Cell Reports Methods
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2024
·
IF 4.3
Jinli Geng,Yaxiong Yang,Boying Li,Zhen Yu,Shuang Qiu,Wen Zhang,Shixin Gao,Nan Liu,Yi Liu,Bo Wang,Yubo Fan,Chengfen Xing,Xiaodong Liu
Genetically encoded inhibitors of Ca V 1 channels that operate via C-terminus-mediated inhibition (CMI) have been actively pursued. Here, we advance the design of CMI peptides by proposing a membrane-anchoring tag that is sufficient to link the inhibitory modules to the target channel as well as chemical and optogenetic modes of system control. We designed and implemented the constitutive and inducible CMI modules with appropriate dynamic ranges for the short and long variants of Ca V 1.3, both naturally occurring in neurons. Upon optical (near-infrared-responsive nanoparticles) and/or chemical (rapamycin) induction of FRB/FKBP binding, the designed peptides translocated onto the membrane via FRB-Ras, where the physical linkage requirement for CMI could be satisfied. The peptides robustly produced acute, potent, and specific inhibitions on both recombinant and neuronal Ca V 1 activities, including Ca 2+ influx-neuritogenesis coupling. Validated through opto-chemogenetic induction, this prototype demonstrates Ca 2+ channel modulation via membrane-assisted molecular linkage, promising broad applicability to diverse membrane proteins.
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Natural Product Communications
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2025
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IF 1.4
Phuong Nhung Nguyen,Ngoc Lien Nguyen,Hai Nam Pham,Phuong Khanh Nguyen,Kieu Oanh Nguyen Thi
ObjectivesPteris vittata L. is one of the hyperaccumulator plants that can grow in heavy metal-contaminated soil. This plant may tolerate metal stress through different mechanisms, eg, by producing a wide range of secondary metabolites. These compounds play an essential role in physiological function and can also serve as rich sources of chemicals for health benefit purposes. This study aims to determine the phytochemicals of plant extracts and the relationship between these profiles with pharmacological properties to predict active agents and their interaction.MethodsThe total phenolic and total flavonoid contents were measured by colorimetric assays, while the widely targeted metabolomics was applied to reveal leaf and root P. vittata metabolome. In parallel, the antioxidant and antibacterial effects of these extracts were determined by ABTS scavenging and micro-broth dilution methods. Multivariate analysis, ie, Partial Least Square Regression, calculated the correlation between metabolites and bioactivities.ResultsThe results showed the significant content of phenolics and flavonoids in plants collected from mining soils of Thai Nguyen province, Vietnam, and their potential ABTS scavenging activity. Additionally, the plant's metabolome characterized 82 compounds, mainly secondary metabolites. The multivariate analysis indicated the positive correlation among quercetin derivatives and bioactivity, suggesting the contribution of their combination, not individually, to the antibacterial effect of P. vittata.ConclusionHyperaccumulators in metalliferous soils are potential reservoirs for exploring the antibacterial agents against multi-drug resistant strains, and this effect may be exerted by additive or synergistic mechanisms of action of multiple compounds.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
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2022
·Vol. 31
·期 1
·1-12
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IF 4.705
Yuan Yuka,Fu Zhe,Liu Yudong,Liu Kai,Hua Jing
Poly (L-lactic acid) (PLLA) suggests attractive biomedical applications in drug releasing system, surgical suture, bone fixing and tissue repairing due to its degradable and biocompatible benefits. However, currently developed catalysts with high toxicity and currently reported PLLA with uncontrollable crystallinity are detrimental for the environmental protection and human health. Herein, we proposed a novel and effective approach to synthesizing PLLA using environmentally-friendly bio-based phenol-substituted triisobutylaluminum as the catalyst. The effects of the chemical structures of various bio-based phenols on the polymerization activity and stereoregularity of PLLA were explored in detail. The increased steric hindrance of bio-based phenols was beneficial for the polymerization activity of PLLA. The resultant PLLA exhibited an ultrahigh monomer conversion efficiency up to 98.32% among the highest value of reported PLLA. Moreover, the PLLA also demonstrated quick polymerization rate (< 5 h) and low polymerization temperature (< 100 °C), with controllable polymer crystallinity (36 ~ 46%) and number average molecular weight (8400 ~ 16,000 g mol −1 ). Consequently, the strategy and results collectively represent a significant advance toward the preparation of high-performance degradable and biocompatible PLLA.
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Journal of Macromolecular Science Part A-Pure and Applied Chemistry
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2013
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IF 0.807
Shi-Ping Zhan,Xing Huang,Qi-Cheng Zhao,Li Chen,Wei-Min Hou,Song Zhang,Jin-Jun Deng
Dispersion polymerization of lactides and lactones has been studied and has received a great deal of attention used to prepare biodegradable polymers in supercritical carbon dioxide (ScCO2). The triblock copolymers of poly(ϵ-caprolactone) PCL-polydimethylsiloxane (PDMS)-poly(ϵ-caprolactone) PCL by the feed ratio of 1:2:1 and 1:3:1, respectively were synthesized to be used as the stabilizers in the dispersion polymerization of L-lactide. The fine poly(L-lactide) (PLLA) powders were obtained at the concentrations of 3% to 5%w/w (stabilizer/monomer). The results demonstrated that the triblock copolymers were the effective stabilizers for the dispersion polymerization of L-lactide. It was also found that the cooling process had a significant effect on the particle size of the fine powders. In order to obtain the best PLLA powders, it would be necessary to use the cooling process with stirring.
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Analytical Methods
·
2025
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IF 2.6
Minzi Li,Hongying Wang,Shaohong Zhang,Ran Zhang,Zhijuan Wang
A trimetallic (Fe, Co, Zn) nanozyme embedded within a N-doped carbon matrix (FeCoZn-NC-1.5-800) was engineered via coordination-controlled pyrolysis of Fe ions doped core-shell ZIF-8@ZIF-67 (ZIF-8@ZIF-67-Fe-1.5) precursor, which exhibited dual oxidase/peroxidase-mimicking activities. The bifunctional catalyst oxidized 3,3',5,5'-tetramethylbenzidine (TMB) to form a blue product of oxidized TMB (oxTMB) without exogenous H2O2. However, the addition of L-cysteine (L-Cys) acted as a potent inhibitor, quenching oxTMB formation via thiol-mediated electron transfer, leading to a significant decrease in absorbance and the fading of the blue colour to colourless. Consequently, an H2O2-independent colormetric biosensor was developed with a wide linear range (5-50 μM), a low detection limit (0.569 μM, 3σ), good selectivity and anti-interference ability. This work establishes a paradigm for transition metal nanozyme design, offering clinical translation potential in point-of-care thiol monitoring and biocatalytic system development.
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PubMed
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CHEMICAL ENGINEERING JOURNAL
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2025
·
IF 13.2
Ang Xu,Jie Gao,Huan Li,Xiyu Song,Bo Xu,Ying Liu,Yuanyuan Yu,Ping Wang,Qiang Wang,Man Zhou
Achieving a high working voltage window beyond the water electrolysis limit (1.23 V) is always a challenge for aqueous supercapacitors (SCs). Increasing the oxygen evolution reaction (OER) overpotential or hydrogen evolution reaction (HER) overpotential of the carbon-based electrode is an effective way to broaden the voltage window of SCs. In this work, we propose an efficient strategy of surface engineering to autonomously regulate the pH of the electrode/electrolyte interface microenvironment by using the amphoteric dissociation of amino acids, which suppress the OER and consequently broadens voltage window. L-Lysine was modified on the surface of carbon cloth to prepare the SC positive electrode. The voltage window of SCs based on L-Lysine modified electrode is enhanced from 1.2 V to 1.5 V and the energy density has increased by 59.42 % at 0.5 A g −1 . This is a promising strategy to design and engineer carbon-based aqueous SCs with a boosted energy density.
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STARCH-STARKE
·
2025
·
IF 2.7
Yanqu Cai,Haotian Chen,Yao Wen,Haiyun Chen
To contribute to the development of natural plants in the field of medicine, the study investigates optimization of the best process of extracting polysaccharides of Eleusine indica (L.) Gaertn polysaccharide (EIGP) with water as a solvent and the bioactivity of EIGP in vitro. EIGPs were extracted by an ultrasonic-assisted method using water as a solvent; a one-factor experiment was carried out first, and then a response surface experiment was designed according to the four-factor and three-level design by using the Design-Expert software. The polysaccharide extract of Eleusine indica (L.) Gaertn (EIG) was eluted with a DEAE column and Sephadex G-75 column to obtain the purity of polysaccharides. The purified polysaccharide was then subjected to preliminary antioxidant and hypoglycemic activities by enzyme inhibition and in vitro free radical scavenging assays. The optimal extraction conditions were ultrasonic power of 240 W, solid–liquid ratio of 1:17 g/mL, time of 31 min, and temperature of 50°C, under which the polysaccharide extraction rate of EIG was 11.50% and the purity was 73.45%. In the bioactivity study of polysaccharides, the results were as follows: in the α-glucosidase inhibitory activity assay, the IC 50 of these polysaccharides and acarbose were measured to be 59.84 and 4.4 × 10 −4 mg/mL, respectively; in the α-amylase inhibitory activity assay, the IC 50 of these polysaccharides and acarbose were measured to be 59.98 and 0.2173 mg/mL; in the DPPH• radical scavenging activity assay, the IC 50 of the polysaccharide and Vitamin C (VitC) were measured to be 43.95 and 0.0126 mg/mL, respectively; in the ABTS• + scavenging assay, the IC 50 of the polysaccharide and VitC were measured to be 0.4188 and 0.004 mg/mL, respectively. In this thesis, the process of extracting polysaccharides from EIG with water as a solvent was investigated and optimized. The bioactivity study experiments showed that EIGPs-1, purified from EIG, have certain antioxidant and hypoglycemic activity.
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LABORATORY INVESTIGATION
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2022
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IF 5.502
Ruo-Peng Liang,Xiao-Xue Zhang,Jie Zhao,Rong-Tao Zhu,Wei-Jie Wang,Qin-Wei Lu,Yu-Ling Sun
Ubiquitin-specific protease 3 (USP3), a kind of cysteine protease, is a crucial family member of deubiquitinating enzymes. USP3 is aberrantly expressed in several tumors, which may contribute to cancer progression. However, the role of USP3 in gallbladder cancer (GBC) is still unknown. In the current study, we detected the expression of USP3 in GBC tissues, measured its contribution to the cell proliferation in GBC progression, and further studied the underlying mechanism of USP3 in GBC through pyruvate kinase L/R (PKLR; a kind of glycolytic enzyme). We found that the expression of USP3 in GBC tissues were higher than that of adjacent tissues, and the protein levels of USP3 and PKLR were positively correlated. Additionally, overexpressed USP3 significantly promoted cell proliferation in vitro and tumor growth in vivo, while the silencing of USP3 inhibited proliferation and tumor growth. Glycolysis in GBC cells ws promoted by the USP3 overexpression and inhibited bye USP3 downregulation. Moreover, the loss of USP3 promoted the ubiquitination and weakened the stability of PKLR. Results of the rescue assay confirmed that PKLR knockdown suppressed USP3-induced oncogenic activity in USP3 overexpressed GBC cells. These findings imply that USP3 is an essential positive regulator in GBC progression, and USP3-PKLR plays a vital role in the progression and metabolism of GBC. Ubiquitin-specific protease 3 (USP3), a cysteine protease, is deubiquitinating enzyme. USP3 is aberrantly expressed in several types of tumors. The authors show that USP3 is an important positive regulator in gallbladder cancer progression, and that pyruvate kinase L/R plays a key role in the progression of GBC.
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Journal of Macromolecular Science Part B-Physics
·
2019
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IF 1.204
Wei Wu,Zhihua Zhou,Wenjuan Liu,Yunhui Zhao,Yanmin Zhao,Tianlong Huang,Xiaofei Li,Jianjun Fang
A random terpolymer of poly(L-lactide-co-glycolide-co-ε-caprolactone) (PLLGC) was synthesized from L-lactide, glycolide and ε-caprolactone. The in-vitro hydrolytic degradation behavior of the PLLGC terpolymer was investigated as a function of the degradation time; the results showed that the degradation rate of PLLGC was lower than that of PLGA due to a reduction in the acidic degradation products. Therefore, we suggest the degradation rate of the PLGA could be controllable by introduction of ε-caprolactone into the PLGA martrix, while the physical properties (mechanical, etc.) were superior to PLGA.
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GENETIC RESOURCES AND CROP EVOLUTION
·
2013
·Vol. 60
·期 8
·2207-2217
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IF 1.593
Mikel Mark A.
A major objective of this work is to elucidate the ancestry and genetic background of contemporary U.S. garden pea ( Pisum sativum L. convar. medullare Alef.) cultivars used as cultivated vegetables. This is facilitated through pedigree analysis of 147 cultivars registered during the era from 1990 through 2010 by collecting data from U.S. Plant Variety Protection, U.S. utility patent, and journal publication. Proprietary breeding programs developed 141 cultivars and public programs six, with over half (82 of 147 cultivars) originating from the two (Seminis Vegetable Seeds, Inc. and Syngenta Seeds, Inc.) largest proprietary breeding programs. During this era, the semi-leafless (afila) trait was bred into cultivars to decrease lodging, and 43 % of the cultivars were afila. Registered cultivars were 86 % of determinate growth habit. Bi-parental breeding crosses were used in the development of 78 % of these cultivars. The lineages of the cultivars ‘Bolero’, ‘Spring’, ‘Genie’, and ‘Rally’ made large contributions to the genetic composition of this germplasm, ‘Bolero’ contributed 6.5 % (29 progeny), ‘Spring’ 6.4 % (29), ‘Genie’ 4.0 % (18), and ‘Rally’ 3.6 % (23) of the genes. The garden pea ancestor cultivar ‘Perfection’ fostered the most descendants (75) among the 147 cultivars of this era.
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JOURNAL OF SOLUTION CHEMISTRY
·
2017
·Vol. 46
·期 5
·1048-1058
·
IF 1.342
Li Qiang,Liu Bijun,Tian Yin,Yu Qianhong,Mu Wanjun,Wei Hongyuan,Wang Dongqi,Li Xingliang,Luo Shunzhong
N -(phosphonomethyl)- l -proline is an analogue of glyphosate. The protonation for N -(phosphonomethyl)- l -proline was studied by potentiometry, calorimetry, 31 P NMR spectroscopy and quantum chemical calculations to further understand the protonation process of glyphosate. The results confirmed that the order of successive protonation sites of totally deprotonated N -(phosphonomethyl)- l -proline are a phosphonate oxygen, amino nitrogen, and finally the carboxylate oxygen. The results can improve the understanding of the biological activity of these types of molecules in solution.
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Carbohydrate Polymer Technologies and Applications
·
2026
·
IF 7.6
Guang-Zhao Wang,Yu-Zhuo Song,Yan Wang,Sheng Lan,Ling-Hong Huang,Xue-Wu Chen,Xin-Yuan Sun
Kidney stone disease is a common pathological mineralization disorder with high recurrence. Plantago asiatica L. has traditionally been used for urinary calculi, but its active constituents and mechanisms remain unclear. Here, a water-soluble polysaccharide from P. asiatica L. (PLP) was isolated, structurally characterized, and evaluated for anti-nephrolithic activity. PLP possessed a glucan-rich backbone mainly composed of →4)-α-D-Glcp-(1→ and →4,6)-α-D-Glcp-(1→ residues, with O-6-linked terminal α-D-Glcp branches and minor β-1,3/1,4-glucan and arabinogalactan moieties. PLP inhibited calcium oxalate (CaOx) nucleation, growth, and aggregation and promoted the transformation of pathogenic CaOx monohydrate into less-adhesive CaOx dihydrate. In renal tubular epithelial cells, PLP reduced reactive oxygen species, preserved mitochondrial membrane potential and lysosomal integrity, and inhibited apoptosis and crystal-adhesion molecule expression. In stone-bearing mice, PLP reduced renal crystal deposition, tubular injury, inflammatory infiltration, and improving renal function. Integrated transcriptomic and untargeted metabolomic analyses showed that PLP suppressed injury- and inflammation-related genes, restored tubular transport, and regulated peroxisomal function, purine and glyoxylate/dicarboxylate metabolism, and the TCA cycle. Collectively, PLP protects against CaOx nephrolithiasis by coordinating crystal modulation with restoration of renal redox and metabolic homeostasis, thereby providing mechanistic support for its development as a natural anti-urolithic candidate.
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