potent inhibitory efficacy against the enzyme. Notably, treatment with pentagalloylglucose significantly enhanced the intracellular accumulation of Rhodamine 123, thereby indicating effective inhibition of P-gp mediated efflux. This effect culminated in a marked restoration of taxol sensitivity in A549/Taxol resistant cells. Additionally, pentagalloylglucose treatment inhibited cell metastasis in a concentration-dependent manner, accompanied by the down-regulation of epithelial-mesenchymal transition-related genes. Transcriptomic analysis further revealed that pentagalloylglucose modulates multiple signaling pathways implicated in cell proliferation and metastasis. Collectively, these findings establish pentagalloylglucose as a promising dual-target inhibitor for overcoming taxol resistance. The strategy of simultaneously targeting GSTP1 and P-gp offers a viable approach for the treatment of drug-resistant lung adenocarcinoma, providing a robust scientific foundation for future drug development and structural optimization.
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HYDROMETALLURGY
·
2026
·
IF 6.3
Gengjin Zhang,Yanfang Huang,Longfei Jia,Tianze Chen,Yizhuang Wang,Shengpeng Su,Wenjuan Wang,Hu Sun,Guihong Han
Novel peroxo-complexation strategy was used to purify Nb-rich solution. • NbOF 5 2− transformation to NbO 2 F 5 2− greatly lowers its binding energy with MIBK. • Selectivity coefficient for Sb over Nb was enlarged by over 2300 times. • Nb 2 O 5 product met the high-purity specifications regarding Ta and Sb impurities. The efficient separation of Ta and Sb from Nb in hydrometallurgical processes remains a significant challenge due to their similar chemical behaviors. In this study, a novel peroxo-complexation strategy was introduced to enhance the selectivity of methyl isobutyl ketone (MIBK) extraction in an HF-H 2 SO 4 system. With the addition of H 2 O 2 , the extraction efficiencies of Ta and Sb reached 99.6% and 99.7%, respectively, while the co-extraction of Nb was only 2.32%. The selectivity coefficients of Sb/Nb and Ta/Nb significantly increased from 0.63 and 34.7 to 1461 and 6501, respectively. Stepwise stripping using H 2 SO 4 and pure water effectively recovered Nb and Ta/Sb from the loaded organic phase. Raman spectroscopy and quantum chemical calculations elucidated the mechanism of the enhanced separation selectivity. The peroxo-ligand selectively coordinates with Nb to transform it from NbOF 5 2− to NbO 2 F 5 2− , thereby weakening the negative surface potential of the fluorine atoms bonded to Nb. The binding energy between Nb ions and MIBK decreases from −172 kJ mol −1 to −104 kJ mol −1 , and thus the disparity in the affinity of Nb ions and Ta/Sb ions for MIBK is magnified. Overall, this peroxo-coordination approach provides an efficient and simple pathway for the effective purification of Nb solutions. Download: Download high-res image (176KB) Download: Download full-size image
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High Voltage
·
2026
·
IF 5.7
Xi Zhu,Liyan Wang,Xiuhan Guan,Tianhe Lu,Zhangliang Shen,Jiarui Bao,Hongtu Cheng,Zhi Fang
Polymer insulation exposed to natural environment inevitably face challenges from material ageing and surface insulating degradation. Surface treatment is an effective method to improve material performance, but traditional processing methods such as room temperature vulcanised silicone rubber (RTV) coating, are subjected to coating degradation after long time operation. Herein, we fabricated a stable cross-linked Si-containing (–SiO 4 ) film on polymethyl methacrylate (PMMA) insulation via eco-friendly atmospheric pressure plasma techniques, and verified its thermal stability performance. By addition of hexamethyldisiloxane (HMDSO) and fluorescent agent in plasma, fragmenting–crosslinking of HMDSO as well as surface functionalising by plasma-enabled reactions lead to the fabrication of fluorescent Si-containing film. Accelerating ageing test demonstrates that the plasma-modified PMMA remains steady hydrophobic performance after ageing. Although the surface insulating performance decreases with the ageing, the aged material still remains the higher flashover voltage compared with the original. It reveals that the Si-containing film could convert thermal oxygen reactions into itself crosslinking process with the shift of –SiO 2 to –SiO 4 groups, which eventually leads to the variation of material surface performance. Overall, the plasma-assisted Si-containing film for surface modification has satisfactory thermal stability, which offers a new avenue for insulation enhancement and service life prolonging.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 32
·25542-25553
·
IF 6.7
Bi Yongzhao,Pei Lizhen,Sun Xingming,Luo Jin,Liu Xialei,Huang Yan,Sun Baoguo,Liang Li,Zhang Yuyu
Bitter peptides (BPs) have a wide
range of applications in the
food and pharmaceutical industries. This study identified four food-derived
BPs (TPLVDR, VLY, LEEL, and FYL) as novel agonists of the human bitter
taste receptor hTAS2R4, using a rapid cell-based screening approach.
Activation of hTAS2R4 by these peptides inhibited downstream cAMP
signaling, with effective concentration values of 191.99, 222.96,
297.17, and 63.96 μM, respectively. Molecular docking and sequence
alignment across 25 bitter taste receptors highlighted eight potential
binding sites. Subsequent site-directed mutagenesis validated F62 2.57 , F69 2.64 , V85 3.29 , S184 5.461 , Y250 6.56 , and L266 7.38 as key binding sites
for hTAS2R4 activation by BPs. These findings provided crucial evidence
for the molecular mechanism of peptide recognition and signal transduction
by hTAS2R4 activation.
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高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2026
·
IF 6
Wanmei Zhou,Zixin Wang,Chengdong Nie,Haiting Tian,Xiaoyi Duan,Lina Fu,Quan Zhou,Litao Wang,Yujie Fu
Triple-negative breast cancer (TNBC) lacks effective tumor-selective strategies, particularly limiting ferroptosis-based therapies that depend on oxidative stress. Here, we develop a structurally reinforced ternary redox-cycling nanoreactor that integrates Zr 4 ⁺-stabilized zeolitic imidazolate framework-8 (ZIF-8) with redox-active Cu/Mn centers and ultrasmall Au nanodots, while co-loading paclitaxel (PTX) for acidic tumor microenvironment-responsive release. The Cu-Mn-Au interfaces enable rapid electron shuttling, sustaining multivalent metal cycling, accelerating Fenton-like reactive oxygen species (ROS) generation, depleting GSH, and promoting lipid peroxidation. Density functional theory (DFT) calculations further support its self-perpetuating redox mechanism. Dual surface modification with 4 T1 cell membranes and folic acid confers homotypic and receptor-mediated targeting. Mechanistic studies reveal coordinated GPX4/SLC7A11 suppression, PTX-enhanced cell-cycle arrest, and NCOA4/HO-1-mediated ferritinophagy, collectively amplifying ferroptosis. Unlike conventional metal–organic frameworks (MOFs), this Zr-reinforced trimetallic system maintains continuous redox cycling under reductive conditions. Overall, the nanoreactor achieves sustained ROS amplification, iron-homeostasis remodeling, and enhanced tumor-selective ferroptosis, offering a promising therapeutic strategy for TNBC. Download: Download high-res image (197KB) Download: Download full-size image
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Pharmaceuticals
·
2026
·Vol. 19
·期 8
·1261
·
IF 5.7
Yongxin Sun,Hao Tan,Jingyu Zhang,Zengyu Zhang,Shuang Huai,Manli Wang
Background:Hyperuricemia is a well-established risk factor for both chronic kidney disease and gout. Currently available urate-lowering drugs, however, are frequently compromised by hepatorenal toxicity and gastrointestinal adverse effects. Cinnamaldehyde, the principal bioactive constituent of the traditional Chinese medicine cinnamon, has documented renoprotective properties, yet its integrated effects on hyperuricemia-associated renal injury remain incompletely defined.Objectives:This study investigated the therapeutic effects of cinnamaldehyde on hyperuricemia and explored the molecular mechanisms involved.Materials and Methods:An in vivo hyperuricemia model was established in KM mice by co-administration of potassium oxonate and hypoxanthine for 14 consecutive days, and an in vitro injury model was generated by exposing HK-2 human renal tubular epithelial cells to uric acid. For the in vivo study, mice were divided into five groups (control, model, febuxostat, low-dose CA, and high-dose CA;n= 9 per group) and were treated by oral gavage. Serum biochemical indices, renal and intestinal histopathology, inflammatory cytokines, renal urate transporter proteins (OAT1, OCT2, ABCG2, and SLC2A9), and components related to AIF1 and CMPK2/NLRP3 inflammasome signaling (ASC, caspase-1, IL-18, and IL-1β) were examined. Western blotting, qPCR, immunofluorescence, apoptosis analysis, mitochondrial membrane potential assessment, transmission electron microscopy, siRNA-mediated knockdown, and 16S rRNA gene sequencing were used to characterize the relevant mechanisms.Results:CA reduced serum uric acid (p≤ 0.001 vs. model), creatinine (p≤ 0.01 vs. model), and blood urea nitrogen (p≤ 0.01 vs. model), alleviated renal and intestinal histopathological injury, and decreased circulating and renal inflammatory cytokines. Cinnamaldehyde increased ABCG2, OAT1, and OCT2 protein expression and reduced SLC2A9 expression (allp≤ 0.05 vs. model). In vivo and in vitro, cinnamaldehyde suppressed AIF1- and CMPK2/NLRP3-related inflammatory proteins, reduced uric acid-induced apoptosis, and preserved mitochondrial membrane potential and ultrastructure. Fecal 16S rRNA sequencing suggested changes in selected microbial taxa, although alpha-diversity and BrayCurtis-based PERMANOVA/ANOSIM analyses did not demonstrate significant global community separation.Conclusions:These findings suggest that CA alleviates hyperuricemia-associated renal injury by regulating renal urate transporters, attenuating AIF1- and CMPK2/NLRP3-related inflammatory signaling, accompanied by compositional shifts in selected gut microbial taxa that warrant further mechanistic investigation. The study provides experimental support for further evaluation of cinnamaldehyde as a multi-target candidate for hyperuricemia-related renal injury.
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2026
·
IF 19.9
Xinming Wang,Weichang Xie,Zichen Wang,Haonan Zhang,Jia Li,Xingjian Gao,Dingjie Zhou,Huaiyu Jiang,Shixing Huang,Qiang Zhao,Xiaofeng Ye
Repairing anisotropic tissues such as the myocardium requires implanted cells to organize into highly ordered structures. However, achieving controlled cell alignment remains a major challenge for current cell delivery patches. Here, we develop a magnetically aligned, fiber-reinforced extracellular matrix (ECM) patch that directs anisotropic cell organization and supports stem cell implantation. Poly-lactic acid fibers containing Fe 3 O 4 are fabricated via electrospinning and controlled hydrolysis, and are aligned uniaxially within ECM hydrogels using an external magnetic field prior to gelation. The resulting ECM–fiber patches exhibit enhanced mechanical strength and reliably guide the alignment of cardiomyocytes, fibroblasts, and endothelial cells. A beating patch is also created by seeding human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs). In a nude mouse myocardial infarction (MI) model, implantation of iPSC-CMs@ECM–fiber patches preserves cardiac function, reduces apoptosis, limits fibrosis, and enhances vascularization. Implanted iPSC-CMs demonstrate high viability and low immunogenicity. Molecular profiling reveals transient modulation of early inflammatory responses post-MI, probably mediated through IL-17, IL-3, and IL-1β pathways. Overall, the ECM–fiber patch provides a versatile platform for delivering highly organized cells for in vivo tissue repair.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Xiaoyu Cao,Sisheng Li,Qianqian Xu,Zilun Lei,Bo Jiao,David Julian McClements,Hai Yan
pH-responsive hydrogel microcapsules were fabricated from pea protein isolate (PPI) and low-methoxyl pectin (LMP) for the targeted intestinal delivery of Lacticaseibacillus paracasei YWC-01, a probiotic capable of biodegrading uric acid precursors in vitro . Microgels with interpenetrating networks were fabricated via emulsion-templating: probiotics, PPI, LMP, CaCl₂, and glucono-δ-lactone were emulsified in oil and crosslinked by Ca 2+ . Optimized formulation (5% w / v PPI) achieved 94.80% encapsulation efficiency. Microgel size (43–63 μm) was controlled by adjusting Span 80 surfactant concentration. FTIR confirmed probiotic encapsulation via noncovalent interactions, while TEM revealed bacterial clustering within hydrogel voids. In vitro digestion demonstrated exceptional protection: encapsulated probiotics maintained >9 log 10 CFU/g viability (<0.4 log reduction), versus 3.2 log reduction in free cells. Metabolomics indicated encapsulation preserved bioactivity and significantly upregulated functional metabolites potentially enhancing gut microbiota modulation and anti-inflammatory effects. This work establishes an effective oral delivery platform that significantly enhances probiotic gastrointestinal survival and enables targeted release.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2026
·Vol. 91
·期 2
·e70897
·
IF 4.1
Hongyu Zhang,Da Wang,Xiangheng Cao,Baiyu Zheng,Ruiyang Qiu,Wenhui Xu,Koukou Duan,Shuping Wei,Mengge Zhao,Shichao Han
With the rapid expansion of the probiotics industry, the management of spent culture media has emerged as a pressing concern. However, it also represents a potential resource for functional food development. In this study, we investigated and screened anti-hyperuricemia (HUA) metabolites of Lactobacillus paracasei (LP) from spent media (META). Administration of META significantly reduced serum uric acid (UA) (SUA) levels ( p < 0.05), improved kidney function, and alleviated inflammatory infiltration. Furthermore, META modulated UA metabolism by regulating key enzymes (xanthine oxidase [XOD] and adenosine deaminase [ADA]) and urate transporters (organic anion transporter 3 [OAT3], glucose transporter 9 [GLUT9], and urate transporter 1 [URAT1]), while mitigating HUA-induced inflammation through the Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) pathway. To identify bioactive compounds, untargeted metabolomics based on UHPLC-QTOF-MS/MS detected 361 upregulated metabolites. Network pharmacology and molecular docking (MD) analyses further highlighted potential candidates, including 2-isopropylmaleate and ricinoleic acid methyl ester. Collectively, these findings suggest that probiotic culture media waste may serve as a promising natural source for the development of anti-HUA functional foods. Further studies are warranted to assess the bioavailability and efficacy of these metabolites both in vitro and in vivo.
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Horticulturae
·
2026
·Vol. 12
·期 1
·106
·
IF 3
Yunxuan Liu,Xinjia Wang,Rong Lian,Yan Zhao,Yurong Zhou,Yuan Yu,Wenqin She,Zhixiong Guo,Heli Pan,Tengfei Pan
The granulation of pomelo (Citrus maxima) juice sacs severely compromises fruit quality and is closely associated with lignin accumulation, a process catalyzed by peroxidases (PODs). Analysis of ‘Sanhong’ pomelo juice sacs collected 175–215 days after flowering revealed that bound peroxidase (BPOD) activity paralleled changes in lignin content, suggesting a potential role for BPOD in lignin biosynthesis. A total of 71CmPODgenes were identified in the pomelo genome through integrated HMMER and BLAST analyses. Among them,CmPOD52was selected for functional characterization based on its alkaline peroxidase properties, absence of a CE domain, predicted extracellular localization, and gradually increasing expression pattern revealed by RT-qPCR. Its transient overexpression in ‘Sanhong’ pomelo juice sacs for 36 h increased BPOD activity 2.06-fold (p< 0.01) compared to the empty vector control, indicating thatCmPOD52may be a BPOD gene. The recombinant CmPOD52 protein was expressed in a prokaryotic system, purified, and used in enzymatic assays with sinapyl alcohol as the substrate. The recombinant CmPOD52 protein, assayed at 272 nm with controls (substrate-only blank and heat-inactivated protein), showed an activity of 13.67 ± 0.9 U. The experimental group showed new products, identified by mass spectrometry as sinapyl alcohol dimers, thus suggesting that the recombinant protein catalyzes the dehydrogenation and polymerization of sinapyl alcohol monomers. This study identifiedCmPOD52, a gene potentially involved in lignin polymerization in pomelo juice sacs, offering a key candidate for further in vivo validation.
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高影响因子
Food & Function
·
2026
·
IF 6.3
Hongyu Zhang,Chaoran Li,Tianjiao Li,Fuyu Li,Xiangheng Cao,Yang Yang,Da Wang,Mingyou Yuan,Qiuyi Li,Xuebo Liu,Mengge Zhao
Hyperuricemia (HUA) is a worldwide metabolic disorder. We developed a functional fermented beverage by fermenting Aronia melanocarpa (AR) with the urate-lowering probiotic Lactobacillus paracasei N1115 (LP). Firstly, untargeted metabolomics revealed that LP balanced its aroma and imparted a lactic flavor to AR and increased the concentrations of certain compounds with anti-HUA properties (e.g., caffeic acid and shikimic acid). In HUA mice, AR, fermented AR (FAR) and sterilized FAR (FARS) reduced serum uric acid, improved renal function, and suppressed inflammation via the TLR4/MyD88/NF-κB pathway. They also down-regulated the levels of XOD, ADA and URAT1. The fermented beverage showed superior efficacy, enhancing XOD inhibition by about 30% and additionally inhibiting GLUT9. FAR and FARS increased fecal butyrate, likely by enriching Faecalibaculum and Anaerostipes. Because the anti-HUA patterns of FAR and FARS were similar, and no colonization of Lactobacillus was observed, the postbiotics may serve as the primary factors contributing to the enhanced anti-HUA activity of the fermented beverage.
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Journal of Water Process Engineering
·
2026
·
IF 6.6
Xiang Luo,Hongwei Chen,Hongjian Shen,Ling Zhang,Jinbo Chen,Shuai Liu,Bo Gao,Honglei Zhang,Yawei Gu
Zero-valent iron (ZVI) technology is regarded as one of the most efficient approaches for remediating groundwater contaminated with chlorinated hydrocarbons (CHCs). Despite its highly reactive nature, the corrosion of ZVI in aqueous solution readily results in surface passivation, which diminishes its reactivity. ZVI doped with heteroatoms such as carbon (C), nitrogen (N), and sulfur (S) has displayed excellent performance in trichloroethylene (TCE) degradation. Nevertheless, the effects of these heteroatoms on ZVI materials, along with their various degradation mechanisms, remains ambiguous. In this study, C-mZVI bm , N-mZVI bm , and S-mZVI bm were prepared, respectively, via a mechanochemical method and utilized for TCE degradation. The dechlorination reaction facilitated by each material was thoroughly investigated both theoretically and experimentally, focusing on TCE adsorption affinity, the dechlorination process, hydrogen evolution reaction (HER), electron efficiency, and lifetime. Among the three materials, S-mZVI bm exhibited 100% TCE removal in less than 24 h., as well as improved electron efficiency and lifetime, primarily attributed to its high TCE adsorption energy and the suppression of HER. These integrated theoretical and experimental results indicate that S-mZVI bm nanoparticles are a promising and innovative material for in situ groundwater TCE remediation.
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危险品化学品经营许可证（带存储）