Food & Function
·
2018
·Vol. 9
·期 9
·5007-5015
·
IF 3.289
Zheng-Nan Cai,Wan Li,Shomaila Mehmood,Wen-Juan Pan,Qing-Xi Wu,Yan Chen,Yong-Ming Lu
Polysaccharides from Morchella esculenta are known to exhibit diverse bioactivities, while an anti-melanogenesis effect has been barely addressed. Herein, the anti-melanogenesis activity of a heteropolysaccharide from M. esculenta (FMP-1) was investigated in vitro and in vivo. FMP-1 had no significant cytotoxic effect on B16F10 melanoma cells as well as zebrafish larvae, but did reduce melanin contents and tyrosinase activities in both of them. Treatment with FMP-1 also effectively suppressed the expression of melanogenesis-related proteins, including MC1R, MITF, TRP-1 and TRP-2, through decreasing the phosphorylation of cyclic adenosine monophosphate response element-binding protein (CREB). Moreover, the mitogen-activated protein kinase (MAPK) pathway was observed mediating FMP-1's inhibitory effect against melanin production. Specifically, FMP-1 treatment markedly inhibited the activation of phosphorylation of p38 mitogen-activated protein kinase. These results suggested that FMP-1's inhibitory effect against melanogenesis is mediated by the inhibition of CREB and p38 signaling pathways, thereby resulting in the downstream repression of melanogenesis-related proteins and the subsequent melanin production. These data provide insight into FMP-1's potential anti-melanogenesis effect in food and cosmetic industries.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Hui Gu,Dong Chen,Jia-Ping Huang,Yu-Ting Wu,Lu Hu,Shao-Liang Jiang,Jie-Hua Shi
Entrectinib (ENB) is a multi-target tyrosine kinase inhibitor currently approved for the treatment of adult and pediatric patients with NTRK fusion-positive advanced recurrent solid tumors and metastatic ROS1-mutated non-small cell lung cancer. The interaction between ENB and human α1-acid glycoprotein (HAG) was assessed by multispectral experiments and computer simulations. The results demonstrated that ENB effectively quenched HAG fluorescence via a mixed quenching mechanism. Specifically, ENB bound to the hydrophobic cavity of HAG, forming an ENB-HAG complex with 1:1 stoichiometry. The binding constant (∼10 6 M −1 ) demonstrated strong binding affinity between ENB and HAG. Non-radiative energy transfer occurred between ENB and HAG, with a binding distance of 2–4 nm. Synchronous and three-dimensional fluorescence spectroscopy indicated minimal effects on the microenvironment around the TYR and TRP residues. Circular dichroism spectroscopy demonstrated a slight alteration on the conformation of HAG, specifically a reduction in β-folded content from 43.5 % to 43.3 %. Thermodynamic calculations confirmed spontaneous binding driven primarily by hydrophobic force, which was further supported by Molecular dynamics simulationsanalyzing energy contributions of individual amino acid residues. Additionally, metal ions were found to exert varying degrees of influence on the binding process.
✓ 已验证
DOI
高影响因子
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
·
2023
·
IF 5.2
Pei Li,Zhishuang Yang,Ting Lei,Yujie Dai,Yang Zhou,Dekang Zhu,Hongyan Luo
Objectives: To elucidate the role of a novel carbapenem-hydrolysing class D β-lactamase (RAD-1) from Riemerella anatipestifer. Methods: We applied WGS and bioinformatic analysis to screen putative β-lactamase genes in R. anatipestifer SCVM0004. A putative class D β-lactamase gene was cloned into pET24a and transferred into Escherichia coli BL21 (DE3) for antibiotic susceptibility determination and protein purification. Meanwhile, the purified native protein was used to determine the enzymatic activities. Results: A class D β-lactamase, RAD-1, was identified in the genome of R. anatipestifer SCVM0004. It was distinct from all characterized class D β-lactamases (≤42% amino acid sequence identity). Searching in GenBank showed that blaRAD-1 was widely disseminated among R. anatipestifer. Genomic environment analysis indicated that the chromosomal structures of blaRAD-1-located regions were relatively conserved. Expression of RAD-1 in E. coli results in elevated MICs for various β-lactam antibiotics, including penicillins, extended-spectrum cephalosporins, a monobactam and carbapenems. Moreover, kinetic analysis of purified RAD-1 revealed: (i) high-level activity against penicillins; (ii) highest affinity for carbapenems; (iii) moderate hydrolysis of extended-spectrum cephalosporins and a monobactam; and (iv) no activity for oxacillin and cefoxitin. Conclusions: This study identified a novel chromosomally located class D carbapenemase RAD-1 (Bush-Jacoby functional group 2def) in R. anatipestifer SCVM0004. Moreover, bioinformatic analysis confirmed that the RAD-1 was widely prevalent and conserved in R. anatipestifer.
✓ 已验证
DOI
PubMed
高影响因子
ANNALS OF CLINICAL AND LABORATORY SCIENCE
·
2023
·Vol. 53
·期 1
·116-125
·
IF 0.8
Yunfang Cai,Ting Zhang,Guiying Chen,Chunxi Liu
Objective Flap endonuclease 1 (FEN1) has been confirmed to involve the drug resistance of multiple cancers including breast cancer.However, the effect of miRNA-mediated FEN1 on breast cancer cell resistance is still ambiguous and needs further research. Methods Firstly, we used GEPIA2 to predict the FEN1 expression in breast cancer. Next, we used quantitative real-time polymerasechain reaction (qRT-PCR) and western blot to evaluate the FEN1 level of cells. After parental cells or MDA-MB-231-paclitaxel(PTX) cells being transfected with or without siFEN1, the apoptosis, migration, and protein levels of FEN1, Bcl-2, and resistance-relatedgenes were examined by flow cytometry, wound healing assay, and western blot, respectively. Then, the putative miRNA targetingFEN1 was predicted using StarBase V3.0, and further confirmed by qRT-PCR. The targeted binding of FEN1 to miR-26a-5p was detectedby dual-luciferase reporter assay. After parental cells or MDA-MB-231-PTX cells being transfected with or without miR-26a-5pmimic, the apoptosis, migration, and protein levels of FEN1, Bcl-2, and resistance-related genes were tested again. Results FEN1 expression was enhanced in breast cancer and MDA-MB-231-PTX cells. The combined application of FEN1 knockdown and PTXenhanced apoptosis in MDA-MB-231-PTX cells but suppressed cell migration and expressions of FEN1, Bcl-2, and resistance-relatedgenes. Then, we confirmed that FEN1 was targeted by miR-26a-5p. The combined application of miR-26a-5p mimic and PTX largelyfacilitated apoptosis in MDA-MB-231-PTX cells but restrained cell migration and expressions of FEN1, Bcl-2, and resistance-relatedgenes. Conclusion MiR-26a-5p contributes to the sensitivity of breast cancer cells to paclitaxel via restraining FEN1.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2024
·
IF 3.5
Yue Xian,Yang Zhou,Jia Chen,Wenyuan Li,Ruifeng Wu,Shuangji Feng,Aimin Chang,Bo Zhang
Solid solution modulation is an ideal method for combining the advantages of different parent compounds while mitigating their disadvantages. Here, we investigated the thermal sensitivity, magnetic properties, and microwave absorption of Mg x Mn 1− x Al 2 x Fe 2(1− x ) O 4 (0.2 ≤ x ≤ 0.8) spinel solid solution ceramics for application in multifunctional devices. The electrical transport and magnetic properties can be modulated by adjusting the solution ratio. The ceramics exhibit negative temperature coefficient characteristic. The B -values from 5065–8056 K, suggesting accurate temperature measurements over a wide temperature range. As a type of soft magnetic material, it has a narrow hysteresis loop and high resistivity. Vector network analyzer studies indicate Mg 0.2 Mn 0.8 Al 0.4 Fe 1.6 O 4 could be a candidate for microwave absorption in S-band. This study successfully extends the applicability of Mg x Mn 1− x Al 2 x Fe 2(1− x ) O 4 ceramics for high-temperature thermistors and also confirms potential for multifunctional device.
✓ 已验证
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047509
·
IF 3.9
Mengli Wang,Lele Ma,Le Kang,Mingsan Miao,Huaixia Yang
Estrogen receptor alpha (ERα) is an important biomarker for breast cancer which is positive in over 75% of breast cancer cases. In this study, an electrochemical biosensor was constructed for sensitive detection of ERα based on the signal amplification of AuNPs-SWCNT nanocomposites and hemin@HKUST-1. AuNPs-SWCNT was served as a conductive substrate, and be used to immobilize the aptamer (Apt) by an Au–S bonding. Then, Apt, ERα and antibody (Ab) formed a sandwich composite structure through specific recognition and immobilized on the electrode. The hemin@HKUST-1 was introduced into the biosensor via an amide bond with Ab and acted as the signal molecule. AuNPs-SWCNT could significantly enhance the conductivity of the electrode and the large specific surface area of HKUST-1 enabled it to load more hemin, and greatly improved the sensitivity of the biosensor. The detection limit (LOD) of the biosensor could reach 27.8 fg·ml−1 after optimization of the conditions, with the current intensity and the logarithm of the ERα concentration exhibited a good linear relationship between 100 fg·ml−1 and 100 ng·ml−1. In addition, the biosensor possessed excellent selectivity, stability, reproducibility and Interference immunity. More importantly, it shows great application potential in practical detection.
✓ 已验证
DOI
高影响因子
CELLULAR AND MOLECULAR LIFE SCIENCES
·
2024
·Vol. 81
·期 1
·1-22
·
IF 6.2
Feng Chenxi,Yan Jinhua,Luo Ting,Zhang Hong,Zhang Hu,Yuan Yu,Chen Yi,Chen Haiyang
Inflammatory bowel diseases (IBDs) are immune chronic diseases characterized by recurrent episodes, resulting in continuous intestinal barrier damage and intestinal microbiota dysbiosis. Safe strategies aimed at stabilizing and reducing IBDs recurrence have been vigorously pursued. Here, we constructed a recurrent intestinal injury Drosophila model and found that vitamin B12 (VB12), an essential co-factor for organism physiological functions, could effectively protect the intestine and reduce dextran sulfate sodium-induced intestinal barrier disruption. VB12 also alleviated microbial dysbiosis in the Drosophila model and inhibited the growth of gram-negative bacteria. We demonstrated that VB12 could mitigate intestinal damage by activating the hypoxia-inducible factor-1 signaling pathway in injured conditions, which was achieved by regulating the intestinal oxidation. In addition, we also validated the protective effect of VB12 in a murine acute colitis model. In summary, we offer new insights and implications for the potential supportive role of VB12 in the management of recurrent IBDs flare-ups.
✓ 已验证
DOI
高影响因子
POLYMER
·
2020
·
IF 4.231
Li Fan,Dongsheng Yang,Lei Huang,Mao Fan,Chuxin Lei,Qiang Fu
Polymer-based nanocomposite with high-dielectric-constant nanofillers has attracted lots of attention in dielectric material for energy storage as film capacitor. However, it is still challenging that the addition of state-of-the-art fillers always leads to electric field distortion and high leakage current resulted from poor insulation and breakdown strength of these fillers. In this study, to address this issue, a novel hybrid nanofiber with hierarchical structure was elaborately designed, which exhibits superior inherent dielectric property than conventional high-dielectric-constant fillers. The hierarchical hybrid nanofiber was fabricated by embedding boron nitride nanosheet (BNNS) into grain boundary interspace of barium titanate nanofiber to strengthen nanofiller straightforward, based on the bold conjecture that BNNS acted as solid barrier for electrical tree propagation and leakage current conduction inside nanofiller. Using hybrid nanofiber as filler, resulting nanocomposite exhibited simultaneously enhanced dielectric constant and breakdown strength and a much larger energy storage density ( U e ≈ 15.25 J cm −3 ) than conventional nanofiller-incorporated composite, which was 3.07 times of pristine matrix. The hierarchically designed hybrid nanofiber guaranteed good insulation and strong polarization of high-dielectric-constant nanofiller simultaneously and this work provides a novel and effective approach to improve the dielectric performance of composite dielectric.
✓ 已验证
DOI
高影响因子
Nano Select
·
2022
·Vol. 3
·期 10
·1395-1406
·
IF 0
Yunfei Peng,Zhenbao Xia,Yuanhang Song,Yuexia Zhang,Yanhui Li,Pengfei Zhang
This paper is dedicated to improve the conversion efficiency of carboxylation for polymers of intrinsic microporous (PIM-1). According to stable thermal properties of PIM-1, a hydrothermal method is used for the first time to achieve the carboxylation conversion of PIM-1 (C-PIM), achieving a conversion rate of more than 90% at a reaction time of 10 hours, which is nearly five times more efficient than the most efficient conversion process currently available, preserving the film-forming properties. The completion of the carboxylation conversion is verified using 1 H-NMR and 13 C-NMR, FT-IR, and organic elemental analysis. It is found that the change in molecular weight affected the film-forming properties of the membrane material, and the hydrophobicity of the C-PIM membrane did not decrease compared with C-PIM membrane reported in previous studies. The effect of C-PIM on the selectivity of CO 2 gas is explained from the microscopic to the macroscopic level by using physical characterization such as molecular structure analysis, XRD, cross-linkage calculation, pore size and volume distribution, and fractional free volume (FFV) calculation. It verifies that the efficiency of CO 2 adsorption and diffusion selectivity for PIM-1 membranes has been improved by carboxylation, preserving excellent separation, and capture capacity of CO 2 .
✓ 已验证
DOI
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Simei Sun,Chaochao Qin,Hua Liu,Chao Jiang
Anthraquinone derivatives have been widely used as photocleavage agents and dyes. Here, photoinduced excited state intramolecular charge transfer (ICT) dynamics of 1-aminoanthraquinone in ethanol are studied by femtosecond transient absorption (fs-TA) spectroscopy and quantum chemical (QC) calculations. Four decay associated difference spectra and corresponding lifetime components were obtained by singular value decomposition and global fitting analysis from the fs-TA spectra. The QC calculations prove that the S 1 state has obvious ICT character. Planar ICT (PICT) and twisted ICT (TICT) reaction coordinates are observed. On the PICT coordinate, vibrational relaxation (VR) and intersystem crossing (ISC) processes are observed. The ISC efficiency is dependent on excitation wavelength, which elucidates that T′ 2 state participates in the ISC process on a higher-level than the S′ 1 state. On the TICT coordinate, the TICT process is dependent on excitation wavelength, which elucidates a direct experimental evidence of an energy barrier in excited state TICT potential energy.
✓ 已验证
DOI
高影响因子
VETERINARY MICROBIOLOGY
·
2022
·
IF 3.246
Hongyan Luo,Mengru Li,Zhishuang Yang,Dekang Zhu,Pei Li
A novel chromosomally-located β-lactamase gene, bla RASA-1 , was identified in Riemerella anatipestifer RA-CH-1. The RASA-1, encoded by bla RASA-1 , was a class A extended-spectrum β-lactamase (ESBL), which shared 42.7% and 40.5% identities with the RAA-1 and CGA-1 β-lactamase, respectively. Overexpression of RASA-1 in Escherichia coli confers broad resistance to β-lactams and the purified native RASA-1 revealed ESBL-like hydrolysis activity. Blasting in GenBank showed that bla RASA-1 was exclusively detected in Riemerella anatipestifer . Moreover, sequence analysis revealed that this gene was located within the multi-resistance region of Riemerella anatipestifer genome.
✓ 已验证
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2017
·
IF 3.454
Yi Ding,Hui Dan,Xirui Lu,Xiaoyan Shu,Zhihao Hong,Shuai Zhang
A series of Zr 1- x Nd x O 2- x /2 (0 ≤ x ≤ 1) ceramics was prepared by solid-state reaction method. The effects of Nd content on the phase evolution were investigated. The chemical durability of resulting waste forms was also examined. The results show that the ceramics with x < 0.1 show monoclinic and cubic zirconia phase, with 0.2 ≤ x < 0.4 exhibit a single cubic phase, with 0.4 ≤ x ≤ 0.6 exhibit a single pyrochlore phase, with 0.6 < x < 0.8 exhibit a single cubic phase and remain cubic phases and hexagonal Nd 2 O 3 when 0.8 ≤ x ≤ 1. The unit cell parameters of the Nd-doped zirconia samples increase as the Nd content increases. Moreover, the normalized element release rates of Nd element in Nd-doped zirconia ceramics firstly decrease with leaching time and almost no change after 21 days (∼0 −6 g m −2 d −1 ), demonstrating its good chemical durability.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）