Nature Communications
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2022
·Vol. 13
·期 1
·1-16
·
IF 17.694
Yuan Xiaoxiao,Wang Ruirui,Han Bing,Sun ChengJun,Chen Ruimin,Wei Haiyan,Chen Linqi,Du Hongwei,Li Guimei,Yang Yu,Chen Xiaojuan,Cui Lanwei,Xu Zhenran,Fu Junfen,Wu Jin,Gu Wei,Chen Zhihong,Fang Xin,Yang Hongxiu,Su Zhe,Wu Jing,Li Qiuyue,Zhang Miaoying,Zhou Yufeng,Zhang Lei,Ji Guang,Luo Feihong
Gut dysbiosis has been linked to type 1 diabetes (T1D); however, microbial capacity in T1D remains unclear. Here, we integratively profiled gut microbial functional and metabolic alterations in children with new-onset T1D in independent cohorts and investigated the underlying mechanisms. In T1D, the microbiota was characterized by decreased butyrate production and bile acid metabolism and increased lipopolysaccharide biosynthesis at the species, gene, and metabolite levels. The combination of 18 bacterial species and fecal metabolites provided excellently discriminatory power for T1D. Gut microbiota from children with T1D induced elevated fasting glucose levels and declined insulin sensitivity in antibiotic-treated mice. In streptozotocin-induced T1D mice, butyrate and lipopolysaccharide exerted protective and destructive effects on islet structure and function, respectively. Lipopolysaccharide aggravated the pancreatic inflammatory response, while butyrate activated Insulin1 and Insulin2 gene expression. Our study revealed perturbed microbial functional and metabolic traits in T1D, providing potential avenues for microbiome-based prevention and intervention for T1D.
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Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Yifei Chen,Yongru You,Chen Xin,Xiuqin Dong,Hao Gong
The diverse pore structures and excellent tunable proprieties of IRMOFs materials made the possibility to explore the effect of the mode of ligand-immobilization on its catalytic performance for 1-butene hydroformylation. In this work, we report a successful case of theory-guided rational design of a highly active ligand-immobilized Rh/IRMOF catalyst based on previous work. Density functional theory calculations of elementary reaction barriers for 1-butene hydroformylation over 1Rh/IRMOFs-PPh 3 models (IRMOF-1, -8, -10, -14, and -16) were performed. The calculation results and topographic steric maps analysis predicted that 1Rh/IRMOF-10-PPh 3 have superior catalytic performance. This can be attributed to the “shape-selective” effect of phosphine ligands grafted in the backbone on the reactive transition state. In addition, the anchoring positions of the phenyl phosphine ligand grafting on the skeleton of 1Rh/IRMOF-10-PPh 3 was identified in details. The 1Rh/IRMOF-10-PPh 3 [3‐3] was predicted to have the highest n/i ratio. In order to verify the theoretical prediction, 1Rh/IRMOF-10–32PPh 3 catalyst was prepared by post-synthesis strategy and the n/i ratio was experimentally confirmed to be 3.49, which outperform the previous 1Rh/MOF-5-PPh 3 catalyst. This work suggests that the 1Rh/IRMOF-10-PPh 3 catalyst can be a promising catalyst for hydroformylation reactions.
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Foods
·
2024
·Vol. 13
·期 23
·3797
·
IF 4.7
Chenmeng Song,Wei Zheng,Chengyi Song,Houfeng Zhou,Jengyuan Yao
Background: Kaempferol (KPF), a flavonoid abundant in edible plants, possesses potent anti-inflammatory and antioxidant properties beneficial with notable health benefits. Objective: To evaluate the protective effects of KPF on metabolic disturbances and pancreatic damage in a Type 1 diabetes mellitus (T1DM) mouse model. Methods: Male C57BL/6 mice were divided into normal, T1DM, T1DM + KPF 25 mg/kg, and T1DM + KPF 50 mg/kg groups. T1DM was induced by streptozotocin (STZ). KPF was administered via intraperitoneal injection for 2 weeks. After 4 weeks from the start, metabolic parameters, pancreatic histology, and plasma metabolites were analyzed. Network pharmacology and molecular docking identified key targets and pathways. In vitro, INS-1 cells were used to assess reactive oxygen species (ROS) production and apoptosis. Results: KPF significantly reduced blood glucose (GLU) and triglyceride (TG) levels, increased high-density lipoprotein (HDL) levels, and preserved pancreatic β-cell structure. Metabolomics revealed changes in energy metabolism and oxidative stress-related metabolites. Network analysis highlighted the PI3K/AKT/mTOR pathway, with strong binding affinities to targets such as AKT1. In vitro, KPF decreased ROS production in INS-1 cells; this effect was reversed by a PI3K/AKT inhibitor. KPF also reduced apoptosis in INS-1 cells. Conclusions: KPF ameliorates metabolic disturbances and pancreatic damage in T1DM mice, suggesting potential as a functional food ingredient for diabetes management.
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ADVANCED FUNCTIONAL MATERIALS
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2025
·
IF 19
Biao Zeng,Hao Yuan,Zhen Guo,Xinmeng Er,Yifei Liu,Bohua Dong,Fei Ma
Ultrafine nanocatalysts can provide abundant catalytic sites, but typically it is less stable and susceptible to dissolution diffusion. Here, this work reports a selenium-mediated strategy to construct sub-nanometers amorphous shell on ultrafine Pt nanocrystal core, forming a Pt@PtSe x core/shell nanostructure. In this developed strategy, the active Se-oleylamine precursor after Pt nucleation confines the overgrowth and oriented attachment of Pt, allowing the in situ formation of core/shell nanostructures combining ultrafine size and sub-nano amorphous shell. Essentially, the sub-nano amorphous PtSe x shell can not only optimize the hydrogen adsorption energy and lower the water dissociation energy, but also retard the dissolution of Pt atoms, thus leading to significantly improved activity and durability in both acidic and alkaline electrolytes. The overall water splitting can be driven by a small cell voltage of 1.48/1.53 V at 10 mA cm −2 in 0.5 M H 2 SO 4 /1.0 M KOH, respectively. Moreover, Pt@PtSe x -1 with ≈0.8 nm thick amorphous shell exhibits ultra-high mass activity of 75.2 A mg Pt −1 in acidic and 18.4 A mg Pt −1 in alkaline at −70 mV, as well as a robust durability with stable operation for 1100 h at 100 mA cm −2 in alkaline (1300 h at 160 mA cm −2 in acidic), superior to the most reported Pt-based electrocatalysts.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2014
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IF 2.129
Shuya Cui,Tao Wang,Xiaoli Hu
A new chiral ionic liquid was synthesized from (S)-1-phenylethylamine and it was studied by IR, Raman, polarimetry, NMR and X-ray crystal diffraction. Its vibrational spectral bands are precisely ascribed to the studied structure with the aid of DFT theoretical calculations. The optimized geometries and calculated vibrational frequencies are evaluated via comparison with experimental values. The vibrational spectral data obtained from IR and Raman spectra are assigned based on the results of the theoretical calculations by the DFT-B3LYP method at 6-311G(d,p) level. The computed vibrational frequencies were scaled by scale factors to yield a good agreement with observed experimental vibrational frequencies. The vibrational modes assignments were performed by using the animation option of GaussView5.0 graphical interface for Gaussian program.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2021
·
IF 4.539
Meng Yang,Hao Guo,Lei Sun,Ning Wu,Mingyue Wang,Fan Yang,Tingting Zhang,Junye Zhang,Zhilan Pan,Wu Yang
In this present work, a carboxylated multi-walled carbon nanotubes (MWCNT-COOH) and covalent triazine framework (CTF-1) co-modified glassy carbon electrode (GCE) is prepared and used for simultaneous electrochemical detection of hydroquinone (HQ) and catechol (CC). Due to the synergistic effect between the high electrocatalytic activity and good conductivity of MWCNT-COOH and large surface area of CTF-1, the prepared modified electrode exhibits good selectivity and high sensitivity for the simultaneous determination of HQ and CC. In the neutral phosphate buffer, HQ and CC produce a pair of well-defined redox peaks on the MWCNT-COOH/CTF-1/GCE. The concentrations of CC and HQ show good linearity in the range of 2–280 μM and the detection limits of HQ and CC are 0.12 μM and 0.17 μM, respectively. The prepared electrochemical sensor can be employed to determine contents of HQ and CC in real samples.
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VIROLOGICA SINICA
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2012
·Vol. 27
·期 1
·19-25
·
IF 0
Liang Yuan-Yuan,Wang Bin,Qian Dong-Meng,Li Ling,Wang Zhi-Hao,Hu Ming,Song Xu-Xia
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were used to detect the inhibitory effects of GRb1 on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay. We found that in the 400 μg/mL GRb1 and 400 μg/mL GRb1+HSV-1 groups, MTT values were higher than control group at all times ( P <0. 05). Moreover, the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group ( P <0. 05). These results confirmed that, at appropriate concentrations, GRb1 could inhibit nerve cell apoptosis in HSV-1 infections.
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METABOLIC ENGINEERING
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2017
·
IF 8.142
Lu Dai,Fei Tao,Hongzhi Tang,Yali Guo,Yaling Shen,Ping Xu
Primordial enzymes are proposed to possess broad specificities. Through divergence and evolution, enzymes have been refined to exhibit specificity towards one reaction or substrate, and are thus commonly assumed as “specialists”. However, some enzymes are “generalists” that catalyze a range of substrates and reactions. This property has been defined as enzyme promiscuity and is of great importance for the evolution of new functions. The promiscuities of two enzymes, namely glycerol dehydratase and diol dehydratase, were herein exploited for catalyzing long-chain polyols , including 1,2-butanediol, 1,2,4-butanetriol, erythritol , 1,2-pentanediol, 1,2,5-pentanetriol, and 1,2,6-hexanetriol. The specific activities required for catalyzing these six long-chain polyols were studied via in vitro enzyme assays , and the catalytic efficiencies were increased through protein engineering. The promiscuous functions were subsequently applied in vivo to establish 1,4-butanediol pathways from lignocellulose derived compounds, including xylose and erythritol. In addition, a pathway for 1-pentanol production from 1,2-pentanediol was also constructed. The results suggest that exploiting enzyme promiscuity is promising for exploring new catalysts, which would expand the repertoire of genetic elements available to synthetic biology and may provide a starting point for designing and engineering novel pathways for valuable chemicals.
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International Journal of Nanomedicine
·
2023
·
IF 8
Xiaofeng Chen,Yu Liu,Leiying Miao,Yangyang Wang,Shuangshuang Ren,Xuebin Yang,Yong Hu,Weibin Sun
Periodontitis is a major cause for tooth loss, which affects about 15% of the adult population. Cementum regeneration has been the crux of constructing the periodontal complex. Cementum protein 1 (CEMP1) is a cementum-specific protein that can induce cementogenic differentiation. In this study, poly(ethylene glycol) (PEG)-stabilized amorphous calcium phosphate (ACP) nanoparticles were prepared by wet-chemical method and then loaded with recombinant human CEMP1 (rhCEMP1) for controlled release. An electrospun multiphasic scaffold constituted of poly(ε-caprolactone) (PCL), type I collagen (COL), and rhCEMP1/ACP was fabricated. The effects of rhCEMP1/ACP/PCL/COL scaffold on the attachment proliferation, osteogenic, and cementogenic differentiations of human periodontal ligament cells, (PDLCs) were systematically investigated. A critical size defect rat model was introduced to evaluate the effect of tissue regeneration of the scaffolds in vivo. The results showed that PEG-stabilized ACP nanoparticles formed a core-shell structure with sustained release of rhCEMP1 for up to 4 weeks. rhCEMP1/ACP/PCL/COL scaffold could suppress PDLCs proliferation behavior and upregulate the expression of cementoblastic markers including CEMP1 and cementum attachment protein while downregulating osteoblastic markers including osteocalcin and osteopontin when it was cocultured with PDLCs in vitro for 7 days. Histology analysis of cementum after being implanted with the scaffold in rats for 8 weeks showed that there was cementum-like tissue formation but little bone formation. These results indicated the potential of using electrospun multiphasic scaffolds for controlled release of rhCEMP1 for promoting cementum regeneration in reconstruction of the periodontal complex.
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Frontiers in Genetics
·
2022
·
IF 4.772
Shan Mengjie,Liu Hao,Hao Yan,Song Kexin,Meng Tian,Feng Cheng,Wang Youbin,Huang Yongsheng
Background: Keloid is a skin fibroproliferative disease with unknown pathogenesis. Metabolomics provides a new perspective for revealing biomarkers related to metabolites and their metabolic mechanisms.Method: Metabolomics and transcriptomics were used for data analysis. Quality control of the data was performed to standardize the data. Principal component analysis (PCA), PLS-DA, OPLS-DA, univariate analysis, CIBERSORT, neural network model, and machine learning correlation analysis were used to calculate differential metabolites. The molecular mechanisms of characteristic metabolites and differentially expressed genes were identified through enrichment analysis and topological analysis.Result: Compared with normal tissue, lipids have a tendency to decrease in keloids, while peptides have a tendency to increase in keloids. Significantly different metabolites between the two groups were identified by random forest analysis, including 1-methylnicotinamide, 4-hydroxyproline, 5-hydroxylysine, and l-prolinamide. The metabolic pathways which play important roles in the pathogenesis of keloids included arachidonic acid metabolism and d-arginine and d-ornithine metabolism. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide are metabolic indicators of keloid severity. The high-risk early warning index for 5-hydroxylysine is 4 × 108-6.3×108 (p = 0.0008), and the high-risk predictive index for 1-methylnicotinamide is 0.95 × 107-1.6×107 (p = 0.0022).Conclusion: This study was the first to reveal the metabolome profile and transcriptome of keloids. Differential metabolites and metabolic pathways were calculated by machine learning. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide may be metabolic indicators of keloid severity.
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Frontiers of Medicine
·
2021
·Vol. 15
·期 6
·887-902
·
IF 4.592
Wu Xin,Wang Zongqi,Li Haiying,Xie Xueshun,Wu Jiang,Shen Haitao,Li Xiang,Wang Zhong,Chen Gang
Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2 + SOX10 + oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.
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Journal of Dental Sciences
·
2026
·Vol. 21
·期 4
·2375
·
IF 3.6
Jie Li,Changqing Yan,Xinyi Sun,Chenying Zhang,Xiaozhe Wang,Yang Liu,Shuguo Zheng
Background/purpose Cleidocranial dysplasia (CCD), caused by Runt-related transcription factor 2 (RUNX2) haploinsufficiency, features delayed tooth eruption. Although impaired osteoclastogenesis contributes to eruption failure, the direct molecular mechanisms linking RUNX2 deficiency to osteoclast dysfunction remain elusive. Sirtuin 1 (SIRT1) is a critical regulator of osteoclastogenesis; however, whether it serves as a downstream effector of RUNX2 remains unexplored. This study aimed to elucidate the mechanistic link between RUNX2 and SIRT1 in regulating osteoclast differentiation and tooth eruption. Materials and methods Tooth eruption and osteoclast differentiation were assessed by microcomputed tomography and histological analysis in Runx2 +/− mice. Chromatin immunoprecipitation quantitative polymerase chain reaction and luciferase reporter assays confirmed RUNX2 binding to the Sirt1 promoter. Osteoclast marker expression was evaluated by reverse transcription quantitative polymerase chain reaction and western blotting. Bone marrow-derived macrophages from Runx2 +/− mice were transfected with small interfering RNA targeting SIRT1 for rescue experiments. Osteoclast differentiation and function were assessed by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays. Results Runx2 +/− mice showed significantly reduced mandibular first molar eruption and decreased TRAP-positive osteoclasts, accompanied by SIRT1 upregulation. RUNX2 directly bound to the Sirt1 promoter and repressed its transcription. SIRT1 knockdown increased expression levels of osteoclast markers and elevated acetylation of NF-κB p65 at lysine 310. Importantly, SIRT1 knockdown rescued osteoclast formation and resorption to wild-type levels in Runx2 +/− cells. Conclusion RUNX2 haploinsufficiency impairs tooth eruption through transcriptional derepression of SIRT1, which inhibits osteoclastogenesis via NF-κB p65 deacetylation, highlighting the RUNX2-SIRT1 axis as a novel therapeutic target for CCD.
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