BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Liying Zhao,Huaixia Yang,Xiaoke Zheng,Jinge Li,Lihe Jian,Weisheng Feng,Jinming Kong
Cytokeratin fragment antigen 21–1 (CYFRA 21–1) DNA is a crucial biomarker closely associated with non-small cell lung cancer. Here, we fabricated a novel electrochemical biosensor for ultrasensitive detection of CYFRA 21–1 DNA via polysaccharide and electrochemically mediated atom transfer radical polymerization (eATRP) dual signal amplification . Specifically, thiolated peptide nucleic acid (PNA) probes at 5′-terminals are immobilized on the gold electrode surface for specific recognition of CYFRA 21–1 DNA (tDNA). After hybridization, hyaluronic acid (HA) is linked to the hybridized PNA/DNA duplexes via the recognized carboxylate-Zr 4+ -phosphate chemistry . Then multiple initiators of the polymerization reaction are introduced via esterification reaction. Lastly, large numbers of electro-active monomers are successfully grafted from the initiation sites of functionalized HA by eATRP reaction, significantly amplifying the electrochemical signal. Under optimal conditions, the constructed sensor can detect as low as 9.04 aM tDNA. Further, this proposed biosensor can also be applied to the direct detection of double-stranded DNA (dsDNA), obtaining 0.12 fM as the detection limit. Besides, this strategy shows high selectivity for mismatched bases and excellent applicability for CYFRA 21–1 DNA detection in the serum samples. Given its high sensitivity, selectivity, ease of operation, low cost and environmental friendliness , this biosensor has considerable potential in early diagnosis and biomedical application .
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Materials
·
2025
·Vol. 18
·期 5
·1131
·
IF 3.2
Tian Zhao
HKUST-1 (HKUST = Hong Kong University of Science and Technology) is one of the most recognized metal-organic frameworks (MOFs) based on copper and trimesate, extensively studied for a variety of applications, such as gas storage, separation, adsorption, electrocatalysis, drug delivery, sensor and photodegradation, etc. In this work, we introduce a novel nanofused HKUST-1, referred to as N-CuBTC (BTC = trimesate), which has been synthesized with the hydrothermal method at room temperature (typical synthesis temperature is from 80~120 °C). The resulting N-CuBTC features an irregular particle morphology, with numerous crystals clustering together and edges that have fused, creating a hierarchical pore structure. In contrast to the traditional micro-sized octahedral HKUST-1 (named as M-CuBTC), N-CuBTC displays a unique clumped morphology, where the HKUST-1 crystals are seamlessly integrated into a cohesive structure. This innovative formation significantly enhances mass transfer capabilities and porosity accessibility. Consequently, N-CuBTC demonstrates markedly improved catalytic performance in the cyanosilylation of aldehydes.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ziqian Wang,Chunyu Du,Ruyu Yan,Shuying Li,Guanyu Zheng,Dahu Ding
Polyhydroxyalkanoates (PHA) are promising candidates for replacing petroleum-derived plastics; however, their high production costs limit their commercialisation. In this study, we successfully isolated an efficient PHA-producing strain from a salt lake, which was subsequently identified as Halomonas sp. WZQ-1. Notably, Halomonas sp. WZQ-1 could serve as a promising cell-factory platform for polyhydroxybutyrate (PHB) production, achieving a comparatively high PHB productivity (7.64 ± 0.4 g L −1 ) under moderate salt stress (60 g L −1 NaCl). We further realised semi-continuous PHB production in a bench-scale fermenter at a steady state by irregularly replenishing the organic substrate. The maximum PHB concentration reached 12.13 g L −1 . Finally, we realised the non-sterile conversion of typical biowastes (e.g. pomelo and cantaloupe residues) to PHB using Halomonas sp. WZQ-1. Encouragingly, 4.36 g L −1 PHB was directly obtained from the hydrolysate of pomelo residues with a characteristic melting temperature of 174.0 °C. Life cycle assessment was employed to systematically evaluate the environmental sustainability and potential challenges of biowaste-driven PHB biorefineries. Overall, our findings could serve as a pivotal step toward the commercialisation of PHB and provide a valuable reference for PHB biorefineries.
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Gels
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2025
·Vol. 11
·期 8
·568
·
IF 5.3
Yi Wang,Enkai Lin,Ze Wang,Tong Feng,An Xie
Electrochromic (EC) devices are gaining increasing attention for next-generation smart windows and low-power displays due to their reversible color modulation, low operating voltage, and flexible form factors. Recently, electrochromic energy storage devices (EESDs) have emerged as a promising class of multifunctional systems, enabling simultaneous energy storage and real-time visual monitoring. In this study, we report a flexible dual-functional EESD constructed using polyaniline (PANI) films doped with anthraquinone-1-sulfonic acid sodium salt (AQS), coupled with a redox-active PVA-based gel electrolyte also incorporating AQS. The incorporation of AQS into both the polymer matrix and the gel electrolyte introduces synergistic redox activity, facilitating bidirectional Faradaic reactions at the film–electrolyte interface and within the bulk gel phase. The resulting vertically aligned PANI-AQS nanoneedle films provide high surface area and efficient ion pathways, while the AQS-doped gel electrolyte contributes to enhanced ionic conductivity and electrochemical stability. The device exhibits rapid and reversible color switching from light green to deep black (within 2 s), along with a high areal capacitance of 194.2 mF·cm−2at 1 mA·cm−2and 72.1% capacitance retention over 5000 cycles—representing a 31.5% improvement over undoped systems. These results highlight the critical role of redox-functionalized gel electrolytes in enhancing both the energy storage and optical performance of EESDs, offering a scalable strategy for multifunctional, gel-based electrochemical systems in wearable and smart electronics.
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Nature Communications
·
2022
·Vol. 13
·期 1
·1-16
·
IF 17.694
Yuan Xiaoxiao,Wang Ruirui,Han Bing,Sun ChengJun,Chen Ruimin,Wei Haiyan,Chen Linqi,Du Hongwei,Li Guimei,Yang Yu,Chen Xiaojuan,Cui Lanwei,Xu Zhenran,Fu Junfen,Wu Jin,Gu Wei,Chen Zhihong,Fang Xin,Yang Hongxiu,Su Zhe,Wu Jing,Li Qiuyue,Zhang Miaoying,Zhou Yufeng,Zhang Lei,Ji Guang,Luo Feihong
Gut dysbiosis has been linked to type 1 diabetes (T1D); however, microbial capacity in T1D remains unclear. Here, we integratively profiled gut microbial functional and metabolic alterations in children with new-onset T1D in independent cohorts and investigated the underlying mechanisms. In T1D, the microbiota was characterized by decreased butyrate production and bile acid metabolism and increased lipopolysaccharide biosynthesis at the species, gene, and metabolite levels. The combination of 18 bacterial species and fecal metabolites provided excellently discriminatory power for T1D. Gut microbiota from children with T1D induced elevated fasting glucose levels and declined insulin sensitivity in antibiotic-treated mice. In streptozotocin-induced T1D mice, butyrate and lipopolysaccharide exerted protective and destructive effects on islet structure and function, respectively. Lipopolysaccharide aggravated the pancreatic inflammatory response, while butyrate activated Insulin1 and Insulin2 gene expression. Our study revealed perturbed microbial functional and metabolic traits in T1D, providing potential avenues for microbiome-based prevention and intervention for T1D.
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Nano Today
·
2025
·
IF 10.9
Yating Qin,Yan Lin,Chao Tian,Yujie Qi,Shuling Wang,Xiaoyuan Chen,Wenxing Gu
Immune checkpoint blockade (ICB) therapy has emerged as a promising avenue for the treatment of malignant tumors. Nonetheless, the efficacy of this approach is constrained by low anti-tumor immunity and restricted intratumoral delivery of immune checkpoint inhibitors (ICIs) in solid tumors. In this study, a pH-responsive nanocomplex (BRM/PTX/aPD-1) was developed for the active transport of aPD-1 and PTX, thereby enhancing cancer chemoimmunotherapy. BRM/PTX/aPD-1 was prepared by using dynamic and reversible benzoic-imine bonds cross-linked bovine albumin (BSA), anti-PD-1 antibody (aPD-1) and PTX-loaded polylysine dendrimers. In the acidic tumor microenvironment, BRM/PTX/aPD-1 exhibited a charge reversal from negative to positive due to the hydrolysis of the β-carboxylic amide structure, which triggered cation-induced transcytosis. This process enabled active transport of adequate amounts of BRM/PTX/aPD-1 to the tumor parenchyma, where it was degraded by further hydrolysis of benzoic-imine bonds and effectively released aPD-1 and PTX. The nanocomplex demonstrated notable intratumoral permeability both in vitro and in vivo , particularly enhancing the delivery of aPD-1. Additionally, the nanocomplex showed robust anti-tumor activity in an orthotopic breast cancer model, significantly inhibiting tumor proliferation and preventing the progression of lung metastasis. This pH-responsive nanocomplex presents a promising platform for improved intratumoral delivery of ICIs and emerges as a practical solution for overcoming tumor immunosuppression.
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Journal of Zhejiang University-SCIENCE B
·
2021
·
IF 3.066
Meilin CUI,Yuchang MA,Youwei YU
Ganoderic triterpenoids (GTs) are the primary bioactive constituents of the Basidiomycotina fungus, Ganoderma lucidum . These compounds exhibit antitumor, anti-hyperlipidemic, and immune-modulatory pharmacological activities. This study focused on GT accumulation in mycelia of G. lucidum mediated by the heme oxygenase-1 (HO-1)/carbon monoxide (CO) signaling. Compared with the control, hemin (10 μmol/L) induced an increase of 60.1% in GT content and 57.1% in HO-1 activity. Moreover, carbon monoxide-releasing molecule-2 (CORM-2), CO donor, increased GT content by 56.0% and HO-1 activity by 18.1%. Zn protoporphyrin IX (ZnPPIX), a specific HO-1 inhibitor, significantly reduced GT content by 26.0% and HO-1 activity by 15.8%, while hemin supplementation reversed these effects. Transcriptome sequencing showed that HO-1/CO could function directly as a regulator involved in promoting GT accumulation by regulating gene expression in the mevalonate pathway, and modulating the reactive oxygen species (ROS) and Ca 2+ pathways. The results of this study may help enhance large-scale GT production and support further exploration of GT metabolic networks and relevant signaling cross-talk.
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Biomaterials Advances
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2022
·
IF 0
Ruimin Tang,Chunsheng Shao,Liangjian Chen,Li Yi,Bo Zhang,Jiangjie Tang,Weina Ma
Osseointegration between implants and bone tissue lays the foundation for the long-term stability of implants. The incorporation of a porous structure and local slow release of siRNA to silence casein kinase-2 interacting protein-1 (CKIP-1), a downregulator of bone formation, is expected to promote osseointegration. Here, porous implants with a porous outer layer and dense inner core were prepared by metal coinjection molding (MIM). Mg-doped calcium phosphate nanoparticles (CaPNPs)-grafted arginine-glycine-aspartate cell adhesion sequence (RGD) and transcribed activator (TAT) (MCPRT)/CKIP-1 siRNA complex and polylysine (PLL) were coated onto the surface of the porous implants by layer-by-layer (LBL) self-deposition. The in vitro results showed that the MCPRT-siRNA coating promoted MG63 cell adhesion and proliferation, enhanced the protein expressions (ALP and OC) and bone formation-related gene expression (OPN, OC and COL-1α) in vitro. The in vivo results demonstrated that the porous structure enhanced bone ingrowth and that the local slow release of MCPRT-siRNA accelerated new bone formation at the early stage. The porous structure coupled with local CKIP-1 siRNA delivery constitutes a promising approach to achieve faster and stronger osseointegration for dental implants.
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 23
·2200300
·
IF 6.575
Zhaodi Zhang,Jiahong Li,Ming Zhang,Binbin Li,XiaoHua Pan,Xiaoliang Dong,Li-Long Pan,Jia Sun
Scope Dietary fibers can alter gut microbiota and microbial metabolite profiles. SCFAs are produced by bacterial fermentation of fiber, mediating immune homeostasis through G-protein-coupled receptors (GPCRs). GPR109a, a receptor for niacin and butyrate, expressed by immune cells and non-immune cells, is a key factor regulating immune responses. However, the role and underlying mechanisms of GPR109a in type 1 diabetes (T1D) remain unclear. Methods and results Experimental T1D was induced by streptozotocin in GPR109a-deficient ( Gpr109a −/− ) and wild type mice. The study found that Gpr109a −/− mice were more susceptible to T1D with dysregulated immune responses, along with increased M1 macrophage polarization (from 10.55% to 21.48%). Further, an adoptive transfer experiment demonstrated that GPR109a-deficient macrophages promoted the homing of intestine-derived type 1 cytotoxic T cells to pancreas (from 18.91% to 24.24%), thus disturbing the pancreatic immune homeostasis in non-obese diabetic mice. Mechanistically, GPR109a deficiency promoted M1 macrophage polarization associated with the activation of suppressor of cytokine signaling 3-signal transducer and activator of transcription 1 signaling pathway. Conclusion The findings reveal that macrophage GPR109a deficiency accelerates the development of T1D. Activation of GPR109a on macrophage by dietary components may provide a new strategy for preventing or treating T1D.
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METABOLIC ENGINEERING
·
2017
·
IF 8.142
Lu Dai,Fei Tao,Hongzhi Tang,Yali Guo,Yaling Shen,Ping Xu
Primordial enzymes are proposed to possess broad specificities. Through divergence and evolution, enzymes have been refined to exhibit specificity towards one reaction or substrate, and are thus commonly assumed as “specialists”. However, some enzymes are “generalists” that catalyze a range of substrates and reactions. This property has been defined as enzyme promiscuity and is of great importance for the evolution of new functions. The promiscuities of two enzymes, namely glycerol dehydratase and diol dehydratase, were herein exploited for catalyzing long-chain polyols , including 1,2-butanediol, 1,2,4-butanetriol, erythritol , 1,2-pentanediol, 1,2,5-pentanetriol, and 1,2,6-hexanetriol. The specific activities required for catalyzing these six long-chain polyols were studied via in vitro enzyme assays , and the catalytic efficiencies were increased through protein engineering. The promiscuous functions were subsequently applied in vivo to establish 1,4-butanediol pathways from lignocellulose derived compounds, including xylose and erythritol. In addition, a pathway for 1-pentanol production from 1,2-pentanediol was also constructed. The results suggest that exploiting enzyme promiscuity is promising for exploring new catalysts, which would expand the repertoire of genetic elements available to synthetic biology and may provide a starting point for designing and engineering novel pathways for valuable chemicals.
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Journal of Superconductivity and Novel Magnetism
·
2025
·Vol. 38
·期 2
·112
·
IF 1.7
Li Xiaofeng,Gao Yuxia,Tian Zhaoming,Ren Weijie,Sun Xin,Wang Mingli
KNi(PO 3 ) 3 was successfully synthesized by solid-state method and the magnetic properties were characterized. The crystal structure of KNi(PO 3 ) 3 belongs to trigonal crystal system with space group R 3, where Ni 2+ ions form the distorted honeycomb-lattice within the ab-plane stacking in the “ABAB” type fashion along the c -axis. Magnetization measurements reveal the presence of antiferromagnetic (AFM) interaction with Curie Weiss (CW) temperature θ CW = − 12.905 K but without long-range magnetic order down to 2 K. Notably, the CW fitted effective moment µ eff = 3.35µ B and saturated magnetization M S = 2 μ B /Ni 2+ at 2 K support KNi(PO 3 ) 3 as an S = 1 spin system. Intriguingly, the zero-field specific heat exhibits a broad peak maximized at ~ 2.5 K indicating the onset of short-range spin correlation between Ni 2+ ions, while the integrated magnetic entropy ( S mag ) is close to the expected Rln3 for S = 1 system, indicative of large spin fluctuation below 2 K driven by spin frustration.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Leye Yan,Xinyan Hu,Jianan He,Hairun Gan,Yang Shi,Luting Li,Siyu Li,Kunpeng Wei,Jiaxing Wei,Jianxun Cai,Bing Li,Pengfei Pang
Atherosclerosis is a chronic inflammatory disease affecting arterial walls and remains a leading cause of morbidity and mortality worldwide. While anti-inflammatory therapy is widely recognized as a critical component in the management of atherosclerosis, current treatments are often limited by adverse effects and suboptimal efficacy. Membrane-derived nanovesicles have demonstrated excellent biocompatibility and prolonged circulation times, making them effective drug delivery carriers. However, their targeting capabilities remain limited, as only a small fraction of transmembrane proteins possesses intrinsic targeting functions. To overcome these limitations and leverage the targeting capability of folate in inflammatory disease, we designed biomimetic nanovesicles modified with folate by covalently conjugating folate-C 6 -PEG 4 -NHS to nanovesicles under physiological conditions enabling efficient drug delivery to atherosclerotic plaques. Meanwhile we also identified the PU.1 inhibitor DB1976 as an anti-inflammatory target for the treatment of atherosclerosis. Mechanistically, DB1976 suppresses inflammation by inhibiting the IL-1β/NF-κB signaling pathway, reducing reactive oxygen species (ROS) levels, and decreasing apoptosis rates. Folate-modified biomimetic nanovesicles loaded with DB1976 achieved promising therapeutic outcomes. In summary, folate-modified macrophage biomimetic nanovesicles loaded with a PU.1 inhibitor effectively alleviate atherosclerosis by targeting inflammation in atherosclerotic plaques, highlighting their potential as a promising therapeutic strategy for atherosclerosis treatment.
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