BIOMATERIALS
·
2022
·
IF 15.304
Bin Liu,Yulong Bian,Meng Yuan,Yanlin Zhu,Shikai Liu,He Ding,Shili Gai,Piaoping Yang,Ziyong Cheng,Jun Lin
The appropriate design of multifunctional nanocarriers for chloroperoxidase (CPO) delivery and the simultaneous improvement of the efficiency of enzyme dynamic therapy (EDT) remain significant challenges. Herein, we report a facile one-step route to obtain a multifunctional nanocarrier for the formation of sodium hyaluronate-modified hollow calcium peroxide spheres with encapsulated L-buthionine sulfoximine (BSO), followed by delivery of CPO for enhanced EDT. After effective accumulation at the tumor sites, the nanocomposite rapidly decomposes and releases Ca 2+ , BSO molecules, CPO, and concurrently generates a large volume of hydrogen peroxide (H 2 O 2 ) in the endogenous tumor microenvironment (TME). BSO molecules inhibit the biosynthesis of glutathione (GSH) by inactivating γ-glutamyl cysteine synthetase. Due to BSO-induced GSH depletion and self-supply of H 2 O 2 , the EDT efficiency of CPO was significantly enhanced to achieve high tumor therapy efficiency. Additionally, overloaded Ca 2+ caused mitochondrial damage and amplified the oxidative stress . Moreover, calcification resulted from the unbalanced calcium transport channel caused by enhanced oxidative stress, accelerating tumor apoptosis and improving the efficacy of computed tomography (CT) imaging visual tumor therapy. This simple and efficient design for multifunctional nanocomposites will likely take an important place in the field of combined tumor therapeutics.
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高影响因子
Horticulture Research
·
2022
·
IF 7.291
Xie Dong-Ling,Huang Hua-Min,Zhou Can-Yu,Liu Chen-Xu,Kanwar Mukesh Kumar,Qi Zhen-Yu,Zhou Jie
The heat shock transcription factors (Hsfs) play critical roles in plant responses to abiotic stresses. However, the mechanism of Hsfs in the regulation of pollen thermotolerance and their specific biological functions and signaling remain unclear. Herein, we demonstrate that HsfA1a played a key role in tomato pollen thermotolerance. Pollen thermotolerance was reduced in hsfA1a mutants but was increased by hsfA1a overexpression, based on pollen viability and germination. Analyzing the whole transcriptome by RNA-seq data, we found that HsfA1a mainly regulated the genes involved in oxidative stress protection, protein homeostasis regulation and protein modification, as well as the response to biological stress in anthers under heat stress. The accumulation of reactive oxygen species in anthers was enhanced in hsfA1a mutants but decreased in HsfA1a-overexpressing lines. Furthermore, HsfA1a bound to the promoter region of genes involved in redox regulation (Cu/Zn-SOD, GST8, and MDAR1), protein repair (HSP17.6A, HSP70-2, HSP90-2, and HSP101) and degradation (UBP5, UBP18, RPN10a, and ATG10) and regulated the expression of these genes in tomato anthers under heat stress. Our findings suggest that HsfA1a maintains pollen thermotolerance and cellular homeostasis by enhancing antioxidant capacity and protein repair and degradation, ultimately improving pollen viability and fertility.
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高影响因子
STARCH-STARKE
·
2025
·
IF 2.7
Yanqu Cai,Haotian Chen,Yao Wen,Haiyun Chen
To contribute to the development of natural plants in the field of medicine, the study investigates optimization of the best process of extracting polysaccharides of Eleusine indica (L.) Gaertn polysaccharide (EIGP) with water as a solvent and the bioactivity of EIGP in vitro. EIGPs were extracted by an ultrasonic-assisted method using water as a solvent; a one-factor experiment was carried out first, and then a response surface experiment was designed according to the four-factor and three-level design by using the Design-Expert software. The polysaccharide extract of Eleusine indica (L.) Gaertn (EIG) was eluted with a DEAE column and Sephadex G-75 column to obtain the purity of polysaccharides. The purified polysaccharide was then subjected to preliminary antioxidant and hypoglycemic activities by enzyme inhibition and in vitro free radical scavenging assays. The optimal extraction conditions were ultrasonic power of 240 W, solid–liquid ratio of 1:17 g/mL, time of 31 min, and temperature of 50°C, under which the polysaccharide extraction rate of EIG was 11.50% and the purity was 73.45%. In the bioactivity study of polysaccharides, the results were as follows: in the α-glucosidase inhibitory activity assay, the IC 50 of these polysaccharides and acarbose were measured to be 59.84 and 4.4 × 10 −4 mg/mL, respectively; in the α-amylase inhibitory activity assay, the IC 50 of these polysaccharides and acarbose were measured to be 59.98 and 0.2173 mg/mL; in the DPPH• radical scavenging activity assay, the IC 50 of the polysaccharide and Vitamin C (VitC) were measured to be 43.95 and 0.0126 mg/mL, respectively; in the ABTS• + scavenging assay, the IC 50 of the polysaccharide and VitC were measured to be 0.4188 and 0.004 mg/mL, respectively. In this thesis, the process of extracting polysaccharides from EIG with water as a solvent was investigated and optimized. The bioactivity study experiments showed that EIGPs-1, purified from EIG, have certain antioxidant and hypoglycemic activity.
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APPLIED OPTICS
·
2019
·Vol. 58
·期 31
·8583-8588
·
IF 1.973
Suyi Zhong,Tian Guan,Yang Xu,Chongqi Zhou,Lixuan Shi,Cuixia Guo,Xuesi Zhou,Zhangyan Li,Yonghong He,Yanhong Ji
The ability to identify L(+)-ascorbic acid from D(-)-isoascorbic acid in medicinal products is of practical interest. Based on the method of frequency domain weak measurement, a set of common optical path sensors for identification of L(+)-ascorbic acid and D(-)-isoascorbic acid is established. By quantificationally analyzing the magnitude and offset direction of the spectral central wavelength, a good identification of the concentration and the optically active forms of ascorbic acid has been achieved. The sensitivity and resolution of the sensor for optical rotation can reach 34.35 nm/° and ${5.53} \times {{10}^{ - 5}}^\circ $, respectively. The detection resolution for L(+)-ascorbic acid is ${2.00} \times {{10}^{ - 4}}\;{\rm mol}/{\rm mL}$, and that for D(-)-isoascorbic acid is ${2.73} \times {{10}^{ - 4}}\;{\rm mol}/{\rm mL}$. The potential of the sensor in the detection of transparent but optically inactive impurities has been verified by comparative experiments of sodium chloride solution. The sensor also has been applied to identify medicinal vitamin C tablets, which verified the feasibility of the method in optically active pharmaceutical solutions with water-insoluble, optically inactive impurities. Since the sensor has the advantages of high precision, real-time, high robustness, and being non-destructive, it has a great prospect in the field of drug detection containing chiral molecules.
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高影响因子
Nanomaterials
·
2016
·Vol. 6
·期 10
·178
·
IF 2.69
Yuehua Zhang,Wu Lei,Yujuan Xu,Xifeng Xia,Qingli Hao
A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-modified GCE (PLL/GCE), GO-modified GCE and bare GCE, the PLL/GO/GCE exhibited an increase in the anodic potential difference and a remarkable enhancement in the current responses for both UA and DA. For the simultaneous detection of DA and UA, the detection limits of 0.021 and 0.074 μM were obtained, while 0.031 and 0.018 μM were obtained as the detection limits for the selective detection of UA and DA, using DPV in the linear concentration ranges of 0.5 to 20.0 and 0.5 to 35 μM, respectively. In addition, the PLL/GO/GCE demonstrated good reproducibility, long-term stability, excellent selectivity and negligible interference of ascorbic acid (AA). The proposed modified electrode was successfully implemented in the simultaneous detection of DA and UA in human blood serum, urine and dopamine hydrochloride injection with satisfactory results.
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高影响因子
ACS Biomaterials Science & Engineering
·
2020
·
IF 4.152
Hongjie Zong,Bo Wang,Guifei Li,Shifeng Yan,Kunxi Zhang,Yufeng Shou,Jingbo Yin
Currently, biodegradable hydrogels are one of the most promising materials in tissue engineering. From the perspective of clinical needs, hydrogels with high strength and minimally invasive implantation are preferred for the reconstruction of load-bearing tissues. In this work, a biodegradable, high-strength, and injectable hydrogel was developed by one-step photo-cross-linking of two biomacromolecules, polyethylene glycol acrylated poly(l-glutamic acid) (PLGA-APEG) and methacrylated gellan gum (GG-MA). The hydrogels, named as PLGA/GG hydrogels, exhibited high mechanical properties. The compression stress of the hydrogels was 0.53 MPa, and the fracture energy was 7.7 ± 0.2 kJ m–2. Meanwhile, the storage modulus could reach 44.0 ± 0.6 kPa. The hydrogel precursor solution loaded with adipose-derived stem cells (ASCs) was subcutaneously injected into C57BL/6 mice and then cross-linked via in situ transdermal irradiation, which demonstrated the excellent injectability and subcutaneous gelatinization of PLGA/GG hydrogels as cell carriers. Furthermore, three-dimensional encapsulation of ASCs in hydrogels after 7, 14, and 21 days showed outstanding cytocompatibility, and the viability of ASCs was up to 84.0 ± 1.7%. The PLGA/GG hydrogels exhibited ideal behaviors of degradation, with 60 ± 5% of hydrogels degraded in phosphate buffered solution (PBS) after 11 weeks. H&E staining demonstrated that the hydrogels degraded gradually after 6 weeks and supported tissue invasion without inflammatory reactions, which indicated the laudable biodegradability of hydrogels. Hence, the biodegradable and high-strength hydrogels with well-performed injectability and biocompatibility possessed high potential applications in tissue engineering, especially in load-bearing tissue regeneration.
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高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 18
·7981-7997
·
IF 3.553
Zhang Xiaoyong,Liu Huihui,Yue Lipei,Bai Yongping,He Jinmei
When it comes to bonding in an aqueous environment, intertidal marine organisms such as mussels, sandcastle worms, barnacles and polychaetes are providing inspiration for the assembly of synthetic molecules into the polymeric adhesives. Although intense demands in various life or industrial applications and many mussel-inspired systems have been shown to develop coatings and hydrogels, exploring the mimetic mussel adhesive system through the control of composition and polymer molecular weight is demonstrated less often. In this report, we showed a simple approach to synthesize mussel-inspired adhesive, P( N -butyl acrylate- co - l -Dopa-methacrylate anhydride), which possesses characteristics of simple synthesis, easy-using, no extra-crosslinker need, and excellent adhesion behaviour on diverse substrates. Adhesion behaviour was examined using low- and high-surface-energy substrates (e.g. PTFE and steel, respectively). Furthermore, the fabricated adhesives exhibited excellent underwater adhesive behaviour for steel in various aqueous solutions, including ion ((IO 4 ) − and Mn 3+ ), acid and alkali solutions (pH = 4, 6.7 and 8, respectively). The outstanding fabricated adhesives take advantage of the catechol functionality and wettability of the resulting polymer. These insights should help us to design next-generation underwater adhesive systems as well as open up a number of future research designs for mussel-inspired adhesives with performance not accessed previously.
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RSC Advances
·
2022
·Vol. 12
·期 10
·6063-6075
·
IF 4.036
Gao Lidi,Hu Xingfang,Qin Shili,Chu Hongtao,Zhao Xuan,Wang Bingbing
A new kind of chiral zirconium based metal–organic framework, L-Cys-PCN-222, was synthesized using L-cysteine (L-Cys) as a chiral modifier by a solvent-assisted ligand incorporation approach and utilized as the chiral stationary phase in the capillary electrochromatography system. L-Cys-PCN-222 was characterized by X-ray diffraction, thermogravimetric analysis, X-ray photoelectron spectroscopy, Fourier-transform infrared spectra, nitrogen adsorption/desorption, circular dichroism spectrum, zeta-potential and so on. The results revealed that L-Cys-PCN-222 had the advantages of good crystallinity, high specific surface area (1818 m2 g−1), thermal stability and chiral recognition performance. Meanwhile, the L-Cys-PCN-222-bonded open-tubular column was prepared using L-Cys-PCN-222 particles as the solid phase by ‘thiol–ene’ click chemistry reaction and characterized by scanning electron microscopy, which proved the successful bonding of L-Cys-PCN-222 to the column inner wall. Finally, the stability, reproducibility and chiral separation performance of the L-Cys-PCN-222-bonded OT column were measured. Relative standard deviations (RSD) of the column efficiencies for run-to-run, day-to-day, column-to-column and runs were 1.39–6.62%, and did not obviously change after 200 runs. The enantiomeric separation of 17 kinds of chiral compounds including acidic, neutral and basic amino acids, imidazolinone and aryloxyphenoxypropionic pesticides, and fluoroquinolones were achieved in the L-Cys-PCN-222-bonded OT column. These results demonstrated that the chiral separation system of the chiral metal–organic frameworks (CMOFs) coupled with capillary electrochromatography has good application prospects.
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高影响因子
CURRENT ORGANIC SYNTHESIS
·
2024
·Vol. 21
·期 1
·47-60
·
IF 1.7
Zhang Cuizhi,Fan Yanchao,Wang Sijia,Liu Shaojie,Chu Xiaomeng,Tang Erjun
Introduction: L-proline is an efficient chiral small-molecule organocatalyst for the direct asymmetric aldol reaction between unmodified acetone and a variety of aldehydes. Methods: However, it is difficult to separate from the reaction medium for reuse. In this work, polyacrylic
acid (PAA) supported The acylation reaction between L-hydroxyproline and PAA prepared lproline (P(AA-co-PA)) catalysts with various catalyst loadings. Fourier characterized them transforms infrared spectroscopy, nuclear magnetic resonance spectrum, gel permeation chromatography and thermogravimetry
analysis. Results: These macromolecular catalysts were used to catalyze acetone and benzaldehydes' direct asymmetric aldol reaction. The influence of the catalyst structure on the catalytic performance was studied, and the reaction conditions were optimized. Conclusion: The results
showed that P(AA-co-PA) with 50 mol% catalyst loading had excellent catalytic performance, much higher than that of L-proline and L-hydroxyproline. Its recovery was achieved by simple filtration. After being reused 7 times, its catalytic performance was still higher than that of L-proline.
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高影响因子
Analytical Methods
·
2025
·
IF 2.6
Minzi Li,Hongying Wang,Shaohong Zhang,Ran Zhang,Zhijuan Wang
A trimetallic (Fe, Co, Zn) nanozyme embedded within a N-doped carbon matrix (FeCoZn-NC-1.5-800) was engineered via coordination-controlled pyrolysis of Fe ions doped core-shell ZIF-8@ZIF-67 (ZIF-8@ZIF-67-Fe-1.5) precursor, which exhibited dual oxidase/peroxidase-mimicking activities. The bifunctional catalyst oxidized 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue product of oxidized TMB (oxTMB) without exogenous H2O2. However, the addition of L-cysteine (L-Cys) acted as a potent inhibitor, quenching oxTMB formation via thiol-mediated electron transfer, leading to a significant decrease in absorbance and the fading of the blue colour to colourless. Consequently, an H2O2-independent colormetric biosensor was developed with a wide linear range (5-50 μM), a low detection limit (0.569 μM, 3σ), good selectivity and anti-interference ability. This work establishes a paradigm for transition metal nanozyme design, offering clinical translation potential in point-of-care thiol monitoring and biocatalytic system development.
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高影响因子
BIOORGANIC CHEMISTRY
·
2024
·
IF 4.5
Xiaojiang Wang,Yan Lu,Meixia Li,Xiao Xia,Can Jin,Kan Ding,Daofeng Chen
Commelina communis L., a functional food and herbal plant in Asia, has been used against obesity, diabetes, and infections for centuries. A growing body of studies has demonstrated that indigestible polysaccharides are significant in obesity management. However, the structures and bioactivities of homogeneous polysaccharides from C. communis remain unclear. This study presented the structural characterization, simulated digestion, and human gut Bacteroides proliferation promotion activity of a novel homogeneous polysaccharide (CCB-3) from C. communis . The results showed that CCB-3 was an arabinoglucuronoxylan, primarily composed of arabinose, galactose, xylose, glucuronic acid (GlcA), and 4- O -methyl GlcA with a molecular weight ( Mw ) of 58.8 kDa. Following a 6-hour exposure to simulated gastrointestinal fluid, the Mw of CCB-3 remained unchanged, revealing that CCB-3 was an indigestible polysaccharide. Notably, CCB-3 could promote the proliferation of B. thetaiotaomicron, B . ovatus , and B . cellulosilyticus and produce short-chain fatty acids (SCFAs) and 1,2-propanediol. These findings might shed light on the discovery of polysaccharide-based leading compounds from C. communis against obesity.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Peng Zhu,Jianmeng Wu,Zhipeng Chang,Feng Yang,Xinqun Zhang,Ke Hou,Dehai Ping,Songjie Li
Novel composite hydrogels composed of Capparis spinosa L. extract (CSL) and sodium alginate (SA) were developed for biomedical applications using calcium chloride (CaCl₂) as a nontoxic ionic crosslinker. The swelling degree, antioxidant activity, water retention, and biocompatibility of the CSL/SA composite hydrogels were thoroughly analyzed, along with their antibacterial properties. Scanning electron microscopy (SEM) results indicated that the CSL/SA composite hydrogels exhibited a three-dimensional porous structure with uniform pore distribution. Fourier transform infrared spectroscopy (FTIR) results suggested that CSL was successfully incorporated into composite hydrogels. When the CSL concentration reached 9 %, the swelling degree attained 765.89 ± 21.36 %. Furthermore, the addition of CSL enhanced the oxidation resistance of the composite hydrogels. Agar disk diffusion assessments confirmed that the CSL/SA composite hydrogels exhibited significant antibacterial effects against E. coli and S. aureus. Cytotoxicity studies demonstrated that the composite hydrogels effectively accelerated cell proliferation. Therefore, these hydrogels show promising potential for application as wound dressings.
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