INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Lijun Zhu,Wenjie Huang,Jingjing Shi,Fei Li,Xiuxia Sun,Jianxi Xiao
The degradation of elastin in aged skin results in the loss of elasticity, the formation of wrinkles, and even a reduced ability for wound healing after injury. However, previous studies about elastin material have been limited by high immunogenicity of animal-derived elastin, the use of single elastin peptide sequences, and residual crosslinking agents. In this study, we designed a multipotent anti-aging recombinant human elastin (rElastin) and utilized it to counteract caudal fin shrinkage in zebrafish and D-galactose-induced intrinsic skin aging in mice. The rElastin demonstrated thermoresponsive reversible phase-transition closely resembling that of natural elastin. In vitro , cell experiments demonstrated that rElastin effectively promoted the proliferation, adhesion, and spreading of HFF-1 cells. In a D-galactose-induced intrinsic aging mouse model, rElastin significantly restored dermal density, skin hydration, and TEWL to levels approaching those of healthy skin. Histological analysis further confirmed that rElastin facilitated the deposition of collagen and regeneration of elastic fibers, thereby promoting structural and functional repair of aging skin. Additionally, rElastin obviously inhibited the tail fin shrinkage after UV radiation. rElastin effectively targets skin symptoms associated with intrinsic aging, providing fresh insights into anti-aging therapies and showcasing strong potential for applications in regenerative medicine and cosmetic dermatology.
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Food & Function
·
2025
·
IF 5.4
Le Wang,Houzhen Lu,Haitao Gui,Zifu Ni,Zhongke Sun,Zihua Wang,Zhihua Wang,Xuanyan Liu,Qipeng Yuan
Ulcerative colitis (UC) is a prevalent inflammatory bowel disease (IBD) posing a significant health threat. This study explored the protective effects of D-tagatose against DSS-induced colitis in mice and its underlying mechanisms using H&E staining, AB-PAS staining, immunofluorescence, immunohistochemistry, ELISA, qPCR, western blotting, and other assays. D-Tagatose improved colitis by increasing body weight and colon length, with decreased DAI (disease activity index) and histopathological scores. The results showed that D-tagatose inhibited the secretion of myeloperoxidase (MPO), inflammatory enzymes (iNOS and COX-2) and pro-inflammatory cytokines (TNF-α, IL-1β and IL-6) as well as increased the content of anti-inflammatory cytokines (IL-10) in vitro. In addition, D-tagatose enhanced the expression of tight junction proteins (ZO-1 and Occludin) and mucin (MUC-2). Furthermore, D-tagatose was able to modulate the gut microbiota dysbiosis caused by DSS-induced UC and increased the content of short-chain fatty acids (SCFAs). This study indicated that D-tagatose attenuated DSS-induced UC by modulating inflammatory cytokines, restoring intestinal barrier function, maintaining gut microbiota homeostasis, and enhancing SCFA production. These findings provide D-tagatose as a safe and effective novel functional food strategy for the prevention and treatment of UC.
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Biomaterials Science
·
2019
·Vol. 8
·期 3
·830-836
·
IF 5.251
Chengjun Wang,Ying Qian
A novel photosensitizer carbazolyl-BODIPY (Cz-BODIPY) with an orthogonal donor–acceptor structure was developed for photodynamic therapy (PDT). The photosensitizer Cz-BODIPY showed strong singlet oxygen sensitizing capability (ΦΔ = 0.68 in MeOH), excellent water solubility in dilute solution, and high photostability. The photosensitizer Cz-BODIPY exhibited negligible dark cytotoxicity and high phototoxicity (IC50 0.45 μM). Cz-BODIPY could induce cell apoptosis upon light illumination. Three cell states including living cells, apoptotic cells, and dead cells in the PDT process of Cz-BODIPY were determined via the Hoechst 33342/PI dual staining assays. The ROS (reactive oxygen species) generation in living cells during the PDT process of Cz-BODIPY was captured by the ROS detector, dihydroethidium (DHE). The photosensitizer Cz-BODIPY could be assimilated by zebrafish to generate ROS and diminish the integrity of zebrafish tissue upon light illumination. Tumor cell growth could be inhibited by Cz-BODIPY upon light illumination. The photosensitizer Cz-BODIPY displayed potential in real PDT application.
研究领域:
生物化学
·
药理学
·
肿瘤学
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ChemistrySelect
·
2025
·Vol. 10
·期 22
·e05941
·
IF 2
Yiyi Qiao,Jian Cao,Yanan Li,Chuanxiao Wang,Jiayu Gao,Linlin Wang,Xiao Zhang,Le Wang,Yuanhao Li,Zhicheng Zuo
Graphical “Donor-Acceptor” structural molecules with ICT and AIE properties were constructed. The sensor Nap-B1 was able to specifically recognize HSA by modifying with pyridinium and quinolinium. In the H2O-EtOH system or highly viscous and solid phase environment, the fluorescence of sensor was turned on in an aggregated. Elevated levels of human serum albumin (HSA) serve as an early indicator of cardiovascular and renal diseases in diabetic and hypertensive patients. This study focuses on the design and synthesis of two fluorescent probes, Nap-B1 and Nap-KL, which were developed by functionalizing the “D–A” structural framework consisting of naphthalimide derivatives (A) and triphenylamine (D) with pyridine/quinoline cations. These sensors are intended for the quantitative detection of HSA in urine, specifically recognizing HSA and inducing fluorescence “turn-on” upon binding. Both Nap-B1 and Nap-KL is demonstrated high selectivity for HSA, stability independent of environmental factors, and sensitivity for rapid detection. Changes in Zeta potential, dynamic light scattering (DLS), fluorescence lifetime, and fluorescence intensity were observed, which result from alterations in HSA conformation before and after sensor binding. Molecular docking experiments further confirmed that the probes bind to HSA via hydrophobic interactions and electrostatic adsorption, forming a probe-HSA complex and triggering the fluorescent response.
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Medicinal Chemistry
·
2017
·Vol. 13
·期 8
·753-760
·
IF 2.331
Guo Ping,Xie Zixin,Zhang Huan,Zhang Zaikui,Han Chao,Cheng Donghua,Lin Dan,Zhang Yuan,Wang Xuebao,Guo Xin,Ye Faqing
Background: Thienopyrimidinone is a newly designed, selective fibroblast growth factor receptor 1 (FGFR1) inhibitor with an excellent anticancer effect. Objective: The goal of the present study was to design and synthesize better FGFR1 inhibitors through modifications of the lead
compound thienopyrimidinone. Methods: In the present study, a series of C-2 substituted derivatives of thienopyrimidinone, namely L1-L16, were synthesized, and their inhibitory effects on FGFR1 were evaluated. The anti-proliferative activities of these compounds were assessed by MTT assay.
Results: Among the novel derivatives, L11 was found to exert remarkable FGFR1 inhibitory activity (79.93% at 10 μM) and anti-proliferative activity, with IC50 values of 2.1, 2.5, and 3.5 µM in the FGFR1-overexpressing cell lines, H460, HT-1197, and B16F10, respectively.
Conclusion: Our newly synthesized thienopyrimidinone derivatives may be candidate FGFR1 inhibitors for future development as novel anticancer agents.
研究领域:
药理学
·
肿瘤学
·
药物化学
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FUEL
·
2024
·
IF 6.7
Fengrui Li,Xiao Dai,Linfeng Zhang,Huadong Wu,Jianding Li,Jia Guo,Qun Yi
Conjugated microporous polymers (CMPs) with a donor–acceptor (D-A) molecular structure usually express the properties of well light-induced electron/hole separation efficacy and high photocatalytic activity. The triphenylamine (TPA) with a special three-dimensional propeller structure was chose as the electron donor, and the dibenzothiophene sulfone (BTDO) groups as the chain scaffold and electron acceptor to construct and prepare a unique “macrocyclic structure” D-A type CMPs photocatalyst (TPA-BTDO) for photocatalytic hydrogen production (PHP). In order to further improve the conjugation degree of the CMPs and enhance the electron transfer capacity and light absorption, the thiophene units was introduced as “π-bridge” to form a macrocyclic D-π-A type CMPs photocatalyst (TPA-T-BTDO). The results show that the addition of thiophene greatly improves the coplanarity between the donor–acceptor units, and the prepared TPA-T-BTDO-1 exhibited an amazing PHP efficiency of 65.41 mmol h −1 g −1 in the visible light region. More importantly, the PHP efficiency also reaches up to 39.63 mmol h −1 g −1 under the natural light conditions. Both the characterizations and DFT simulation results prove that the twisted structure of the TPA donor decreases the dihedral angle between the benzene rings of BTDO and TPA, which improves the coplanarity of polymer skeleton and then prevents the charges transfer along the polymer chain for recombination. This study highlights the significant influence of the geometry of the electron donor on the photocatalytic activity, as well as the strategy of the structure adjusting by adding suitable bridges between the donor–acceptor units.
研究领域:
材料科学
·
光催化
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RARE METALS
·
2026
·Vol. 45
·期 3
·e70185
·
IF 12.1
Chenyang Shu,Yimin Chen,Chuanlan Xu,Xinru Xu,Lingtao Sun,Jiayi Niu,Cherkasova Tatiana,Chaozhong Guo,Yuan Niu,Quan Xu
en This link goes to a English section zh This link goes to a Chinese section Platinum (Pt)-based catalysts are crucial for the commercialization of hydrogen/metal air batteries, but they suffer from Pt scarcity, high cost, and nanoparticle dissolution/agglomeration, mainly leading to performance degradation. We address this by synthesizing a PtFe alloy catalyst (PtFe–PA–NC) via a phytic acid (PA) assisted pyrolysis strategy, featuring Pt-skin-coated PtFe alloy nanoparticles on hierarchically porous N-doped carbon (NC). PA treatment simultaneously enhances the support's surface area and pore structure for better mass transport, and incorporates P into the carbon framework via P–C bonds. It can induce charge delocalization, regulating the electronic structure of PtFe alloys and strengthening Pt–Fe coupling. The Pt-skin suppresses Fe dissolution, whereas the PtFe core optimizes oxygen reduction reaction (ORR) intermediate adsorption (e.g., *OH). Theoretical calculations confirm that the Fe atom adjusts the d-band center of the Pt atom, intensifying oxygen binding and shifting the rate-determining step. Electrochemically, PtFe–PA–NC achieves a half-wave potential of 0.85 V and retains 95.8% of specific energy density after more than 140 h in zinc-air batteries. In alkaline fuel cells, it delivers a peak power density of 508 mW cm −2 at an ultralow Pt loading (0.017 mg cm −2 ), approximately twice that of 5 wt% Pt/C (0.025 mg cm −2 ). This work showcases synergistic electronic and interfacial regulation through heteroatom doping and alloying, promoting high-performance hydrogen/metal air batteries. 摘要 en This link goes to a English section zh This link goes to a Chinese section 铂基催化剂是氢/金属空气电池商业化的关键,但铂资源稀缺、成本高昂且纳米颗粒易溶解/团聚 , 是导致其性能衰减的主要原因。为此 , 本论文通过植酸(PA)辅助热解策略合成了一种 PtFe合金催化剂(PtFe–PA–NC) , 其结构为: Pt 壳层包覆的PtFe合金纳米颗粒负载于分级多孔氮掺杂碳(NC)载体上。通过植酸处理过程可同时实现了两个效果 : 一是提升了载体的比表面积与孔结构, 优化了传质效率 ; 二是通过 P–C 键将磷引入碳骨架,诱导电荷离域 , 从而调控 PtFe 合金的电子结构、强化 Pt–Fe 耦合作用。其中, Pt 壳层可抑制 Fe 的溶解, 而 PtFe 核则能优化氧还原反应(ORR)中间产物(如 * OH)的吸附行为。理论计算表明: Fe 原子可调节 Pt 原子的 d 带中心, 增强氧的结合作用并改变反应的决速步。电化学测试表明: 在锌空气电池中, PtFe–PA–NC 的半波电位达 0.85 V, 且在 140 h 以上的运行后仍保持 95.8% 的能量密度; 在碱性燃料电池中, 当 Pt 载量低至 0.017 mg cm −2 时, 其峰值功率密度达 508 mW cm −2 , 约为商用 5 wt% Pt/C (载量为 0.025 mg cm −2 ) 的两倍。本研究工作通过杂原子掺杂与合金化实现了电子结构与界面的协同调控, 为高性能氢/金属空气电池的开发提供了新思路。
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MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Dan Zhao,Jingjing Huang,Juan Li,Xiao Ma,Fang Wang,Honglei Zhang,Jiaze Xie,Jian Sun,Chuanxia Chen
Dual-readout protocols with two signal transduction channels can significantly improve the detection precision and diversity. In contrast to the previous tedious and time-consuming preparation of two response materials, a convenient and commercially available small molecule o -Phenylenediamine (OPD)-based dual-mode system was developed for the ultrasensitive detection of D-penicillamine (D-PA). Fortunately and interestingly, it is demonstrated that chloride ions (Cl − ) could largely enhance the oxidative ability of copper ions (Cu 2+ ) and improve the reaction rate and efficiency of the Cu 2+ -OPD system, leading to the boosted colorimetric and fluorescent signals and shorter reaction time. Meanwhile, D-PA could strongly bind with Cu 2+ to form a stable Cu 2+ -D-PA complex, thereby inhibiting the aforementioned oxidation process and exhibiting decrease of absorbance values and fluorescence intensity. Inspired by the excellent analytical performance, an “INH” molecular logic gate was devised by employing Cl − and D-PA as the inputs, and the dual-mode strategy has been successfully employed to probe D-PA in tablets and human serum samples. Such cost-effective yet efficacious analytical tool not only improve the sensing performance of the Cu 2+ -OPD reaction system, but also enhance the detection sensitivity for D-PA and promote the application and development of OPD-based reactions.
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ACS Omega
·
2023
·
IF 4.1
Yue Zhao,Chaoyuan Tang,Jingyi Huang,Hongyan Zhang,Jingbin Shi,Shujun Xu,Lisha Ma,Chun Peng,Qi Liu,Yang Xiong
In this study, we established a simple and rapid in vitro method for screening multidrug resistance (MDR) reversal agents in traditional Chinese medicines (TCMs), which could better correspond to the MDR reversing effect in vivo. Here, D-luciferin, a substrate for the enzyme firefly luciferase and also a substrate for ATP-binding cassette transporters (ABC transporters), was used as the probe to detect its efflux kinetics caused by ABC transporters. First, we established a stable doxorubicin (DOX)-resistant cell line (MCF-7/DOXFluc) that overexpressed luciferase. Then, some kinds of TCMs were chosen for the MDR reversal agents to measure its effect on inhibiting the D-luciferin outflow from MCF-7/DOXFluc, and the ideal reversal agent with the least D-luciferin efflux from MCF-7/DOXFluc was selected to further investigate its effect combined with DOX on MCF-7/DOXFluc tumor-bearing mice. The results indicated that quercetin (Qu) could remarkably increase the retention of D-luciferin in MCF-7/DOXFlucin vitro and in vivo. Also, the combination of Qu and DOX could exceedingly inhibit the tumor growth, which proved the feasibility of this in vitro screening method. The study proposed a feasible method for mass screening of MDR agents from TCMs in vitro.
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高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2022
·Vol. 29
·期 16
·e202203524
·
IF 5.02
Liang Yan,Yongqi Ke,Yu Wang,Jingkui Yang,Yujian He,Li Wu
Graphical A series of mini-PEGs modified D-amino acid-containing peptides were synthesized. It was determined that the polyethylene glycol (PEG) modification promoted the enzymatic cleavage of the D-amino acid-containing peptides under the action of serine-like proteases. It was previously reported that D-amino acid-containing peptides exhibited the ability to resist enzymatic hydrolysis. This study investigated the influence of mini-PEGs modification on enzymatic hydrolysis ability of D-amino acid-containing peptides. The results showed that PEGylation promoted enzymatic hydrolysis of the D-amino acid-containing peptide, especially, the cleavage rate of the D-amino acid-containing peptide 6-w with PEG 3 modification at the N-ends was up to 17 times higher in the presence of proteinase K (PROK) compared to those without PEG 3 modification. Moreover, analysis of the enzymatic cleavage sites demonstrated a similar cleavage pattern of the PEGylated D-amino acid-containing peptide to that of the unmodified peptide. The computational simulations further showed that the enhanced enzymatic hydrolysis ability can be attributed to the strong interaction between PROK and the peptide after PEG 3 modification and the resulting formation of a mature catalytic triad structure.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Wan Liu,Meng Cheng,Fangying Yuan,Jiaying He,Yulin Feng,Yi Jin,Jianfang Feng,Shilin Yang,Liangxing Tu
Nanocrystal technology is a common strategy to enhance the oral bioavailability of poorly-soluble drugs, and how to enlarge the enhancing ability on oral absorption via nanocrystals has become a pharmaceutical research field. In this study, we selected andrographolide (ADR), a typical poorly-soluble drug, as the model drug, and employed sodium dodecyl sulfate and D-ɑ-Tocopherol polyethylene glycol 1000 succinate copolymer (SDS-TPGS) to construct novel SDS-TPGS modified andrographolide nanocrystals (S-TANs). We found that the particle sizes of SDS/TPGS physical mixture modified nanocrystals (STANs) were about 553.4 ± 4.1 nm , while that of SDS-TPGS modified nanocrystals (S-TANs) were 604.6 ± 5.7 nm. During the production process of nanocrystals, the crystallinity of ADR was slightly decreased. Nanocrystals enhanced the saturation solubility of ADR, and improved oral absorption, C max and AUC 0-∞ of S-TANs was about 2.10 and 1.24 fold increased, STAN about 2.63, 2.97 fold. In a Real-time distribution study, we found the distribution time of andrographolide, SDS and TPGS in SDS/TPGS physical mixture modified nanocrystals system varies widely, while the distribution times of these materials were similar in SDS-TPGS copolymer modified nanocrystals system. These results indicated that modified with functional stabilizer was an efficient strategy to enlarge the enhancing ability on oral absorption of nanocrystals, and synthesized copolymer containing different functional stabilizers showed higher ability on enhancing oral bioavailability than physical mixtures.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 4
·1651-1662
·
IF 4.478
Zhang Wei,Zhang Yu,Liu Xin,Zhang Yue,Liu Yibing,Wang Wei,Su Rui,Sun Ying,Huang Yibing,Song Daqian,Wu Yanhua,Wang Xinghua
A sensing platform with both ratiometric fluorescence and colorimetric responses towards copper(II) ions (Cu 2+ ) and D-penicillamine (D-pen) was constructed based on carbon dots (CDs). o -Phenylenediamine (OPD) was employed as a chromogenic development reagent for reaction with Cu 2+ to generate the oxidation product 2,3-diaminophenazine (oxOPD), which not only emits green fluorescence at 555 nm, but also quenches the blue fluorescence of CDs at 443 nm via the inner filter effect (IFE) and Förster resonance energy transfer (FRET). Additionally, oxOPD exhibits obvious absorption at 420 nm. Since the intense chelation affinity of D-pen to Cu 2+ greatly inhibits the oxidation of OPD, the intensity ratio of fluorescence at 443 nm to that at 555 nm ( F 443 / F 555 ) and the absorbance at 420 nm ( A 420 ) were conveniently employed as spectral response signals to represent the amount of D-pen introduced into the testing system. This dual-signal sensing platform exhibits excellent selectivity and sensitivity towards both Cu 2+ and D-pen, with low detection limits of 0.019 μM and 0.092 μM, respectively. In addition, the low cytotoxicity of the testing reagents involved in the proposed sensing platform facilitates its application for live cell imaging. Graphical abstract
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危险品化学品经营许可证（带存储）