CHEMICO-BIOLOGICAL INTERACTIONS
·
2020
·
IF 3.723
Xiao-Peng Wang,Chun-Min Ma,Xin-Huai Zhao
The caseinate and glycated caseinate generated from the transglutaminase-catalyzed reaction of caseinate and oligochitosan were digested using pepsin and trypsin, and the activity of the resultant digests was measured in rat intestinal epithelial cell line (IEC-6) using several biological responses as indicators. Compared with the caseinate digest, the glycated caseinate digest had similar contents in 17 amino acids but less reactable –NH 2 contents, and 6.57 g glucosamine per kg protein; moreover, it showed higher activity in the cells ( P < 0.05) to promote cell growth, accumulate the cell-cycle progression at the S-phase, and prevent the camptothecin-induced cell apoptosis . The glycated caseinate digest also showed higher differentiation activity in the cells than the caseinate digest, resulting in enhanced activities of the three brush-border membrane enzymes ( P < 0.05) and increased microvilli on the cell surfaces. The real-time reverse transcription-polymerase chain reaction, Western-blot assay, and Dickkopf-1 (a receptor inhibitor of the Wnt/β-catenin signaling pathway) were used to determine both gene and protein expression changes in the cells. A Wnt/β-catenin signaling pathway responsible for these enhanced effects was proposed because the five genes (glycogen synthase kinase 3β, Wnt3a, β-catenin, c-Myc, and cyclin D1) and three proteins (nuclear and cytosolic β-catenin, cyclin D1 , and c-Myc) as part of this signaling pathway were regulated in the treated cells. The oligochitosan glycation of caseinate induced by transglutaminase is thus suggested endowing the peptic-tryptic caseinate digest with higher activity in the cells through its effects on the Wnt/β-catenin signaling pathway.
✓ 已验证
DOI
PubMed
高影响因子
Neuropsychiatric Disease and Treatment
·
2022
·
IF 2.989
Yuxi Jia,Ping Li,Fei Wu,Fanjie Zeng,Beilin Zhang,Jinpeng Wang
Background: In clinic, valproic acid (VPA) has been widely used in the treatments of epilepsy and is currently under clinical trials for the treatments of multiple diseases. However, gravidas receiving VPA treatment during their early stage of pregnancy were reported to be associated with elevated incidences of autism in their child. In this study, the potential influences of n-3/n-6 polyunsaturated fatty acids (PUFAs) as supplements were investigated in VPA-induced autistic rat model.Materials and Methods: A rat model of autism spectrum disorder (ASD) was established by VPA exposure at the E12.5 day of gestation. Neonatal male Wistar rats (n = 24) were divided into VPA group, A group and B group, while normal neonatal male Wistar rats served as controls. At P21 days, animals in the control and VPA groups were fed with standard chow, while rats in the A group were fed with n-3 PUFA free chow, and those in the B group with n-3/n-6 PUFA (1:5) chow. The duration of the diet was from P21 days to 3 months. All rats were subjected to the examinations of repetitive stereotyped behaviors and changes in social interaction at the end of supplement. The neuroinflammation in the brain and serum was also evaluated. Hematoxylin–eosin (HE) staining and Nissl staining were used to observe the neuronal injury in hippocampus.Results: In the B group, the repetitive stereotyped behaviors reduced, the social communication was improved, the neuronal loss and proinflammatory cytokines in the hippocampus reduced, and the extracellular STAT3 activation was inhibited in the hippocampus, as compared to the B group.Conclusion: Supplements with n-3/n-6 PUFA (1:5) rescues repetitive stereotyped behaviors and social deficits in neonatal rats exposed to VPA, likely due to the improved inflammatory responses through the alteration of STAT3 signaling. This study provides evidence for the use of n-3/n-6 PUF in the treatment of autism in humans.
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Tong Dou,Junzheng Zhang,Wenzhen Yang,ShuaiTian Guo,Meng Zhang,Jingyi Wang,Zhizhen Xu,Dongsheng Wang
Methadone and its metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP) are key biomarkers in wastewater-based epidemiology studies for assessing illicit opioid consumption. Due to their extremely low concentrations in wastewater, conventional laboratory-based detection methods are time-consuming, complex, and require expensive instrumentation, making them unsuitable for rapid on-site analysis. Herein, we developed a signal-amplified molecularly imprinted electrochemical sensor based on a Cu 2 Fe(CN) 6 /Cu-benzenehexathiol conductive metal-organic framework (CB-MOF) composite for the ultrasensitive detection of methadone and EDDP. The composite exhibits a high electrochemical signal through in situ synthesis of a conductive MOF with an integrated Prussian-Blue-analog redox probe. Guided by density functional theory (DFT) and molecular dynamics simulations, p-aminobenzoic acid (PABA) was selected as the functional monomer to construct a molecularly imprinted recognition layer for specific target binding. The sensor demonstrated a wide linear detection range for EDDP from 2.78 ng/L to 277.4 mg L −1 and methadone from 309.5 ng/L to 309.5 mg L −1 , with limits of detection as low as 99.86 pg/L and 3.28 ng/L, respectively. In addition, the sensor exhibited excellent stability, selectivity, reproducibility, regenerability, and applicability, offering robust support for the rapid on-site detection of opioids in municipal wastewater and river water.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Qinqin Chen,Xing Jin,Qifan Zheng,Min He,Liqiao Han,Jun Yan,Min Zhan,Pengwei Zhang,Peifeng Ke,Jianbing Wang,Xianzhang Huang,Qiaoxuan Zhang
High-affinity anti-double-stranded DNA (anti-dsDNA) antibodies serve as essential biomarkers for systemic lupus erythematosus (SLE), exhibiting particularly strong correlations with active disease manifestations and renal pathology. However, conventional detection methods require urea treatment to quantify these antibodies, necessitated by the heterogeneity of endogenous antibodies, which complicates assay procedures and compromises accuracy. In this study, a novel ratiometric electrochemical (EC) biosensor for the direct detection of high-affinity anti-dsDNA antibodies in serum without additional sample processing has been developed. This biosensor conducted by a novel T(6–4)T dimer dsDNA capture probe and integrated with a self-assembled 4-mercaptophenylboronic acid@gold nanoparticles@6-ferrocenyl-1-hexanethiol (4-MPBA@AuNPs@6-(Fc)HT) composite signal tag. Leveraging the highly specific of the T(6–4)T dimer dsDNA capture probe and multi-amplification capability of 4-MPBA@AuNPs@6-(Fc)HT, the platform exhibited non-specific interferences, higher sensitivity (limit of detection:1.60 IU/mL), and efficient preparation. Clinical serum analysis showed good correlation with indirect immunofluorescence (IIF) (r = 0.629, p < 0.001), superior diagnostic performance (AUC = 0.893 vs. IIF AUC = 0.900) in differentiating SLE patient from healthy control (CON). Notably, EC results correlated more strongly with renal impairment (r = 0.426, p < 0.01) than IIF (r = 0.321, p > 0.05), highlighting its superior clinical utility for monitoring disease. This strategy provides a robust and straightforward approach for serum high-affinity anti-dsDNA antibodies detection, offering significant potential for SLE diagnosis and progression monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Xiao-Tong Luo,Bao-Dong Cai,Han-Peng Jiang,Hua-Ming Xiao,Bi-Feng Yuan,Yu-Qi Feng
Trehalose-6-phosphate (T6P) is an important signaling metabolite that is involved in many physiological processes. However, the mechanism of the biological functions of T6P is not fully understood. Quantification of T6P in plants will be beneficial to elucidate the mechanism. However, it is still a challenge to chromatographically separate and sensitively detect T6P and related sugar phosphates. In the current study, we developed a method for effective separation and sensitive detection of glucose-1-phosphate (G1P), glucose-6-phosphate (G6P), sucrose-6-phosphate (S6P) and T6P in plant tissues by chemical derivatization combined with hydrophilic interaction liquid chromatography-tandem mass spectrometry (ChD-HILIC-MS/MS). With this method, two pairs of isomers (G1P/G6P and S6P/T6P) could be well separated on a HILIC column and sensitively detected by MS with limits of detection (LODs) ranging from 0.1 to 0.6 ng mL −1 . The developed method was successfully applied to the detection of endogenous G1P, G6P, S6P and T6P in small amounts of plant tissues, such as 1 mg fresh weight of Oryza sativa shoot.
✓ 已验证
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Fan Wang,Yuqing Wang,Lili Zhang,Ting Zheng,Jun Shen
Two novel cellulose phenylcarbamate derivatives bearing 3,5-dimethylphenylcarbamate substituents at 2,3-positions and 4-(4-(3-(2-benzo[ d ]thiazolyl)ureayl)benzyl)) ( Cel-1 ) or 4-(4-(3–4-(2-benzo[ d ]thiazolyl)phenyl)ureayl)benzyl)) ( Cel-2 ) at 6-position of glucose units were prepared. Their metal ion discrimination properties were then carefully scrutinized using 12 metal ions. Combined the 2,3-dimethylphenylcarbamates with a more bulky benzothiazolyl phenylcarbamate at 6-position, the derivative Cel-2 exhibited excellent recognition ability for both Cu 2+ and Fe 3+ , and achieved high selective detection at extremely low concentrations through fluorescence and UV–visible modes. The limit of detection of Cel-2 for the two metal ions in both modes were 2.0 μM (Cu 2+ ) and 1.0 μM (Fe 3+ ) (in fluorescence mode), 1.3 μM (Cu 2+ ) and 0.7 μM (Fe 3+ ) (in UV–visible mode), respectively, which were much lower than the maximum pollution level for copper ions (20 μM) and iron ions (5.4 μM) in the potable water. In addition, good anti-interference ability of Cel-2 was also observed for specific detection of Cu 2+ /Fe 3+ in the combined system containing other metal ions. It revealed that the obtained regioselectively substituted phenylcarbamate of cellulose possessed powerful performance for efficient detection of Cu 2+ /Fe 3+ in various circumstance via fluorescence and UV–visible modes. Furthermore, Cel-2 was also been prepared into smart ink, presenting its possible potential in the anti-counterfeiting application.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Lan Shi,Tianrui Gao,Zhipeng Zhu,Fan Yang,Bing Han,Jia Lv,Lin Tao,Yang Gao,Rong Hua,Huan Wu,Yichao Huang,Xiaojin He
Our recent large-scale population study demonstrated that several per- and polyfluoroalkyl substances (PFAS) are potential risk factors for sperm quality—with perfluorohexane sulfonate (PFHxS) and 6:2 chlorinated polyfluorinated ether sulfonate (6:2 Cl-PFESA) emerging as the strongest effect contributors to asthenospermia, yet their causal relationships remain unclear. Here, we provide in vivo experimental evidence and evaluation of the combined effects of 6:2 Cl-PFESA and PFHxS under human-relevant exposure. Male ICR mice were orally exposed to each compound alone or in combination for 35 days, covering one full spermatogenic cycle. Both single and combined exposures were associated with alterations of the male reproductive axis, including reduced serum testosterone and elevations in the gonadotropins luteinising hormone (LH) and follicle-stimulating hormone (FSH), and in sex hormone-binding globulin (SHBG). Testicular injury included disturbed seminiferous architecture, decreased sperm motility, and increased germ-cell apoptosis. Transcriptomics highlighted pathways related to cell adhesion, junctional integrity, and apoptosis, and Western blotting and TUNEL confirmed blood-testis barrier (BTB) protein disruption and increased germ-cell apoptosis. Toxic potency differed by endpoint, with endpoint-specific patterns across endocrine, BTB, and motility outcomes. Combined-effect analysis identified a positive non-additive interaction for LH, whereas most BTB-associated proteins showed no statistically significant interaction despite endpoint-specific deviations from the concentration-addition expectation. Collectively, these findings indicate that human-relevant co-exposure to 6:2 Cl-PFESA and PFHxS is associated with asthenospermia-related sperm and testicular alterations. The converging phenotypic, molecular, and functional findings support BTB impairment and germ-cell apoptosis as plausible contributing processes, yet the precise mechanisms still need further investigation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2025
·
IF 9.5
Ming Huang,Shunyan Ning,Juan Liu,Lifeng Chen,Mohammed F. Hamza,Yuezhou Wei
Ultra-high purity La2O3 (≥6 N, 99.9999%) with extremely low impurity elements is widely used in advanced optical and electrical fields and usually requires a complex purification process. In this study, a phosphonic acid functional resin (HEHEHP + TBP)/SiO2–P is prepared for the efficient removal of key impurity elements such as rare earth impurities, Fe, Th and U from La in a nitric acid medium. Batch experiments showed that in HNO3 solutions of pH ≥ 2, the removal efficiency of impurity ions by this resin was greater than 97%. The optimal separation factors (SFCe/La, SFPr/La, and SFNd/La) between light rare earths reached 7.55, 19.5, and 19.2, respectively. Advanced characterization confirmed the ion exchange and coordination mechanisms, involving the participation of the P–OH and PO groups. DFT calculations corroborated the higher affinity of the resin for U and heavy rare earth over light rare earth impurities. A process for the preparation of ultra-high purity La2O3 was proposed and verified by a continuous single column, achieving a purity increase from 99.995% to over 99.9999% with a purification ability of 180 mg La per g of resin and Th and U less than 0.05 ppm. It is worth noting that when the feed solution is only processed through a column filled with (HEHEHP + TBP)/SiO2–P (during the non-desorption step), a purified La(III) solution can be directly obtained, effectively adsorbing trace impurities. This process does not require the introduction of desorption agents, which reduces the economic cost. Compared with the multi-stage sequential purification method, it improves the production efficiency.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2022
·
IF 6.652
Yening Qiao,Jinfeng Bi,Qinqin Chen,Xinye Wu,Xinwen Jin,Min Gou,Xinrui Yang,Giorgia Purcaro
Baked red jujube is a common food ingredient used to enrich the nutritional value and enhance the aroma. DDMP (2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one) with intense bitterness and potential toxicity may be present in several baked red jujube-based products but there are no well-defined methods to measure it quickly, sensitively, and accurately. Headspace solid-phase microextraction with gas chromatography-tandem mass spectrometry (HS-SPME-GC-MS/MS) was applied for the analysis of DDMP. The standard curve was linear between the concentrations of 0.01 and 100 μg/mL, with a limit of detection at 0.1 ng/g that below the sensory threshold of DDMP (2.06 μg/g). Five cultivars of baked red jujubes from China were evaluated with the method for DDMP content (4.55–46.50 μg/g). The results indicated that jujube cultivars with a higher molar ratio of proline to glucose, lower moisture content, and stronger ability to resist water stress should be avoided from baking to modulate the formation of DDMP.
✓ 已验证
产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Chunhe Yu,Xiaohua Jiang,Dongmiao Qin,Guichun Mo,Xiangfei Zheng,Biyang Deng
S,N-Codoped carbon quantum dots (SNCQDs) with a quantum yield (QY) of 23% have been synthesized using lotus root as a carbon source and glutathione (GSH) as a nitrogen and sulfur source. The obtained SNCQDs exhibited many excellent features such as a simple preparation process, high fluorescence QY, satisfactory photostability, superior water solubility, and biocompatibility. The SNCQDs combined with Cu2+ were used as a novel off–on nanoprobe for 6-thioguanine (TG). Initial fluorescence is significantly quenched in this sensing system via electron transfer from SNCQDs to Cu2+. Fluorescence is recovered after adding TG owing to Cu2+–TG complex formation between Cu2+ and TG. A novel off–on fluorescent nanoprobe was presented for highly sensitive determination of TG. Under optimal conditions, the nanoprobe exhibited a wide linearity range of 0.005–80 μmol/L for TG and a detection limit of 1.6 nmol/L. The designed off–on nanoprobe was successfully utilized for detecting TG in plasma and urine of a leukemia patient and imaging of TG in living T24 cells.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF NEUROSCIENCE
·
2021
·
IF 2.292
Hongyan Wang,Huaiqing Gao
Aim Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive technique showing improvement effects on Parkinson’s disease (PD). Multiple microRNAs (miRs) are dys-expressed during the progression of PD. In the current study, the potential involvement of miRs in the anti-PD effects of rTMS was explored.Methods PD symptoms were induced in mice with 6-OHDA. The expression profile of miRs influenced by rTMS in PD mice was detected by microarray technique and validated by RT-qPCR detection. The PD mice were treated with rTMS and the changes in cognitive behaviors, substantia nigra neuron number, brain histology, and Aβ1-42 level were detected. The activity of miR-409-3p/PDHB axis was also detected to preliminarily explain the interaction between miRs and rTMS.Results The detection of microarray showed that 266 miRs were dys-expressed in PD mice after the administration of rTMS, including 101 up-regulated ones and 146 down-regulated ones. With validation by RT-qPCR detection, miR-409-3p was selected for subsequent assays. Administrations of rTMS improved cognitive behaviors of mice, increased neuron number, and attenuated brain injuries induced by 6-OHDA. The cerebrospinal fluid (CSF) level of Aβ1-42 was induced, while the brain level of Aβ1-42 was suppressed by rTMS. Regarding miR-409-3p/PDHB axis, rTMS administrations suppressed miR-409-3p level and increased the expression of PDHB.Conclusion Administrations of rTMS showed considerable protective effects on brain tissues against PD-induced injuries, which was associated with the changes in miR levels. The current study demonstrated that the inhibition of miR-409-3p by rTMS contributed to the attenuation of PD by inducing PDHB.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Zhuowei Shen,Kaifeng He,Mingcheng Xu,Kui Zeng,Jie Pan,Fengting Ou,Jianbiao Yao,Ruwei Wang,Su Zeng
A sensitive and specific bioanalytical method of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the quantification of 6-hydroxykynurenic acid (6-HKA) in a small quantity of rat plasma has been developed and comprehensively validated. Tolbutamide (Tol) was used as the internal standard (IS). An aliquot of 50 μL rat plasma sample was deproteinized by 150 μL methanol, and then 100 μL of its supernatant was mixed with 100 μL water after centrifugation . Chromatographic separation was performed on a ZORBAX Eclipse Plus C 18 Rapid Resolution HD column (2.1 mm × 100 mm, 1.8 μm) with a gradient mobile phase consisting of water containing 2 mM ammonium formate and methanol at a flow rate of 0.2 mL/min over a total run time of 5.0 min. The mass spectrometer was operated under multiple reactions monitoring (MRM) mode with the transitions m / z 206.1 → 160.0 for 6-HKA and m/z 269.0 → 170.0 for Tol. The linear range was 2.5–250 ng/mL with the square regression coefficient (r 2 ) of 0.997. The lower limit of quantification (LLOQ) was 2.5 ng/mL (Relative Error, RE +1.6% and RSD 4.8%, n = 5). The intra- and inter-day precision and accuracy was <13.3%. The mean recovery of 6-HKA in rat plasma was between the range of 99.3–103.4%. This method was successfully applied to study the pharmacokinetics of 6-HKA in rats after oral administration at a single dose of 20.0 mg/kg and after tail intravenous injection at a single dose of 2.0 mg/kg. Pharmacokinetic parameters bioavailability, C max, oral , T max, oral , AUC 0–24h, oral , AUC 0–∞, oral , AUC 0–24h, iv and AUC 0–∞, iv were 3.96%, 152.0 ± 85.5 ng/mL, 0.4 ± 0.1 h, 340.0 ± 136.3 ng/mL ∗ h, 369.5 ± 135.0 ng/mL ∗ h, 906.6 ± 324.1 ng/mL*h and 932.9 ± 336.5 ng/mL ∗ h, respectively.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）