MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Xintong Li,Jiale Cui,Jin Li,Yulian Wang,Guoqing Shi
Gastrin 17 (G-17) is a major biomarker for the diagnosis of gastric cancer. As the serum concentrations of gastrin are low, highly sensitive detection methods are needed. Previous studies have shown that oriented immobilization of detection antibodies can improve the sensitivity of lateral flow immunoassay (LFIA) methods. Herein, the antibody binding capacity of four Fc-specific peptides reported in different studies was evaluated, and two peptides with better binding potency were further modified with cysteine or the peptide GAC at their C-terminus. The peptide FYWHCLDEGAC exhibited the highest antibody binding capacity and was applied to directly immobilize the anti-G-17 antibody on microspheres with carboxyl-functionalized surfaces and europium-based time-resolved fluorescence. An LFIA method was developed to detect G-17 in human serum, and the limit of detection (LOD) of this method for G-17 was 0.08 pmol L -1 , which was 10 times less than that of the nonoriented immobilization method. The working range of this method is 0.27 ∼ 600 pmol L -1 , with a recovery rate of 85.2 % −106.7 % and a CV value less than 12.4 %. This method is easy to use, and the total detection process takes less than 20 min. The test results for the actual serum samples were consistent with the commercial kit results (R 2 > 0.95), demonstrating that the method can be utilized for point-of-care testing of G-17. This approach to orientedly immobilize antibodies on nanomaterials can be used to develop sensitive LFIA methods for various molecules.
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Journal of Energy Storage
·
2024
·
IF 8.9
Yingjie Zhang,Yuzheng Li,Chengxiao Xu,Daming Li,Jun Cao,Peipei Huo
Lithium‑sulfur batteries (LSBs) are recognized as promising candidates for the next generation of secondary batteries due to their high energy density, high theoretical capacity, and low environmental impact. However, the poor electrical conductivity of sulfur and Li 2 S, the “shuttle effect” of polysulfides, and the slow kinetics of redox reactions have significantly hindered the practical application of LSBs. In this work, CoSe 2 -loaded carbon nanofibers (CoSe 2 @CNF) derived from metal-organic frameworks were successfully prepared by electrospinning and used as a functional coating on the polypropylene separator. The three-dimensional network structure of CNFs not only improves the electrical conductivity of the material but also provides channels to facilitate ion and electron transport, while the CoSe 2 particles distributed on the surface effectively act as adsorption and catalytic active sites for lithium polysulfides. Under the synergistic effect, the CoSe 2 @CNF-modified separator suppressed the shuttle effect and significantly enhanced the kinetics of redox reactions. The LSBs assembled with CoSe 2 @CNF-modified separator exhibited excellent electrochemical performance, achieving an initial capacity of 1445.6 mAh g −1 at 0.5C and maintaining a discharge specific capacity of 849.5 mAh g −1 after 275 cycles.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xinnan Ma,Juan Han,Jiacong Wu,Yang Zhou,Chunmei Li,Lei Wang,Yang Lu,Yun Wang
Efficiently enhancing hydrogen peroxide (H 2 O 2 ) production via photocatalysis remains challenging. This study employed genetic engineering to express His-tagged superoxide dismutase (His-SOD), which was selectively anchored onto the coordinatively unsaturated metal sites of etched UIO-66-NH₂ (UIO-66-NH 2 etch ) through coordination-driven assembly. The synergistic effect between His-SOD and UIO-66-NH 2 etch significantly enhanced both the stability and substrate affinity of the enzyme, while simultaneously boosting the photocatalytic activity of the MOF. Compared to its free counterpart, the immobilized His-SOD exhibits a 62 % increase in catalytic efficiency and a more than threefold extension in thermal deactivation half-life. The fabricated UIO-66-NH 2 etch @His-SOD composite achieved an impressive H 2 O 2 production rate of 3890.37 μmol·g −1 ·h −1 , exceeding that of the pristine UIO-66-NH 2 by over 2.5 times within 1 h and maintaining a 1.7-fold enhancement after 4 h. Owing to SOD's inherent superior antioxidative capabilities, the composite material demonstrates exceptional recyclability, retaining 95 % of its initial catalytic activity after four consecutive cycles. This study achieves specific immobilization of functionalized fusion proteins onto photocatalysts, and represents the first introduction of SOD into photocatalytic H 2 O 2 synthesis. This advancement provides valuable insights for the future integration of biocatalysts and synthetic photocatalysts, opening new avenue for research on photo-enzyme coupled catalysis.
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ENVIRONMENTAL RESEARCH
·
2025
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IF 7.7
Pan Tan,Jiaqin Wei,Sihong Luo,Guilin Wei,Xiaoan Li,Lijing Huang,Xirui Lu,Xiaoyan Shu
Glass matrices with high waste load capacity, stability, and economic viability holds significant technological implications for efficient and safe nuclear waste immobilization. Aluminosilicate glasses with various Si/Al molar ratios were successfully prepared by incorporating Al 2 O 3 into natural granite, effectively enabling precise regulation of the Si/Al ratios and overcoming the challenge of achieving complete vitrification. Here, we report a glass waste form with ultra-high CeO 2 loading (up to 20 wt .%) that exhibits excellent structural stability. At a Si/Al molar ratio of 3:1, cerium can incorporate into the glass network by forming Ce-O bonds through coordination with oxygen atoms in the [AlO 6 ] units. [AlO 6 ] is converted to more stable [AlO 4 ], thereby enhancing the stability of the glass. Up to 20 wt .% CeO 2 (9.62 mol%) can be fully incorporated into the glass system, where approximately 79.71 % of the bridging oxygen atoms form stable Si-O-Si and [AlO 4 ] units, contributing to a structurally robust glass network. The Ce-O bond length in the glass matrix is shorter (2.27 Å) than in CeO 2 , confirming enhanced coordination stability and aligning with superior chemical durability and mechanical properties. This work highlights the critical role of the Si/Al molar ratio and establishes low-cost granite could serve as a viable host material for nuclear waste immobilization.
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CHEMICAL GEOLOGY
·
2025
·
IF 3.6
Changjin Liang,Honghai Wu,Yaowei Li,Yufeng Guan
Ferrihydrite (Fh) can usually transform into phases with higher crystallinities. This may greatly alter Fh adsorption capacity for chromium ions, thereby affecting their immobilizations and fates. Foreign elements [e.g., lanthanum (La)] as impurities can become incorporated into Fh. Herein, the effect of La-substitution into Fh and the influence of the presence of Cr(VI) on Fh phase transformation and fate of Cr(VI) were investigated. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Electro-chemical impedance spectroscopy (EIS), and X-ray photoelectron spectroscopy (XPS) and other test methods were employed to probe phase changes after the Fe(II)-induced La-substituted Fh (La-Fh) transformations. At pH 5.5, no neoformed phase was observed until 7 d for La-Fh or pure Fh at 1 mM Fe(II) aq, but all La-Fh systems underwent transformations at 5 mM Fe(II)aq. A low pH and Fe(II)aq level favored lepidocrocite (Lp) to form, whereas goethite (Gt) was the predominant phase rather than Lp as pH increased to 7.2 with higher level Fe(II)aq. However, the La-substitution inhibits the La-Fh transformation rate and path. This made 5 % La-Fh transformed to Lp after 30 d at pH 7.2 and 1 mM Fe(II)aq. The La-release was present to some extent during the transformation process. Either the inhibition to La-Fh transformation or the La-slow release favors the retention of early-adsorbed Cr(VI) on iron-mineral surface. The added Cr(VI) might promote the La-Fh transformation even under a lower-level Fe(II), primarily attributed to the rapid reduction of Cr(VI) to Cr(III) by Fe(II), which produced more liable Fe(III) in addition to the Cr(III) that can improve the mineral conductivity, thereby enhancing the La-Fh transformation. Mechanisms responsible for the Fe(II)-induced La-Fh transformation companying the La slow-release are proposed to provide insights into the reduction and immobilization of Cr(VI) under anaerobic conditions, thus demonstrating that La-Fh is a promising material for application in Cr-contaminated site remediation.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
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IF 9
Xin Liu,Yong-De Yan,Yun Xue,Yue-Lin Wang,Qing-Guo Zhang,Guan-Qing Hu,Fu-Qiu Ma,Jing-Ping Wang
Waste resins produced from nuclear facilities operation always contain organic sulfur and radioisotope 65 Zn, which pose a significant threat for the ecological environment. This study proposed comparative research for treating cation exchange resins doped with Zn 2+ (Zn-CERs) in Na 2 CO 3 -K 2 CO 3 and Na 2 CO 3 -K 2 CO 3 -KOH. The enhanced removal effect of sulfur and Zn 2+ by KOH addition was explored. The energy dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) were carried out to investigate the element distribution and chemical states of C, O, S and Zn on Zn-CERs. The results of thermogravimetric analysis (TG) showed that the oxidation process of Zn-CERs was basically divided into three steps, and Zn 2+ acted as a catalyst for S-C destruction. The relatively mild reaction of Zn-CERs in Na 2 CO 3 -K 2 CO 3 -KOH largely avoids the escape of harmful gases (H 2 S and SO 2 ) caused by the overreaction. The participation of KOH could promote the sulfation of sulfides and sulfites, and the transformational SO 4 2− content reaches a maximum value (26.51 %) at 800 °C. When the temperatures above 700 °C, Zn 2+ and K + combines with SO 4 2− to form K 2 Zn 2 (SO 4 ) 3 . The proposed enhanced molten salt oxidation (MSO) system had excellent retention rates of Zn (99.56 %) and S (87.39 %) at 800 °C, which provides further technical references for the removal of organic sulfur and heavy metals.
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Molecular Catalysis
·
2024
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IF 3.9
Junsong Yue,Xiaochen Liu,Zhansheng Wu,Zhiyan Li,Jianwen Wang,Min Tu,Huaiqi Shi
To solve the problem that free glycosyltransferase is difficult to isolate and reuse in ginsenoside Rh2 catalysis and synthesis. In this study, we developed a magnetic nickel-based metal-organic framework (MOF) material (Fe 3 O 4/ PDA@MOF) to enable the separation of recombinant glycosyl transferase (UGT) directly from crude enzyme solutions, while simultaneously immobilizing it by utilizing the specific adsorption of Ni 2+ ions to His-tag. Our investigations demonstrated that the Fe 3 O 4 /PDA@MOF@UGT was successfully prepared, which not only maintained enzyme activity, but was reused by low-cost magnetic separation and exhibited enhanced enzyme loading and stability. Following thorough research and optimization, the immobilized enzyme achieved a rare ginsenoside Rh2 concentration of 69.68 µg/mL under optimal conditions, with an initial activity of 61.99 % after six cycles. This cost-effective, efficient, and sustainable biocomposite holds significant promise for industrial applications in enzyme purification and catalytic synthesis of ginsenoside.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shu Zhu,Chonghui Wei,Yuxiang Xia,Kewei Yu,Meiyun Chen,Yujie Zhao,Xingyu Luo
Uric acid (UA) is an important biomarker for assessing human health. The abnormal contents of UA in biological fluids are closely involved with many diseases. Although conventional methods for UA analysis with satisfied detection performances have been well-designed, they are still restricted by bulky instrument requirements and insufficiency during the detection processes. Herein, we have built a cellulose paper-based nanozyme detection platform (CF-cPADs) by immobilizing the cobalt-iron layered double hydroxide (CoFe LDH) for point-of-care (POC) detection of UA. In this assay, CoFe LDH with peroxidase (POD)-like activity has been synthesized to be immobilized onto the cellulose paper, and then the uricase (Uox) and CoFe LDH involved cascaded enzymatic reaction has been designed to realize the conversion from UA contents to colorimetric signals, which can be further analyzed by smartphone. The highly sensitive, stable, and reproducible CF-cPADs exhibit a wide linear range varied from 1 to 100 μM with a comparable limit of detection (LOD) as low as 0.92 μM for satisfying UA analysis in real samples. This approach can provide new insights into the development of cost-effective, portable, rapid, easy-to-use, and on-site detection of biomarkers. It will overcome the drawbacks in clinical diagnosis which are highly dependent on the professionals and analytical instruments.
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Scientific Reports
·
2015
·Vol. 5
·期 1
·1-13
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IF 5.578
Ye Yi Quan,Jin Chong Wei,Fan Shi Kai,Mao Qian Qian,Sun Cheng Liang,Yu Yan,Lin Xian Yong
Cell wall is the major component of root apoplast which is the main reservoir for iron in roots, while nitric oxide (NO) is involved in regulating the synthesis of cell wall. However, whether such regulation could influence the reutilization of iron stored in root apoplast remains unclear. In this study, we observed that iron deficiency elevated NO level in tomato ( Solanum lycopersicum ) roots. However, application of S-nitrosoglutathione, a NO donor, significantly enhanced iron retention in root apoplast of iron-deficient plants, accompanied with a decrease of iron level in xylem sap. Consequently, S-nitrosoglutathione treatment increased iron concentration in roots, but decreased it in shoots. The opposite was true for the NO scavenging treatment with 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). Interestingly, S-nitrosoglutathione treatment increased pectin methylesterase activity and decreased degree of pectin methylation in root cell wall of both iron-deficient and iron-sufficient plants, which led to an increased iron retention in pectin fraction, thus increasing the binding capacity of iron to the extracted cell wall. Altogether, these results suggested that iron-deficiency-induced elevation of NO increases iron immobilization in root apoplast by decreasing pectin methylation in cell wall.
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高影响因子
Frontiers in Chemistry
·
2019
·
IF 3.782
Xiao Yifan,Deng Sihui,Li Meng,Zhou Qixing,Xu Libang,Zhang Huaifang,Sun Dongmei,Tang Yawen
A feasible and green sol-gel method is proposed to fabricate well-distributed nano-particulate Fe-Ni2P incorporated in N, P-codoped porous carbon nanosheets (Fe-Ni2P@N,P-CNSs) using biomass agarose as a carbon source, and ethylenediamine tetra (methylenephosphonic acid) (EDTMPA) as both the N and P source. The doped Fe in Ni2P is essential for a substantial increase in intrinsic catalytic activity, while the combined N,P-containing porous carbon matrix with a better degree of graphitization endows the prepared Fe-Ni2P@N,P-CNSs catalyst with a high specific surface area and improved electrical conductivity. Benefiting from the specific chemical composition and designed active site structure, the as-synthesized Fe-Ni2P@N,P-CNSs manifests a satisfying catalytic performance toward both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) in an alkaline solution, with low overpotential, small Tafel slope and long-term durability, relative to the counterparts (Fe-free Ni12P5/Ni2P2O7@N,P-CNSs and CNSs) with single components and even comparable to Pt/C and RuO2 catalysts. The present work broadens the exploration of efficient bifunctional oxygen electrocatalysts using earth abundant biomass as carbon sources based on non-noble metals for low cost renewable energy conversion/storage.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Junya Liao,Shanshan Han,Xianglai Li,Jie He,Francesco Secundo,Hao Liang
Cascade reactions catalyzed by two or more enzymes have been widely used in industrial production and exhibited many advantages over the single-enzyme catalytic system. In this study, two components of hydroxylase monooxygenase (HpaBC) were first co-immobilized by Ni 2+ -nitrilotriacetic acid (Ni-NTA) functionalized magnetic silica nanoparticles (Ni-NTA/H 2 N-SiO 2 @Fe 3 O 4 ) for enhancing the stability and activity of biocatalysts with multi-components. These two components, HpaB and HpaC, were modified with histidine-tag and employed to construct a bi-enzyme catalytic system. After co-immobilization, the activity of the bi-enzyme system was 2.6 times of free enzymes. Meanwhile, the co-immobilized system was more stable against high temperature and alkaline condition, and maintained 76.6% of the initial activity for storage 12 days. Moreover, the co-immobilized HpaBC remained more than 60% catalytic activity after 7 cycles. These results showed that co-immobilized multi-component enzymes based on functionalized magnetic nanoparticles without purification would play a great potential role in the field of industrial biocatalysis.
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Polymers
·
2021
·Vol. 13
·期 2
·182
·
IF 4.329
Shuo Zeng,Jinwei Shi,Anchao Feng,Zhao Wang
This study aimed to modify an electrospun regenerated cellulose (RC) nanofiber membrane by surface grafting 2-(dimethylamino) ethyl methacrylate (DMAEMA) as a monomer via atom transfer radical polymerization (ATRP), as well as investigate the effects of ATRP conditions (i.e., initiation and polymerization) on enzyme immobilization. Various characterizations including XPS, FTIR spectra, and SEM images of nanofiber membranes before and after monomer grafting verified that poly (DMAEMA) chains/brushes were successfully grafted onto the RC nanofiber membrane. The effect of different ATRP conditions on laccase immobilization was investigated, and the results indicated that the optimal initiation and monomer grafting times were 1 and 2 h, respectively. The highest immobilization amount was obtained from the RC-Br-1h-poly (DMAEMA)-2h membrane (95.04 ± 4.35 mg), which increased by approximately 3.3 times compared to the initial RC membrane (28.57 ± 3.95 mg). All the results suggested that the optimization of initiation and polymerization conditions is a key factor that affects the enzyme immobilization amount, and the surface modification of the RC membrane by ATRP is a promising approach to develop an advanced enzyme carrier with a high enzyme loading capacity.Keywords:electrospinning;atom transfer radical polymerization;nanofiber membrane;enzyme immobilization
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