Pharmaceutics
·
2019
·Vol. 11
·期 3
·107
·
IF 4.773
Huiyun Zhang,Qilong Wang,Congyong Sun,Yuan Zhu,Qiuxuan Yang,Qiuyu Wei,Jiaxin Chen,Wenwen Deng,Michael Adu-Frimpong,Jiangnan Yu,Ximing Xu
6-shogaol is a promising anti-cancer and anti-inflammatory agent. However, the treatment effectiveness of 6-shogaol is limited by poor water solubility, poor oral absorption and rapid metabolism. Herein, 6-shogaol loaded in micelles (SMs) were designed to improve 6-shogaol’s solubility and bioavailability. The micelles of a PEG derivative of linoleic acid (mPEG2k-LA) were prepared by the nanoprecipitation method with a particle size of 76.8 nm, and entrapment of 81.6 %. Intriguingly, SMs showed a slower release in phosphate buffer saline (PBS) (pH = 7.4) compared to free 6-shogaol while its oral bioavailability increased by 3.2–fold in vivo. More importantly, the in vitro cytotoxic effect in HepG2 cells of SMs was significantly higher than free 6-shogaol. Furthermore, SMs could significantly improve the tissue distribution of 6-shogaol, especially liver and brain. Finally, SMs showed a better hepatoprotective effect against carbon tetrachloride (CCl4)-induced hepatic injury in vivo than free 6-shogaol. These results suggest that the novel micelles could potentiate the activities of 6-shogaol in cancer treatment and hepatoprotection.Keywords:6-shogaol;cytotoxic effect;HepG2 cells;micelles;hepatoprotection
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomed Research International
·
2018
·
IF 2.583
Juan Huang,Yun Yang,Yaokun Wang,Mingyang Zhang,Youxun Liu
The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2’-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. Malachite green solution was detoxified completely after biodegradation by Im-LMS. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. The activity of Im-LMS was almost completely stable after being stored for 10 days. The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2017
·
IF 4.44
Dandan Liu,Jinyu Li,Bingchao Cheng,Qingyin Wu,Hao Pan
This study is focused on further understanding the characteristics of chitosan-N-acetylcysteine surface-modified nanostructured lipid carriers (CS-NAC-NLCs) in their interaction with ocular mucosa. Coumarin-6 (C6)-labeled NLCs, including uncoated NLCs, chitosan hydrochloride (CH)-, and CS-NAC-coated NLCs, were developed using a melt-emulsification technique and subsequently decorated with different types or portions of chitosan derivatives. Mucoadhesion was evaluated ex vivo using a flow-through process with fluorescence detection. The results demonstrated that the presence of CS-NAC on the C6-NLC surface provided the most obvious enhancement in adhesion due to the formation of both noncovalent (ionic) and covalent (disulfide bridges) interactions with mucus chains. Meanwhile, the concentration of CS-NAC in the formulation positively influenced the viscosity of the nanoparticles and hence prolonged their retention in the ocular tissue. Transcorneal penetration studies revealed that CS-NAC-NLC particles were able to penetrate through the entire corneal epithelium primarily via a transcellular route. The transport depth and velocity strongly relied on the modification material and the particle size. Ex vivo fluorescence imaging and in vivo ocular distribution investigations showed that C6 was broadly distributed in rabbit eye tissues and absorbed by aqueous humor after CS-NAC-NLC instillation. In relation to C6 eye drops, CS-NAC-NLCs achieved considerably higher Cmax (4.01-fold), MRT0-∞ (1.87-fold), and AUC0-∞ (16.29-fold) in the aqueous humor. Moreover, the increase in drug absorption was greater in the cornea than in the conjunctiva. Thereby, it is possible to draw a conclusion that CS-NAC-NLCs presented great potential for drug application to the front portion of the eye.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 9
·2068
·
IF 4.2
Kai Cai,Qiang Liu,Yechun Lin,Xingyou Yang,Qi Liu,Wenjie Pan,Weichang Gao
2, 6-diisopropylaniline (2, 6-DIPA) is a crucial non-intentionally organic additive that allows the assessment of the production processes, formulation qualities, and performance variations in biodegradable mulching film. Moreover, its release into the environment may have certain effects on human health. Hence, this study developed simultaneous heating hydrolysis–extraction and amine switchable hydrophilic solvent vortex-assisted homogeneous liquid–liquid microextraction for the gas chromatography–mass spectrometry analysis of the 2, 6-DIPA additive and its corresponding isocyanates in poly(butylene adipate-co-terephthalate) (PBAT) biodegradable agricultural mulching films. The heating hydrolysis–extraction conditions and factors influencing the efficiency of homogeneous liquid–liquid microextraction, such as the type and volume of amine, homogeneous-phase and phase separation transition pH, and extraction time were investigated and optimized. The optimum heating hydrolysis–extraction conditions were found to be a H2SO4concentration of 2.5 M, heating temperature of 87.8 °C, and hydrolysis–extraction time of 3.0 h. As a switchable hydrophilic solvent, dipropylamine does not require a dispersant. Vortex assistance is helpful to speed up the extraction. Under the optimum experimental conditions, this method exhibits a better linearity (0.0144~7.200 μg mL−1withR= 0.9986), low limit of detection and quantification (0.0033 μg g−1and 0.0103 μg g−1), high extraction recovery (92.5~105.4%), desirable intra- and inter-day precision (relative standard deviation less than 4.1% and 4.7%), and high enrichment factor (90.9). Finally, this method was successfully applied to detect the content of the additive 2, 6-DIPA in PBAT biodegradable agricultural mulching films, thus facilitating production process monitoring or safety assessments.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2024
·
IF 3.5
Han-Yu Liu,Pan Ning,Feng Qian,Yao-Wu Wang,Hai-Min Zhang,Pu Wang
Flavin-dependent halogenase (FDH) is highly prized in pharmaceutical and chemical industries for its exceptional capacity to produce halogenated aromatic compounds with precise regioselectivity. This study has devised a multi-enzyme self-assembly strategy to construct an effective and reliable in vitro coenzyme cycling system tailored for FDHs. Initially, tri-enzyme self-assembling nanoclusters (TESNCs) were developed, comprising glucose dehydrogenase (GDH), flavin reductase (FR) and FDH. The TESNCs exhibited enhanced thermal stability and conversion efficiency compared to free triple enzyme mixtures during the conversion of L -Trp to 6-Cl- L -Trp, resulting in a 2.1-fold increase in yield. Subsequently, an ordered co-immobilization of GDH, FR, and FDH was established, further amplifying the stability and catalytic efficiency of the FDH coenzyme cycle system. Compared to the free TESNCs, the immobilized TESNCs demonstrated a 4.2-fold increase in catalytic efficiency in a 5 mL reaction system. This research provides an effective strategy for developing a robust and efficient coenzyme recycling system for FDHs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
·
2025
·
IF 1.9
Sarmad Soomro,Zhenkun Sun,Dennis Y. Lu,Zhihao Zhou,Lin Li,Lunbo Duan
Catalyst deactivation due to carbon deposition and sintering poses a significant challenge to the efficient dry reforming of CH 4 and CO 2 into syngas, particularly at high Ni loadings. To tackle this difficulty, ordered mesoporous silicas with varied pore symmetries were employed as supports in this study to synthesize the Ni-based catalysts using hydrothermal treatment. The 10% Ni/MCM-41 catalyst exhibited the highest conversion rates for CO 2 (81%) and CH 4 (77%), with favourable H 2 /CO (0.92) ratios indicating efficient syngas production Higher Ni concentrations increase active sites and catalytic performance, they also increase carbon deposition, reducing catalyst durability. At lower Ni loadings (4% Ni), catalysts with a 3D pore structure showed improved activity and stability, effectively reducing carbon deposition. This study demonstrates that optimized mesopore symmetries and Ni loadings can enhance catalyst efficiency and durability for syngas production.
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hui Pan,Meng Jin,Fang Zhang,Xuelei Chen,Mengping Zhang,Haiyan Wei,Xiao Meng,Wenwen Chen,Zhihu Zhang,Cuijuan Wang
Health concerns over mixed pesticide residues highlight the need for rapid detection methods. This study developed a multifunctional point-of-use (POU) sensor that integrates separation, enrichment, and surface-enhanced Raman spectroscopy (SERS) using high-index Au trisoctahedrons (TOHs) and metal-organic frameworks (MOFs) on polyamide 6 (PA-6) film. The Au TOHs with sharp tips and high index facets offer excellent SERS performance, while MOFs facilitate analyte enrichment and separation. The flexible PA-6 film was used as an absorbent for the initial adsorption. Using the AuTOHs@ZIF-67/PA-6, we achieved on-site detection of chlorfenapyr (CFP) and emamectin benzoate (EB) in 5 min without pre-treatment, significantly faster than traditional methods. Detection limits were 0.68 ppb for CFP and 0.26 ppb for EB on cucumber surfaces, and 0.13 ppb and 0.95 ppb, respectively, in cucumber juice, well below tolerance levels. This method shows great promise for detecting pesticide mixtures in non-laboratory settings, offering a new POU alternative.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER COMPOSITES
·
2024
·
IF 4.8
Xinyi Jing,Linggong Meng,Tong Liu,Xinming Ye,Shuai Yang,Zhichao He,Wensheng Wang,Jie Li,Yingchun Li
Achieving high thermal conductivity in composites with low filler content is a major challenge in current research. In this paper, polypropylene (PP) and polyamide 6 (PA6) were used as polymer matrix, and the hybrid thermal conductive filler, formed by hexagonal boron nitride (h-BN) and aminated carbon nanotubes (NH 2 -CNTs), was integrated into matrix for the fabrication of thermal conductive composites (PP/PA6/h-BN and PP/PA6/h-BN@CNTs) via two-step melt blending technology. The thermal conductive fillers were selectively distributed in a phase of the polymer matrix with a co-continuous structure, forming a thermal conductive path with a three-dimensional network structure. The results show that when the content of h-BN reaches 25 wt%, the thermal conductivity of the PP/PA6/h-BN and PP/PA6/h-BN@CNTs composites was 199% and 300% higher than that of PP/PA6, respectively. Through testing the volume resistivity of the composites, it was confirmed that PP/PA6/h-BN@CNTs composites maintain good electrical insulation. Both composites demonstrated remarkable thermal conductivity and outstanding electrical insulation. This research provides a facile way to prepare highly thermal conductivity polymer composites with excellent processing performances and electrical insulation. Highlights h-BN@CNTs hybrid fillers with sandwich structure were prepared. A two-step blending method was used to control the selective distribution of hybrid fillers in a phase of the polymer matrix with a co-continuous structure. The hybrid filler formed a thermal conductive path with a three-dimensional network structure in the collective. PP/PA6/h-BN@CNTs composites demonstrated remarkable thermal conductivity, outstanding electrical insulation and favorable processing performances.
✓ 已验证
DOI
高影响因子
JOURNAL OF HYPERTENSION
·
2018
·Vol. 36
·期 4
·834-846
·
IF 4.099
Yang Shuai,Chen Dandan,Chen Fan,Zhao Xinmei,Zhang Yubin,Li Zhe,Jin Liang,Xu Yitao,Sanchis Daniel,Ye Junmei
Objective:
Protein kinase B2 (AKT2) is implicated in cardiomyocyte survival during various stress conditions. However, the role of AKT2 in heart function, cardiac hypertrophy and blood pressure (BP) control during hypertension is not fully understood. Therefore, we sought
to determine whether the deletion of AKT2 protects cardiac function during angiotensin II/high-salt-diet (AngII/HSD) treatment and find out the signaling pathway.Methods:
Male C57BL/6J (wild type), AKT2 knockout and interleukin (IL)-6 knockout
mice were fed a 4% NaCl diet for 5 weeks. In the last week, mice were split in two groups and infused subcutaneously with either vehicle or AngII (1.5 μg/h per mouse) for 1 week. Then, BP and cardiac function were assessed. Immunohistology of IL-6 and monocyte chemoattractant protein
1 was performed to detect inflammation in the heart. Masson's trichrome staining was performed to evaluate cardiac fibrosis. Heart tissue homogenates and neonatal mice cardiomyocytes were collected to analyze oxidative stress.Results:
Compared
with wild-type mice, AKT2 knockout mice maintained BP and showed better left ventricle ejection fraction, lower level of fibrosis, reduced oxidative stress, reduced IL-6 expression and less macrophage infiltration, when treated with AngII/HSD. IL-6 knockout mice treated with AngII/HSD also
showed alleviated left ventricular function, fibrosis, oxidative stress and macrophage infiltration compared with wild type.Conclusion:
AKT2 deficiency prevents the development of AngII/HSD-induced hypertension, cardiac dysfunction
and myocardial injury including oxidative stress, fibrosis and inflammation by suppressing IL-6 expression. These data reveal an important role of the AKT2-IL-6 pathway in mediating AngII/HSD-induced hypertension and cardiomyopathy.
✓ 已验证
DOI
PubMed
高影响因子
ADSORPTION SCIENCE & TECHNOLOGY
·
2013
·
IF 0.559
Ping Yu,Qilong Sun,Jianming Pan,Zhenjiang Tan,Jiangdong Dai,Yongsheng Yan,Feng Cheng
Poly(styrene–divinylbenzene)/Fe3O4 (Fe3O4@St–DVB) magnetic porous polymer microspheres based on suspension polymerization were prepared for adsorption of 2, 4-dichlorophenol (2, 4-DCP) and 2, 6-dichlorophenol (2, 6-DCP) from aqueous solution. The as-prepared product was characterized by Fourier transform infrared spectroscopy, Raman, X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, differential thermal analysis and elemental analysis. The results showed that the composite material has both porous and magnetic characteristics, which aid in faster separation and increase in adsorption capacity. The adsorption performance of the Fe3O4@(St–DVB) magnetic porous microspheres was manifested by batch mode adsorption experiments with respect to pH, initial concentration, contact time and temperature. The adsorbent was found to be sensitive to changes in pH, and the adsorption capacity of 2, 4-DCP is higher than that of 2, 6-DCP. The kinetics experimental data fitted well with the pseudo-second-order model, as three steps belonged to the pseudo-second-order adsorption process. The adsorption isotherms were also described by the Langmuir and Freundlich isotherm models, respectively. It was found that the Langmuir isotherm model fitted the equilibrium data superior to the Freundlich model. Thermodynamic parameters were calculated by the Gibbs free energy function, confirming that adsorption process was spontaneous and endothermic. In addition, the reusability performance of the Fe3O4@(St–DVB) magnetic porous microspheres was demonstrated by four repeated cycles.
✓ 已验证
DOI
高影响因子
Polymers
·
2020
·Vol. 12
·期 5
·1132
·
IF 3.426
Bo Tao,Zongning Yin
Dopamine-modified hyaluronic acid (HA-DOP) was chosen as the drug carrier in this study, and Cu2+was selected from among Cu2+, Zn2+, Fe2+, and Ca2+as the central atom. 6-Mercaptopurine (6-MP) was conjugated with HA through a coordination reaction. HA-DOP-copper-MP (HA-DOP-Cu-MP), a redox-responsive coordination polymer prodrug, was prepared. The drug loading was 49.5 mg/g, the encapsulation efficiency was 70.18%, and the particle size was 173.5 nm. HA-DOP-Cu-MP released rapidly in the release medium containing reduced glutathione (GSH), and the accumulated release exceeded 94% in 2 h. In the release medium without GSH, the drug release rate was slow, with only 15% of the 6-MP released in 24 h. Cell uptake experiments revealed the CD44 targeting of HA. Cell viability assays showed that the cytotoxicity of HA-DOP-Cu-MP was higher than that of free 6-MP. Indeed, HA-DOP-Cu-MP is very toxic to cancer cells. In this paper, the redox-responsive drug delivery system was synthesized by a coordination reaction. The tumour targeting and tumour cytotoxicity of 6-MP were improved.Keywords:coordination;hyaluronic acid;6-Mercaptopurine;redox-responsive;targeting
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Neuroinflammation
·
2025
·Vol. 22
·期 1
·1-19
·
IF 10.1
Chi Zhexi,Lu Bo,Liu Rongjun,Pan Chen,Meng Bo,Xing Xiuzhong,Yuan Hui,Wu Xuewei,Chen Yushan,Ren Yuxuan,Wu Wenwei,Miao Mengmeng,Chen Junping,Chen Xiaowei
Neuropathic pain, a debilitating nerve injury-induced condition, remains a significant clinical challenge. This study evaluates the effect of histone deacetylase 6 (HDAC6) inhibition in a spared nerve injury (SNI) mouse model. Systemic administration of the selective HDAC6 inhibitor ACY-1215 (20 mg/kg/day, 14 days), alleviated SNI-induced pain in mice of both sexes. ACY-1215 increased acetylated signal transducer and activator of transcription 3 (Ac-STAT3) and reduced phosphorylated STAT3 (p-STAT3) in the lumbar spinal cord of SNI mice. HDAC6 and p-STAT3 were expressed in spinal dorsal horn neurons, and SNI-enhanced HDAC6/STAT3 interaction was reversed by ACY-1215. Neuronal STAT3 overexpression induced pain hypersensitivity and elevated p-STAT3, tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), effects suppressed by ACY-1215. Cytokine profiling identified CC-chemokine ligand 7 (CCL7) as a key downstream effector of the HDAC6/STAT3 axis, with ACY-1215 attenuating SNI-induced CCL7 upregulation. HDAC6 knockdown in neurons reduced p-STAT3, while HDAC6 or STAT3 knockdown diminished CCL7 production. These findings demonstrate that ACY-1215 mitigates neuropathic pain by modulating STAT3 acetylation/phosphorylation and suppressing HDAC6/STAT3-driven CCL7 and cytokine release. This study underscores the role of the HDAC6/STAT3/CCL7 signaling axis in neuropathic pain and highlights the therapeutic potential of HDAC6 inhibitors for pain management.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）