International Journal of Nanomedicine
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2019
·
IF 4.471
Tianfeng Yang,Guowen Du,Yuxin Cui,Runze Yu,Chen Hua,Wei Tian,Yanmin Zhang
Background Doxorubicin (DOX) is one of the most effective treatments for hepatocellular carcinoma (HCC), but is restricted by its poor pharmacokinetics. Herein, we exploited efficient targeted drug delivery systems and they have been found to be a worthy strategy for liver cancer therapy. Materials and methods We investigated polymeric nanoparticles which were synthesized based on host–guest interaction between β-cyclodextrin and benzimidazole. The properties of nanoparticles with regard to size/shape, encapsulation efficiency, and drug release were investigated using conventional experiments. Cell proliferation assay in vitro, cell uptake assay, and cell apoptosis analysis were used to investigate cytotoxicity, uptake, and mechanism of targeted supramolecular prodrug complexes (TSPCs)-based self-assemblies and supramolecular prodrug complexes (SPCs)-based self-assemblies. Results The pH-sensitive lactobionic acid (LA)-modified pH-sensitive self-assemblies were synthesized successfully. The results of in vitro released assay showed that the accelerated released of DOX from TSPCs-based self-assemblies with the decrease of pH value. When TSPCs-based self-assemblies were taken up by HepG2 cells, they demonstrated a faster release rate under acidic conditions and proved to have higher cytotoxicity than in the presence of LA. A mechanistic study revealed that TSPCs-based self-assemblies inhibited liver cell proliferation by inducing cell apoptosis. Conclusion The pH-sensitive nanocomplex, as liver-targeted nanoparticles, facilitated the efficacy of DOX in HepG2 cells, offering an appealing strategy for the treatment of HCC.
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Biomaterials Science
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2019
·Vol. 7
·期 5
·2144-2151
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IF 5.251
Qiang Zhang,Pengcheng Ma,Jiayang Xie,Si Zhang,Ximian Xiao,Zhongqian Qiao,Ning Shao,Min Zhou,Wenjing Zhang,Chengzhi Dai,Yuxin Qian,Fan Qi,Runhui Liu
Microbial infections have always been serious challenges to human health considering that antibiotics almost inevitably induce microbial resistance. Therefore, it is urgent to develop a new antibacterial agent that is active against drug-resistant bacteria and is less susceptible to microbial resistance. In this work, a series of host defense peptide (HDP) mimicking antibacterial poly-β-peptides were synthesized, characterized and evaluated for their biological activities. The best poly-β-peptide within this study (20 : 80 Bu : DM) displays potent and broad spectrum antibacterial activity against antibiotic-resistant super bugs and low toxicity toward mammalian cells. Moreover, these poly-β-peptides are bactericidal and kill bacteria very fast within 5 min. An antimicrobial resistance test demonstrated that bacteria develop no resistance toward the selected poly-β-peptides even over 1000 generations. Our studies demonstrate that random copolymers of heterochiral poly-β-peptides, without the need for defined secondary structures, can mimic the antimicrobial HDP. These results imply the potential application of these poly-β-peptides as new antimicrobial agents to tackle drug resistant antimicrobial infections.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2019
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IF 3.131
Xia Li,Xiao-Juan Nie,Yu-Nuo Zhu,Wen-Chao Ye,Yu-Lin Jiang,Shi-Long Su,Bi-Ting Yan
In this paper, a new β-cyclodextrin polymer (β-CDBEP) was successfully prepared by derivatizing with 1H-benzotriazole followed by cross-linking with epichlorohydrin . The adsorption behaviour of β-CDBEP for anionic Eriochrome Black T (EBT) was studied by batch experiments. The results showed that the highest adsorption capacity was obtained in acidic medium (pH = 5), and the pseudo-second-order model fitted well with the experimental data indicating the rate-determining step mainly determined by chemisorption . The adsorption equilibrium data followed better the Freundlich isotherm model with multi-layered adsorption compared to the Langmuir isotherm model. Thermodynamic parameters indicated that the adsorption of EBT onto β-CDBEP was an endothermic and spontaneous process, and a favorable adsorption happened at a relatively higher temperature. The combined electrostatic interactions , inclusion complexation, π-π interactions and intra-particle diffusion contributed to the adsorption of EBT onto β-CDBEP.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2019
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IF 5.107
Dongze Wang,Gang Chen,Xuemei Li,Qiong Jia
A β -cyclodextrin based hypercrosslinked polymer (Bn-CD-HCP) was prepared using 4,4′-bis(chloromethyl)-1,1′-biphenyl as the crosslinker via a Friedel-Crafts reaction. The Bn-CD-HCP material showed high surface area and thermal stability, making it an efficient adsorbent for albendazole, a benzimidazole carbamate. 8 was determined to be the best pH value for albendazole adsorption and the time necessary to reach equilibrium of adsorption was 30 min. The adsorption process agreed with the pseudo-second-order model and Langmuir isotherm model. A high adsorption capacity of 181.82 mg g −1 was achieved. The negative value of Gibbs free energy change suggested a spontaneous adsorption process. In addition, Bn-CD-HCP could be reused for albendazole adsorption for multiple cycles without significant losing its adsorption amount.
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Experimental and Therapeutic Medicine
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2019
·Vol. 18
·期 2
·1285-1290
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IF 1.448
Hanchun Chen,Qiong Lu,Chao Chen,Yunhai Di,Ya'nan Li,Weijie Min,Zhengquan Yu,Dongwei Dai
Mutations of the β‑catenin gene are common in various cancer types. MicroRNA (miR)‑24 suppresses gene expression during the cell cycle. However, the effects of miR‑24 on the cell viability and autophagy of glioma cells, and how these biological processes are regulated by β‑catenin are largely unclear. The current study aimed to investigate the role of β‑catenin in regulating the effects of miR‑24 on the cell viability and autophagy of glioma cells. The expression levels of microtubule‑associated proteins 1A/1B light chain 3B (LC3B) and Beclin1 were detected by immunohistochemistry and western blotting. Glioma C6 cells were transfected with miR‑24 mimics, miR‑24 inhibitors and negative control miRNAs. C6 cells transfected with miR‑24 mimics or negative control miRNAs were treated with the β‑catenin inhibitor, XAV‑939. An MTT assay was utilized to evaluate the viability of C6 cells. The expression of miR‑24 and mRNA expression of autophagy related 4a cysteine peptidase (ATG4A) were detected by quantitative polymerase chain reaction analysis. The protein expression of LC3B and Beclin1 decreased significantly in glioma tissue and glioma C6 cells compared with normal brain tissue. Compared with the negative control group, C6 cells transfected with miR‑24 mimics exhibited significantly higher cell viability at 24 and 48 h, and those transfected with miR‑24 inhibitors exhibited significantly lower cell viability at 48 h. XAV‑939 decreased the stimulatory effects of miR‑24 mimics on the viability of C6 cells. The expression of miR‑24 significantly decreased and ATG4A mRNA significantly increased in C6 cells transfected with XAV‑939 compared with those transfected with the negative control miRNA. XAV‑939 attenuated the miR‑24‑induced decrease of the protein expression of LC3B and Beclin1, and decreased the stimulatory effects of miR‑24 mimics on cell viability. In addition, XAV‑939 attenuated the miR‑24‑induced decrease of autophagy marker expression by attenuating miR‑24 expression and increasing ATG4A mRNA expression in glioma C6 cells. To the best of our knowledge, the present study is the first to demonstrate whether β‑catenin regulates the intracellular effects of miR‑24 on the viability and autophagy of glioma cells. The results also provide some mechanistic basis to the pharmaceutical targeting of WNT signaling in high grade glial tumors.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2019
·
IF 3.375
Kejing Wu,Junbo Wang,Yingming Zhu,Xueting Wang,Chunyan Yang,Yingying Liu,Changjun Liu,Houfang Lu,Bin Liang,Yongdan Li
The β-Mo2C on different carbon supports was prepared conveniently via direct H2 reduction or self-reduction for catalytic conversion of Kraft lignin into aromatic hydrocarbons, aromatic alcohols, and aromatic aldehydes. The β-Mo2C on macroporous carbon (MC) with large pore size exhibits the highest activity with aromatic yield of 0.543 g/g Kraft lignin at 280 °C for 3 h, resulting from the accessibility of lignin fragments to β-Mo2C. Mo, MoO2, and MoO3 possess relatively low activity. Higher calcination temperature is required to obtain β-Mo2C over graphite (Gr) support than MC and activated carbon (AC), and small grain size of Gr-supported catalysts is beneficial for high activity. The β-Mo2C on AC exhibits the highest selectivity to aromatic hydrocarbons, which are attributed to highly dispersed β-Mo2C in micropores.
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BIOELECTROCHEMISTRY
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2019
·
IF 4.474
Xiaohui Niu,Xing Yang,Zunli Mo,Ruibin Guo,Nijuan Liu,Pan Zhao,Zhenyu Liu
A facile approach was reported to synthesize β-cyclodextrin functionalized graphene that is bridged by 3,4,9,10-perylene tetracarboxylic acid (rGO-PTCA-CD) via a chemical route that involves the functionalization of rGO with PTCA followed by covalently cross-linking NH 2 -β-CD. The as-prepared rGO-PTCA-CD was characterized by Fourier transform infrared spectroscopy , scanning electron microscopy, X-ray photoelectron spectroscopy and electrochemical methods. The working electrodes were thoroughly studied for the cyclic voltammetry by using [Fe(CN) 6 ] 4−/3- as redox probe and using ferrocene as an internal standard. Furthermore, rGO-PTCA-CD was successfully applied to the recognition of phenylalanine enantiomers . The host-guest inclusion interaction between rGO-PTCA-CD and the phenylalanine enantiomers was investigated by differential pulse voltammetry with Fc used as a competitor. The recognition result showed that the rGO-PTCA-CD-modified glassy carbon electrode exhibited higher chiral recognition capability for L-Phe than for D-Phe with an enantioselectivity coefficient of 2.07. The proposed modified electrode had a limit of detection of 0.08 nM and 0.2 nM (S/N = 3) for L-Phe and D-Phe, respectively, with a linear response range of 0.01 mM to 5 mM, which was ascribed to the synergy of the rGO-PTCA (e.g., its excellent electrochemical performance) and β-CD (e.g., the hydrophobic inner cavity with good molecular recognition and enrichment abilities).
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CERAMICS INTERNATIONAL
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2019
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IF 3.45
Bo Wei,Jintang Zhou,Zhengjun Yao,Azhar Ali Haidry,Xinlu Guo,Haiyan Lin,Kun Qian,Wenjing Chen
In the present work, high purity cubic silicon carbide (β-SiC) was synthesized by using resorcinol-formaldehyde aerogel coated silica as precursor at high temperature. Subsequently, β-SiC was coated with various concentrations of silver nanoparticles (Ag NPs) by tin sensitization electroless plating . Thereafter, the effect of various Ag NPs contents on the dielectric and microwave absorption properties of β-SiC was investigated in detailed. It is found that the Ag NPs can significantly increase the overall complex permittivity, reduce the thickness of the absorber and increase the absorption bandwidth to some extent. Despite, the improvement of the attenuation ability of electromagnetic wave with increasing contents of Ag NPs also has an adverse effect on impedance matching. The improvement in the microwave absorption of β-SiC coated with Ag NPs mainly comes from the enhancement of dipole polarization, interface polarization and conductivity loss. In the 2–18 GHz band, Ag@SiC (1.0 g/L) can achieve an effective bandwidth of 4.99 GHz at a thickness of 1.6 mm, and it is a kind of lightweight, high-temperature microwave absorbent with excellent performance.
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ANALYTICA CHIMICA ACTA
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2019
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IF 5.256
Jimei Ma,Heng Zhang,Fangfang Peng,Xiaoqing Yang,Zi-Long Li,Linhao Sun,Hong Jiang
Carbon dots (CDs) were synthesized from p -aminophenol and ethylenediamine via one-step under mild condition and used as a sensitive fluorescent nanoprobe for the activity determination of N -acetyl-β- d -glucosaminidase (NAG). In this assay, p -nitrophenol was in situ produced from p -nitrophenyl-β-D- N -acetyl-glucosaminide, which was exclusively hydrolyzed by NAG. The UV absorption peak of p -nitrophenol (maximum at 400 nm) overlapped the excitation peak of CDs with maximum wavelength at 415 nm, which caused the fluorescence decline of CDs based on inner filter effect. The activity of NAG was determined by the fluorescence changes. The assay is highly sensitive to NAG with a low detection limit of 0.75 U L −1 (K = 3) and showed a good linear relationship in the range from 1 to 45 U L −1 . This CDs nanoprobe was successfully applied for the determination of NAG activity in human serum and urine samples.
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高影响因子
CARBOHYDRATE POLYMERS
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2019
·
IF 6.044
Yi Shi,Yan Zhang,Yanru Cui,Jianrong Shi,Xiaoyan Meng,Jingyi Zhang,Hua He
Here, a porous superparamagnetic composite material magnetic β-cyclodextrin polymer (MNPs/β-CDP) was prepared and characterized as a adsorbent that may be used for the degradaion of acetaminophen (APAP) in aqueous solution by coupling with potassium permanganate (KMnO 4 ) oxidation. The results of adsorption properties investigation showed that the adsorption rate and capacity of MNPs/β-CDP were better than the component materials. When coupled with KMnO 4 oxidation, the degradation efficiency were evaluated with regards to the pseudo-first-order kinetics of 4-CP degradation. As an oxidation reactor, the material effectively alleviated the secondary pollution and water coloration caused by oxidation of KMnO 4 . Moreover, the materials were directly regenerated by the reaction of KMnO 4 with APAP adsorbed in its cavities without additional elution treatment, making it more convenient and environmentally friendly. The repeated degradation experiments demonstrated that MNPs/β-CDP had mechanic stability and reusability. The findings provide novel ideas for the degradation of persistent contaminants in water environments.
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JOURNAL OF ALZHEIMERS DISEASE
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2018
·Vol. 64
·期 1
·309-322
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IF 3.476
Du Yehong,Fu Min,Wang Yu Tian,Dong Zhifang
Alzheimer’s disease (AD) is a neurodegenerative disease characterized by the deposition of amyloid-β peptides (Aβ). Aβ accumulation leads to the formation of neurofibrillary tangles, inflammation, axonal injury, synapse loss, and neuronal apoptosis. Thus, reducing Aβ levels should exert a neuroprotective effect against AD. Ginsenoside Rf, an extract from Panax notoginseng, has potent anti-fatigue, anti-nociception, anti-oxidation, and anti-inflammation properties. However, it is unclear whether ginsenoside Rf is effective in the treatment of AD. Here, we reported that ginsenoside Rf could significantly attenuate Aβ-induced apoptosis in N2A cells, as reflected by a dramatic increase in mitochondrial membrane potential and decrease in Ca2 + concentration, reactive oxygen species, and active caspase-3 expression. Meanwhile, ginsenoside Rf could alleviate the Aβ-induced inflammation reaction, such as the decrease of interferon-gamma (IFN-γ) and active caspase-1 expression and the increase of interleukin-13. Furthermore, we also found that Rf is able to accelerate Aβ clearance and subsequently reduces Aβ level in N2A cells stably transfected with human Swedish mutant APP695 (N2A-APP). More importantly, daily Rf treatment (20 mg/kg, i.p.) throughout the experiment dramatically improved spatial learning and memory in Aβ42-induced mouse model of AD. Taken together, these results indicate that ginsenoside Rf may decrease Aβ-induced neurotoxicity and memory decline via anti-inflammatory response during AD development, suggesting that Rf may be a potential therapeutic agent for treating AD.
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ACS Omega
·
2018
·
IF 0
Wen Qi,Qian Wu,Joseph G. Shapter,Meng Duan,Sheng Yang,Guo Gao
Chirality, universal characteristics of nature, introduces asymmetry in synthetic materials. Revealing the microscopic asymmetry of macroscopically symmetric materials is the key to control the growth of chiral materials and give full play to their application potential. Materials for photon upconversion (UC) are of great interest for many applications owing to their anti-Stoke luminescence process, especially for chiral UC materials. For the preparation of UC materials, a tiny change in reaction parameters will lead to variations in morphology, phase components, and fluorescence intensity, as well as its chirality. Because of the strict reaction conditions for the formation of chiral UC materials, there are no reports of the successful synthesis of chiral UC materials. Therefore, a facile method for the controllable synthesis of chiral UC materials is highly desired. Herein, chiral-assembled hexagonal prisms of β-NaYF4:Er3+/Yb3+ microcrystals were synthesized to realize the smart manipulation of their morphology as well as a great improvement of the fluorescence efficiency. We proposed a three-stage doped β-NaYF4 crystal growth mechanism on mesoscale regulation, where the fluorescence enhancement principle of chirality was revealed. The enhancement of fluorescence efficiency of chiral UC materials endows their promising application in luminescent displays.
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