Journal of Water Process Engineering
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2026
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IF 6.6
Xiang Luo,Hongwei Chen,Hongjian Shen,Ling Zhang,Jinbo Chen,Shuai Liu,Bo Gao,Honglei Zhang,Yawei Gu
Zero-valent iron (ZVI) technology is regarded as one of the most efficient approaches for remediating groundwater contaminated with chlorinated hydrocarbons (CHCs). Despite its highly reactive nature, the corrosion of ZVI in aqueous solution readily results in surface passivation, which diminishes its reactivity. ZVI doped with heteroatoms such as carbon (C), nitrogen (N), and sulfur (S) has displayed excellent performance in trichloroethylene (TCE) degradation. Nevertheless, the effects of these heteroatoms on ZVI materials, along with their various degradation mechanisms, remains ambiguous. In this study, C-mZVI bm , N-mZVI bm , and S-mZVI bm were prepared, respectively, via a mechanochemical method and utilized for TCE degradation. The dechlorination reaction facilitated by each material was thoroughly investigated both theoretically and experimentally, focusing on TCE adsorption affinity, the dechlorination process, hydrogen evolution reaction (HER), electron efficiency, and lifetime. Among the three materials, S-mZVI bm exhibited 100% TCE removal in less than 24 h., as well as improved electron efficiency and lifetime, primarily attributed to its high TCE adsorption energy and the suppression of HER. These integrated theoretical and experimental results indicate that S-mZVI bm nanoparticles are a promising and innovative material for in situ groundwater TCE remediation.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2026
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IF 5.3
Huan Liu,Liying Liang,Minggang Wang,Qinfeng Huang,Yu Pan,Xiaojie Wei,Wenchao Zhang
The naturally occurring pentacyclic triterpenoid compounds ursolic acid (UA) and oleanolic acid (OA) are commonly found together in various fruits and find broad applications in pharmaceuticals, cosmetics, food and agricultural fields. In this study, a sensitive and practical icELISA method was developed based on a monoclonal antibody (mAb) YC23-101, with a median inhibitory concentration (IC 50 ) of 15.56 ng/mL and a linear range of 5.26 to 54.1 ng/mL. The limits of detection (LOD) ranges from 0.98-5.11 ng/mL in fruit matrices and the recoveries were 94.03-112.21% with coefficients of variation below 5.73%. Cross-reactivity analysis showed that mAb YC23-101 could also specifically recognize OA with similar sensitivity to UA, indicating feasibility for the simultaneously quantification of UA and OA in fruits. The developed icELISA was applied to several fruits, and the results showed good correlation with that of HPLC. Molecular docking analysis predicted equal binding energy of UA and OA to the mAb with hydrophobic interactions playing a predominant role. All these findings demonstrated that this icELISA is reliable, sensitive, and suitable for the high-throughput quantification of total UA and OA content in fruits composition analysis.
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Nature Communications
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2026
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IF 18.1
Cao Jie,Yang Nailin,Gong Fei,Wang Xi,Qiao Xinyi,Pan Xiaoxiao,Du Qialu,Cai Yichi,Wu Jie,Huang Yechen,Wang Yichen,Han Zhihui,Cheng Liang
Gouty arthritis faces multiple challenges, such as recalcitrance to cure and recurrent flares, owing to its intricate pathophysiological microenvironment. Recent studies have focused on urate reduction, pain relief, or anti-inflammation effects alone, which often fail to provide satisfactory results. To address these challenges, an implantable manganese platinum microscale galvanic cell (Mn@Pt micro-GC) is developed. This system utilizes the urate oxidase-like activity of platinum to degrade deposited monosodium urate crystals, while the Mn 2+ and hydrogen gas work synergistically to effectively reversing the pro-inflammatory environment. In addition to decreasing the production of pain signals associated with gout, Mn@Pt micro-GC effectively inhibits pain signal transmission along the CXCL5-neuronal CXCR2-TRPA1 axis through continuous electrical stimulation. This multimodal treatment approach emphasizes the strong relationship between bone and nerve in gout, taking into account the importance of reducing uric acid, anti-inflammation, and pain relief in gout therapy, which has led to improved treatment results.
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High Temperature Corrosion of Materials
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2026
·Vol. 103
·期 2
·19
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IF 2.2
Liu Qinghua,Han Lu,Wu Mingzhen,Wang Guoao,Liu Jiazhan
The synergistic oxidative and tellurium-induced (Te-induced) corrosion of the fast-reactor candidate 15–15Ti austenitic stainless steel under high oxygen potentials was investigated in this study. Specimens were exposed at 900 K in both Te-free and Te-containing environments, with the oxygen potentials controlled using Mo/MoO 2 and Ni/NiO redox buffers. Under Te-free conditions, a duplex oxide scale formed on 15–15Ti, comprising an Fe-rich outer oxide layer and a Cr-enriched inner oxide layer. In contrast, in the presence of Te, a markedly thicker multilayer reaction scale developed, consisting of four distinct layers arranged from the surface inward: (i) a Mn–O outer oxide layer; (ii) an Fe–Ni–Te metallic telluride layer; (iii) a Cr-enriched outer telluride reaction layer with Cr 3 Te 4 as the dominant phase; and (iv) an inner Te/Cr-enriched reaction layer, in which a phase consistent with Cr 2 Te 4 O 11 was identified. These findings indicate that, under high oxygen potentials, corrosion of 15–15Ti is governed by the coupled action of Te and oxidation. The introduction of Te more strongly promotes outward diffusion of metal cations (especially Fe) and suppresses the formation of a protective Cr-rich oxide layer, thereby markedly accelerating scale thickening and stabilizing the multilayer architecture.
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ANALYTICA CHIMICA ACTA
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2026
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IF 6.1
Baizong Fang,Wei Guo,Zhenyu Liu,Huakui Huang,Xiaohong Zhong
Background The detection and imaging of intracellular biomarkers can provide important information for the early diagnosis of cancer. Leveraging the inherent advantages of DNA such as biocompatibility, ease of synthesis, programmability, and sequence-specific recognition, DNA-based probes have been widely employed for biomarker analysis. Currently, a variety of DNA probes combined with signal amplification methods have been widely used to visualize biomarkers in living cells. However, conventional single-locked DNA probes cannot ensured the accurate diagnosis. Furthermore, the lack of efficient cascade signal amplification strategies makes it challenging to detect and image low-abundance biomarkers. Results In this work, we report a dual-locked, self-feedback catalytic hairpin assembly (CHA)-DNAzyme cascade amplification platform, termed hMNS@SCD. This platform utilizes MnO 2 nanoflowers as nucleic acid carriers and DNAzyme cofactor supplement, employing apurinic/apyrimidinic endonuclease 1 (APE1) and miR-21 as two molecular keys. When the APE1 and miR-21 existence, hairpin probe carry out the CHA cycle, leading to the assembly of a Y-shaped DNAzyme complex. When Mn 2+ is present, the Y-shaped DNAzyme can undergo a hybridization-cleavage-release cycle on the MB, leading to a gradual restoration of Cy5 fluorescence. In addition, the released target analogue from MB can trigger a new CHA cycle, generating more Y-shaped DNAzyme complexs and cleaving more MB, further amplifying the fluorescence signal. Through the self-feedback CHA-DNAzyme cascade amplification mechanism, hMNS@SCD effectively enhances the detection sensitivity, with a detection limit for miR-21 reaching 34.6 fM. Experimental data show that hMNS@SCD has high sensitivity, selectivity, and biocompatibility. Significance we constructed a dual-locked, self-feedback CHA-DNAzyme cascade nanomachine, which enables tumor-specific molecular imaging and effectively distinguishes between normal cells and cancer cells. In addition, due to the programmability of nucleic acid probes, this strategy can be readily adapted for detection and imaging of other low-abundance biomarkers, offering a promising way forward for tumor-specific molecular imaging and early cancer diagnosis.
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Biointerphases
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2022
·Vol. 17
·期 6
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IF 1.916
Wang Li,Cui Baocheng,Qiu Keyuan,Huang Jiao,Liang Changhai
Nitrile hydratase (NHase, EC 4.2.1.84) is an excellent biocatalyst that catalyzes the hydration of nitrile substances to their corresponding amides. Given its catalytic specificity and eco-friendliness, NHase has extensive applications in the chemical, pharmaceutical, and cosmetic industries. To improve the affinity between Rhodococcus erythropolis CCM2595-derived NHase ( Re NHase) and adiponitrile, this study used a semirational design to improve the efficiency of Re NHase in catalyzing the generation of 5-cyanopentanamide from adiponitrile. Enzyme kinetics analysis showed that Km of the mutant Re NHase B:G196Y was 3.265 mmol l −1 , which was lower than that of the wild-type NHase. The affinity of the mutant Re NHase B:G196Y to adiponitrile was increased by 36.35%, and the efficiency of the mutant Re NHase B:G196Y in catalyzing adiponitrile to 5-cyanopentamide was increased by 10.11%. The analysis of the enzyme-substrate interaction showed that the hydrogen bond length of the mutant Re NHase B:G196Y to adiponitrile was shortened by 0.59 Å, which enhanced the interaction between the mutant and adiponitrile and, thereby, increased the substrate affinity. Similarly, the structural analysis showed that the amino acid flexibility near the mutation site of Re NHase B:G196Y was increased, which enhanced the binding force between the enzyme and adiponitrile. Our work may provide a new theoretical basis for the modification of substrate affinity of NHase and increase the possibility of industrial applications of the enzyme.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2022
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IF 4.831
Yi He,Xiangjian Liao,Haonan Wu,Jialiang Huang,Yi Zhang,Yanyu Peng,Zhen Wang,Xin Cao,Caijun Wu,Xiaojun Luo
In this work, a simple and sensitive SERS-based miRNA biosensor was constructed based on porous MOFs nanoparticles and efficient subject-object recognition ability. MOFs as a container can package lots of signal probe neutral red (NR) for the advantages of three dimensional structure and porosity. The partially complementary duplex DNA can as a “lock” to lock up the hole for obtaining a weak Raman signal. In the present of miRNA, miRNA just like a “key” to open the duplex structure with the results of releasing NR. At this time, the released NR can be captured by SERS substrate [email protected] [7] for the subject-object recognition ability to produce a strong Raman signal which was positive correlation to target miRNA. By this way, the proposed SERS biosensor can achieve sensitively and selectively detect miRNA with a detection limit of 0.562 fM. This MOF-based SERS biosensor also be hopeful application for clinical diagnostics.
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Materials Today Nano
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2022
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IF 13.364
X. Cui,Y. Zhou,Y. Zheng,L. Cao,L. Gao,J. Duan,H. Yin,S. Ai
N 6 -methyladenosine (m 6 A) is the most abundant and dynamic reversible modification of mRNA in eukaryotes discovered in the mid-1970s, which is widely involved in various physiological processes. Fat mass and obesity-associated protein (FTO protein) is not only a demethylase for m 6 A, but also a carcinogenic factor for many human cancers. Therefore, it is very important to build a simple and sensitive platform for simultaneous detection of m 6 A and FTO protein. To achieve this aim, a novel photoelectrochemical biosensor was constructed with the antibody-free detection strategy of DTT mediated –SH functionalization of m 6 A triggered by FTO protein catalyzed m 6 A oxidation . To achieve high detection sensitivity, CdS/Bi 2 MoO 6 /MoSe 2 system with matched energy gap was employed as photoactive material. To achieve high detection selectivity , the –CH 3 of m 6 A in the captured RNA sequence (m 6 A RNA) on the electrode surface was oxidized by FTO protein to form hydroxymethyl, followed with the covalent reaction of DTT with hydroxymethyl, making the labeling of m 6 A with –SH. Finally, a CdS nanoparticle was modified on the electrode through the specific covalent reaction between –SH and Cd, achieving the improvement of the photocurrent with the matched energy band . Under the optimal experimental conditions, the biosensor showed linear range from 0.001 to 50 nM and 0.0005–500 μg/L with the detection limit of 0.37 pM and 0.034 ng/L (S/N = 3) for m 6 A RNA and FTO protein, respectively. The applicability of the developed method was assessed by investigating the effect of entacapone on FTO protein activity.
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FOOD HYDROCOLLOIDS
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2022
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IF 11.504
Lunjie Huang,Da-Wen Sun,Hongbin Pu,Cuiyun Zhang,Daorui Zhang
The growing concerns about microbial food safety and environmental contamination call for sustainable food active packaging materials and practices with high performance. This contribution proposed a biocatalytic spraying coating for fruit preservation using carboxymethyl cellulose nanofibers grafting enzyme-like metal-organic frameworks ( [email protected] ) with banana and mango as model fruits. The well-characterized biohybrid nanofibers were verified to simultaneously mimic good oxidase-like and apyrase-like activities, enabling effective enzyme-like sterilization against three typical foodborne pathogens, i.e. Escherichia coli O157: H7, Staphylococcus aureus , and methicillin-resistant Staphylococcus aureus , reaching killing rates as high as 90.46%. The biocidal •O 2 − generation and adenosine triphosphate depletion were the possible mechanisms causing the nonspecific bacteria death. Such antibacterial performance, as well as additional protective properties of [email protected] , facilitated the quality preservation during the 12-day storage of bananas and mangos by spray coating with the biomimetic [email protected] It is expected that this work walks out the first step of nanozyme toward active packaging engineering for the fields of food, agriculture, and biomedicine.
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DYES AND PIGMENTS
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2022
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IF 5.122
Xiaoqing Xiong,Ye Yuan,Gengwen Chen,Miao Li,Lihua Lyu,Hongjuan Zhao,Jun Yan,Yulan Qian,Junyang Zhu,Chengqi Jiao
The investigation on the construction strategy, relationship between the structure and photophysical properties of fluorescein analogs plays an important role in the rational design of specific fluorescent dyes. In this work, a fluorescein analog Fc-1 , containing a reactive aldehyde group was prepared using 2′, 7′-dichlorofluorescein (DCF) as the raw material and Vilsmeier–Haack reaction mechanism. Meanwhile, two more fluorescein analogs Fc-2 and Fc-3 were synthesized based on Fc-1 and the Knoevenagel reaction mechanism. The results of Fc-1 indicated that the yield of Fc-1 was higher than 95% after a step of the low-temperature hydrolysis (40 °C) from an intermediate imine salt DCF-3 . The fluorescence quantum yield ( Φ ) of Fc-1 was relatively high in polar protic solvents, even in H 2 O ( Φ = 0.25). Fc-1 had large Stokes shifts in different solvents (≧ 123 nm), which was indentified by the Gaussian calculation of Fc-1 . Meanwhile, Fc-1 was also used as a ratio and colorimetric fluorescent probe for H 2 S in vitro , and the dianion ( [Fc-1-2H] 2- ) mechanism for detecting H 2 S was proposed by nuclear magnetic resonance (NMR), pH titration, and Gaussian calculation. Additionally, Fc-1 can be used in leukocyte classification because of its relatively high Φ in water, cell permeability, and cytotoxicity. All the data of dyes Fc-2 and Fc-3 indicated that the derivatization of the carboxyl group for Fc-2 did not affect the photophysical properties of Fc-2 . Both of them had relatively large Stokes shifts and long emission wavelengths . The large Stokes shift of Fc-2 and Fc-3 was due to the distribution of electron clouds in the excited states of Fc-2 and Fc-3 . The results of pH titration proved that dyes Fc-2 and Fc-3 were sensitive to the changes in pH and exhibited their potentials as pH probes. Thus, the strategy of constructing fluorescent dyes based on Vilsmeier–Haack and Knoevenagel reactions and the relationship between structure and properties will be beneficial to developing more dyes with large Stokes shift.
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International Journal of Nanomedicine
·
2023
·
IF 8
Jingmou Yu,Liangliang Wang,Xin Xie,Wenjing Zhu,Zhineng Lei,Linghui Lv,Hongling Yu,Jing Xu,Jin Ren
Background Multifunctional stimuli-responsive nanoparticles with photothermal-chemotherapy provided a powerful tool for improving the accuracy and efficiency in the treatment of malignant tumors.Methods Herein, photosensitizer indocyanine green (ICG)-loaded amorphous calcium-carbonate (ICG@) nanoparticle was prepared by a gas diffusion reaction. Doxorubicin (DOX) and ICG@ were simultaneously encapsulated into poly(lactic-co-glycolic acid)-ss-chondroitin sulfate A (PSC) nanoparticles by a film hydration method. The obtained PSC/ICG@+DOX hybrid nanoparticles were characterized and evaluated by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), transmission electron microscopy (TEM), and differential scanning calorimetry (DSC). The cellular uptake and cytotoxicity of PSC/ICG@+DOX nanoparticles were analyzed by confocal laser scanning microscopy (CLSM) and MTT assay in 4T1 cells. In vivo antitumor activity of the nanoparticles was evaluated in 4T1-bearing Balb/c mice.Results PSC/ICG@+DOX nanoparticles were nearly spherical in shape by TEM observation, and the diameter was 407 nm determined by DLS. Owing to calcium carbonate and disulfide bond linked copolymer, PSC/ICG@+DOX nanoparticles exhibited pH and reduction-sensitive drug release. Further, PSC/ICG@+DOX nanoparticles showed an effective photothermal effect under near-infrared (NIR) laser irradiation, and improved cellular uptake and cytotoxicity in breast cancer 4T1 cells. Importantly, PSC/ICG@+DOX nanoparticles demonstrated the most effective suppression of tumor growth in orthotopic 4T1-bearing mice among the treatment groups. In contrast with single chemotherapy or photothermal therapy, chemo-photothermal treatment by PSC/ICG@+DOX nanoparticles synergistically inhibited the growth of 4T1 cells.Conclusion This study demonstrated that PSC/ICG@+DOX nanoparticles with active targeting and stimuli-sensitivity would be a promising strategy to enhance chemo-photothermal cancer therapy.
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Food Science and Human Wellness
·
2022
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IF 8.022
Hongyuan Zhao,Xiaoyu Chen,Fanqiang Meng,Libang Zhou,Xinyi Pang,Zhaoxin Lu,Yingjian Lu
Hyperuricemia is a critical threat to human health, and a high inosine diet can increase the prevalence of it. Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt, and its inosine degradation rate reached 36.3 % at 10 9 CFU/mL for 24 h. LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L. rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin. The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts (CFE). L. rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase (XOD) inhibition and ATP-binding cassette subfamily G member 2 (ABCG2) promotion, both of which are responsible for uric acid (UA) synthesis and secretion in HepG2 and Caco2 cells, respectively. The in vivo results showed that the serum UA level decreased from 236.28 to 149.28 μmol/L after 8 weeks of oral administration of L. rhamnosus Fmb14 in inosine-induced hyperuricemia model mice. Our results revealed that L. rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
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