JOURNAL OF MATERIALS RESEARCH
·
2023
·Vol. 38
·期 8
·2213-2224
·
IF 2.7
Zhang Quanchao,Zhong Bingbing,Zhang Yichuan,Yang Zhiwei,Zhu Xiangbo,Wang Jie,Luo Honglin,Wan Yizao
Lack of bioactivity limits the applications of bacterial cellulose (BC) in biomedical fields. In this study, we report the facile preparation of a macroporous BC (PBC)/chondroitin sulfate (CS) scaffold using the ex situ method by adding CS solution into the suspension of BC fragments followed by crosslinking with 1-ethyl-3(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) and freeze drying. The PBC/CS scaffold was characterized for morphology, physicochemical properties, cell behavior, and capability of inducing mineral deposition. Results show that the PBC/CS scaffold presents improved mechanical properties, cell adhesion, and proliferation over the PBC scaffold. Moreover, the presence of CS greatly enhances the deposition of minerals on the PBC/CS scaffold, an indicator of bioactivity. The present study provides a simple methodology for improving the bioactivity of BC and the results of the present work suggest that the PBC/CS scaffold has potential for use in bone tissue engineering. Graphical Abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2016
·Vol. 408
·期 21
·5737-5743
·
IF 3.125
Mei Xiao-Qi,He Xiu-Ping,Wang Jiang-Tao
A kind of new molecularly imprinted polymer (MIP) was synthesized by bulk polymerization using guanosine as dummy template molecule, α-methacrylic acid as functional monomer and ethylene glycol dimethyl acrylic ester as crosslinker. Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) showed that the MIP had homogenous and uniform-sized cavities. It was confirmed that the MIP had higher binding affinity and selectivity towards gonyautoxins 1,4 (GTX 1,4) than the non-imprinted polymer (NIP) according to the static equilibrium adsorption. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with fluorescence detection (HPLC-FLD) was established for the analysis of GTX 1,4. 0.1 mol/L acetic acid and 95:5 (v:v) methanol/water were optimized as the washing and elution solutions, respectively. The recoveries of spiked cultured seawater samples were satisfactory, as high as 88 %. Using this method, the concentrations of GTX 1,4 from cultured seawater samples of Alexandrium minutum and Alexandrium tamarense were detected to be 1.10 μg/L and 0.99 μg/L, respectively.
The synthesis of molecularly imprinted polymer and molecularly imprinted solid-phase extraction analysis for gonyautoxin 1,4
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MEDICINAL CHEMISTRY RESEARCH
·
2014
·Vol. 23
·期 6
·3178-3186
·
IF 0
Yang Zhongduo,Song Zhuwen,Xue Weiwei,Sheng Jie,Shu Zongmei,Shi Yin,Liang Jibei,Yao Xiaojun
Acetylcholinesterase inhibitors (AChEIs) are currently the best available pharmacotherapy for Alzheimer patients, but because of bioavailability issues, there is still great interest in discovering better AChEIs. The aporphine alkaloid is an important class of natural products, which shows diverse biological activity, such as acetylcholinesterase inhibitory activity. To find new lead AChEIs compounds, eight aporphine alkaloids were synthesized by O -dealkylation, N -dealkylation, and ring aromatization reactions using nuciferine as raw material. The anti-acetylcholinesterase activity of synthesized compounds was measured using modified Ellman’s method. The results showed that some synthesized compounds exhibited higher affinity to AChE than the parent compound nuciferine. Among these compounds, 1,2-dihydroxyaporphine ( 2 ) and dehydronuciferine ( 5 ) were the most active compounds (IC 50 = 28 and 25 μg/mL, respectively). Preliminary analysis of structure–activity relationships suggested that aromatization of the C ring, the presence of the alkoxyl group at C1 and the hydroxy group at C2 position as well as the alkyl substituent at the N atom were favorable to the acetylcholinesterase inhibition. Molecular docking was also applied to predict the binding modes of compounds 1 , 2 , and 9 into the huperzine A binding site of AChE.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·
IF 15
Xie Wenjie,Xu Qianfeng,Yao YueXian,Chen Muqi,Zou Fen,Lin Jundong,Zhong Guowei,Zhou Haoran,Xu Jiaquan,Luo Pu,Liang Zhenguo,Du Qiuling,Li Jibiao,Zhang Jinxiang,Lu Puyang,Zhang Yixun,He Huichan,Zhuo Yangjia,Zhong Weide
Prostate cancer (PCa) is the most prevalent malignant tumor in the male genitourinary system. Carbon monoxide (CO) gas therapy and chemodynamic therapy (CDT) have emerged as alternative treatments for cancer. However, the therapeutic efficacy of both approaches is constrained by the insufficient endogenous hydrogen peroxide (H 2 O 2 ) supply and the overexpression of protective glutathione (GSH) in the tumor microenvironment (TME). Herein, we developed a biomimetic cascaded nanoreactor (DCM@MCuH@MnCO) for synergistically combining CO-mediated gas therapy with CDT driven by depletion of GSH and self-supply of H 2 O 2 in the tumor. DCM@MCuH@MnCO was constructed by integrating a copper-catechol coordinated network (CuH) and H 2 O 2 -sensitive manganese carbonyl (MnCO) within the mesoporous carbon hollow spheres (MCHS), and further modifying the surface with tumor cell membranes (DCM) to enhance its tumor-targeting ability. The CuH in the nanoreactor can disassemble, triggered by continuous consumption of GSH, releasing pro-oxidative catechol ligands to generate H 2 O 2 for TME remodeling. Subsequently, MnCO decomposes on demand to release CO in situ while copper ions (Cu + ) convert H 2 O 2 into reactive oxygen species (ROS) via a Fenton-like reaction. These events cooperatively cause mitochondrial damage and induce a lethal ROS storm within cells, ultimately effectively killing tumor cells both in vitro and in vivo. This study demonstrates significant prospects for CO therapy and CDT, offering a novel and promising strategy for efficient PCa treatment. Graphical
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Science China-Materials
·
2026
·
IF 7.7
Cao Shichun,Guo Yifan,Ni Yufeng,Huang Hongfei,Wang Yuepeng,Su Jilin,Jia Yujie,Qian Bo,Guan Qingbao,You Zhengwei
The three-dimensional (3D) printing of thermoset liquid metal (LM)-based flexible electronics holds considerable promise for various applications. However, the high surface tension and density of LM tend to cause sedimentation during the printing process, presenting significant challenges for 3D printing. Additionally, current 3D-printed thermoset LM-based flexible composite materials are not recyclable, resulting in resource wastage. In this work, a disulfide bond-based dynamically crosslinked polyurethane (SPU) is introduced as a solvent-free ink for fabricating an elastomer-liquid metal composite (SPU-LM). The crosslinking units in the SPU-LM ink are designed as Diels-Alder (DA) adducts, which undergo reversible dissociation upon heating, transitioning into a molten state. The multifunctional disulfide bonds facilitate interactions between the LM micro-nanoparticles and the polymer network, improving the dispersion of the LM and preventing sedimentation. These disulfide bonds also enhance the mechanical properties and self-healing capability of the material. The printed SPU-LM wires exhibit excellent stretchability, mechanical durability, near-infrared light-induced self-healing, and conductivity. Notably, these wires retain their mechanical and electrical properties even after multiple printing cycles. The results demonstrate the potential for sustainable printing of LM-based flexible electronics. Furthermore, the LM can be easily recycled, contributing to sustainability in flexible electronics fabrication.
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METABOLIC BRAIN DISEASE
·
2026
·Vol. 41
·期 1
·226
·
IF 3.8
Ren Guoqing,Yang Wenbo,Wang Jiajia,Zhang Jinping,Gao HuiFang,Gao Xia,Chen Xialin,Wang Tuanjie,Cao Liang,Wang Zhenzhong,Xiao Wei
The gut-brain axis represents an emerging therapeutic target. This study seeks to investigate the regulatory effects of LXTL on the brain-gut axis and to elucidate its mechanism of action during the ischemic stroke recovery period. This study investigated the effects of LXTL on a middle cerebral artery occlusion and reperfusion model. The assessment of recovery effects was conducted through the evaluation of brain injury scores, Y-maze performance, open field tests. Furthermore, comprehensive analyses were conducted on the gut microbiota, damaged brain tissue, cecal contents, and brain samples using metagenomic sequencing, transcriptomic sequencing, and non-targeted metabolomics. And the selected targets were verified. Integrated analyses were conducted by constructing correlation networks and performing joint pathway enrichment to elucidate the connections between microbial alterations, metabolite changes, and gene expression modifications. LXTL improved cognitive and exploratory behaviors, reduced brain damage and neuron loss, and enhanced gut health by increasing beneficial bacteria like Eubacterium and Kurthia. It boosted SCFAs, neuroactive metabolites, and altered nucleotide metabolism. LXTL is linked to genes involved in neurorepair and immune homeostasis, correlates with microglial polarization, and is associated with genes such as Ngfr, Card9, Chat, Nkg7, and Gch1 within the pertinent pathways. The integrated network linked LXTL-enhanced microbiota, gut and brain metabolites, and repair genes, facilitating recovery through a neurorepair-immune-metabolic triad. LXTL aids ischemic stroke recovery by altering gut microbiota and gut-brain metabolite profiles, while regulating brain gene networks related to neuroplasticity, inflammation, and metabolism. This demonstrates LXTL’s multi-target effects through the gut-brain axis, underscoring the potential of herbal medicine in stroke rehabilitation. Graphical
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2026
·
IF 3.3
Ma Yong-hu,Ma Fu,Zhao Tiao-bin,Gao Zi-yi,La Ying-hong,Zhao Hong-jian
Limited interfacial charge separation efficiency and insufficient redox capacity severely hinder the practical application of conventional Bi 2 WO 6 -based photocatalysts. To address these issues, a novel Bi(C 2 O 4 )OH/Bi 2 WO 6 heterojunction was constructed via a facile room-temperature one-pot liquid-phase deposition method. Multiple characterizations confirm the intimate interfacial contact between Bi(C 2 O 4 )OH nanorods and uniformly anchored Bi 2 WO 6 nanoparticles, accompanied by the introduction of abundant oxygen vacancies. On the basis of radical trapping experiments, EPR measurements, and electrochemical tests, an S-scheme charge-transfer model is proposed. This model retains the strong redox potentials of both semiconductors, while oxygen vacancies are speculated to act as efficient interfacial charge-trapping and transfer sites to synergistically promote the migration and separation of photogenerated carriers. The optimized 7%Bi(C 2 O 4 )OH/Bi 2 WO 6 composite achieves an RhB removal efficiency of 97.6% within 60 min for 50 mL of RhB solution (30 mg·L -1 ) under 5-W LED lamp irradiation. Radical trapping and EPR tests confirm that ·O 2 - , h⁺, and ·OH are the dominant active species. This work presents a facile and efficient room-temperature fabrication strategy for Bi-based heterojunctions and systematically elucidates the synergistic promotion mechanism between oxygen vacancies and the proposed S-scheme charge-transfer pathway, offering new insights for designing high-performance photocatalysts for organic wastewater remediation.
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npj Science of Food
·
2026
·
IF 9.7
Yan Jiannan,Zhang Tongtong,Liu Pan,Jin Jiayi,Li Zhuo,Xu Xiuying,Zhang Hao,Zhao Chengbin,Liu Jingsheng
Ultrasound enhancement technology (120–360 W) was used to treat zein and soybean lipophilic protein (LP) complex nanoparticles, which were then used to stabilize Pickering emulsion gels. Appropriate ultrasound facilitated zein-LP interactions, leading to a more ordered, flexible, and stable complex protein structure. The particle size was reduced to 105.23 nm, and the contact angle was close to 90°, which promoted interfacial affinity. Ultrasound treatment at 240 W formed a denser microstructure of zein-LP complex emulsion gels, which also led to a more uniform distribution of emulsified oil droplets. The gel strength and water holding capacity were increased to 28.2 g and 87.3%, respectively. Meanwhile, the rheological properties, physical stability, and thermal stability of the emulsion gels were improved. In addition, ultrasound-enhanced zein-LP complex emulsion gels could be applied as ideal edible inks in 3D printing of self-supporting products with high resolution. Therefore, controlling ultrasonic power improves the stability of emulsion gels by promoting structural modifications and intermolecular interactions of complex protein nanoparticles. These findings provide a theoretical basis and technical guidance for understanding the stability mechanism of ultrasound-enhanced zein-LP complex emulsion gels, as well as for the construction and application of highly stable functional emulsion gels.
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npj Parkinsons Disease
·
2026
·
IF 8.5
Li Zhihong,Cui Peijing,Zhang Lei,Huang Xiaojun,Zhou Yifan,Xu Siqi,Mao Yanfei,Wang Yanping,Liu Li,Zhang Yu
Parkinson’s disease (PD) lacks reliable non-invasive early diagnostic biomarkers with strong discriminative capacity, representing a major unmet clinical demand. We developed a dansylation workflow coupled to trapped ion mobility spectrometry–quadrupole time-of-flight mass spectrometry (TIMS-Q-TOF-MS) for targeted profiling of the urinary amine–phenol submetabolome. Urine samples from 21 patients with PD, 21 patients with Alzheimer’s disease (AD), and 21 healthy controls (HCs) were analyzed, yielding annotations for 196 metabolites: 143 amine-containing species, 49 phenolic metabolites, and 4 dual-function metabolites carrying both amine and phenolic moieties. Partial least squares discriminant analysis (PLS-DA) confirmed the stability of our analytical pipeline. Screening with thresholds VIP > 1.2 and p < 0.05 identified 47 differentially abundant metabolites linked to PD, enriched in cysteine/methionine metabolism, phenylalanine–tyrosine–tryptophan biosynthesis, pyrimidine metabolism, and tryptophan metabolism. PD was characterized by elevated dopamine 3-O-sulfate—indicative of accelerated peripheral dopamine inactivation—and reduced tryptophan and its hydroxylated derivatives. Receiver operating characteristic (ROC) analysis validated a three-metabolite signature (4-amino-1-piperidinecarboxylic acid, dopamine 3-O-sulfate, methylacetylenic putrescine) with an AUC of 0.923 (95% CI: 0.828–0.986) to discriminate PD from HCs. This panel captures PD-specific neurotransmitter metabolic dysregulation and achieves robust patient stratification. This study’s advantages lie in targeted quantification of pathophysiologically relevant amine–phenol metabolites, convenient non-invasive urinary sampling, and enhanced detection sensitivity via 4D metabolomics. Collectively, our results establish a novel urine-based diagnostic strategy for PD and delineate divergent metabolic signatures that differentiate PD from AD and other prevalent neurodegenerative disorders.
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CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·
IF 3.1
Wang Yunlong,Sun Yue,Wang Le,Zhang Hao,Hu Jiakang,Li Yongxin
Phenolic compounds, classified as persistent organic pollutants, pose a significant threat to both human health and environmental safety. Therefore, the efficient removal of phenolic substances from water is of paramount importance. Laccase, a multicopper oxidase, is commonly utilized for the efficient removal of phenolic contaminants from water due to its highly effective catalytic activity towards phenolic compounds. However, natural laccase exhibits certain limitations that impede its practical implementation in industrial settings, including a restricted pH activity range, diminished enzymatic efficacy at elevated temperatures, and substantial cost implications. In this work, we prepared a nanozyme (MIZ-Cu, MIZ: 2-methylimidazole) with laccase-like activity by coordinating 2-methylimidazole and copper. This nanozyme overcomes the deactivation issues observed in natural laccase under high temperature and alkaline conditions. The catalytic activity of the MIZ-Cu towards phenolic compounds surpasses that of natural laccase across a wide range of temperature and pH conditions. Under pH=9, 80 °C, and 500 mmol/L NaCl conditions, the removal rate of four phenols (catechol, hydroquinone, resorcinol, and phloroglucinol) by MIZ-Cu was much higher than that of natural laccase. The results also demonstrate exceptional removal rates in natural aquatic environments, thereby presenting a promising approach for the treatment of phenol-containing wastewater originating from industrial facilities.
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APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2023
·Vol. 129
·期 12
·1-8
·
IF 2.7
Gao Ningning,Wang Qing,Tang Jun,Lv Xiaoyi,Li Hongmei,Yue Xiaxia,Zhong Furu,Fu Jihong,Wang Tao
Gold nanoparticles/785 porous silicon photonic crystals (Au NPs/785 PSi PhCs) were used as substrates in combination with a low-concentration serum surface-enhanced Raman spectroscopy (SERS) detection scheme to obtain spectral signals from healthy individuals and cervical cancer patients. The best principal component scores were selected by principal component analysis (PCA) combined with linear discriminant analysis (LDA) and support vector machine (SVM) to analyze the spectral differences distinguishing healthy and cervical cancer patients. The accuracy of the two models was 97.9% and 96.9%, respectively. SERS technique based on Au NPs/785 PSi PhCs has great potential to improve the screening of cervical cancer.
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BMC CANCER
·
2023
·Vol. 23
·期 1
·1-13
·
IF 3.8
Liu Bin,Zheng Xin,Li Jiajun,Yao Peng,Guo Peng,Liu Wei,Zhao Gaoping
Background Colorectal cancer is a common malignant tumour. Invasive growth and distant metastasis are the main characteristics of its malignant biological behaviour, and they are also the primary factors leading to death in colon cancer patients. Atovaquone is an antimalarial drug, and its anticancer effect has recently been demonstrated in several cancer models in vitro and in vivo, but it has not been examined in the treatment of colorectal cancer. Methods To elucidate the effect of atovaquone on colorectal cancer. We used RNA transcriptome sequencing, RT‒PCR and Western blot experiments to examine the expression of NF-κB (p-P65), EMT-related proteins and related inflammatory factors (IL1B, IL6, CCL20, CCL2, CXCL8, CXCL6, IL6ST, FAS, IL10 and IL1A). The effect of atovaquone on colorectal cancer metastasis was validated using an animal model of lung metastases. We further used transcriptome sequencing, the GCBI bioinformatics database and the STRING database to predict relevant target proteins. Furthermore, pathological sections were collected from relevant cases for immunohistochemical verification. Results This study showed that atovaquone could inhibit colorectal cancer metastasis and invasion in vivo and in vitro, inhibit the expression of E-cadherin protein, and promote the protein expression of N-cadherin, vimentin, ZEB1, Snail and Slug. Atovaquone could inhibit EMT by inhibiting NF-κB (p-P65) and related inflammatory factors. Further bioinformatics analysis and verification showed that PDGFRβ was one of the targets of atovaquone. Conclusion In summary, atovaquone can inhibit the expression of NF-κB (p-P65) and related inflammatory factors by inhibiting the protein expression of p-PDGFRβ, thereby inhibiting colorectal cancer metastasis. Atovaquone may be a promising drug for the treatment of colorectal cancer metastasis.
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