FOOD RESEARCH INTERNATIONAL
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2026
·
IF 8.8
Hong Li,Haiying Cui,Tariq Aziz,Ashwag Shami,Abdelrahman R. Ahmed,Fakhria A. Al-Joufi,Lin Lin
Inhibitory mechanism of phloretin on the AgrA LytTR- agr complex was revealed. • The AgrA LytTR domain protein was purified successfully. • EMSA confirmed phloretin may disrupt the binding of AgrA LytTR domain and P3 promoter. • MD simulations revealed that phloretin sterically hindered AgrA- agr binding, validated by mixed fluorescence quenching. • Phloretin acts as QSI to inhibit the secretion of enterotoxin and hemolysin in beef. Staphylococcus aureus ( S. aureus ) represents a major foodborne pathogen whose enterotoxin production poses significant challenges to food safety due to its high environmental resistance and limited efficacy of conventional sterilization. Since the expression of enterotoxins is predominantly governed by the agr quorum sensing system, targeting this regulatory pathway has become a strategic choice for virulence control. This study elucidated the mechanism by which phloretin, a potential quorum sensing inhibitor, interferes with the agr system to attenuate virulence. To achieve this, the recombinant AgrA LytTR domain was expressed and purified, and its interaction with phloretin was characterized using thermal shift assays (TSA), electrophoretic mobility shift assays (EMSA), and molecular dynamics (MD) simulations. The results showed that phloretin specifically binds to the AgrA LytTR domain, enhancing its thermal stability and disrupting AgrA LytTR- agr operon binding by reducing the free energy of interaction between them, without causing significant structural rearrangement. Mechanistic analysis indicated that phloretin sterically hinders key β -sheet turn residues (HIS169, ASN201, ARG233), thereby impairing DNA recognition, downregulating RNAIII transcription, and inhibiting agr signaling. In cooked beef, phloretin significantly inhibited the secretion of enterotoxins and α -hemolysin, while delaying lipid oxidation and protein degradation, and maintaining the meat texture. These findings suggested that phloretin is a multifunctional substance with anti-virulence, antioxidant, and preservative properties, demonstrating its potential as a natural food preservative. Download: Download high-res image (118KB) Download: Download full-size image
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BIORESOURCE TECHNOLOGY
·
2026
·
IF 8.2
Hangbin Lei,Xuefu Zeng,An-Ping Zeng,Shizhen Wang
Conductive DES stabilizes hydrogenase conformation via a dynamic hydrogen-bond network. • DES-modified MOF creates an anaerobic microenvironment that enhances the oxygen tolerance of the immobilized hydrogenase. • DES induces a 3D nanoflower to 2D nanosheet morphological transformation of the immobilized hydrogenase. Hydrogenases can reversibly catalyze the conversion of protons and electrons into hydrogen, which are widely applied in green biohydrogen production. However, hydrogenases face challenges of poor stability and oxygen sensitivity. In this work, conductive deep eutectic solvents (DES) modified metal–organic frameworks (MOFs) were studied as carriers for Pyrococcus furiosus [NiFe]-hydrogenase (pfSHI) immobilization to improve the stability and oxygen tolerance of the enzyme. Choline chloride-ethylene glycol (ChCl-EG) modification of MOFs stabilized the enzyme conformation. After 8 reuses, pfSHI@ChCl-EG-ZIF-67 retained 63.02 % relative activity, higher than that of pfSHI@ZIF-67 (56.18 %). This enhancement is due to ChCl-EG with dynamic hydrogen-bond network. Furthermore, ChCl-EG regulated the pore of MOFs through non-covalent host–guest interactions to create the anaerobic microenvironment, which enhances oxygen tolerance of hydrogenase. After 48 h of air exposure, pfSHI@ChCl-EG-ZIF-67 retained 45.64 % activity, representing 2.67-fold increases compared to free pfSHI. Additionally, ChCl-EG in-situ modulated the structural evolution from 3D nanoflowers (pfSHI@ZIF-67) to 2D nanosheets (pfSHI@ChCl-EG-ZIF-67) during hydrogenase immobilization. The k cat / K m of pfSHI@ChCl-EG-ZIF-67 was 851 s −1 ·mM −1 , which was 1.49-fold of that of pfSHI@ZIF-67. The immobilized hydrogenases by DES-modified MOF exhibits enhanced stability and oxygen tolerance, which is promising for applications in biohydrogen production. Download: Download high-res image (158KB) Download: Download full-size image
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Xie Xiaoling,Xiang Zhenyang,Yang Ruhui,Wu Xuanlu,Guo Jinyu,Fang Ruolin,Zan Xingjie,Huang Wenjuan,Tong Yuhua
Corneal neovascularization (CNV) is one of the leading causes of corneal blindness, affecting millions of people worldwide. Anti-vascular endothelial growth factor agents, such as Bevacizumab (Beva), offer high specificity and low side effects. However, their limited ability to penetrate the corneal barrier necessitates invasive administration, significantly restricting their clinical application. Herein, we engineered (Beva&C₂G₂R₉)@Zn nanoparticles formed by co-assembling Beva, C₂G₂R₉ peptide and Zn 2+ , which decrease in size over time, as an efficient strategy for noninvasive Beva delivery across the corneal barrier to treat CNV. By combining various technologies (DLS, TEM, XPS, FTIR, and computer simulation), we discovered that the coordination between Beva and Zn 2+ drives the nanoparticle formation, while the C₂G₂R₉ peptide facilitates its size evolution. Compared to size-stable nanoparticles of Beva@Zn and (Beva&R₉)@Zn, (Beva&C₂G₂R₉)@Zn nanoparticles exhibit rapid cellular internalization, efficient lysosomal escape, and effective corneal barrier penetration, leading to efficiently inhibit HUVEC cell migration and tube formation. Importantly, in a rat alkali-burned CNV model, (Beva&C₂G₂R₉)@Zn nanoparticles exhibited superior efficacy in inhibiting corneal neovascularization compared to size-stable nanoparticles, with the lowest inflammation index. The results of this study highlight the importance of controlling the size of nanoparticles to enable non-invasive delivery of macromolecular drugs across the corneal biological barrier, offering new insights for the design of future nanoparticle-based drug delivery systems. Graphical
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Frontiers in Nutrition
·
2026
·
IF 5.5
Bingye Xu,Xiaoxiao Li,Rong Liu,Ying Han,Pan Zhen,Hanyue Fu,Huan Lv,Fan Wei,Haoxin Cui,Bowei Zhang,Shuo Wang
IntroductionHyperuricemia (HUA) is a major risk factor for gout and renal injury, underscoring the need for safe and effective natural dietary interventions. This study investigated the chemical composition and antihyperuricemic effects of dealcoholized Zhuyeqing liquor extract (ZLE) using both cellular and animal models.MethodsThe nutritional composition and phytochemical profile of ZLE were characterized by quantitative analysis and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The antihyperuricemic activity of ZLE was evaluated in adenosine-induced HepG2 cells and in a hypoxanthine/potassium oxonate-induced mouse model of HUA by assessing uric acid (UA) metabolism, hepatorenal function, urate transporter expression, and renal inflammatory signaling.ResultsQuantitative analysis showed that ZLE is rich in polysaccharides, terpenes, and flavonoids. UPLC–MS/MS identified 389 phytochemical constituents, predominantly iridoid glycosides, organic acids, and flavonoids. In adenosine-induced HepG2 cells, ZLE significantly reduced UA production and xanthine oxidase (XOD) activity. In HUA mice, ZLE dose-dependently decreased serum UA levels, improved renal and hepatic function by reducing serum creatinine, blood urea nitrogen, alanine aminotransferase, and aspartate aminotransferase levels, and inhibited hepatic XOD activity. Furthermore, ZLE restored renal urate homeostasis through coordinated regulation of key urate transporters and alleviated HUA-induced renal inflammation and histopathological injury by suppressing the lipopolysaccharide-mediated NLRP3/Caspase-1 signaling pathway.DiscussionThese findings demonstrate that ZLE exerts antihyperuricemic and renoprotective effects through coordinated modulation of uric acid production, renal urate transport, and inflammatory signaling. The results support the potential application of ZLE as a functional food ingredient for dietary management of HUA and provide a scientific basis for translating traditional dietary resources into modern nutritional interventions.
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Science Immunology
·
2025
·Vol. 10
·期 108
·
IF 16.3
Limin Cao,Li Wang,Zhihong Li,Xia Wei,Jinqiu Ding,Chun Zhou,Xia Chen,Zhicheng Huang,Zhugui Shao,Junchen Shen,Hongfei Lou,Keqing Zhao,Yuwei Huang,Yuanqin Yang,Han Liu,Yumeng Sun,Junling Niu,Shan Jiang,Rong Lu,Longhai Tang,Xiaoming Zhang,Haibing Zhang,Yichuan Xiao,Jianfeng Chen,Shixin Ma,Chengjiang Gao,Guangxun Meng,Li Liu,Zhaozhu Qiu,Haopeng Wang,Liufu Deng,Youqiong Ye,Xin-Ming Jia,Huabin Li,Hui Xiao
The volume-regulated anion channels (VRACs) transport osmolytes, neurotransmitters, and cyclic GMP-AMP (cGAMP) across the cell membrane to regulate cell volume and host defense. We report that the leucine-rich repeat–containing 8A/C (LRRC8A/C) VRAC plays a crucial role in immune responses to radiotherapy and chemotherapy for cancer. VRACs transfer cGAMP from irradiated cancer cells to infiltrating CD4 and CD8 T cells, thus enhancing their effector functions. TCR signaling acts as a physiological signal to open the VRAC pore through phosphatidylinositol 4,5-bisphosphate [PI(4,5)P 2 ] and reactive oxygen species (ROS). This allows the rapid uptake of cGAMP and STING activation in mouse and human T cells and induction of interferon-α/β, which up-regulate granzymes and IFN-γ in CD8 T cells. Inhibition of the extracellular hydroxylases CD39 and ENPP1 maintains extracellular ATP and cGAMP, which promotes VRAC-enhanced CD8 T cell anticancer function. Thus, the transfer of cGAMP to T cells by VRACs may be a strategy that can be targeted in future cancer therapies.
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Horticulturae
·
2025
·Vol. 11
·期 11
·1292
·
IF 3
Hu Zhao,Xiaopu Shi,Zilu Zhao,Juan Wang,Baul Ko,Jong Hyang Bae,Yang Gyu Ku,Ho Cheol Kim,Chul Min Kim
Young leaves and sprouts ofToona sinensisare widely consumed throughout Asia. However, they are highly perishable, resulting in a short shelf life and limited marketability. Low-temperature storage can extend shelf life but is energy-intensive, restricting large-scale use. Salicylic acid (SA) exhibits preservative properties, representing a promising alternative. In this study, we investigated the effects of 250 μmol SA treatment on postharvestT. sinensisbuds and leaves stored at 20 °C for 7 days and compared it to low-temperature storage (4 °C). SA treatment delayed wilting, reduced water loss, suppressed total soluble solid accumulation, and inhibited active oxygen free radical and malondialdehyde production compared to the untreated controls at room temperature while outperforming low-temperature storage. The treated buds also maintained higher antioxidant enzyme activity and preserved non-enzymatic antioxidant compounds, including ascorbic acid, polyphenols, and flavonoids. Although total amino acid content steadily increased under SA treatment, individual amino acids greatly fluctuated. SA treatment reduced nitrate reductase and glutamate dehydrogenase activities, as well as NO2•−levels, indicating its effects on nitrogen metabolism. Our findings indicate that SA improves the postharvest quality ofT. sinensisbuds during room-temperature storage, providing a theoretical basis for applying SA as a storage agent forT. sinensisbuds.
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JOURNAL OF MOLECULAR STRUCTURE
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2025
·
IF 4.7
Hu Wang,Pengxiao Guo,Chaochuang Yin,Yadong Zhou,Yizhu Lei,Tingting Wu,Yinyan Wang,Kang Chen,Li Sun,Ziao Zong,Huaijun Luo
This study develops four copper(II)-based coordination complexes ( C1 - C4 ) as potential urease inhibitors through rational ligand design. By combining fluorinated Schiff base ligands with modified bipyridine auxiliary ligands, we systematically investigated how electronic and steric factors influence urease enzyme inhibition. The complexes were synthesized and comprehensively characterized including crystallographic analysis, In each of the four complexes, the central Cu(II) exhibits a five-coordinate geometry, incorporating ONO atoms from the Schiff base ligand and two nitrogen atoms from the auxiliary ligand, which results in a distorted square pyramidal configuration. The biological evaluation identified the complex C1 as exhibiting significantly improved urease inhibitory activity, with an IC 50 value of 8.88 ± 1.15 μM, in comparison to the standard reference acetohydroxamic acid, which has an IC 50 value of 27.73 ± 2.93 μM. Computational approaches complemented experimental studies to decipher the molecular basis of activity differences among the complexes. On this basis, the structural activity relationship study shows that the urease inhibitory activity of complexes is influenced by electron effect and steric hindrance, providing essential insights for developing new urease inhibitors and advancing their biomedical applications.
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JOURNAL OF NANOBIOTECHNOLOGY
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2025
·Vol. 23
·期 1
·1-23
·
IF 12.6
Wu Lihuang,Wang Yuqi,Zhao Xinyue,Zhao Tingting,Li Junhua,Kuang Yi,He Yiyan,Yang Shengxiang,Gu Zhongwei,Mao Hongli
Full-thickness cutaneous wounds pose a significant threat to global health due to their complex healing demands. Standard clinical wound dressings often fall short in providing the adaptability and functionality required for the entire healing process. While hierarchically engineered nanofiber dressings have shown advancement in wound management, challenges such as material compatibility and interfacial bonding during their design have limited both manufacturing and therapeutic outcomes. This study introduces a self-adhesive hierarchical nanofiber (SAHN) patch designed to provide a comprehensive and dynamic approach to wound care. The SAHN patch strategically integrates synthetic biodegradable poly(ester carbonate) with natural bioactive components, forming a seamless dual-layer system that offers both immediate protection and sustained bioactivity to support tissue regeneration. In vitro and in vivo studies demonstrate the patch’s superior interlayer adhesion, soft tissue adhesion, controlled degradation, and robust antibacterial capabilities. These features collectively safeguard the wound microenvironment, facilitate hemostasis, manage inflammation, and accelerate wound closure. Our findings highlight the transformative potential of the SAHN patch in improving traditional wound care, overcoming the manufacturing challenges associated with hierarchical nanofiber dressings, and offering a promising solution for dynamic and multistage management of full-thickness cutaneous wounds that aligns with the natural progression of tissue repair. Graphical
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6.1
Baizong Fang,Wei Guo,Zhenyu Liu,Huakui Huang,Xiaohong Zhong
Background The detection and imaging of intracellular biomarkers can provide important information for the early diagnosis of cancer. Leveraging the inherent advantages of DNA such as biocompatibility, ease of synthesis, programmability, and sequence-specific recognition, DNA-based probes have been widely employed for biomarker analysis. Currently, a variety of DNA probes combined with signal amplification methods have been widely used to visualize biomarkers in living cells. However, conventional single-locked DNA probes cannot ensured the accurate diagnosis. Furthermore, the lack of efficient cascade signal amplification strategies makes it challenging to detect and image low-abundance biomarkers. Results In this work, we report a dual-locked, self-feedback catalytic hairpin assembly (CHA)-DNAzyme cascade amplification platform, termed hMNS@SCD. This platform utilizes MnO 2 nanoflowers as nucleic acid carriers and DNAzyme cofactor supplement, employing apurinic/apyrimidinic endonuclease 1 (APE1) and miR-21 as two molecular keys. When the APE1 and miR-21 existence, hairpin probe carry out the CHA cycle, leading to the assembly of a Y-shaped DNAzyme complex. When Mn 2+ is present, the Y-shaped DNAzyme can undergo a hybridization-cleavage-release cycle on the MB, leading to a gradual restoration of Cy5 fluorescence. In addition, the released target analogue from MB can trigger a new CHA cycle, generating more Y-shaped DNAzyme complexs and cleaving more MB, further amplifying the fluorescence signal. Through the self-feedback CHA-DNAzyme cascade amplification mechanism, hMNS@SCD effectively enhances the detection sensitivity, with a detection limit for miR-21 reaching 34.6 fM. Experimental data show that hMNS@SCD has high sensitivity, selectivity, and biocompatibility. Significance we constructed a dual-locked, self-feedback CHA-DNAzyme cascade nanomachine, which enables tumor-specific molecular imaging and effectively distinguishes between normal cells and cancer cells. In addition, due to the programmability of nucleic acid probes, this strategy can be readily adapted for detection and imaging of other low-abundance biomarkers, offering a promising way forward for tumor-specific molecular imaging and early cancer diagnosis.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2026
·
IF 2.2
Ya Li,Xinxin Li,Huaxian Li,Weiwei Yan,Qing Dai,Peijun Han,Wenlan Wang
This study aimed to investigate the ameliorative effect of hyperbaric oxygen (HBO) on a rat model of hyperuricemia induced by combined drugs and its underlying mechanism. A rat model of drug-induced hyperuricemia (HUA)was established by using a combination of yeast, potassium oxonate (PO) and hypoxanthine (Hx). During the modeling process, hyperbaric oxygen therapy was also administered. Serum uric acid (UA), creatinine (Cr)and blood urea nitrogen (BUN)levels were measured, and HE staining was used to evaluate the histopathological damage of small intestine and kidney tissues. Serum non-targeted metabolomics analysis was conducted, and 16 S rRNA gene sequencing was used to analyze the changes in intestinal flora structure, to evaluate the therapeutic effect of hyperbaric oxygen therapy. The results showed that HBO therapy could significantly reduce the UA, Cr and BUN levels and alleviate the histopathological damage of small intestine and kidney tissues in model rats. At the same time, HBO therapy could regulate the imbalance of intestinal flora, reduce potential pathogenic bacteria, and reduce the endogenous production of UA by decreasing the relative abundance of Bacteroides and Alistipes . Further serum metabolomics analysis indicated that HBO therapy could improve the differential metabolites in HUA rats, and the pathways of these differential metabolites were mainly related to glutathione metabolism, as well as d -glutamine and d -glutamate metabolism. HBO therapy could regulate key pathways such as purine metabolism and amino acid metabolism, and promote the generation of UA excretion-related metabolites. This study confirmed that HBO could improve combined drug-induced HUA by remodeling the intestinal flora structure and regulating the host metabolic pathways, providing new experimental evidence and potential targets for non-pharmacological treatment of HUA.
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PHYTOMEDICINE
·
2026
·
IF 8.3
Guosen Ou,Jialin Wu,Shiqi Wang,Wensong Bi,Rui Peng,Pei Liu,Yawen Jiang,Yaokang Chen,Huachong Xu,Li Deng,Huan Zhao,Xiaoyin Chen,Lu Xu
Background : Plantago asiatica L. (PTGA) is a widely used herbal medicine for the treatment of gout and hyperuricemia (HUA). Emerging evidence highlights the pivotal role of the gut microbiota in the pathogenesis of gout and HUA. However, existing research has failed to identify and verify the key mediator strains of PTGA that exert its role in lowering uric acid. Methods : A hyperuricemia mouse model was established by intraperitoneal co-administration of hypoxanthine (100 mg/kg) combined with potassium oxonate (50 mg/kg) daily for 10 consecutive days. Serum uric acid (sUA) levels and renal function parameters were assessed using biochemical assay kits. 16S rRNA sequencing combined with non-targeted metabolomics was employed to characterize alterations in gut microbiota and intestinal metabolites. Western blotting was performed to examine the expression of intestinal and renal uric acid transporters, intestinal tight junction proteins, and NLRP3 inflammasome-related proteins. Finally, the mediate role of gut microbiota was verified through fecal microbiota transplantation (FMT) and oral supplementation with Lachnospiraceae bacterium . Results : In the HUA model, elevated sUA levels ( P <0.01), activation of the renal NLRP3 inflammasome ( P <0.05), renal edema, and impaired renal function were accompanied by gut microbiota dysbiosis. PTGA extract markedly reduced sUA levels by approximately 70% compared to the model group ( P < 0.01), regulated uric acid transporter expression in both the intestine and kidney ( P <0.05), and reshaped gut microbiota composition. Moreover, PTGA enhanced intestinal uric acid catabolism of uric acid in the intestine. FMT and Lachnospiraceae bacterium supplementation experiments further confirmed the regulation of the gut microbiota is a key mediator of PTGA’s therapeutic efficacy. Conclusion : This study demonstrates that PTGA exerts hypouricemic and renoprotective effects through modulation of the gut-kidney axis by enriching Lachnospiraceae , promoting intestinal uric acid catabolism, and suppressing renal NLRP3 inflammasome activation. These findings provide novel mechanistic insights into the gut microbiota-dependent therapeutic action of herbal medicine, distinguishing this work from previous studies focused solely on direct organ-level effects.
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Journal of Pharmaceutical Innovation
·
2026
·Vol. 21
·期 4
·340
·
IF 2.6
Hou Bo,Ma Dewei,Yang Keke,Peng Mingwei,Liu Qinglong,Heng Ziwen,Chen Siyuan,Liu Wei
Purpose Retinol (RET) and niacinamide (NA) are widely used in anti-aging and skin-brightening skincare products. However, RET is unstable under light and oxygen, while NA exhibits poor skin permeation. This study aimed to develop a liposomal co-delivery system (RN-Lip) to overcome these limitations and enhance the synergistic efficacy of the two compounds. Method RN-Lip was prepared by lipid film hydration, followed by extrusion to decrease particle size. The liposomal formulation was optimized, and its stability was evaluated under various conditions. Additionally, in vitro transdermal delivery, cellular uptake, cytotoxicity, and cellular efficacy evaluations were performed. Results The optimized RN-Lip exhibited a particle size of 147.03 ± 3.76 nm. The encapsulation efficiencies (EE) for NA and RET were 19.47% and 41.67%, respectively. Liposomal encapsulation improved the stability of RET, with a 7-fold higher retention rate than free RET after 30 days of storage at room temperature (under light protection). Compared to free NA and RET, liposomal encapsulation increased the skin retention of NA and RET by 3.8-fold and 1.5-fold, respectively, and further enhanced cellular uptake. Furthermore, RN-Lip reduced melanin content and tyrosinase activity in B16F10 cells compared to the free combination of RET and NA, promoted collagen I synthesis in BJ cells, and protected HaCaT cells against H₂O₂-induced oxidative damage. Conclusion RN-Lip successfully co-delivered RET and NA, overcoming their individual limitations and boosting their skincare functionalities. Thus, this system holds significant potential for advanced anti-aging and skin-brightening applications.
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