CHEMOSPHERE
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2018
·
IF 4.427
Xue Xue Liang,A.M. Omer,Zhao-hong Hu,Yang–guang Wang,Di Yu,Xiao-kun Ouyang
In this study, we prepared a magnetic composite based on amine-functionalized chitosan (aminochitosan; AmCS) and Fe 3 O 4 to remove diclofenac sodium (DS) from water. The fabricated AmCS@Fe 3 O 4 composite was characterized using Fourier-transform infrared spectroscopy, transmission electron microscopy , scanning electron microscopy, vibrating sample magnetometry, X-ray diffraction, and thermogravimetric analysis . Furthermore, we investigated the influence of pH, initial DS concentration, and adsorbent dosage on the adsorption of DS. Through thermodynamic analysis, we found that the data corresponded with the Langmuir adsorption isotherm model. The maximum adsorption capacity reached 469.48 mg g −1 , and the adsorption process followed the pseudo-second-order kinetic model. Finally, the AmCS@Fe 3 O 4 composite retained good adsorption characteristics after four consecutive cycles, with removal efficiency exceeding 70%. Therefore, the developed adsorbent could be used for efficient adsorptive removal of trace drugs and personal care products from water bodies.
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PubMed
高影响因子
MOLECULES
·
2018
·Vol. 23
·期 10
·2611
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IF 3.098
Xionglin Chen,Xiaoming Cao,He Jiang,Xiangxin Che,Xiaoyuan Xu,Baicheng Ma,Jie Zhang,Tao Huang
Skin wound healing is a complex and dynamic process that involves angiogenesis and growth factor secretion. Newly formed vessels can provide nutrition and oxygen for skin wound healing. Growth factors in skin wounds are important for keratinocytes and fibroblasts proliferation, epithelialization, extracellular matrix remodeling, and angiogenesis, which accelerate skin wound healing. Therefore, treatment strategies that enhance angiogenesis and growth factors secretion in skin wounds can accelerate skin wound healing. This study investigated the effects of a SIKVAV (Ser-Ile-Lys-Val-Ala-Val) peptide-modified chitosan hydrogel on skin wound healing. Hematoxylin and eosin (H&E) staining demonstrated that the SIKVAV-modified chitosan hydrogel accelerated the re-epithelialization of wounds compared with that seen in the negative and positive controls. Masson’s trichrome staining showed that more collagen fibers were deposited in the skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. Immunohistochemistry assays demonstrated that more myofibroblasts were deposited and more angiogenesis occurred in skin wounds treated with the SIKVAV-modified chitosan hydrogel than in the negative and positive controls. In addition, ELISA assays showed that the SIKVAV-modified chitosan hydrogels promoted the secretion of growth factors in skin wounds. Taken together, these results suggest that the SIKVAV-modified chitosan hydrogel has the potential to be developed as synthesized biomaterials for the treatment of skin wounds.Keywords:SIKVAV;chitosan hydrogel;skin wound healing;angiogenesis;growth factor
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CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2013
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IF 0.769
Yuan-Yuan JIANG,Kun WANG,Chong-Zheng XU,Xiao-Di YANG,Hui-Hui LI
In this work, the glassy carbon electrode modified by alizarin (AR) hybridized with graphene (CRG) and chitosan (CS) was prepared. The AR/CRG-CS electrode exhibited a pair of well-defined reversible redox peaks in 0.1 M phosphate buffer solution (PBS, pH 7.4) with the oxidation peak at −0.573 V and the reduction peak at −0.652 V. It exhibited a two-proton and two-electron redox process and a fast heterogeneous electron transfer rate with the rate constant ( K s ) of 1.69 s −1 using the cyclic voltammetry. Based on the characteristics of alizarin with the decreased current and negative potential shift after binding with human telomere DNA, a sensitive, simple, reliable and stable electrochemical method for the detection of human telomere DNA using the AR/CRG-CS electrode was developed. The electrochemical response showed a linear relationship with DNA concentration in the range of 8.0 × 10 −8 −1.4 × 10 −5 M ( R = 0.9995) with the linear regression equation as Δ I pc (μA) = 8.4860 + 0.5366 C (μmol) and the detection limit ( S/N ) as 2.0 × 10 −8 M.
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2013
·Vol. 102
·期 3
·801-807
·
IF 2.834
Ke Jiang,Ziming Wang,Quanyin Du,Jiang Yu,Aimin Wang,Yan Xiong
The post-operative outcome of flexor tendon healing remains limited by flexor tendon adhesion that reduces joint range of motion. Despite improvement in different methods, peritendinous adhesion formation continues to present a formidable challenge. Recent studies showed that transforming growth factor-β3 (TGF-β3) may be the key factor to reducing adhesion formation in skin or tendon. In this study, we designed a novel type of tissue engineering synovial sheath containing TGF-β3, to prevent flexor tendon adhesion. First, to achieve a stable release of TGF-β3, chitosan microspheres, prepared by crosslinking-emulsion, were used for the delivery of TGF-β3. Second, a three-dimensional chitosan scaffold was prepared by lyophilization, and TGF-β3 microspheres were carefully introduced into the scaffold. Then, synovial cells were cultured and then seeded into the TGF-β3 loaded scaffold to produce TGF-β3 controlled-released tissue engineering synovial sheath. Tests clearly demonstrated that the scaffold has good structure and compatibility with cells. These results expand the feasibility of combinative strategies of controlled protein release and tissue-engineered synovial sheath formation. Application of this scaffold to tendon repair sites may help to prevent adhesion of tendon healing. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 801–807, 2014.
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CHEMICAL PAPERS
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2012
·Vol. 67
·期 4
·423-428
·
IF 1.096
Zhu Guo-Quan,Wang Fa-Gang,Liu Yu-Ying,Gao Qiao-Chun
A series of water-soluble carboxymethyl chitosan (CMCS)/poly(propylene glycol) (PPG) blend films with various CMCS/PPG mole ratios were prepared by the solution casting method. Morphology of the CMCS/PPG blend films was investigated by scanning electron microscopy (SEM). Thermal, mechanical, and chemical properties of the CMCS/PPG blend films were studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), tensile tests, and contact angle tests. It was found that the introduction of PPG can markedly affect the morphology and the properties of CMCS films.
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POLYMER DEGRADATION AND STABILITY
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2012
·
IF 2.769
Ying Wan,Junchao Huang,Jun Zhang,Dengke Yin,Zijie Zheng,Chunyan Liao,Shaofa Sun
Multi-channel chitosan–polycaprolactone (CH–PCL)/collagen conduits were fabricated for potential applications in long-gap peripheral nerve repair. CH–PCLs with various PCL percentages changing from around 30 to 45 wt% were used for the fabrication of conduits, and the collagen content in the conduits was controlled at about 8 wt% or less in order to ensure the required mechanical strength and degradation rate of the resulting conduits. The porosity and average channel diameter of the conduits with a dimension of around 5 mm in outer diameter and 30 mm in length were optimized as around 80% and 200 μm, respectively. Swelling index, compressive load, deformation recovery and bending stiffness of the conduits were measured, respectively. In vitro degradation measurements was conducted in phosphate buffer saline, and results revealed that the effect of collagen content on the degradation rate of the conduits was not significant until the degradation time reached around 6 weeks. After being implanted into rabbits for various durations, the degradation of the conduits appeared to be strongly dependent on the collagen content in addition to the dependence on the degradation time. After 10-week in vivo degradation, some conduits that contained 6 wt% or less content of collagen still showed shape integrity and required compressive mechanical properties , suggesting that the degradation rate of the conduits can be effectively regulated by the collagen content while the required properties for the conduits would be well maintained.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2014
·
IF 3.552
Hao Guo,Dongying Yuan,Guoqi Fu
Surface protein imprinting over nano- or micron-sized substrates is an effective approach for improving the biomacromolecule mass transfer and rebinding capacity. For achieving high recognition performance, it is necessary to introduce certain functional groups onto the surface of the support materials which can interact with the template protein. Herein, we report a surface protein imprinting approach using a new kind of core–shell magnetic chitosan submicrospheres as the supports. The surface of these magnetic chitosan particles is tethered with uncross-linked chitosan chains, hence bearing plenty of amino and hydroxyl groups, where a large amount of functional ligands can be readily coupled for capturing of the protein template. With lysozyme as a model print protein, the magnetic supports were functionalized with maleic acid and then coated with imprinted polymer layers. The resulting imprinted microspheres show significantly selective rebinding for lysozyme. In particular, they exhibit a specific rebinding capacity about three times higher than achieved with our previous lysozyme-imprinted particles synthesized in similar way but with maleic acid modified silica nanoparticles as the supports. This can be attributed to the much higher template binding capacity to the modified magnetic chitosan submicrospheres. Also, the resultant imprinted particles can be easily collected by a magnet. Therefore, such kind of chitosan submicrospheres may be a versatile carrier for constructing high-capacity and magnetically recyclable surface protein-imprinted particles.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 132
·期 32
·
IF 1.768
Zhengyan Tan,Hong Peng,Hongfang Liu,Linling Wang,Jing Chen,Xiaohua Lu
A novel magnetic adsorbent (EDTA /chitosan/ PMMS) was facilely prepared by reacting chitosan with EDTA anhydride in presence of PEI - coated magnetic microspheres. The as-synthesized EDTA/ chitosan /PMMS was characterized by XRD, SEM, TGA, FT-IR , and VSM, and then employed in removal of heavy metals of Pb(II) from aqueous solution. The results of the batch adsorption experiments revealed that the adsorbents had extremely high uptake capacities for Pb(II) in the pH range of 2 to 5.5, and the adsorption kinetics for EDTA/ chitosan /PMMS was consistent with the pseudo – second - order kinetic model. Moreover, its equilibrium data were fitted with the Langmuir isothermal model well, which indicated that the adsorption mechanism was a homogeneous monolayer chemisorptions process. The maximum adsorption capacity of EDTA/ chitosan /PMMS for Pb(II) was found to be 210 mg g − 1 at pH 4 (30 ° C), and further reuse experiments results suggested that EDTA /chitosan/ PMMS could be a potential recyclable magnetic adsorbent in the practical wastewater treatment. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 42384.
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CARBOHYDRATE POLYMERS
·
2014
·
IF 3.916
Wenjiao Ge,Dong Li,Meiwan Chen,Xiaohui Wang,Shijie Liu,Runcang Sun
β-Carotene (β-C) is a well-established natural antioxidant agent, but its poor water-solubility, low chemical stability and low bioavailability limit its application in food, pharmaceuticals and cosmetics industries. Thus it is critical to develop an efficient method to improve the water solubility and stability of β-C. In this research, amphiphilic chitosan-graft-poly (lactide) (CS-g-PLA) copolymer was synthesized via a homogeneous ring-opening polymerization (ROP) in ionic liquid. The obtained CS-g-PLA copolymer was able to self-assemble into about 14 nm micelles in water at low concentration, and β-C loaded micelles (β-C/M) had low β-C degradation after 15 days. The antioxidant properties of the β-C/M were investigated by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method and ferric reducing antioxidant power (FRAP) method, respectively. Significantly improved antioxidant activity was observed for β-C/M in compare with free β-C. The aqueous dispersion of β-C/M has potential to be applied in the field of functional food and cosmetics.
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SURFACE & COATINGS TECHNOLOGY
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2014
·
IF 2.199
Linlin Huang,Jinhong Yi,Qiang Gao,Xudong Wang,Yashao Chen,Peng Liu
To improve the corrosion resistance and cytocompatibility of magnesium alloy (Mg alloy), an anticorrosive and bioactive composite coating was triumphantly prepared on Mg alloy surface. First, Mg alloy substrates were treated by cathodic plasma electrolytic deposition (CPED). Then, bioactive carboxymethyl chitosan (CMCS) was covalently immobilized on CPED-treated Mg alloy via polydopamine as intermediate layer. X-ray photoelectron spectroscopy and scanning electronic microscopy were employed to detect the change of chemical composition and surface morphology of Mg alloy substrate at various stages of surface modification. Results of water contact angle measurement showed that the hydrophilicity of the surface has improved significantly after surface modification. Potentiodynamic polarization data and hydrogen evolution test demonstrated that corrosion resistance of modified Mg alloy significantly increased compared with that of pristine sample. Cytotoxicity test and cell morphology analysis confirmed that adhesion and proliferation of osteoblasts on CMCS-modified Mg alloy surface were improved remarkably. The approach presented here would be attractive for surface modification of biomaterials.
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RSC Advances
·
2015
·Vol. 5
·期 83
·68200-68208
·
IF 3.84
Yuezhong Wen,Ying Liang,Chensi Shen,Hongkai Wang,Dongmei Fu,Hongyu Wang
A green efficient strategy for the combination of biodegradation and adsorption methods is urgently desired nowadays to degrade dyes in an economical and effective way. In this study, a kind of novel, environmentally friendly and efficient hybrid for the fungus and chitosan–Fe membrane has been fabricated by the alginate approach. Myrothecium verrucaria I-5 and C-1, deuteromycete fungi capable of producing laccase to degrade dyes, have been used. It is found that the chitosan–Fe membrane can excellently immobilize the fungi mycelia and enzyme, and after the immobilization, the fungi and enzyme still maintain a high activity after the dye decolorization. Also, the immobilized fungi membranes have been proved to have a high efficiency for dye decolorization. Simultaneously, the decolorization ability of the crude enzymes, which are extracted from I-5 and C-1 medium, and the decolorization ability of chitosan–Fe membrane are also investigated, respectively. The results demonstrate that the dyes can be removed by the fungi and the membrane synergistically. In addition, different kinds of dyes are successfully removed with chitosan–Fe immobilized fungi membranes. These results demonstrate that dyes are first adsorbed on the chitosan–Fe membrane, decreasing the toxicity to the fungus, and then bio-degraded by the laccase produced from the fungi on the membrane.
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FOOD CONTROL
·
2015
·
IF 2.806
Zhiyong Zhao,Na Liu,Lingchen Yang,Jianhua Wang,Suquan Song,Dongxia Nie,Xianli Yang,Jiafa Hou,Aibo Wu
The primary objective of this study was to synthesize three types of cross-linked chitosan polymers and further investigate their adsorption capability for multiple mycotoxins , including aflatoxin B 1 (AFB 1 ), ochratoxin A (OTA), zearalenone (ZEN), fumonisin B 1 (FB 1 ), deoxynivalenol (DON) and T-2 toxin (T2). Among these synthetic adsorbents, cross-linked chitosan-glutaraldehyde complex presented the highest adsorption capability for AFB 1 (73%), OTA (97%), ZEN (94%) and FB 1 (99%), but no obvious adsorption for DON and T2 (<30%). The effect of various incubation conditions (contact time, dosage and pH) was also studied. Subsequently, the experimental data were fitted to Langmuir, Freundlich and Hill models. The best fitting model to describe AFB 1 and FB 1 adsorption was Langmuir model ( R 2 ≥ 0.99), with the theoretical maximum adsorption amounts of 5.67 mg/g for AFB 1 and 15.7 mg/g for FB 1 . The Hill model was the best model for OTA and ZEN adsorption ( R 2 > 0.98), with the predicted maximum adsorption amounts were 24.8 mg/g for OTA and 9.18 mg/g for ZEN. In addition, the adsorption capability of adsorbent for the simultaneous presence of multiple mycotoxins was also evaluated in buffer system and simulated gastrointestinal condition. The results indicated that the coexisted multiple mycotoxins didn't affected the adsorption capability of adsorbent, whereas the adsorption amounts of toxins were decreased by some gastrointestinal components. The findings of this research suggest that chitosan–glutaraldehyde complex has the potential to be applied as multitoxin adsorbent material for reducing the combined adverse effect of multiple mycotoxins on humans and animals.
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