Chinese Journal of Integrative Medicine
·
2021
·Vol. 28
·期 10
·909-917
·
IF 1.978
Yue Wu-heng,Qin Lan-qun,Cai Juan,Mei Rui,Qian Han-qing,Zou Zheng-yun
Objective To verrify the anti-tumor efficacy and toxicity between juglone (Jug) and Jug-loaded PLGA nanoparticles (Jug-PLGA-NPs). Methods Jug-PLGA-NPs were prepared by ultrasonic emulsification. The anti-tumor activity of Jug (2, 3, 4 µg/mL) and Jug-PLGA-NPs (Jug: 2, 3, 4 µg/mL) in vitro was measured by MTT assay and cell apoptosis analysis. The distribution, anti-tumor effect and biological safety in vivo was evaluated on A375 nude mice. Results With the advantage of good penetration and targeting properties, Jug-PLGA-NPs significantly inhibited proliferation and migration of melanoma cells both in vitro and in vivo ( P <0.05 or P <0.01) with acceptable biocompatibility. Conclusions Jug can inhibit the growth of melanoma but is highly toxic. With the advantage of sustained release, tumor targeting, anti-tumor activity and acceptable biological safety, Jug-PLGA-NPs provide a new pharmaceutical form for future application of Jug.
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ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 7
·1749-1757
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IF 6.15
Li Dan,Ding Jin,Liu Te-li,Wang Feng,Meng Xiang-xi,Liu Song,Yang Zhi,Zhu Hua
The spike protein of SARS-CoV-2 interacts with angiotensin-converting enzyme 2 (ACE2) of human respiratory epithelial cells, which leads to infection. Furthermore, low-dose radiation has been found to reduce inflammation and aid the curing of COVID-19. The receptor binding domain (RBD), a recombinant spike protein with a His tag at the C-terminus, binds to ACE2 in human body. We thus constructed a radioiodinated RBD as a molecule-targeted probe to non-invasively explore ACE2 expression in vivo, and to investigate radiotherapy pathway for inhibiting ACE2. RBD was labeled with [ 124 I]NaI using an N-bromosuccinimide (NBS)-mediated method, and 124 I-RBD was obtained after purification with a specific activity of 28.9 GBq/nmol. Its radiochemical purity was (RCP) over 90% in saline for 5 days. The dissociation constant of 124 I-RBD binding to hACE2 was 75.7 nM. The uptake of 124 I-RBD by HeLa ACE+ cells at 2 h was 2.96% ± 0.35%, which could be substantially blocked by an excessive amount of RBD, and drop to 1.71% ± 0.23%. In BALB/c mice, the biodistribution of 124 I-RBD after intravenous injection showed a moderate metabolism rate, and its 24 h-post injection (p.i.) organ distribution was similar to the expression profile in body. Micro-PET imaging of mice after intrapulmonary injection showed high uptake of lung at 1, 4, 24 h p.i.. In conclusion, the experimental results demonstrate the potential of 124 I-RBD as a novel targeted molecular probe for COVID-19. This probe may be used for non-invasive ACE2 mapping in mammals.
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JOURNAL OF NANOPARTICLE RESEARCH
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2021
·Vol. 23
·期 10
·1-15
·
IF 2.253
Guo Zhengqiang,Liu Zheng,Liu Wangyu,Tang Aimin,Chen Wenjin,Luo Shijie
Recently, hydrogels have attracted considerable interests due to their intrinsic flexibility and adjustable mechanical properties. However, the integration of high conductivity, enhanced mechanical performance, and plasticity into one single hydrogel is still challenging. In this work, TEMPO-oxidized cellulose nanofibers (TOCNF, diameter 20–50 nm, length \(\ge\) 1 um) are used as the efficient dispersant of multiwalled carbon nanotubes (MWCNTs, out diameter 5–15 nm, length 10–30 um). Conductive hydrogels are prepared through a simple one pot process and freeze–thaw cyclic method in combination with Fe 3+ ions and TOCNF-MWCNT dispersions in polyvinyl alcohol (PVA) hydrogels. The resulting hydrogels demonstrate high water content (~ 88.8%), mechanical properties (ultimate stress of 1.1 MPa, strain of 336%), and conductivity (0.57 S/m), which can be applied to the preparation of stress or strain sensors. The hydrogel-based resistance sensor is applied to human motion detection, showing a sensitivity of 1.876 kPa −1 with pressure lower than 16 kPa. Similarly, the hydrogel-based capacitive sensor can detect imperceptible pressure of 20 Pa. In addition, the hydrogels also reveal excellent plasticity. In conclusion, the conductive hydrogel possesses excellent comprehensive properties, providing inspiration and potential for the preparation and application of wearable strain sensors.
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POLYMER JOURNAL
·
2021
·Vol. 54
·期 1
·47-55
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IF 3.08
Jiao Danhua,Cai Xiaodong,Song Qun,Zhou Ruiqun,Peng Xiao,Bao Dongmei
A series of aliphatic poly(carbonate-co-ester)s (PBCFs) were successfully synthesized via melt polycondensation of 1,4-butanediol (BD) and dimethyl carbonate (DMC) in the presence of biobased fumaric acid (FA). The prepared unsaturated copolymers had weight-average molecular weights ( M w ) in the 4.98 × 10 4 to 8.82 × 10 4 g·mol −1 range and from 1.56 to 1.83 polydispersities. They all had random microstructures. The physical properties of the novel poly(carbonate-co-ester)s, such as melting temperature ( T m ) and melting enthalpy ( ∆H m ), varied widely depending on their composition. The stiff fumaric acid units in the poly(butylene carbonate) (PBC) chain caused an increase in the glass transition temperature ( T g ) and thermal stability. Furthermore, PBCF10 to PBCF20 (with fumaric acid unit content of <19%) contained PBC crystals, while the lattice structure of PBCF40 to PBCF80 (with fumaric acid unit content greater than 37%) was similar to that of the poly(butylene fumarate) (PBF) type. The mechanical properties and biodegradability of the copolymers were also studied.
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
·
2021
·Vol. 101
·期 1
·215-226
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IF 2.326
Li Ping,Zhao Liuyong,Wang Song,Zhang Xiaohui,Jiang Huaiyuan,Liu Ruizhen,Zhao Nengchuang,Cheng Jian,Xu Caili,Zhao He,Lyu Renliang
Known as one efficient upconversion luminescent material, Rare-earth ions doped natrium yttrium fluoride NaYF 4 :Yb/Re has broad application prospects. However, due to the limitation of the existing preparation methods, it is difficult to realize industrial production. In this paper, the upconversion luminescence microparticles, rare earth ions doped Natrium Yttrium Fluoride NaYF 4 :Yb/Er, were synthesized productively by impinging stream method. Synthesis conditions were optimized as EDTA: Ln to 0.5 and NaF: Ln to 6 (Ln represents the sum of moles of Y, Yb, and Er), reacting temperature and time to 50 °C and 3 h to obtain a high yield of 99.92% and particle with good monodispersity. To get strong upconversion luminescence, the effect of the calcination temperature and the amount of EDTA were investigated. The bright upconversion emissions by excited with laser radiation at 975 nm were found at 510–690 nm. The higher the calcination temperature, the stronger the luminescence and the strongest luminescence occurs when calcined at 600 °C. And the adding of EDTA will decrease the luminescence intensity. However, the sintering of particles at high temperatures and aggregation of particles without EDTA are unfavorable for the application of the products. Based on the above considerations, the optimal calcination temperature was determined at 450 °C, and the ratio of EDTA: Ln is preferred at 0.5. The impinging stream method to prepare upconversion material has the advantages of being simple in the process and equipment, high in output, low in investment, and convenient to be applied and popularized.
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CURRENT MICROBIOLOGY
·
2021
·Vol. 78
·期 12
·4084-4097
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IF 2.188
Ahmad Manzoor,Ling Juan,Yang Qingsong,Sajjad Wasim,Zhou Weiguo,Yin Jianping,Dong Junde
Seagrass meadows constitute a prestigious ecosystem in the marine environment, providing valuable ecological and commercial services. Among the various causes, pollutions are considered one of the significant reasons for seagrass decline globally. This study investigates the impacts of polycyclic aromatic hydrocarbons mixture (pyrene, phenanthrene, and fluorene) on bacterial communities in Halophila ovalis sediments. The seagrass sediment bacterial microbiome was evaluated in a batch culture experiment by Illumina MiSeq sequencing. Culture-able bacterial strains were isolated and characterized by 16S rRNA gene sequencing. The results demonstrated an excellent alpha diversity in the original sediments with a Shannon index of (8.078) compared to the subsequent control group (5.908) and PAH-treated group (PAH-T) (4.916). Three phyla, Proteobacteria , Firmicutes, and Bacteroidetes, were detected in high abundance in the control and PAH-T groups. However, a significant difference ( P < 0.05) was observed at the genus level between control and PAH-T group bacterial consortia. Pseudomonas, Mycobacterium, Idiomarina, Hydrogenophaga, Alteromonas, Sphingobacterium, and several others were highly abundant in PAH-T groups. Most of the culture-able isolates recovered in this study showed the closest resemblance to previously identified hydrocarbon-degrading bacteria. Among the three strains, Mix-16 ( Citricoccus yambaruensis ) and Mix-20 ( Gordonia rubripertincta ) showed a higher degradation of PAHs than Mix-19 ( Isoptericola halotolerans ) in the monoculture experiment. The most increased degradation of PAHs was recorded in the co-culture experiment. The present work revealed that PAHs could act as environmental stress and can influence bacterial community succession. Moreover, the co-culture strategy significantly enhanced the biodegradation of PAHs.
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CELLULOSE
·
2021
·Vol. 28
·期 18
·11429-11450
·
IF 5.044
Zhao Yali,Lu Wenxi,Shen Shangzhu,Wei Li
The hydrogel wound dressings are mostly sheet-like and do not possess adhesive properties, so it is often difficult for them to adhere well to the skin tissues and fit completely with the irregularly shaped wounds or deep wounds, thereby affecting their therapeutic effects. Herein, the chitosan derivative-based mussel-inspired hydrogels have been developed to overcome these obstacles. Specifically, the principle of adhesion of marine mussel was imitated to synthesize catechol-modified chitosan (CMC), which was potentially used as the raw material to prepare the mussel-inspired hydrogels, that can contribute to the excellent adhesive property and make the hydrogels adhere well to the wound sites. The o-diphenyl groups in CMC could be easily oxidized to form o-diquinone to further form a covalent binding with amino groups (–NH2), or can directly form coordination binding with Fe 3+ .This strong covalent and coordination binding causes the hydrogels to possess a complex spatial structure, which facilitates the hydrogels to display good swelling properties, drug loading and release properties. Furthermore, the total saponins of Panax notoginseng were loaded in the hydrogels and the therapeutic effects of drug-loaded hydrogel were investigated in the model of full-thickness skin injury in mice. The wound healing rate was observed to be as high as 92.5% without exhibiting any scarring. Overall, our findings provided a novel strategy for the preparation of hydrogels for therapeutic applications.
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 29
·期 12
·17866-17877
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IF 4.223
Gao Yu-Qiong,Zhou Jin-Qiang,Ning Han,Rao Yan-Yan,Gao Nai-Yun
In this study, electrochemically activated peroxymonosulfate (EC/PMS) with a sacrificial iron electrode was used for the removal of chloramphenicol (CAP) from water. Compared to electrolysis alone, peroxymonosulfate (PMS) alone, and Fe 2+ /PMS, EC/PMS significantly enhanced the CAP degradation. Various parameters, such as the applied current, electrolyte concentration, and PMS dose, were investigated to optimize the process. In addition, acidic conditions facilitated the CAP degradation. The presence of Cl − slightly enhanced the CAP degradation, while both HCO 3 − and NO 3 − exhibited an inhibitory effect on the CAP degradation. The floccules were also analyzed after the reaction by XPS and XRD. Quenching experiments indicated that both sulfate radicals (SO 4 ●− ) and hydroxyl radicals (•OH) were responsible for the CAP degradation. In addition, the degradation products were identified by LC/TOF/MS, and the degradation pathways were proposed accordingly. These results indicated that EC/PMS is a promising treatment process for the remediation of water polluted by CAP.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-14
·
IF 10.435
Wei Zhuxin,Duan Guangxin,Huang Baoxing,Qiu Shanshan,Zhou Dandan,Zeng Jianfeng,Cui Jiabin,Hu Chunhong,Wang Ximing,Wen Ling,Gao Mingyuan
Background Fluorescence imaging as the beacon for optical navigation has wildly developed in preclinical studies due to its prominent advantages, including noninvasiveness and superior temporal resolution. However, the traditional optical methods based on ultraviolet (UV, 200–400 nm) and visible light (Vis, 400–650 nm) limited by their low penetration, signal-to-noise ratio, and high background auto-fluorescence interference. Therefore, the development of near-infrared-II (NIR-II 1000–1700 nm) nanoprobe attracted significant attentions toward in vivo imaging. Regrettably, most of the NIR-II fluorescence probes, especially for inorganic NPs, were hardly excreted from the reticuloendothelial system (RES), yielding the anonymous long-term circulatory safety issue.Results Here, we develop a facile strategy for the fabrication of Nd3+-doped rare-earth core–shell nanoparticles (Nd-RENPs), NaGdF4:5%Nd@NaLuF4, with strong emission in the NIR-II window. What’s more, the Nd-RENPs could be quickly eliminated from the hepatobiliary pathway, reducing the potential risk with the long-term retention in the RES. Further, the Nd-RENPs are successfully utilized for NIR-II in vivo imaging and magnetic resonance imaging (MRI) contrast agents, enabling the precise detection of breast cancer.Conclusion sThe rationally designed Nd-RENPs nanoprobes manifest rapid-clearance property revealing the potential application toward the noninvasive preoperative imaging of tumor lesions and real-time intra-operative supervision.Graphical abstract
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Scientific Reports
·
2021
·Vol. 11
·期 1
·1-10
·
IF 4.38
Zhang Xiaofang,Wang Liuyang,Liu Chunqin,Liu Yongqiang,Mei Xiangdong,Wang Zhongyue,Zhang Tao
The white-spotted flower chafer (WSFC), Protaetia brevit arsis Lewis, is native to East Asia. Although their larvae are considered a potential resource insect for degrading plant residues, producing protein fodder, and processing to traditional medicine, adult WSFCs inflict damage to dozens of fruit and economic crops. The control of the WSFC still relies heavily on pesticides and the inefficient manual extraction of adults. Here, we report the identification and evaluation of the aggregation pheromone of WSFCs. From the headspace volatiles emitted from WSFC adults, anisole, 4-methylanisole, 2-heptanone and 2-nonanone were identified as WSFC-specific components. However, only anisole and 4-methylanisole elicited positive dose–response relationship in electroantennography tests, and only 4-methylanisole significantly attracted WSFCs of both sexes in olfactometer bioassays and field experiments. These results concluded that 4-methylanisole is the aggregation pheromone of WSFCs. Furthermore, we developed polyethylene vials as long-term dispensers of 4-methylanisole to attract and kill WSFCs. The polyethylene vial lures could effectively attracted WSFCs for more than four weeks. Pheromone-based lures can be developed as an environmentally friendly protocol for monitoring and controlling WSFC adults.
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Journal of Advanced Ceramics
·
2021
·Vol. 11
·期 1
·120-135
·
IF 6.707
Lyu Yang,Du Baihe,Chen Guiqing,Zhao Guangdong,Cheng Yuan,Zhou Shanbao,Lv Qingrong,Zhang Xinghong,Han Wenbo
To further improve the oxidation resistance of polymer derived ceramic (PDC) composites in harsh environments, C f /SiC/SiHfBOC composites were prepared by chemical vapor infiltration (CVI) and precursor impregnation pyrolysis (PIP) methods. The weight retention change, mechanical properties, and microstructure of C f /SiC/SiHfBOC before and after oxidation in air were studied in details. Microscopic analyses showed that only the interface between the ceramics and fibers was oxidized to some extent, and hafnium had been enriched on the composite surface after oxidizing at different temperature. The main oxidation products of C f /SiC/SiHfBOC composites were HfO 2 and HfSiO 4 after oxidation at 1500 °C for 60 min. Moreover, the weight retention ratio and compressive strength of the C f /SiC/SiHfBOC composites are 83.97% and 23.88±3.11 MPa, respectively. It indicates that the C f /SiC/SiHfBOC composites should be promising to be used for a short time in the oxidation environment at 1500 °C.
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Nanoscale Research Letters
·
2021
·Vol. 16
·期 1
·1-8
·
IF 4.703
Qu Zewen,Li Shi,Feng Wenshuai,Kan Shuting,Gao Xiaohui,Guo Aimin,Li Hongjian,Deng Lianwen,Huang Shengxiang,Zhao Yan,Chen Wei
An accurate sensor to rapidly determine the glucose concentration is of significant importance for the human body health, as diabetes has become a very high incidence around the world. In this work, copper nanoparticles accommodated in porous carbon substrates (Cu NP@PC), synthesized by calcinating the filter papers impregnated with copper ions at high temperature, were designed as the electrode active materials for electrochemical sensing of glucose. During the formation of porous carbon, the copper nanoparticles spontaneously accommodated into the formed voids and constituted the half-covered composites. For the electrochemical glucose oxidation, the prepared Cu NP@PC composites exhibit much superior catalytic activity with the current density of 0.31 mA/cm 2 at the potential of 0.55 V in the presence of 0.2 mM glucose. Based on the high electrochemical oxidation activity, the present Cu NP@PC composites also exhibit a superior glucose sensing performance. The sensitivity is determined to be 84.5 μA /(mmol . L) with a linear range of 0.01 ~ 1.1 mM and a low detection limit (LOD) of 2.1 μmol/L. Compared to that of non-porous carbon supported copper nanoparticles (Cu NP/C), this can be reasonable by the improved mass transfer and strengthened synergistic effect between copper nanoparticles and porous carbon substrates.
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