ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2026
·
IF 4.1
Wang Wan,An Yingying,Chen Fangfang,Zhang Qing
Adiponectin serves as a critical biomarker for metabolic disorders, with its concentration in human plasma providing predictive value for the risk of prediabetes. In this work, we report a rapid and reliable electrochemical immunosensor designed for point-of-care testing of adiponectin. The immunosensor was fabricated using a gold-sputtered silver screen-printed electrode as the substrate, on which sulfhydrylated antibodies were directly immobilized via Au-S covalent bonding. The fabrication process was characterized and optimized using electrochemical impedance spectroscopy and differential pulse voltammetry. Under optimal conditions, the proposed immunosensor exhibited a wide linear range (0.3–15 μg/mL) with a detection limit of 0.15 μg/mL. It also demonstrated excellent reproducibility and high selectivity for adiponectin detection. Additionally, the proposed immunosensor can be fabricated within 1.5 h and enables accurate quantification in human plasma within 15 min. The practical applicability of the sensor was validated through analysis of human plasma samples, and the comparison results with commercial ELISA kit are satisfactory. Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Dong-Mei Liu,Juan Chen,Yan-Ping Shi
In the present study, the immobilization method of physical adsorption combined with covalent crosslinking was developed to avoid the shortcomings of both the noncovalent and covalent coupling methods. For the first time, tyrosinase (TYR) was immobilized on the surface of aminated magnetic nanoparticles (Fe 3 O 4 -NH 2 ) by the developed method. TYR was firstly adsorbed on the surface of Fe 3 O 4 -NH 2 by electrostatic interaction , and then by covalent crosslinking with glutaraldehyde (GA), TYR was firmly immobilized on the supports. The immobilized TYR showed enhanced pH and temperature endurances at the optimum pH of 7.0 and temperature of 35 °C. TYR reusability was significantly improved after immobilization and it retained 61.4 ± 2.3% of its initial activity after 5 repeated cycles. Immobilized TYR also showed improved storage stability (73.2 ± 1.1% after 30 days of storage at 4 °C). In addition, the immobilized TYR showed a higher biological affinity to substrate owing to the stabilization of TYR in its active conformation by electrostatic interaction prior to covalent crosslinking. Finally, the immobilized TYR was used to screen of enzyme inhibitors from 11 traditional Chinese medicines (TCMs) to validate whether this method can be used for enzyme inhibitor screening or not.
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Jiwei Song,Yunpeng Shao,Yuan Yao,Zicheng Zhang,Yaqin Ren,Linyu Mei
Surface-enhanced Raman scattering (SERS) has emerged as a powerful analytical technique for ultrasensitive and rapid detection. However, the performance of SERS substrates largely depends on their ability to generate intense localized electromagnetic fields at nanoscale hot spots, while simultaneously achieving high sensitivity and signal reproducibility remains a major challenge. In this work, a highly ordered SU-8 micropillar array was fabricated by Ultraviolet Lithography(UL), followed by biomimetic modification with polydopamine (PDA) to achieve the uniform immobilization of silver nanoflowers (AgNFs). The resulting three-dimensional (3D) AgNFs/PDA/SU-8 micropillar SERS substrate integrates the structural advantages of the 3D architecture, the abundant electromagnetic hot spots and physical enrichment effect generated by densely packed AgNFs, and the interfacial regulation provided by PDA, thereby synergistically enhancing the SERS performance. The substrate exhibited an enhancement factor(EF) of 2.16 × 10 9 for Rhodamine 6G (R6G), with an experimental limit of detection as low as 1 × 10 −14 M (S/N = 3) and a relative standard deviation below 8.30%. Furthermore, the limits of detection for enrofloxacin (ENR), clothianidin (CLO), and 100 and 800 nm polystyrene (PS) particles in seawater reached 1 × 10 −13 M, 1 × 10 −14 M, 2.5 μg mL −1 , and 0.6 mg mL −1 , respectively. Simultaneous detection of multiple contaminants was also successfully achieved in complex food matrices, including mineral water, milk, and liquor. This work provides an effective strategy for constructing high-performance 3D SERS substrates and demonstrates their promising potential for food safety monitoring and aquatic environmental analysis.
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2026
·Vol. 42
·期 30
·22403-22412
·
IF 4.4
Cai Jixiang,Zhao Haiyan,Qin Jiannan,Li Mingfu,Zhang Pingjun,Jiang Liqun
Metal–organic frameworks (MOFs) represent promising
platforms
for enzyme immobilization; however, the interplay between surface
chemical heterogeneity and defect density in dictating adsorption
efficiency remains poorly understood. Herein, we systematically investigated
the immobilization of glucose isomerase (GI) on three UiO-66 variants,
indicating that the acetic acid (AA)-modified UiO-66 (UiO-66-AA) achieved
the highest enzyme loading efficiency of 92.8% and activity recovery
of 84.2%, markedly outperforming both pristine UiO-66 and formic acid-modified
UiO-66 (UiO-66-FA). The resulting UiO-66-AA-GI composite achieved
the highest fructose yield of 37.7% with an excellent selectivity
of 94.8% under optimal conditions and retained stable performance
over five recycling cycles. Comprehensive characterization demonstrates
that acetate modification generates a distinctive chemical mosaic
surface, in which hydrophobic methyl domains and residual carboxyl
groups synergistically enable multimodal adsorption via hydrophobic
interactions, hydrogen bonding, and weak electrostatic forces. This
unique surface chemistry, combined with the largest external surface
area, maximizes GI loading while preserving the catalytic activity.
These findings establish that rationally designed surface chemical
mosaics with complementary interaction modes are critical for achieving
high-efficiency enzyme immobilization in MOF-based biocatalysts.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Xiaochu Chen,Minxing Huang,Jianbin Chen,Xiaopeng Wang,Qiaoyun Cheng,Zhaohua Ping,Guihua Wang,Yufeng Gao
The enzyme-based colorimetric sensor allows rapid and visual detection of food contaminants but is limited by low sensitivity and impurity interference. In the present work, a colorimetric biosensor for atrazine detection was fabricated through oriented immobilization of tyrosinase onto magnetic polystyrene nanospheres (MPSNPs). MPSNPs carriers were prepared by surface amination of monodisperse polystyrene nanospheres, followed by concanavalin A modification to introduce specific affinity sites for glycosyl groups on tyrosinase. Then, tyrosinase was bound to these specific sites via affinity self-assembly. The obtained MPSNPs immobilized tyrosinase (MPSNP-ConA/Tyr) were characterized and displayed uniform and stable structure, excellent enzyme activity, and good adsorption properties for the target atrazine. The MPSNP-ConA/Tyr, combined with 4-aminoantipyrine/catechol colorimetric indicator and smartphone, was used to analyze atrazine in three vegetable samples. The method enabled atrazine enrichment, enzyme activity inhibition reaction, and quantitative analysis in a single platform. This MPSNP-ConA/Tyr based method exhibited good linearity in the range of 0.1-2.0 mg L −1 (γ > 0.9953) with a limit of detection (LOD) of 0.054 mg L −1 . The accuracy evaluated by recoveries ranged from 73.1% to 100.5% with relative standard deviations (RSDs) of 1.2-12.5%. A comparison with the GC-MS/MS method was performed using real vegetable samples (cucumber, carrot, and lettuce), and comparable atrazine concentrations were obtained, validating the practical applicability of the proposed method.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Digest Journal of Nanomaterials and Biostructures
·
2026
·Vol. 21
·期 1
·
IF 1.2
Rong Zhou,Jian Ding
A magnetic biochar–nanoscale zero-valent iron (MBC-nZVI) composite was synthesized from pine bark via an in-situ liquid-phase reduction method to immobilize arsenic in mining-impacted soils. The optimized MBC-40 exhibited uniformly dispersed Fe(0)@Fe3O4 nanoparticles (50–80 nm) anchored on a graphitic biochar scaffold, combining a high surface area (180.9 m2 g-1) with strong magnetization (32.5 emu g-1). Batch experiments revealed outstanding performance, achieving 98.5% As(V) and 96.5% As(III) removal under neutral conditions, with equilibrium reached within 120 min. Adsorption kinetics were well described by the pseudo-second-order model (R2 >0.998) and equilibrium data were consistent with the Langmuir model, yielding maximum capacities of 65.4 mg g-1 for As(V) and 82.1 mg g-1 for As(III). Based on the observed pH dependence, zeta-potential/point of zero charge (PZC) context, and competitive anion effects, the arsenic immobilization behaviour is consistent with contributions from electrostatic interactions (particularly for anionic As(V) under pH < PZC) and specific interactions with iron (hydr)oxide surfaces. Potential redox involvement of Fe(0) is discussed as mechanistically plausible for nZVI-based systems, but arsenic/iron speciation was not directly measured in this study. The composite maintained >81% efficiency after five regeneration cycles and displayed excellent selectivity in the presence of competing anions, with minimal interference from sulfate and chloride but moderate competition from phosphate. When tested in simulated mining wastewater containing 5.0 mg L-1 total arsenic and high levels of Pb2+, Cd2+, and SO42-, the MBC-40 achieved 97.0% arsenic removal, >99.9% Pb2+ elimination, and 95.6% Cd2+ reduction. The rapid magnetic separation from treated water within 30 s enables simple recovery of the sorbent using an external permanent (neodymium) magnet, without requiring electrically powered separation equipment. These findings demonstrate that the MBC-nZVI composite mitigates key practical limitations of bare nZVI (aggregation/passivation and post-treatment recovery) by integrating a biochar scaffold with a magnetically recoverable Fe(0)@Fe3O4 architecture. Consistent with prior reports of nZVI/biochar (BC)-type systems, the present work contributes an application-focused validation primarily: performance is quantified across Fe-loading levels and confirmed in a mining-relevant mixed-ion/multi-metal matrix (high sulfate and co-occurring Pb2+/Cd2+), together with reusability supported by permanent-magnet separation.
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Cheng Peng,Kailin Gong,Qiannan Li,Weiyu Liang,Huihui Song,Fang Liu,Jie Yang,Wei Zhang
Magnesium-aluminum modified biochar (MABs) has an outstanding effect on the simultaneous immobilization of arsenic (As), lead (Pb), and cadmium (Cd) in soil, but the stability of remediation effect of MAB under various natural conditions is still unknown. In this study, we investigated the effects of organic acids, dry-wet cycles (DW), freeze-thaw cycles (FT), and rainfall (pH 4, 7, and 8) on the immobilization of As, Pb, and Cd by MAB. The results showed that oxalic acid decreased the immobilization efficiencies of As, Pb, and Cd by 15.5%–38.5%; meanwhile, humic acid reduced the immobilization efficiency of Pb by 89.7%, but elevated that of Cd by 19.5%. The immobilization mechanisms of MAB-5 on three metals were mainly involved in ion exchange and surface-complexation. Compared with the 7th round, the immobilization efficiencies of As, Pb, and Cd by MAB in the 28th round was decreased by 17%–28% in DW, but was increased by 11%–18% in FT. In addition, MAB was transformed into hydrotalcite after FT and DW. After experiencing simulated rainfall, MAB caused more As, Pb, and Cd to be retained in the upper soil layer, and the immobilization effect of MBA was more significant under the stimulated rainfall with higher pH. The study provides a more theoretical basis for the application of MAB in the actual site remediation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Ying Tang,Penghui Wang,Hui Zeng,Zebao Rui
Fabrication of highly-efficient enzymatic supports having excellent affinity to enzymes and superior mass transfer properties is highly desirable for enzymatic bio-catalysis. Herein, newly engineered chitosan macrospheres having interconnected and interlaced network pores are prepared via dual pore-forming strategy and applied as novel host for the effective immobilization of alkaline protease. The synergetic effect of SiO 2 templates and gas-induced pore-forming agents play an important role in inhibiting the over-crosslinking of chitosan chains and promoting the elevation of interior porosity. Benefited from the highly exposed surface and abundant available binding sites, the as-developed porous support P 2 CSM achieves a maximum loading capacity of 43.8 ± 0.8 mg/g and ultra-high activity recovery of 92.4 % for alkaline protease. P 2 CSM is competent to effectively stabilize the structural conformation of alkaline protease from inactivation through the flexible covalent interaction. Considering these attributes, Protease@P 2 CSM demonstrates remarkably better structural stability, reusability and SDS-resistance than free alkaline protease, as well as excellent proteolytic ability, and the residual activity of Protease@P 2 CSM is evaluated as high as 70.3 % after 7 consecutive reuses. This work provides a promising avenue to construct highly-active enzyme-composites for widespread utilization in various practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Qidong Yu,Yaping Wu,Wenmin Zhang,Wende Ma,Juan Wang,Hui Chen,Qingqing Ding,Lan Zhang
Rapid and convenient preparation of covalent organic framework (COF) coated fibers is of great significance to solid-phase microextraction (SPME) technology. In this work, a novel chemical preparation strategy was established for rapid fabrication of β -ketoenamine-linked COF coatings, in which clay-like starting materials of COFs were first wrapped on the fiber surface through self-viscosity and further fixed on the fiber in an oven via chemical bonding. Based on this strategy, four different COF (TpTph, TpPa-1, TpBD and TpTpb) coated fibers was fabricated within 1 h, which is very rapid compared to the recently reported research. Moreover, the strategy also demonstrates the good general applicability for COF fiber preparation. Subsequently, the TpTph coated fiber was used to develop a new SPME method for gas chromatography-tandem mass spectrometry (GC-MS/MS) of trace phthalic acid esters (PAEs) in environmental water. The developed analytical method compared to the previous SPME methods for PAEs based on other sorbents possesses low limits of detection (LODs, 0.02–0.08 ng L −1 ), and better or comparable precision (RSD ≤9.4%, n = 6), and relatively short extraction time. Furthermore, the trace PAEs (0.27–11.62 ng L −1 ) in the real water samples were successfully detected with recoveries of 82.2–117.5%. The above results indicates that the proposed fiber preparation strategy is reliable and opens a potential avenue for rapid and facile fabrication of COF coated fibers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
·
2021
·
IF 9.642
Xiao-Man Fan,Jia-Jia Shen,Yuan-Yuan Xu,Jian Gao,Ye-Wang Zhang
A method for specific immobilization of whole-cell with covalent bonds was developed through a click reaction between alkyne and azide groups. In this approach, magnetic nanoparticle Fe 3 O 4 @SiO 2 -NH 2 -alkyne was synthesized with Fe 3 O 4 core preparation, SiO 2 coating, and alkyne functionalization on the surface. The azides were successfully integrated onto the cell surface of the recombinant E. coli harboring glycerol dehydrogenase, which was employed as the model cell. The highest immobilization yield of 83% and activity recovery of 94% were obtained under the conditions of 0.67 mg mg −1 cell-support ratio, pH 6.0, temperature 45 °C, and 20 mM Cu 2+ concentration. The immobilized cell showed good reusability, which remained over 50% of initial activity after 10 cycles of utilization. Its activity was 9.7-fold higher than that of the free cell at the condition of pH 8.0 and each optimal temperature. Furthermore, the immobilized cell showed significantly higher activity, operational stability, and reusability.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Teng Sun,Zhe Dong,Jing Wang,Feng-Hong Huang,Ming-Ming Zheng
Lipase immobilization processes have the potential to drive large-scale industrial applications through cost savings. Among them, the Pickering emulsion system has recently emerged as an ecofriendly technique for enhanced enzymatic activity. In this study, a novel and facile immobilization of lipase in an ultrasound-assisted Pickering emulsion system was developed. The lipase loading amount and yield was 177.3 mg/g and 41.3%, respectively. In the enzymatic synthesis of four sterol lipoates, the immobilized lipase (UIP-HMSS@CRL) achieved the highest catalytic efficiency (CE) of 6.4 mmol/g·h among the reported methods, which was 9.1-fold higher than that of free lipase and 32.0-fold higher than that of previous methods. UIP-HMSS@CRL performed with much higher Vm and lower Km, indicating the excellent catalytic rate and stronger affinity. Moreover, UIP-HMSS@CRL displayed satisfying thermal stability and recyclability, retaining 88.6% of enzyme activity after 10 cycles. The lipase lid was opened at the oil–water interface by both ultrasound and interfacial synergistic activation effect, which provided easier access to an active site and thus achieved excellent catalytic performance. This ultrasound-assisted interface immobilization would open new opportunities for preparation of high-performance and sustainable biocatalysts.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Wei Huang,Shuai Pan,You Li,Lulu Yu,Ruijiang Liu
In this work, magnetic Fe 2 O 3 /Fe 3 O 4 nanocomposites were prepared via a novel rapid combustion process. The silica was precipitated on the surface of Fe 2 O 3 /Fe 3 O 4 nanocomposites. The silica-coated magnetic nanocomposites were cross-linked with glutaraldehyde, on which cellulase was covalently immobilized. The morphology, composition, and property of the prepared nanomaterials were characterized by the scanning electron microscopy (SEM), the energy dispersive spectrometry (EDS), the X-ray diffraction (XRD), the vibrating sample magnetometer (VSM), and the Fourier transform infrared (FTIR) spectroscopy. The immobilization conditions were optimized by varying operating parameters and determined to be 0.05 mL of 0.5% cellulase solution for 2 h. The catalytic stabilities of the immobilized cellulase were evaluated. The results showed that the immobilized cellulases performed higher apparent activity at pH 4.5 and exhibited good thermal stability compared with their free counterparts. The Michaelis-Menten equation showed that K m and V max of free cellulase were 3.46 mol·L −1 and 0.53 mol·min −1 , respectively. The immobilized cellulase had higher K m and V max (18.99 mol·L −1 and 0.59 mol·min −1 ). The retained activity of the immobilized cellulase maintained over 71% of the initial activity after being used for five cycles.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）