JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Wan Wang,Hang Shang,Jinzhe Li,Yue Ma,Cong Xu,Jiage Ma,Juncai Hou,Zhanmei Jiang
Polyphenols have received attention as dietary supplements for the relief of alcoholic liver disease (ALD) due to various bioactivities. Ethanol-induced rat small intestinal epithelial cell 6 (IEC-6) and alpha mouse liver 12 (AML-12) cell models were pretreated with four dietary polyphenols with different structures to explore their effects on cytotoxicity and potential protective mechanisms. The results showed that polyphenols had potential functions to inhibit ethanol-induced AML-12 and IEC-6 cell damage and oxidative stress, and restore ethanol-induced IEC-6 permeability and tight junction gene expression. Especially, dihydromyricetin (DMY) had the best protective effect on ethanol-induced cytotoxicity, followed by apigenin (API). Western blot results showed that DMY and API had the best ability to inhibit CYP2E1 and Keap1, and promote nuclear translocation of Nrf2, which might be the potential mechanism by which DMY and API attenuate ethanol-induced cytotoxicity. Moreover, the molecular docking results predicted that DMY and API could bind more tightly to the amino acid residues of CYP2E1 and Keap1, which might be one of the inhibitory modes of dietary polyphenols on CYP2E1 and Keap1. This study provided a rationale for the subsequent protective effect of dietary polyphenols on alcohol-induced liver injury in animal models and provided new clues on bioactive components for ALD-protection based on the gut-liver axis.
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Small Methods
·
2022
·Vol. 6
·期 8
·2200404
·
IF 15.367
Chun-Mei Xu,Jian Peng,Xiao-Hao Liu,Wei-Hong Lai,Xiang-Xi He,Zhuo Yang,Jia-Zhao Wang,Yun Qiao,Li Li,Shu-Lei Chou
Prussian blue analogues (PBAs) have attracted extensive attention as cathode materials in sodium-ion batteries (SIBs) due to their low cost, high theoretical capacity, and facile synthesis process. However, it is of great challenge to control the crystal vacancies and interstitial water formed during the aqueous co-precipitation method, which are also the key factors in determining the electrochemical performance. Herein, an antioxidant and chelating agent co-assisted non-aqueous ball-milling method to generate highly-crystallized Na 2- x Fe[Fe(CN) 6 ] y with hollow structure is proposed by suppressing the speed and space of crystal growth. The as-prepared Na 2- x Fe[Fe(CN) 6 ] y hollow nanospheres show low vacancies and interstitial water content, leading to a high sodium content. As a result, the Na-rich Na 1.51 Fe[Fe(CN) 6 ] 0.87 ·1.83H 2 O hollow nanospheres exhibit a high initial Coulombic efficiency, excellent cycling stability, and rate performance via a highly reversible two-phase transition reaction confirmed by in situ X-ray diffraction. It delivers a specific capacity of 124.2 mAh g −1 at 17 mA g −1 , presenting ultra-high rate capability (84.1 mAh g −1 at 3400 mA g −1 ) and cycling stability (65.3% capacity retention after 1000 cycles at 170 mA g −1 ). Furthermore, the as-reported non-aqueous ball-milling method could be regarded as a promising method for the scalable production of PBAs as cathode materials for high-performance SIBs.
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Neural Regeneration Research
·
2021
·
IF 5.135
Ling-Yu Zhang,Qian-Qian Jin,Christian Hölscher,Lin Li
Patients with Parkinson’s disease (PD) have impaired insulin signaling in the brain. Incretin hormones, including glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), can re-sensitize insulin signaling. In a recent phase II clinical trial, the first GLP-1 mimic, exendin-4, has shown reliable curative effect in patients with PD. DA-CH5 is a novel GLP-1/GIP receptor unimolecular co-agonist with a novel peptide sequence added to cross the blood-brain barrier. Here we showed that both exendin-4 and DA-CH5 protected against 6-hydroxydopamine (6-OHDA) cytotoxicity, inhibited apoptosis, improved mitogenesis and induced autophagy flux in SH-SY5Y cells via activation of the insulin receptor substrate-1 (IRS-1)/alpha serine/threonine-protein kinase (Akt)/cAMP response element-binding protein (CREB) pathway. We also found that DA-CH5 (10 nmol/kg) daily intraperitoneal administration for 30 days post-lesion alleviated motor dysfunction in rats and prevented stereotactic unilateral administration of 6-OHDA induced dopaminergic neurons loss in the substantia nigra pars compacta. However, DA-CH5 showed curative effects in reducing the levels of α-synuclein and the levels of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-1β). It was also more effective than exendin-4 in inhibiting apoptotic process and protecting mitochondrial functions. In addition, insulin resistance was largely alleviated and the expression of autophagy-related proteins was up-regulated in PD model rats after DA-CH5 treatment. These results in this study indicate DA-CH5 plays a therapeutic role in the 6-OHDA-unilaterally lesioned PD rat model and is superior to GLP-1 analogue exendin-4. The study was approved by the Animal Ethics Committee of Shanxi Medical University of China.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
He Li,Xiang-Yi Tang,Chun-Jian Wu,Shu-Juan Yu
The 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) is one of the Mallard reaction product with intense bitter and strong antioxidant activity . Insight into DDMP formation pathways is important to manage Maillard reaction products quality. In this work, wet- and dry-heating glucose-amino acid model reactions were investigated to understand the DDMP formation pathways. Lysine and proline facilitated the DDMP generation in wet- and dry-heating system, respectively, suggesting the reaction intensity and amino acid properties are primary factors mediating DDMP generation. Fundamental mechanism on DDMP formation or inhibition was evaluated via quantitatively monitoring enolization products, which suggested that the formation of DDMP was facilitated at lower moisture content condition caused by more 1-deoxyglucosone (1-DG) generation via 2,3-enolization. However, increasing moisture content resulted in the decay of DDMP because of the transformation of 2,3-enolization to 1,2-enolization, which inhibited DDMP generation but transformed to form 5-hydroxymethylfurfural (HMF).
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
He Li,Chun-Jian Wu,Xiang-Yi Tang,Shu-Juan Yu
The control of 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one (DDMP) formation in the Maillard reaction is important to improve the thermally treated food quality as a result of its intense bitterness and potential toxicity. In this work, phenolic acids, such as gallic, protocatechuic, caffeic, and ferulic acids, were applied to modulate DDMP formation in a microaqueous glucose–proline model. The formation of DDMP was inhibited at low concentrations (from 0.1 to 5.0 mM) while enhanced at 10.0 mM gallic, protocatechuic, and caffeic acids. Ferulic acid always inhibited DDMP formation as a result of the absence of catechol groups on its benzene ring. The result indicated that the control of DDMP formation depended upon the concentration and chemical structures of phenolic acids, such as the number of hydroxyl groups. Further studies indicated that the hydroxyl distribution of phenolic acids regulated the peroxide formation in the model reaction system and further changed the development of the oxidation reaction, which affected the degradation of glucose via caramel or Maillard reaction, Amadori rearrangement product oxidation, and 1-deoxyglucosone degradation to form the intermediates.
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2013
·Vol. 41
·期 5
·3243-3260
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IF 0.88
Fa HuanBao,Zhou JingTing,Zhang Dong,Yin Wei,Zhang HaiFeng,Huo DanQun,Hou ChangJun,Luo XiaoGang,Mao YaLi,Zhang Jin
A novel 4-(2-{[6-(1,1-dicyanoprop-1-en-2-yl)naphthalen-2-yl](methyl)amino} ethoxy)-4-oxobutanoic acid ( 5 ) fluorescent probe for β-amyloids was synthesized by catalytic acylation using 4-dimethylaminopyridine between succinic anhydride and (1-{6-[(2-hydroxyethyl)(methyl) amino]-2-naphthyl}ethylidene)malononitrile ( 4 ). The structures of all compounds were identified by proton nuclear magnetic resonance spectroscopy, infrared spectroscopy, mass spectrometry, and ultraviolet–visible (UV–Vis) spectroscopy. The UV–Vis and fluorescence spectra of 1-{6-[(2-hydroxyethyl)(methyl) amino]-2-naphthyl}ethan-1-one ( 3 ), 4 , and 5 in solvents with different polarities were investigated, and the effects of solvent polarity on the optical properties of the three compounds were studied. The objective product 5 showed high binding affinities toward Aβ(1–40) aggregates in vitro ( K
d = 29.4 nmol/L) by fluorophotometry. This study provides a powerful fluorescent probe for the molecular diagnosis of Alzheimer’s disease.
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RSC Advances
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2016
·Vol. 6
·期 114
·113209-113218
·
IF 3.289
Jun Yu,Feng Xiao,James Asenso,Peng Zhou,Xiao-Dan Yang,Chun Wang,Wei Wei
Paeoniflorin (Pae), a water-soluble monoterpene glucoside, is the main effective component of total glucoside of paeony (TGP). Pae has a very low bioavailability (3.6%). The aim of this study was to investigate the effects of sex, food and disease status on the pharmacokinetics of paeoniflorin-6′-O-benzene sulfonate (acylated derivative of Pae, CP-25) and its primary metabolite (Pae, M1) after oral administration in the plasma of rats. A specific and sensitive ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) assay for rapid determination of CP-25 and Pae in rat plasma was developed and validated for the first time. The assay was validated in terms of selectivity, linearity, accuracy, precision, recovery, matrix effects and stability. We found that CP-25 had a more extensive distribution in females than that in males, but no differences in M1 levels were found. Food intake could also increase the extent of absorption and decrease the rate of clearance for CP-25 and M1 after oral administration in rats. The disease status would decrease the absorption of CP-25 in rats. Comparing single-doing and multiple-dosing in an adjuvant arthritis (AA) model, the absorption of CP-25 and M1 improved with increasing dosage. Furthermore, during the period of multiple-dosing, CP-25 exhibited a better anti-inflammatory effect in AA rats and no obvious side effects occurred during the dosing period. Our findings provide the basis, and some guidelines, for further studies into the clinical application of CP-25.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Lan Li,Yaqi Yan,Yue Tang,Binbin Lu,Jiayu Wang,Yixiang Duan
Caffeine is an alkaloid compound that can excite the central nervous system and effectively relieve fatigue in moderation. However, excessive caffeine consumption can block blood vessels, raising blood pressure and increasing the risk of atherosclerosis. The International Agency for Research on Cancer of the World Health Organization has listed caffeine in category 3 carcinogens, so it is crucial that caffeine be detected easily and accurately. Exhaled breath aerosol (EBA), which consists of a large number of volatile substances and complex biological matrices, can not only reflect the physiological state of the human body, ingested substances, and environmental exposures; on the other hand, the characteristics of non-invasive sampling also make EBA analysis has a broad application prospect in the field of human health monitoring and disease diagnosis. This paper used a combination of nylon 6 film coated with poly-tetrahydrofuran (PTHF) and a medical N95 mask to construct a convenient and direct sampling device, which can be used to collect caffeine ingested by humans non-invasively from EBA. Volunteers wore a mask for a defined period of time after consuming a certain amount of caffeine. The samples were next back-extracted with methanol and detected and analyzed using gas chromatography-high resolution mass spectrometry (GC-HRMS). The results showed that under the optimized analytical conditions, the PTHF-coated nylon 6 film was effective in collecting caffeine from human EBA, and the peak time of caffeine was 23.44 min. The mask-loaded coated device in this study has the advantages of being comfortable and efficient, safe and non-invasive as well as easy to operate, and by combining with mass spectrometry molecular techniques it can achieve high sensitivity and specificity analysis of trace components, which is expected to be used for the early diagnosis of respiratory diseases in clinical analysis.
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Genetic Testing and Molecular Biomarkers
·
2016
·
IF 1.297
Chunze Zhang,Yijia Wang,Wenzheng Fu,Weihua Zhang,Tao Wang,Hai Qin
Objectives: We aimed to investigate the influence of ulinastatin (UTI) on the serum levels of C-reactive protein (CRP), interleukin 6 (IL-6), and tumor necrosis factor alpha (TNF-α) in Asian patients with acute pancreatitis (AP) by performance of a meta-analysis. Methods: Two investigators independently searched 11 databases, including PUBMED, EBSCO, Ovid, SpringerLink, Wiley, Web of Science, Cochrane Library, Wanfang database, China National Knowledge Infrastructure (CNKI), Chinese Journal Full-text Database, and China Biomedicine Database. The full-text articles were screened and the data were extracted using a standardized data extraction form. All statistical analyses were conducted with Stata software, version 12.0 (Stata Corporation, College Station, TX). Results: A total of 94 studies were initially retrieved, and 10 studies containing 424 Asian patients with AP were ultimately enrolled in this meta-analysis. The results revealed that the serum levels of CRP, IL-6, and TNF-α in Asian AP patients significantly decreased after UTI therapy (CRP: standardized mean difference [SMD] = 3.26, 95% confidence interval [CI] = 1.69–4.83, p < 0.001; IL-6: SMD = 5.92, 95% CI = 2.09–9.75, p = 0.002; TNF-α: SMD = 4.07, 95% CI = 0.79–7.35, p = 0.015). Conclusion: The results of this meta-analysis suggest that UTI can effectively depress the serum levels of CRP, IL-6, and TNF-α in Asian patients with AP, and thereby inhibit inflammation.
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JOURNAL OF POLYMER ENGINEERING
·
2023
·Vol. 43
·期 8
·759-769
·
IF 2
Baomin Fu,Longlong Yang,Wenwen Xiang,Xiaoxuan Zou,Wenli Dai
It is a challenge to maintain the mechanical properties and processability of thermally conductive polymer composites in the presence of high filling of heat conductive filler. An optimized hybrid filler system composed of flake graphite (FG), expanded graphite (EG) and short carbon fiber (CF) was introduced into PA6 matrix. The addition of EG to PA6 was found to be more effective in improving its thermal conductivity, while the addition of FG maintained favorable processability due to its lubrication effect. Furthermore, the hybrid filling of FG and EG has a synergistic effect on the enhancement of thermal conductivity. The ternary filling of FG, EG and CF produced highly heat conductive PA6 composites with high strength, favorable processability, and low water absorption. The thermal conductivity of 10CF/20FG/10EG/PA6 composite reached 3.45 W/m k, which is 12.3 times of pure PA6. Additionally, the flexural strength increased to 110 MPa, which is 37 % higher than that of pure PA6, and the water absorption was reduced to one quarter that of pure PA6.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2026
·
IF 3.4
Shuang Feng,Yue Deng,Jiarui Zhang,Jiale Liu,Xinyue Zhou,Yalin Xi,Lei Yin,Meiyun Shi
Short-chain polyethylene glycol derivatives, such as methoxy polyethylene glycol with 6 subunits (M‑PEG₆‑OH), are extensively utilized excipients and building blocks, yet their in vivo biodistribution and associated safety profiles remain insufficiently characterized. To address this knowledge gap, this study developed a novel, environmentally conscious analytical platform based on ultra‑performance convergence chromatography‑tandem mass spectrometry (UPC²‑MS³) for the sensitive quantification of M‑PEG₆‑OH in zebrafish tissues. A key methodological innovation involved the selective formation and detection of ammonium adducts ([M+NH₄]⁺) as precursor ions, coupled with a post‑column infusion strategy. This combined approach effectively mitigated ion suppression and significantly enhanced ionization efficiency, yielding an average 5‑fold increase in analytical signal response. The fully validated method achieved rapid and baseline separation of the analyte within 1.5 min. Its successful application to a tissue distribution study revealed distinct, time‑dependent accumulation profiles, with the highest levels observed in gill, followed by liver and muscle tissues. Furthermore, the methodology was rigorously evaluated using established metric systems, demonstrating excellent environmental sustainability (AGREE score = 0.62) and outstanding practical applicability (BAGI score = 72.5). This work provides crucial insights into the tissue‑specific disposition of short‑chain PEG derivatives and establishes a sustainable, high‑performance analytical framework for the safety evaluation of PEGylated compounds.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Meng Wang,Jingjing Wang,Kangkang Wang,Liugen Zhang,Xianglei Cao,Cheng Guo,Jide Wang,Bin Wu
More and more attention has been paid to the improved QuEChERS (quick, easy, cheap, effective, rugged and safe) method in dealing complex sample matrices, especially for the study of QuEChERS adsorbents. In this study, a magnetic mesoporous material, which was derived from MIL-88B modified by l -alanine, was synthesized as modified QuEChERS adsorbents for the simultaneous determination of multiple pesticides (Methomyl, Isoprocarb, Carbofuran, 3-Hydroxycarbofuran, Acetamiprid, Imidacloprid) in Chinese cabbage, celery, long bean and leek. The prepared magnetic adsorbents can effectively remove interfering substances from the sample, and the proposed modified QuEChERS method can reduce sample pretreatment time via an external magnetic field. To achieve the best performance of QuEChERS method, the clean-up time and amount of QuEChERS adsorbents were investigated. Under the optimized conditions, a simple, rapid and sensitive method for the determination of 6 pesticide residues in vegetables was established by coupling the modified QuEChERS to ultrahigh-performance liquid chromatography-tandem mass spectrometry. Excellent sensitivity (The limit of detection for the 6 pesticides ranged from 0.001 to 0.020 µg kg −1 ), satisfactory linearity (r 2 ≥ 0.9952), good recovery (73.9–107.7%) and good precision (3.6–16.9% for intraday relative standard deviation, 0.5–15.0% for interday relative standard deviation) were obtained. Compared with traditional QuEChERS method, the proposed method is simple, cost-effective, and efficient, which indicates that the method can be used to detect carbamate and neonicotinoid pesticides in real samples and provide an excellent pretreatment technique for the detection of trace multi-analytes from complex substrates.
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危险品化学品经营许可证（带存储）