JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Zizhe Li,Jinkui Wang,Youyi Wang,Qingyue Li,Xiaoyang Chen,Yu Wang,Haining Tian,Yexuan Mao,Lianjun Song,Xianqing Huang,Zhanhui Wang,Xiya Zhang
To avoid false-positive results in immunoassays due to cross-reactivity of antibodies with structural analogues, especially metabolites of target compounds, the preparation of highly specific antibodies is crucial. Preserving the characteristic structure of a target compound when designing a hapten is important when preparing highly specific antibodies. Here, we designed a novel hapten, 4-(((1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4yl)amino)methyl)benzoic acid, named AA-BA, to improve the specificity of antibodies for detection of 4-methylaminoantipyrine (MAA), a residual marker of dipyrone, an important antipyretic-analgesic and anti-inflammatory drug. The structural features of the hapten remained almost the same as those of MAA. After experimental validation, monoclonal antibody 6A4 (mAb 6A4) was prepared with the half maximal inhibitory concentration (IC50) value of 4.03 ng/mL and negligible cross-reactivity with dipyrone metabolites and other antibiotics. In addition, a specific lateral flow immunoassay (LFA) strip based on colloidal gold was developed for screening MAA with a cutoff value of 25 ng/mL in milk. The developed LFA is a useful tool for rapid and accurate detection of MAA.
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IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
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2023
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IF 3.3
Shiling Hu,Jue Wang,Haoyun Bai,Chaohua Feng,Zhenqi Zhou,Zhuoyin Xue,Wen Zhang,Yongjing Zhang,Nan Wang,Langchong He
Background Mast cells (MCs) are important effector cells in anaphylaxis and anaphylactic disease. 3’,4’,5,7-tetrahydroxyflavone (THF) presents in many medicinal plants and exerts a variety of pharmacological effects. In this study, we evaluated the effect of THF on C48/80-induced anaphylaxis and the mechanisms underlying its effects, including the role of secreted phosphoprotein 1 (SPP1), which has not been reported to IgE-independent MC activation.Results THF inhibited C48/80-induced Ca2+ flow and degranulation via the PLCγ/PKC/IP3 pathway in vitro. RNA-seq showed that THF inhibited the expression of SPP1 and downstream molecules. SPP1 is involved in pseudo-anaphylaxis reactions. Silencing SPP1 affects the phosphorylation of AKT and P38. THF suppressed C48/80-induced paw edema, hypothermia and serum histamine, and chemokines release in vivo.Conclusions Our results validated SPP1 is involved in IgE-independent MC activation anaphylactoid reactions. THF inhibited C48/80-mediated anaphylactoid reactions both in vivo and in vitro, suppressed calcium mobilization and inhibited SPP1-related pathways.
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Journal of Physical Chemistry C
·
2023
·
IF 3.7
Shuming Chen,Jintao Wang,Zhenyu Wang,Ziqiang Chen,Kai Fang,Chenyang He,Ning Jiang,Jian Zhang,Ye Li,Chuannan Li,Peng Li,Wenlong Jiang,Yu Duan
The energy level alignment and conductivity of hole transport layers (HTLs) are critical to the performance of perovskite solar cells (PSCs). Additive engineering is an effective approach, and we introduced tri(4-trifluoromethylphenyl)phosphine (343FP) as an additive into poly[bis(4-phenyl)(2,4,6-trimethylphenyl)amine] (PTAA). Studies have shown that the addition of 343FP can lead to improved charge transport properties in the PTAA and can also enhance charge transport, leading to improved device efficiency of the resulting perovskite solar cell. 343FP reduces the highest occupied molecular orbital energy level of PTAA, improves the conductivity of HTLs, and passivates the interface defects, and the optimized device of 343FP shows a champion efficiency of 20.02%. In addition, due to the hydrophobicity of 343FP, compared with the traditional hygroscopic dopant lithium bis((trifluoromethyl)sulfonyl)azide (Li-TFSI)/4-tert-butylphenol (t-BP) bi-doped (Li-doped) PTAA and the undoped PTAA, the device with 343FP-doped PTAA has better environmental stability. After 1000 h of exposure to the environment, the efficiency of 78% of the original value can be maintained. This work will open up a new method for the optimization of high-efficiency and high-stability perovskite photovoltaic hole transport layers.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2023
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IF 4.2
Qilong Peng,Yu Chen,Diyi Fang,Chuanlei Liu,Kongguo Wu,Yuxiang Chen,Hao Jiang,Yuanyuan Sun,Qiumin Wu,Di Wu,Hui Sun
ETS-4 shows great potential to achieve effective separation of challenging mixtures due to its promising tunability of pores. However, the regulation of pore size is largely limited by its low thermal stability. Here, Na-ETS-4 was synthesized by the hydrothermal method and additionally modified via ion exchange coupled with thermal treatment. Structures of all synthesized samples were examined using a variety of characterization techniques. Both adsorption equilibrium and kinetics of CO2 and CH4 on these adsorbents were evaluated on custom-made adsorption units. In addition, the mechanism of CO2 adsorption on different structural models of ETS-4 was revealed via combining with the molecular dynamics (MD) simulations. Ion exchange largely improves the thermal stability of ETS-4 and its adsorption capacities for CO2 and CH4. Ba-ETS-4-350 exhibits the largest equilibrium selectivity of 12.42 and the greatest Henry’s selectivity of 2178.51. The radial distribution function (RDF) results reveal the main adsorption sites of CO2 in different ETS-4 structures.
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CHEMOSPHERE
·
2023
·
IF 8.8
Xuan Zhang,Keke Geng,Ningning Wu,Gang Hu,Ben Fan,Jian He,Wenjing Qiao
4-Chloro-2-methylphenoxyacetic acid (MCPA) is a widely used herbicide across the world. MCPA is persistent and easily transports into anoxic environment, such as groundwater, sediments and deep soils. However, little research on anaerobic microbial degradation of MCPA was carried out. The functional microorganisms as well as the catabolic pathway are still unknown. In this research, an anaerobic MCPA-degrading bacterial consortium was enriched from the river sediment near a pesticide-manufacturing plant. After about 6 months’ acclimation , the MCPA transformation rate of the consortium reached 4.32 μmol g −1 day −1 , 25 times faster than that of the original sludge . 96% of added MCPA (2.5 mM) was degraded within 9 d of incubation. Three metabolites including 4-chloro-2-methylphenol (MCP), 2-methylphenol (2-MP) and phenol were identified during the anaerobic degradation of MCPA. An anaerobic catabolic pathway was firstly proposed: firstly, MCPA was transformed to MCP via the cleavage of the aryl ether, then MCP was reductively dechlorinated to 2-MP which was further demethylated to phenol. The 16S rRNA gene amplicon sequencing revealed a substantial shift in the bacterial community composition after the acclimation. SBR1031, Acidaminococcaceae , Aminicenantales , Syntrophorhabdus , Acidaminobacter , Bacteroidetes_vadinHA17 , Methanosaeta , Bathyarchaeia , KD4-96, Anaeromyxobacter , and Dehalobacter were significantly increased in the enriched consortium after acclimation, and positively correlated with the anaerobic degradation of MCPA as suggested by heat map correlation analysis. This study provides a basis for further elucidation of the anaerobic catabolism of MCPA, and contributes to developing efficient and low-cost anaerobic treatment technologies for MCPA pollution.
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Sustainability
·
2023
·Vol. 15
·期 7
·6061
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IF 3.9
Xiaona Wang,Haishu Sun,Yonglin Wang,Fangxia Wang,Wenbin Zhu,Chuanfu Wu,Qunhui Wang,Ming Gao
This study investigated the feasibility of direct butanol production from starchy food waste (without saccharification and nutrient supplementation). First,Clostridium saccharoperbutylacetonicumN1-4 was selected as an efficient starch-utilizing clostridia, and amylose was used by the strain more readily than amylopectin for solvent production. Furthermore, direct fermentation avoided substrate inhibition due to saccharification and produced 12.1 g/L of butanol at a production rate of 0.705 g/L/h and a yield of 0.402 C-mol/C-mol with a solid–liquid ratio of 1:1 (w/v). At a solid–liquid ratio of 1:2 (w/v), the maximum butanol production rate in the direct mode was 2.05 times higher than that in the saccharified mode. Elemental analysis demonstrated that the food waste analyzed was rich in trace elements and, hence, exogenous nutrient supplementation was unnecessary. Collectively, direct butanol production from food waste could function as a low-cost, highly efficient, and simple fermentative process, which is a promising strategy for food waste disposal.Keywords:butanol production;food waste;direct fermentation;no nutrient supplementation;Clostridium saccharoperbutylacetonicum
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NEW JOURNAL OF CHEMISTRY
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2023
·Vol. 47
·期 17
·8161-8169
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IF 3.3
Shangjin Liu,Chunfeng Mao,Chenghan Yang,Pengda Hong,Min Zhu,Yuming Zhou,Yiwei Zhang
4-Nitrophenol (4-NP) usually exists in soil and groundwater, which causes great harm to the environment and human health. Removal of 4-nitrophenol has become the focus of research. Herein, a smart synthetic strategy of NH2-MIL-88B(Fe)@Au@Covalent Organic Frameworks (COFs) is proposed for the first time. NH2-MIL-88B(Fe)@Au@COFs are composed of NH2-MIL-88B(Fe) as the core and COFs as the shell with Au nanoparticles. Au nanoparticles in NH2-MIL-88B(Fe)@Au@COFs exhibit good dispersion and thermal stability due to amino group immobilization on NH2-MIL-88B(Fe). The core–shell structure, high thermal stability and highly dispersed active sites of NH2-MIL-88B(Fe)@Au@COFs were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), thermal gravimetric analysis (TGA), and so on. To the best of our knowledge, 4-nitrophenol was reduced to 4-aminophenol using NH2-MIL-88B(Fe)@Au@COFs as the catalyst, NaBH4 as the reductant and 4-nitrophenol as the raw material at room temperature. The catalyst has high catalytic activity and can be used repeatedly for the reduction of 4-nitrophenol under the cooperation of Fe and Au electrons. In addition, the kinetics and mechanism of catalytic reduction were discussed in detail. Multi-functional materials constructed using metal organic frameworks (MOFs) and COFs provide a basis for the application and expansion of new porous materials.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 15
·4808-4818
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IF 4
Xiujuan Zhao,Yanmin Wang,Zhidong Pan,Youjun Lu,Junhao Li,Mingmei Wu
A Sm3+-doped YGa1.5Al1.5(BO3)4 (abbreviated as YGAB) phosphor was synthesized via a solid-state reaction with mechanical activation assistance in a high-energy density stirred bead mill. The samples were characterized by laser particle size analysis, specific surface area analysis, powder X-ray diffraction (XRD), scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and fluorescence spectroscopy. In addition, the photoluminescence characteristics, luminescence decay, thermal stability, and LED application of the phosphors were also investigated. The results show that the mechanical activation of mixed raw materials before calcination can reduce the particle size and the activation energy of crystallization, resulting in the formation of the YGAB crystal phase at a lower calcination temperature. The mechanical activation in grinding can accelerate the subsequent solid-phase reaction, make Sm3+ ions more easily diffuse into the YGAB lattice, and improve the crystal structure of the synthesized phosphor, thus enhancing the optical properties of the phosphor. According to the photoluminescence emission (PL) and excitation (PLE) spectra, Sm3+ doped in the YGAB lattice can provide an efficient emission under 405 nm excitation. The optimum doping concentration of Sm3+ ions is 0.03 mol%. The optimum photoluminescence intensity, quantum yield, and fluorescence lifetime of the phosphor synthesized from mixed raw materials ground for 45 min can be obtained. The temperature-dependent PL spectra show that the emission intensity of the YGAB:0.03Sm3+ phosphor at 425 K is 84.7% of its initial intensity at room temperature. It is indicated that the YGAB:0.03Sm3+ phosphor synthesized could be used as one of the promising LED lighting materials.
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AQUATIC TOXICOLOGY
·
2023
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IF 4.5
Yitong Lu,Xinxin Jiang,Hongzhou Xu,Chengrong Liu,Yanzhen Song,Kuiquan Pan,Lixin Wang,Liang Du,Haixia Liu
Global warming has increased the frequency of Microcystis aeruginosa blooms, leading to the deterioration of water quality and loss of biodiversity. Therefore, developing effective strategies for controlling M. aeruginosa blooms has become an important research topic. Plant extracts, 4‑tert-butylpyrocatechol (TBC) and tea polyphenol (TP) are commonly used for water purification and to increase fish immunity, which have great potential to inhibit cyanobacterial blooms. The inhibitory effects of TBC and TP on M. aeruginosa were investigated in terms of growth characteristics, cell membrane morphology, physiological, photosynthetic activities, and antioxidant enzymes activities . The results showed that TBC and TP inhibited the growth of M. aeruginosa by decreasing the chlorophyll fluorescence transients or increasing the antioxidant enzymes activities of M. aeruginosa . TBC damaged the cell morphology of M. aeruginosa , reduced extracellular polysaccharides and protein contents, and up-regulated the antioxidant activity-related gene ( sod and gsh ) expressions of M. aeruginosa . TP significantly decreased the photosynthetic pigment content, influenced the phycobiliprotein content, and strongly down-regulated the photosynthesis-related gene ( psbA, psaB, and rbcL ) relative expressions of M. aeruginosa . TBC caused significant oxidative stress , dysfunction of physiological metabolic processes, and damaged crucial biomacromolecules (e.g., lipids, proteins and polysaccharides), prompted the loss of cell integrity, ultimately leading to the death of M. aeruginosa . However, TP depressed photosynthetic activities and consequently inhibited the transfer of electrons, affected the electron transfer chain , decreased the photosynthetic efficiency , and eventually caused the death of M. aeruginosa cells. Our study showed the inhibitory effects and algicidal mechanisms of TBC and TP on M. aeruginosa , and provide a theoretical basis for restrain the overgrowth of M. aeruginosa .
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2019
·Vol. 33
·期 5
·e4865
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IF 3.259
Chao Duan,Chaoran Liu,Xin Meng,Wanli Lu,Yonghao Ni
In this work, we prepared high-performance and recyclable nanocatalysts that consist of small and well-dispersed silver nanoparticles (Ag NPs) immobilized onto Cu- based metal–organic framework (MOF-199 s) supported by carboxymethylated cellulose fibers (CCFs). The as-prepared green nanohybrid catalysts, namely Ag NPs@ MOF-199 s/CCFs, were characterized using SEM, TEM, XRD and FT-IR techniques. The catalytic performances showed that Ag NPs@ MOF-199 s/CCFs catalysts exhibited a very high catalytic efficiency towards the reduction of 4-nitrophenol to 4-aminophenol. The enhanced catalytic performances are attributed to the improved dispersity, small particles of Ag NPs stabilized by the MOF-199 s, and the porous catalyst structures. The introduction of cellulose fiber further facilitates the reuse and sustainability of the nanohybrid catalysts, showing a stable and high reusability (more than 91% of catalytic activity) even after five runs.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2018
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IF 2.559
Fan Zhang,Chenhong Zhang
Intracerebral hemorrhage (ICH) is reported as a common and often fatal type of stroke accompanied with high morbidity and mortality, and it frequently results in long-lasting neurological dysfunctions. However, the pathogenesis that contributes to ICH has not been fully understood. Rnf112, also known as Znf179, is a member of the RING finger protein family. The expression of Rnf112 is abundant in the brain and is modulated during brain progression and development. The study aimed to explore the role of Rnf112 in brain injury after ICH, as well as the underlying molecular mechanisms. The results indicated that ICH led to a significant decrease in Rnf112, which was confirmed in oxyhemoglobin (oxyHb)-incubated astrocytes and microglial cells. Moreover, the Rnf112 knockout (Rnf112 −/− ) mice and wild type (WT) mice induced by ICH were further employed. Compared to the WT/ICH group, Rnf112 −/− mice exhibited accelerated brain injury, as evidenced by the increased brain water contents and neurological deficit scores (NDS). In comparison to WT/ICH group, a remarkable up-regulation in the release of pro-inflammatory cytokines, including tumor necrotic factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β, was observed in perihematoma tissues of Rnf112 −/− mice on day 3 post-ICH. The process was along with promoted glial fibrillary acidic protein (GFAP) and Iba1 expression and reduced NeuN levels. Furthermore, ICH-induced increases in toll-like receptor (TLR)-4 and myeloid differentiation primary response protein (MyD88) expression were exacerbated by the loss of Rnf112. The phosphorylated expression of IKKα, inhibitor of NF-κB (IκBα) and nuclear factor-kappa B (NF-κB) induced by ICH in perihematoma tissues of mice was markedly enhanced in Rnf112 −/− mice. Rnf112 repression-induced inflammatory response was verified in lipopolysaccharide (LPS)-incubated glial cells . In contrast, over-expressing Rnf112 markedly attenuated ICH-induced brain injury by restraining inflammation via inactivating TLR-4/NF-κB pathway. In summary, our findings suggested that Rnf112 expression was highly involved in the progression of ICH, and targeting Rnf112 signaling might be a promising therapeutic strategy against ICH development.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Hejie Qin,Daqiang Yin,Joel Z. Bandstra,Yuankui Sun,Guomin Cao,Xiaohong Guan
In this study, Fe 2+ addition was employed to overcome the negative effects of humic acid (HA) on contaminant removal by zerovalent iron (ZVI), and its feasibility to improve electron efficiency of ZVI was also tested. HA at high concentrations suppressed the removal of 4-nitrophenol (4-NP) by ZVI, while the addition of 0.25–1.0 mM Fe 2+ could greatly mitigate this inhibitory effect and enhance 4-NP reduction. Specifically, with a mixed-order model, global fitting results showed that the addition of Fe 2+ increased the rate constant from 0.124 × 10 −2 –0.219 × 10 −2 mM/min to 0.227 × 10 −2 –0.417 × 10 −2 mM/min and shortened lag period from 19.7–47.9 min to 8.0–15.2 min for 4-NP removal. The mechanistic investigation revealed this trend could be explained by the following aspects: i ) Fe 2+ can facilitate the generation of Fe(II)-containing oxides, which can act as an electron mediator or direct electron donor for 4-NP reduction; ii ) the presence of Fe 2+ could lead to aggregation of HA particles and accordingly reduced its coverage on ZVI surface. But the results of respike experiments indicate that Fe 2+ addition did not show remarkable effect on the electron efficiency of 4-NP by ZVI, which should be associated with that Fe 2+ was not able to favor the enrichment of 4-NP on ZVI surface.
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危险品化学品经营许可证（带存储）