CARBOHYDRATE RESEARCH
·
2026
·
IF 3.4
Chenyu Wang,Guannan Yu,Qian Liu,Lichun Ye,Shilei Wang,Guohua Zheng,Junjie Hu,Lei Xu,Yan Meng,Zhaohua Shi
Recently, low-temperature water isolation has been confirmed to better keep the original structure of polysaccharides in plant. Thus, in this work, we obtained a new polysaccharide (WPVP) by water extraction at room-temperature (RT) from Prunella vulgaris L., a famous traditional edible Chinese medicinal herb. Unlike most reported complex heteropolysaccharides from Prunella vulgaris L. by hot water, the polysaccharide we obtained at RT was identified as an arabinoxylan, which is comprised a β -1,4-D-xylopyranose (Xyl p ) backbone substituted at O-2 with linear α -1,5-L-arabinofuranose (Araƒ) side chains. Notably, this new WPVP significantly ameliorated alcohol-induced hepatic damage in mice through gut regulation, as indicated by the improvement on hepatic lipid accumulation, inflammation, and intestinal microecology. Collectively, this work suggests a new strategy for polysaccharides isolation to maintain the original structure, and also proposes a novel therapeutic strategy for alcoholic liver disease (ALD).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.2
Qiqi Feng,Qing Wang,Tao Wang,Hongyu Pan,Yujie Zhong,Yongliang Zhuang
Glycation promotes the generation of advanced glycation end products (AGEs), which in turn exacerbate inflammation, oxidative stress, and chronic metabolic disorders. Given the potential side effects associated with synthetic antiglycation agents, a natural polyphenol-based antiglycation agent was explored from the Tamarindus indica L. shell. In this study, Tamarindus indica L. shell polyphenols (TSP) were obtained via ultrasound-assisted extraction combined with AB-8 macroporous resin purification, which yielded the highest levels of phenolics and flavonoids. UPLC-Q-Orbitrap-MS² analysis identified 23 phenolic compounds, with flavonoids constituting the predominant class, including (+)-catechin, luteolin, and apigenin. TSP exhibited strong antiglycation activity, with dose-dependent inhibition of Amadori intermediates and AGEs. Mechanistic investigations revealed that TSP effectively trapped reactive carbonyl species (RCS), reduced protein oxidation and cross-linking, and maintained protein secondary structure. Spectroscopic analyses and molecular docking further confirmed stable noncovalent interactions between TSP constituents and key glycation-prone residues (arginine and lysine) in bovine serum albumin. Among the identified monomers, (+)-catechin, luteolin, apigenin, diosmetin, 4-hydroxybenzoic acid, procyanidin A2, and eriodictyol-7-O-glucoside exhibited the greatest antiglycation effects. Overall, this study demonstrated that TSP effectively inhibited glycation by trapping RCS, suppressing protein oxidation and structural alteration, and establishing specific interactions with key amino acid residues in proteins. The innovation of this study lies in systematically developing TSP as a novel natural antiglycation agent, demonstrating its multi-mechanistic efficacy and identifying key active constituents, thereby offering a sustainable strategy to valorize agricultural by-products.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 2
·
IF 4.6
Xiaoyan He,Lisheng Tang,Ran Huang
With the growing demand for eco-friendly food packaging, poly(butylene adipate-co-terephthalate) (PBAT)/polylactic acid (PLA) composite films have emerged as promising biodegradable alternatives, but their inherent limitations (e.g., poor antioxidant capacity, weak UV stability, and insufficient antimicrobial activity) hinder practical applications. This study aimed to address these drawbacks by incorporatingRosmarinus officinalisL. extract (RM) as a natural multifunctional additive. PBAT/PLA/RM blend films with RM concentrations of 0.1%, 0.3%, 0.5%, and 1% were fabricated via melt extrusion and blown film processing. Key characterizations were conducted to evaluate thermal stability, mechanical properties, morphology, antioxidant activity, UV-shielding performance, antimicrobial efficacy, and biodegradability. The results showed that RM significantly enhanced the antioxidant capacity of the films, with the highest DPPH radical scavenging activity achieved at 0.3% RM. UV-blocking performance improved incrementally with increasing RM concentration, and films containing ≥0.5% RM filtered over 90% of UVA and UVB radiation. All composite films met biodegradability standards, with over 90% degradation observed after 240 days of composting, though RM prolonged the initial degradation stage by inhibiting early microbial activity. However, the antimicrobial effect of RM was limited, and concentrations exceeding 1% caused film stickiness, impeding processing. This work demonstrates that RM is a viable natural additive for functionalizing PBAT/PLA films, offering enhanced antioxidant and UV-shielding properties while maintaining biodegradability, thus providing a promising solution for sustainable food packaging.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.2
Yuqi Gao,Min Lu,Jitong Wei,Dengyu Zheng,Hongliang Duan,Mingxu Wang,Zhao Ke,Zhongyi Wu,Qing X. Li,Zhongbao Zhang
Flavonoids, secondary phytometabolites, play many roles in human health and plant resistance, growth, and development. They are commonly glycosylated in the last step of their biosynthesis. In this study, a gene encoding an efficient flavonoid glycosyltransferase, ZmUGT76B1, was cloned from maize and expressed. The enzyme activity and function were characterized. ZmUGT76B1 catalyzed selective glycosylation at 3-, 7-, 3′-, and 4′-hydroxyl sites of flavonoids to form mono-O-glycoside and di-O-glycoside products. Moreover, ZmUGT76B1 had a broad substrate preference and strict sugar donor selectivity. Guided by simulated molecular docking and simulated mutagenesis, three ZmUGT76B1 mutants, Q338P, E361P, and Q338P/E361P, were constructed. The mutation of either of the two sites substantially reduced the enzyme activity, whereas the double mutation eliminated the activity. The determination of enzyme activity and prediction of the active site of this enzyme have expanded our knowledge of maize uridine diphosphate-dependent glycosyltransferases, providing an important glycosyltransferase for metabolic engineering of mono- and dioxygenated glucosyl flavonoids.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·Vol. 12
·期 12
·11818-11832
·
IF 1.5
Yuli Wei,Huihui An,Wu Yang,Hao Guo
In this work, a simple, sensitive and rapid electrochemical sensor for bisphenol A determination based on a 3D Au NPs-poly-L-glutamate/graphene nanocomposite was proposed and the structures of the nanocomposite were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopic (FTIR) and X-ray diffraction (XRD). The electrochemical behaviors of bisphenol A on the nanocomposite GCE were investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). In the potential range of 0.0 ~ 1.0 V, an irreversible oxidation peak appeared on the modified electrode in 0.1 mol L -1 phosphate buffer solution (pH = 6.0). Under the optimum conditions, the anode peak current of bisphenol A increased linearly with the its concentration in the ranges from 0.009 μmol L -1 to 0.1 μmol L -1 and 0.1 μmol L -1 to 1.0 μmol L -1 with the detection limit down to 3×10 −10 mol L -1 (S/N = 3) and the corresponding linear regression equations were Ipa ( μA)= − 0.1129 C ( μmol L -1 ) + 0.5532 (N = 6, R 2 = 0.9057) and Ipa ( μA) = − 0.05970 C ( μmol L -1 ) + 0.02182 (N = 5, R 2 = 0.9950) respectively. The proposed method was successfully used for the determination of bisphenol A content in wastewater and milk samples, and the recoveries were in the range from 98.10% to 104.9%.
研究领域:
分析化学
·
环境监测
·
食品安全
·
电化学传感
·
纳米生物技术
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9
Ruohan Liang,Dongsheng Zhao,Fangming Jin,Jun Xu,Guicai Liu
The limited mono−/divalent anion selectivity and inadequate antifouling properties of polyamide (PA) nanofiltration (NF) membranes restrict their use in water treatment. Herein, L-histidine (L-His)-modified NF membranes were prepared through interfacial polymerization on a polyethersulfone porous substrate. The aqueous-phase comonomers were piperazine and L-His, which contain both amino and carboxyl groups, while the organic-phase monomer was trimesoyl chloride. Compared with the NF0 membrane without addition of L-His, all of the L-His-modified membrane was characterized by a higher surface roughness, a thinner PA layer, greater hydrophilicity, a more negative surface charge and a larger mean pore size. Among them, the optimal NF0.5 membrane with the addition of 0.5% ( w / v ) L-His demonstrated a pure water permeability of 17.9 L/(m 2 ·h·bar) and a 2.7-fold higher NaCl/Na 2 SO 4 selectivity (up to 102.0). In particular, the NaCl/Na 2 SO 4 selectivity improved with higher operating pressure, but decreased with increasing single-salt concentration or NaCl/Na 2 SO 4 mass ratio in the feed solution. In addition, the NF0.5 membrane demonstrated superior resistance to protein fouling and outstanding long-term operational stability throughout a continuous 7-day filtration process. This study could provide valuable insights into the development of NF membranes with high mono−/divalent anion selectivity and excellent antifouling properties.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.1
Xiao-Han Yuan,Wen-Sheng Liu,Jian-Hua Zhang
Formaldehyde (FA), the simplest and widely-applied aldehyde, can pose serious health issues when present at elevated concentrations. Herein, this study proposes a self-calibrating ratiometric sensing strategy for the sensitive detection of FA, which is based on the dual-functional ligands and multi-metal synergistic effects of L-Histidine (L-His)-functionalized Lanthanide metal-organic gels (MOGs). In this strategy, L-His and 1,3,5-benzenetricarboxylic acid (BTC) served as functional ligands coordinating with Zr 4+ , Eu 3+ , and Tb 3+ metal centers, forming three MOGs with superior photoluminescence properties: ZrEuTb-His-MOG, ZrEu-His-MOG, and ZrTb-His-MOG. Notably, ZrEuTb-His-MOG exhibits a prominent multi-metal synergistic effect: a stable self-calibration signal is generated through Zr 4+ -(L-His) ligand-to-metal charge transfer (LMCT), while the antenna effect of BTC to Tb 3+ facilitates a FA-responsive specific fluorescent signal. The synergistic interaction between these two mechanisms establishes a highly reliable self-calibrating detection system. This sensor achieves high sensitivity with a detection limit of 8.4 nM, a broad linear range of 0.001 μM–60 μM, and excellent selectivity against common interfering species. Moreover, ZrEuTb-His-MOG exhibits outstanding regenerability and long-term signal stability, qualifying it as an efficient and recyclable fluorescent probe for FA detection. Additionally, we developed a portable fluorescent test strip based on ZrEuTb-His-MOG, enabling “on-off” visual detection of both aqueous and gaseous FA with a fast response. This work provides a novel and reliable strategy for on-site FA monitoring in environmental monitoring, food safety, and public health protection.
✓ 已验证
产品关联
DOI
高影响因子
BIOORGANIC CHEMISTRY
·
2026
·
IF 5.1
Xianying Fang,Wenhui Zhu,Lanxin Lu,Lulu Wu,Yufeng Zhang,Suxiang Liu,Wuyang Huang,Linguo Zhao
With the increasing popularity of olive oil, the cultivation area of Olea europaea L. (olive) trees has been expanding, and the processing and utilization of olive leaves are gaining more attention. Besides oleuropein, the main active compounds in olive leaves include luteolin and its glycosides. Through fractionation, a component rich in key active substances, designated OLE, was prepared from olive leaves. Our activity analysis showed that luteolin exhibits excellent DPPH radical scavenging and higher anti-inflammatory activities compared to its glycosides. To enhance the activity of OLE, a glycosidase Bbxyl was screened, identified and used to catalyze OLE. Luteolin-5- O -glucoside, luteolin-7- O -glucoside, luteolin-4’- O -glucoside, and luteolin-3’- O -glucoside in OLE were hydrolyzed into luteolin with a conversion rate of 94%. OLE hydrolyzed by Bbxyl (OLEB), with a luteolin content of approximately 5.93 mg/g, was obtained through macroporous resin purification. The anti-inflammatory activity mediated by macrophages and T cells of OLEB was increased by 51% and 39%, respectively. In a DSS-induced mouse colitis model, OLEB significantly alleviated intestinal inflammation symptoms, promoted weight recovery, reduced spleen swelling, restored colon length, repaired the intestinal epithelial barrier, decreased the oxidative and inflammatory levels, outperforming both the positive control drug 5-ASA and OLE. The primary mechanism of action likely involves components such as luteolin in OLEB inhibiting the release of inflammatory factors by blocking the TLR4-NF-κB/AHR pathway, thereby mitigating pathological damage in the colon. This study provides an innovative processing approach for the development of high-value olive leaf products with enhanced anti-inflammatory activity and intestinal health-protective functions.
✓ 已验证
DOI
PubMed
高影响因子
SYNTHETIC METALS
·
2026
·
IF 4.5
Wardah Ahmed,Qurat-ul-ain Shaikh,Bilqees Hussain,Muhammad Yousif,Sadam Hussain,Abdul Ghaffar,Kai Wei
One-step electrospinning via NIPS produced highly porous PLLA membranes. • Achieved high solar reflectance (∼95.7%) and strong mid-IR emission. • Porous morphology enabled 2.6–3.9 °C cooling vs smooth PLLA. • Up to 9.2 °C reduction compared with cotton under sunlight. • Scalable, low-cost route for flexible daytime radiative cooling textiles. The growing demand for efficient and cost-effective cooling solutions, both indoors and outdoors, especially for personal cooling in outdoor environments, posses a significant global challenge. Current cooling methods often rely on custom optical structures with spectral selectivity, which typically involve complex manufacturing processes, limiting their large scale production and cost-effectiveness. This study aims to develop a radiation cooling material by utilizing the porous structure of poly- L -lactic acid (PLLA) through one-step electrospinning method based on the Non-solvent induced phase separation (NIPS) technique. The resulting membrane, with porous structure, exhibits substantial roughness and a high specific surface area, revealing an impressive average solar reflectance (∼95.7%) under wavelength of (0.25 ∼ 2.5 μm) and mid infrared emittance (∼88.3%) under wavelength of (8 ∼ 13 μm). The study evaluates the indoor cooling performance of porous PLLA, resulting in temperature reductions of approximately 2.6∼3.9°C and 5∼9.2°C compared to smooth PLLA and cotton-covered surface, respectively, under different solar intensities. It also explores the potential of porous PLLA for outdoor cooling applications, emphasizing its hydrophobic, mechanical, and flexible properties. It proposes a cost-effective method for producing high-performance radiative cooling materials using PLLA and underscores its versatility for practical applications.
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2026
·Vol. 15
·期 13
·2396
·
IF 6
Huiling Huang,Zhichao Yang,Lin Ye,Xujie Hou,Yiming Jia,Shenghuizi Chen,Ying Huang
In this study,Apocynum venetumwas employed as the raw material to optimize protein extraction and enzymatic hydrolysis processes for the preparation of highly active angiotensin-converting enzyme (ACE)-inhibitory peptides, achieving an ACE inhibition rate of 92.34%. Multispectral analyses and microstructural characterization demonstrated that enzymatic hydrolysis induced the unfolding of protein secondary structures, resulting in a looser and more porous morphology enriched with characteristic amino acids. A total of 2567 peptide sequences were identified by LC–MS/MS, among which 18 potential bioactive peptides were screened. Molecular docking analysis revealed that these peptides interact with the active site of ACE primarily through hydrogen bonding and hydrophobic interactions, with WLRDFL exhibiting the strongest binding affinity. This study systematically elucidates the structural characteristics and antihypertensive molecular mechanisms of ACE-inhibitory peptides derived fromApocynum venetum, providing both theoretical insights and experimental support for the development of natural antihypertensive functional foods and the high-value utilization of this plant.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2026
·
IF 4
Khalid Bashir,Guozhang Bao,Shoujat Ali,Jinke Hu,Yue Yuan,Lan Bao,Sana Shireen
BACKGROUND Environmental stresses such as extreme temperatures, salinity, drought, metal toxicity and freeze–thaw (FT) cycles severely limit crop productivity. The increasing frequency of FT events combined with aluminum (Al) toxicity, exacerbated by global warming, poses a serious threat to seedling survival, especially in early spring. Brassinolide (BL), a phytohormone, enhances plant resilience under abiotic stress, yet its role under combined FT and Al stress in rye ( Secale cereale L.) remains unexplored. RESULTS Al stress increased malondialdehyde content, indicating membrane lipid peroxidation, at the same time as reducing chlorophyll content, photosynthesis and transpiration. Combined Al + FT stress elevated intercellular CO₂ concentration and soluble sugars but lowered stomatal conductance and water-use efficiency, revealing biochemical limitations to photosynthesis. BL alone restored stomatal conductance, lowered intercellular CO₂ and enhanced water-use efficiency, improving CO₂ fixation. Al stress produced the highest H₂O₂ accumulation under both non-FT and FT conditions alongside compensatory increases in ascorbate peroxidase (APX) activity, reflecting an overwhelmed antioxidant response; co-application of BL with Al reduced H₂O₂ and moderated this APX elevation relative to Al alone. BL alone increased glutathione content above all treatments, although this benefit was partly constrained when combined with Al under FT. BL also improved shoot relative water content, photosynthetic efficiency and soluble sugar regulation, promoting osmotic adjustment and cold tolerance. CONCLUSION BL enhances rye tolerance to combined FT and Al stress by restoring photosynthetic function and modulating antioxidant responses, offering a promising approach to safeguard crop productivity in regions prone to such abiotic challenges. © 2026 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Junxi Liu,Yan Liu,Yuheng Zheng,Haoyu Wang,Jinglin Wang,Yu Zhang,Kaiping Wang
SCLP remained intact in the stomach and small intestine after oral administration. • SCLP was degraded into oligosaccharide fragments in the cecum and colon. • The intestinal metabolism of SCLP was gut microbiota-dependent. • Phocaeicola vulgatus and Bacteroides uniformis were involved in SCLP metabolism. This study aimed to investigate the intestinal metabolic characteristics and mechanisms of the pectic polysaccharide isolated from the medicinal plant Smilax china L. (SCLP). Firstly, in vitro simulated digestion confirmed that SCLP remained stable in simulated digestive fluids. Subsequently, in vivo real-time tracking of intestinal metabolism based on fluorescent labeling revealed that SCLP maintained its prototype in the small intestine and began to be degraded into fragments (Mw < 4000 Da) upon reaching the cecum and colon, where it was retained for prolonged periods. Pseudo-sterile mouse experiments indicated the mediating role of gut microbiota in SCLP metabolism. Furthermore, metagenomic sequencing suggested that SCLP increased the proportion of polysaccharide utilization loci (PULs) from Phocaeicola vulgatus and Bacteroides uniformis , elevated the gene numbers of carbohydrate-active enzymes (CAZymes) including GHs, GTs and CBMs, and activated pathways of carbohydrate metabolism. Finally, in vitro bacterial culture study verified the degradation and utilization of SCLP by Phocaeicola vulgatus and Bacteroides uniformis . In summary, this work elucidates the intestinal metabolic profile of SCLP, providing valuable insights for its further development and utilization.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）