CARBOHYDRATE RESEARCH
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2026
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IF 3.4
Chenyu Wang,Guannan Yu,Qian Liu,Lichun Ye,Shilei Wang,Guohua Zheng,Junjie Hu,Lei Xu,Yan Meng,Zhaohua Shi
Recently, low-temperature water isolation has been confirmed to better keep the original structure of polysaccharides in plant. Thus, in this work, we obtained a new polysaccharide (WPVP) by water extraction at room-temperature (RT) from Prunella vulgaris L., a famous traditional edible Chinese medicinal herb. Unlike most reported complex heteropolysaccharides from Prunella vulgaris L. by hot water, the polysaccharide we obtained at RT was identified as an arabinoxylan, which is comprised a β -1,4-D-xylopyranose (Xyl p ) backbone substituted at O-2 with linear α -1,5-L-arabinofuranose (Araƒ) side chains. Notably, this new WPVP significantly ameliorated alcohol-induced hepatic damage in mice through gut regulation, as indicated by the improvement on hepatic lipid accumulation, inflammation, and intestinal microecology. Collectively, this work suggests a new strategy for polysaccharides isolation to maintain the original structure, and also proposes a novel therapeutic strategy for alcoholic liver disease (ALD).
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Current Plant Biology
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2026
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IF 5.1
Qiyue Zhang,Liqi Miao,Yongmiao Wang,Yupeng Du,Zihao Shen,Yuzhuo Dong,Zhichao Xu,Ya Tian
Liriodendron tulipifera is a rich source of structurally diverse aporphine benzylisoquinoline alkaloids (BIAs), which exhibit various pharmacological activities including antioxidant, antitumor, and anti-rheumatic effects, indicating significant medicinal potential. Nevertheless, the clinical development of these compounds is limited by their low natural abundance in planta and poor synthetic biology yields, with enzyme resources and catalytic efficiency being the primary limiting factors for the latter. Methyltransferases (MTs) are critical for determining aporphine alkaloid diversity and bioactivity but remain largely uncharacterized in L. tulipifera . Here, we integrated genomic and evolutionary analyses to reveal a whole-genome duplication event in L. tulipifera , with expanded gene families significantly enriched in secondary metabolic pathways. Phylogenetic analysis of key BIA pathway genes revealed that they share a common ancestry between Magnoliids and Ranunculales, but evolved independently in Sapindales. Based on genomic data, a total of 65 methyltransferase genes (including 58 OMTs and 7 NMTs) were identified. Through phylogenetic analysis and expression pattern profiling, eight OMT and two NMT genes were selected as candidates. Enzyme activity assays demonstrated that LtOMT37 and LtOMT44 catalyze C7 O -methylation, while LtNMT4 and LtNMT6 catalyze N -methylation of multiple BIA pathway substrates. This study reports the first characterization of 7- O -methyltransferases in the Liriodendron , laying a foundation for deciphering the aporphine BIA pathway in magnoliids and providing new genetic resources for the sustainable production of BIAs.
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Journal of Translational Medicine
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2026
·Vol. 24
·期 1
·335
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IF 9.7
Li Tong,Li Yifei,Gao Jiaxiang,Ma Ruichen,Peng Yue,Zhang Qidong,Chen Yingzhi,Wang Weiguo
Background Steroid-induced osteonecrosis of the femoral head (SONFH) lacks effective early-stage interventions, and its systemic drivers remain incompletely defined. Luteolin (Lut) is reported to be protective, but the in vivo mechanism is unclear. We investigated whether Lut acts through a gut microbiota–metabolite–innate immunity axis in SONFH. Methods Male Sprague–Dawley rats underwent SONFH induction using lipopolysaccharide plus methylprednisolone and received oral Lut or L-Carnitine; gut microbiota dependence was examined using a four-antibiotic depletion regimen. Femoral heads were evaluated by histology and micro-computed tomography. Mechanisms were interrogated by integrated 16S rRNA profiling, untargeted serum Liquid Chromatography–Mass Spectrometry metabolomics, and femoral-head transcriptomics. Dexamethasone (Dex)-injuried ROS17/2.8 osteoblasts and HMEC-1 endothelial cells were used for functional validation, and IFIH1 was silenced by shRNA to test pathway necessity. Results Lut preserved trabecular microarchitecture and reduced empty osteocyte lacunae in vivo, yet minimally rescued Dex-injured osteoblasts and endothelial cells, indicating an indirect mechanism mediated by gut microbiota-derived metabolic changes. Microbiota depletion abolished Lut’s in vivo benefit, establishing microbiota dependence. Metabolomics identified an increase in circulating L-Carnitine that correlated with four bacterial genera. Exogenous L-Carnitine restored cell proliferation, reduced apoptosis, and improved endothelial tube formation, and in vivo improved trabecular indices while lowering inflammatory cytokines (IL-1β, IL-6, TNF-α). Integrated multi-omics highlighted an interferon-stimulated gene module (OAS1A, HERC6, IFIH1, IFI44), with IFIH1 most strongly associated with disease severity. L-Carnitine attenuated Dex-induced IFIH1 expression, and IFIH1 knockdown mimicked L-Carnitine and eliminated its additional anti-inflammatory effect. Conclusions Lut alleviates SONFH via gut microbiota-driven elevation of L-Carnitine, which constrains IFIH1 expression and inflammation to preserve femoral-head trabecular microarchitecture. Targeting the microbiota–L-Carnitine–IFIH1 axis may enable mechanism-based early intervention strategies for SONFH.
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BMEMat
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2026
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IF 17.2
Siyu Pan,Xuanyi Lu,Wenjie Yang,Yunhui Peng,Lingfu Deng,Shuai Zha,Xueyang Fang,Guochen Bao,Lijun Jiang
Intratumoral L-lactate represents a valuable biomarker for both prognosis and therapy response, underscoring the importance of its accurate detection. However, traditional L-lactate assays rely on indirect hydrogen peroxide readouts and often compromise the accuracy and specificity. Herein, we report an aptamer-based nanoprobe (UCNP-Apt-Act) that allows direct L-lactate detection. Aptamers offer high specificity for L-lactate but suffer from poor cellular uptake and premature activation by extracellular L-lactate. To overcome this, we introduced lanthanide-doped upconversion nanoparticles (UCNPs) to enable spatially controlled unlocking by near-infrared (NIR) light for intratumoral L-lactate detection. The nanoprobe remains “locked” during delivery via an engineered actuator, preventing premature response to extracellular L-lactate. Upon 980 nm excitation, UCNPs convert NIR to UV emission, photocleaving the actuator linker and unlocking the aptamer. Notably, the UCNP carriers enhanced cellular uptake by 41 times through overcoming membrane barriers. The unlocked nanoprobe shows a 46-fold fluorescence enhancement in the presence of L-lactate, with an exceptional selectivity against a broad range of molecules and biological ions and metabolites. It enables specific detection of L-lactate in cancer cells, colorectal tumors, and diverse real samples, with excellent recoveries of 95.0%–110.0%. This strategy establishes a robust approach for endogenous small-molecule sensing and provides a versatile platform for biomedical applications.
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POLYMER
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2025
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IF 4.5
Lina Meng,Yanan Sun,Zhuoming Duan,Yujing Zhang,Dongmei Liu,Zhe Wang,Yibo Liu,Mingyue Sun,Chuanhui Gao,Yuetao Liu
Portulaca oleracea L. exhibits a wide range of pharmacological effects, including analgesic, anti-inflammatory, anti-oxidant, anti-bacterial, and wound healing properties. Flexible hydrogels have shown potential for clinical applications due to their excellent moisturizing properties and biocompatibility as wound dressings. However, the use of Portulaca oleracea L. extract in hydrogel wound dressings has been less reported. In this study, a novel self-adhesive hydrogel was prepared and loaded with Portulaca oleracea L. extract. The hydrogels demonstrated outstanding mechanical strength and exhibited good tissue adhesion, biocompatibility and anti-bacterial activity. Furthermore, the abundant natural alkaloids and flavonoids in Portulaca oleracea L. provided additional anti-inflammatory and anti-bacterial effects on wounds. According to the study involving mice with skin incisions, the hydrogel exhibited a high wound closure effect and offered a promising strategy for rapid skin wound healing. The combination of Portulaca oleracea L. as a natural biological substance with hydrogel materials gave inspirational implications in wound dressing development.
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INDUSTRIAL CROPS AND PRODUCTS
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2026
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IF 6.2
Qiqi Feng,Qing Wang,Tao Wang,Hongyu Pan,Yujie Zhong,Yongliang Zhuang
Glycation promotes the generation of advanced glycation end products (AGEs), which in turn exacerbate inflammation, oxidative stress, and chronic metabolic disorders. Given the potential side effects associated with synthetic antiglycation agents, a natural polyphenol-based antiglycation agent was explored from the Tamarindus indica L. shell. In this study, Tamarindus indica L. shell polyphenols (TSP) were obtained via ultrasound-assisted extraction combined with AB-8 macroporous resin purification, which yielded the highest levels of phenolics and flavonoids. UPLC-Q-Orbitrap-MS² analysis identified 23 phenolic compounds, with flavonoids constituting the predominant class, including (+)-catechin, luteolin, and apigenin. TSP exhibited strong antiglycation activity, with dose-dependent inhibition of Amadori intermediates and AGEs. Mechanistic investigations revealed that TSP effectively trapped reactive carbonyl species (RCS), reduced protein oxidation and cross-linking, and maintained protein secondary structure. Spectroscopic analyses and molecular docking further confirmed stable noncovalent interactions between TSP constituents and key glycation-prone residues (arginine and lysine) in bovine serum albumin. Among the identified monomers, (+)-catechin, luteolin, apigenin, diosmetin, 4-hydroxybenzoic acid, procyanidin A2, and eriodictyol-7-O-glucoside exhibited the greatest antiglycation effects. Overall, this study demonstrated that TSP effectively inhibited glycation by trapping RCS, suppressing protein oxidation and structural alteration, and establishing specific interactions with key amino acid residues in proteins. The innovation of this study lies in systematically developing TSP as a novel natural antiglycation agent, demonstrating its multi-mechanistic efficacy and identifying key active constituents, thereby offering a sustainable strategy to valorize agricultural by-products.
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ACS Applied Materials & Interfaces
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2019
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IF 8.456
Minghu Zhao,Zhen Huang,Shixing Wang,Libo Zhang,Yang Zhou
Mercury ions can cause a series of hazards to humans and the environment, even in trace amounts. Here, we designed a novel adsorbent (Cys-UiO-66) by functionalizing NH2-UiO-66 with l-cysteine for selective removal of Hg(II) from solution. The Cys-UiO-66 was characterized by different instruments. The adsorption property of Cys-UiO-66 was evaluated by batch methods. The maximum adsorption capacity was 350.14 mg/g at pH 5.0. Furthermore, the adsorption isotherm and kinetics models were in accord with the Langmuir and pseudo-second-order models, respectively, evidencing that the adsorption behavior was dominated by monolayer chemisorption. The Cys-UiO-66 had better affinity for Hg(II) than other coexisting ions in wastewater and could be regenerated for at least five cycles. The results prove that Cys-UiO-66 is a talented and efficient sorbent for mercury ions.
研究领域:
环境化学
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材料科学
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吸附技术
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重金属去除
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水处理
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2016
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IF 3.782
Hui Zhang,Jiangkang Xu,Liang Xing,Jianbo Ji,Aihua Yu,Guangxi Zhai
Doxorubicin (DOX) is one of the most common chemotherapeutic agents for the treatment of various cancers, but its clinical usage is limited by dose-dependent cardiotoxicity. We have recently synthesized a series of Chondroitin sulfate/poly ( d , l -lactideco-glycolide) block copolymers (Chs- b -PLGA) with different length of hydrophobic block by an end-to-end coupling strategy. The structure of the amphiphilic block copolymers with low critical micelle concentration (∼28 mg/L) were confirmed by 1 H NMR. The copolymers could self-assemble into stable micelles in aqueous environment with homogeneous size distribution and negative zeta potential . The hemolytic study indicated their excellent blood compatibility and potential application for intravenous administration. DOX can be efficiently encapsulated by the Chs- b -PLGA micelles. The DOX-loaded micelles showed a sustained and pH dependent drug release behavior. The cytotoxicity assay showed no associated toxicity with blank micelles while concentration-related cell inhibition with DOX-loaded micelles. Moreover, fast cellular uptake of DOX-loaded micelles was observed by fluorescent microscope. In vivo pharmacokinetics study showed that the Chs- b -PLGA micelles could significantly prolong the blood circulation time of DOX. The maximum tolerated dose (MTD) of DOX-loaded micelles in Kunming mice was about 2-fold higher of the MTD of free DOX, indicating a high tolerance of the DOX-loaded micelles. In conclusion, the Chs- b -PLGA micelles would be a potentially useful drug delivery system for cancer therapy.
研究领域:
药剂学
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药物递送
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纳米医学
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高分子科学
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癌症治疗
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生物医学工程
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MOLECULES
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2026
·Vol. 31
·期 2
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IF 4.6
Xiaoyan He,Lisheng Tang,Ran Huang
With the growing demand for eco-friendly food packaging, poly(butylene adipate-co-terephthalate) (PBAT)/polylactic acid (PLA) composite films have emerged as promising biodegradable alternatives, but their inherent limitations (e.g., poor antioxidant capacity, weak UV stability, and insufficient antimicrobial activity) hinder practical applications. This study aimed to address these drawbacks by incorporatingRosmarinus officinalisL. extract (RM) as a natural multifunctional additive. PBAT/PLA/RM blend films with RM concentrations of 0.1%, 0.3%, 0.5%, and 1% were fabricated via melt extrusion and blown film processing. Key characterizations were conducted to evaluate thermal stability, mechanical properties, morphology, antioxidant activity, UV-shielding performance, antimicrobial efficacy, and biodegradability. The results showed that RM significantly enhanced the antioxidant capacity of the films, with the highest DPPH radical scavenging activity achieved at 0.3% RM. UV-blocking performance improved incrementally with increasing RM concentration, and films containing ≥0.5% RM filtered over 90% of UVA and UVB radiation. All composite films met biodegradability standards, with over 90% degradation observed after 240 days of composting, though RM prolonged the initial degradation stage by inhibiting early microbial activity. However, the antimicrobial effect of RM was limited, and concentrations exceeding 1% caused film stickiness, impeding processing. This work demonstrates that RM is a viable natural additive for functionalizing PBAT/PLA films, offering enhanced antioxidant and UV-shielding properties while maintaining biodegradability, thus providing a promising solution for sustainable food packaging.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
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IF 6.2
Yuqi Gao,Min Lu,Jitong Wei,Dengyu Zheng,Hongliang Duan,Mingxu Wang,Zhao Ke,Zhongyi Wu,Qing X. Li,Zhongbao Zhang
Flavonoids, secondary phytometabolites, play many roles in human health and plant resistance, growth, and development. They are commonly glycosylated in the last step of their biosynthesis. In this study, a gene encoding an efficient flavonoid glycosyltransferase, ZmUGT76B1, was cloned from maize and expressed. The enzyme activity and function were characterized. ZmUGT76B1 catalyzed selective glycosylation at 3-, 7-, 3′-, and 4′-hydroxyl sites of flavonoids to form mono-O-glycoside and di-O-glycoside products. Moreover, ZmUGT76B1 had a broad substrate preference and strict sugar donor selectivity. Guided by simulated molecular docking and simulated mutagenesis, three ZmUGT76B1 mutants, Q338P, E361P, and Q338P/E361P, were constructed. The mutation of either of the two sites substantially reduced the enzyme activity, whereas the double mutation eliminated the activity. The determination of enzyme activity and prediction of the active site of this enzyme have expanded our knowledge of maize uridine diphosphate-dependent glycosyltransferases, providing an important glycosyltransferase for metabolic engineering of mono- and dioxygenated glucosyl flavonoids.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9
Ruohan Liang,Dongsheng Zhao,Fangming Jin,Jun Xu,Guicai Liu
The limited mono−/divalent anion selectivity and inadequate antifouling properties of polyamide (PA) nanofiltration (NF) membranes restrict their use in water treatment. Herein, L-histidine (L-His)-modified NF membranes were prepared through interfacial polymerization on a polyethersulfone porous substrate. The aqueous-phase comonomers were piperazine and L-His, which contain both amino and carboxyl groups, while the organic-phase monomer was trimesoyl chloride. Compared with the NF0 membrane without addition of L-His, all of the L-His-modified membrane was characterized by a higher surface roughness, a thinner PA layer, greater hydrophilicity, a more negative surface charge and a larger mean pore size. Among them, the optimal NF0.5 membrane with the addition of 0.5% ( w / v ) L-His demonstrated a pure water permeability of 17.9 L/(m 2 ·h·bar) and a 2.7-fold higher NaCl/Na 2 SO 4 selectivity (up to 102.0). In particular, the NaCl/Na 2 SO 4 selectivity improved with higher operating pressure, but decreased with increasing single-salt concentration or NaCl/Na 2 SO 4 mass ratio in the feed solution. In addition, the NF0.5 membrane demonstrated superior resistance to protein fouling and outstanding long-term operational stability throughout a continuous 7-day filtration process. This study could provide valuable insights into the development of NF membranes with high mono−/divalent anion selectivity and excellent antifouling properties.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.1
Xiao-Han Yuan,Wen-Sheng Liu,Jian-Hua Zhang
Formaldehyde (FA), the simplest and widely-applied aldehyde, can pose serious health issues when present at elevated concentrations. Herein, this study proposes a self-calibrating ratiometric sensing strategy for the sensitive detection of FA, which is based on the dual-functional ligands and multi-metal synergistic effects of L-Histidine (L-His)-functionalized Lanthanide metal-organic gels (MOGs). In this strategy, L-His and 1,3,5-benzenetricarboxylic acid (BTC) served as functional ligands coordinating with Zr 4+ , Eu 3+ , and Tb 3+ metal centers, forming three MOGs with superior photoluminescence properties: ZrEuTb-His-MOG, ZrEu-His-MOG, and ZrTb-His-MOG. Notably, ZrEuTb-His-MOG exhibits a prominent multi-metal synergistic effect: a stable self-calibration signal is generated through Zr 4+ -(L-His) ligand-to-metal charge transfer (LMCT), while the antenna effect of BTC to Tb 3+ facilitates a FA-responsive specific fluorescent signal. The synergistic interaction between these two mechanisms establishes a highly reliable self-calibrating detection system. This sensor achieves high sensitivity with a detection limit of 8.4 nM, a broad linear range of 0.001 μM–60 μM, and excellent selectivity against common interfering species. Moreover, ZrEuTb-His-MOG exhibits outstanding regenerability and long-term signal stability, qualifying it as an efficient and recyclable fluorescent probe for FA detection. Additionally, we developed a portable fluorescent test strip based on ZrEuTb-His-MOG, enabling “on-off” visual detection of both aqueous and gaseous FA with a fast response. This work provides a novel and reliable strategy for on-site FA monitoring in environmental monitoring, food safety, and public health protection.
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