MATERIALS & DESIGN
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2024
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IF 7.6
Chengjun Peng,Guichun Wang,Jiaxin Li,Yuxiao Wang,Zixuan Shu,Maomao Tang,Xiaodong Ma,Jian Guo,Shuangying Gui
Periodontitis is characterized by local inflammatory response and the resultant alveolar bone resorption. The elevated reactive oxygen species (ROS) level not only aggravates local inflammation but also prohibits osteogenesis. Hence, simultaneously scavenging ROS and triggering the activity of osteoblasts may produce synergism in periodontium regeneration. Herein, we developed an injectable hydrogel system, CC-B-CAPE@CY-NPs, for periodontitis treatment, in which caffeic acid phenethyl ester (CAPE), a naturally-occurring anti-inflammatory ingredient, was caged by aryl borate for ROS-responsive release. Meanwhile, the nanoparticles incorporating Chrysin (CY), another natural product with osteogenetic property, were also coated in the hydrogel. In vitro, the hydrogel was capable of scavenging the excessive ROS and modulating the polarization of M1 macrophages, highlighting its anti-inflammatory potency. In addition, its osteogenetic effect was verified by the increased ALP activity in the hydrogel-treated periodontal ligament stem cells (PDLSCs). Our studies revealed the anti-inflammatory stemmed from its ablation of NF-κB signaling, while the activation of Wnt/β-catenin cascade accounted for its osteogenetic potency. In a rat periodontitis model, the capability of reducing ROS levels, modulating macrophage polarization, and promoting osteogenesis was transformed into the recovery of destructed alveolar bone. All above, the hydrogel possesses great potential as a promising remedy for periodontitis.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhipeng Sun,Yilin Ding,Zetao Wang,Huitong Luo,Qi Feng,Xiaodong Cao
Bacterial infection and the resulting inflammatory cascade are key factors hindering diabetic ulcers healing. The essential prerequisites for promoting diabetic ulcer healing are to sequentially achieve rapid sterilization, prevent reinfection, and modulate the inflammatory response. Inspired by the size-dependent sequential toxin release behavior of “Malaysian exploding ant”, we develop a ROS-responsive “exploding hydrogel” (E-gel) with size-dependent sequential release effects, which sequentially performs photothermal sterilization, persistent antibacterial activity, and immune regulation. Specially, polyhexamethylene guanidine (PHMG, P) and mesoporous polydopamine nanoparticles loaded with α-lipoic acid (α-LA@MPDA NPs, LD) were encapsulated into sulfhydryl-modified hyaluronic acid (HA-SH) through disulfide bonds to form a PLD/E-gel. The high temperatures generated by the photothermal effects of MPDA can kill over 90 % of bacteria within 10 min. During the swelling period of PLD/E-gel, PHMG diffuses out and effectively prevents the wound from re-infection. Along with the degradation of PLD/E-gel, submicron-sized α-LA@MPDA NPs are released from the cross-linked network to scavenge excessive ROS and modulate the inflammatory cascade. Animal experiments shows that PLD/E-gel accelerates the healing of infected diabetic wounds in SD rats by rapidly killing bacteria, inhibiting bacterial recolonization, relieving wound inflammation, and facilitating collagen deposition. To sum up, PLD/E-gel has a great application potential for diabetic wound healing.
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Nano Research
·
2024
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IF 9.5
Wang Ding,Jiang Qi,Shen Ruoyu,Peng Lijun,Zhou Wentao,Meng Tingting,Hu Fuqiang,Wang Jianwei,Yuan Hong
The destruction of the intestinal barrier is likely to cause an increase in intestinal permeability and cause pathological damage. Numerous studies have demonstrated that intestinal barrier function plays an important role in the occurrence and development of inflammatory bowel disease (IBD). Oral administration is the most common route for intestinal diseases. In this study, a synergistic strategy is proposed for IBD management through active barrier repair combined with anti-inflammatory treatment, which can interrupt the pathological process of IBD, resulting in the significantly improved efficacy of existing treatments. Based on the specific pH values and high reactive oxygen species (ROS) levels in inflammatory sites of IBD, an orally administrated ROS-responsive drug delivery system targeting inflamed colon has been designed, and confirmed in vitro and in vivo . The antiinflammatory drug dexamethasone acetate (Dex) and the barrier function regulator LY294002 are delivered by the synthesized nanocarrier to treat IBD synergistically by inhibiting inflammation and actively repairing the intestinal barrier through tight junctions (TJs). The accumulation of nanocarriers in the inflamed colon and synergistic efficacy has been validated in mice with colitis. In brief, a drug delivery system and a therapeutic strategy for IBD are successfully developed.
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ADVANCED FUNCTIONAL MATERIALS
·
2024
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IF 18.5
Huan Liu,Sumei Qin,Hongyan Zhang,Zhongyin Chen,Yiwei Zhao,Jia Liu,Yan Deng,Miaodeng Liu,Wei Chen,Zheng Wang,Lin Wang
Diabetic foot ulcer typically undergoes a delayed wound-healing process owing to the disordered microenvironment, including persistent inflammation, long-term hypoxia, and excessive fibrosis, eventually leading to severe disabilities and other poor outcomes. However, current strategies cannot meet the dynamic demands during the wound-healing process. Herein, a reactive oxygen species (ROS)–responsive oxygen-generating microneedle platform, consisting of a sericin-based glucose-responsive hydrogel loaded with verteporfin, for effective diabetic wound healing is proposed. Under a highly oxidative microenvironment, the functionalized sericin protein depletes overproduced ROS to generate oxygen, which can not only alleviate the hypoxic microenvironment and promote angiogenesis by activating extracellular signal-regulated kinase and Heme Oxygenase-1 pathways but also decrease the expression of proinflammatory cytokines. Moreover, the hyperglycaemic condition triggers the gradual dissociation of this microneedle platform and promotes the release of verteporfin to enhance scarless re-epithelization via inhibiting the yes-associated protein signal. This versatile sericin microneedle system integrates advantageous features including inflammation alleviation, angiogenesis promotion, and scar inhibition capabilities, accelerates diabetic wound repair in vivo, and offers a novel approach for oxygen self-supply and wound microenvironment remodeling.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Zihang Liu,Fenbo Ma,Bo Wu,Jialin Yu,Xuan Huang,Kui Huang,Bin Tang,Guofeng Wu
Bone defects arising from trauma, tumors, or infections pose clinical challenges, primarily due to the limitations of current biofiller materials, which often fail to conform to irregular defect geometries and mitigate post-surgical complications such as inflammation and oxidative stress. To address these issues, we developed an injectable, photocurable composite hydrogel, GelMA-HA-PSO, which is composed of methacrylated gelatin (GelMA), hyaluronic acid (HA), and psoralen (PSO). A key highlight of our design is its minimally invasive potential, enabled by the hydrogel's exceptional injectability and in situ photo-crosslinkable properties. This unique combination allows the hydrogel to seamlessly adapt to complex and irregular bone defects during clinical procedures, offering a significant advantage over traditional materials. The injectable nature of GelMA-HA-PSO facilitates precise delivery to the defect site, while its photocuring capability ensures rapid and stable fixation in place, minimizing the need for extensive surgical intervention. Beyond its engineering advantages, the GelMA-HA-PSO hydrogel exhibits multifunctional therapeutic properties, including anti-inflammatory, antioxidant, and osteogenic effects. In vitro studies have demonstrated its biocompatibility and efficacy in reducing reactive oxygen species levels in MC3T3-E1 cells, as well as suppressing pro-inflammatory cytokine expression in RAW264.7 macrophages. Its osteogenic potential was further confirmed through enhanced alkaline phosphatase activity and upregulation of osteogenesis-related genes and proteins. Immunofluorescence analysis validated its capacity to modulate inflammatory markers and promote osteogenic differentiation. The incorporation of HA enhanced the hydrogel's storage modulus and swelling properties, while PSO facilitated sustained drug release and improved its bioactivity. Collectively, this study demonstrates the development and validation of a multifunctional GelMA/HA/PSO hydrogel that integrates biological (antioxidant, anti-inflammatory, osteogenic) and engineering (injectable, photo-crosslinkable) properties, with a particular emphasis on its minimally invasive potential for bone defect repair.
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PubMed
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ADVANCED FUNCTIONAL MATERIALS
·
2025
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IF 19
Junwei Su,Xianzhen Dong,Chao Xu,Zheng Wang,Changjiang Liu,Hongjun Yang,Dong Zhang,Aixi Yu
The multi-territory perforator flap is a widely used microsurgical technique for repairing skin and tissue defects in diabetes. In the diabetic microenvironment, oxidative stress and inflammation from reactive oxygen species (ROS) lead to compromised blood supply to the flap, resulting in challenges for survival. The common complication of multi-territory perforator flap is distal necrosis, which is primarily attributed to the Choke zone, the critical location characterized by delayed blood supply and inadequate neovascularization. To address this issue, a ROS-responsive MSL@Z/G hydrogel is developed by encapsulating metformin-Sr-L-Arg@ZIF-90 (MSL@Z) nanoparticles into gelatine methacrylamide (GelMA), enabling the release of metformin, Sr ions and NO. The enhanced deformation resistance and compressive strength properties of the MSL@Z/G hydrogel make it suitable for tissue reconstruction and drug delivery. Additionally, the MSL@Z/G hydrogel exhibits antioxidant and anti-inflammatory effects, thereby modulating the vascular microenvironment. In the dorsal multi-territory perforated flap model of type 2 diabetic rats, the MSL@Z/G hydrogel demonstrates the ability to alleviate inflammation and promote neovascularization of the Choke zone, reducing distal necrosis, which holds great promise for improving flap survival in diabetes.
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JOURNAL OF INORGANIC BIOCHEMISTRY
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2025
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IF 3.2
Meixuan Zhang,Qin Zhang,Xuemin Mu,Xiaolin Wang,Shuhui Zhang,Wenyi Zhao,Xiaoming Song,Lulu Pan,Yanyan Li
The escalating threat of bacterial infections underscores the pressing need for innovative antibacterial solutions. In this study, BA-BBR@MPN nanoparticles (BA-BBR@MPN NPs) were synthesized through the self-assembly of berberine (BBR) and baicalin (BA), followed by modification with a metal-phenolic network (MPN) derived from caffeic acid and Fe 3+ . In the slightly acidic environment of the infected site, the MPN decomposes to release iron ions, BBR, and BA. The iron ions catalyze the generation of hydroxyl radicals (·OH) from hydrogen peroxide (H 2 O 2 ), enabling chemodynamic therapy (CDT) while simultaneously depleting glutathione levels. When combined with 808 nm laser-induced photothermal therapy (PTT), the system demonstrates synergistic antibacterial effects. Both in vitro and in vivo studies confirmed the platform's broad-spectrum antibacterial activity against Gram-positive and Gram-negative bacteria, along with excellent biocompatibility and significant wound-healing promotion. This multifunctional nanoplatform represents a promising strategy for combating bacterial infections while simultaneously promoting tissue repair.
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DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
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IF 5.4
Yuxiang Wang,Hongyu Liu,Beizhang Zhang,Guannan Zhang,Hailong Hao
Sonodynamic therapy (SDT) is considered an effective method for treating bladder cancer, but the limited electron transfer of a single material and the hypoxic microenvironment of cancer limit the efficiency of SDT. Here, barium titanate (BTO) was prepared, and Cu was doped into BTO through hydrothermal synthesis and high-temperature annealing, followed by surface conjugation of tumor-specific proteins (BTO/Cu/PDA/Nectin-4). The Cu doping enhances the ferroelectric polarization of BTO and reduces its bandgap, facilitating electron transfer. Meanwhile, Cu can react with hydrogen peroxide (H 2 O 2 ) via the Fenton reaction to generate reactive oxygen species (ROS), thereby enhancing the chemodynamic therapy (CDT) effect. The combination of SDT and CDT enhanced ROS production, and both in vitro and in vivo results demonstrated excellent cytotoxic effects and tumor suppression against bladder cancer cells. This combined SDT and CDT approach offers an effective therapeutic strategy for antitumor treatment.
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JOURNAL OF FISH DISEASES
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2025
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IF 2.2
Bo Li,Penghui Yu,Lei Su,Jianguo Su,Yu Gao,Gailing Yuan
Aeromonas hydrophila- induced bacterial sepsis is a major threat to aquaculture, characterised by excessive inflammation, ferroptosis (an iron-dependent lipid peroxidation-driven cell death) and oxidative damage, which collectively lead to high mortality. Taurine (TAU) and glutathione (GSH) have been demonstrated to have potential therapeutic efficacy against ferroptosis-associated pathologies through redox modulation. However, their combined effects and underlying mechanisms in alleviating A. hydrophila infection remain unclear. In this study, we investigated the protective roles of TAU, GSH and their combination in vitro using yellow catfish ( Pelteobagrus fulvidraco ) macrophages and grass carp ( Ctenopharyngodon idella ) kidney (CIK) cells and in vivo using yellow catfish challenged with A. hydrophila . The results showed that TAU and GSH, either alone or in combination, alleviated oxidative stress by significantly reducing reactive oxygen species (ROS) accumulation and inhibited nuclear factor-κB (NF-κB) pathway activation. This led to downregulated expression of pro-inflammatory cytokines ( IL- 1β and TNF -α) and upregulated anti-inflammatory cytokines ( IL- 10 and TGF -β), accompanied by alleviated ferroptosis. In vivo, dietary supplementation with TAU (10 g/kg) or GSH (350 mg/kg) alone improved survival rates (51.7% and 38.3%, respectively), reduced tissue bacterial loads and protected intestinal and hepatic tissues by preserving mucosal barrier function. However, the TAU + GSH combination unexpectedly decreased survival (28.3%) due to excessive immunosuppression (overproduction of IL- 10 and TGF -β) and impaired mucosal barrier, which exacerbated pathogen colonisation. Together, our findings demonstrate that TAU and GSH alleviate A. hydrophila -induced oxidative stress by reducing mitochondrial ROS overproduction and disrupting the ROS/NF-κB signalling pathway, thereby attenuating inflammatory cytokine storms and ferroptosis. These results provide novel insights into the pathological mechanisms of bacterial sepsis in fish and develop sustainable strategies to improve disease resistance in aquaculture.
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PubMed
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Food Bioscience
·
2025
·
IF 5.9
Yun-Lu Song,Jia-Lin Xing,Charles Ampomah-Dwamena,Shuai-Hui Ma,Mao-Song Pei,Han-Li Liu,Rui-Ping Feng,Yong-Ke Wu,Tong-Lu Wei,Hai-Nan Liu,Da-Long Guo
Grape seed extract (GSE) is a natural polyphenolic compound with potent antioxidant activity. We developed a composite preservation coating material by combining GSE with nano-zinc oxide (Nano-ZnO) and chitosan (CHI), two widely used agents in postharvest preservation. The CHI-GSE-Nano-ZnO coating effectively inhibited postharvest decay and berry abscission while delaying quality deterioration in grapes. Treatment with the composite coating enhanced the activities of antioxidant enzymes (SOD, POD, CAT, and APX) while reducing oxidative stress markers (O 2 - , H 2 O 2 , and malondialdehyde), thereby mitigating ROS accumulation to preserve postharvest grape quality. To elucidate the underlying mechanism, transcriptome sequencing was conducted on ‘Kyoho’ grape samples collected 3, 6, and 9 days post-treatment with CHI, CHI-GSE, and CHI-GSE-Nano-ZnO. Transcriptomic profiling revealed upregulation of VvSTS484 , a pivotal stilbene synthase (STS)-encoding gene, in the CHI-GSE-Nano-ZnO group. Promoter analysis identified multiple MYB-binding cis-elements in VvSTS484 . Yeast one-hybrid and transient dual-luciferase assays demonstrated that the transcription factor VvMYB714 directly binds to four specific MBS motifs in the VvSTS484 promoter (MBS1, -410 to -416 bp; MBS2, -1201 to -1207 bp; MBS3, -1554 to-1560 bp; and MBS4, -1906 to -1912 bp). This molecular interaction activated VvSTS484 expression, leading to enhanced resveratrol biosynthesis that peaked at day 9 post-treatment. The elevated resveratrol level in the CHI-GSE-Nano-ZnO group aligned with reduced oxidative stress and delayed quality deterioration, consistent with its established role in enhancing plant stress tolerance. In conclusion, the CH-GSE-Nano-ZnO composite coating inhibits postharvest decay via the MYB- pSTS module, providing a theoretical basis for developing natural product-based preservation technologies for fruit and vegetables.
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npj Regenerative Medicine
·
2022
·Vol. 7
·期 1
·1-15
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IF 14.404
Wu Jianjun,Qin Zainen,Jiang Xianfang,Fang Depeng,Lu Zhenhui,Zheng Li,Zhao Jinmin
Excessive reactive oxygen species (ROS) are one of the leading mechanisms in the initiation and development of osteoarthritis (OA). However, conventional injection of ROS-responsive drug delivery systems (DDSs) such as nanoparticles and hydrogels usually cannot provide effective treatment due to rapid clearance and degradation or low bioavailability. In this study, a ROS-responsive nanofiber membrane named PLA/PEGDA-EDT@rGO-Fucoxanthin (PPGF) is fabricated by electrospinning, wherein PEGDA-EDT served as the ROS-responsive motif, reduced graphene oxide (rGO) as the drug carrier and fucoxanthin (Fx) as the antioxidative and anti-inflammatory agent. The results demonstrated that the PPGF nanofiber membrane exhibited sustained and long-term Fx release behavior (at least 66 days) in response to hydrogen peroxide (H 2 O 2 ) in vitro. With low cytotoxicity and smart ROS responsiveness, PPGF showed excellent anti-inflammatory and antioxidative effects on IL-1β-induced chondrocytes by potent ROS scavenging potential and upregulation of antioxidative enzymes. It also demonstrated the attenuation of OA progression with the reduced Osteoarthritis Research Society International (OARSI) score by 93.17% in 8 weeks. The smart ROS-responsive, biodegradable and biocompatible nanofiber membranes possess great potential for OA therapy under arthroscopy.
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PubMed
高影响因子
INTERNATIONAL IMMUNOPHARMACOLOGY
·
2022
·
IF 5.714
Liping Wang,Tiantian Ji,Ye Yuan,Haoyu Fu,Yang Wang,Shaobo Tian,Jia Hu,Lin Wang,Zheng Wang
The dramatically increasing incidence and prevalence of inflammatory bowel disease (IBD) are reportedly related to a Western diet, which is characterized by high sugar consumption. Dietary simple sugars aggravate IBD in animal models. However, the mechanisms underlying this effect remain unclear. Given that high-fructose corn syrup (HFCS) is a major added sugar in food and beverages, we focus on HFCS and investigated the effects of HFCS on a dextran sulfate sodium (DSS)-induced murine colitis model and in RAW264.7 macrophages. Our data demonstrate that short-term consumption of HFCS aggravates colitis and upregulates the proportion of macrophages in IBD mice but not in healthy mice. We find that HFCS promotes proinflammatory cytokine production through reactive oxygen species (ROS)-mediated nuclear factor-κB (NF-κB) signaling in RAW264.7 macrophages. Furthermore, N-acetylcysteine (NAC), an ROS scavenger, alleviates HFCS-aggravated colitis in mice and inhibits the ROS-mediated NF-κB signaling pathway in RAW264.7 macrophages. Our work unveils the important role of macrophages in HFCS-induced exacerbation of colitis and reveals the mechanism of how HFCS immunologically aggravates IBD.
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PubMed
高影响因子
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