FOOD CHEMISTRY
·
2024
·
IF 8.5
Ying Bu,Maomei Fan,Chaonan Sun,Wenhui Zhu,Jianrong Li,Xuepeng Li,Yi Zhang
(−)-Epigallocatechin-3-gallate (EGCG) is remarkably efficacious in inhibiting the browning of red meat. We therefore propose a hypothesis that EGCG forms complexes with myoglobin , thereby stabilizing its structure and thus preventing browning. This study investigated the interaction mechanism between EGCG and myoglobin. EGCG induced static quenching of myoglobin. Noncovalent forces, including hydrogen bonds and van der Waals, primarily governing the interactions between myoglobin and EGCG. The interactions primarily disrupted myoglobin's secondary structure , thus significantly reducing surface hydrophobicity by 53% ( P < 0.05). The modification augmented the solubility and thermal stability of myoglobin. The radius of gyration (Rg) value fluctuated between 1.47 and 1.54 nm, and the hydroxyl groups in EGCG formed an average of 2.93 hydrogen bonds with myoglobin. Our findings elucidated the formation of stable myoglobin–EGCG complexes and the myoglobin–EGCG interaction, thus confirming our initial hypothesis.
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OPTICAL MATERIALS
·
2024
·
IF 3.8
Lin Chen,Xiong Shen,Jinhe Wei,Zhongming Wang,Fei Hu,Limin Bian,Qiuyun Ouyang
CsPb(Cl/Br) 3 perovskite nanocrystals (NCs) materials were synthesized by a hot injection method. To enhance the stability of the perovskite , CsPb(Cl/Br) 3 - multiwalled carbon nanotubes (CNTs) composites were synthesized by the insitu growth method. First, the successful composite of CNTs and perovskite has been proved by X-ray photoelectron spectroscopy (XPS). After CsPb(Cl/Br) 3 NCs have grew on CNTs, the exciton lifetime decays from 8.2 ns to 4.5 ns, suggesting the presence of an additional electron transfer pathway between CNTs and CsPb(Cl/Br) 3 NCs. The photoluminescence (PL) properties of perovskite NCs and CsPb(Cl/Br) 3 -CNTs were evaluated using temperature-dependent PL spectroscopy . The exciton binding energy of the CsPb(Cl/Br) 3 -CNTs composites (59.28 meV) is higher than that of the CsPb(Cl/Br) 3 NCs (51.79 meV), indicating a more stable exciton state within the CsPb(Cl/Br) 3 -CNTs structure. The PL stability experimental results reveal a notable enhancement in the stability of CsPb(Cl/Br) 3 -CNTs composites compared to CsPb(Cl/Br) 3 NCs. In addition, electrochemical tests of CsPb(Cl/Br) 3 -CNTs show a low resistance to charge transfer ( R ct ) alongside a favorable photocurrent response. This work has proved that the novel CsPb(Cl/Br) 3 -CNTs composites not only exhibit outstanding moisture and UV resistance but also demonstrate remarkable potential optoelectronic device applications.
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Materials Today Communications
·
2024
·
IF 3.7
Chunlong Qiu,Haiyang Sun,Yeyang Ni,Zhenjiang Dou,Ming Xia,Zhaoyang Fei,Zhuxiu Zhang,Mifen Cui,Qing Liu,Xu Qiao
The impressive water stability demonstrated by MIL-101(Cr) highlights its considerable potential for the effective elimination of organic contaminants from wastewater. Nevertheless, the limited availability of adsorption sites within MOFs leads to an unsatisfactory capacity for tetracycline (TC) adsorption. In this case, the lanthanum modified MIL-101(Cr) porous adsorbents, termed as nLa-MIL-101(Cr), were successfully synthesized using a post-synthetic modification method. Through various characterization analyses, it was confirmed that lanthanum exists in the form of La(OH) 3 within the adsorbents, and all adsorbents also preserved the original structure as MIL-101(Cr). The results of static adsorption experiments demonstrated that, compared to the original material, the adsorption capacity of 1La-MIL-101(Cr) for TC increased by 3.5 times. Further experiments demonstrated that the adsorbent exhibits remarkable ion interference resistance and cycle stability. Finally, based on X-ray photoelectron spectroscopy, the adsorption mechanism was primarily attributed to the coordination between TC molecules and metals active site, while electrostatic interactions also played a significant role in the process. This study has developed a highly efficient adsorbent for the treatment of TC-contaminated wastewater, which reflects promising practical application prospects.
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MOLECULES
·
2024
·Vol. 29
·期 15
·3700
·
IF 4.2
Yaliang Huang,Jiaqiang Wang,Yirui Xu,Jiwen Zhang,Ning Xia
In this work, we report on an electrochemical method for the signal-on detection of caspase-3 and the evaluation of apoptosis based on the biotinylation reaction and the signal amplification of methylene blue (MB)-loaded metal–organic frameworks (MOFs). Zr-based UiO-66-NH2MOFs were used as the nanocarriers to load electroactive MB molecules. Recombinant hexahistidine (His6)-tagged streptavidin (rSA) was attached to the MOFs through the coordination interaction between the His6tag in rSA and the metal ions on the surface of the MOFs. The acetylated peptide substrate Ac-GDEVDGGGPPPPC was immobilized on the gold electrode. In the presence of caspase-3, the peptide was specifically cleaved, leading to the release of the Ac-GDEVD sequence. A N-terminal amine group was generated and then biotinylated in the presence of biotin-NHS. Based on the strong interaction between rSA and biotin, rSA@MOF@MB was captured by the biotinylated peptide-modified electrode, producing a significantly amplified electrochemical signal. Caspase-3 was sensitively determined with a linear range from 0.1 to 25 pg/mL and a limit of detection down to 0.04 pg/mL. Further, the active caspase-3 in apoptosis inducer-treated HeLa cells was further quantified by this method. The proposed signal-on biosensor is compatible with the complex biological samples and shows great potential for apoptosis-related diagnosis and the screening of caspase-targeting drugs.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2025
·
IF 21.1
Hao Du,Bo Shen,Rubin Huo,Yuxin Hui,Chao Chen,Aoxiang Liu,Zhe Zhang,Chunjuan Li,Bingjie Ni,Derek Hao,Qi Wang
In this work, we synthesized perylene-3,4,9,10-tetracarboximide/WO 3 (PDINH/WO 3 ) with strong hetero-interfacial interactions (SHIs) to enhance the three-electron oxygen reduction reaction (3e - ORR) for hydroxyl radicals (•OH) generation toward micropollutants degradation and antibacterial applications. Experimental and density functional theory (DFT) computational results revealed that WO 3 facilitates the 2e - oxygen reduction pathway for H 2 O 2 production, while the organic PDINH component strengthens the adsorption of *H 2 O 2 intermediates, promoting their subsequent 1e - reduction to •OH. The optimized system enabled exceptional •OH production, with excellent tetracycline degradation and robust antibacterial activity, alongside 120 h stability with < 5 % activity loss in continuous-flow wastewater treatment, even under high-salinity, extreme pH, and natural organic matter interference. This work establishes SHIs as a universal design principle for steering 3e - ORR pathways. The findings offer transformative opportunities for energy-efficient environmental remediation, demonstrating exceptional resilience in complex real-world wastewater systems.
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APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Yao Yu,Zhifeng Zhang,Yijun Cao,Wei Sun,Zhiyong Gao,Haisheng Han,Pan Chen,Jian Cao
To improve the separation of ilmenite from its associated gangue mineral titanaugite through selective flotation, a novel hydroxamic acid derivative, N-hydroxy-3-(4-methoxyphenyl) acrylamide (HMPA), was successfully synthesized and evaluated as an efficient collector. Comparative flotation tests demonstrated that HMPA outperformed conventional sodium oleate, achieving superior separation efficiency with a TiO 2 concentrate grade of 48.95 % and an impressive recovery rate of 76.0 %. To understand the underlying mechanisms, the interactions between HMPA and the mineral surfaces were investigated meticulously by adsorption capacity tests, contact angle analysis, FTIR, and XPS. The results of contact angle and adsorption measurements intuitively revealed that HMPA exhibited significantly greater adsorption capacity on the ilmenite surface compared to titanaugite. FTIR and XPS analyses indicated that HMPA adsorbed strongly onto the ilmenite surface through chelation with reactive Ti and Fe sites. Density functional theory (DFT) calculations further suggested that HMPA could act as a bidentate ligand, forming a stable five-membered chelate structure through interactions with the Ti and Fe active sites on the ilmenite surface. In conclusion, HMPA demonstrated outstanding collecting ability and selectivity, making it a highly promising collector for ilmenite flotation.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 11
·e71248
·
IF 3.8
Liu Tang,Ruzhen Peng,Jingyi Guo,Chun Wang,Tianshuai Wang,Yindi Bao,Ying He
Atopic dermatitis (AD), a chronic, relapsing, inflammatory skin disease often accompanied by psychiatric comorbidities like anxiety and depression, poses significant therapeutic challenges. This study aims to explore the therapeutic effects and mechanisms of dietary supplementation with epigallocatechin‐3‐gallate (EGCG) on AD skin lesions and comorbid anxiety/depressive disorders through in vivo and in vitro experiments. Macroscopical and histopathological evaluation revealed that gavage administration of EGCG (50 or 100 mg/kg) significantly improved AD skin lesions, as evidenced by the reduced dermatitis severity scores, TEWL value and blood flow perfusion, and diminished infiltration of inflammatory and mast cells ( p < 0.05 or p < 0.01). In EPM and OFT, EGCG‐treated mice displayed markedly decreased anxiety and depressive‐like behaviors, as manifested by the increased frequency of entries and distance traveled in the open arms and the central area, and elevated 5‐HT levels in serum/brain ( p < 0.05 or p < 0.01). Additionally, compared to the AD model group, EGCG administration reduced MDA content and ROS accumulation in serum/brain/skin, while enhancing GSH‐Px and CAT activity ( p < 0.05 or p < 0.01). Flow cytometry analysis further demonstrated that EGCG could suppress the generation of ROS in TNF‐α/IFN‐γ‐stimulated NHEK cells. Mechanistically, by virtue of in vivo, in vitro and in silico assays, the results of western blot, immunofluorescence and molecular docking discovered that EGCG could promote Nrf2 nuclear translocation and ultimately reduce oxidative stress, at least in part, by specifically targeting Keap1 to disrupt the interaction of Keap1/Nrf2 and modulation of the Keap1/Nrf2/HO‐1 axis. These aforementioned findings highlight the potential of EGCG as a promising therapeutic strategy for AD and its psychiatric comorbidities by alleviating Keap1/Nrf2/HO‐1‐mediated oxidative stress. Administration of EGCG attenuates atopic dermatitis (AD)‐like skin manifestations. EGCG ameliorates AD‐associated behavioral deficits by altering the HPA axis dysfunction and upregulating the neurotransmitters. Down‐regulation of Keap1/Nrf2/HO‐1 signaling pathway‐mediated oxidative stress might be the crucial mechanism for EGCG against AD‐like phenotype accompanied by psychological disorders.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingwang Ren,Guoqing Sun,Xiaoyu Ding,Xiaolin Yu,Li He,Shiji Xiao,Minjian Dong,Jianwen Yang,Kan Ding,Chengxin Sun
Polysaccharides are among the key bioactive components of Gynostemma pentaphyllum (Thunb.) Makino . In this study, a novel polysaccharide fraction, GPPA1-1, was isolated from G. pentaphyllum and purified using DEAE-Sepharose Fast Flow, Sephadex G-75, and Sepharose CL-6B column chromatography. GPPA1-1 was determined to have a molecular weight of 3.7 × 10 4 Da and was composed of mannose (Man), glucuronic acid (GlcA), rhamnose (Rha), glucose (Glc), galactose (Gal), and arabinose (Ara) in a molar ratio of 3.88:3.97:21.77:7.02:45.65:17.71. Comprehensive structural characterization was conducted using Congo red staining, FT-IR spectroscopy, Methylation analysis, and NMR analysis. The backbone of GPPA1-1 was identified as comprising →4)-α-Gal p- (1→, →4)-α-Gal p- (3,1→, →2)-α-Rha p- (4,1→, and →2)-α-Rha p- (1→, with branch chains containing α-Ara f -(1→, →5)-α-Ara f -(1→, →3)-α-Galp-(1→, and -α-GlcA-(1→. Biological assays demonstrated that GPPA1-1 effectively alleviates insulin resistance by inhibiting Galectin-3. This activity was confirmed through various experiments, including Galectin-3-mediated hemagglutination, Western blotting, CETSA, and glucose uptake assays. These findings highlight the potential of GPPA1-1 as a promising therapeutic agent for managing insulin resistance.
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 31
·1-11
·
IF 2.8
Li Shanshan,Zhang Duo,Tian Zhongqing,Wang Fei,Meng Fancheng
Ba[(Zn 0.6 Mg 0.4 ) 1/3 Nb (2- x )/3 Sb x /3 ]O 3 (BZMNS, x = 0.15, 0.2, 0.25, 0.3, 0.35) ceramics were synthesized by the conventional solid-state reaction method. The crystal structure and microwave dielectric properties of the ceramics were investigated. X-ray diffraction (XRD) analysis revealed that the BZMNS ceramics exhibit a cubic perovskite structure. The crystal structures were refined, and no secondary phases were detected in all compositions. The doping of Sb 5+ hinders grain growth, and the average grain size first increases and then decreases with the increase of the x value. The dielectric constant shows a downward trend due to the influence of the ionic bond character of B-site ions and the B-site Rattling effect. The packing fraction is the main factor affecting the Q × f value of BZMNS ceramics. As the x value increases, the temperature coefficient of the resonant frequency ( τ f ) gradually decreases. Ion polarizability dilution is the primary mechanism for regulating τ f of BZMNS ceramics. The BZMNS ceramics sintered at 1480 °C with x = 0.3 exhibit optimal microwave dielectric properties ( ε r = 31.44, Q × f = 62,273 GHz (at f₀ = 6.5 GHz), τ f = + 2.48 ppm·°C −1 ), indicating that this ceramic has good application prospects.
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Toxics
·
2025
·Vol. 13
·期 4
·274
·
IF 4.1
Jie Gu,Huilin Jin,Jun Hu,Jian Wang,Daqiang Yin
Endocrine-disrupting chemicals (EDCs) are exogenous substances discharged into the environment through human activities. 3-Isopropylphenol, a typical alkylphenol-based EDC, has been extensively studied due to its broad application and potential ecological impacts. However, the mechanism of its neurotoxicity remains unclear. In this study, the neurotoxic effects of 3-isopropylphenol were examined using the zebrafish model. We predicted its potential toxic mechanisms and action targets using network toxicology and molecular docking and verified them via RT-qPCR. Results showed that 3-isopropylphenol exposure inhibits the cAMP/PKA signaling pathway in zebrafish larvae, promoting apoptosis, impairing neural development, and suppressing locomotor behavior. These findings enhance our understanding of the toxic effects and mechanisms of 3-isopropylphenol on zebrafish larval neural development and aid in evaluating its potential ecological hazards.
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Nutrition & Food Science
·
2025
·
IF 1.6
Ye Yue,Huasong Bai,Tong Liu,Shang Jia,Xiaohong Kou,Zhanzhong Wang
Purpose This study aims to investigate the impact of the compositions and concentrations of α -linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are significant n-3 polyunsaturated fatty acids (n-3 PUFAs), on the anti-inflammatory properties of lipopolysaccharide (LPS)-stimulated mouse hepatocytes. Design/methodology/approach A mouse hepatocyte inflammation model was established using LPS to evaluate the anti-inflammatory effects of a combination therapy with EPA, ALA and DHA. Interleukin-10 (IL-10), lipoxin A4 (LXA4) and tumor necrosis factor-α(TNF- α ) were measured to evaluate the anti-inflammatory ability of hepatocytes. The compositions and concentrations of EPA, ALA and DHA were optimized by response surface methodology to obtain the best anti-inflammatory effect. Findings The optimal inflammatory model was established with 10 µg/mL LPS treatment for 24 h. Maximal cell viability was found at EPA concentration of 150 µM, ALA concentration of 400 µM and DHA concentration of 600 µM. The optimal concentrations of n-3 PUFAs for anti-inflammatory activity in mouse hepatocytes were obtained through single-factor experiments and response surface analysis, with EPA 141.41 µM, ALA 405.45 µM and DHA 551.76 µM. Under optimized conditions, the experimental results for TNF- α , IL-10 and LXA4 levels were close to the theoretical prediction, indicating that the mathematical model was extremely accurate and versatile. Originality/value This study systematically explored the specific effects of different ratios and concentrations of ALA, EPA and DHA on the anti-inflammatory ability of mouse hepatocytes, offering an experimental foundation for the advancement of novel anti-inflammatory medicines and nutritional supplements.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2020
·
IF 4.872
Jinglou Chen,Jianhua Liu,Yongfang Lei,Min Liu
The environmental pollution caused by cigarette smoke (CS) seriously endangers people's health. Epigallocatechin-3-gallate (EGCG) is the most abundant catechin in green tea. In this study, rats were exposed to CS for 90 days. Kidney function was evaluated by detecting the levels of serum creatinine and blood urea nitrogen. Liver function was evaluated by detecting the activities of alanine aminotransferase and aspartate transaminase. The renal and hepatic oxidative stress and inflammation were assessed by detecting the levels of malondialdehyde, reduced glutathione , antioxidant enzymes (superoxide dismutase and glutathione peroxidase) and proinflammatory cytokines. Organ fibrosis was evaluated by observing collagen deposition via masson staining, by examining the hydroxyproline level, by measuring the mRNA levels of fibrosis-associated genes collagen (Col)-1A1 and Col-3A1, as well as by assessing the activity of profibrotic TGF-β1 pathway. Additionally, renal and hepatic epithelial-mesenchymal transition (EMT) were evaluated. It was observed that EGCG ameliorated the renal and hepatic oxidative stress, inflammation, EMT, as well as inhibited the activation of TGF-β1 signaling pathway induced by CS. These results showed that EGCG could attenuate CS-induced renal and hepatic fibrosis.
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