MOLECULES
·
2025
·Vol. 30
·期 12
·2560
·
IF 4.6
Zihao Wang,Xiao Liu,Yifan Ma,Jiaxin Zheng,Ke Xu,Yingxue Chang,Zhaoyan Ye,Yong Ling,Lei Wang
To address the clinical challenges posed by symbiotic drug-resistant bacterial infections and tumor microenvironments, this study designed and synthesized novel carbazole/benzindole-based photosensitizersA1–A4, systematically evaluating their antitumor and antibacterial therapeutic potential through chemo-photodynamic therapy. Especially, compoundA4demonstrated potent Type I/II reactive oxygen species (ROS) generation capabilities. In vitro experiments revealed thatA4concentration-dependently inhibited HT-29 cells under hypoxic conditions (IC50= 0.89 μM) with a prominent photodynamic index (PI > 9.23), and substantially promoted cancer cell programmed death. In antibacterial evaluations,A4achieved the complete eradication of dermal MRSA infections within 7 days through ROS-mediated membrane disruption under illumination. In the HT-29 xenograft model, the PDT–chemotherapy synergy strategy achieved a tumor suppression rate of 96%. This work establishes an innovative strategy for the combinatorial management of multidrug-resistant infections and solid tumors.
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ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Huiyu Zou,Yuying Zheng,Erkang Bian,Chenyang Li,Zhengwu Yang,Zhiyu He,Jingjing Zhou,Chunyan Deng
Early diagnosis of cardiovascular diseases requires precise detection of multiple biomarkers, yet simultaneous analysis of these biomarkers without cross-talk remains a major challenge. To overcome this limitation, we developed a novel dual-zone biosensing platform capable of generating independent multimode signals for the parallel detection of cardiac troponin I (cTnI, the gold-standard biomarker) and interleukin-6 (IL-6, a key inflammatory marker). This platform features two spatially resolved zones, Zone I integrates rhodamine B (RhB) with a cadmium sulfide@bismuth tungstate (CdS@Bi2WO6) composite, allowing dual-signal detection of cTnI via photoelectrochemical (PEC) and smartphone-based fluorescent (FL) methods. Zone II is functionalized with dsDNA/methylene blue (MB), enabling dual-signal detection of IL-6 through PEC and electrochemical (EC) techniques. The biosensor exhibits excellent analytical performance, including high specificity against interfering species, a broad dynamic range covering clinically relevant concentrations, and high detection sensitivity (cTnI: 1 ∼ 50,000 pg mL–1 for PEC, 10 ∼ 50,000 pg mL–1 for FL; IL-6:1 ∼ 20,000 pg mL–1 for PEC, 1 ∼ 20,000 pg mL–1 for EC.) with low limits of detection (cTnI: 0.27 pg mL–1 for PEC, 0.86 pg mL–1 for FL; IL-6:0.57 pg mL–1 for PEC, 0.65 pg mL–1 for EC.). Importantly, it maintains robust functionality in human serum and shows strong correlation with established clinical methods. This work provides a generalizable framework for developing multiplexed biosensors that combine orthogonal detection principles, effectively addressing key challenges in multicomponent bioanalysis, which is a significant advancement in the field of clinical diagnostics.
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WATER SCIENCE AND TECHNOLOGY
·
2018
·Vol. 78
·期 6
·1390-1399
·
IF 1.247
Li Wenshu,Zhou Peng,Zhang Jing,Zhang Yongli,Zhang Gucheng,Liu Yang,Cheng Xin
This study investigated the generation of reactive oxygen species (ROS) (O 2 − •, H 2 O 2 , and HO•) by promoting the Cu(II)/Cu(I) redox cycle with certain reducing agents (RAs) in aerobic aqueous solution, and benzoic acid (BA) was employed as indicator for the hydroxyl radical (HO•). Hydroxylamine (HA) can reduce Cu(II) to Cu(I) to induce chain reactions of copper species resulting in the generation of the superoxide radical (O 2 − •) and hydrogen peroxide (H 2 O 2 ), and the intermediate Cu(I) can further activate H 2 O 2 via a Fenton-like reaction to produce HO•, creating the remarkable BA degradation. O 2 is indispensable, and unprotonated HA is the motive power in the O 2 /Cu/HA system. Moreover, pH is a crucial factor of the O 2 /Cu/HA system due to the protonated HA not being able to reduce Cu(II) into Cu(I). The oxidation of HA can be effectively induced by trace amounts of Cu(II), and both a higher HA dosage and a higher Cu(II) dosage can enhance H 2 O 2 generation and BA degradation. In addition, some other RAs that can reduce Cu(II) into Cu(I) could replace HA in the O 2 /Cu/HA system to induce the generation of these ROS in aerobic aqueous solution.
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Materials Today Bio
·
2026
·
IF 11
Shanlingzi Huang,Lu Gao,Yujun Chen,Zhaoming Fu,Ziyou Wang,Yifan Liu,Zhicheng Zhou,Ru Huang,Wen Song,Feifan Zhou
Triple-negative breast cancer (TNBC), characterized by low immunogenicity, is a challenging issue in clinical treatment due to its poor response to various therapies. The transformation of TNBC from a “cold tumor” with low immunogenicity into a “hot tumor” that elicits stronger immune responses is a key research focus. Photodynamic therapy (PDT) has emerged as a promising solution for TNBC treatment because it can effectively induce immunogenic cell death (ICD), which prompts the release of damage-associated molecular patterns (DAMPs) and activates immune responses. The role of MHC-I molecules in antigen presentation is crucial, but TNBC cells often evade immune surveillance by downregulating or losing MHC-I expression. Recent studies have shown that inhibiting the activity of complex II (CII) in the mitochondrial electron transport chain of tumor cells can promote MHC-I expression. Based on this finding, the combination of the PDT photosensitizer PCN-224 with the CII inhibitor 3-nitropropionic acid (3NPA) to form PCN@3NPA has been innovatively developed. Furthermore, modification with hyaluronic acid (HA) enables targeted delivery to TNBC cells that overexpress CD44. PDT induces ICD in tumor cells, releasing large amounts of DAMPs, while the sustained release of 3NPA effectively inhibits CII activity, significantly enhances MHC-I expression, thereby boosting tumor cell immunogenicity. This process significantly improves the recognition and killing of tumor cells by CD8 + T cells, markedly strengthening the body's anti-tumor immune response. This approach offers a novel and promising strategy for efficiently treating not only low-immunogenic TNBC but also other similar tumors, bringing new hope for future clinical treatments.
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OPTICAL MATERIALS
·
2023
·
IF 3.9
Shun Lu,Kegui Li,Ruojing Zhen,Maling Xiang,Xiaoyan Gan,Liling Guo,Hanxing Liu
Recently, perovskite solar cells (PSCs) basing on quasi-two-dimensional (quasi-2D) perovskites with hydrophobic bulky organic cations have received a lot of interests owing to their superior moisture stability. While quasi-2D perovskites have achieved great success in planar p-i-n PSCs, their application in planar n-i-p devices and the corresponding efficiencies lag far behind. In our work, (FPEA) 2 (MA) 4 Pb 5 I 16 perovskite films with high crystallinity , compact and smooth surface were deposited on electron transport layer with NH 4 SCN as an additive. Formamidinium (FA + ) were then incorporated into the FPEA based perovskite films to partially replace methylammonium (MA + ), and effects of FA + on the film's vertical orientation, light absorption capacity, carrier lifetime, defect density and electron mobility were studied. It was found that when molar ratio of FA/MA = 0.5/9.5, the film had the highest vertical orientation, enhanced light absorption, less carrier recombination loss. The obtained (FPEA) 2 (FA 0.05 MA 0.95 ) 4 Pb 5 I 16 based n-i-p PSCs gave a maximum efficiency of 12.17% under standard illumination, retaining 83% of the initial efficiency after stored in air environment (25–30 °C) with 40 ± 5% RH for 1080 h. The results demonstrate an efficient approach to prepare high-quality quasi-2D perovskite films in a n-i-p structure as well as improved device efficiency and stability.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xiaozong Dou,Zhenhong Jia,Hongyan Zhang,Chu Chen,Ling Zhang,Jianping Man,Weiyuan Gu
A high-performance fluorescent probe 2,5-dimercapto-1,3,4-thiadiazole copper nanoparticles (DMTD-CuNPs) was synthesized by hydrothermal method based on monovalent copper (Cu(I)) and 2,5-dimercapto-1,3,4-thiadiazole (DMTD), and it can effectively detect cysteine (Cys) in plasma. Experiments show that DMTD can reduces band gap of Cu(I) in DMTD-CuNPs, promote charge transfer transition from DMTD to Cu(I) and significantly enhance fluorescence intensity of DMTD-CuNPs at 515 nm. The large Stokes shift of DMTD-CuNPs is 315 nm, which can reduce the self-quenching of probe fluorescence and improves detection accuracy of the probe. In the presence of Cys, fluorescence of DMTD-CuNPs at 515 nm is significantly quenched because Cys reacts with Cu(I) in DMTD-CuNPs through Cu-S bond to form reduced charge transfer, which can be successfully used for the detection of Cys. Linear range and detection limit for Cys detection are 25–65 µM and 50 nM, respectively. Furthermore, feasibility of detecting Cys in plasma using DMTD-CuNPs probe was evaluated by standard addition method, and the absolute recovery is 96–99%. Such a DMTD-CuNPs probe shows high sensitivity, good selectivity and low detection limit for Cys, which is expected to be used for the practical analysis of Cys in plasma.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Ziwen Tao,Guotao Yi,Chao Zeng,Qianfen Zhuang,Yong Wang
Silver ion (Ag + ) is one of the most toxic and dangerous heavy metal pollutants. Therefore, finding a simple, sensitive and selective approach to detect Ag + is very urgent for environmental protection and risk reduction to human health. In this work, water-soluble red-emitting micellized phosphorescent silver nanoclusters (Ag 29 @Na micelles) with a large stokes shift of 380 nm are prepared and used as sensitive and selective phosphorescent probes to realize the rapid and simple detection of Ag + . Upon adding Ag + , the phosphorescence of Ag 29 @Na micelles is quenching, and the phosphorescence quenching ratio is linearly proportional to the Ag + concentration over the range from 1 nM to 100 nM with the detection limit of 0.8 nM ( S / N = 3). The Ag 29 @Na micelles exhibit high selectivity for Ag + over other potentially interfering metal ions . The quenching mechanism of Ag 29 @Na micelles by Ag + is studied in details using various analytical characterizations including UV–vis absorption spectroscopy, photoluminescence lifetime, X-ray photoelectron spectroscopy, transmission electron microscopy , and zeta potential , and the results denote that the aggregation between Ag 29 @Na micelles and Ag + is responsible for the phosphorescence quenching. The Ag 29 @Na micelles-based phosphorescence probes are successfully applied to detect Ag + in lake water samples with good recoveries of 98 %−108 %.
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Foods
·
2021
·Vol. 10
·期 12
·2896
·
IF 4.35
Liangliang Zhu,Hongshun Hao,Chao Ding,Hanwei Gan,Shuting Jiang,Gongliang Zhang,Jingran Bi,Shuang Yan,Hongman Hou
To achieve the rapid detection ofListeria monocytogenes, this study used aptamers for the original identification and built a photoelectrochemical aptamer sensor using exonuclease-assisted amplification. Tungsten trioxide (WO3) was used as a photosensitive material, was modified with gold nanoparticles to immobilize complementary DNA, and amplified the signal by means of the sensitization effect of CdTe quantum dots and the shearing effect of Exonuclease I (Exo I) to achieve high-sensitivity detection. This strategy had a detection limit of 45 CFU/mL in the concentration range of 1.3 × 101–1.3 × 107CFU/mL. The construction strategy provides a new way to detectListeria monocytogenes.Keywords:photoelectrochemical detection;aptamer;Listeria monocytogenes;quantum dots;exonuclease I
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JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 17
·3279-3286
·
IF 3.645
Youqing Sun,Yugao Guo,Jianpo Ren,Lieyi Ji,Qingyin Zhang
By replacing the permanent magnet with an electromagnet, traditional magnetic solid-phase extraction was developed into electromagnetic dispersive solid-phase extraction. A simple operation of power on and off can realize the separation of adsorbents from solutions easily. The improvement makes it possible for the automation of the determination of Sudan Red I by high-performance liquid chromatography. After series of optimization, a trace amount of Sudan red I was well-determined, and excellent linearity was achieved in the range of 0.005 to 1 mg/L with the correlation coefficient ( R 2 ) = 0.997. The limit of detection with a signal-to-noise ratio of 3 was found to be 0.001 mg/L. The spiked recoveries of Sudan red I for the samples ranged from 93.6 to 104.9%. Moreover, the adsorbent could be recycled at least ten times. The results show that the electromagnetic dispersive solid-phase extraction combined with high-performance liquid chromatography is a rapid, eco-friendly, effective, and sensitive determination method with fascinating automation potential and high practical applicability.
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FOOD CHEMISTRY
·
2021
·
IF 7.514
Yuncong Xu,Ling Zhao,Ling Chen,Yali Du,Yuanyuan Lu,Chen Luo,Yijun Chen,Xuri Wu
A new compound, α-siamenoside I (α-SI), with a glucose unit selectively bound to the 6-hydroxyl group of the 24- O -β-glucosyl moiety of mogroside IIIE by α-1,6-glucosidic bond, was bio-created by two screened cyclodextrin glycosyltransferases with a maximum yield of 59.3%. Compared to mogroside IIIE, α-SI showed a significantly increased sweetness intensity (508 times sweeter than 5% sucrose), which is superior to siamenoside I (SI), the sweetest triterpenoid saponin isolated from Siraitia grosvenorii to date. Sensory evaluation showed that the taste quality of α-SI also was obviously better than mogroside IIIE. In addition to α-SI possessing a good stability similar to that of SI, it also did not cause a significant decrease in cell viability at a concentration of 200 μg/mL and had a negative influence on islets function at 1 μM. All of these preliminarily results pave the way for promoting α-SI as a potential low-calorie sweetener.
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MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
Ye Jianping,Cai Xin,Zhou Qing,Yan Zhihong,Li Kang
A molecularly imprinted ratiometric fluorescent probe (MIRF probe) was synthesized for the determination of aristolochic acid I (AAI) based on the Schiff-base fluorescent compound N,N′-bis(o-carboxybenzylidene)-p-4,4′-diaminobiphenyl (BDDB). The BDDB was immobilized in the silica nanoparticle (BDDB@SiO 2 ) as an internal standard material. The blue-emitting BDDB@SiO 2 and the yellow-emitting carbon quantum dots (y-CDs) were wrapped in the molecularly imprinted polymer (MIP) to provide a reliable reference signal at 440 nm and a fluorescent response signal at 530 nm at the excitation wavelength of 365 nm, respectively. In the preparation of the MIP of the MIRF probe, 4-vinylbenzoic acid as the functional monomer and AAI as the template molecule were used. An imprinting factor of 2.25 was obtained. Under the optimum conditions, the fluorescent response signal at 530 nm was quenched gradually by AAI in the range 1.0 to 120.0 μmol/L, while the reference signal at 440 nm remained unchanged. The limit of detection was 0.45 μmol/L, and the fluorescent color of the MIRF probe changed gradually from yellow to green to blue, which illustrated that the developed probe had a specific AAI recognition ability, a good anti-interference ability, and a sensitively visual determination ability. The probe was successfully applied to the AAI determination in traditional Chinese medicine (TCM) Asarum . The results showed that it had satisfactory recoveries (95.5–107.3%) and low relative standard deviations (2.0%). Furthermore, this method has a potential for the onsite naked eye determination of AAI in TCM samples. Graphical abstract
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JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Siqi Zhuo,Yan Liu,Wei Li,Zhi Ding,Mingda Li,Qianjin Li,Xin Wang,Jie Liu,Rui Shao,Qianqian Ling,Tiesong Zheng,Jianlin Li
Ordered porous materials are attracting enormous attention due to their uniform pore structures, particularly the magnetic photonic crystal microspheres (PCMs) which not only possess unique photonic crystal structure but also can achieve separation easily based on magnet. Here, a two-phase microfluidic self-assembly synthetic system was established simply and employed for the preparation of three dimensional PCMs (3DPCMs) by using the emulsion droplet approach. One phase (dispersed phase) was an aqueous emulsion containing Fe 3 O 4 , silica (SiO 2 ) and polystyrene (PS) nanoparticles; another phase (continuous phase) was pure silicone oil. The droplets were formed by introducing the dispersed phase into the continuous phase through a tee valve. By heating the droplets, the water would evaporate and the nanoparticles would finally assemble into solid microspheres, which could be changed into macroporous 3DPCMs after removal of the PS nanoparticles by calcination. The contents and particle sizes of Fe 3 O 4 , SiO 2 and PS nanoparticles in the dispersed phase were investigated in detail and optimized to prepare macroporous magnetic 3DPCMs with high quality. The morphologies, surface crystal structure, magnetic property, particle size distribution, specific surface area and pore size of the macroporous magnetic 3DPCMs were characterized. The expected 3DPCM displayed regular and uniform photonic crystal structure, narrow particle size distribution and strong magnetic property. The macroporous magnetic 3DPCMs grafted with vomitoxin (DON)-antibodies could be applied for selective enrichment of DON in real samples.
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