Foods
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2026
·Vol. 15
·期 10
·1732
·
IF 6
Kaiyin Hu,Jinxin Liu,You Su,Yijun Wang,Huimin Guo,Xiaochun Wan,Zhongwen Xie,Li Sun
Green tea is highly popular due to its richness in polyphenols exhibiting broad bioactivities. Tea polyphenols, primarily catechins and flavonoids, demonstrate health benefits following biotransformation by the gut microbiota to overcome limited bioavailability. However, metabolites and interaction between green tea polyphenol and the gut microbiota remains to be fully elucidated. This study investigates the biotransformation of metabolites and interaction between human gut microbiota (HGM) and green tea extract (GTE) through in vitro anaerobic fermentation. Temporal bioactivity assessments demonstrated that fermentation-enhanced antioxidant capacity and inhibition potential ofα-glucosidase,α-amylase and pancreatic lipase peak at 6 h, showing strong correlations with polyphenol and flavonoid biotransformation kinetics. Using the untargeted metabolomics approach, 55 characteristic differential compounds during the fermentation process in GTE were characterized, including 15 catechins, 29 flavonoids, five organic acids and six other phytochemicals. Furthermore, nine microbial-transformed metabolites derived from GTE flavonoids were identified and the corresponding metabolic pathways were proposed simultaneously. Analysis of 16S rRNA gene sequencing revealed that GTE significantly enhanced gut microbiota diversity and induced structural reorganization, specifically enriching genera such asBacteroides,Bifidobacterium,LactococcusandEnterococcus, which are likely involved in flavonoid biotransformation of GTE. Thus, the findings provide new insights for elucidating microbiota-mediated metabolites of green tea polyphenol, and their bidirectional interactions in the human gut.
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Catalysts
·
2026
·Vol. 16
·期 5
·420
·
IF 4.5
Shijun Zhu,Xinchen Zhang,Shangming Shen,Yang Wang,Yongshu Hu,Hao Yang,Wenbin Liu,Xiaoyan Ma,Jing Deng
Biochar (BC)-activated peracetic acid (PAA)-based advanced oxidation processes (AOPs) were increasingly considered as cost-efficient and eco-friendly water treatment technologies for the removal of organic pollutants. However, the specific role of intrinsic carbon, nitrogen species and structure properties played in activation mechanism is still vague. In this study, the waste tea residues-based biochar (WTBC) was prepared by thermal carbonization and applied to activate PAA for the degradation of bisphenol A (BPA). The product carbonized at 800 °C (WTBC800) possessed larger specific surface area (342.57 m2/g), more abundant porous structure and massive defects state (ID/IG= 3.53), and exhibited a superior activation performance with 83.7% BPA removal within 120 min. Adsorption and non-radical oxidation pathways [e.g., the mediated electron transfer process (ETP) and singlet oxygen (1O2) generation] were evidenced to play the dominant roles in the BPA degradation through the formation of metastable complex WTBC-PAA*. The graphitic carbon, functional nitrogen species, defects structure and persistent free radicals (PFRs) in WTBC were proposed to contribute to the activation of PAA. Overall, relatively higher dosages of WTBC (0–0.5 g/L) and PAA (0–1.5 mM) facilitated the BPA degradation. The solution pH and water matrix (e.g., Cl−, NO3−, HCO3−and SO42−) presented a negligible effect on the BPA degradation in WTBC/PAA system. This study not only proposes a sustainable approach for organic pollutants removal in wastewater, but also promotes the resource re-utilization of agricultural waste.
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Journal of Materials Research and Technology-JMR&T
·
2026
·
IF 7.3
P.Y. Guo,Q.H. Wu,X.J. Wang,M.X. Wei,Y. Shao,Z.G. Liu
Due to the combined effects of high-density electric fields, water vapor, and residues, adjacent solder joints are prone to electrochemical migration. This study investigates the electrochemical reaction processes and mechanisms of Sn and Sn-58Bi in methanesulfonic acid(MSA), sulfosalicylic acid(SSA), diethanolamine(DEA), triethanolamine(TEA), and triisopropanolamine (TIPA) flux solutions. The results show that under natural corrosion conditions Sn-58Bi exhibits better electrochemical corrosion resistance than Sn. Under dynamic potential conditions, reaction rates of Sn-58Bi exceed that of Sn due to the dissolution of Bi. The dissolution threshold for Bi occurs at approximately 0.15 V in the TEA solution, 0.37 V in the TIPA solution, and 1 V in the DEA solution. Sn-58Bi in the MSA and SSA solutions primarily undergoes Sn dissolution, whereas in the DEA, TEA, and TIPA solutions, Bi dissolution is the dominant process. Under a constant voltage of 1 V vs.SCE , the dissolution current of Sn is lower than that of Sn-58Bi. Sn-58Bi in the TIPA solution shows instability in the passivation film. Under a constant voltage of 5 V vs.SCE , the stable current values for both solders are similar.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Fangliang Li,Tongjie Luo,Jiale Hou,Tao Fei,Jiachao Zhang,Lu Wang
Raspberry leaf as a tea substitute has been consumed in China for long time. In this work, the inhibitory activities of raspberry leaf-tea (RLT) extract on α -glucosidase ( α -GLU)/ α -amylase ( α -AMY) were measured, and the effects of the RLT extract on the glucose consumption of 3T-3L1 cells were also investigated. Results confirmed that RLT 70% ethanol extract (REE) exhibited strong inhibitory activities on α -GLU/ α -AMY in vitro . In addition, REE remarkably improved the glucose consumption of 3T-3L1 cells. Subsequently, eight major inhibitors including epigallocatechin gallate (EGCG), delphinidin-3- O -glucoside, cyanidin-3-rutinoside, isoorientin, procyanidin C3, dihydromyricetin, rutin, and isovitexin were screened and identified through affinity ultrafiltration combined with HPLC-MS/MS. Confirmation tests verified that these major compounds were responsible the inhibitory activities of the REE on α -GLU/ α -AMY. Molecular docking studies revealed that these inhibitors can well interact with α -GLU/ α -AMY via van der Waals force or hydrogen bonding, thus inhibiting their activities. In a short, raspberry leaf can be exploited as a promising anti-hypoglycemic material. This study provides a theoretical basis for discovering active components from medicinal plants using affinity ultra-filtration with multi-targets.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Yue-Yue Chang,Hai-Long Wu,Tong Wang,Huan Fang,Xiao-Zhi Wang,Sengsavanh Khanthavong,Phimphone Keobountham,Ru-Qin Yu
As the China’s top-ranked oolong tea, there are many varieties of Wuyi rock tea (WRT) in the market. The prices of different varieties vary greatly, and the differential chemical composition is unclear. Therefore, we developed a rapid and systematic chemometrics-assisted HPLC–DAD strategy for targeted metabolites analysis and variety discrimination of WRT. The strategy involved design of experiment , targeted metabolites quantification, varieties discrimination and differential component screening. Specifically, optimal extraction conditions of WRT are obtained by response surface methodology-Box-Behnken design method. Then, 22 targeted metabolites are rapidly and accurately quantified by alternate trilinear decomposition algorithm (ATLD) with average spiked recoveries ranging from 85.4% to 108.6%. Finally, the four WRT varieties (110 samples) are discriminated by linear discriminant analysis (LDA) and partial least squares-discriminant analysis (PLS-DA) with relative concentration results as model input. The correct classification rates obtained by PLS-DA are greater than 88%, especially for high-quality “Niulankeng rougui” tea, its specificity and sensitivity are 100%. Moreover, ten differential metabolites related to identification are screened by variable importance in projection (VIP). This work scientifically and comprehensively elaborates the whole-process of WRT analysis, which can become a comprehensive food analysis strategy for the purpose of precise quantification and pattern recognition.
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Xiao-Hua Zhang,Hui-Na Cui,Jing-Jing Zheng,Xiang-Dong Qing,Kai-Long Yang,Ya-Qian Zhang,Lu-Meng Ren,Le-Yuan Pan,Xiao-Li Yin
The flavor and aroma quality of green tea are closely related to the harvest season. The aim of this study was to identify the harvesting season of green tea by alcohol/salt-based aqueous two-phase system (ATPS) combined with chemometric analysis. In this paper, the single factor experiments (SFM) and response surface methodology (RSM) optimization were designed to investigate and select the optimal ATPS. A total of 180 green tea samples were studied in this work, including 86 spring tea and 94 autumn tea. After the active components in green tea samples were extracted by the optimal ethanol/(NH 4 ) 2 SO 4 ATPS, the qualitative and quantitative analysis was realized based on HPLC-DAD combined with alternating trilinear decomposition-assisted multivariate curve resolution (ATLD-MCR) algorithm, with satisfactory spiked recoveries (86.00 %–112.45 %). The quantitative results obtained from ATLD-MCR model were subjected to chemometric pattern recognition analysis. The constructed partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) models showed better results than the principal component analysis (PCA) model, and the R 2 X cum values (>0.835) and R 2 Y cum (>0.937) were close to 1, the Q 2 cum values were greater than 0.75 (>0.933), and the differences between R 2 Y cum and Q 2 cum were not larger than 0.2, indicating excellent cross-validation prediction performance of the models. Furthermore, the classification results based on the hierarchical clustering analysis (HCA) were consistent with the PCA, PLS-DA and OPLS-DA results, establishing a good correlation between tea active components and the harvesting seasons of green tea. Overall, the combination of ATPS and chemometric methods is accurate, sensitive, fast and reliable for the qualitative and quantitative determination of tea active components, providing guidance for the quality control of green tea.
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Journal of Environmental Chemical Engineering
·
2022
·
IF 7.968
Zibin Pan,Zhengxian Chen,Gary Owens,Zuliang Chen
Biosynthesis strategies are promising methods for the sustainable preparation of nanoparticles because they are less toxic to living organisms and the environment. However, there are still several knowledge gaps concerning the exact mechanisms involved in the biosynthesis of bimetallic composites when used for the removal of arsenic species. Thus, this work focused on the biosynthesis mechanism involved in the formation of a Fe/Cu bimetallic bio-composite (Fe/Cu-OC), and determination of the specific biomolecules in tea extract involved in biosynthesis, and identification of the reactive sites involved in the removal of As(V). FTIR , XPS, GC-MS, and LC-MS analysis of the main components of the tea extract proved that the surface of Fe/Cu-OC contained many different functional groups, including -OH, Fe/Cu-O, and O C-OH, which were mainly associated with theanine and caffeine complexes. SEM and TEM analyses indicated that Fe/Cu-OC had both crystalline and amorphous features with an average particle size of 366 ± 10 nm. A 97 % removal efficiency for As(V) was obtained by Fe/Cu-OC, suggesting that the surface contained numerous reactive sites, including unsaturated Fe and Cu complexes, as well as saturated benzene rings. Moreover, DFT calculations indicated that the Gibbs free energy changes for the preparation of Fe/Cu-OC were < 0, suggesting that in the composites biosynthesis was a thermodynamically favored spontaneous reaction. A biosynthetic mechanism for the formation of Fe/Cu-OC was proposed, involving a series of spontaneous coordination, redox, and organic adsorption reactions.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Zeming Zhu,Jianfeng Hu,Zicheng Zhong
Encapsulation technology can increase the stability and maintain the volatile active substances of plant essential oils. In the present study, tree essential oil (TTO) was encapsulated with polylactic acid (PLA) modified by octenyl succinic anhydride chitosan (OSA-CS) as shell materials to form long-term antibacterial and pH-responsive microcapsules. The PLA/ [email protected] microcapsules were characterized by high performance liquid chromatography (HPLC), scanning electron microscopy (SEM) and antibacterial performance testing. The results showed that the average particle size of microcapsules was 10 μm, and the encapsulation efficiency and drug loading efficiency of TTO reached 81.5 % and 60.3 %. After 4800 min of release in media at different pH (5 and 7) still sequestered 55.32 % and 56.74 % of TTO which approved the shell of microcapsules responded to different pH values. The microcapsules remained stable for 80 days after drying, and preserving 39.7 % of the core material. The morphology of PLA/ [email protected] microcapsules revealed that the PLA/ [email protected] microcapsules presented smooth and firm structure. Antibacterial test for staphylococcus aureus of those microcapsules implied that the bacteriostatic rate reached 100 % after 72 h. Bio-based macromolecular modification strategies can provide inspiration for the development of green microcapsules.
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Food Packaging and Shelf Life
·
2022
·
IF 8.749
Xingzi Lan,Tingting Luo,Zhixin Zhong,Dongchao Huang,Chengfeng Liang,Yurong Liu,Han Wang,Yadong Tang
Gelatin nanofibrous membrane has attracted increasing attention as biodegradable and edible food packaging. However, the lack of bioactivity and the weak water resistance of gelatin nanofibrous membrane severely limits its application in food preservation. Therefore, in this study, antioxidant agent tea polyphenol (TP) and antibacterial agent ε-poly ( L -lysine) (ε-PL) were incorporated into gelatin nanofibrous membrane. To enhance the water resistance, the TP and ε-PL loaded gelatin nanofibrous membrane was cross-linked at high temperature. And the antibacterial activity and antioxidant activity of gelatin/TP/ε-PL nanofibrous membrane after cross-linking were investigated. The results showed that the gelatin/TP/ε-PL nanofibrous membrane exhibited homogenous bead-free structure. The UV–vis spectra confirmed the successful incorporation of TP and ε-PL into nanofibers . The water contact angle of the gelatin/TP/ε-PL nanofibrous membrane after cross-linking was increased. The fabricated nanofibrous membrane could prevent microbial penetration. And the nanofibrous membrane still exhibited excellent antibacterial activity and antioxidant activity after cross-linking. Finally, the MTT assay demonstrated the biocompatibility of nanofibrous membrane. The above results suggested that the prepared gelatin/TP/ε-PL nanofibrous membrane exhibited great potential in bioactive food packaging.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Gangfeng Li,Shaobiao Feng,Lili Yan,Lijuan Yang,Bei Huo,Liuliu Wang,Sheng Luo,Dexin Yang
Due to a small amount of Cu (Ⅱ) ions being beneficial and too much being harmful, it is necessary to establish a rapid and direct detection method. Herein, we reported a platform based on multiwalled carbon nanotubes (MWCNTs), 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF6), and Nafion solution-modified glassy carbon electrode (GCE) for the direct electrochemical detection of Cu (II) ions. We used differential pulse anodic stripping voltammetry, including the electrodeposition of Cu (Ⅱ) ions on the modified GCE and subsequent anodic stripping. Under the optimum conditions, the linear range was 20 μg·L −1 ∼ 950 μg·L −1 , the limit of detection (LOD) was 16 μg·L −1 , and the limit of quantification (LOQ) was 54 μg·L −1 for Cu (II). We realized the quantitative detection of Cu (Ⅱ) ions in juice and tea beverage without tedious pretreatment. The result showed that the sensor had good anti-interference and practicability for actual food samples.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Chunfeng Chen,Shenqun Wu,Yuren Li,Youyi Huang,Xiaoping Yang
This study intends to produce a black tea vinegar (BTV) from low-quality bulk black tea in order to increase its consumption and added value, and to benefit from its functional characteristics. Three strains ( Acetobacter sp. GDMCC1.152, Acetobacter pasteurianus GDMCC1.67, and Acetobacter aceti ATCC15973) of acetic acid bacteria (AAB) were inoculated into black tea infusion (BTI) with ethanol to produce BTV, and their effects on bioactive compounds, antioxidant activity, and volatile compounds in the vinegars were revealed. The results showed that the three strains were suitable for the production of BTV, and the vinegar quality was the best after acetic acid fermentation for 6 days. Compared with BTI, the contents of organic acids and volatile compounds and the antioxidant activity of the vinegars increased significantly. Among them, the vinegar produced by GDMCC1.152 strain had the highest content of organic acids, tea polyphenols (TPs), thearubigins (TRs), theabrownines (TBs), and volatile compounds. It also possessed the strongest antioxidant activity and a rich taste with an apple-like aroma. In summary, AAB fermentation can effectively improve the flavor and quality of BTI and produce a new healthy product with high activity, and Acetobacter sp. GDMCC1.152 is the most suitable strain for the production of BTV.
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Food & Function
·
2022
·Vol. 13
·期 18
·9299-9310
·
IF 6.317
Jia-Ping Ke,Jing-Ya Yu,Biao Gao,Feng-Lin Hu,Feng-Qing Xu,Guangmin Yao,Guan-Hu Bao
Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the antiaging properties of green tea is limited to the major catechin epigallocatechin gallate (EGCG). The search for new ingredients in tea with strong antiaging activity deserves further study. Here we isolated and identified two new catechins from Zijuan green tea, named zijuanin E (1) and zijuanin F (2). Their structures were identified by extensive high-resolution mass spectroscopy (HR-MS), nuclear magnetic resonance (NMR), ultraviolet–vis (UV), infrared (IR) and circular dichroism (CD) spectroscopic analyses, and their 13C NMR and CD data were calculated. We used the nematode Caenorhabditis elegans (C. elegans) to analyze the health benefits and longevity effects of 1 and 2. Compounds 1 and 2 (100 μM) remarkably prolonged the lifespan of C. elegans by 67.2% and 56.0%, respectively, delaying the age-related decline of phenotypes, enhancing stress resistance, and reducing ROS and lipid accumulation. Furthermore, 1 and 2 did not affect the lifespan of daf-16, daf-2, sir-2.1, and skn-1 mutant worms, suggesting that they might work via the insulin/IGF and SKN-1/Nrf2 signaling pathways. Meanwhile, 1 and 2 also exhibited strong antioxidant activity in vitro. Surface plasmon resonance (SPR) evidence suggests that zijuanins E and F have strong human serum albumin (HSA) binding ability. Together, zijuanins E and F represent a new valuable class of tea components that promote healthspan and could be developed as potential dietary therapies against aging.
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危险品化学品经营许可证（带存储）