CURRENT ORGANIC SYNTHESIS
·
2024
·Vol. 21
·期 1
·47-60
·
IF 1.7
Zhang Cuizhi,Fan Yanchao,Wang Sijia,Liu Shaojie,Chu Xiaomeng,Tang Erjun
Introduction: L-proline is an efficient chiral small-molecule organocatalyst for the direct asymmetric aldol reaction between unmodified acetone and a variety of aldehydes. Methods: However, it is difficult to separate from the reaction medium for reuse. In this work, polyacrylic
acid (PAA) supported The acylation reaction between L-hydroxyproline and PAA prepared lproline (P(AA-co-PA)) catalysts with various catalyst loadings. Fourier characterized them transforms infrared spectroscopy, nuclear magnetic resonance spectrum, gel permeation chromatography and thermogravimetry
analysis. Results: These macromolecular catalysts were used to catalyze acetone and benzaldehydes' direct asymmetric aldol reaction. The influence of the catalyst structure on the catalytic performance was studied, and the reaction conditions were optimized. Conclusion: The results
showed that P(AA-co-PA) with 50 mol% catalyst loading had excellent catalytic performance, much higher than that of L-proline and L-hydroxyproline. Its recovery was achieved by simple filtration. After being reused 7 times, its catalytic performance was still higher than that of L-proline.
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ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Hongjie Zong,Bo Wang,Guifei Li,Shifeng Yan,Kunxi Zhang,Yufeng Shou,Jingbo Yin
Currently, biodegradable hydrogels are one of the most promising materials in tissue engineering. From the perspective of clinical needs, hydrogels with high strength and minimally invasive implantation are preferred for the reconstruction of load-bearing tissues. In this work, a biodegradable, high-strength, and injectable hydrogel was developed by one-step photo-cross-linking of two biomacromolecules, polyethylene glycol acrylated poly(l-glutamic acid) (PLGA-APEG) and methacrylated gellan gum (GG-MA). The hydrogels, named as PLGA/GG hydrogels, exhibited high mechanical properties. The compression stress of the hydrogels was 0.53 MPa, and the fracture energy was 7.7 ± 0.2 kJ m–2. Meanwhile, the storage modulus could reach 44.0 ± 0.6 kPa. The hydrogel precursor solution loaded with adipose-derived stem cells (ASCs) was subcutaneously injected into C57BL/6 mice and then cross-linked via in situ transdermal irradiation, which demonstrated the excellent injectability and subcutaneous gelatinization of PLGA/GG hydrogels as cell carriers. Furthermore, three-dimensional encapsulation of ASCs in hydrogels after 7, 14, and 21 days showed outstanding cytocompatibility, and the viability of ASCs was up to 84.0 ± 1.7%. The PLGA/GG hydrogels exhibited ideal behaviors of degradation, with 60 ± 5% of hydrogels degraded in phosphate buffered solution (PBS) after 11 weeks. H&E staining demonstrated that the hydrogels degraded gradually after 6 weeks and supported tissue invasion without inflammatory reactions, which indicated the laudable biodegradability of hydrogels. Hence, the biodegradable and high-strength hydrogels with well-performed injectability and biocompatibility possessed high potential applications in tissue engineering, especially in load-bearing tissue regeneration.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 18
·7981-7997
·
IF 3.553
Zhang Xiaoyong,Liu Huihui,Yue Lipei,Bai Yongping,He Jinmei
When it comes to bonding in an aqueous environment, intertidal marine organisms such as mussels, sandcastle worms, barnacles and polychaetes are providing inspiration for the assembly of synthetic molecules into the polymeric adhesives. Although intense demands in various life or industrial applications and many mussel-inspired systems have been shown to develop coatings and hydrogels, exploring the mimetic mussel adhesive system through the control of composition and polymer molecular weight is demonstrated less often. In this report, we showed a simple approach to synthesize mussel-inspired adhesive, P( N -butyl acrylate- co - l -Dopa-methacrylate anhydride), which possesses characteristics of simple synthesis, easy-using, no extra-crosslinker need, and excellent adhesion behaviour on diverse substrates. Adhesion behaviour was examined using low- and high-surface-energy substrates (e.g. PTFE and steel, respectively). Furthermore, the fabricated adhesives exhibited excellent underwater adhesive behaviour for steel in various aqueous solutions, including ion ((IO 4 ) − and Mn 3+ ), acid and alkali solutions (pH = 4, 6.7 and 8, respectively). The outstanding fabricated adhesives take advantage of the catechol functionality and wettability of the resulting polymer. These insights should help us to design next-generation underwater adhesive systems as well as open up a number of future research designs for mussel-inspired adhesives with performance not accessed previously.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuai Li,Jianqin Zhang,Hao Wang,Xingyun Zhao,Lin Yang,Lin Chai,Yangyang Liu,Zhangwu Zhao,Bernard Moussian,Abeer El Wakil,Kun Yan Zhu,Zhanghua Zeng,Jianzhen Zhang
RNA interference (RNAi)-based biopesticides, recognized by Science as one of the top ten scientific breakthroughs of 2024, are poised to drive the third revolution in pesticide development. However, the practical applications of RNAi face critical challenges, particularly in overcoming delivery barriers, such as double-stranded RNA (dsRNA) degradation and poor cellular uptake. In this study, we developed a biologically derived melanin nanoparticles (MNP) functionalized with poly- l -lysine (PLL) to deliver dsRNA in Locusta migratoria , a major agricultural pest. The functionalized melanin nanoparticles (MNP-PLL) self-assemble with dsRNA to form MNP-PLL/dsRNA through electrostatic interactions. We evaluated their stability under different environmental conditions, such as in midgut fluid, under various temperature and UV irradiation, demonstrating significantly enhanced resistance to degradation. Bioassays showed substantial improvements in RNAi efficiency and insect mortality. Using fluorescent labeling and pharmacological inhibitors, we further investigated the cellular uptake mechanisms. In contrast to naked dsRNA, chitosan/dsRNA, carbon quantum dots/dsRNA and Spc/dsRNA, MNP-PLL/dsRNA activated three distinct endocytic pathways: clathrin-mediated endocytosis, caveolin-mediated endocytosis, and macropinocytosis, facilitating efficient cellular internalization. Acute toxicity assays confirmed excellent biocompatibility and safety of MNP-PLL to the Western honey bee and zebrafish. This study represents the first application of PLL-functionalized melanin as an efficient nanocarrier to generate MNP-PLL/dsRNA for agricultural pest control, highlighting their strong potential as a next-generation dsRNA delivery platform for sustainable pest management.
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ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Feixuan Li,Yu Xiong,Dongping Zhan,Xinxin Xiao,Shizhen Wang
This study presents a hybrid system combining MIP-202 (Zr⁴⁺-Asp-coordinated framework) immobilized L-phenylalanine dehydrogenase with polymerized methylene blue (PMB) based electrodes. MIP-202 acted as a biocompatible framework that preserved enzymatic activity. PMB modulated the oxidation of the enzyme cofactor NADH into NAD + via proton-coupled electron transfer (PCET), reducing overpotentials. Such a biohybrid, operated at a relatively low potential of +0.15 V vs. SCE, enables proof-of-concept electrochemical biosensing of L-phenylalanine (L-Phe) and bioelectrosynthesis of phenylpyruvate (PPA). Both applications are of great significance, as L-Phe is an important biomarker for the diagnostics of phenylketonuria, while PPA is a common α-keto acid. It demonstrated a limit of detection (LOD) of 17.03 μM for L-phe and a ca. two-fold increase in PPA production compared to the enzyme-only system without cofactor regeneration.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lei Nie,Xiaoyue Ding,Yingsong Zhao,Peng Ding,Meili Zhao,Yuanna Sun,Yanfang Sun,Guohua Jiang
Multifunctional hydrogel dressings, with their robust antibacterial and anti-inflammatory properties, represent a great potential in biomaterials for wound care. Injectable and self-healing hydrogels that mimic the natural extracellular matrix of tissues offer a promising strategy for effective wound repair. This study designs a hydrogel based on the formation of a dynamic cross-linking network within the hydrogel matrix through the Schiff base reaction between the synthesized polymers L-hydroxyproline-conjugated chitosan (LHCS) and oxidized pullulan (OPL). The fabricated LP hydrogels preserve the porous interconnected microstructure, excellent self-healing capacity, and injectability, suitable swelling capability, robust mechanical strength, and outstanding tissue adhesion. Moreover, curcumin was encapsulated in Polyether F127 (Pluronic F127) micelles to obtain curcumin loaded PF127 (Cur-PF127) micelles and then incorporated into LP hydrogel to fabricate the composite hydrogels. The composite hydrogels exhibit effective antibacterial properties and antioxidant abilities, admired hemostatic effect and hemocompatibility, excellent cytocompatibility and cell pro-migration ability. In addition, in vivo evaluation confirmed that curcumin loaded hydrogels could facilitate the regeneration of epithelial and hair follicles, increase the collagen production, alleviate the inflammatory response via the downregulation of the tumor necrosis factor-α (TNF-α), and reduce the fibrosis, thus promote the wound repair process. This work offers a promising facile approach for the development of the composite hydrogel as wound dressing in wound healing applications.
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PLoS One
·
2025
·Vol. 20
·期 5
·e0322835
·
IF 2.6
Fujie Xu,Xiaohang Shan,Jialin Li,Jing Li,Jiqiao Yuan,Daozeng Zou,Manyuan Wang
Artemisinin-based combination therapies (ACTs) constitute the principal strategy for combating malaria in contemporary times, and research into the multifaceted components of Artemisia annua L. ( A. annua ) has garnered widespread interest among scientists. The aim of this study was to prepare A. annua extracts (nACTs) and to explore whether nACTs have higher bioavailability and efficacy than artemisinin (ART) alone due to its multiple bioactive components. Initially, the in vivo antimalarial activity of nACTs was evaluated by two murine malaria models. The results revealed that the antimalarial effect of nACTs was about 10-fold higher than that of ART alone when administered at the same dosage of ART. Then, we analyzed the pharmacokinetic characteristics of nACTs in rat plasma. Remarkably, nACTs exhibited significantly enhanced oral bioavailability, longer half-life as well as extended mean retention time in rats. In addition, the impact of nACTs on P-glycoprotein (P-gp) was evaluated using the Caco-2 cell line. The results showed that both ART and nACTs reduced the efflux rate of the P-gp substrate rhodamine 123 (R123) and induced the expression of P-gp in Caco-2 cells over a range of concentrations. nACTs had certain components-deoxyartemisinin (DEART), artemisinic acid (AA), and dihydroartemisinic acid (DHAA)-that inhibited the efflux and translocation of P-gp and facilitated the reduction of ART efflux. In conclusion, A. annua extracts significantly improved the antimalarial efficacy and bioavailability compared with ART.
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FASEB JOURNAL
·
2025
·Vol. 39
·期 9
·e70561
·
IF 4.2
Haocong Zhang,Xianyun Qin,Hong Yuan,Liangbi Xiang,Hailong Yu
Neuronal inflammation and apoptosis aggravate the secondary injury after spinal cord injury (SCI). Cathepsin L (CTSL) is a lysosomal cysteine protease with effects on the regulation of inflammation, but its role in SCI remains unclear. The in vivo T10 mouse spinal cord contusion model was established. The results showed that CTSL expression was increased following SCI and then gradually decreased. Moreover, CTSL was mainly expressed in microglia. To detect the function of CTSL, after contusive injury, the mice were immediately injected with lentiviruses carrying CTSL shRNA. The results showed that CTSL depletion promoted functional recovery, accompanied by increased locomotor ability. CTSL deficiency reduced lesion cavity areas by inhibiting neuronal apoptosis and neuroinflammation. Indeed, CTSL deficiency decreased the secretion of TNF-α, IL-6, and MCP-1 and M1 microglia polarization in the spinal cord. CTSL depletion inhibited the expression and assembly of NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome. In vitro, CTSL expression was increased in LPS-treated BV2 cells. CTSL silencing repressed LPS-induced M1 polarization, as evidenced by the reduction in TNF-α, IL-6, and MCP-1 expression in the supernatant of BV2 cells. CTSL knockdown induced the downregulation of NLRP3 expression and activation. The inhibition role of CTSL knockdown in microglial inflammation and M1 polarization was reversed by NLRP3 agonist. Collectively, the study suggests that CTSL induces the microglia M1 polarization-mediated inflammation via promoting NLRP3 activation and thereby inhibits functional recovery after SCI. Graphical The inhibition of CTSL improves functional recovery and alleviates neuroinflammation through microglial polarization after spinal cord injury. Mechanically, CTSL knockdown inhibited NLRP3 expression and activation, which repressed the production of pro-inflammatory cytokines in M1 microglia, thereby inhibiting spinal cord injury-induced neuroinflammation.
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AQUACULTURE NUTRITION
·
2025
·Vol. 2025
·期 1
·5623889
·
IF 3.9
Xianglin Cao,Rongjie Yuan,Yi Guo,Mengtao Jia,Yinyin Wei,Jiameng zhou,Han Cui,Baohua Li,Jianjun Chen
L-Carnitine is widely recognized for its involvement in lipid metabolism, but its effects on muscle quality and gut health in carp have not been well studied. The research aimed to investigate how L-carnitine influences muscle quality and intestinal health in high-fat-fed carp. The study was separated into four groups that received either the standard diet, a high-fat diet (HFD), or a HFD supplemented with 500 mg/kg L-carnitine (LLC), or a HFD supplemented with 1000 mg/kg L-carnitine (HLC) for 56 days. L-Carnitine was found to significantly reduce blood lipid levels. In addition, L-carnitine increased the crude protein content and decreased the crude fat content of high-fat-fed carp muscle while improving muscle fiber morphology and muscle quality. L-Carnitine increased the expression of genes related to intestinal tight junction proteins ( claudin-2 , occludin , and zo-1 ), improved the expression of genes related to intestinal inflammation, and enhanced the physical barrier function and organization of the intestine. Analysis of intestinal flora and intestinal metabolites showed that L-carnitine increased the diversity of the intestinal flora, increased the abundance of Cetobacterium , and influenced intestinal levels of bile acids, arachidonic acid, and tryptophan-related metabolites. In conclusion, supplementation with 1000 mg/kg L-carnitine improved muscle quality and intestinal health significantly in high-fat-fed carp by regulating muscle nutrient metabolism and intestinal flora.
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FLAVOUR AND FRAGRANCE JOURNAL
·
2025
·
IF 2
Zhi Wu,Jiahui Zheng,Yang Xu,Dairong Wang,Qingbin Han,Ping Liu,Xiaorong Liu,Lanyue Zhang
Discomfort caused by inflammation leads to stress and anxiety in patients and seriously decreases the patients' quality of life. People prefer to use natural products instead of anti-inflammatory drugs because of their low toxicity and side effects. Studies have shown that Sphagnum palustre L. ( S. palustre ) can be used as medicinal plant, but few studies have focused on its anti-inflammatory effects. This study explored the mechanism of action of the ethanol extract of the peat moss plant S. palustre on lipopolysaccharide-induced inflammation in macrophage RAW264.7 cells. Components in S. palustre ethanol extracts (SPE) were identified by HPLC-MS, which mainly included 4-methoxybenzaldehyde, 4-methoxycinnamaldehyde and oleanolic acid. The effects of different concentrations (6.25–100 μg/mL) of SPE after 24 h administration were evaluated to establish a cellular inflammation model. Three biological replicates were performed based on each experiment, the MTT assay results showed that a low concentration of SPE promoted cell proliferation marked by Formazan. In a neutral red uptake assay, the SPE group was effectively inhibited the cell phagocytosis rate. With the increase of SPE concentration, intracellular ROS release decreased, which detected by DCFH-DA. Immunofluorescence assay result showed that SPE inhibited the release of reactive oxygen species from macrophages with fluorescent markers and DAPI. SPE inhibited the release of nitric oxide from macrophages as well. What's more, SPE significantly decreased the protein expression of interleukin (IL)-1, IL-6, and nuclear factor (NF)-κB according to enzyme-linked immunosorbent and immunocytochemical assays. SPE reduces inflammation in macrophage RAW264.7 cells and thus is a promising natural anti-inflammatory plant.
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Polymers
·
2020
·Vol. 12
·期 9
·2053
·
IF 3.426
Chen Qi,Wenke Yang,Fuyan He,Jinshui Yao
Eight kinds of chiral diacid monomers were prepared with amino acids with different side groups or configurations. Polyester-imides (PEIs) were synthesized from these diacid monomers and diphenol monomers through polycondensation reaction, and the performances and properties were compared with the chiral polyamide-imides (PAIs) previously synthesized by our work group. Their thermal properties were analyzed by thermo gravimetric analysis (TGA) and dynamic thermomechanical analysis (DMA), and it was found that the glass transition temperature (Tg) of PEI was mainly affected by the volume of side groups. Their degradability was studied through buffer degradation experiments, and the changes in their water contact angle, molecular weight, structure and appearance during the degradation process were characterized by contact angle tester, gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). With degradation, the hydrophilicity of PEI was improved, and when amino acids with larger side groups or D configuration were introduced into the backbone of PEI, the degradability decreased.Keywords:chiral amino acids;polyester-imides (PEIs);degradation;thermal property
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Yan Zhang,Hai-Yan Wang,Xi-Wen He,Wen-You Li,Yu-Kui Zhang
In this work, we innovatively synthesized homochiral fluorescence nano molecularly imprinted polymers (D-MIP) with dual affinity (metal ion affinity and homochiral affinity) for the specific separation and detection of L-penicillamine (L-PA), which is a core-shell structure with a SiO 2 -covered CDs core and an imprinted layer with L-PA cavities. A switch for fluorescence response was built by chelating grafted Cu 2+ , what's more, the imprinted L-PA was pre immobilized by Cu 2+ to form the directional imprinting with predetermined spatial structure. More importantly, the homochiral affinity of D-galactose in homochiral molecularly imprinted polymers (D-MIP) greatly enhanced the selective adsorption of L-PA, and D-MIP showed a high selectivity factor (α) of 3.45, which is 1.9 times that of the non-homochiral molecularly imprinted polymers (MIP). Meanwhile, D-MIP exhibited a high enantiomeric excess (ee) value of 56% for separation of racemic PA. Additionally, a high sensitive and selective method was established for the detection of L-PA.
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