JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2018
·
IF 1.911
Feng Su,Yuandou Wang,Xue Liu,Xin Shen,Xingjian Zhang,Quansheng Xing,Lihong Wang,Yangsheng Chen
Carboxymethyl chitosan-graft-polylactide (CMCS-PLA) and carboxymethyl chitosan (CMCS) hydrogels were prepared by using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS) as crosslinking agent and catalyst at room temperature. The biocompatibility of the hydrogels was evaluated with the aim of assessing their potential as drug carrier. Various aspects of biocompatibility were considered, including MTT assay, agar diffusion test, release of lactate dehydrogenase (LDH), hemolytic test, plasma recalcification time (PRT), and dynamic clotting time. MTT assay showed that the cytotoxicity level of both hydrogels to L-929 cells was 0 or 1. The LDH release of CMCS and CMCS-PLA was 26 and 29%, respectively, which is slightly higher than that of the negative control (21%) and much lower than that of the negative control (87%). The hemolysis ratio of CMCS and CMCS-PLA was 1.4 and 1.7%, respectively, suggesting outstanding anti-hemolysis properties of both materials. The PRT value of CMCS and CMCS-PLA was higher by 77 and 99% than the value of the positive control. All the results revealed that the hydrogels present good cytocompatibility and hemocompatibility in vitro. In vivo degradation and tissue compatibility were evaluated by subcutaneous injection in the dorsal area of rats. CMCS and CMCS-PLA hydrogels were completely degraded and the inflammatory response also completely disappeared around hydrogels after 19 days in vivo. It is thus concluded that hydrogels formed of CMCS and CMCS-PLA with outstanding biocompatibility are promising as potential drug carrier.
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高影响因子
Journal of Cleaner Production
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2018
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IF 5.651
Lingxiao Liu,Haiyan Liang,Jing Zhang,Pengfei Zhang,Quan Xu,Qiming Lu,Chaoqun Zhang
The development of sustainable and transient materials is not only significant importance for environmental concern and human health, but also meets the highly demand for specific application in biomedical, military and intelligence fields. In this study, polymer blends of poly(vinyl alcohol) and chitosan were used to prepare sustainable and degradable polymer films which are promising to be applied as substrates for transient bioelectronics. The effect of the material composition and chemical structures on the mechanical, thermo-physical and thermal stability was studied and characterized by tensile testing , dynamic mechanical analysis , thermogravimetric analysis , and Fourier-transform infrared spectroscopy, respectively. In addition, the transiency rate of the composites as a function of temperature and composition was investigated and discussed. It is found that the transience rate of the composites can be tailored by controlling the composition and chemical structures. Addition of chitosan tends to decrease the time constant. In addition, the temperature also strongly affects the transience behavior of the polymer blends . The tailored performance and programmable degradation of these novel polymer composites provide a sustainable platform for widely application in transient biomedical and electronic devices.
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高影响因子
ANALYTICAL CHEMISTRY
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2018
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IF 6.042
Gen Liu,Cheng Ma,Bao-Kang Jin,Zixuan Chen,Jun-Jie Zhu
Single-cell imaging is essential for elucidating the biological mechanism of cell function because it accurately reveals the heterogeneity among cells. The electrochemiluminescence (ECL) microscopy technique has been considered a powerful tool to study cells because of its high throughput and zero cellular background light. However, since cells are immobilized on the electrode surface, the steric hindrance and the insulation from the cells make it difficult to obtain a luminous cell ECL image. To solve this problem, direct ECL imaging of a single cell was investigated and achieved on chitosan and nano-TiO2 modified fluoride-doped tin oxide conductive glass (FTO/TiO2/CS). The permeable chitosan film is not only favorable for cell immobilization but also increases the space between the bottom of cells and the electrode; thus, more ECL reagent can exist below the cells compared with the cells on a bare electrode, which guarantees the high sensitivity of quantitative analysis. The modification of nano-TiO2 strengthens the ECL visual signal in luminol solution and effectively improves the signal-to-noise ratio. The light intensity is correlated with the H2O2 concentration on FTO/TiO2/CS, which can be applied to analyze the H2O2 released from cells at the single-cell level. As far as we know, this is the first work to achieve cell ECL imaging without the steric hindrance effect of the cell, and it expands the applications of a modified electrode in visualization study.
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高影响因子
FIBERS AND POLYMERS
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2018
·Vol. 19
·期 2
·340-349
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IF 1.353
Wang Yixi,Wang Hao,Peng Huili,Wang Zhicun,Wu Jianning,Liu Zhiyong
A novel eco-friendly porous adsorbent of cellulose (CE)/chitosan (CS) aerogel was prepared through sol-gel process and freeze-drying to remove Congo Red (CR). A series of aerogels were prepared by adjusting the mass ratios of CE and CS. Composite aerogels were characterized by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). SEM images showed that it was possible to change the structure of the aerogel by adjusting the amount of chitosan. The effects of dosage of chitosan, initial pH, temperature, adsorbent dosage, contact time, and initial dye concentration on adsorption capacities for CR were studied in detail. Batch adsorption studies showed that aerogel exhibited maximum removal efficiency to CR at a composite ratio of 1:3 and dosage of 2.5 g/ l . CE/CS aerogel had excellent adsorption capacities for CR at a pH range of 3-11, which indicated stability of the aerogel in both acidic and alkaline conditions. CR adsorption on the composite aerogel fitted pseudo-second-order kinetics and Langmuir isotherm. The Langmuir isotherm model revealed that the maximum theoretical adsorption capacity of this material for CR was 381.7 mg/g at pH 7.0 at 303 K for 24 h. The adsorption mechanism included electrostatic and chemical interactions. The results indicated that the adsorption capacity of CE/CS aerogels was higher than the other chitosan composites adsorbents.
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高影响因子
Journal of Cleaner Production
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2018
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IF 5.651
Shuting Zhuang,Rong Cheng,Mi Kang,Jianlong Wang
Uranium is used to make nuclear fuels for power generation . Uranium-containing wastewater is produced from fuel fabrication, fuel reprocessing , which resulted in the soil and water pollution . In this study, a cleaner and environmental friendly adsorption material, chitosan, was used to remove uranium from aqueous solution. Magnetic amidoxime functionalized chitosan beads were synthesized, characterized and applied for adsorption of uranium. The adsorption capacity of 117.65 mg/g was achieved at pH = 6, mainly due to coordination ability of amidoxime groups. With an Ms of 21.7 emu/g, the magnetic adsorbents could be fast separated from aqueous solution by the magnetic field. Additionally, the results showed that the adsorption process could be well described by the pseudo-second order kinetic (R 2 = 0.9842) and Langmuir models (R 2 = 0.9995). Finally, an adsorption mechanism was tentatively proposed to explain the adsorption process.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
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IF 3.909
Weixiang Liu,Yukun Qin,Song Liu,Ronge Xing,Huahua Yu,Xiaolin Chen,Kecheng Li,Pengcheng Li
A novel type of water soluble chitosan derivatives (TQCSPX) were synthesized including 3-aminopyridine (TQCSP1) and 3-Amino-4-methylpyridine (TQCSP2). The theoretical structures of TQCSPX were calculated by Gaussian 09 and confirmed by FT-IR, 1 H NMR, 13 C NMR, elemental analysis and XRD. The antifungal properties of TQCSPX against Phytophthora capsici ( P. capsici ), Rhizoctonia solani ( R. solani ), Fusarium oxysporum ( F. oxysporum ) and Fusarium solani ( F. solani ) were evaluated at concentrations ranging from 0.2 mg/mL to 0.8 mg/mL. Antifungal results indicated that the derivatives have significantly enhanced antifungal activity after quaternized compared with the original chitosan (CS). Moreover, TQCSP1 inhibited the growth of P. capsici with inhibitory indices of 91.94% at 0.8 mg/mL. The experimental results demonstrated that the increasing number of the positive charge would improve the antifungal efficiency of chitosan, which may provide a novel direction for the development of fungicides.
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高影响因子
Materials
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2018
·Vol. 11
·期 10
·2009
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IF 2.467
Moxi Wang,Li Feng,Xiaowei Fan,Dongmei Li,Wenqi Qu,Shuxian Jiang,Shaoxiu Li
In this study, a series of chitosan-based quaternary ammonium graft flocculants, namely chitosan-graft-poly(acrylamide and methacryloyl ethyl trimethyl ammonium chloride) [CTS-g-P(AM-DMC)], was successfully synthesized by plasma initiation, and the as-prepared [CTS-g-P(AM-DMC)] had both flocculation and sterilization functions. Various characterization techniques were used to study the structure and physicochemical properties of the chitosan-based flocculants.1H nuclear magnetic resonance spectroscopy (1H NMR), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction spectroscopy (XRD), and thermogravimetric analysis/differential scanning calorimetry (TG/DSC) confirmed the successful synthesis of CTS-g-P(AM-DMC). Scanning electron microscopy (SEM) analysis exhibited that CTS-g-P(AM-DMC) contained a smooth convex and porous structure with an enormous surface area. CTS-g-P(AM-DMC) was then used to flocculate the simulated wastewater of the kaolin suspension and theSalmonellasuspension. Besides external factors, such as the dosage of flocculant and pH, the effect of the internal factor graft ratio was also evaluated. The experimental results showed that CTS-g-P(AM-DMC) also revealed a strong sterilization effect, aside from the excellent flocculation effect. Moreover, the sterilization mechanism was investigated through a series of conductivity measurements and the analysis of fluorescence-based cell live/dead tests. The results indicated that CTS-g-P(AM-DMC) destroyed the cell membrane ofSalmonellathrough its grafted quaternary ammonium salt, thereby exhibiting sterilization property.Keywords:chitosan-based flocculant;bifunctional;turbidity removal;sterilization;plasma initiation
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高影响因子
MOLECULES
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2018
·Vol. 23
·期 10
·2611
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IF 3.098
Xionglin Chen,Xiaoming Cao,He Jiang,Xiangxin Che,Xiaoyuan Xu,Baicheng Ma,Jie Zhang,Tao Huang
Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson’s trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds.Keywords:SIKVAV;chitosan hydrogel;skin wound healing;angiogenesis;growth factor
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高影响因子
Nanomaterials
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2018
·Vol. 8
·期 5
·326
·
IF 3.504
Wu Wen,Biao Han,Feng Yan,Liang Ding,Bojie Li,Liansheng Wang,Lei Zhu
We describe here the preparation of copper nanoparticles stabilized on a chitosan/poly (vinyl alcohol) composite film. This material could catalyze the borylation of α,β-unsaturated acceptors in aqueous media under mild conditions. The corresponding organoboron compounds as well as their converted β-hydroxyl products were all obtained in good to excellent yields. It is noteworthy that this catalyst of copper nanoparticles can be easily recycled eight times and remained catalytically reactive. This newly developed methodology provides an efficient and sustainable pathway for the synthesis of organoboron compounds and application of copper nanoparticles.Keywords:chitosan composite film;copper nanoparticles;organoboron compounds;easily recyclable
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高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Pan Zhang,Nan Zhang,Qiang Wang,Ping Wang,Jiugang Yuan,Jinsong Shen,Xuerong Fan
Chitosan, a natural biopolymer, is used as a multifunctional agent for modification of wool either through chemical crosslinking or physical coating. For the first time, wool fabric has been modified with chitosan through disulfide bond breaking and reforming reactions. The chitosan was thiolated and then grafted onto the reduced wool fibers through disulfide bonds. In order to understand the mechanism of the grafting of thiolated chitosan onto wool, glutathione was used as a model compound for wool in the research. The structures of thiolated chitosan reacted with glutathione and wool fabrics grafted with thiolated chitosan were investigated by FTIR, 13 CNMR, XPS, XRD, SEM. The dyeability, shrink-resistance and biocompatibility were also tested. The results suggested that glutathione reacted with thiolated chitosan and formed disulfide bond. The thiolated chitosan-grafted wool fabric had good shrink-resistance and dyeability. Hydrophilicity and antibacterial properties were also improved compared with untreated wool fabric. The results provide a novel approach for modification of wool through fiber-intrinsic groups like disulfide bonds.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Qian Wang,Jiayi Wang,Wu Ding,Dequan Zhang,Kevin Reed,Boce Zhang
Preservatives in processed meat raise significant concerns associated with bowel cancer and diabetes, and implicate various public health issues. This introduces the need for safer preservatives to uphold public health standards. However, developing safer alternatives to these preservatives poses a significant challenge to food industry. A potential solution to this issue is the use of loaded nanoparticles as preservative agents. This study investigated antimicrobial and antioxidant effects of sorbic acid-loaded chitosan/tripolyphosphate nanoparticles (SAN) in Chinese Sausage. SAN were prepared through ionic gelation, followed by natural air-drying for 20 days. After preparation, the antimicrobial and antioxidant activities of various treatment groups were analyzed intermittently during storage at room temperature. SAN-treated samples had significantly lower levels of surviving bacteria, molds, and yeasts than the blank control ( p < 0.05) over the entire 72 days of storage. Additionally the SAN-treated samples also had lower levels of surviving bacteria than the chitosan/tripolyphosphate samples after 31–56 days of storage ( p < 0.05). The thiobarbituric acid value and pH of the SAN-treated samples were also significantly lower than the blank control ( p < 0.05). These results indicated that SAN could be a good intervention strategy to retard lipid oxidation and inhibit microbial growth in Chinese Sausage.
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高影响因子
RENEWABLE ENERGY
·
2019
·
IF 5.439
Nisakorn Saengprachum,Dongren Cai,Mantian Li,Ling Li,Xiaocheng Lin,Ting Qiu
Via the crosslinking of chitosan and sulfosuccinic acid (SSA), the acidic chitosan membrane (ACM) was prepared for biodiesel production from esterification of oleic acid and methanol. ATR-FTIR, TGA, XRD, SEM and EDX were applied to characterize the prepared ACM. The acidic site density and swelling capacity of the prepared ACM were investigated. The ACM possessed the 4.62 mmol/g of acidic site density, which was just a little less than 4.76 mmol/g of Amberlyst-15, meanwhile, ACM was proven to be much higher than Amberlyst-15 in swelling capacity. Under the same reaction, the 98.76% of conversion can be obtained while the Amberlyst-15 only reached 44.30%. The optimization of process variables was conducted by the combination of single factor experiment and Box-Behnken design (BBD) response surface methodology . The catalytic activity of ACM in different esterification of fatty acids and alcohols was also investigated. And the catalytic mechanism of esterification catalyzed by ACM was clarified. Based on the catalytic mechanism, the kinetic model was established to describe the esterification and obtain relevant kinetic parameters ( E a + , E a − , A + and A - ), meanwhile, the kinetic results were compared to the same reaction with different catalysts in detail. Besides, the reusability of the prepared ACM was studied.
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