INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Bin Ma,Jiafeng Niu,Hao Zhu,Huibing Chi,Zhaoxin Lu,Fengxia Lu,Ping Zhu
The oxidative reaction of Fusarium mycotoxin deoxynivalenol (DON) using the dehydrogenase is a desirable strategy and environmentally friendly to mitigate its toxicity. However, a critical issue for these dehydrogenases shows widespread substrate promiscuity. In this study, we conducted pocket reshaping of Devosia strain A6-243 pyrroloquinoline quinone (PQQ)-dependent dehydrogenase (DADH) on the basis of protein structure and kinetic analysis of substrate libraries to improve preference for particular substrate DON (10a). The variant presented an increased preference for substrate 10a and enhanced catalytic efficiency. A 4.7-fold increase in preference for substrate 10a was observed. Kinetic profiling and molecular dynamics (MD) simulations provided insights into the enhanced substrate specificity and activity. Moreover, the variant exhibited stronger conversion of substrate 10a to 3-keto-DON compared to the wild DADH. Overall, this study provides a feasible protocol for the redesign of PQQ-dependent dehydrogenases with favourable substrate specificity and catalytic activity, which is desperately needed for DON antidote development.
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高影响因子
CARBON
·
2025
·
IF 11.6
Jin Chen,Pei Liu,Kai Xu,Zhilei Hao,Guohui Tang,Changtian Zhu,Zixuan Ding,Shuai Yin,Zhiqiang Li,Zhen Ding,Yi Wang,Zhanpeng Liu,Siwen Yu,Xing Xin,Fenghua Liu
Na 3 V 2 (PO 4 ) 3 (NVP) material has evolved as a significant candidate for electrode materials in the development of sodium-ion batteries. However, the low conductivity of NVP material leads to low cycling performance, limiting their application in high-efficiency battery materials. To address this issue, this work proposes a novel strategy for materials design through constructing 3D network carbon-coatings on the surface of NVP materials. The carbon-coatings are achieved via a combination of sol-gel technique and heat treatment using pyrolytic carbon (C), carbon nanotubes (CNTs) and graphene (GN). In comparison to NVP@C, the electrochemical properties of NVP@C/GN, NVP@C/CNTs, and NVP@C/GN/CNTs have shown improvements. Among these, NVP@C/GN/CNTs exhibit the most outstanding electrochemical performance in the half-cell test. Specifically, this material demonstrates exceptional cycling stability, with a capacity of 92 mAh g −1 that can be maintained even after 800 cycles at a high-rate performance of 10C, with an attenuation of 10.6 %. The enhanced electrochemical performance is attributed to the specific ternary carbon-coating conductive structure, which significantly shortens the diffusion paths of Na + ions and electrons within NVP, resulting in improved Na + ion and electron transport kinetics. This study provides a new design paradigm for improving the conductivity of cathode material for sodium-ion batteries.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 7
·1-8
·
IF 5.3
Han Lei,Cai Shuanglong,Chen Xiaogeng
The development of an ultrasensitive and precise measurement of a breast cancer biomarker (cancer antigen 15-3; CA15-3) in complex human serum is essential for the early diagnosis of cancer in groups of healthy populations and the treatment of patients. However, currently available testing technologies suffer from insufficient sensitivity toward CA15-3, which severely limits early large-scale screening of breast cancer patients. We report a versatile electrochemical immunoassay method based on atomically cobalt-dispersed nitrogen-doped carbon (Co-NC)–modified disposable screen-printed carbon electrode (SPCE) with alkaline phosphatase (ALP) and its metabolite, ascorbic acid 2-phosphate (AAP), as the electrochemical labeling and redox signaling unit for sensitive detection of low-abundance CA15-3. During electrochemical detection by differential pulse voltammetry (DPV), it was found that the Co-NC-SPCE electrode did not have a current signal response to the AAP substrate; however, it had an extremely favorable response current to ascorbic acid (AA). Based on the above principle, the target CA15-3-triggered immunoassay enriched ALP-catalyzed AAP produces a large amount of AA, resulting in a significant change in the system current signal, thereby realizing the highly sensitive detection of CA15-3. Under the optimal AAP substrate concentration and ALP catalysis time, the Co-NC-SPCE-based electrochemical immunoassay demonstrated a good DPV current for CA15-3 in the assay interval of 1.0 mU/mL to 10,000 mU/mL, with a calculated limit of detection of 0.38 mU/mL. Since Co-NC-SPCE has an excellent DPV current response to AA and employs split-type scheme, the constructed electrochemical immunoassay has the merits of high preciseness and anti-interference, and its clinical diagnostic results are comparable to those of commercial kits. Graphical abstract
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高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Jinkui Wang,Tong Bu,Qianqian Cui,Zizhe Li,Qing Xu,Zhixian Jiang,Yexuan Mao,Xianqing Huang,Lianjun Song,Dapeng Peng,Xiya Zhang
Simultaneous detection of carbofuran (CBF) and 3-hydroxy carbofuran (3-OH-CBF) in fruits and vegetables is important due to their high toxicity and widespread use in pest control. However, most lateral flow immunoassay (LFA) approaches only detection CBF. To overcome this limitation, two haptens, 6-((2,2-dimethyl-2,3-dihydrobenzofuran-7-yl)oxy)hexanoic acid and 6-((((2,2-dimethyl-2,3-dihydroben zofuran-7-yl)oxy)carbonyl)amino)hexanoic acid, named H1 and H2, were designed for broad-spectrum antibody detection of CBF and 3-OH-CBF. The highly specific monoclonal antibody (mAb) 3F4 based on hapten H1 only recognized CBF, while broad-spectrum mAbs 1D3, 6B7 and 6E8 based on hapten H2 recognized both compounds. The carbon bond to the hydroxyl group of 3-OH-CBF adopts a 28.55° angle relative to the same carbon bond of CBF, explaining the specificity of the H1-based mAb against CBF. After optimizing the antigen-antibody combination, a sensitive colloidal gold-LFA (CG-LFA) strip was constructed based on mAb 1D3 and H2-BSA for CBF and 3-OH-CBF detection following simple sample pretreatment. The established LFA had a limit of detection of 0.49–6.63 ng/mL, and adequate recoveries (69.3–105.7 %) for CBF and 3-OH-CBF in 26 fruit and vegetable samples.
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高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Xiaoxu Wang,Longyi Nan,Haoyuan Song,Jiaqi Wang,Shuai Zhang,Yu Cai,Jie Liu,Jianpeng Guo,Liang Fang
In transdermal patches, pressure-sensitive adhesives (PSAs) are considered the most critical excipients due to their ability to provide adhesion and act as a drug reservoir. However, the loaded drugs plasticize the patches, resulting in a cold flow phenomenon, which poses a product safety risk and reduces treatment efficacy. Herein, 3-methacryloxypropyltrimethoxysilane (KH-570)-modified hydroxyl acrylate PSA (AAOK) was proposed. Diclofenac, ketoprofen, tulobuterol, and oxybutynin were selected as model drugs. The results showed that when not loaded with drugs, the performance of AAOK was not significantly different from that of hydroxyl acrylate PSA (AAOH). Interestingly, when loaded with drugs, AAOK automatically forms a cross-linked system through drug catalysis, which exerts a strong antiplasticity without reducing the ability of AAOK to load and deliver the drug. Specifically, the shear strength of drug-loaded AAOK increased by more than 10-fold compared to AAOH. The cold flow area of drug-loaded AAOK was reduced by more than 15-fold compared to commercial DURO-TAK 87-2287. In addition, the “dark ring” phenomenon was not observed with AAOK compared to that with DURO-TAK 87-2287 on the human forearm after drug loading. After loading four model drugs into AAOK, a high drug loading and efficient drug delivery were endowed for AAOK in vitro and in vivo. The molecular mechanism for the improved antiplasticity of the drug-loaded AAOK was that the drug molecules had a catalyzing effect on the self-condensation reactions of KH-570. This study opens up ideas for the investigation of transdermal patches with high antiplasticity and efficient transdermal application.
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Neural Regeneration Research
·
2024
·Vol. 19
·期 10
·2270
·
IF 5.9
Qi Yize,Zhou Yao,Li Jiyang,Zhu Fangyuan,Guo Gengni,Wang Can,Yu Man,Wang Yijie,Ma Tengfei,Feng Shanwu,Zhou Li
Methamphetamine addiction is a brain disorder characterized by persistent drug-seeking behavior, which has been linked with aberrant synaptic plasticity. An increasing body of evidence suggests that aberrant synaptic plasticity is associated with the activation of the NOD-like receptor family pyrin domain containing-3 (NLRP3) inflammasome. 3′-Deoxyadenosin, an active component of the Chinese fungus Cordyceps militaris, has strong anti-inflammatory effects. However, whether 3′-deoxyadenosin attenuates methamphetamine-induced aberrant synaptic plasticity via an NLRP3-mediated inflammatory mechanism remains unclear. We first observed that 3′-deoxyadenosin attenuated conditioned place preference scores in methamphetamine-treated mice and decreased the expression of c-fos in hippocampal neurons. Furthermore, we found that 3′-deoxyadenosin reduced the aberrant potentiation of glutamatergic transmission and restored the methamphetamine-induced impairment of synaptic plasticity. We also found that 3′-deoxyadenosin decreased the expression of NLRP3 and neuronal injury. Importantly, a direct NLRP3 deficiency reduced methamphetamine-induced seeking behavior, attenuated the impaired synaptic plasticity, and prevented neuronal damage. Finally, NLRP3 activation reversed the effect of 3′-deoxyadenosin on behavior and synaptic plasticity, suggesting that the anti-neuroinflammatory mechanism of 3′-deoxyadenosin on aberrant synaptic plasticity reduces methamphetamine-induced seeking behavior. Taken together, 3′-deoxyadenosin alleviates methamphetamine-induced aberrant synaptic plasticity and seeking behavior by inhibiting the NLRP3 inflammasome.
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NEW JOURNAL OF CHEMISTRY
·
2012
·Vol. 36
·期 6
·1335-1338
·
IF 2.605
Sa Huang,Jianhua Liu,Di Liu,Qinghai Yuan
We report the facile and scalable synthesis of a highly monodispersed inorganic nanostructure based on well-designed Gd(OH)3 nanorods. X-Ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), as well as energy-dispersive spectroscopy (EDS) were applied to characterize the samples, indicating that the nanomaterials with an average length of 100 nm and an average diameter of 15 nm showed a typical hexagonal phase. Biocompatibility tests were performed from in vitro cell experiments to in vivo long-term histological assessment, indicating that the Gd(OH)3 nanorods were promising for further bio-related application. Owing to the excellent paramagnetic behavior, we further investigated the capability of the nanorods as MRI contrast agent. Compared with the conventional Gd–DTPA complex, the well-designed Gd(OH)3 nanorods present a higher T1 relaxation rate, showing more potential for biomedicine applications.
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Soft Matter
·
2011
·Vol. 7
·期 11
·5200-5207
·
IF 4.457
Xi-Lian Wei,Zeng-Bin Wei,Xiu-Hong Wang,Zhong-Ni Wang,De-Zhi Sun,Jie Liu,Huai Hua Zhao
The aqueous mixtures of an anionic surfactant (sodium dodecyl benzene sulfonate, SDBS) and an ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim]BF4) within a certain range of compositions can spontaneously separate into two immiscible aqueous phases (ATPS): one surfactant-rich and the other ionic liquid-rich. The phase diagrams of the ternary system of the SDBS/[Bmim]BF4/water were drawn based on experimental data determined at 10.0, 20.0 and 30.0 °C, respectively. The borderlines of the different specific regions in the diagrams were determined using turbidity titration method. The narrow two-phase region aqueous were found to be close to the water - [Bmim]BF4 side. The formation conditions, compositions, and physicochemical properties of the aqueous two phases have been studied. The effects of temperature, surfactant and ionic liquid content on the phase separation time and phase volume ratio of the ATPS were also investigated. This phase separation phenomenon was found to be likely due to the existence of micelle aggregates with quite large size obtained by Transmission Electron Microscopy (TEM) and Steady-state Fluorescence Quenching Measurements (SFQM) methods.
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Biomedical Materials
·
2024
·Vol. 19
·期 5
·055026
·
IF 3.9
Yu Yan,Aijia You,Xianxian Chen,Wenyi Huang,Xiaoting Lu,Cuijin Gu,Sen Ye,Jun Zhong,Haiting Huang,Yao Zhao,Yu Li,Chun Li
(+)4-cholesten-3-one has been proved to have potential wound healing effect in the process of wound regeneration. This study aimed to evaluate the effect of (+)4-cholesten-3-one/sodium alginate/gelatin on skin injury and reveal its potential molecular mechanism. First, we prepared sodium alginate/gelatin hydrogel (SA/Gel hydrogel) with different ratios and tested their characteristics. Based on these results, different concentrations of (+)4-cholesten-3-one were added into SA/Gel hydrogel. A full-thickness skin injury model was successfully established to evaluate wound healing activity in vivo. HE staining and Masson staining were used to evaluate the thickness of granulation tissue and collagen deposition level. Immunohistochemical staining and immunofluorescence staining were applied to detect the level of revascularization and proliferation in each group of wounds. Western blot, quantitative-PCR and immunofluorescence staining were used to detect the expression of proteins related to Wnt/β-catenin signaling pathway in each group of wounds. In vitro results showed that the hydrogel not only created a 3D structure for cell adhesion and growth, but also exhibited good swelling ability, excellent degradability and favorable bio-compatibility. Most importantly, in vivo experiments further indicated that (+)4-cholesten-3-one/SA/Gel hydrogel effectively enhanced wound healing. The effectiveness is due to its superior abilities in accelerating healing process, granulation tissue regeneration, collagen deposition, promoting angiogenesis, tissue proliferation, as well as fibroblast activation and differentiation. The underlying mechanism was related to the Wnt/β-catenin signaling pathway. This study highlighted that (+)4-cholesten-3-one/SA/Gel hydrogel holds promise as a wound healing dressing in future clinical applications.
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CHEMICAL ENGINEERING SCIENCE
·
2024
·
IF 4.1
Lei Wu,Menghan Liu,Yuting Chen,Ming Li,Tingting Yin,Cong Ma,Wei Fan
UF is widely used as a wastewater treatment and recycling technology. However, membrane fouling is still an important factor affecting the wide application. In this study, APIm with positive amino group was adsorbed to the surface of the carboxylated polyacrylonitrile (PAN) membrane by electrostatic force to reduce the fouling of sodium alginate (SA) caused by Ca 2+ . The results show that 1 v/v% APIm was selected as the optimal dosage, accompanied by the highest water flux of 330.5 L·m -2 h −1 bar −1 and bovine serum albumin (BSA) rejection rate of 91.0 %. When the concentration of Ca 2+ was increased to 10 mM, the Ca 2+ catch amount increased to 48.25 mg/cm 2 . The main anti-fouling mechanism was that APIm captured a certain amount of Ca 2+ in situ, destroyed the complex structure of sodium alginate (SA) and Ca 2+ . Then the fouling layer was easy to be removed by physical cleaning. At the concentration of 10 mM Ca 2+ , the membrane flux recovery rate of 1 v/v% APIm membrane was increased to 80.1 %. This study demonstrates a fouling control strategy for in-situ removal of Ca 2+ bound SA fouling, providing a new approach for the future development of UF membrane.
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高影响因子
Crystals
·
2024
·Vol. 15
·期 1
·31
·
IF 2.4
Zhongran Yao,Fen Qi,Qiang Sun,Lin Ye,Xiaowei Yang,Guojie Chao,Pei Tang,Kongjun Zhu
Lithium-conducting NASICON materials have emerged as a promising alternative to organic liquid electrolytes for high-energy-density Li-metal batteries, owing to their superior ionic conductivity and excellent air stability. However, their practical application is hindered by poor sintering characteristics and high grain boundary resistance. In this investigation, Li1.3Al0.3−xYxTi1.7(PO4)3(LAYTP-x,x= 0.00, 0.01, 0.03, 0.05, and 0.07) were successfully synthesized via conventional solid-state reaction to explore the impact of Y3+on both ionic conductivity and chemical stability. The structural, morphological, and transport properties of the samples were comprehensively characterized in order to identify the optimal doping concentration. All samples exhibited a NASICON structure with a uniform distribution of Y elements within the electrolyte. Due to its highest relative density (95.8%), the LAYTP-0.03 electrolyte demonstrated the highest total conductivity of 2.03 × 10−4S cm−1with a relatively low activation energy of 0.33 eV, making it suitable for solid-state batteries. When paired with the NCM811 cathode, the Li/LAYTP-0.03/NCM811 cell exhibited outstanding electrochemical performance: a high capacity of 155 mAh/g was achieved at 0.2C after 50 cycles with a Coulombic efficiency of approximately 100%, indicating highly reversible lithium plating/stripping facilitated by the LAYTP-0.03 electrolyte. These results suggest that the LAYTP-0.03 ceramic electrolyte could be a promising alternative for developing safe solid-state Li-metal batteries.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiwen Tao,Tongliang Bu,Qiqi Xie,Yujie Jia,Qing Wang,Nayu Shen,Xinyu Zhang,Yirong Xiao,Lin Ye,Zhao Chen,Qingfeng Li,Zizhong Tang
Resistant starch (RS) has garnered significant attention for its health benefits and controllable processing safety. In this study, buckwheat type 3 resistant starch (RS3) was prepared using autoclaving (A-BRS), microwaving (M-BRS), and autoclaving combined with enzyme treatment (AE-BRS). The physicochemical structures and functional properties of the modified starches were compared. Results showed that, compared to native buckwheat starch, the particle size volume mean diameter (VMD) of BRS increased significantly from 9.67 μm to 54.21–59.81 μm, with a crystalline structure transformation from A-type to B+V-type and an increase in gelatinization enthalpy ( ΔH ) to 3.26–7.26 J/g. Among the three resistant starches, A-BRS exhibited the largest granule size, highest water-holding capacity, and greatest transparency, while M-BRS had the roughest surface. Notably, AE-BRS, with an amylose content increased to 34.71%, exhibited enhanced relative crystallinity and short-range ordered structure, leading to higher solubility and thermal stability, with an RS content reaching 21.01%. The composite preparation of resistant starch is anticipated to become a significant trend in the future. These findings offer valuable insights for the industrial development and production of buckwheat RS3.
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危险品化学品经营许可证（带存储）